CN110012779A - A method of culture medium of edible fungus is prepared using black soldier flies frass - Google Patents
A method of culture medium of edible fungus is prepared using black soldier flies frass Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention discloses a kind of methods for preparing culture medium of edible fungus using black soldier flies frass, belong to culture medium of edible fungus preparation technical field.For preparing in the prior art, culture medium of edible fungus cost is excessively high and the incomplete problem of nutriment, the present invention provides a kind of method for preparing culture medium of edible fungus using black soldier flies frass, it includes that undried black soldier flies frass is fermented, Bia4 bacterium solution is sprayed during fermentation, liquid portion at extruding is carried out after the completion of fermented, retain solid residue, solid residue is mixed evenly together with other raw materials, obtains culture medium of edible fungus after sterilized processing.The present invention is by being that raw material carries out preparing culture medium of edible fungus by black soldier flies frass, because nutriment is more in black soldier flies frass and cost is relatively low, and new approach is opened up for the application of black soldier flies, making its purposes be no longer limited to directly use is soil and fertilizer, black soldier flies frass economic benefit is obviously improved with using effect, whole preparation process is simple, easily operated.
Description
Technical field
The invention belongs to culture medium of edible fungus preparation technical fields, utilize black soldier flies frass more specifically to a kind of
The method for preparing culture medium of edible fungus.
Background technique
Edible mushroom refers to that fructification is very large, macro fungi that can be edible, is commonly referred to as mushroom.Edible mushroom known to China has
More than 350 kinds, wherein belong to Basidiomycotina more, common are: mushroom, mushroom, agaric, tremella, hedgehog hydnum, dictyophora phalloidea, is become less severe at straw mushroom
Mushroom, dried mushroom, russule, ganoderma lucidum, cordyceps sinensis, truffle, lark and bolete etc.；Minority belongs to Ascomycotina, wherein having: hickory chick,
Saddle fungus, ferfas etc..In these bacterium, there is log cultivation, it is the cultivation of excrement grass and substituting stuff cultivation, these planting types
Matrix edible mushroom produced is less to the effective component of body-care, also unsatisfactory to human body bring healthcare function.And
And since the most common compost of existing edible mushroom is cotton seed hulls, cotton seed hulls price rises steadily in recent years, makes edible bacterium
The increased costs of plant person, the plantation enthusiasm of serious contusion grower；Cheap substitute is found, just can guarantee planting edible mushroom
The sustainable development of industry.
It is also correspondingly improved regarding to the issue above, it is open such as Chinese Patent Application No. CN201710085005.2
Day is on October 23rd, 2018, and which disclose a kind of culture medium of edible fungus, the raw material of the culture medium is according to parts by weight
It calculates including component once: 20~40 parts of hardwood crumbs, 10~15 parts of bagasse, 5~15 parts of bean dregs, disodium hydrogen phosphate 10~30
Part, 5~15 parts of False flax grouts, 5~15 parts of kelp residue, 10~30 parts of cotton seed hulls, 5~10 parts of ionized calcium solvent, bamboo shoot shell 10~
30 parts, 5~10 parts of gibberellin, 1~5 part of vitamin, 2~8 parts of ammonium sulfate, 2~10 parts of yam flour, 2~10 parts of Prunella vulgaris, lemon
1~5 part of sour iron, 2~10 parts of dried silkworm chrysalis meal, 1~5 part of Radix Glycyrrhizae, 1~6 part of fermentation poultry dung.Using edible fungus culture medium of the present invention,
Cultivation period can be shortened, output increased enriches the nutrient raw material in base, ensure that the ample supply of raw material needed for strain, fits
For Pleurotus eryngii, needle mushroom, Pleurotus citrinopileatus, oyster mushroom, Pleurotus nebrodensis, mushroom, the edible mushroom culture of the multiple eatings bacterium such as agaric.The patent
Be disadvantageous in that: culture medium of edible fungus raw material is more, higher cost.
