CN106399120B - A kind of ecological circulation cultural method of elegant precious mushroom - Google Patents

A kind of ecological circulation cultural method of elegant precious mushroom Download PDF

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Publication number
CN106399120B
CN106399120B CN201610441693.7A CN201610441693A CN106399120B CN 106399120 B CN106399120 B CN 106399120B CN 201610441693 A CN201610441693 A CN 201610441693A CN 106399120 B CN106399120 B CN 106399120B
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parts
bacterium bag
mushroom
fruiting
elegant precious
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CN106399120A (en
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王富根
陈再呜
潘飞云
余建妹
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HANGZHOU QIANDAOHU XINGBAO MUSHROOM INDUSTRY PROFESSIONAL COOPERATIVES
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HANGZHOU QIANDAOHU XINGBAO MUSHROOM INDUSTRY PROFESSIONAL COOPERATIVES
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

Abstract

The invention discloses inoculation, bacteria, long mushroom and waste gatherings on a kind of ecological circulation cultural method of elegant precious mushroom, including bacterium bag production, bacterium bag to recycle step.Culture raw material anxiety problem can be effectively relieved in the present invention, and culture cost of material is low, source is wide, full of nutrition, can mitigate environmental pollution, effectively prevent insect pest and significantly improve cultivating rate, and the cyclic utilization of waste, no waste generates, ecological, environmental protective.

Description

A kind of ecological circulation cultural method of elegant precious mushroom
Technical field
The present invention relates to elegant precious mushroom production technical field, in particular to the ecological circulation cultural method of a kind of elegant precious mushroom.
Background technique
Elegant precious mushroom, scientific name handle mushroom, belongs to one kind of oyster mushroom, and Eumycota, Basidiomycetes, agaric are belonged on taxology Mesh, Pleurotaceae, Pleurotus.This title of elegant precious mushroom derives from Taiwan, is a kind of denseer phoenix-tail mushroom of business taste.It is not It is same as common phoenix-tail mushroom, mushroom body is handsome small, and handle is 5-6 centimetres long, and lid diameter is less than 3 centimetres, so claiming elegant precious.
Elegant precious mushroom is a kind of natural edible bacterium full of nutrition, delicious flavour, and protein content is high, and quality is delicate, fiber Content is few;According to surveying and determination, protein 3.65~3.88%, crude fat 1.13~1.18%, reduced sugar 0.87 are contained in fresh mushroom ~1.80%, sugar 23.94~34.87%, lignin 2.64%, cellulose 12.85%, pectin 0.14%, also contain mine Substance element etc..Its protein content is 3~6 times higher than general vegetables close to meat.In addition, elegant precious mushroom also contains 17 In above amino acid, more valuable, it, which contains human body itself, to manufacture, and usually Soviet Union's ammonia of shortage in food Acid, lysine, leucine etc..As it can be seen that elegant precious mushroom is the nutraceutical of a kind of high protein, low fat, it is tasty, has Unique flavor calls it by the fine-sounding name of " monosodium glutamate mushroom ".
With the fast development of Edible Fungi, culturing raw material supply is becoming tight day, and only cotton seed hulls, straw etc. have been difficult to full Sufficient production requirement is sought to replace raw material to be the task of top priority, and ramulus mori is the byproduct of sericulture industry, and abundance is easy to collect, cost Cheap, a large amount of ramulus mori is not utilized effectively at present, is often to burn as firewood, environmental pollution is serious.
Hickory Juglandaceae hickory plant.Its fruit has high nutritive value and unique taste flavor. After hickory nut is mature, it is coated with two layers of shell of hickory nut shell and pecan shell.Pecan shell is by containing chloroplaset and arrangement Close parenchymal tissue layer and complicated vascular bundle network structure composition.Hickory nut shell is formed by lignifying lithocyte layer, is taken off In faint yellow when Pu shell, hickory nut is in brownish black after the processing technologys such as boiling frying.It is total that pecan shell accounts for hickory nut fruit 80% or so of weight is abandoned by accumulation everywhere in some hickory nut producing region Pu shells, is produced to traffic in local look of a village and village Very big influence.The black juice leaked out after the accumulation of pecan shell has very strong alkalinity and corrosivity, to water source and soil Produce serious pollution.
