CN104206359B - A kind of method of utilizing micro-algae and other microorganism composite bait artemia cultures - Google Patents
A kind of method of utilizing micro-algae and other microorganism composite bait artemia cultures Download PDFInfo
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- CN104206359B CN104206359B CN201410413004.2A CN201410413004A CN104206359B CN 104206359 B CN104206359 B CN 104206359B CN 201410413004 A CN201410413004 A CN 201410413004A CN 104206359 B CN104206359 B CN 104206359B
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Abstract
The invention provides a kind of method of utilizing micro-algae and other microorganism composite bait artemia cultures, comprise following step, 1), the preparation of micro-algae and other microorganism composite baits; 2), the hatching of artemia nauplii, after hatching 24h, separate and obtain Artemia nauplii; 3), inoculation and the cultivation of Artemia nauplii, wherein, in halogen worm nutrient solution, inoculation has micro-algae; 4), the cultivation of halogen worm, after Artemia nauplii inoculation 24h, start other microorganisms of throwing something and feeding of the first round, and remain the concentration of micro-algae. The present invention adopts a step food chain mode to cultivate, nutritious, cultivation is convenient, nontoxic, cost is extremely low. And micro-algae and the somatomedin that other other microorganism composite baits contain abundant species, can improve halogen worm resistance, growth is rapid, and cultivation density can reach 1000～10,000ind/L.
Description
Technical field
The present invention relates to artemia culture field, especially relate to one and utilize micro-algae and other microorganisms compoundThe method of artemia culture.
Background technology
Halogen worm (Artemiasp.) claim again fairy shrimp, is that a kind of small-sized crust of one of wide temperature salt tolerance is movingThing can be lived in fresh water and salt water. Halogen worm contains abundant nutriment, and dry product contains protein60%, the growth of fat 20% and abundant higher unsaturated fatty acid, trace element and abundant species because ofSon. Halogen worm is as a kind of living body bait of high-quality, and in aquaculture, application is very extensive. But at presentArtemia resource is limited in the world, and adult ' Luchung ' insect resource mainly relies on wild breeding, propagates in the world halogen artificiallyThe technology content of worm is also lower, and the extensive cultivation that is mainly the countries such as Vietnam is main. That commonly uses at present supportsCulturing method be at initial stage of cultivation taking rich water and the abundant bittern of supplementary biological feed as main, transparency is protectedBe held in about 30cm, along with growing of halogen worm, the organic debris of need to progressively throwing something and feeding, as bran,Oilseed meals, corn, chicken manure, cattle and sheep excrement etc. Particle is less than 50 μ m, feeding volume 30-50kg/ daysHectare, gradation to feed. Have ready conditions and can use inorganic fertilizer, weekly the per hectare water surface apply urea 20kg,Diammonium hydrogen phosphate 25kg. Therefore, the halogen worm high-density breeding technology of high technology content is urgently to be resolved hurrily.
Summary of the invention
The problem to be solved in the present invention is that one is utilized micro-algae and other microorganism composite bait artemia culturesMethod, realize a step food chain high-density breeding halogen worm.
For solving the problems of the technologies described above, the technical solution used in the present invention is: one is utilized micro-algae and otherThe method of microorganism composite bait artemia culture, comprises following step,
1), the preparation of micro-algae and other microorganism composite baits;
2), the hatching of artemia nauplii, after hatching 24h, separate and obtain Artemia nauplii;
3), inoculation and the cultivation of Artemia nauplii, wherein, in halogen worm nutrient solution, inoculation has micro-Algae;
4), the cultivation of halogen worm, after Artemia nauplii inoculation 24h, start throwing something and feeding of the first roundOther microorganisms, and remain the concentration of micro-algae.
Preferably, step 1) in, described micro-algae is salt algae, chlorella, split kettle algae, spirulina, goldOne in algae, haematococcus pluvialis or two or more; Described other microorganisms are yeast or photosynthetic bacteriaOr both mixtures.
Preferably, step 2) in, the condition of artemia nauplii hatching is that artemia hatching solution salinity is 10～30‰, pH is 7～9, and intensity of illumination is 1500～3000lux, continues after inflation hatching 24h, and separation obtainsGet Artemia nauplii.