For another example Chinese Patent Application No. CN201010291827.4, publication date are on November 7th, 2012, which is related to one
Kind culture medium of edible fungus preparation method, it is to dry agricultural crop straw and crushed with pulverizer, the length of stalk after crushing
Degree is 1cm, be 80% by fresh potato slag water content with the agricultural crop straw that crushes is 3-5 according to dry weight meter quality proportioning:
7-5 adds 2% gypsum or calcium carbonate and the fungicide such as 0.1% carbendazim powder or 0.1% thiophanate methyl, in blender
In stir evenly, and be added limewash adjust mixing after material pH value be 6-8, by the mixture after above-mentioned two step process
Material, mechanically or manually packing mode and pouring into specification is in 17cm × 35cm × 0.045cm polypropylene plastics pocket, and bag height is about
17-23cm is sterilized 8-12 hours or high pressure sterilization 2-3 hours with normal temperature and pressure.The present invention is used for the cultivation and life of edible mushroom
It produces.Potato residues and agricultural crop straw are comprehensively utilized, not only solved the problems, such as the processing of potato residues but also provide production is high-quality
The culture medium of edible mushroom.The patent is disadvantageous in that: potato residues and stalk can have that stirring is insufficient, and make
Standby culture medium nutriment is not comprehensive.
Summary of the invention
1. to solve the problems, such as
For preparing in the prior art, culture medium of edible fungus cost is excessively high and the incomplete problem of nutriment, the present invention mention
For a kind of method for preparing culture medium of edible fungus using black soldier flies frass.The present invention is by being raw material system by black soldier flies frass
Standby culture medium of edible fungus opens up new way because nutriment is more in black soldier flies frass and cost is relatively low for the application of black soldier flies
Diameter is no longer limited to its purposes directly using being soil and fertilizer, and black soldier flies frass economic benefit and using effect have significantly
It is promoted, whole preparation process is simple, easily operated.
2. technical solution
To solve the above-mentioned problems, the technical solution adopted in the present invention is as follows:
A method of culture medium of edible fungus is prepared using black soldier flies frass, comprising the following steps:
(1) actinomyces straw medium is prepared, the streptomyces flocculus of inoculation self-sizing domestication after culture medium is sterilized
(Streptomyces flocculus) laboratory strains code name Bia4,37 DEG C obtain Bia4 bacterium in shaking table shake culture 20 hours
Liquid；
(2) undried black soldier flies frass is sieved, is placed it in large container, Bia4 bacterium is sprayed into large container
Liquid ferments 15 days under natural temperature；
(3) black soldier flies frass after fermentation, black soldier flies frass after fermentation is added to the water, temperature is higher than
60 DEG C are kept for 5 minutes or more, and discharging liquid by extruding body portion makes Liquid Fertilizer, retain remaining undissolved residue；
(4) by 2~2.5g of urea, 1~1.5g of ammonium hydrogen carbonate, 1~1.5g of magnesium sulfate, 6~8g of calcium sulfate, calcium superphosphate
Residue 1kg in 1.5~2g, 3~5g of molasses and above-mentioned steps (3) is mixed and is stirred, and is obtained after sterilized processing
Culture medium of edible fungus.
Further, actinomyces straw medium includes following component in the step (1): 60~100 mesh sieving straw
4~5g of stalk powder, 0.5~1g of magnesium sulfate, 0.05~0.1g of 1.5~2g of potassium dihydrogen phosphate, 1.5~2g of ammonium sulfate and ferrous sulfate,
Water 1kg.
Further, it is inoculated with after medium sterilization from the micro- yellowish-white streptomycete (Streptomyces of naturalized strain
Violascens) laboratory strains code name LPF or from the heat-resisting streptomycete (Streptomyces of naturalized strain
Thermotolerans) laboratory strains code name HFM, 37 DEG C shaking table shake culture 20 hours, obtain corresponding LPF bacterium solution or
HFM bacterium solution.
Further, in the step (2) water content of undried black soldier flies frass 35~40%.
Further, large container top is provided with ventilation opening, and large container outer wall is provided with insulating layer.
Further, into undried black soldier flies frass according to 1kg/m3Spray Bia4 bacterium solution.
Further, the quality of black soldier flies frass in the step (3) after fermentation and the mass ratio of water are
1:2。
Further, raw material obtains after pasteurization sterilizes after being mixed evenly in the step (4)
Culture medium of edible fungus.