It is directly to spray water to carry out moisturizing processing to bacterium bag after fruiting when existing elegant precious mushroom culture, such short time humidification effect Fruit is good, and there are the long periods between fruiting twice, and as time goes by, moisture is easy to scatter and disappear, and has led to fruiting again When water deficient, moistening effect is poor, and cultivating rate is low, meanwhile, such processing fruiting end be still it is exposed, pest be easy into Enter, and eat up mycelium, leverages cultivating rate in this way.
Summary of the invention
The purpose of the present invention is to provide a kind of ecological circulation cultural methods of elegant precious mushroom, and it is tight that culture raw material can be effectively relieved Zhang Wenti, culture cost of material is low, source is wide, full of nutrition, can mitigate environmental pollution, effectively prevent insect pest and significantly improves out Mushroom rate, the cyclic utilization of waste, no waste generate, ecological, environmental protective.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of ecological circulation cultural method of elegant precious mushroom, including bacterium bag production, inoculation, bacteria, long mushroom and discard in bacterium bag Object collection recycles step, and the bacterium bag is made as adding water to be adjusted to water content 60-65% compost, is then charged into poly- third In alkene single port polybag, sterilize, it is cooling;The compost is mixed by following raw material by weight: 100 parts of ramulus mori powder, 15-20 parts of pecan shell crushed material, 10-15 parts of Chinese chestnut bud husk crushed material, 5-10 parts of corn flour, organic-inorganic nutrition complex 20-30 parts, 1-2 parts of lime.
Compost of the present invention is aided with pecan shell crushed material, Chinese chestnut bud husk crushed material, raw material using ramulus mori powder as main material It is at low cost, source is wide, full of nutrition, in this way cooperate be suitble to elegant precious mushroom growth, especially the present invention be added in compost Machine inorganic nutrients complex, organic-inorganic nutrition complex nutritional ingredient is reasonable, abundant, is suitble to the growth of elegant precious mushroom, has simultaneously Machine inorganic nutrients complex has porosity abundant, and moisturizing and the effect for keeping nutrient not to be lost are good.
Preferably, the organic-inorganic nutrition complex is mixed by following raw material components by weight: earthworm 100 parts of excrement, 30-50 parts of bulk kelp crushed material, 30-50 parts of fly maggot excrement, 20-30 parts of straw powder, 10-20 parts of bamboo vinegar, pine needle meal 10- 20 parts, 10-20 parts of molasses.
Organic-inorganic nutrition complex wants raw material based on wormcast, bulk kelp crushed material, and wormcast particle has very big Surface area, pore structure is good, moisturizing and keep nutrient effect it is good, meanwhile, wormcast rich in nutrition content itself, contained by battalion It forms point and microelement etc. is very suitable to the production of elegant precious mushroom.The small graininess of wormcast can also help to promote most with air It possibly contacts, they keep culture medium no longer hardened and hard after mixing with culture medium.Fly maggot excrement, bamboo vinegar, molasses nutrition are rich Richness, rich in substances such as organic matter and protein, straw powder, pine needle meal lignocellulosic are abundant, the compound body acupuncture of organic-inorganic nutrition Elegant precious mushroom nutritional need is designed, each component cooperates, and reasonable nutritional arrangment, collaboration plays a role.
Preferably, the organic-inorganic nutrition complex the preparation method is as follows:
(1) wormcast, bulk kelp crushed material, fly maggot excrement, straw powder, bamboo vinegar, pine needle meal and molasses are uniformly mixed Mixture is obtained, into mixture plus water is adjusted to water content and obtains fermentation substrate for 60-65%;
(2) composite bacteria liquid is added into fermentation substrate to be uniformly mixed, composite bacteria liquid dosage is mixture in step (1) The 5-10% of weight builds after heap fermentation to obtain fermentation material, builds the environment temperature control of heap fermentation at 20-30 DEG C, fermentation time 7-14 It;
(3) particle will be made after fermentation material drying, then particle will be heat-treated, it is compound to obtain organic-inorganic nutrition after cooling Body, the heat treatment keep 50-60min to be warming up to 180-200 DEG C with the heating rate of 2-5 DEG C/min.
