CN105707473B - A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation - Google Patents
A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation Download PDFInfo
- Publication number
- CN105707473B CN105707473B CN201610129696.7A CN201610129696A CN105707473B CN 105707473 B CN105707473 B CN 105707473B CN 201610129696 A CN201610129696 A CN 201610129696A CN 105707473 B CN105707473 B CN 105707473B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- herbal medicine
- chinese herbal
- bacillus licheniformis
- green
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 91
- 230000004151 fermentation Effects 0.000 title claims abstract description 91
- 241000411851 herbal medicine Species 0.000 title claims abstract description 45
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 30
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 30
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 25
- 230000002155 anti-virotic Effects 0.000 title claims abstract description 14
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 43
- 239000002351 wastewater Substances 0.000 claims abstract description 18
- 239000000203 mixture Substances 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 9
- 238000004806 packaging method and process Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001704 evaporation Methods 0.000 claims abstract description 4
- 239000002609 media Substances 0.000 claims description 21
- 235000021190 leftovers Nutrition 0.000 claims description 17
- 230000001954 sterilising Effects 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 11
- 241000238557 Decapoda Species 0.000 claims description 8
- 230000000844 anti-bacterial Effects 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L Dipotassium phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L cacl2 Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 7
- 239000001110 calcium chloride Substances 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 235000019733 Fish meal Nutrition 0.000 claims description 4
- 241000276707 Tilapia Species 0.000 claims description 4
- 238000007792 addition Methods 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 4
- 239000004467 fishmeal Substances 0.000 claims description 4
- 241000239366 Euphausiacea Species 0.000 claims description 3
- 239000009636 Huang Qi Substances 0.000 claims description 3
- 238000010411 cooking Methods 0.000 claims description 3
- 239000001963 growth media Substances 0.000 claims description 3
- 241000605422 Asparagus asparagoides Species 0.000 claims description 2
- 241000238366 Cephalopoda Species 0.000 claims description 2
- 229920002101 Chitin Polymers 0.000 claims description 2
- DJHJJVWPFGHIPH-OODMECLYSA-N Chitin Chemical compound O[C@@H]1C(NC(=O)C)[C@H](O)OC(CO)[C@H]1COC[C@H]1C(NC(C)=O)[C@@H](O)[C@H](COC[C@H]2C([C@@H](O)[C@H](O)C(CO)O2)NC(C)=O)C(CO)O1 DJHJJVWPFGHIPH-OODMECLYSA-N 0.000 claims description 2
- 244000247747 Coptis groenlandica Species 0.000 claims description 2
- 235000002991 Coptis groenlandica Nutrition 0.000 claims description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 claims description 2
- 241000276435 Gadus Species 0.000 claims description 2
- 241000273924 Harengula Species 0.000 claims description 2
- 235000013717 Houttuynia Nutrition 0.000 claims description 2
- 240000000691 Houttuynia cordata Species 0.000 claims description 2
- 235000013719 Houttuynia cordata Nutrition 0.000 claims description 2
- 241000252234 Hypophthalmichthys nobilis Species 0.000 claims description 2
- 241001275898 Mylopharyngodon piceus Species 0.000 claims description 2
- 241000237509 Patinopecten sp. Species 0.000 claims description 2
- 241001499733 Plantago asiatica Species 0.000 claims description 2
- 240000004064 Poterium sanguisorba Species 0.000 claims description 2
- 235000008291 Poterium sanguisorba Nutrition 0.000 claims description 2
- 235000008282 Sanguisorba officinalis Nutrition 0.000 claims description 2
- 240000006079 Schisandra chinensis Species 0.000 claims description 2
- 235000008422 Schisandra chinensis Nutrition 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 235000020637 scallop Nutrition 0.000 claims description 2
- 241000205585 Aquilegia canadensis Species 0.000 claims 1
- 241001143928 Psilurus incurvus Species 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 abstract description 23
- 108090000790 Enzymes Proteins 0.000 abstract description 23
- 108091005771 Peptidases Proteins 0.000 abstract description 11
- 235000019833 protease Nutrition 0.000 abstract description 11
- 229920002472 Starch Polymers 0.000 abstract description 9
- 235000019698 starch Nutrition 0.000 abstract description 9
- 239000008107 starch Substances 0.000 abstract description 9
- 150000004676 glycans Polymers 0.000 abstract description 8
- 239000004615 ingredient Substances 0.000 abstract description 8
- 229920001282 polysaccharide Polymers 0.000 abstract description 8
- 239000005017 polysaccharide Substances 0.000 abstract description 8
- 150000004804 polysaccharides Polymers 0.000 abstract description 8
- 239000003674 animal food additive Substances 0.000 abstract description 7
- 230000003115 biocidal Effects 0.000 abstract description 5
- 102000035443 Peptidases Human genes 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract description 4