Further, urea 2g, ammonium hydrogen carbonate 1g, magnesium sulfate 1.2g, calcium sulfate 8g, calcium superphosphate 2g in step (4),
Molasses 5g, residue 1kg.
3. beneficial effect
Compared with the prior art, the invention has the benefit that
(1) present invention is fermented using black soldier flies frass, squeezes reservation residue, finally carries out residue and other raw materials
Culture medium of edible fungus is obtained after sterilized processing is mixed evenly, manufacturing process is simple and convenient and preparation cost is lower；It is right
Black soldier flies frass carries out deep exploitation, new approach is opened up for the application of black soldier flies, so that no longer to the purposes of black soldier flies frass
It is confined to directly using being soil and fertilizer, so that black soldier flies frass economic benefit is obviously improved with using effect；
(2) undried black soldier flies frass is placed in large container by the present invention, and large container outer wall is provided with by protecting
Insulating layer made of adiabator provides the environment of an opposite heat preservation for the fermentation process of black soldier flies frass, accelerates Heisui River gadfly
The fermentation process of excrement；And large container top is provided with ventilation opening, guarantee the circulation of air, on the one hand avoids black soldier flies frass
Unpleasant decomposed odour is given out during the fermentation, on the other hand facilitates the need in the growth course of Bia4 bacterium solution to oxygen
It asks；Preferably, into undried black soldier flies frass according to 1kg/m3Fountain height spray Bia4 bacterium solution, fountain height cross conference wave
Take strain liquid, fountain height is too small, and object bacteria may not be able to will expect complete fermentation
(3) present invention is added by black soldier flies frass after fermentation with the quality of black soldier flies frass quality and water for 2:1
When entering into water, it not only can guarantee that subsequent extrusion process was gone on smoothly but also can water resource be made full use of, and do not caused to waste, more
Rationally；And the residue left and raw material be stirred uniformly after through pasteurization, pasteurization is a kind of to utilize lower temperature
Degree can not only kill germ but also be able to maintain the sterilization that nutriment flavor is constant in article, so that the edible mushroom culture being finally made
Without miscellaneous bacteria in base, nutriment is more comprehensive.
Detailed description of the invention
Fig. 1 is preparation process schematic diagram of the invention；
Specific embodiment
Black soldier flies (Hermetiaillucens.L) are a kind of septic insects, and larval feeding organic waste carries out ecology
Conversion, organic waste can be converted to insect protein and frass organic fertilizer, but all the time frass be all used directly for
Soil and fertilizer does not carry out going deep into constituent analysis and optimization operational version, and value of the product does not find full expression；Always with
Come, edible fungus culturing raw material is all based on sawdust, peanut shell, stalk and feces of livestock and poultry etc., lacks using black soldier flies frass as original
Material prepare the thinking of culture medium of edible fungus.Inventor is obtained in black soldier flies frass by being repeatedly repeatedly detected ingredient, detection
Solable matter accounts for 22.1%~24.2%, and after extracting solable matter, residual residue nutriment is abundant, can pass through
Allotment production, the high-quality culture medium raw material as soil type edible mushroom；Former black soldier flies frass by this method separation Liquid Fertilizer and
After preparing edible fungus culture medium, actual production value is more than doubled, and carries out deep exploitation to black soldier flies frass, is black
New approach is opened up in the application of stratiomyiid, so that being no longer limited to directly make using being soil and fertilizer to the purposes of black soldier flies frass
It obtains black soldier flies frass economic benefit to be obviously improved with using effect, be suitble to large-scale production and apply.
The present invention is further described below combined with specific embodiments below.