The present invention first uses composite bacteria liquid to carry out biofermentation processing, can make fly maggot excrement, bamboo vinegar, straw powder, pine needle meal It is further glued with wormcast and bulk kelp crushed material, promote the nutriment of fly maggot excrement, bamboo vinegar, straw powder, pine needle meal to decompose At being easier to plant absorption ingredient, meanwhile, biofermentation processing can also kill harmful microorganism.Finally to obtained after fermentation Grain is heat-treated, so that it is compound and come to the surface to can promote organic and inorganic ingredient further combined with complex is formed for each ingredient Reaction and ion exchange are learned, is converted to fly maggot excrement similar to soil humic metallic substance, straw powder, pine needle meal are further converted into Ingredient is easily absorbed, relatively stable organic mineral complex is ultimately formed, plays efficient moisturizing, fertilizer conservation effect.
Preferably, the straw powder by corn stover, wheat stalk, soybean stalk, rape stalk, rice straw according to It is crushed after the mixing of 1:1:1:1:1 weight ratio.
Preferably, composite bacteria liquid mixed with Bacillus subtillis liquid according to the volume ratio of 1:0.5-0.6 by EM bacterium solution and At, viable count >=100,000,000/milliliter of EM bacterium solution, viable count >=100,000,000/milliliter of Bacillus subtillis liquid.
Preferably, during long mushroom, insect prevention activation processing is carried out to bacterium bag before fruiting again after fruiting of bacterium bag, Insect prevention is activated are as follows: and the fruiting end at the top of bacterium bag is submerged into vertically downward into insect prevention activating solution and is impregnated 10-12 hours, bacterium Submergence height of the fruiting end at bag top in insect prevention activating solution is in 2-3cm;Bacterium bag fruiting 7-8 times in total.
Fruiting refers to that mycelium grows up to mature elegant precious mushroom thallus and gathers in completion.At insect prevention activation of the invention Reason is that this period before fruiting carries out again after the completion of a fruiting, this section of period mycelium is easy by insect pest invasion. In the submergence height in insect prevention activating solution, the 2-3cm at the top of 2-3cm, that is, bacterium bag is immersed in insect prevention activation at fruiting end at the top of bacterium bag In liquid.
The present invention by being submerged at the fruiting end at the top of bacterium bag into insect prevention activating solution vertically downward (i.e. bacterium bag inversion) It impregnates 10-12 hours, it is open environment that bacterium bag, which only has fruiting end, now closes in insect prevention activating solution fruiting end seal, forms liquid The pest in seal ring border, outside cannot be introduced into, and the moisture of culture medium, which also locks, to be scattered and disappeared, and prolonged processing forms anoxic ring Border can effectively kill the pest in bacterium bag in culture medium.Meanwhile fruiting end is submerged into insect prevention activating solution in insect prevention, desinsection While effect, can effectively draws water, nutritional ingredient, moistening effect is good, substantially increases cultivating rate.
Preferably, the proportion of the insect prevention activating solution each component by weight is as follows: 1000 parts of water, potassium sulfate 2-4 Part, 3-6 parts of ammonium phosphate, 10-20 parts of bamboo vinegar, 20-30 parts of humic acid, 1-3 parts of inducer, 1-3 parts of promotor, vitamin C 3- 5 parts, 15-25 parts of Sargassum horneri extract.
Potassium sulfate, ammonium phosphate provide inorganic potash fertilizer, nitrogenous fertilizer and phosphate fertilizer;Bamboo vinegar, humic acid, which provide, full of nutrition to be had Machine is fertile, organo-mineral complexing in insect prevention activating solution, full of nutrition, can overcome the disadvantages that the loss for the nutrition that fruiting generates, and improves fruiting section Fertility, to effectively improve cultivating rate.
