CN105724055A - Method for utilizing needle mushroom dregs to improve agaricus bisporus yield - Google Patents
Method for utilizing needle mushroom dregs to improve agaricus bisporus yield Download PDFInfo
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Abstract
The invention relates to a method for utilizing needle mushroom dregs to improve the agaricus bisporus yield. The method includes the following steps that 1, an agaricus bisporus earthing material is prepared, a pH is regulated, and the agaricus bisporus earthing material is adopted for earthing; 2, mushroom-dreg anaerobic fermentation extracting liquid is sprayed on the next day, the moisture content of the earthing material is regulated, and field management is performed; 3, an agaricus bisporus earthing material is supplemented, the anaerobic fermentation extracting liquid is sprayed, clear water is sprayed, and then fruiting management is performed; 4, when agaricus bisporus is damp, the mushroom dreg anaerobic fermentation extracting liquid is sprayed, and then follow-up management is performed by combining with clear water spraying. The method performs processing by combining with the characteristics of the needle mushroom dregs to improve the agaricus bisporus yield, effectively promotes re-utilization of the mushroom dregs and has an important significance on development of green agriculture and circular agriculture and protection the ecological environment.
Description
Technical field
The present invention relates to a kind of method utilizing needle mushroom dreg to improve Agaricus bisporus yield, belong to edible fungus technical field.
Background technology
Agaricus bisporus is the rotten type edible fungi of grass that artificial culture is the most extensively, total output is the highest, consumption figure is maximum in the world, and the mushrooms that Ye Shi China cultivated area is relatively big and foreign exchange earning is maximum is rich in several amino acids, vitamin etc., nutritious, delicious in taste.At present, the yield of China's factory culture Agaricus bisporus is 22～28kg/m2, when planting material consumption, cover soil material are constant, yield room for promotion is limited.Earthing is the essential condition of Agaricus bisporus fruiting, and the stimulation ability fruiting of Agaricus bisporus certain micro-organisms and chemokines in soil, therefore the quality of earthing directly determines yield, and this is the key breakthrough points making Agaricus bisporus increase production.
China is Edible Fungi state maximum in the world, and edible fungi annual production in 2013 reaches 31,700,000 tons, accounts for more than the 70% of Gross World Product, produces dry dreg about more than 2,000 ten thousand tons, enormous amount.The Edible Fungi that refers to edible fungi residue is remaining after terminating comprises mycelial compost, recycling problem at China's edible fungi residue is never well solved, dreg year, comprehensive utilization ratio was relatively low, major part dreg is arbitrarily stacked or is directly burned, not only cause the wasting of resources and environmental pollution, and it is easily caused mycete and pest infestation, bring potential safety hazard to Edible Fungi.
Edible fungus culturing raw material is by a series of biotransformations such as the biological nitrogen fixation of edible fungi thalline, zymolysis, and cellulose, hemicellulose, lignin etc. are degraded to some extent, makes in dreg containing abundant nutritional labeling and active substance.For batch production needle mushroom dreg, detect according to Ministry of Agriculture's food quality supervision verification test center (Jinan), containing 17 seed amino acids in batch production needle mushroom dreg, total amount reaches 8.49%, also containing 11.29% crude protein, 2.0% crude fat, 14.27% crude fibre, 1.77% calcium, 1.30% nitrogen, 0.72% phosphorus, 0.78% potassium, nutritious.
Therefore, edible fungi residue is nutritious, containing being eaten the nutrient substance that bacterium mycelia directly absorbs in a large number.Research shows, edible fungi residue particularly factory edible fungi dreg can again act as the raw material of edible fungus culturing, in the cultivating bisporous mushroom of dreg, it is entitled as the article (" edible and medical fungi " of " the cultivating bisporous mushroom test of batch production needle mushroom dreg tunnel-type fermenting ", 21st phase, 110th 111 page, 2013) disclose and utilize the method that batch production needle mushroom dreg ferment in second time material is the cultivating bisporous mushroom of major ingredient, yield can improve 12%.
The method of Chinese patent literature CN1039800611A (application number 201410247438.X) Shen Qing Publication a kind of Pleurotus eryngii cultivating bisporous mushroom of dreg, cultivation matrix adds the Pleurotus eryngii dreg of 50%～60%, compared with factory formula, cost of material can reduce by 50%～55%, and yield and exterior quality are superior to existing factory formula.