- 239000006041 probiotic Substances 0.000 abstract description 4
- 230000000529 probiotic Effects 0.000 abstract description 4
- 235000018291 probiotics Nutrition 0.000 abstract description 4
- 238000010564 aerobic fermentation Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 230000000192 social effect Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 description 23
- 229940088598 Enzyme Drugs 0.000 description 21
- 230000000694 effects Effects 0.000 description 16
- 230000035611 feeding Effects 0.000 description 16
- 235000018102 proteins Nutrition 0.000 description 16
- 238000004519 manufacturing process Methods 0.000 description 11
- 102000033147 ERVK-25 Human genes 0.000 description 7
- 239000004365 Protease Substances 0.000 description 7
- 230000002255 enzymatic Effects 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 244000052616 bacterial pathogens Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000005469 granulation Methods 0.000 description 4
- 230000003179 granulation Effects 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 240000001519 Verbena officinalis Species 0.000 description 3
- 235000018718 Verbena officinalis Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 230000001737 promoting Effects 0.000 description 3
- 240000006439 Aspergillus oryzae Species 0.000 description 2
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 2
- 210000002421 Cell Wall Anatomy 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000024881 catalytic activity Effects 0.000 description 2
- 150000001805 chlorine compounds Chemical class 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000001079 digestive Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 230000002708 enhancing Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 231100000224 toxic side effect Toxicity 0.000 description 2
- 108010011619 6-Phytase Proteins 0.000 description 1
- MQZIGYBFDRPAKN-QISQUURKSA-N 6-hydroxy-3-[(1E,3E,5E,7E,9E,11E,13E,15E,17E)-18-(4-hydroxy-2,6,6-trimethyl-3-oxocyclohexen-1-yl)-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenyl]-2,4,4-trimethylcyclohex-2-en-1-one Chemical compound CC=1C(=O)C(O)CC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)C(=O)C(O)CC1(C)C MQZIGYBFDRPAKN-QISQUURKSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000202807 Glycyrrhiza Species 0.000 description 1
- 210000000474 Heel Anatomy 0.000 description 1
- 241000588767 Proteus vulgaris Species 0.000 description 1
- 229940007042 Proteus vulgaris Drugs 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229940076185 Staphylococcus aureus Drugs 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive Effects 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000000433 anti-nutritional Effects 0.000 description 1
- 102000004965 antibodies Human genes 0.000 description 1
- 108090001123 antibodies Proteins 0.000 description 1
- 239000001168 astaxanthin Substances 0.000 description 1
- 229940022405 astaxanthin Drugs 0.000 description 1
- 235000013793 astaxanthin Nutrition 0.000 description 1
- 230000001580 bacterial Effects 0.000 description 1
- 230000003385 bacteriostatic Effects 0.000 description 1
- 230000001488 breeding Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 230000004059 degradation Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001627 detrimental Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079919 digestives Enzyme preparations Drugs 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000008361 herbal raw material Substances 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 230000000813 microbial Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Polymers 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000011819 refractory material Substances 0.000 description 1
- 230000000717 retained Effects 0.000 description 1
- 230000001624 sedative Effects 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Abstract
The invention discloses a kind of methods for preparing the feeding antivirus somatotropic agent of green using aquatic product protein and Chinese herbal medicine hot fermentation, including following key step: spare after fresh low-value aquatic product protein is rubbed or aquatic products processing wastewater is concentrated by evaporation to protein content is 15%~20% spare；It is spare that Chinese herbal medicine is smashed it through into 60 meshes；Aquatic products processing wastewater after the low-value aquatic product protein of rubbing or concentration is mixed with smashed Chinese herbal medicine, and adds nutrients and water mixing as fermentation medium into mixture；Accessed on fermentation medium bacillus licheniformis resistant to high temperature (Bacillus licheniformis) HL-3 progress solid-state aerobic fermentation；Product after fermentation is prepared into feed additives products after drying, crushing and packaging.The method of the present invention can prepare a kind of feeding antivirus somatotropic agent of green rich in small peptide, Chinese herbal medicine effective ingredients, thermostable proteinase, high temperature resistant non-starch polysaccharide enzyme and probiotics these types ingredient, it has good growth promotion and disease-resistant function, feeding antibiotic can be substituted completely in feedstuff industry, there is good application prospect and social effect.