The present invention adds promotor in insect prevention activating solution, can improve cultivating rate, under the action of promotor, can promote show Precious mushroom utilizes the decomposition of nutritional ingredient lignocellulosic in bacterium bag, to improve yield and cultivating rate.The present invention is living in insect prevention Change in liquid and add inducer, act synergistically with promotor, improves cultivating rate.
The present invention adds bamboo vinegar in insect prevention activating solution, has permeability well, other components can be promoted to infiltrate through It plays a role in the culture medium at fruiting end, meanwhile, bamboo vinegar has good deodorizing effect, can eliminate in incubation and cultivate Stink, the peculiar smell of base generation.Meanwhile bamboo vinegar can also play the role of plant hormone, can improve cultivating rate, improve elegant precious mushroom Mouthfeel.
Sargassum horneri extract is added in insect prevention activating solution of the present invention, ingredient is mainly brown algae polyphenols, is had very strong anti- Oxidative function, energy sterilizing, while Sargassum horneri extract has extraordinary moisture-keeping efficacy, enables to fruiting after Immersion treatment The moistening effect at end maintains extraordinary state to promote the production of elegant precious mushroom to improve cultivating rate.Vitamin C has very strong Anti-oxidation function, moreover it is possible to nutrition is provided.The present invention is used cooperatively using the Sargassum horneri extract of high concentration with vitamin C, total Anti-oxidant aspect has good synergistic effect, and disinfection effect is good, and moistening effect is good, can effectively improve cultivating rate.
Preferably, the Sargassum horneri extract the preparation method is as follows: by Sargassum horneri crushed after being dried, by crushed material and 85% Ethyl alcohol is stirred 3-5 hours according to the solid-liquid ratio of 1g:15-20mL at 40-45 DEG C, and supernatant, vacuum decompression are collected in centrifugation The 10-15% for being concentrated into original volume obtains concentrate, and concentrate ethyl acetate extracts 3-5 times, and last evaporated under reduced pressure removes ethyl acetate Obtain Sargassum horneri extract.
Preferably, the inducer is diazosulfide, the promotor is oleic acid.
Preferably, waste gathering is recycling as collecting discarded bacterium bag and the discarded mushroom handle mushroom base of a fruit enters methane-generating pit life Produce biogas, the fuel of the biogas of production as the required heat source of bacterium bag sterilizing, the biogas slurry and biogas residue generated in methane-generating pit is as organic Fertilizer is planted for mulberry tree.
The beneficial effects of the present invention are: culture raw material anxiety problem can be effectively relieved, culture cost of material is low, source is wide, It is full of nutrition, environmental pollution can be mitigated, insect pest is effectively prevent and significantly improve cultivating rate, the cyclic utilization of waste, without discarded produce It is raw, ecological, environmental protective.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art. Method in following embodiments is unless otherwise instructed the conventional method of this field.
Embodiment 1:
Inoculation, bacteria (are supported at 23-25 DEG C on a kind of ecological circulation cultural method of elegant precious mushroom, including bacterium bag production, bacterium bag Bacterium, so that mycelia covers with bacterium bag), long mushroom (in greenhouse at 25-28 DEG C, being cultivated under humidity 85%-90%) and waste gathering circulation Using step, waste gathering is recycling as collecting discarded bacterium bag and the discarded mushroom handle mushroom base of a fruit enters methane-generating pit production biogas, The fuel of heat source needed for the biogas of production sterilizes as bacterium bag, the biogas slurry and biogas residue generated in methane-generating pit are used for mulberry as organic fertilizer Tree planting.The bacterium bag is made as adding water to be adjusted to water content 60-65% compost, is then charged into polypropylene single port polybag In, it sterilizes, it is cooling;The compost is mixed by following raw material by weight: 100 parts of ramulus mori powder, pecan shell powder It minces 15 parts, 15 parts of Chinese chestnut bud husk crushed material, 5 parts of corn flour, 30 parts of organic-inorganic nutrition complex, 2 parts of lime.