But dreg is directly used as planting material and there is also many problems: (1) generally selects factory edible fungi dreg, and dreg nutrition and physicochemical character after peasant household's culturing edible fungus are poor.(2) dreg kind is many, and quality is uneven, and nutrient content is not quite similar, and adding proportion is more difficult to be determined.(3) deal with improperly as culturing raw material and can cause that dreg is tacky, granule is in small, broken bits, poor air permeability, affect mycelial growth and fruiting.(4) cultivation later stage nutrient supply is not enough, causes that edible fungi yield and quality declines.(5) dreg easily grows miscellaneous bacteria and pathogen, causes the potential safety hazards such as bacterium bag pollution.(6) cost of transportation is high.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, it is provided that a kind of method utilizing needle mushroom dreg to improve Agaricus bisporus yield.The method utilizes needle mushroom dreg to prepare anaerobism lixiviating solution, leaching residual thing is added in earthing, and in the critical period of Agaricus bisporus growth, anaerobism lixiviating solution is sprayed on earthing, the purpose that Agaricus bisporus yield and quality improves can be realized, the fully utilized of dreg can be promoted again.
Technical scheme is as follows:
A kind of method improving Agaricus bisporus yield, comprises the steps:
(1) preparing agaricus bisporus soil covering material by the dry ratio of dreg anaerobic fermentation leaching residual thing 30%～50%, thin field soil 20%～40% and turfy soil 20%～40%, adding Calx adjustment pH is 7.2～7.5；After planting 15～20d, uses agaricus bisporus soil covering material to carry out earthing when mycelia material feeding 60%～70%, and thickness of earth covering is 2.5～3.5cm；
(2) earthing second day, according to 400～600mL/m2The amount of spraying spray dreg anaerobic fermentation lixiviating solution, regulate earthing water content, carry out field management；
(3) when 70%～90% bed surface mycelia grows to soil layer surface, 0.4～0.6cm thickness step (1) agaricus bisporus soil covering material prepared is mended；During water spray, first by 400～600mL/m2The amount of spraying spray anaerobism lixiviating solution, then spray clear water, comprehensive injection flow rate is about 2kg/m2, then carry out management of producing mushroom；
(4) when Agaricus bisporus change of tide, by 400～600mL/m2The amount of spraying spray dreg anaerobic fermentation lixiviating solution, in conjunction with spraying clear water, comprehensive injection flow rate is 1.5kg/m2, carry out follow-up management.
According to currently preferred, in described step (1), before preparation agaricus bisporus soil covering material, also include the sterilization of Agaricus bisporus mushroom house, strain making, culturing raw material are made, inoculate the step sending out bacterium.Agaricus bisporus mushroom house is sterilized, strain makes, culturing raw material makes, inoculation is sent out bacterium step and all can be adopted the ordinary skill in the art.
According to currently preferred, in described step (1), earthing water content is 18%～22% (mass percent).
The preparation process of above-mentioned dreg anaerobic fermentation lixiviating solution and dreg anaerobic fermentation leaching residual thing is as follows:
I needle mushroom dreg that is pollution-free and that go mouldy after the removal of impurity, pulverizing, is prepared pretreatment dreg by ()；
(ii) water content of the pretreatment dreg that regulating step (i) is prepared to 55%～65%, pH6.5～8.0, build heap ventilation of punching, ferment；When material temperature reaches more than 60 DEG C, carry out a turning, and add microbial bacterial agent by the mass ratio of 0.5～1.5 ‰, continue fermentation；When material temperature reaches more than 60 DEG C again, carrying out second time turning every other day, once every 48h turning, fermentation period is 18～22 days later, and fermentation ends adds quick lime and regulates pH7.5～8.0, prepares fermentation dreg；
(iii) by fermentation dreg prepared for step (ii) and water 1:(6～8 in mass ratio) ratio mix, anaerobic fermentation 45～50h, cross leaching filtrate, adjust pH value 7.5～8.0, preparing dreg anaerobic fermentation lixiviating solution, filtering residue is dreg anaerobic fermentation leaching residual thing.