Description
Technical field
The feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation the present invention relates to a kind of
Method belongs to the technical field of feed addictive preparation.
Background technique
Since the 1950s, antibiotic is widely used in animal feeding and feed, chemical synthetic drug, has swashed
The growth promotions health-care agent such as element, beta-stimulants, heavy metal, sedative.Application of the growth promotions health-care agent such as antibiotic in feed is gone through
History is long-standing, it creates the revolution of aquaculture and feed industry so that breeding scale has obtained fast in worldwide
Speed development, the production quantity and feed conversion rate of animal are greatly improved.But the achievement of industrial civilization is all as in all
As having its negative effect, for a long time using non-green feed addictives such as antibiotic, following problem is resulted in: poultry
Fowl and aquatic products product drug residue are serious, are directly detrimental to health；It is competing to lack market for livestock and poultry and aquatic products deterioration in quality
Strive power and trust；Bacterial drug resistance enhancing, antibody-resistant bacterium increase, and threaten human security；Destroy ecological environment etc..With feed
The fast development of industry and animal husbandry and fishery industry, the livestock products and aquatic products in China are changed into interim surplus from shortage at present,
People's whether safe, health more concerned with food, thus green consumption has become development trend from now on, W has been added in China in addition
TO, developed country require to be increasingly stringenter to the agricultural product of China's export, and medicament residue problem becomes China's livestock products at present
One of the major obstacle gone abroad with aquatic products.Therefore exploitation, promotion and application novel green feed addictive gesture is accelerated to exist
It must go.
Green feed additive, most basic requirement be have no toxic side effect, noresidue, and also want that growth of animal can be promoted.
Current internationally recognized growth promotion green feed additive is mainly include the following types: small peptide, enzyme preparation, plant product-derived and its mention
Take object, probiotics, oligosaccharides and yeast class product etc..
Enzyme preparation has noresidue, pollution-free as a kind of feed addictive, improves the utility value of feed, improves animal
The effects of production performance and product quality.Fodder enzyme preparation can substantially be divided into two class of digestive ferment and non-digestive ferment, usually seek
Feeding level plays a role.For example livestock and poultry alimentary tract endogenous enzyme activity is improved, supplement the deficiency of endogenous enzymes；Plant cell wall is destroyed,
Improve the utilization efficiency of feed；The anti-nutritional factors in feed is eliminated, the digestion and absorption of nutriment is promoted.Currently, enzyme preparation
Feed industry is had been widely used for, mainly there are the enzyme preparations such as protease, non-starch polysaccharide enzyme and phytase.However, in the market
The existing more non-refractories of fodder enzyme preparation, loss of activity is very big in feed high temperature granulation process.
In recent years, small peptide is concerned as a kind of green feed additive for promoting growth in feed industry.Small peptide at present
Preparation method has enzymatic isolation method and microbe fermentation method.It is using protein raw materials as substrate, with protease that enzymatic isolation method, which produces active peptide,
Enzymolysis, produce small-molecular peptides.Enzymatic isolation method is easily controllable, and action condition is mildly suitble to the production of active peptide.But enzymatic isolation method
Need a large amount of protease, high production cost.Therefore, enzymatic isolation method prepares active peptide and is chiefly used in food and pharmaceuticals industry, low side
Feeding activity peptide product is seldom using the method for enzymatic hydrolysis.Microbe fermentation method is using macro-molecular protein as raw material, and access is laid eggs
The microorganism of white enzyme generates small-molecular peptides using the enzymolysis for the protease that fermentation process generates.Fermentation method is egg
The fermenting and producing and enzymolysis process of white enzyme organically combine, and reduce the manufacturing cost of active peptide, are more suitable for active peptide
Production.Patent CNZL200410029787.0 " a kind of activated peptide forage additive and preparation method and purposes " discloses a kind of hair
The method that ferment hair prepares small peptide feed additive, it is one or more of mixtures with vegetable protein and animal protein, warp
The fermentative degradations such as bacillus make the control of its degree of hydrolysis 20~35%, and small peptide feed addition then can be obtained after post treatment
Agent.Small peptide has good application prospect in feed industry as a kind of novel green feed additive for promoting growth, however at present
There is no be widely used small peptide in feed industry.Either the small peptide of enzymatic isolation method production or fermentation method production is small
Peptide, high production cost constrain small peptide in the extensive use of feed industry.In addition, the small peptide product of prior art preparation its
Growth-promoting long component only has a kind of ingredient of small peptide, excessively single, and growth promotion performance is not prominent enough.