The organic-inorganic nutrition complex is mixed by following raw material components by weight: 100 parts of wormcast, huge 30 parts of algae crushed material, 50 parts of fly maggot excrement, 20 parts of straw powder, 20 parts of bamboo vinegar, 10 parts of pine needle meal, 20 parts of molasses.
The organic-inorganic nutrition complex the preparation method is as follows:
(1) wormcast, bulk kelp crushed material, fly maggot excrement, straw powder, bamboo vinegar, pine needle meal and molasses are uniformly mixed Mixture is obtained, into mixture plus water is adjusted to water content and obtains fermentation substrate for 60-65%;The straw powder is by corn stover, small Wheat Straw, soybean stalk, rape stalk, rice straw crush after mixing according to 1:1:1:1:1 weight ratio.
(2) composite bacteria liquid is added into fermentation substrate to be uniformly mixed, composite bacteria liquid dosage is mixture in step (1) The 5% of weight obtains fermentation material after building heap fermentation, and the environment temperature for building heap fermentation is controlled at 20 DEG C, fermentation time 14 days;Compound bacteria Liquid is mixed with Bacillus subtillis (commercially available) liquid according to the volume ratio of 1:0.5 by EM bacterium solution (commercially available), the viable bacteria of EM bacterium solution >=1 hundred million/milliliter of number, viable count >=100,000,000/milliliter of Bacillus subtillis liquid.
(3) particle will be made after fermentation material drying, then particle will be heat-treated, it is compound to obtain organic-inorganic nutrition after cooling Body, the heat treatment keep 60min to be warming up to 180 DEG C with the heating rate of 2 DEG C/min.
During long mushroom: carrying out insect prevention activation processing, insect prevention activation to bacterium bag before fruiting again after fruiting of bacterium bag Processing are as follows: the fruiting end at the top of bacterium bag is submerged into vertically downward into insect prevention activating solution and is impregnated 10 hours, going out at the top of bacterium bag Submergence height of the mushroom end in insect prevention activating solution is in 3cm;Fruiting-insect prevention activation processing-fruiting recycles progress in this way, and bacterium bag is in total Fruiting 7 times, insect prevention is activated 6 times totally.
The proportion of the insect prevention activating solution each component by weight is as follows: 1000 parts of water, 2 parts of potassium sulfate, and ammonium phosphate 3 Part, 20 parts of bamboo vinegar, 20 parts of humic acid, 1 part of diazosulfide, 3 parts of oleic acid, 5 parts of vitamin C, 25 parts of Sargassum horneri extract.Institute State Sargassum horneri extract the preparation method is as follows: by Sargassum horneri crushed after being dried, by crushed material and 85% ethyl alcohol according to the material of 1g:15mL Liquor ratio is stirred 5 hours at 40 DEG C, centrifugation, collects supernatant, the 10% of vacuum-concentrcted to original volume obtains concentrate, dense Contracting liquid ethyl acetate extracts 3 times, and last evaporated under reduced pressure removes ethyl acetate and obtains Sargassum horneri extract.
Embodiment 2:
Inoculation, bacteria (are supported at 23-25 DEG C on a kind of ecological circulation cultural method of elegant precious mushroom, including bacterium bag production, bacterium bag Bacterium, so that mycelia covers with bacterium bag), long mushroom (in greenhouse at 25-28 DEG C, being cultivated under humidity 85%-90%) and waste gathering circulation Using step, waste gathering is recycling as collecting discarded bacterium bag and the discarded mushroom handle mushroom base of a fruit enters methane-generating pit production biogas, The fuel of heat source needed for the biogas of production sterilizes as bacterium bag, the biogas slurry and biogas residue generated in methane-generating pit are used for mulberry as organic fertilizer Tree planting.The bacterium bag is made as adding water to be adjusted to water content 60-65% compost, is then charged into polypropylene single port polybag In, it sterilizes, it is cooling;The compost is mixed by following raw material by weight: 100 parts of ramulus mori powder, pecan shell powder It minces 20 parts, 10 parts of Chinese chestnut bud husk crushed material, 10 parts of corn flour, 20 parts of organic-inorganic nutrition complex, 1 part of lime.