According to currently preferred, in described step (i), the removal of impurity is the plastic bag in removal needle mushroom dreg, scleroma block, wooden stick, plastic foil, tie string.
According to currently preferred, in described step (ii), the heap building heap is high 0.8m～1.2m, bottom width 2.0m～2.5m, top width 1.0m～1.5m, the length trapezoidal bar buttress heap more than 3.0m, and heap body end face interval 50cm vertically uniformly makes passage on earth, and aperture is about 3cm.
In above-mentioned steps (ii), microbial bacterial agent can be commercially available stalk fermentation microbial bacterial agent product, the organic matter decomposing inoculant (2kg dress) that such as Shandong love Promised Land bio tech ltd produces, the stalk type organic matter decomposing inoculant (2kg dress) that Zhongnong Lvkang (Beijing) Biotechnology Co., Ltd. produces.
Beneficial effect
1, the present invention is with needle mushroom dreg for raw material, by aerobic solid-state fermentation and anaerobism liquid fermentation lixiviate, prepares dreg anaerobic fermentation lixiviating solution and leaching residual thing, containing substantial amounts of organic matter, various trace elements and humic acid material.Not containing only the nutrient that Agaricus bisporus growth is required in anaerobic fermentation lixiviating solution, also contain many beneficial microbes and microbial metabolic products, these metabolites are inhibited to some pathogen and miscellaneous bacteria；Leaching residual thing is used as cover soil material, can improve the physicochemical character of earthing, promote the formation of its crumb structure, and activating microorganisms is movable, is more conducive to fruiting, improves yield.
2, the present invention by adding leaching residual thing in earthing, and in three critical periods of Agaricus bisporus growth, anaerobism lixiviating solution is sprayed on earthing, Agaricus bisporus can be made to shift to an earlier date squaring period, improve the yield and quality, reduce the probability of bacteria infection and disease, reduce use and the recruitment cost of antibacterial.
3, the present invention in conjunction with the feature of needle mushroom dreg after treatment for improving Agaricus bisporus yield, be effectively promoted dreg again with, to development green agriculture and circular agriculture, preserve the ecological environment significant.
Detailed description of the invention
Below in conjunction with following example, technical scheme is described further, so that the purpose of the present invention, technical scheme and beneficial effect become apparent from.
The strain selected in embodiment is Agaricus bisporus 258, is purchased from Fu Bang Bacteria Co., Ltd. of Shen county of Shandong.
Planting type is factory culture, and culturing raw material adopts the ferment in second time material of conventional wheat straw and chicken manure, every square metre of materials 100kg.
Microbial bacterial agent is purchased from love Promised Land, Shandong bio tech ltd.
Embodiment 1
Dreg raw material is batch production needle mushroom dreg and Cotton Varieties by Small Farming Households needle mushroom dreg, mixes in quality 1:1 ratio.Batch production needle mushroom dreg is with corn cob and wood flour for Main Cultivation raw material, and Cotton Varieties by Small Farming Households needle mushroom dreg is with cotton seed hulls and wood flour for Main Cultivation raw material.
A kind of method improving Agaricus bisporus yield, step is as follows:
(1) Agaricus bisporus mushroom house sterilization, strain make, culturing raw material makes, inoculation is sent out bacterium and managed according to a conventional method；By the dry ratio preparation agaricus bisporus soil covering material of dreg anaerobic fermentation leaching residual thing 40%, thin field soil 30% and turfy soil 30%, add Calx and be adjusted to pH7.3；After planting 16d, uses agaricus bisporus soil covering material to carry out earthing when mycelia material feeding 60%～70%, and thickness of earth covering is 3.0cm；
(2) earthing second day, according to 500mL/m2The amount of spraying spray dreg anaerobism lixiviating solution, in conjunction with water spray and ventilate, regulate earthing water content be 20%, carry out field management according to a conventional method；
(3) when 70%～90% bed surface mycelia grows to soil layer surface, 0.5cm thickness step (1) agaricus bisporus soil covering material prepared is mended；During water spray, first press 500mL/m2The amount of spraying spray anaerobism lixiviating solution, then spray clear water, comprehensive injection flow rate is 2kg/m2, clear water sprays for three times in early, middle and late point, then carries out management of producing mushroom；
(4) gather altogether three tide mushrooms, when Agaricus bisporus change of tide, by 500mL/m2The amount of spraying spray dreg anaerobism lixiviating solution, in conjunction with spraying clear water, comprehensive injection flow rate is 1.5kg/m2, clear water sprays for three times in early, middle and late point, carries out follow-up Routine Management.
