CN109221805A - A kind of Tilapia mossambica efficient compound feed and preparation method thereof - Google Patents

A kind of Tilapia mossambica efficient compound feed and preparation method thereof Download PDF

Info

Publication number
CN109221805A
CN109221805A CN201811093605.4A CN201811093605A CN109221805A CN 109221805 A CN109221805 A CN 109221805A CN 201811093605 A CN201811093605 A CN 201811093605A CN 109221805 A CN109221805 A CN 109221805A
Authority
CN
China
Prior art keywords
raw material
enzyme
tilapia mossambica
compound feed
efficient compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811093605.4A
Other languages
Chinese (zh)
Inventor
何秋宏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201811093605.4A priority Critical patent/CN109221805A/en
Publication of CN109221805A publication Critical patent/CN109221805A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/132—Heterocyclic compounds containing only one nitrogen as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The present invention provides a kind of Tilapia mossambica efficient compound feed, belong to Aquatic Feed Processing technical field, it include protein sources raw material in the feed, fat source raw material, sugar source raw material, auxiliary material and coating raw material, further include algae nutrient, contain sodium benzoate and dehydroactic acid sodium in above-mentioned algae nutrient preparation homogeneous agent.The present invention also provides a kind of preparation methods of Tilapia mossambica efficient compound feed, mainly seaweed extracts algae nutrient through pickling, enzymatic hydrolysis, ultrasonic vibration, extruding after mixing again with protein sources raw material, sugar source raw material and auxiliary material is finally coated with fat source raw material and coating raw material to obtain the final product.Contain water soluble algae nutrient in a kind of Tilapia mossambica efficient compound feed provided by the invention, disintegration rate and dissolve-loss ratio are low in water, are disintegrated quickly in gastric juice, have high stability and high digestibility；The preparation method of the efficient compound feed has the advantages that high extraction efficiency, with short production cycle, raw material availability and yield are high, biological enzyme supported quantity is high.

Description

A kind of Tilapia mossambica efficient compound feed and preparation method thereof
Technical field
The invention belongs to Aquatic Feed Processing technical fields, and in particular to a kind of Tilapia mossambica efficient compound feed and its system Preparation Method.
Background technique
Tilapia mossambica has the characteristics that growth is fast, reproductive capacity is strong, feeding habits are miscellaneous, premunition is strong, is suitable for fresh water or salt-fresh water Pond, net cage, flowing water pond high density intensive culture, and Tilapia mossambica dressing percentage is high, and moderate product price is disappeared both at home and abroad The welcome of the person of expense, thus become by FAO (Food and Agriculture Organization of the United Nation) (FAO) recommend to world-wide deployment cultivate high-quality fish and I State dominates one of aquaculture kind, while China is also the maximum Tilapia mossambica exported country in the whole world.2014, global Tilapia mossambica produced Amount reaches 5,450,000 tons, wherein Chinese Rofe fish crop is 169.8 ten thousand tons.With the fast development of Tilapia mossambica aquaculture, to feed The demand of protein sources is continuously increased.Currently, high-vegetable-plant feed in Rofe fish culture using more and more extensive.But it is high Plant protein fodder contain it is some lose flavor substance and anti-nutritional factors, cause palatability is poor, to digest and absorb rate low to ask Topic, palatability difference directly result in aquatic livestock and ingest not positive or food refusal, influence the growth and physiological function of Tilapia mossambica, and feed Overlong time in water, had both caused the waste of feed, while also resulting in the pollution of breeding water body, how to pass through what addition was suitable for Feeding stimulants improve Tilapia mossambica ingest and growth performance, and inquire into its mechanism of action, have in Rofe fish culture important Theory significance and practical application value.
Insect is maximum guiding principle in the world, there is more than 200 ten thousand kinds, accounts for 2/3 or more of world animal sum, and insect has food Object is single, reproduction speed is fast, quantity is more, is easy to cultivate, at low cost, resourceful, the advantages that being easily managed, and belongs to sustainable benefit Living resources are a kind of very potential new protein sources for needing deep development.Protein contained by certain insects, Amino acid, essential fatty acid, vitamin, minerals etc. or its mutually make up, be able to satisfy the needs of aquatic economic animals, can Become the forage protein source of aquatic economic animals to substitute the fish meal of high price.Seaweed is more rich in protein, dietary fiber, seaweed Sugar, mannitol, high unsaturated fatty acid, amino acid, vitamin, more halogen multi-diterpene quasi-compounds, phytosterin compound and potassium, iron, The microelements such as calcium, phosphorus, iodine, selenium, cobalt and a variety of active skull cap components are a kind of high protein, low heat value, high physiological activity The raw material of natural ideal nutritional food, but the main nutrient composition of seaweed is to be combined in the form of insoluble with algae component , therefore nutritional ingredient is generally difficult to digest and assimilate.
The prior art such as Chinese invention patent CN102701866B disclose a kind of fermentation liquid seaweed extract material synergist and its Production method and purposes, wherein using natural seaweed as raw material, through microbial fermentation, then taken with alkali carries, the processes such as filters pressing produce sea Algae extracting solution, then adds chelated microelements, obtains fermentation alga liquid fertilizer synergist, organic molecule in seaweed extracted liquor Amount is smaller, and solid content is higher, is able to maintain lower viscosity and good mobility, and effective component extraction is more complete, but It is to be extracted by multiple physical and chemical method, the dissolution such as algin, mannitol, water soluble vitamin, minerals in seaweed It is lost, loses the active material in alginic cell significantly.
Summary of the invention
One of the objects of the present invention is to provide one kind containing water soluble algae nutrient, dissolve-loss ratio is low, is disintegrated in water Rate is low, Tilapia mossambica efficient compound feed quick, that stability is high, digestibility is high is disintegrated in gastric juice.
The second object of the present invention be to provide a kind of high extraction efficiency, with short production cycle, raw material availability and yield it is high, The preparation method of the high Tilapia mossambica efficient compound feed of biological enzyme supported quantity.
The technical solution that the present invention is taken to achieve the above object are as follows:
A kind of Tilapia mossambica efficient compound feed, including protein sources raw material, fat source raw material, sugar source raw material and auxiliary material, should Further include algae nutrient in feed, contains sodium benzoate and dehydroactic acid sodium in above-mentioned algae nutrient preparation homogeneous agent. Algae nutrient is added in the feed, wherein remaining sea containing components such as amino acid, polypeptide, polysaccharide, algin, algae oligosaccharides The effective component of algae whole, and these algae nutrients be it is water-soluble, fish are easy to absorb, and digestive utilization ratio is high, can promote The growth performance of fish improves meat quality.
As a kind of preferred embodiment of the invention, contains sodium benzoate in algae nutrient preparation homogeneous agent and take off Homogeneous agent is added in hydroacetic acid sodium during ultrasonic vibration slurrying, and homogeneous agent penetrates into rapidly thin under the assistance of ultrasonic wave Portion intracellular has an effect with the molecule not dissociated, inhibits absorption of the cell membrane to amino acid, increases the number of pores on cell membrane Amount, while obstinate bacterial spore can be thoroughly killed, the macromoleculars such as ionization degradation of polysaccharide, alkali storage form a large amount of small molecule actives Ingredient makes cell generate stronger collapse in Fast Stripping, is conducive to improve extraction efficiency, greatly shortens extraction time Meanwhile more active constituents and nutrition composition are obtained, improve raw material availability.
As a kind of preferred embodiment of the invention, seaweed is selected from Sargassum, asparagus, sargassum fusifome, chlorella, horse hair One or more of algae, spirulina.Seaweed is to can be carried out photosynthetic low autophyte in ocean, rich in protein, Vitamin, minerals, polysaccharide and omega-3 polyunsaturated fatty acids, seaweed are not only able to satisfy fish to the need of protein and energy It asks, moreover it is possible to enhance its immune function, increase economic efficiency.
As a kind of preferred embodiment of the invention, protein sources raw material includes animality and vegetable protein source, animal Property protein sources be fish meal, insect powder, vegetable protein source be dregs of beans, corn protein powder, peanut meal, rapeseed dregs, full fat rice bran, cotton At least two in the dregs of rice；Fat source raw material is at least one of soya-bean oil, corn oil, phosphatide, chicken fat, fish oil；Sugar source raw material is Alphalise starch.The parts by weight of above-mentioned several raw materials are as follows: 20~25 parts of animal protein source, 8~12 parts of vegetable protein source, fat 8~15 parts of source, 7~12 parts of sugar source.Above-mentioned insect powder be by the insects such as grub, fly maggot, yellow meal worm, locust be Raw material processing and At wherein containing higher crude protein content, amino acid ratio is complete, also rich in bioactivity such as chitin, natural antibacterial peptides Substance has effects that enhance immunity of organisms, resists poor environment stimulation, not only can be used as animal protein sources but also can replace For antibiotic.
As a kind of preferred embodiment of the invention, being coated with raw material includes that envelope forming agent and enzyme are coated with, wherein envelope Forming agent is selected from least one of guar gum, cellulose, sodium cellulose glycolate, chitosan, gelatin, agar.Enzyme coating and Envelope forming agent is coated with molding feed granules, enzyme can be made to be adsorbed onto particle surface, after fish, absorption Enzyme on feed granules surface is activated in gastric juice and is collapsed, and can greatly improve fish to the digestibility of feed, quilt is added Film forming agent can make enzyme more longlasting in the attachment of particle surface, and also making feed granules, molten mistake slows in water, reduce feed Dissolve-loss ratio and injected volume, reduce aquaculture cost.
As a kind of preferred embodiment of the invention, enzyme be coated with raw material be alpha-amylase, protease, in lipase extremely Few one kind.Fish can not secrete enough amylase and part protease when digesting feed, so that feed can not be disappeared completely Change and utilize, while these indigested ingredients will cause breeding water body eutrophication, therefore in feed with the excretion of fish External enzyme is added, digestive efficiency can be improved, reduces feed coefficient, reduces feed cost, improves breeding water body quality.
It is poly- including multi-vitamins, composite mineral salt, β-Portugal in auxiliary material as a kind of preferred embodiment of the invention Sugar, taurine, calcium dihydrogen phosphate, glycine betaine, nicotinate chromium and ethoxyquinoline.Each component and its parts by weight are such as in above-mentioned auxiliary material Under: 2~3 parts of multi-vitamins, 2~3 parts of composite mineral salt, 3~5 parts of beta glucan, 4~6 parts of taurine, calcium dihydrogen phosphate 1 ~3 parts, 2~4 parts of glycine betaine, 2~3 parts of nicotinate chromium and 2~3 parts of ethoxyquinoline.The addition of auxiliary material facilitate feed food calling, The gain effect of nutrition reinforcement, anti-metamorphic, resistance to storage etc..
Invention additionally discloses a kind of preparation methods of Tilapia mossambica efficient compound feed, specifically includes the following steps:
Step 1: after taking 9~13 parts of seaweed removal of impurities by weight, being cleaned with the dilute acid soln that pH is 2~4, and grind even Slurry, then enzyme enzymatic hydrolysis, after enzymolysis liquid is inactivated, filtering, filtrate and filter cake are spare；
Step 2: take step 1 gained filter cake to be added aqueous slkali and homogeneous agent, slurrying under the conditions of ultrasonic vibration, after the completion, Slurries are mixed with step 1 gained filtrate, it is dry to get algae nutrient；
Step 3: after mixing by protein sources raw material, sugar source raw material, algae nutrient and auxiliary material, steam modulation, extruding And it is dry, obtain swelling granular；
Step 4: after adding water that solution or dispersion liquid is made enzyme coating raw material, adding the stirring of envelope forming agent and coating is made Agent；
Step 5: after fat source raw material is sprayed on swelling granular, then being sprayed and be coated with emulsion, to get cooperation after cooling Feed.
As a kind of preferred embodiment of the invention, in step 1 dilute acid soln be sulfuric acid, nitric acid, in acetic acid at least One kind, additive amount are 3~10 times of seaweed weight, and scavenging period is 0.5~1h；Enzymatic hydrolysis with enzyme be cellulase, protease, At least one of lipase, pectase, by the vigor of enzyme be 50000 units in terms of, enzyme additive amount be seaweed weight 3~ 10%, enzymatic hydrolysis pH is 4~6, and temperature is 40~55 DEG C.Using weak acid scrubbing seaweed, while reaching washing effect, part The harmful heavy metal ions such as arsenic, lead, chromium are dispelled, and wherein inorganic iodine part is dispelled, but organic iodine is fully retained in frond In.
As a kind of preferred embodiment of the invention, aqueous slkali is ammonium hydroxide, potassium carbonate, sodium carbonate, hydroxide in step 2 At least one of calcium, additive amount are 4~6 times of filter cake weight；The additive amount of homogeneous agent be filter cake weight 0.13~ 0.25%, wherein the weight ratio of sodium benzoate and dehydroactic acid sodium is 3.5~3.8:2.
As a kind of preferred embodiment of the invention, the ultrasonic vibration mode in step 2 is sinusoidal state ultrasonic wave and change Frequency ultrasonic is aided with the processing mode of revolving speed concussion, revolving speed with the progress of-sine-frequency conversion-sine-frequency conversion alternating sequence For 150~200rpm, slurrying temperature is 85~95 DEG C, and the time is 1~2h；Sinusoidal state ultrasonic frequency is 32~36kHz, power For 200mW/cm2, the time is 10~20min；Variable-frequency ultrasound wave frequency rate is 36~42kHz, power 200mW/cm2, the time 20 ~30min.Seaweed is handled in a manner of ultrasonic wave extraction, promotes active material in seaweed using ultrasonic cavitation Fast Stripping, vibration amplitude can be increased using composite ultraphonic, increase mass transfer surfaces product, the maximum cavitation bubble of generation is bigger, And can be reduced dead angle caused by standing wave, and yield is improved, it is more abundanter than the nutritional ingredient in general ultrasonic extraction product.
As a kind of preferred embodiment of the invention, steam refining temperature is 95~105 DEG C in step 3, the time is 2~ 3min；Swelling temperature is 110~130 DEG C；Drying temperature is 80~90 DEG C, and the time is 10~15min.
As a kind of preferred embodiment of the invention, the amount of enzyme coating raw material is the 15 of swelling granular weight in step 4 ~20%, the amount of envelope forming agent is the 5~10% of swelling granular weight, and the concentration of enzyme solutions or suspension is 10~20%.
The invention has the benefit that
1) in feed of the present invention be added using composite ultraphonic wave extract algae nutrient, wherein containing amino acid, polypeptide, The components such as polysaccharide, algin, algae oligosaccharides, unsaturated fatty acid remain the nutrition effective component of seaweed, and algae nutrient is Water-soluble, fish are easy to absorb, and digestive utilization ratio is high, can promote the growth performance of fish, improve meat quality, moreover it is possible to enhance Its immune function；
2) feed granules are coated with using enzyme in the present invention, capable of making feed granules, molten mistake slows in water, moreover it is possible to significantly Fish are improved to the digestibility of feed, feed coefficient is reduced, reduces the dissolve-loss ratio and injected volume of feed, reduce cultivation at This, at the same mitigate because fish excretion caused by water eutrophication；
3) efficient compound feed nutrition full price provided by the invention, rationally, dissolve-loss ratio is low after dispensing, water resistance for proportion It is good, after fish, the fater disintegration in gastric juice, and facilitate the digestion and absorption of feed, promote mentioning for fish growth performance Height reduces aquaculture cost；
4) preparation method of efficient compound feed provided in the present invention is simple, easy to operate, and extraction efficiency is high, the production cycle Short, raw material availability and yield are high, and the supported quantity of made feed biological enzyme is high, and easy disintegrating, stability is not good in water.
Present invention employs above-mentioned technical proposals to provide a kind of Tilapia mossambica efficient compound feed and preparation method thereof, makes up The deficiencies in the prior art, reasonable design, easy operation.
Specific embodiment
Technical solution of the present invention is described in further detail below in conjunction with specific embodiment:
Embodiment 1:
A kind of Tilapia mossambica efficient compound feed, including protein sources raw material, fat source raw material, sugar source raw material and auxiliary material, should Further include algae nutrient in feed, contains sodium benzoate and dehydroactic acid sodium in above-mentioned algae nutrient preparation homogeneous agent. Algae nutrient is added in the feed, wherein remaining sea containing components such as amino acid, polypeptide, polysaccharide, algin, algae oligosaccharides The effective component of algae whole, and these algae nutrients be it is water-soluble, fish are easy to absorb, and digestive utilization ratio is high, can promote The growth performance of fish improves meat quality.
Contain sodium benzoate and dehydroactic acid sodium in algae nutrient preparation homogeneous agent, in the process of ultrasonic vibration slurrying Middle addition homogeneous agent, homogeneous agent penetrate into rapidly cell interior under the assistance of ultrasonic wave, make with the molecule not dissociated With absorption of the inhibition cell membrane to amino acid increases the hole quantity on cell membrane, while can thoroughly kill obstinate bacterium Spore, the macromoleculars such as ionization degradation of polysaccharide, alkali storage, forms a large amount of small molecule active ingredients, generates cell in Fast Stripping Stronger collapse is conducive to improve extraction efficiency, while greatly shortening extraction time, obtains more active constituents and battalion Component is supported, raw material availability is improved.
Seaweed is the equal amount of mixture of asparagus and sargassum fusifome.Seaweed is to can be carried out photosynthetic low autotrophy in ocean Plant, is rich in proteins,vitamins,minerals, polysaccharide and omega-3 polyunsaturated fatty acids, and seaweed is not only able to satisfy fish to egg The demand of white matter and energy, moreover it is possible to enhance its immune function, increase economic efficiency.
Protein sources raw material includes animality and vegetable protein source, and animal protein source is fish meal, insect powder, vegetalitas egg Bai Yuanwei dregs of beans, corn protein powder；Fat source raw material is corn oil；Sugar source raw material is alphalise starch.The weight of above-mentioned several raw materials Part is as follows: 5 parts of fish meal, 16 parts of insect powder, 4 parts of dregs of beans, 5 parts of corn protein powder, 8 parts of corn oil, and 8 parts of alphalise starch.Above-mentioned insect Powder is to be formed by insects such as grub, fly maggot, yellow meal worm, locusts for Raw material processing, wherein containing higher crude protein content, amino Acid proportion is complete, also rich in the bioactive substances such as chitin, natural antibacterial peptide, have enhancing immunity of organisms, resist it is bad The effect of environmental stimulus, animal protein sources were not only can be used as but also can be with substitute antibiotics.
Coating raw material includes envelope forming agent and enzyme coating, and wherein envelope forming agent is guar gum.Enzyme coating and envelope shape Molding feed granules are coated at agent, enzyme can be made to be adsorbed onto particle surface, after fish, be adsorbed on feed The enzyme of particle surface is activated in gastric juice and is collapsed, and can greatly improve fish to the digestibility of feed, envelope is added and is formed Agent can make enzyme more longlasting in the attachment of particle surface, and also making feed granules, molten mistake slows in water, reduce the molten mistake of feed Rate and injected volume reduce aquaculture cost.
It is alpha-amylase that enzyme, which is coated with raw material,.Fish can not secrete enough amylase and part albumen when digesting feed Enzyme, so that feed can not be digested utilization completely, while these indigested ingredients will cause cultivation water with the excretion of fish Body eutrophication, therefore external enzyme is added in feed, digestive efficiency can be improved, reduce feed coefficient, reduce feed cost, Improve breeding water body quality.
It include multi-vitamins, composite mineral salt, beta glucan, taurine, calcium dihydrogen phosphate, glycine betaine, cigarette in auxiliary material Sour chromium and ethoxyquinoline.Each component and its parts by weight are as follows in above-mentioned auxiliary material: 2 parts of multi-vitamins, 2 parts of composite mineral salt, 3 parts of beta glucan, 4 parts of taurine, 1.5 parts of calcium dihydrogen phosphate, 2 parts of glycine betaine, 2 parts of nicotinate chromium and 2 parts of ethoxyquinoline.Auxiliary material Addition facilitate feed food calling, nutrition reinforcement, anti-metamorphic, it is resistance in terms of gain effect.
A kind of preparation method of Tilapia mossambica efficient compound feed, specifically includes the following steps:
Step 1: after taking 9 parts of seaweed removal of impurities by weight, being cleaned with the dilute acid soln that pH is 3, and grind homogenate, then enzyme Enzymatic hydrolysis, after enzymolysis liquid is inactivated, filtering, filtrate and filter cake are spare；
Step 2: take step 1 gained filter cake to be added aqueous slkali and homogeneous agent, slurrying under the conditions of ultrasonic vibration, after the completion, Slurries are mixed with step 1 gained filtrate, it is dry to get algae nutrient；
Step 3: after mixing by protein sources raw material, sugar source raw material, algae nutrient and auxiliary material, steam modulation, extruding And it is dry, obtain swelling granular；
Step 4: after adding water that solution or dispersion liquid is made enzyme coating raw material, adding the stirring of envelope forming agent and coating is made Agent；
Step 5: after fat source raw material is sprayed on swelling granular, then being sprayed and be coated with emulsion, to get cooperation after cooling Feed.
Dilute acid soln is acetic acid in step 1, and additive amount is 8 times of seaweed weight, scavenging period 0.5h；Enzymatic hydrolysis uses enzyme For cellulase, be 50000 units by the vigor of enzyme in terms of, enzyme additive amount is the 5% of seaweed weight, and enzymatic hydrolysis pH is 4.5, and temperature is 45℃.Using weak acid scrubbing seaweed, while reaching washing effect, the harmful heavy metal ions such as part arsenic, lead, chromium are dispelled It removes, wherein inorganic iodine part is dispelled, but organic iodine is fully retained in frond.
Aqueous slkali is potassium carbonate in step 2, and additive amount is 6 times of filter cake weight；The additive amount of homogeneous agent is filter cake weight The 0.17% of amount, wherein the weight ratio of sodium benzoate and dehydroactic acid sodium is 3.5:2.
Ultrasonic vibration mode in step 2 is sinusoidal state ultrasonic wave and variable-frequency ultrasonic with-sine-frequency conversion-sine-frequency conversion Alternating sequence carry out, while being aided with the processing mode of revolving speed concussion, revolving speed 150rpm, slurrying temperature is 85 DEG C, and the time is 2h；Sinusoidal state ultrasonic frequency is 32kHz, power 200mW/cm2, time 15min；Variable-frequency ultrasound wave frequency rate is 36kHz, Power is 200mW/cm2, time 20min.Seaweed is handled in a manner of ultrasonic wave extraction, utilizes ultrasonic cavitation Promote the Fast Stripping of active material in seaweed, vibration amplitude can be increased using composite ultraphonic, increases mass transfer surfaces product, produce Raw maximum cavitation bubble is bigger, and can be reduced dead angle caused by standing wave, yield is improved, than the nutrition in general ultrasonic extraction product Ingredient is more abundant.
Steam refining temperature is 95 DEG C in step 3, time 3min；Swelling temperature is 115 DEG C；Drying temperature is 90 DEG C, Time is 10min.
The amount of enzyme coating raw material is the 16.5% of swelling granular weight in step 4, and the amount of envelope forming agent is swelling granular The concentration of the 7.5% of weight, enzyme solutions or suspension is 13.5%.
Embodiment 2:
A kind of Tilapia mossambica efficient compound feed, wherein protein sources raw material, fat source raw material, sugar source raw material component and its Parts by weight are as follows: 7 parts of fish meal, 17 parts of insect powder, 5 parts of corn protein powder, 5 parts of rapeseed dregs, 4 parts of soya-bean oil, 7 parts of phosphatide, alphalise starch 10 parts.
The component and its parts by weight of auxiliary material are as follows in above-mentioned feed: 2.5 parts of multi-vitamins, 2.5 parts of composite mineral salt, β- .5 parts of bextran 45,5 parts of taurine, 2.5 parts of calcium dihydrogen phosphate, 3.5 parts of glycine betaine, 3 parts of nicotinate chromium and 2.5 parts of ethoxyquinoline.
In the coating raw material of above-mentioned feed envelope forming agent be sodium cellulose glycolate, enzyme be coated with raw material be alpha-amylase, The equal amount of mixture of protease, lipase.
A kind of preparation method of Tilapia mossambica efficient compound feed, specifically includes the following steps:
Step 1: after taking 12 parts of seaweed removal of impurities by weight, cleaned with the dilute acid soln that pH is 4, and grind homogenate, then plus Enzyme enzymatic hydrolysis, after enzymolysis liquid is inactivated, filtering, filtrate and filter cake are spare；
Step 2: take step 1 gained filter cake to be added aqueous slkali and homogeneous agent, slurrying under the conditions of ultrasonic vibration, after the completion, Slurries are mixed with step 1 gained filtrate, it is dry to get algae nutrient；
Step 3: after mixing by protein sources raw material, sugar source raw material, algae nutrient and auxiliary material, steam modulation, extruding And it is dry, obtain swelling granular；
Step 4: after adding water that solution or dispersion liquid is made enzyme coating raw material, adding the stirring of envelope forming agent and coating is made Agent；
Step 5: after fat source raw material is sprayed on swelling granular, then being sprayed and be coated with emulsion, to get cooperation after cooling Feed.
Seaweed is asparagus, sargassum fusifome, spirulina equal amount of mixture in step 1；Dilute acid soln is nitric acid, and additive amount is 6 times of seaweed weight, scavenging period 0.75h；Enzymatic hydrolysis is cellulase, protease with enzyme, with the vigor of enzyme for 50000 units Meter, enzyme additive amount are the 7.5% of seaweed weight, and enzymatic hydrolysis pH is 5.5, and temperature is 48 DEG C.
Aqueous slkali is sodium carbonate in step 2, and additive amount is 4 times of filter cake weight；The additive amount of homogeneous agent is filter cake weight The 0.21% of amount, wherein the weight ratio of sodium benzoate and dehydroactic acid sodium is 3.7:2.
Ultrasonic vibration mode in step 2 is sinusoidal state ultrasonic wave and variable-frequency ultrasonic with-sine-frequency conversion-sine-frequency conversion Alternating sequence carry out, while being aided with the processing mode of revolving speed concussion, revolving speed 200rpm, slurrying temperature is 90 DEG C, and the time is 1h；Sinusoidal state ultrasonic frequency is 35kHz, power 200mW/cm2, time 20min；Variable-frequency ultrasound wave frequency rate is 40kHz, Power is 200mW/cm2, time 25min.
Steam refining temperature is 100 DEG C in step 3, time 2min；Swelling temperature is 125 DEG C；Drying temperature is 85 DEG C, Time is 13min.
The amount of enzyme coating raw material is the 18.7% of swelling granular weight in step 4, and the amount of envelope forming agent is swelling granular The concentration of the 8.6% of weight, enzyme solutions or suspension is 18.3%.
Embodiment 3:
The present embodiment further progress on the basis of embodiment 2 optimizes, wherein in the preparation for carrying out efficient compound feed In the process, step 4 is optimized, specific Optimization Steps are as follows: after adding water that solution or dispersion liquid is made enzyme coating raw material, then It is added immobilized dose and coating agent is made in the stirring of envelope forming agent, the amount of above-mentioned enzyme coating raw material is swelling granular weight 18.7%, the amount of envelope forming agent is the 8.6% of swelling granular weight, and the concentration of enzyme solutions or suspension is 18.3%, immobilized The additive amount of agent is that enzyme is coated with the 0.77% of raw material, the weight of Tert-butyl Methacrylate and p-Coumaric Acid methyl esters in immobilized dose Amount than being 1:1.5, immobilized dose can enzyme in reduction system, water, the solid-liquid between envelope forming agent, liquid liquid surface tension, promote life Object enzyme quickly and evenly disperses in system, and by intermiscibility better between grease during coating, so that individually The supported quantity of biological enzyme obviously rises on feed granules, can promote digestion and absorption of the fish to feed, in addition immobilized dose and envelope Mechanical shearing is utilized between forming agent, is capable of forming the multidimensional structure of gantry, wherein by biological enzyme package, to make biological enzyme It can only be attached on the outside of feed granules, and be unable to endosmosis and have an effect, to make feed nutrition when long-time saves It will not be lost, and keep high stability inside particle, further decrease the dissolve-loss ratio of feed disintegration rate in water and feed.