The organic-inorganic nutrition complex is mixed by following raw material components by weight: 100 parts of wormcast, huge 50 parts of algae crushed material, 30 parts of fly maggot excrement, 30 parts of straw powder, 10 parts of bamboo vinegar, 20 parts of pine needle meal, 10 parts of molasses.
The organic-inorganic nutrition complex the preparation method is as follows:
(1) wormcast, bulk kelp crushed material, fly maggot excrement, straw powder, bamboo vinegar, pine needle meal and molasses are uniformly mixed Mixture is obtained, into mixture plus water is adjusted to water content and obtains fermentation substrate for 60-65%;The straw powder is by corn stover, small Wheat Straw, soybean stalk, rape stalk, rice straw crush after mixing according to 1:1:1:1:1 weight ratio.
(2) composite bacteria liquid is added into fermentation substrate to be uniformly mixed, composite bacteria liquid dosage is mixture in step (1) The 10% of weight obtains fermentation material after building heap fermentation, and the environment temperature for building heap fermentation is controlled at 30 DEG C, fermentation time 7 days;Compound bacteria Liquid is mixed with Bacillus subtillis (commercially available) liquid according to the volume ratio of 1:0.6 by EM bacterium solution (commercially available), the viable bacteria of EM bacterium solution >=1 hundred million/milliliter of number, viable count >=100,000,000/milliliter of Bacillus subtillis liquid.
(3) particle will be made after fermentation material drying, then particle will be heat-treated, it is compound to obtain organic-inorganic nutrition after cooling Body, the heat treatment keep 50min to be warming up to 200 DEG C with the heating rate of 5 DEG C/min.
During long mushroom: carrying out insect prevention activation processing, insect prevention activation to bacterium bag before fruiting again after fruiting of bacterium bag Processing are as follows: the fruiting end at the top of bacterium bag is submerged into vertically downward into insect prevention activating solution and is impregnated 12 hours, going out at the top of bacterium bag Submergence height of the mushroom end in insect prevention activating solution is in 2cm;Bacterium bag fruiting 8 times in total, insect prevention are activated 7 times totally.
The proportion of the insect prevention activating solution each component by weight is as follows: 1000 parts of water, 4 parts of potassium sulfate, and ammonium phosphate 6 Part, 10 parts of bamboo vinegar, 30 parts of humic acid, 3 parts of diazosulfide, 1 part of oleic acid, 3 parts of vitamin C, 15 parts of Sargassum horneri extract.Institute State Sargassum horneri extract the preparation method is as follows: by Sargassum horneri crushed after being dried, by crushed material and 85% ethyl alcohol according to the material of 1g:20mL Liquor ratio is stirred 3 hours at 45 DEG C, centrifugation, collects supernatant, the 15% of vacuum-concentrcted to original volume obtains concentrate, dense Contracting liquid ethyl acetate extracts 5 times, and last evaporated under reduced pressure removes ethyl acetate and obtains Sargassum horneri extract.
Embodiment 3:
Inoculation, bacteria (are supported at 23-25 DEG C on a kind of ecological circulation cultural method of elegant precious mushroom, including bacterium bag production, bacterium bag Bacterium, so that mycelia covers with bacterium bag), long mushroom (in greenhouse at 25-28 DEG C, being cultivated under humidity 85%-90%) and waste gathering circulation Using step, waste gathering is recycling as collecting discarded bacterium bag and the discarded mushroom handle mushroom base of a fruit enters methane-generating pit production biogas, The fuel of heat source needed for the biogas of production sterilizes as bacterium bag, the biogas slurry and biogas residue generated in methane-generating pit are used for mulberry as organic fertilizer Tree planting.The bacterium bag is made as adding water to be adjusted to water content 60-65% compost, is then charged into polypropylene single port polybag In, it sterilizes, it is cooling;The compost is mixed by following raw material by weight: 100 parts of ramulus mori powder, pecan shell powder It minces 18 parts, 12 parts of Chinese chestnut bud husk crushed material, 8 parts of corn flour, 25 parts of organic-inorganic nutrition complex, 1.5 parts of lime.