Chinese herbal medicine is the rarity in Chinese medicine treasure-house, and Chinese herbal medicine has been opened as feed addictive in Ancient Times in China
Begin.Chinese herbal medicine, which has, to have no toxic side effect, is not easy to cause the characteristics such as resistance, noresidue, as a kind of feeding antibiotic
Green substitute has been widely recognized in feed industry.From the 1980s, Chinese herbal medicine has been used for making feeding anti-
Raw element substitute, and achieve certain application effect.After Chinese herbal medicine undergoes microbial fermentation, effective component is sufficiently released
It puts, so that it is had more bioactivity and curative effect is even more research hotspot in recent years.Chinese feed (2010,419(15): 13-17) document
It discloses isolated aspergillus oryzae from different herbal raw materials to ferment to Chinese herbal medicine, fermentation material cooking liquor is to large intestine bar
Bacterium, staphylococcus aureus, proteus vulgaris and salmonella typhimurium bacteriostatic activity respectively relatively control improve 36.03%,
60.83%,27.64%,41.32%.Currently, the strain that Chinese herbal medicine fermentation mainly uses has the bacterium such as bacillus licheniformis and aspergillus oryzae
Kind.Mainly the cell wall main component of Chinese herbal medicine is degraded using the non-starch polysaccharide enzyme that these microorganisms generate, so that
Chinese herbal medicine effective ingredients release.However, existing Chinese herbal medicine fermenting microbe is all the strain of room temperature, during the fermentation
The activity of the non-starch polysaccharide enzyme of generation can lose totally in the high temperature granulation process of product drying and feed.Thus, it is existing
The preparation of Chinese herbal medicine fermentation technique fermentation of Chinese herbal medicine the effect of can only playing Chinese herbal medicine in animal body, can not play
The effect of enzyme preparation.
Summary of the invention
The problem of for technologies of preparing such as current enzyme preparation, small peptide and fermentation of Chinese herbal medicine, the present invention provides one
The method that kind prepares the feeding antivirus somatotropic agent of green using aquatic product protein and Chinese herbal medicine hot fermentation.The method of the present invention can be prepared
It obtains a kind of rich in small peptide, Chinese herbal medicine effective ingredients, thermostable proteinase, high temperature resistant non-starch polysaccharide enzyme and probiotics these types
The feeding antivirus somatotropic agent of green of ingredient.
To solve the above-mentioned problems, the technical solution adopted in the present invention is as follows: a kind of to utilize aquatic product protein and Chinese herbal medicine
The method of the feeding antivirus somatotropic agent of hot fermentation preparation green, includes the following steps:
(1) by the high temperature resistant bacillus licheniformis of slant preservation (Bacillus licheniformis) HL-3 is linked into and goes out
It is cooling after bacterium
LB liquid medium in, in 55 DEG C, 150r/min cultivate 12h, be prepared into bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor is spare；
(2) spare or aquatic products processing wastewater is concentrated by evaporation to protein contains after rubbing fresh low-value aquatic product protein
Amount is spare for 15%~20%；
(3) that Chinese herbal medicine is smashed it through 60 meshes is spare；
(4) during the aquatic products processing wastewater and step (3) of the low-value aquatic product protein or concentration that rub step (2) crush
Herbal medicine is the ratio mixing of 0.5 ~ 1.5:1 in mass ratio；
(5) nutrients and water are added into the mixture that step (4) obtains, and is mixed, and final moisture content control is obtained
Fermentation medium in 40%~80% range；
(6) accessed into the fermented and cultured that step (5) obtains bacillus licheniformis prepared by step (1) (Bacillus licheniformis) HL-3 seed liquor, and mix；
(7) fermentation of raw material: the fermentation medium for the addition bacterium that step (6) obtain is fermented in following manner:
Fermentation medium is divided in the fermentation tray sterilized, and charging is with a thickness of 4cm~8cm, and fermented and cultured primary surface covers in tray
The wet gauze of sterilizing is covered, then tray is placed in the shelf top fermentation in sterilized solid fermentation room, controls environment temperature 55
DEG C ~ 60 DEG C, fermentation time is 48h~60h；
(8) product handling system: fermentation ends have obtained one by the product obtained after fermentation after drying, crushing and packaging
Kind compound green feed additive for promoting growth finished product of the present invention.