Preferably, step 3) in, inoculation and the cultivation of Artemia nauplii comprise the steps:
(a), preparation halogen worm nutrient solution, halogen worm nutrient solution salinity is 30～150 ‰, pH is 7～8,Temperature is 25～30 DEG C, and DO is 4～10ppm, and ammonia-nitrogen content is lower than 5ppm, fully aeration 24h standbyWith;
(b), inoculation fresh cultured salt algae, make the transparency of water body at 15～25cm.
(c), Artemia nauplii is with the density inoculation of 3～10ind/ml.
Preferably, in described step (4), the concentration basic controlling of other microorganisms in halogen worm nutrient solution0.5～1.5 × 108Ind/ml, and supplementary salt algae is to maintain the transparency of 15～25cm.
The measuring method of the artemia nauplii density that the present invention adopts is to get 100 the artemia hatching solution from mixingμ l halogen worm 5 times, counting respectively under anatomical lens, calculates five mean value as artemia naupliiDensity; The long measuring method of halogen polypide adopting is random 10 of the halogen worms of selecting, and under microscope mirror, dividesDo not measure body long, the mean value that calculates 10 data is long as the average body of halogen worm.
Advantage and good effect that the present invention has are: the present invention adopts micro-algae and other other microorganisms againClose the unique bait of bait as halogen worm, the step food chain mode of employing cultivates, nutritious, cultivation sideJust, nontoxic, cost is extremely low. And micro-algae and other other microorganism composite baits to contain kind richRich somatomedin, can improve halogen worm resistance, and growth rapidly, can be long for becoming in 15d left and rightWorm, body is long > 1cm, and also cultivation density can reach 1000～10,000ind/L. This method is turned outHalogen worm balanced in nutrition abundant, contain rich in protein and aliphatic acid, resistance is high.
Detailed description of the invention
In order to understand the present invention, below by instantiation, the present invention is further described, but does not limitDetermine protection scope of the present invention.
Embodiment mono-
1), the preparation of micro-algae and other microorganism composite baits;
The artificial salt algae of cultivating, salinity is 80 ‰, becomes to make a critical appraisal of liquid concentration higher than 5 × 107Ind/ml. CompoundOther microorganisms in bait are salt tolerant yeast.
2), the hatching of artemia nauplii;
Artemia hatching solution salinity is 30 ‰, uses the preparation of artificial sea crystal mediation running water, for subsequent use after aeration 24h.
Hatching cylinder tool 3000lux intensity of illumination, is used 28 DEG C of heating rod temperature controls, uses sodium acid carbonate to regulatePH is 8.5.
The small sea strain halogen worm one-tenth that halogen worm's ovum provides for Tianjin Haiyou Jiayin Biology Co., Ltd.Product ovum, incubation rate is more than 85%.
After hatching 24h, standing separation, obtains Artemia nauplii.
3), the inoculation of Artemia nauplii and cultivation;
Halogen worm nutrient solution uses sea crystal and running water preparation, and salinity is 80 ‰, fully aeration 24h standbyWith.
The salt algae of inoculation fresh cultured, makes the transparency of water body in 20cm left and right
Inoculation is just incubated artemia nauplii, and density is 5ind/ml.
4), the cultivation of halogen worm;
After Artemia nauplii inoculation 24h, start throwing something and feeding of the first round, yeast in halogen worm nutrient solutionConcentration basic controlling 1 × 108Ind/ml. Throw something and feed every day 3 times, and suitably supplement salt algae to maintainThe transparency of 20cm.
In breeding process, controlling water temperature is 28～30 DEG C, and pH uses sodium bicarbonate solution to regulate 5 to controlBetween 7～8, more than DO remains on 3ppm.
(5), while cultivating 20d, artemia density maintains 3ind/ml left and right. Use 120 mesh filter screens to receiveCollection halogen worm polypide, drains after use clean sea water rinses, and weight in wet base can reach 18.2kg/m3.
Embodiment bis-
1), the preparation of micro-algae and other microorganism composite baits;
Described micro-algae is the artificial haematococcus pluvialis of cultivating, and salinity is 100 ‰, become to make a critical appraisal of liquid concentration higher than5×107Ind/ml. Other microorganisms in composite bait are bacillus subtilis.
2), the hatching of artemia nauplii;
Artemia hatching solution salinity is 30 ‰, uses the preparation of artificial sea crystal mediation running water, for subsequent use after aeration 24h.