The preparation process of above-mentioned anaerobism lixiviating solution and leaching residual thing is as follows:
I needle mushroom dreg that is pollution-free and that go mouldy is pulverized and sieved by (), remove the plastic bag in dreg, scleroma block, wooden stick, plastic foil, tie string and other foreign material, prepare pretreatment dreg；
(ii) water content of the pretreatment dreg that regulating step (i) is prepared is to 60%, the artificial trapezoidal bar buttress heap that dreg is built up high 1.0m, bottom width 2.2m, top width 1.2m, heap body end face interval 50cm vertically uniformly makes passage on earth with thick waddy, and aperture is about 3cm, ferments；Build heap fermentation the 3rd day, material temperature reaches 60 DEG C, carries out a turning and adds microbial bacterial agent by the mass ratio of 1.0 ‰, continuing fermentation；Within 4th day, material temperature reaches more than 60 DEG C again, carries out second time turning every other day, then every 48h turning once, all needs suitable water transfer after each turning；Fermentation period is 20 days, and fermentation ends adds quick lime and regulates pH7.8, prepares fermentation dreg；
(iii) ratio of fermentation dreg prepared for part steps (ii) with water 1:7 in mass ratio is mixed, load in clean wide-mouth Plastic Drum, it is placed in shady place, closing anaerobic fermentation 48h, two-layer filtered through gauze, take filtrate, adjust pH value 7.8, preparing dreg anaerobic fermentation lixiviating solution, filtering residue is dreg anaerobic fermentation leaching residual thing, is placed at 4 DEG C standby.
Embodiment 2
Dreg raw material is Cotton Varieties by Small Farming Households needle mushroom dreg, and dreg is with cotton seed hulls and wood flour for Main Cultivation raw material.
A kind of method improving Agaricus bisporus yield, step is as follows:
(1) Agaricus bisporus mushroom house sterilization, strain make, culturing raw material makes, inoculation is sent out bacterium and managed according to a conventional method；By the dry ratio preparation agaricus bisporus soil covering material of dreg anaerobic fermentation leaching residual thing 30%, thin field soil 30% and turfy soil 40%, add Calx and be adjusted to pH7.5；After planting 18d, uses agaricus bisporus soil covering material to carry out earthing when mycelia material feeding 60%～70%, and thickness of earth covering is 3.0cm；
(2) earthing second day, according to 600mL/m2The amount of spraying spray dreg anaerobism lixiviating solution, in conjunction with water spray and ventilate, regulate earthing water content be 18%, carry out field management according to a conventional method；
(3) when 70%～90% bed surface mycelia grows to soil layer surface, mend 0.4cm thickness step (1) agaricus bisporus soil covering material prepared, during water spray, first press 600mL/m2The amount of spraying spray anaerobism lixiviating solution, then spray clear water, comprehensive injection flow rate is about 2kg/m2, clear water sprays for three times in early, middle and late point, then carries out management of producing mushroom；
(4) gather altogether three tide mushrooms, when Agaricus bisporus change of tide, by 600mL/m2The amount of spraying spray dreg anaerobism lixiviating solution, in conjunction with spraying clear water, comprehensive injection flow rate is 1.5kg/m2, clear water sprays for three times in early, middle and late point, carries out follow-up Routine Management.