The organic-inorganic nutrition complex is mixed by following raw material components by weight: 100 parts of wormcast, huge 40 parts of algae crushed material, 40 parts of fly maggot excrement, 25 parts of straw powder, 15 parts of bamboo vinegar, 15 parts of pine needle meal, 15 parts of molasses.
The organic-inorganic nutrition complex the preparation method is as follows:
(1) wormcast, bulk kelp crushed material, fly maggot excrement, straw powder, bamboo vinegar, pine needle meal and molasses are uniformly mixed Mixture is obtained, into mixture plus water is adjusted to water content and obtains fermentation substrate for 60-65%;The straw powder is by corn stover, small Wheat Straw, soybean stalk, rape stalk, rice straw crush after mixing according to 1:1:1:1:1 weight ratio.
(2) composite bacteria liquid is added into fermentation substrate to be uniformly mixed, composite bacteria liquid dosage is mixture in step (1) The 8% of weight obtains fermentation material after building heap fermentation, and the environment temperature for building heap fermentation is controlled at 25 DEG C, fermentation time 10 days;Compound bacteria Liquid is mixed with Bacillus subtillis (commercially available) liquid according to the volume ratio of 1:0.5 by EM bacterium solution (commercially available), the viable bacteria of EM bacterium solution >=1 hundred million/milliliter of number, viable count >=100,000,000/milliliter of Bacillus subtillis liquid.
(3) particle will be made after fermentation material drying, then particle will be heat-treated, it is compound to obtain organic-inorganic nutrition after cooling Body, the heat treatment keep 55min to be warming up to 200 DEG C with the heating rate of 4 DEG C/min.
During long mushroom: carrying out insect prevention activation processing, insect prevention activation to bacterium bag before fruiting again after fruiting of bacterium bag Processing are as follows: the fruiting end at the top of bacterium bag is submerged into vertically downward into insect prevention activating solution and is impregnated 11 hours, going out at the top of bacterium bag Submergence height of the mushroom end in insect prevention activating solution is in 2.5cm;Bacterium bag fruiting 7 times in total, insect prevention are activated 6 times totally.
The proportion of the insect prevention activating solution each component by weight is as follows: 1000 parts of water, 3 parts of potassium sulfate, and ammonium phosphate 4 Part, 15 parts of bamboo vinegar, 25 parts of humic acid, 2 parts of diazosulfide, 2 parts of oleic acid, 4 parts of vitamin C, 20 parts of Sargassum horneri extract.Institute State Sargassum horneri extract the preparation method is as follows: by Sargassum horneri crushed after being dried, by crushed material and 85% ethyl alcohol according to the material of 1g:15mL Liquor ratio is stirred 4 hours at 42 DEG C, centrifugation, collects supernatant, the 15% of vacuum-concentrcted to original volume obtains concentrate, dense Contracting liquid ethyl acetate extracts 4 times, and last evaporated under reduced pressure removes ethyl acetate and obtains Sargassum horneri extract.
Culture raw material anxiety problem can be effectively relieved in the present invention, and culture cost of material is low, source is wide, full of nutrition, can subtract Light environmental pollution effectively prevent insect pest and significantly improves cultivating rate, of the invention compared with conventional shower water moisturizing processing Cultivating rate can be improved 20-30% by method, elegant precious mushroom output increased at least 15% or more, the cyclic utilization of waste, and no waste generates, Ecological, environmental protective.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form Limitation, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.