High temperature resistant bacillus licheniformis used in the step (1) (Bacillus licheniformis) HL-3 in
It is preserved in Guangdong Province's Culture Collection on January 18th, 2016, deposit number is GDMCC No:60003, preservation address
For 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100 Guangdong Microbes Inst.
Low-value aquatic product protein is under leftovers of tilapia, krill, shrimp head, scallop body, gadus in the step (2)
In heel, squid leftover bits and pieces, Harengula zunasi leftover bits and pieces, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces and stripped tuna leftover bits and pieces
It is a kind of.Aquatic products processing wastewater in the step (2) is the alkali-wash waste water, right of fish meal processing waste water, chitin process
One of shrimp waste water from cooking and minced fillet processing waste water.
Chinese herbal medicine in the step (3) is Radix Glycyrrhizae, Fructus Forsythiae, Asiatic plantain, perillaseed, Schisandra chinensis, Verbena officinalis, Radix Astragali, gold and silver
One of flower, granatum, garden burnet, the coptis, cordate houttuynia, kuh-seng and smilax or in which two or more mixtures.
Nutrients in the step (5) is mixing composed by wheat bran, calcium chloride, frerrous chloride and dipotassium hydrogen phosphate
Object.The additional amount of nutrients in the step (5) accounts for fermentation medium gross mass percentage with the quality of the nutrients added
Meter, is respectively as follows: wheat bran 0.01%~15%, calcium chloride 0.01%~5%, frerrous chloride 0.01%~5%, and dipotassium hydrogen phosphate 0.01%~
5%。
Bacillus licheniformis in the step (6) (Bacillus licheniformis) HL-3 inoculum concentration with access kind
The quality of sub- liquid accounts for fermentation medium gross mass percentages, is 1%~9%.
The present invention compared with prior art, has the following advantages.
(1) fermented product can raise the effect of playing enzyme preparation in animal body.Existing Chinese herbal medicine zymophyte and small
What the fermentation preparation of peptide used is all the strain of room temperature, the work of the non-starch polysaccharide enzyme and protease that generate during the fermentation
Property can be lost totally in the drying process of product preparation and high temperature granulation process for feed.Thus, prior art preparation
Fermentation of Chinese herbal medicine and small peptide product for feed can not play enzyme preparation in animal body the effect of.And this technology is using resistance to
High temperature bacillus licheniformis (Bacillus licheniformis) ferment, fermentation process generates protease resistant to high temperature
And non-starch polysaccharide enzyme, the activity of these protease and non-starch polysaccharide enzyme resistant to high temperature is in the drying of product preparation and for raising
It is not lost substantially in the high temperature granulation process of material, thus the feed addictive of this technology preparation can play enzyme system in animal body
The effect of agent.
(2) ferment effect is good.With existing Chinese herbal medicine fermentation and fermentation method preparation small peptide compared with, the present invention using
Hot fermentation, enzyme activity under conditions of high temperature significantly increase, this can also greatly improve Chinese herbal medicine fermentation and fermentation method prepare it is small
The ferment effect of peptide；In addition, raw material does not sterilize in the technology of the present invention, aquatic product protein endogenous proteinase can be given full play to
Activity, this can further increase the content of small peptide.
(3) at low cost.Compared with existing fermentation method prepares small peptide, in technical solution provided by the invention, raw material does not have to
Sterilizing, can reduce energy consumption and cost.In addition, the fermenting and producing of small peptide and the fermenting and producing of fodder enzyme preparation conjunction two are by the present invention
One, the production of fodder enzyme preparation can share part producing cost, to further decrease the production cost of product.
(4) it can effectively protect the function factor of thermal sensitivity in aquatic product protein.In technical solution of the present invention, since raw material can
With not sterilized, thus, the activity of the function factor of thermal sensitivity is effectively retained in aquatic product protein.For example, shrimp head and the South Pole
Astaxanthin in krill.
The feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation the present invention provides a kind of
Method.The method of the present invention can prepare a kind of rich in small peptide, Chinese herbal medicine effective ingredients, thermostable proteinase, high temperature resistant non-starch
The feeding antivirus somatotropic agent of polysaccharase and probiotics these types ingredient.The feeding antivirus somatotropic agent of green that the present invention obtains exists
The pig of South China, chicken, prawn culture experiment show that it can promote raising growth of animal and enhances the premunition of animal, it is bright
It is aobvious to reduce aquaculture cost, obtain significant economic benefit.