The present embodiment is optimized on the basis of embodiment 2, made mixed feed raw material and other preparation steps It is consistent with embodiment 2, mixed feed is made.
Comparative example:
A kind of preparation method of Tilapia mossambica efficient compound feed, wherein specific step is as follows for step 2: taking step 1 institute It obtains filter cake addition aqueous slkali and homogeneous agent, slurrying under the conditions of ultrasonic vibration after the completion mixes slurries and step 1 gained filtrate It closes, dry to get algae nutrient, above-mentioned aqueous slkali is sodium carbonate, and additive amount is 4 times of filter cake weight；Homogeneous agent adds Dosage is the 0% of filter cake weight, i.e., is not added with homogeneous agent in the step.
This comparative example is that test is compared on the basis of embodiment 2, made mixed feed raw material and other preparations Step and embodiment 2 are consistent, and efficient compound feed is made.
Embodiment 4:
Tilapia mossambica efficient compound feed feeding experiment
In certain freshwater aquiculture production base, using the made mixed feed of Examples 1 to 3 as test group 1~3, comparative example institute Mixed feed processed as a control group, using certain commercially available mixed feed as blank group, randomly chooses 10 pools mouthful, be divided into 2 groups it is parallel, put After identical quantity, original body mass are supported without the Tilapia mossambica fry of significant difference, feed day 3~4 times, daily ration of feeding is identical, during which not Administration of antibiotics.Reinforce process management in breeding process, after two weeks, sample analysis is acquired, as a result such as the following table 1.
Influence of 1 efficient compound feed of table to Growth Op Tilapia performance
Test group 1 Test group 2 Test group 3 Control group Blank group
First counterpoise/g 3.34 3.30 3.38 3.35 3.34
Last counterpoise/g 28.87 28.95 29.69 27.73 25.64
Specific growth rate/% 3.81 3.87 3.92 3.78 3.61
Feed dissolve-loss ratio/% 10.9 10.5 7.5 10.3 18.8
Protein efficiency ratio, PER 2.08 2.06 2.07 2.10 1.93
Feed efficiency/% 72.39 73.66 75.24 72.66 68.62
As shown in Table 1, the dissolve-loss ratio of blank group feed dissolve-loss ratio highest, test group 1,2 and control group is not much different, also bright It is aobvious to be better than blank group, and test group 3 is due to being optimized, feed dissolve-loss ratio further decreases, and illustrates in test group 3 immobilized dose Addition have remarkable result to the reduction of the dissolve-loss ratio of feed；Since feed dissolve-loss ratio reduces, the feed of Tilapia mossambica intake increases, and raises Nutritional ingredient in material can be fully absorbed utilization, so test group and the feed efficiency of control group, last counterpoise are bright compared with blank group It is aobvious to increase, illustrate that made feed has significant gain effect to the growth performance of Tilapia mossambica in embodiment.
Embodiment 5:
Tilapia mossambica efficient compound feed slaking test
The preparation of simulated gastric fluid: 10% hydrochloric acid is added into water, is prepared into solution (the fish Gastric pH that pH reaches 3.0 It is 2.6~3.8).
Slaking test: respectively using embodiment 2, the made feed of embodiment 3 and certain commercially available mixed feed as test group 1,2, 3, test is respectively put into 100ML triangular flask with feed granules, water and simulated gastric fluid 80ML are respectively provided in triangular flask, then It is placed on 150 beats/min of shaking table and is tested, as a result such as the following table 2.
The slaking test of 2 mixed feed of table
Time completely soluble in water The time being completely dissolved in simulated gastric fluid
Test group 1 3~3.5h 1.5~2h
Test group 2 4.5~5h 1~1.5h
Test group 3 2~2.5h 2.5~3h
As shown in Table 2, the feed granules of test group 3 are easier to be disintegrated in water, but dissolve relatively slowly in gastric juice, lead It causes under identical injected volume, it is less that fish take in forage volume, and digests and assimilates and be not enough；Test group 1,2 is able to maintain in water Higher integrity and stability is disintegrated comparatively fast in gastric juice, takes the photograph conducive to fish due to being coated with enzyme coating outside feed Nutritional ingredient is digested and assimilated after food；Test group 2 is disintegrated slowly in water compared with test group 1, and fast in simulated gastric fluid, is due to test Group 2 it is optimized after, immobilized dose is attached to biological enzyme outside feed, can not endosmosis effect, to be able to maintain feeding in water The stability of material after biological enzyme activates in simulated gastric fluid, acts on rapidly, thus can fater disintegration in simulated gastric fluid.
The preparation method of Tilapia mossambica efficient compound feed in the present invention, cleaning dilute acid soln not only includes in step 1 At least one of sulfuric acid, nitric acid, acetic acid can also include sulfuric acid, nitric acid, acetic acid, phosphoric acid, hydrochloric acid；Alkali soluble used in step 2 Liquid not only includes at least one of ammonium hydroxide, potassium carbonate, sodium carbonate, calcium hydroxide, can also include sodium hydroxide, hydroxide Potassium；In step 4 envelope forming agent not only include guar gum, cellulose, sodium cellulose glycolate, chitosan, gelatin, in agar At least one can also include natural rubber, alginates, chitosan salt, Sodium Polyacrylate, poly- methylurea, ligninsulfonate, angle Pitch at least one of dish glue；Enzyme coating raw material not only includes alpha-amylase, protease, at least one in lipase in step 4 Kind, it can also include zytase, 1,4 beta-glucanase, 'beta '-mannase, beta amylase, hemicellulase, cellulase, fruit The carbohydrate-splitting enzymes such as glue enzyme, glucoamylase, lactase, zytase, chitosan enzyme can also decompose phosphorus including phytase etc. Enzyme can also include alkali protease, acid protease, neutral proteinase, plant rennet, bromelain, Papain The proteases such as enzyme, can be used more than one.
The prior art of routine techniques dawn known to those skilled in the art in above-described embodiment, therefore herein no longer in detail It repeats.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.