Claims (6)

1. inoculation, bacteria, long mushroom and waste on a kind of ecological circulation cultural method of elegant precious mushroom, including bacterium bag production, bacterium bag Collection recycles step, it is characterised in that: the bacterium bag is made as adding water to be adjusted to water content 60-65% compost, so It is fitted into polypropylene single port polybag, sterilizes afterwards, it is cooling;The compost is mixed by following raw material by weight: mulberry 100 parts of powder of branch, 15-20 parts of pecan shell crushed material, 10-15 parts of Chinese chestnut bud husk crushed material, 5-10 parts of corn flour, organic-inorganic 20-30 parts of nutrition complex, 1-2 parts of lime;
The organic-inorganic nutrition complex is mixed by following raw material components by weight: 100 parts of wormcast, Algemin It minces 30-50 parts, 30-50 parts of fly maggot excrement, 20-30 parts of straw powder, 10-20 parts of bamboo vinegar, 10-20 parts of pine needle meal, molasses 10-20 Part;
During long mushroom, insect prevention activation processing is carried out to bacterium bag before fruiting again after fruiting of bacterium bag, insect prevention is activated Are as follows: the fruiting end at the top of bacterium bag is submerged into vertically downward into insect prevention activating solution and is impregnated 10-12 hours, the fruiting at the top of bacterium bag Hold the submergence height in insect prevention activating solution in 2-3cm;Bacterium bag fruiting 7-8 times in total;
Waste gathering is recycling as collecting discarded bacterium bag and the discarded mushroom handle mushroom base of a fruit enters methane-generating pit production biogas, production The fuel of heat source needed for biogas sterilizes as bacterium bag, the biogas slurry and biogas residue generated in methane-generating pit are used for mulberry tree kind as organic fertilizer It plants;
The proportion of the insect prevention activating solution each component by weight is as follows: 1000 parts of water, 2-4 parts of potassium sulfate, and ammonium phosphate 3-6 Part, 10-20 parts of bamboo vinegar, 20-30 parts of humic acid, 1-3 parts of inducer, 1-3 parts of promotor, 3-5 parts of vitamin C, Sargassum horneri extracts 15-25 parts of object.
2. a kind of ecological circulation cultural method of elegant precious mushroom according to claim 1, it is characterised in that: the organic-inorganic Nutrition complex the preparation method is as follows:
(1) wormcast, bulk kelp crushed material, fly maggot excrement, straw powder, bamboo vinegar, pine needle meal and molasses are uniformly mixed mixed Object is closed, into mixture plus water is adjusted to water content and obtains fermentation substrate for 60-65%;
(2) composite bacteria liquid is added into fermentation substrate to be uniformly mixed, composite bacteria liquid dosage is mixture weight in step (1) 5-10%, build heap fermentation after fermentation material, build heap fermentation environment temperature control at 20-30 DEG C, fermentation time 7-14 days;
(3) particle will be made after fermentation material drying, then particle is heat-treated, obtains organic-inorganic nutrition complex, institute after cooling Heat treatment is stated to be warming up to 180-200 DEG C with the heating rate of 2-5 DEG C/min, keeps 50-60min.
3. a kind of ecological circulation cultural method of elegant precious mushroom according to claim 1 or 2, it is characterised in that: the stalk Powder crushes after being mixed by corn stover, wheat stalk, soybean stalk, rape stalk, rice straw according to 1:1:1:1:1 weight ratio It forms.
4. a kind of ecological circulation cultural method of elegant precious mushroom according to claim 2, it is characterised in that: composite bacteria liquid is by EM Bacterium solution is mixed with Bacillus subtillis liquid according to the volume ratio of 1:0.5-0.6, viable count >=100,000,000/milliliter of EM bacterium solution, The viable count of Bacillus subtillis liquid >=100,000,000/milliliter.
5. a kind of ecological circulation cultural method of elegant precious mushroom according to claim 1, it is characterised in that: the Sargassum horneri extracts Object the preparation method is as follows: by Sargassum horneri crushed after being dried, solid-liquid ratio by crushed material and 85% ethyl alcohol according to 1g:15-20mL, In 40-45 DEG C is stirred 3-5 hours, and supernatant is collected in centrifugation, and the 10-15% of vacuum-concentrcted to original volume obtains concentrate, Concentrate ethyl acetate extracts 3-5 times, and last evaporated under reduced pressure removes ethyl acetate and obtains Sargassum horneri extract.
6. a kind of ecological circulation cultural method of elegant precious mushroom according to claim 1, it is characterised in that: the inducer is Diazosulfide, the promotor are oleic acid.
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CN104620858A (en) * 2015-03-12 2015-05-20 象州县科学技术局 Method for planting pleurotus geesteranus by utilizing mulberry twigs
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