Hatching cylinder tool 3000lux intensity of illumination, is used 28 DEG C of heating rod temperature controls, uses sodium acid carbonate to regulatePH is 8.5.
The Tibet strain halogen worm one-tenth that halogen worm's ovum provides for Tianjin Haiyou Jiayin Biology Co., Ltd.Product ovum, incubation rate is more than 85%.
After hatching 24h, standing separation, obtains Artemia nauplii;
3), the inoculation of Artemia nauplii and cultivation;
Halogen worm nutrient solution uses sea crystal and running water preparation, and salinity is 100 ‰, fully after aeration 24hFor subsequent use.
The haematococcus pluvialis of inoculation fresh cultured, makes the transparency of water body in 20cm left and right
Inoculation is just incubated artemia nauplii, and density is 5ind/ml.
4), the cultivation of halogen worm;
After Artemia nauplii inoculation 24h, start throwing something and feeding of the first round, withered grass in halogen worm nutrient solutionThe concentration basic controlling of bacillus is 1 × 108Ind/ml. Throw something and feed every day 3 times, and it is raw suitably to supplement rainHaematococcus is to maintain the transparency of 20cm.
In breeding process, controlling water temperature is 28～30 DEG C, and pH uses sodium bicarbonate solution to regulate 5 to controlBetween 7～8, more than DO remains on 3ppm.
(5), while cultivating 18d, artemia density maintains 8ind/ml left and right. Use 120 mesh filter screens to collectHalogen worm polypide, drains after use clean sea water rinses, and weight in wet base can reach 26kg/m3。
Embodiment tri-
1), the preparation of micro-algae and other microorganism composite baits;
Described micro-algae is the artificial chrysophyceae of cultivating, and salinity is 150 ‰, the liquid concentration that becomes to make a critical appraisal of higher than 5 ×107Ind/ml. Other microorganisms in composite bait are photosynthetic bacteria.
2), the hatching of artemia nauplii;
Artemia hatching solution salinity is 30 ‰, uses the preparation of artificial sea crystal mediation running water, for subsequent use after aeration 24h.
Hatching cylinder tool 3000lux intensity of illumination, is used 28 DEG C of heating rod temperature controls, uses sodium acid carbonate to regulatePH is 8.5.
The Tibet strain halogen worm one-tenth that halogen worm's ovum provides for Tianjin Haiyou Jiayin Biology Co., Ltd.Product ovum, incubation rate is more than 85%.
After hatching 24h, standing separation, obtains Artemia nauplii. ;
3), the inoculation of Artemia nauplii and cultivation;
Halogen worm nutrient solution uses sea crystal and running water preparation, and salinity is 150 ‰, fully after aeration 24hFor subsequent use.
The chrysophyceae of inoculation fresh cultured, makes the transparency of water body in 20cm left and right
Inoculation is just incubated artemia nauplii, and density is 5ind/ml.
4), the cultivation of halogen worm;
After Artemia nauplii inoculation 24h, start throwing something and feeding of the first round, photosynthetic in halogen worm nutrient solutionThe concentration basic controlling of bacterium is 1 × 108Ind/ml left and right. Throw something and feed every day 3 times, and suitably supplement salt algaeTo maintain the transparency of 20cm left and right.
In breeding process, controlling water temperature is 28～30 DEG C, and pH uses sodium bicarbonate solution to regulate 5 to controlBetween 7～8, more than DO remains on 3ppm.
(5), while cultivating 16d, artemia density maintains 7ind/ml left and right. Use 120 mesh filter screens to receiveCollection halogen worm polypide, drains after use clean sea water rinses, and weight in wet base can reach 24.5kg/m3。
Above embodiments of the invention are had been described in detail, but described content is only the inventionPreferred embodiment, can not be considered to for limiting practical range of the present invention. All according to the invention scopeEqualization variation and the improvement etc. done, within all should still belonging to this patent covering scope.