Embodiment 1
As shown in Figure 1, a kind of method for preparing culture medium of edible fungus using black soldier flies frass, comprising the following steps:
(1) actinomyces straw medium is prepared, the streptomyces flocculus of inoculation self-sizing domestication after culture medium is sterilized
(Streptomyces flocculus) laboratory strains code name Bia4,37 DEG C obtain Bia4 bacterium in shaking table shake culture 20 hours
Liquid；Preferably, which includes following component: 60~100 mesh sieving 4~5g of straw powder, and magnesium sulfate 0.5~
1g, 1.5~2g of potassium dihydrogen phosphate, 1.5~2g of ammonium sulfate, 0.05~0.1g of ferrous sulfate, water 1kg, so that the actinomyces stalk
Nutriment is more comprehensive in culture medium, so that Bia4 bacterium solution obtained is conducive to the fermentation of undried black soldier flies frass；And
For the culture medium through 121 DEG C, the high-temperature sterilization of 20min reduces the amount of miscellaneous bacteria, because miscellaneous bacteria can consume the nutriment in culture medium and
Some miscellaneous bacterias can inhibit the growth of object bacteria；Therefore the culture medium need to preferably be grown by high-temperature sterilization by target strain；Training
It can also be inoculated with after supporting base sterilizing from naturalized strain micro- yellowish-white streptomycete (Streptomyces violascens) laboratory strains generation
Number LPF is replaced from the heat-resisting streptomycete of naturalized strain (Streptomyces thermotolerans) laboratory strains code name HFM
Streptomyces flocculus (Streptomyces flocculus) the laboratory strains code name Bia4 of self-sizing domestication, 37 DEG C of shaking table concussions
Culture obtains LPF bacterium solution or HFM bacterium solution in 20 hours；LPF bacterium solution or HFM bacterium solution or Bia4 bacterium solution can sufficiently decompose black soldier flies
Fibre composition in frass is prepared to can be good in subsequent step releasing fulvic acid precursor；
(2) undried black soldier flies frass is sieved, the water content of undried black soldier flies frass is 35~40%, water content
All it is unfavorable for the fermentation of black soldier flies frass higher than 40% or lower than 35%；Undried black soldier flies frass is placed in large container
In；Preferably, large container top is provided with ventilation opening, guarantees the circulation of air, and black soldier flies frass is on the one hand avoided to ferment
Unpleasant decomposed odour is given out in the process, is on the other hand facilitated in the growth of Bia4 bacterium solution or LPF bacterium solution or HFM bacterium solution to oxygen
The demand of gas；Large container outer wall is provided with insulating layer, provides the ring of an opposite heat preservation for the fermentation process of black soldier flies frass
Border accelerates the fermentation process of black soldier flies frass；The insulating layer is the coating made of thermal insulation material；It is sprayed into large container
The bacterium solution as made from step 1 is spilt, is fermented 15 days under natural temperature；Preferably, bacterium solution fountain height made from the step 1 is
1kg/m3, fountain height cross conference waste strain liquid, fountain height is too small, and object bacteria may not be able to will expect complete fermentation；
(3) black soldier flies frass after fermentation, black soldier flies frass after fermentation is added to the water, to have fermented
The quality of black soldier flies frass after and the mass ratio of water are that black soldier flies frass after fermentation is added to the water by 1:2,
It can guarantee that subsequent extrusion process is gone on smoothly and can water resource be made full use of, do not cause to waste, it is relatively reasonable；Temperature is higher than
60 DEG C are kept for 5 minutes or more, and discharging liquid by extruding body portion makes Liquid Fertilizer, retain remaining undissolved residue；
(4) by 2~2.5g of urea, 1~1.5g of ammonium hydrogen carbonate, 1~1.5g of magnesium sulfate, 6~8g of calcium sulfate, calcium superphosphate
Residue 1kg in 1.5~2g, 3~5g of molasses and above-mentioned steps (3) is mixed and is stirred, and is obtained after sterilized processing
Culture medium of edible fungus；Preferably, sterilization treatment is carried out to raw material after mixing using pasteurization, pasteurization is
A kind of sterilization that can not only kill germ using lower temperature but also be able to maintain that nutriment flavor is constant in article, so that finally
Without miscellaneous bacteria in manufactured culture medium of edible fungus, nutriment is more comprehensive.
