CN112195213A - Method for extracting protein powder by cultivating hermetia illucens with food waste solids - Google Patents
Method for extracting protein powder by cultivating hermetia illucens with food waste solids Download PDFInfo
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- CN112195213A CN112195213A CN201910617311.5A CN201910617311A CN112195213A CN 112195213 A CN112195213 A CN 112195213A CN 201910617311 A CN201910617311 A CN 201910617311A CN 112195213 A CN112195213 A CN 112195213A
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Abstract
The invention provides a method for extracting protein powder by using food waste solids to breed hermetia illucens, relates to the field of biological industry, and particularly relates to a method for extracting protein powder by using food waste solids to breed hermetia illucens. The high-content gadfly pulp enzyme protein powder extracted from hermetia illucens is prepared from the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 550-650 parts of anhydrous ether, 900-1000 parts of deionized water, 7-10 parts of sodium hydroxide, 0.4-0.7 part of trypsin, 0.3-0.5 part of chymotrypsin and 2-3 parts of activated carbon. Firstly, carrying out ultraviolet sterilization and drying on the black water , crushing the black water into fine powder of 200 meshes, and carrying out degreasing on the fine powder: adding deionized water into the defatted powder, adjusting pH to 8 with sodium hydroxide, heating to a certain degree, soaking for a period of time, and adding 0.5% trypsin chymotrypsin for double-enzyme hydrolysis; then adding activated carbon, stirring at a low speed for 2 hours, filtering, decoloring and finally carrying out spray drying to obtain the high-content Tabanus pulp enzyme protein powder.
Description
The invention relates to the field of biochemical engineering. A method for extracting protein powder from hermetia illucens cultured by using food waste solids.
Background
The sources of the food wastes are mainly restaurants, dining halls, families and other places. Various organic substances contained in the pig feed are extremely easy to corrode in summer to cause pollution, and particularly once pigs fed by food waste and refined swill oil flow to the market, the pig feed seriously harms the health of people. At present, a large amount of food waste becomes one of main sources of urban pollution, and how to scientifically treat food waste in a harmless way becomes a difficult problem which puzzles urban management and construction. The method solves the problems of wastes and residues in the catering industry in urban development management, changes waste resources into renewable resources, and is a requirement for social civilization progress and sustainable development. The black soldier fly is a saprophytic soldier fly, larvae of the black soldier fly are an important part of a natural clastic food chain, food waste, animal excrement, animal and plant corpses, rotten fruits and vegetables and other waste are eaten, and the kitchen waste can be treated by utilizing the characteristics that the black soldier fly prefers to be eaten and the food intake is large. The excrement discharged by the black soldier fly larvae can be used as organic fertilizer, so that the environment cannot be polluted.
The black soldier flies are out of the dirty place and are placed in countless pathogenic bacteria, but can be safely and carelessly infected by the pathogenic bacteria. The vitality of the black soldier fly larvae is very strong, the rancid environment unbearable by other animals is a park, due to the excellent immune function, and the feeding of the black soldier fly generally does not worry about preventing diseases, so that the disease cost can be greatly saved.
In 2013, 10 months, food and agricultural organizations of the United nations used hermetia illucens as the most main protein feed to replace insects in the edible insect report. The hermetia illucens germplasm resources of China are rich, and more than 90 percent of the hermetia illucens germplasm resources are distributed in China, south China and southwest China. The hermetia illucens has the characteristics of large biomass, high content of insect body resources and the like, is rich in 17 amino acids, wherein saturated fatty acids mainly comprise lauric acid and palmitic acid, unsaturated fatty acids mainly comprise oleic acid, the protein of the hermetia illucens larvae cultured by directly utilizing fresh pig manure can reach 40.6%, and the total nutrient content of the insect sand is 8.76%, so that the national organic fertilizer standard can be reached.
And (3) recovering the kitchen waste, performing solid-liquid separation by using a solid-liquid separator, crushing and deodorizing the solid part, adding lactic acid bacteria, and feeding hermetia illucens larvae. The hermetia illucens larvae are not sensitive to salt, spices, food toxins and other chemical additives. Can be eaten all day long under the condition of sufficient food. According to the introduction, the hermetia illucens uses kitchen waste as feed, 2 kilograms of kitchen waste can be eaten by each hermetia illucens in a period of 72 hours, nutrients in the hermetia illucens can be effectively converted to hermetia illucens larvae, two useful things can be produced, one is high-quality insect protein, and the other is good organic fertilizer. In addition, the black soldier fly has very high oil content and can be used as aviation oil, a cosmetic raw material and the like.