Claims (4)
1. a method of utilizing micro-algae and other microorganism composite bait artemia cultures, is characterized in that: comprise following step,
1), the preparation of micro-algae and other microorganism composite baits;
2), the hatching of artemia nauplii, after hatching 24h, separate and obtain Artemia nauplii;
3), inoculation and the cultivation of Artemia nauplii, wherein, in halogen worm nutrient solution, inoculation has micro-algae;
4), the cultivation of halogen worm, after Artemia nauplii inoculation 24h, start other microorganisms of throwing something and feeding of the first round, and remain the concentration of micro-algae;
Wherein, step 3) in, inoculation and the cultivation of Artemia nauplii comprise the steps:
(a), preparation halogen worm nutrient solution, halogen worm nutrient solution salinity is 30～150 ‰, pH is 7～8, temperature is 25～30 DEG C, DO is 4～10ppm, ammonia-nitrogen content is lower than 5ppm, fully for subsequent use after aeration 24h;
(b), inoculation fresh cultured salt algae, make the transparency of water body at 15～25cm;
(c), Artemia nauplii is with the density inoculation of 3～10ind/ml;
Described micro-algae is salt algae, chlorella, split a kind of or two or more in kettle algae, spirulina, chrysophyceae, haematococcus pluvialis; Described other microorganisms are yeast or bacterium or both mixtures.
2. the method for utilizing micro-algae and other microorganism composite bait artemia cultures according to claim 1, is characterized in that: described yeast is salt tolerant yeast; Described bacterium is bacillus subtilis or photosynthetic bacteria.
3. the method for utilizing micro-algae and other microorganism composite bait artemia cultures according to claim 1, it is characterized in that: step 2) in, the condition of artemia nauplii hatching is, artemia hatching solution salinity is 10～30 ‰, pH is 7～9, intensity of illumination is 1500～3000lux, continues, after inflation hatching 24h, to separate and obtain Artemia nauplii.
4. the method for utilizing micro-algae and other microorganism composite bait artemia cultures according to claim 1, is characterized in that: described step 3) in, in halogen worm nutrient solution, the concentration of other microorganisms is controlled at 0.5～1.5 × 108Ind/ml, and supplementary salt algae is to maintain the transparency of 15～25cm.
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| CN105766810B (en) * | 2016-04-06 | 2019-02-22 | 鲁东大学 | A low-cost, high-density and sustainable indoor farming method for Artemia |
| CN106165790A (en) * | 2016-08-31 | 2016-11-30 | 天津海友佳音生物科技股份有限公司 | A kind of bait for artemia culture and artemia culture method |
| CN107494343A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial fecundation method of loach |
| CN109744404A (en) * | 2017-11-06 | 2019-05-14 | 南风化工集团股份有限公司 | Agreeable to the taste feed of a kind of ecology artemia and preparation method thereof |
| CN110856526A (en) * | 2018-08-22 | 2020-03-03 | 南风化工集团股份有限公司 | Brine shrimp feed for salt pan and preparation method thereof |
| WO2020190222A1 (en) * | 2019-03-19 | 2020-09-24 | Artkom Yem Sanayi̇ İç Ve Diş Ti̇caret Li̇mi̇ted Şi̇rketi̇ | A method which enables hatching of artemia eggs |
| CN110214730B (en) * | 2019-06-28 | 2021-04-27 | 中国科学院青岛生物能源与过程研究所 | Domestication method for rapidly proliferating artemia under high-temperature and high-salt environment |
| CN110754405B (en) * | 2019-11-29 | 2021-11-23 | 广东海洋大学 | Large-scale production method of artemia salina imago |
| CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | A method for preparing high-value aquatic bait by utilizing microalgae and Artemia |
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| CN1066559A (en) * | 1992-05-30 | 1992-12-02 | 天津长芦汉沽盐场 | The salt pan halogen insect propagation method that adapts to the salt industry production environment |
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| AU2003299578A1 (en) * | 2002-11-26 | 2004-06-18 | Gordon H. Sato | Microbial feedstock for filter feeding aquatic organisms |
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Patent Citations (3)
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|---|---|---|---|---|
| CN1066559A (en) * | 1992-05-30 | 1992-12-02 | 天津长芦汉沽盐场 | The salt pan halogen insect propagation method that adapts to the salt industry production environment |
| CN102342262A (en) * | 2010-08-02 | 2012-02-08 | 佛山市顺德区汉楚电子塑料制品有限公司 | Artemia ecologic toy and production method thereof |
| CN203646336U (en) * | 2013-09-26 | 2014-06-18 | 天津海友佳音生物科技股份有限公司 | Artemia culture device |
Non-Patent Citations (1)
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| 不同饵料对卤虫生长、总脂含量及脂肪酸组成的影响;彭瑞冰等;《生物学杂志》;20131018;第30卷(第5期);第68-72页 * |
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