The present invention is fermented using black soldier flies frass, squeezes reservation residue, finally mixes residue and other raw materials
Conjunction has obtained culture medium of edible fungus after stirring evenly sterilized processing, and the culture medium of edible fungus manufacturing process is simple and convenient and prepares
Cost is relatively low；Culture medium of edible fungus obtained can be used for oyster mushroom, mushroom, needle mushroom, coprinus comatus, Hericium erinaceus, elegant precious mushroom, apricot Bao
The cultivation of the edible mushrooms such as mushroom, agrocybe, crab flavour mushroom, Pleurotus nebrodensis, black fungus, tremella；And the present invention carries out deeply black soldier flies frass
Degree utilizes, and opens up new approach for the application of black soldier flies, so that being no longer limited to direct use to the purposes of black soldier flies frass and being
Soil and fertilizer, so that black soldier flies frass economic benefit is obviously improved with using effect.
Embodiment 2
Substantially with embodiment 1, more specifically, the present embodiment prepares the method packet of culture medium of edible fungus using black soldier flies frass
Include following steps:
(1) actinomyces straw medium is prepared, which includes following component: 60 mesh sieving straw powder
4g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 1.5g, ammonium sulfate 1.5g, ferrous sulfate 0.05g, water 1kg, the actinomyces stalk culture
Base is through 121 DEG C, the streptomyces flocculus (Streptomyces flocculus) of inoculation self-sizing domestication after the high-temperature sterilization of 20min
Laboratory strains code name Bia4,37 DEG C obtain Bia4 bacterium solution in shaking table shake culture 20 hours；
(2) undried black soldier flies frass is sieved, the water content of undried black soldier flies frass is 35~40%, water content
All it is unfavorable for the fermentation of black soldier flies frass higher than 40% or lower than 35%；Undried black soldier flies frass is placed in large container
In；Preferably, large container top is provided with ventilation opening, guarantees the circulation of air, and black soldier flies frass is on the one hand avoided to ferment
Unpleasant decomposed odour is given out in the process, on the other hand facilitates the penetrating of Bia4 bacterium solution that it is made to be not easy to be sprayed onto external environment；Greatly
Type container outer wall is provided with insulating layer, provides the environment of an opposite heat preservation for the fermentation process of black soldier flies frass, accelerates Heisui River
The fermentation process of gadfly excrement；The insulating layer is the coating made of thermal insulation material；Into large container, sprinkling is by step 1
Bacterium solution obtained is fermented 15 days under natural temperature；Preferably, it is preferred that into undried black soldier flies frass according to 1kg/m3
Bia4 bacterium solution is sprayed, fountain height crosses conference waste strain liquid, and fountain height is too small, and object bacteria may not be able to will expect complete fermentation；
(3) black soldier flies frass after fermentation, black soldier flies frass after fermentation is added to the water, to have fermented
The quality of black soldier flies frass after and the mass ratio of water are that black soldier flies frass after fermentation is added to the water by 1:2,
Temperature is higher than 60 DEG C of holdings 5 minutes or more, and discharging liquid by extruding body portion makes Liquid Fertilizer, retains remaining undissolved residue；
(4) by urea 2g, ammonium hydrogen carbonate 1g, magnesium sulfate 1.2g, calcium sulfate 8g, calcium superphosphate 2g, molasses 5g, residue 1kg
It sterilizes to obtain culture medium of edible fungus through pasteurization after being mixed evenly.
Agaricus bisporus is inoculated on the culture medium of edible fungus, the humidity of control mycelium growth period compost is maintained at 60%
~65%, relative air humidity is controlled 80% or so, 22 DEG C~25 DEG C of growth temperature.Fruit body development stage compost temperature
60%~65%, earthing water content 20% or so, relative air humidity 85%~90%, fruit-body formation temperature 14 DEG C~16
℃；As control, another culture medium is prepared, which includes 1.2~1.5 parts of green grass clast, 1~1.5 part of cornstalk, chicken
0.5~0.5 part of excrement, 0.1~0.2 part of gypsum add water to be modulated into the culture medium of moisture content 60~65%, are inoculated with agaricus bisporus, other
Environment is identical as the culture medium of edible fungus using the preparation of black soldier flies frass；When the fruit body development of two groups of agaricus bisporus is to about 80%
When the stage, single body canopy size is counted, statistical result is as shown in table 1 below:
The fructification canopy size table of agaricus bisporus in 1 different culture medium of table
It can be seen that the culture medium of edible fungus that black soldier flies frass extracts production according to experiment statistics result, it is real in mature son
It is unobvious with control medium gap in body quantity, but to go out about 11% on the width of mature sporophore canopy greatly.Therefore, this hair
It is extracted in the bright slave black soldier flies frass and the culture medium of edible fungus of production has good cultivation effect, and preparation cost
It is low, it is suitble to large-scale promotion and application.
Example of the present invention is only that preferred embodiments of the present invention will be described, not to present inventive concept and
Range is defined, and under the premise of not departing from design philosophy of the present invention, this field engineers and technicians are to technology of the invention
The various changes and improvements that scheme is made should all fall into protection scope of the present invention.
Claims (9)
1. a kind of method for preparing culture medium of edible fungus using black soldier flies frass, it is characterised in that: the following steps are included:
(1) actinomyces straw medium is prepared, the streptomyces flocculus of inoculation self-sizing domestication, laboratory bacterium after culture medium is sterilized
Kind code name Bia4,37 DEG C obtain Bia4 bacterium solution in shaking table shake culture 20 hours；
(2) undried black soldier flies frass is sieved, is placed it in large container, Bia4 bacterium solution is sprayed into large container, from
It ferments 15 days at right temperature；
(3) black soldier flies frass after fermentation, black soldier flies frass after fermentation is added to the water, temperature be higher than 60 DEG C
Kept for 5 minutes or more, discharging liquid by extruding body portion makes Liquid Fertilizer, retains remaining undissolved residue；
(4) by 2~2.5g of urea, 1~1.5g of ammonium hydrogen carbonate, 1~1.5g of magnesium sulfate, 6~8g of calcium sulfate, calcium superphosphate 1.5~
Residue 1kg in 2g, 3~5g of molasses and above-mentioned steps (3) is mixed and is stirred, and obtains edible mushroom after sterilized processing
Culture medium.
2. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 1, it is characterised in that:
Actinomyces straw medium includes following component in the step (1): the sieving of 60~100 mesh 4~5g of straw powder, magnesium sulfate 0.5
~1g, 1.5~2g of potassium dihydrogen phosphate, 1.5~2g of ammonium sulfate and ferrous sulfate 0.05~0.1g, water 1kg.
3. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 2, it is characterised in that:
Inoculation is from the micro- yellowish-white streptomycete of naturalized strain, laboratory strains code name LPF or from the heat-resisting strepto- of naturalized strain after medium sterilization
Bacterium, laboratory strains code name HFM, 37 DEG C obtain corresponding LPF bacterium solution or HFM bacterium solution in shaking table shake culture 20 hours.
4. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 1, it is characterised in that:
The water content of undried black soldier flies frass is 35~40% in the step (2).
5. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 4, it is characterised in that:
Large container top is provided with ventilation opening, and large container outer wall is provided with insulating layer.
6. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 5, it is characterised in that:
Into undried black soldier flies frass according to 1kg/m3Spray Bia4 bacterium solution.
7. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 1, it is characterised in that:
The quality of black soldier flies frass in the step (3) after fermentation and the mass ratio of water are 1:2.
8. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 1, it is characterised in that:
Raw material obtains culture medium of edible fungus after pasteurization sterilizes after being mixed evenly in the step (4).
9. a kind of method for preparing culture medium of edible fungus using black soldier flies frass according to claim 8, it is characterised in that:
Urea 2g, ammonium hydrogen carbonate 1g, magnesium sulfate 1.2g, calcium sulfate 8g, calcium superphosphate 2g, molasses 5g, residue 1kg in step (4).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111280017A (en) * | 2020-04-10 | 2020-06-16 | 湖南省烟草公司长沙市公司宁乡市分公司 | Tobacco seedling cultivation nutrient soil, preparation method and application |
| CN116326421A (en) * | 2023-03-21 | 2023-06-27 | 北京市农林科学院 | Method for promoting yield increase of oyster mushrooms |
| WO2024102066A1 (en) * | 2022-11-07 | 2024-05-16 | Nutrition Technologies Research Pte. Ltd. | Biofertilizer compositions and methods for their use |
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