The preparation process of above-mentioned anaerobism lixiviating solution and leaching residual thing is as follows:
I needle mushroom dreg that is pollution-free and that go mouldy is pulverized and sieved by (), remove the plastic bag in dreg, scleroma block, wooden stick, plastic foil, tie string and other foreign material, prepare pretreatment dreg；
(ii) water content of the pretreatment dreg that regulating step (i) is prepared is to 65%, the artificial trapezoidal bar buttress heap that dreg is built up high 0.8m, bottom width 2.0m, top width 1.0m, heap body end face interval 50cm vertically uniformly makes passage on earth with thick waddy, and aperture is about 3cm, ferments；Build heap fermentation the 3rd day, material temperature reaches 60 DEG C, carries out a turning and adds microbial bacterial agent by the mass ratio of 1.5 ‰, continuing fermentation；Within 4th day, material temperature reaches more than 60 DEG C again, carries out second time turning every other day, then every 48h turning once, all needs suitable water transfer after each turning；Fermentation period is 18 days, and fermentation ends adds quick lime and regulates pH8.0, prepares fermentation dreg；
(iii) ratio of fermentation dreg prepared for part steps (ii) with water 1:6 in mass ratio is mixed, load in clean wide-mouth Plastic Drum, it is placed in shady place, closing anaerobic fermentation 45h, two-layer filtered through gauze, take filtrate, adjust pH value 8.0, preparing dreg anaerobic fermentation lixiviating solution, filtering residue is dreg anaerobic fermentation leaching residual thing, is placed at 4 DEG C standby.
Embodiment 3
Dreg raw material is batch production needle mushroom dreg, and dreg is with corn cob and wood flour for Main Cultivation raw material.
A kind of method improving Agaricus bisporus yield, step is as follows:
(1) Agaricus bisporus mushroom house sterilization, strain make, culturing raw material makes, inoculation is sent out bacterium and managed according to a conventional method；By the dry ratio preparation agaricus bisporus soil covering material of dreg anaerobic fermentation leaching residual thing 50%, thin field soil 30% and turfy soil 20%, add Calx and be adjusted to pH7.2；After planting 15d, uses agaricus bisporus soil covering material to carry out when mycelia material feeding 60%～70%, and thickness of earth covering is 3.0cm；
(2) earthing second day, according to 400mL/m2The amount of spraying spray dreg anaerobism lixiviating solution, in conjunction with water spray and ventilate, regulate earthing water content be 22%, carry out field management according to a conventional method；
(3) when 70%～90% bed surface mycelia grows to soil layer surface, 0.6cm thickness step (1) agaricus bisporus soil covering material prepared is mended；400mL/m is first pressed during water spray2The amount of spraying spray anaerobism lixiviating solution, then spray clear water, comprehensive injection flow rate is about 2kg/m2, clear water sprays for three times in early, middle and late point, then carries out management of producing mushroom；
(4) gather altogether three tide mushrooms, when Agaricus bisporus change of tide, by 400mL/m2The amount of spraying spray dreg anaerobism lixiviating solution, in conjunction with spraying clear water, comprehensive injection flow rate is 1.5kg/m2, clear water sprays for three times in early, middle and late point, carries out follow-up Routine Management.
The preparation process of above-mentioned anaerobism lixiviating solution and leaching residual thing is as follows:
I needle mushroom dreg that is pollution-free and that go mouldy is pulverized and sieved by (), remove the plastic bag in dreg, scleroma block, wooden stick, plastic foil, tie string and other foreign material, prepare pretreatment dreg；
(ii) water content of the pretreatment dreg that regulating step (i) is prepared is to 55%, the artificial trapezoidal bar buttress heap that dreg is built up high 1.2m, bottom width 2.5m, top width 1.5m, heap body end face interval 50cm vertically uniformly makes passage on earth with thick waddy, and aperture is about 3cm, ferments；Build heap fermentation the 3rd day, material temperature reaches 60 DEG C, carries out a turning and adds microbial bacterial agent by the mass ratio of 0.5 ‰, continuing fermentation.Within 5th day, material temperature reaches more than 60 DEG C again, carries out second time turning every other day, then every 48h turning once, all needs suitable water transfer after each turning.Fermentation period is 22 days, and fermentation ends adds quick lime and regulates pH8.0, prepares fermentation dreg；
(iii) ratio of fermentation dreg prepared for part steps (ii) with water 1:8 in mass ratio is mixed, load in clean wide-mouth Plastic Drum, it is placed in shady place, closing anaerobic fermentation 50h, two-layer filtered through gauze, take filtrate, adjust pH value 7.5, preparing dreg anaerobic fermentation lixiviating solution, filtering residue is dreg anaerobic fermentation leaching residual thing, is placed at 4 DEG C standby.
Reference examples 1
Cultivation of agaricus bisporus method is with embodiment 1, the difference is that by 500mL/m2Amount sprays clear water and replaces spraying needle mushroom dreg anaerobic fermented liquid, and cover soil material is formulated by the dry ratio of thin field soil 30% and turfy soil 70%, and pH is 7.5.
Reference examples 2
Cultivation of agaricus bisporus method is with embodiment 1, the difference is that by 500mL/m2Amount sprays Pleurotus eryngii dreg extract and replaces spraying needle mushroom dreg anaerobic fermented liquid.
The preparation method of Pleurotus eryngii dreg extract is as follows:
(I) add the microbial bacterial agent of Pleurotus eryngii dreg quality 8%, regulate water content for pinch one with hands, have water to flow out between finger, but do not ooze and be as the criterion, room temperature sealing and fermenting 3 days, it is thus achieved that fermentation dreg.Fermentation microbial inoculum is the mass ratio 0.1:0.3:0.5 of cellulase, lignoenzyme and beer yeast.
(II) dreg after fermentation is mixed with pure water 1:10 in mass ratio, boil 35min, after filtration, obtain Pleurotus eryngii dreg extract.
Reference examples 3
Cultivation of agaricus bisporus method is with embodiment 1, the difference is that cover soil material by thin turfy soil 45%, needle mushroom dreg 45%, rice husk 5%, oil cake 3% and Calx 2% by weight formulated.The concrete preparation method of cover soil material is as follows:
(I) adjustment of feed moisture content: being humidified by turfy soil to water content is 30%, and dreg humidification is 45% to water content, and the water content of other raw material keeps naturalness；
(II) the pulverizing and sieving of raw material: by each raw material pulverizing of step (I) gained to below particle diameter 3cm.
(III) will through the turfy soil after step (II) pulverizes and sieves, dreg, rice husk and oil cake mix homogeneously by weight ratio.
(IV) adjustment of pH value: in the raw material through step (III) mix homogeneously, gradually adds Calx mix homogeneously by weight ratio on a small quantity, regulates pH value to 8.0.
Above example and reference examples, if three times are repeated, environment is consistent, results averaged.
Each reference examples agaricus bisporus soil covering all has a small amount of living contaminants, and each embodiment is all uninfected by miscellaneous bacteria, and budding Time transfer receiver 2～3 days morning, mushroom shape and reference examples indifference as a rule, Agaricus bisporus yield and sporophore protein content are significantly increased, and result is in Table 1.
Table 1 Agaricus bisporus yield and sporophore protein content
The above results shows, adds Flammulina velutiper (Fr.) Sing anaerobic fermentation leaching residual thing and sprays needle mushroom dreg on agaricus bisporus soil covering and detest anaerobic fermentation lixiviating solution and can improve Agaricus bisporus yield and quality, can be applicable on producing in earthing.
Claims (6)
1. the method improving Agaricus bisporus yield, comprises the steps:
(1) preparing agaricus bisporus soil covering material by the dry ratio of dreg anaerobic fermentation leaching residual thing 30%～50%, thin field soil 20%～40% and turfy soil 20%～40%, adding Calx adjustment pH is 7.2～7.5；After planting 15～20d, uses agaricus bisporus soil covering material to carry out earthing when mycelia material feeding 60%～70%, and thickness of earth covering is 2.5～3.5cm；
(2) earthing second day, according to 400～600mL/m2The amount of spraying spray dreg anaerobic fermentation lixiviating solution, regulate earthing water content, carry out field management；
(3) when 70%～90% bed surface mycelia grows to soil layer surface, 0.4～0.6cm thickness step (1) agaricus bisporus soil covering material prepared is mended；During water spray, first by 400～600mL/m2The amount of spraying spray anaerobism lixiviating solution, then spray clear water, comprehensive injection flow rate is about 2kg/m2, then carry out management of producing mushroom；
(4) when Agaricus bisporus change of tide, by 400～600mL/m2The amount of spraying spray dreg anaerobic fermentation lixiviating solution, in conjunction with spraying clear water, comprehensive injection flow rate is 1.5kg/m2, carry out follow-up management.
2. the method for claim 1, it is characterised in that in described step (1), before preparation agaricus bisporus soil covering material, also includes the sterilization of Agaricus bisporus mushroom house, strain making, culturing raw material are made, inoculate the step sending out bacterium.
3. the method for claim 1, it is characterised in that in described step (1), earthing water content is 18%～22% (mass percent).
4. the method for claim 1, it is characterised in that the preparation process of described dreg anaerobic fermentation lixiviating solution and dreg anaerobic fermentation leaching residual thing is as follows:
I needle mushroom dreg that is pollution-free and that go mouldy after the removal of impurity, pulverizing, is prepared pretreatment dreg by ()；
(ii) water content of the pretreatment dreg that regulating step (i) is prepared to 55%～65%, pH6.5～8.0, build heap ventilation of punching, ferment；When material temperature reaches more than 60 DEG C, carry out a turning, and add microbial bacterial agent by the mass ratio of 0.5～1.5 ‰, continue fermentation；When material temperature reaches more than 60 DEG C again, carrying out second time turning every other day, once every 48h turning, fermentation period is 18～22 days later, and fermentation ends adds quick lime and regulates pH7.5～8.0, prepares fermentation dreg；
(iii) by fermentation dreg prepared for step (ii) and water 1:(6～8 in mass ratio) ratio mix, anaerobic fermentation 45～50h, cross leaching filtrate, adjust pH value 7.5～8.0, preparing dreg anaerobic fermentation lixiviating solution, filtering residue is dreg anaerobic fermentation leaching residual thing.
5. method as claimed in claim 4, it is characterised in that in described step (i), the removal of impurity is the plastic bag in removal needle mushroom dreg, scleroma block, wooden stick, plastic foil, tie string.
6. method as claimed in claim 4, it is characterized in that, in described step (ii), the heap building heap is high 0.8m～1.2m, bottom width 2.0m～2.5m, top width 1.0m～1.5m, the length trapezoidal bar buttress heap more than 3.0m, heap body end face interval 50cm vertically uniformly makes passage on earth, and aperture is about 3cm.
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| CN106220298A (en) * | 2016-07-08 | 2016-12-14 | 山东省农业科学院农业资源与环境研究所 | A kind of improve mycelial growth rate and the agaricus bisporus soil covering of quality and preparation method |
| CN106278460A (en) * | 2016-08-09 | 2017-01-04 | 上海市农业科学院 | The method of needle mushroom dreg factory culture Agaricus bisporus and culture material formula thereof |
| CN108834746A (en) * | 2018-06-28 | 2018-11-20 | 河西学院 | A kind of edible mushroom cycle production process method |
| CN110122168A (en) * | 2019-05-28 | 2019-08-16 | 贵州省贵福菌业发展有限公司 | A kind of Pleurotus nebrodensis growth auxiliary agent and preparation method thereof |
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| CN106278460A (en) * | 2016-08-09 | 2017-01-04 | 上海市农业科学院 | The method of needle mushroom dreg factory culture Agaricus bisporus and culture material formula thereof |
| CN108834746A (en) * | 2018-06-28 | 2018-11-20 | 河西学院 | A kind of edible mushroom cycle production process method |
| CN110122168A (en) * | 2019-05-28 | 2019-08-16 | 贵州省贵福菌业发展有限公司 | A kind of Pleurotus nebrodensis growth auxiliary agent and preparation method thereof |
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| CN105724055B (en) | 2018-08-31 |
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Effective date of registration: 20190315 Address after: 272000 Houtun Village Residence, Xilipu Street Office, Rencheng District, Jining City, Shandong Province Patentee after: Jining Zhongzhong Agricultural Science and Technology Co., Ltd. Address before: 250100 No. 202 industrial North Road, Licheng District, Ji'nan, Shandong Patentee before: Shandong Academy of Agricultural Science, Institute of Agricultural Resources and Environment |
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Correction item: Patentee|Address Correct: Jining Zhongxin Agricultural Technology Co., Ltd|272000 Shandong city of Jining province Rencheng District twenty Shop Street office after the village resident False: Jining Zhongzhong Agricultural Science and Technology Co., Ltd.|272000 Shandong city of Jining province Rencheng District twenty Shop Street office after the village resident Number: 14-02 Volume: 35 |
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