Besides the application in the breeding direction and the fields of energy sources and the like, the hermetia illucens can also be applied in the fields of nutrition, health care, medicines and the like, the adipic acid in the larva extract has an antibacterial function, part of the extract also has the function of inhibiting the activity of bacteria such as gonococcus and the like, the hermetia illucens become important research objects of antibacterial peptide medicines, and the market profit of the high-end products is considerable.
The black soldier fly is regarded as a new resource, is honored as the king of insect bait, and is regarded as the third major industrial insect following silkworms and bees. According to the introduction of experts of the university of agriculture in Huazhong: about 2 tons of hermetia illucens can eat 1500 more tons of kitchen waste in one year. Its larva can be used as live feed for feeding chicken, duck, bullfrog and fish. The small insects can extract the gadfly pulp enzyme protein powder with high added value and can also become an environment-friendly guardian.
The life cycle of the hermetia illucens is only 40 days, and the hermetia illucens can live in an environment with the temperature of 25-35 ℃ and the humidity of 70% through 4 stages of eggs, larvae, pupae and adults. About 5 days after the soldier flies are hatched into larvae from eggs, the period is the strongest period for treating kitchen waste and livestock excrement. When the larva grows for 15 days, pupation is carried out, and mating and oviposition are carried out after pupation imagoes are broken, so that 800 to 1000 eggs can be produced by one stratiomyiid at one time. The black soldier fly larvae gradually turn black after 10 to 12 days, which is an indication that pupation is needed, and the feeding is stopped immediately. The larvae at this stage are the baby in their eyes because they are rich in crude protein, fat, calcium, phosphorus, etc., and can be dried to make a superior feed for poultry, livestock, and fish.
The black soldier flies have high propagation speed, and a pair of black soldier flies can propagate 3000 hundred million maggots in six months, and can accumulate 750 tons of pure protein. Is incomparable with other methods for producing animal protein so far.
The black soldier fly can not only extract protein powder and develop high-grade nutritional food, but also can obtain various biochemical products such as fat, antibiotics, agglutinin and the like simultaneously in the production process. The antibacterial protein can kill all fungal microorganisms and has strong bactericidal effect. The hermetia illucens pupa shells are the best raw materials for extracting chitin, and the chitin is regarded as the sixth vital element necessary for human bodies except sugar, protein fat, vitamins and mineral substances. The black soldier fly is simple and easy to produce, does not need any epidemic prevention measure or a modern factory building for producing the black soldier fly, and can be produced in a large scale by preserving heat, supplying food and preventing escape under civil hydropower equipment. Does not produce toxic substances and pollute the environment, and the black soldier fly can be called as an excellent chemical engineer.
Ten advantages of gadfly pulp enzyme protein powder extracted from hermetia illucens
1. Can obviously enhance the immune function of body fluid and cells, improve the disease resistance and reduce the culture risk.
2. Can enhance the synthesis and utilization of protein in vivo, improve the production performance of livestock and poultry, and improve the quality of meat and eggs
3. Can change the physiology of bacteria, interfere the metabolism of bacteria and prevent the infection of germs to the organism.
4. Promoting digestion and absorption of nutrients.
5. Can delay or block the oxidation process of the feed, stabilize the feed quality, improve the feed sanitation and improve the feed palatability.
6. Is rich in chitin (pupa chitin), is beneficial to removing in vivo waste, and ensures the normal work of the physiological functions of livestock and poultry.
7. Rich in antibacterial peptide: has unique immunity function, broad-spectrum antibacterial effect, and can kill invasive bacteria.
8. The beneficial bacteria are rich: can greatly improve the feed conversion rate and reduce the culture cost.
9. Improving gastrointestinal function, and treating diarrhea of livestock and fowl.
10. Can improve the laying rate of poultry and prolong the laying period. Improve the conception rate, the farrowing rate and the farrowing survival rate of the sows.