Claims (10)

1. a kind of Tilapia mossambica efficient compound feed, including protein sources raw material, fat source raw material, sugar source raw material, auxiliary material and coating Raw material, it is characterised in that: further include algae nutrient in feed；Contain benzoic acid in the algae nutrient preparation homogeneous agent Sodium and dehydroactic acid sodium.
2. a kind of Tilapia mossambica efficient compound feed according to claim 1, it is characterised in that: the seaweed is selected from extra large wormwood artemisia At least one of son, asparagus, sargassum fusifome, chlorella, sargassum, spirulina.
3. a kind of Tilapia mossambica efficient compound feed according to claim 1, it is characterised in that: the coating raw material includes Envelope forming agent and enzyme coating；The envelope forming agent is selected from guar gum, cellulose, sodium cellulose glycolate, chitosan, bright At least one of glue, agar.
4. a kind of Tilapia mossambica efficient compound feed according to claim 3, it is characterised in that: the enzyme is coated with raw material and is At least one of alpha-amylase, protease, lipase.
5. a kind of Tilapia mossambica efficient compound feed according to claim 1, it is characterised in that: include multiple in the auxiliary material Close vitamin, composite mineral salt, beta glucan, taurine, calcium dihydrogen phosphate, glycine betaine, nicotinate chromium and ethoxyquinoline.
6. a kind of described in any item preparation methods of Tilapia mossambica efficient compound feed of claim 1 ~ 5, it is characterised in that: packet Include following steps:
Step 1: after taking seaweed to clean, being cleaned with the dilute acid soln that pH is 2 ~ 4, and grind homogenate, then enzyme enzymatic hydrolysis, by enzymolysis liquid After inactivation, filtering, filtrate and filter cake are spare；
Step 2: taking step 1 gained filter cake that aqueous slkali and homogeneous agent, slurrying under the conditions of ultrasonic vibration is added will starch after the completion Liquid is mixed with step 1 gained filtrate, dry to get algae nutrient；
Step 3: after mixing by protein sources raw material, sugar source raw material, algae nutrient and auxiliary material, steam modulation, extruding is simultaneously done It is dry, obtain swelling granular；
Step 4: after adding water that solution or dispersion liquid is made enzyme coating raw material, adding the stirring of envelope forming agent and coating agent is made；
Step 5: after fat source raw material is sprayed on swelling granular, then being sprayed and be coated with coating agent, raised after cooling to get cooperation Material.
7. a kind of preparation method of Tilapia mossambica efficient compound feed according to claim 6, it is characterised in that: the step The additive amount of dilute acid soln is 3~10 times of seaweed weight in rapid 1, and scavenging period is 0.5 ~ 1h；Enzymatic hydrolysis with enzyme be cellulase, At least one of protease, lipase, pectase, by the vigor of enzyme be 50000 units in terms of, enzyme additive amount be seaweed weight 3 ~ 10%, enzymatic hydrolysis pH be 4 ~ 6, temperature be 40 ~ 55 DEG C.
8. a kind of preparation method of Tilapia mossambica efficient compound feed according to claim 6, it is characterised in that: the step The additive amount of aqueous slkali is 4 ~ 6 times of filter cake weight in rapid 2, and the additive amount of homogeneous agent is the 0.13 ~ 0.25% of filter cake weight, The weight ratio of middle sodium benzoate and dehydroactic acid sodium is 3.5 ~ 3.8:2.
9. a kind of preparation method of Tilapia mossambica efficient compound feed according to claim 6, it is characterised in that: the step Ultrasonic vibration mode in rapid 2 is sinusoidal state ultrasonic wave and variable-frequency ultrasonic with-sine-frequency conversion-sine-frequency conversion alternating sequence Carry out, at the same be aided with revolving speed concussion processing mode, revolving speed be 150 ~ 200rpm, slurrying temperature be 85 ~ 95 DEG C, the time be 1 ~ 2h；The sine state ultrasonic frequency is 32 ~ 36kHz, power 200mW/cm2, the time is 10 ~ 20min；The variable-frequency ultrasound Wave frequency rate is 36 ~ 42kHz, power 200mW/cm2, the time is 20 ~ 30min.
10. a kind of preparation method of Tilapia mossambica efficient compound feed according to claim 6, it is characterised in that: described The amount of enzyme coating raw material is the 15 ~ 20% of swelling granular weight in step 4, the amount of envelope forming agent be swelling granular weight 5 ~ 10%, the concentration of enzyme solutions or suspension is 10 ~ 20%.
CN201811093605.4A 2018-09-19 2018-09-19 A kind of Tilapia mossambica efficient compound feed and preparation method thereof Pending CN109221805A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811093605.4A CN109221805A (en) 2018-09-19 2018-09-19 A kind of Tilapia mossambica efficient compound feed and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811093605.4A CN109221805A (en) 2018-09-19 2018-09-19 A kind of Tilapia mossambica efficient compound feed and preparation method thereof

Publications (1)

Publication Number Publication Date
CN109221805A true CN109221805A (en) 2019-01-18

Family

ID=65058262

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811093605.4A Pending CN109221805A (en) 2018-09-19 2018-09-19 A kind of Tilapia mossambica efficient compound feed and preparation method thereof

Country Status (1)

Country Link
CN (1) CN109221805A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111493009A (en) * 2020-06-16 2020-08-07 通威股份有限公司 Greenhouse breeding method for tilapia mossambica by using geothermal water
CN114698749A (en) * 2022-03-23 2022-07-05 福建省农业科学院生物技术研究所 Functional aquatic product puffed pellet feed and preparation method thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102488253A (en) * 2011-12-28 2012-06-13 青岛明月海洋科技有限公司 Preparation method of active seaweed extract
CN102573515A (en) * 2009-07-10 2012-07-11 大峰Ls株式会社 Pellet feed for fish farming having excellent dissolution and digestion efficiency, and method for preparing same
CN102813089A (en) * 2012-08-23 2012-12-12 中国水产科学研究院长江水产研究所 High-effective compound feed for late stage of tilapia cultivation and preparation method thereof
CN103960524A (en) * 2014-04-11 2014-08-06 汕头大学 Tilapia mossambica feed and preparation method thereof
CN104489326A (en) * 2014-12-09 2015-04-08 汕头大学 Fish feed enzyme preparation and preparation and addition methods thereof as well as fish feed
CN107254014A (en) * 2017-07-03 2017-10-17 陕西师范大学 A kind of complex solidifying enzyme carrier material and its preparation method and application
CN107410442A (en) * 2017-05-15 2017-12-01 重庆凰巢实业有限公司 A kind of meat product antistaling disinfectant and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102573515A (en) * 2009-07-10 2012-07-11 大峰Ls株式会社 Pellet feed for fish farming having excellent dissolution and digestion efficiency, and method for preparing same
CN102488253A (en) * 2011-12-28 2012-06-13 青岛明月海洋科技有限公司 Preparation method of active seaweed extract
CN102813089A (en) * 2012-08-23 2012-12-12 中国水产科学研究院长江水产研究所 High-effective compound feed for late stage of tilapia cultivation and preparation method thereof
CN103960524A (en) * 2014-04-11 2014-08-06 汕头大学 Tilapia mossambica feed and preparation method thereof
CN104489326A (en) * 2014-12-09 2015-04-08 汕头大学 Fish feed enzyme preparation and preparation and addition methods thereof as well as fish feed
CN107410442A (en) * 2017-05-15 2017-12-01 重庆凰巢实业有限公司 A kind of meat product antistaling disinfectant and preparation method thereof
CN107254014A (en) * 2017-07-03 2017-10-17 陕西师范大学 A kind of complex solidifying enzyme carrier material and its preparation method and application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111493009A (en) * 2020-06-16 2020-08-07 通威股份有限公司 Greenhouse breeding method for tilapia mossambica by using geothermal water
CN114698749A (en) * 2022-03-23 2022-07-05 福建省农业科学院生物技术研究所 Functional aquatic product puffed pellet feed and preparation method thereof

Similar Documents

Publication Publication Date Title
CN104041717B (en) A kind of vinasse laying cycle of laying hens special feed processed and preparation method thereof
WO2016029647A1 (en) Active seaweed feed additive and preparation method therefor
CN102919526B (en) Bamboo powder fermentation feed and preparation method thereof
CN102960549B (en) Feeding complex enzyme preparation by using Chinese medicaments as carriers and diluting agents, as well as preparation method thereof
CN103859155A (en) Refining method for probiotics-containing cassava feed
CN103667220B (en) Neutral protease-containing growing pig dedicated enzyme and preparation method thereof
CN107311707A (en) A kind of preparation method of selenium-enriched earthworm organic foliar fertilizer
CN102987077A (en) Preparation method of seaweed fermented feed
CN104351557A (en) Fish fodder and preparation method thereof
CN103829042A (en) Production method of multi-vitamin active cassava protein feed
CN109221805A (en) A kind of Tilapia mossambica efficient compound feed and preparation method thereof
CN103859169A (en) Feed compound enzyme containing neutral protease and preparation method thereof
CN103859148A (en) Production technology of bacillus subtilis fermented soybean meal
CN103540554B (en) Post-sprayed micro-ecological preparation for expanded feeds, and preparation method and application of preparation
CN105394335A (en) Calcium supplement type diarrhea prevention pig feed and preparation method thereof
CN112195213A (en) Method for extracting protein powder by cultivating hermetia illucens with food waste solids
CN103621790A (en) Composite earthworm enzymatic hydrolysate feed additive and preparation method thereof
CN106721588A (en) It is a kind of to strengthen disease-resistant and anti-stress ability feather hydrolysis powder feed of Macrobrachium rosenbergii and preparation method thereof
CN103740683B (en) A kind of Wheat ration enzyme containing neutral protease and preparation method thereof
CN103519028B (en) A kind of broiler breeding feed and method for breeding thereof
CN103113493B (en) A kind of technique utilizing microecological microbial agent solid state fermentation to transform shrimp crab abandoned biomass cleanly production chitin
CN105394378A (en) Calcium supplement type fattening large pig feed and preparation method thereof
CN109198164A (en) A kind of soft-shelled turtle feed and preparation method thereof
CN105746886B (en) A method of compound green feed additive for promoting growth is prepared using aquatic product protein and Chinese herbal medicine fermentation
CN105707473B (en) A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20190118