Specific embodiment
For a better understanding of the present invention, below with regard to the type of aquatic product protein, the type of Chinese herbal medicine, fermentation parameter and fermentation
The invention will be further described in conjunction with the embodiments for the selection of mode, and but the scope of the present invention is not limited thereto.
Embodiment 1: by the high temperature resistant bacillus licheniformis of slant preservation (Bacillus licheniformis) HL-3 connects
After entering to sterilizing in cooling LB liquid medium, 12h is cultivated in 55 DEG C, 150r/min, is prepared into bacillus licheniformis
(Bacillus licheniformis) HL-3 seed liquor is spare.It is spare after fresh leftovers of tilapia is rubbed, by Radix Glycyrrhizae
It is spare to smash it through 60 meshes；The Radix Glycyrrhizae of the leftovers of tilapia that 100 kilograms are rubbed and 100 kilograms of crushing mixes, and is added
4900 grams of wheat brans, 455 grams of calcium chloride, 356 grams of frerrous chlorides, 555 grams of dipotassium hydrogen phosphates and 12 kg of water mix together, are mixed
Fermentation medium after even；Be added into fermentation medium 9 kilograms of bacillus licheniformis bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor, it stirs and is uniformly mixed；Fermentation medium after inoculation is divided in the alcohol with 75%
In the fermentation tray of surface sterilization, charging is in tray with a thickness of 4 centimetres, the wet yarn of surface of material covering sterilization treatment in tray
Cloth, then tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, control environment temperature is 55 DEG C, and fermentation time is
48h；After fermentation, by the product obtained after fermentation after drying, crushing and packaging, a kind of feeding antibacterial of green has been obtained
Growth accelerator finished product.
Embodiment 2: by the high temperature resistant bacillus licheniformis of slant preservation (Bacillus licheniformis) HL-3 connects
After entering to sterilizing in cooling LB liquid medium, 12h is cultivated in 55 DEG C, 150r/min, is prepared into bacillus licheniformis
(Bacillus licheniformis) HL-3 seed liquor is spare.It is spare after fresh shrimp head is rubbed, after Verbena officinalis is crushed
It is spare to cross 60 meshes；The Verbena officinalis of the shrimp head of 110 kilograms of rubbings and 100 kilograms of crushing are mixed, and 2350 grams of wheat brans of addition,
459 grams of calcium chloride, 387 grams of frerrous chlorides, 589 grams of dipotassium hydrogen phosphates and 13 kg of water mix together, the fermentation after being mixed
Culture medium；Be added into fermentation medium 9 kilograms of bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor,
It stirs and is uniformly mixed；Fermentation medium after inoculation is divided in the fermentation tray with 75% alcohol surface sterilization, tray
Middle charging is with a thickness of 8 centimetres, the wet gauze of surface of material covering sterilization treatment in tray, then the solid that tray is placed in sterilizing is sent out
Shelf top fermentation in ferment room, control environment temperature are 60 DEG C, fermentation time 60h；After fermentation, it will be obtained after fermentation
Product after drying, crushing and packaging, obtained a kind of feeding antivirus somatotropic agent finished product of green.
Embodiment 3: by the high temperature resistant bacillus licheniformis of slant preservation (Bacillus licheniformis) HL-3 connects
After entering to sterilizing in cooling LB liquid medium, 12h is cultivated in 55 DEG C, 150r/min, is prepared into bacillus licheniformis
(Bacillus licheniformis) HL-3 seed liquor is spare.Fish meal processing waste water is concentrated by evaporation to protein content and is
18% is spare, and it is spare that Radix Glycyrrhizae and Radix Astragali are smashed it through 60 meshes；The fish meal processing waste water and 100 kilograms of powder that 80 kilograms are concentrated
Broken Chinese herbal medicine (+30 kilograms of Radix Astragalis of 70 kilograms of Radix Glycyrrhizaes) mixing, and 3980 grams of wheat brans, 499 grams of calcium chloride, 398 grams of chlorinations are added
Ferrous, 590 grams of dipotassium hydrogen phosphates and 16 kg of water mix together, the fermentation medium after being mixed；Into fermentation medium
Be added 9 kilograms of bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor, it stirs and is uniformly mixed；After inoculation
Fermentation medium be divided in the fermentation tray with 75% alcohol surface sterilization, in tray charging with a thickness of 6 centimetres, shallowly
The wet gauze of surface of material covering sterilization treatment in disk, then tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing,
Controlling environment temperature is 57 DEG C, fermentation time 56h；After fermentation, by the product obtained after fermentation through drying, crush and
After packaging, a kind of feeding antivirus somatotropic agent finished product of green has been obtained.