At present, the price of high-content Tabanus pulp ferment protein powder in the international market is up to $ 45/kg.
The investment for building a factory for treating 2000 tons of kitchen waste solids in one day through anaerobic fermentation of organic fertilizer is about 3500 ten thousand yuan, if a place which is of the same scale and specially uses hermetia illucens larvae to treat the food waste solids is built, the investment is only 300 ten thousand yuan, the economic benefit difference generated by the latter is more than ten times higher than that of the former, and the market potential of the hermetia illucens for treating the waste solids is very large. In addition, the hermetia illucens has the characteristics of high growth speed, strong breeding and regeneration capabilities, high food conversion rate and the like, and has a very wide prospect if large-scale cultivation is realized.
Disclosure of Invention
The invention aims to provide a method for extracting protein powder by using food waste solids to culture hermetia illucens, aiming at the national conditions of China. Not only can effectively reduce the pollution of food waste solids to the environment, but also can extract high-added-value and high-content Tabanus pulp enzyme protein powder.
In order to achieve the purpose, the technical scheme adopted by the invention is a method for extracting protein powder by utilizing food waste solids to culture hermetia illucens, and is characterized in that the protein powder is prepared from the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 550-650 parts of anhydrous ether, 900-1000 parts of deionized water, 7-10 parts of sodium hydroxide, 0.4-0.7 part of trypsin, 0.3-0.5 part of chymotrypsin and 2-3 parts of activated carbon;
further, the method for extracting the protein powder by utilizing the catering waste solids to culture the hermetia illucens comprises the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 600 parts of anhydrous ether, 950 parts of deionized water, 8 parts of sodium hydroxide, 0.5 part of trypsin, 0.3 part of chymotrypsin and 3 parts of activated carbon;
preferably, the high-content gadfly plasma ferment protein powder extracted from black soldier flies is a commercially available product of anhydrous ether, deionized water, sodium hydroxide, trypsin, chymotrypsin and activated carbon;
the method for preparing the protein powder extracted from the hermetia illucens cultured by utilizing the food waste solids comprises the following steps:
a. pretreatment: firstly, carrying out ultraviolet sterilization and drying on the black water , and crushing the black water into fine powder of 200 meshes;
b. degreasing the mixture: adding 550-650 parts of anhydrous ether into 100 parts of powder, heating to 40 ℃, stirring at a low speed for 3 hours, and filtering oil and ether liquid to obtain degreased powder;
c. leaching: adding 900-1000 parts of deionized water into 100 parts of degreased powder, adjusting the pH value to 8 by using 7-10 parts of sodium hydroxide, and heating to 90-100 ℃ to soak for 30 minutes;
d. double-enzyme hydrolysis: cooling the liquid to 50 ℃, adding 0.4-0.7 part of trypsin and 0.3-0.5 part of chymotrypsin, and extracting for 5 hours;
e. and (3) decoloring: cooling the liquid to 25 ℃, adding 2-3 parts of activated carbon, stirring at a low speed for 2 hours, and filtering;
f. spray drying: and (3) feeding the liquid material into a spray dryer, wherein the inlet temperature is 45 ℃, the outlet temperature is 180-73 ℃, the feeding amount is 20ml/min, and the rotating speed is 10000r/min, and performing spray drying to obtain the high-content Tabanus pulp enzyme protein powder.
The invention has the following advantages:
1. by utilizing the characteristics of large population and large quantity of food wastes in China, solid matters are obtained after solid-liquid separation of the food wastes, and the solid matters are used for large-scale and low-cost cultivation of hermetia illucens; the liquid is separated into grease and water by a centrifuge, namely the grease is used for producing biodiesel (applied for another patent), and the waste water is used for producing the organic humic acid saline-alkali soil modifier (applied for another patent), so that zero landfill and zero incineration of the food waste are achieved, and the environment-friendly biological organic humic acid modifier is added with tiles for green Chinese added bricks;
2. the high-content gadfly pulp enzyme protein powder is extracted by utilizing a large amount of food and beverage waste solids to breed the hermetia illucens, so that the pollution of food and beverage waste to the environment is reduced, and the high-content gadfly pulp enzyme protein powder with high added value is extracted;
3. the preparation method is simple and reasonable, can ensure the product quality, is easy to operate and has low device investment.
Detailed Description
The present invention is described in further detail below with reference to specific examples.
Example 1: the method for extracting the protein powder by utilizing the solid matters of the food wastes to breed the hermetia illucens comprises the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 600 parts of anhydrous ether, 950 parts of deionized water, 10 parts of sodium hydroxide, 0.5 part of trypsin, 0.3 part of chymotrypsin and 3 parts of activated carbon;
the method for preparing the protein powder extracted from the hermetia illucens cultured by using the food waste solids comprises the following steps:
a. pretreatment: firstly, carrying out ultraviolet sterilization and drying on the black water , and crushing the black water into fine powder of 200 meshes;
b. degreasing the mixture: adding 600 parts of anhydrous ether into 100 parts of powder, heating to 40 ℃, stirring at a low speed for 3 hours, and filtering oil and ether liquid to obtain degreased powder;
c. leaching: adding 950 parts of deionized water into 100 parts of the degreased powder, adjusting the pH value to 9 by using 10 parts of sodium hydroxide, and heating to 95 ℃ to soak for 30 minutes;
d. double-enzyme hydrolysis: cooling the liquid to 50 ℃, adding 0.4 part of trypsin and 0.3 part of chymotrypsin, and extracting for 5 hours;
e. and (3) decoloring: cooling the liquid to 25 ℃, adding 3 parts of active carbon, stirring at a low speed for 2 hours, and filtering;
f. spray drying: and (3) feeding the liquid material into a spray dryer, wherein the inlet temperature is 45 ℃, the outlet temperature is 180-80 ℃, the feeding amount is 20ml/min, and the rotating speed is 10000r/min, and performing spray drying to obtain the high-content Tabanus pulp enzyme protein powder.
Example 2: the method for extracting the protein powder by utilizing the solid matters of the food wastes to breed the hermetia illucens comprises the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 580 parts of anhydrous ether, 920 parts of deionized water, 9 parts of sodium hydroxide, 0.4 part of trypsin, 0.4 part of chymotrypsin and 2.5 parts of activated carbon;
the method for preparing the protein powder extracted from the hermetia illucens cultured by using the food waste solids comprises the following steps:
a. pretreatment: firstly, carrying out ultraviolet sterilization and drying on the black water , and crushing the black water into fine powder of 200 meshes;
b. degreasing the mixture: adding 580 parts of anhydrous ether into 100 parts of powder, heating to 40 ℃, stirring at low speed for 3 hours, and filtering oil and ether liquid to obtain degreased powder;
c. leaching: adding 920 parts of deionized water into 100 parts of degreased powder, adjusting the pH value to 8 by using 9 parts of sodium hydroxide, and heating to 95 ℃ to soak for 30 minutes;
d. double-enzyme hydrolysis: cooling the liquid to 50 ℃, adding 0.4 part of trypsin and 0.4 part of chymotrypsin, and extracting for 5 hours;
e. and (3) decoloring: cooling the liquid to 25 ℃, adding 2.5 parts of activated carbon, stirring at a low speed for 2 hours, and filtering;
f. spray drying: and (3) feeding the liquid material into a spray dryer, wherein the inlet temperature is 45 ℃, the outlet temperature is 173-73 ℃, the feeding amount is 20ml/min, and the rotating speed is 10000r/min, and performing spray drying to obtain the high-content Tabanus pulp enzyme protein powder.
Example 3: the method for extracting the protein powder by utilizing the solid matters of the food wastes to breed the hermetia illucens comprises the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 650 parts of anhydrous ether, 900 parts of deionized water, 8 parts of sodium hydroxide, 0.5 part of trypsin, 0.4 part of chymotrypsin and 3 parts of activated carbon;
the method for preparing the protein powder extracted from the hermetia illucens cultured by using the food waste solids comprises the following steps:
a. pretreatment: firstly, carrying out ultraviolet sterilization and drying on the black water , and crushing the black water into fine powder of 200 meshes;
b. degreasing the mixture: adding 650 parts of anhydrous ether into 100 parts of powder, heating to 40 ℃, stirring at a low speed for 3 hours, and filtering oil and ether liquid to obtain degreased powder;
c. leaching: adding 900 parts of deionized water into 100 parts of degreased powder, adjusting the pH value to 7 by using 8 parts of sodium hydroxide, and heating to 95 ℃ to soak for 30 minutes;
d. double-enzyme hydrolysis: cooling the liquid to 50 ℃, adding 0.5 part of trypsin and 0.4 part of chymotrypsin, and extracting for 5 hours;
e. and (3) decoloring: cooling the liquid to 25 ℃, adding 3 parts of active carbon, stirring at a low speed for 2 hours, and filtering;
f. spray drying: and (3) feeding the liquid material into a spray dryer, wherein the inlet temperature is 45 ℃, the outlet temperature is 175-75 ℃, the feeding amount is 20ml/min, and the rotating speed is 10000r/min, and performing spray drying to obtain the high-content Tabanus pulp enzyme protein powder.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and it will be apparent to those skilled in the art that various modifications and variations can be made in the spirit and principle of the present invention and that equivalent modifications and substitutions and the like are included in the scope of the present invention.
Claims (6)
1. The method for extracting protein powder by using food waste solids to breed hermetia illucens and the preparation method thereof are characterized in that the protein powder is prepared from the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 550-650 parts of anhydrous ether, 900-1000 parts of deionized water, 7-10 parts of sodium hydroxide, 0.4-0.7 part of trypsin, 0.3-0.5 part of chymotrypsin and 2-3 parts of activated carbon.
2. The method for extracting protein powder by utilizing the food waste solids to breed the hermetia illucens as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 100 parts of hermetia illucens powder, 550-650 parts of anhydrous ether, 900-1000 parts of deionized water, 7-10 parts of sodium hydroxide, 0.4-0.7 part of trypsin, 0.3-0.5 part of chymotrypsin and 2-3 parts of activated carbon.
3. The method for extracting protein powder from hermetia illucens cultured by using food waste solids according to claim 1 or 2, wherein the process for extracting high-content Tabanus slurry enzyme protein powder comprises the following steps: firstly, carrying out ultraviolet sterilization and drying on the black water , crushing the black water into fine powder of 200 meshes, and carrying out degreasing on the fine powder: adding deionized water into the defatted powder, adjusting pH to 8 with sodium hydroxide, heating to a certain degree, soaking for a period of time, and adding 0.5% trypsin chymotrypsin for double-enzyme hydrolysis; then adding active carbon, stirring at a low speed for 2 hours, filtering, decoloring and finally carrying out spray drying to obtain the high-content Tabanus pulp enzyme protein powder.
4. The method for extracting protein powder from hermetia illucens cultivated by food waste solids according to claim 1 or 2, wherein the degreasing process of the hermetia illucens powder is as follows: and adding 550-650 parts of anhydrous ether into 100 parts of powder, heating to 40 ℃, stirring at a low speed for 3 hours, and filtering out grease and ether to obtain degreased powder.
5. The method for extracting protein powder from hermetia illucens cultured by using food waste solids according to claim 1 or 2, wherein the double-enzyme hydrolysis is carried out by the following steps: adding 0.4-0.7 part of trypsin and 0.3-0.5 part of chymotrypsin into 900-1000 parts of deionized water, and extracting for 5 hours.
6. The method for extracting protein powder from hermetia illucens cultured by using food waste solids according to claim 1 or 2, wherein the anhydrous ether, deionized water, sodium hydroxide, trypsin, chymotrypsin and activated carbon are all commercially available products.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113480353A (en) * | 2021-06-11 | 2021-10-08 | 湛江市博泰生物化工科技实业有限公司 | Process for high-value extraction of byproduct organic potassium sulfate compound fertilizer from hermetia illucens |
| CN113854403A (en) * | 2021-10-11 | 2021-12-31 | 杭州谷胜科技有限公司 | Animal feed prepared from hermetia illucens, and preparation method and application thereof |
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2019
- 2019-07-08 CN CN201910617311.5A patent/CN112195213A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113480353A (en) * | 2021-06-11 | 2021-10-08 | 湛江市博泰生物化工科技实业有限公司 | Process for high-value extraction of byproduct organic potassium sulfate compound fertilizer from hermetia illucens |
| CN113854403A (en) * | 2021-10-11 | 2021-12-31 | 杭州谷胜科技有限公司 | Animal feed prepared from hermetia illucens, and preparation method and application thereof |
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