Claims (7)
1. a kind of method for preparing the feeding antivirus somatotropic agent of green using aquatic product protein and Chinese herbal medicine hot fermentation, feature exist
In: this method comprises the following steps:
(1) by the high temperature resistant bacillus licheniformis of slant preservation (Bacillus licheniformis) after HL-3 is linked into sterilizing
In cooling LB liquid medium, in 55 DEG C, 150r/min cultivate 12h, be prepared into bacillus licheniformis (Bacillus licheniformis) HL-3 seed liquor is spare；
(2) spare or aquatic products processing wastewater is concentrated by evaporation to protein content is after rubbing fresh low-value aquatic product protein
15%~20% is spare；
(3) that Chinese herbal medicine is smashed it through 60 meshes is spare；
(4) Chinese herbal medicine that the aquatic products processing wastewater and step (3) of the low-value aquatic product protein or concentration that rub step (2) crush
It is mixed in mass ratio for the ratio of 0.5 ~ 1.5:1；
(5) nutrients and water are added into the mixture that step (4) obtains, and is mixed, and obtain final moisture content control 40%
Fermentation medium in~80% range；
(6) accessed into the fermented and cultured that step (5) obtains bacillus licheniformis prepared by step (1) (Bacillus licheniformis) HL-3 seed liquor, and mix；
(7) fermentation of raw material: the fermentation medium for the addition bacterium that step (6) obtain is fermented in following manner: fermentation
Culture medium is divided in the fermentation tray sterilized, and charging is with a thickness of 4cm~8cm, and the covering of fermented and cultured primary surface is gone out in tray
The wet gauze of bacterium, then tray is placed in the shelf top fermentation in sterilized solid fermentation room, environment temperature is controlled 55 DEG C ~ 60
DEG C, fermentation time is 48h~60h；
(8) product handling system: fermentation ends have obtained a kind of green by the product obtained after fermentation after drying, crushing and packaging
The feeding antivirus somatotropic agent finished product of color.
High temperature resistant bacillus licheniformis used in the step (1) (Bacillus licheniformis) HL-3 is in Guangdong Province
The deposit number of Culture Collection is GDMCC No:60003.
2. a kind of utilization aquatic product protein according to claim 1 and Chinese herbal medicine hot fermentation prepare the feeding antibacterial growth-promoting of green
The method of long agent, it is characterised in that: in the step (2) low-value aquatic product protein be leftovers of tilapia, krill, shrimp head,
Scallop body, gadus leftover bits and pieces, squid leftover bits and pieces, Harengula zunasi leftover bits and pieces, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces
One of with stripped tuna leftover bits and pieces.
3. a kind of utilization aquatic product protein according to claim 1 and Chinese herbal medicine hot fermentation prepare the feeding antibacterial growth-promoting of green
The method of long agent, it is characterised in that: the aquatic products processing wastewater in the step (2) is fish meal processing waste water, chitin processing
One of alkali-wash waste water, prawn waste water from cooking and minced fillet processing waste water of process.
4. a kind of utilization aquatic product protein according to claim 1 and Chinese herbal medicine hot fermentation prepare the feeding antibacterial growth-promoting of green
The method of long agent, it is characterised in that: the Chinese herbal medicine in the step (3) is Radix Glycyrrhizae, Fructus Forsythiae, Asiatic plantain, perillaseed, Schisandra chinensis, horse
One of whip grass, Radix Astragali, honeysuckle, granatum, garden burnet, the coptis, cordate houttuynia, kuh-seng and smilax or in which two or more
Mixture.
5. a kind of utilization aquatic product protein according to claim 1 and Chinese herbal medicine hot fermentation prepare the feeding antibacterial growth-promoting of green
The method of long agent, it is characterised in that: the nutrients in the step (5) is wheat bran, calcium chloride, frerrous chloride and dipotassium hydrogen phosphate
Composed mixture.
6. a kind of utilization aquatic product protein according to claim 1 and Chinese herbal medicine hot fermentation prepare the feeding antibacterial growth-promoting of green
The method of long agent, it is characterised in that: the additional amount of the nutrients in the step (5) accounts for fermentation with the quality of the nutrients added
Culture medium gross mass percentages, are respectively as follows: wheat bran 0.01%~15%, calcium chloride 0.01%~5%, frerrous chloride 0.01%~5%,
Dipotassium hydrogen phosphate 0.01%~5%.
7. a kind of utilization aquatic product protein according to claim 1 and Chinese herbal medicine hot fermentation prepare the feeding antibacterial growth-promoting of green
The method of long agent, it is characterised in that: bacillus licheniformis in the step (6) (Bacillus licheniformis) HL-3
Inoculum concentration accounts for fermentation medium gross mass percentages to access the quality of seed liquor, is 1%~9%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610129696.7A CN105707473B (en) | 2016-03-09 | 2016-03-09 | A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610129696.7A CN105707473B (en) | 2016-03-09 | 2016-03-09 | A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105707473A CN105707473A (en) | 2016-06-29 |
| CN105707473B true CN105707473B (en) | 2019-08-13 |
Family
ID=56157396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610129696.7A Active CN105707473B (en) | 2016-03-09 | 2016-03-09 | A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105707473B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877257B (en) * | 2021-03-18 | 2023-04-07 | 昆明理工大学 | Bacillus licheniformis and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731453A (en) * | 2010-01-25 | 2010-06-16 | 陈忆凤 | Method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish |
| CN103027187A (en) * | 2013-01-05 | 2013-04-10 | 北京嘉博文生物科技有限公司 | Anti-stress fermentation protein feed and producing method thereof |
| CN104719635A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
-
2016
- 2016-03-09 CN CN201610129696.7A patent/CN105707473B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731453A (en) * | 2010-01-25 | 2010-06-16 | 陈忆凤 | Method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish |
| CN103027187A (en) * | 2013-01-05 | 2013-04-10 | 北京嘉博文生物科技有限公司 | Anti-stress fermentation protein feed and producing method thereof |
| CN104719635A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
Non-Patent Citations (1)
| Title |
|---|
| 一株地衣芽孢杆菌碱性蛋白酶的研究I;黄红英 等;《微生物学通报》;20011231;第28卷(第5期);第20-24页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105707473A (en) | 2016-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104041717B (en) | A kind of vinasse laying cycle of laying hens special feed processed and preparation method thereof | |
| CN105661013B (en) | A kind of method that fermentation of Chinese herbal medicine prepares compound feed additive for promoting growth | |
| CN103652452B (en) | A kind of prawn biological feedstuff and application thereof | |
| CN104719635A (en) | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine | |
| CN104000008A (en) | Protein feed raw material prepared from Asian carps and preparation method for protein feed raw material | |
| CN105685475A (en) | Method for preparing feed antibiotic substitute from subtilin producing bacillus subtilis fermented Chinese herbal medicine | |
| CN105661012B (en) | A kind of hot fermentation method of Chinese herbal medicine | |
| CN103859155A (en) | Refining method for probiotics-containing cassava feed | |
| CN105010748A (en) | Preparation method of mutton sheep feed and mutton sheep feed prepared by preparation method | |
| CN102987097A (en) | Method for producing complex enzyme preparation for feed by use of panax pseudoginseng slag fermentation | |
| CN104905041A (en) | Feeding-attractant high-efficiency pig feed and preparation method thereof | |
| CN106376723A (en) | Special feed for breeding broiler chickens | |
| CN105639209A (en) | Enteromorpha type stichopus japonicus biological feed and preparation method thereof | |
| CN104082528B (en) | A kind of feed addictive and preparation method thereof | |
| CN104686804B (en) | A method of activated peptide forage additive is prepared using aquatic product leftovers | |
| CN103892045A (en) | Livestock and poultry feed produced by sophora japonica solid dregs, and preparation method thereof | |
| CN105707473B (en) | A method of the feeding antivirus somatotropic agent of green is prepared using aquatic product protein and Chinese herbal medicine hot fermentation | |
| CN105746886B (en) | A method of compound green feed additive for promoting growth is prepared using aquatic product protein and Chinese herbal medicine fermentation | |
| CN105746889B (en) | A method of antibacterial green feed additive for promoting growth is prepared using aquatic product protein two-step fermentation | |
| CN103519028B (en) | A kind of broiler breeding feed and method for breeding thereof | |
| CN105724764B (en) | A method of antibacterial green feed additive for promoting growth is prepared using aquatic product protein fermentation | |
| CN1146865A (en) | Microecologic freshwater fish feed | |
| CN105746892B (en) | A method of green feed additive for promoting growth is prepared using aquatic product protein solid state fermentation | |
| CN105685478B (en) | A method of green feed additive for promoting growth is prepared using aquatic product protein hot fermentation | |
| CN105746891B (en) | A method of utilizing aquatic product protein and feather meal fermentation preparation fowl growth promotion small peptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |