CN103540554B - Post-sprayed micro-ecological preparation for expanded feeds, and preparation method and application of preparation - Google Patents
Post-sprayed micro-ecological preparation for expanded feeds, and preparation method and application of preparation Download PDFInfo
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- CN103540554B CN103540554B CN201310535869.1A CN201310535869A CN103540554B CN 103540554 B CN103540554 B CN 103540554B CN 201310535869 A CN201310535869 A CN 201310535869A CN 103540554 B CN103540554 B CN 103540554B
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Abstract
The invention discloses a post-sprayed micro-ecological preparation for expanded feeds, and a preparation method and application of the preparation. The post-sprayed micro-ecological preparation comprises the following components in percentage by mass: 40 to 60 percent of grease, 1 to 2 percent of solid micro-ecological preparation, 2 to 3 percent of emulsifying agent, 1.5 to 3 percent of protective agent, 0.1 to 0.3 percent of preservative and the balance of water. The preparation method comprises the following steps of dissolving the solid micro-ecological preparation into water, simultaneously adding the protective agent and the preservative, and uniformly stirring the components to obtain a liquid micro-ecological preparation; uniformly mixing the grease and the emulsifying agent while stirring, adding the liquid micro-ecological preparation, and performing homogenizing to completely and uniformly mix the components to obtain the post-sprayed micro-ecological preparation for the expanded feeds. The post-sprayed micro-ecological preparation can be directly sprayed onto the surfaces of the expanded feeds, can be stored for a long time, and is high in viable bacterium content and stable in activity, the shortcomings of high death rate of viable bacteria and high activity loss of a conventional liquid micro-ecological preparation are avoided, feed production procedures are simultaneously simplified, the feed production cost is lowered, and the quality of the feeds is improved.
Description
Technical field
The invention belongs to feed probiotics and Application Areas thereof, after particularly relating to a kind of expanded pellet diet, spray probiotics and preparation method thereof and application.
Background technology
Probiotics be one safely, have no side effect, noresidue, pollution-free, do not produce resistance, cost is low, easy to use, and can promote growth of animal, the new additive agent of control person poultry disease, substitute antibiotics.Feeding micro-ecological preparation arises at the historic moment based on the safety issue of feeding antibiotic, it can not only play restraining effect to pathogenic bacterium, the nontoxic and promotion growth of animals or poultry to livestock product, and livestock product meat flavor can be improved, being conducive to ecotope and the export trade, is the best solution of following green feed.
Current, during feed manufacturing preposition interpolation probiotics easily cause feed factory medicine class additive to the damage suppression of probiotics inactivation, therefore, in the technology of particle surface post vacuum spraying probiotics after the modified granulation of domestic and international employing feed, this technology effectively can improve the inside and outside homogeneity of granulated feed, and can improve probiotics viable bacteria storage rate.But, this spraying technology is for common liquid micro-ecological preparations, because probiotics needs to be sprayed on expanded rear granulated feed surface, so feed surface temperature and all easily cause thalline inactivation in follow-up packed and transported process, affect the Forage Nutritive Value of feed itself.
Summary of the invention
In order to overcome the shortcoming of prior art with not enough, primary and foremost purpose of the present invention sprays probiotics after being to provide a kind of expanded pellet diet.This rear spray probiotics preparation is simple, and stability is strong, and viable bacteria content is high, and the shelf time is long, easy to use.
The preparation method of probiotics is sprayed after another object of the present invention is to provide described expanded pellet diet.
Another object of the present invention sprays the application of probiotics after being to provide described expanded pellet diet.
Object of the present invention is achieved through the following technical solutions: spray probiotics after a kind of expanded pellet diet, comprises following component by mass percentage: grease 40 ~ 60%, solid probiotics 1 ~ 2%, emulsifying agent 2 ~ 3%, protective material 1.5 ~ 3%, sanitas 0.1 ~ 0.3%, water is surplus;
Described solid probiotics is preferably one in Bacillus coagulans, subtilis, Bacillus licheniformis and milk-acid bacteria or at least two kinds;
Described emulsifying agent is preferably castor oil phosphate ester;
Described protective material is preferably stearic acid and glycerol fatty acid ester, and wherein glycerol fatty acid ester is preferably the mixture of monoesters and diester mass ratio 1:1; Described stearic acid sprays 0.5 ~ 1% of probiotics total mass per-cent after being preferably expanded pellet diet; Described glycerol fatty acid ester sprays 1 ~ 2% of probiotics total mass per-cent after being preferably expanded pellet diet;
Described sanitas is preferably Sorbic Acid.
Spray the preparation method of probiotics after described expanded pellet diet, comprise following concrete steps:
(1) grease of mass percent 40 ~ 60%, the solid probiotics of mass percent 1 ~ 2%, the emulsifying agent of mass percent 2 ~ 3%, the protective material of mass percent 1.5 ~ 3%, the sanitas of mass percent 0.1 ~ 0.3% and the water of surplus is got; By water-soluble for solid probiotics, and then add protective material and sanitas simultaneously, each component is stirred, obtains liquid micro-ecological preparations;
(2) by grease and emulsifying agent mixing and stirring, add the liquid micro-ecological preparations prepared in step (1), through homogeneous, each component is fully mixed, after obtaining expanded pellet diet, sprays probiotics;
Stirring described in step (1) and step (2) is preferably 60r/min at rotating speed, and model is stir under YRTZ concentric double-shaft stirrer;
Homogeneous described in step (2) preferably uses the clarifixator homogeneous 3 ~ 5 minutes that model is JJ2/60.
Spray probiotics after described expanded pellet diet and prepare the application in expanded pellet diet, preferably comprise following steps: add in rear spray storage tank by spraying probiotics after the expanded pellet diet prepared, Direct spraying, in the surface of expanded pellet diet, obtains the expanded pellet diet adding rear spray probiotics.
It is 5 × 10 that the expanded pellet diet that probiotics is sprayed in described interpolation is afterwards preferably expanded pellet diet per ton spraying 10 ~ 30kg bacterial content
9~ 1 × 10
11probiotics is sprayed after the expanded pellet diet of cfu/g.
The present invention compared with prior art has following advantages and effect:
(1) premium propertiess such as the emulsifying agent ricinoleate ester that the present invention adopts has that surface tension is low, emulsifying property good, nontoxic, nonirritant, electrolyte-resistant.This emulsifying agent belongs to water-in-oil-type, emulsified fat, makes it fully mix with probiotics solution, plays the effect of protection probiotics, and probiotics can be made to drop into indiffusion loss in pond, can be fully utilized.
(2) the present invention adopts stearic acid and glycerol fatty acid ester as protective material, and protection probiotics is activity stabilized, exempts to be affected by the external environment.Adopt Sorbic Acid as sanitas, can high-effect anti-mould, anticorrosion, can feed nutritive value be increased simultaneously, improve albumen in feed, promote the effects such as aquatic animal to grow.
(3) rear spray probiotics of the present invention can in rear spray storage tank the long period preserve, activity stabilized, viable bacteria content is high.This method is applicable to all types probiotics, and it is convenient therefore can to bring to fodder production, can be applied to production in enormous quantities simultaneously.
(4) spray probiotics after the present invention and be directed to expanded pellet diet, can Direct spraying its surface, and can preserve for a long time, viable bacteria content is high, activity stabilized, avoids liquid micro-ecological preparations viable bacteria in the past easily dead, the drawback that loss of activity is larger, simplify fodder production operation simultaneously, reduce fodder production cost, improve feeding quality.
(5) rear spray probiotics of the present invention ensure that viable bacteria content, improve the digestibility of feed, reduce feed coefficient, improve aquatic animal to grow speed, improve animal body immunologic function, improve breeding environment, thus reach volume increase, the effect reducing labor force and reduce costs, for breeding production brings economic benefit.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1:
Following composition is by mass percentage:
(1) above component is by mass percentage got; By water-soluble for Bacillus coagulans (buying from Bo Shiao bio tech ltd, the Sanmenxia Gorge), and then add stearic acid simultaneously, glycerol fatty acid ester (buying from Jinan Dongrun Fine Chemical Technology Co., Ltd.), Sorbic Acid, be 60r/min by each component at rotating speed, model is stir under YRTZ concentric double-shaft stirrer, obtains liquid condensation bacillus preparation.
(2) grease is mixed with emulsifying agent castor oil phosphate ester, be 60r/min at rotating speed, model is stir under YRTZ concentric double-shaft stirrer, add the liquid condensation bacillus preparation prepared in step (1), through the clarifixator homogeneous 3 minutes that model is JJ2/60, each component is fully mixed, after obtaining expanded pellet diet, sprays coagulated bacillus living formulation.
(3), after homogeneous, add rear spray storage tank directly use spraying coagulated bacillus living formulation after the expanded pellet diet prepared.
Embodiment 2:
Following composition is by mass percentage:
(1) above component is by mass percentage got; By subtilis (buying from Bo Shiao bio tech ltd, the Sanmenxia Gorge) and milk-acid bacteria (buying from Bo Shiao bio tech ltd, the Sanmenxia Gorge) water-soluble, and then add stearic acid simultaneously, glycerol fatty acid ester (buying from Jinan Dongrun Fine Chemical Technology Co., Ltd.), Sorbic Acid, be 60r/min by each component at rotating speed, model is stir under YRTZ concentric double-shaft stirrer, obtains liquid subtilis and lactic acid bacteria formulation.
(2) grease is mixed with emulsifying agent castor oil phosphate ester, be 60r/min at rotating speed, model is stir under YRTZ concentric double-shaft stirrer, add the liquid subtilis and lactic acid bacteria formulation that prepare in step (1), through the clarifixator homogeneous 5 minutes that model is JJ2/60, each component is fully mixed, each component is fully mixed, after obtaining expanded pellet diet, spray subtilis and viable lactic acid bacteria preparation.
(3), after homogeneous, add rear spray storage tank directly use spraying subtilis and viable lactic acid bacteria preparation after the expanded pellet diet prepared.
Embodiment 3:
Following composition is by mass percentage:
(1) above component is by mass percentage got; By subtilis (buying from Bo Shiao bio tech ltd, the Sanmenxia Gorge) and Bacillus licheniformis (buying from Bo Shiao bio tech ltd, the Sanmenxia Gorge) water-soluble, and then add stearic acid simultaneously, glycerol fatty acid ester (buying from Jinan Dongrun Fine Chemical Technology Co., Ltd.), Sorbic Acid, be 60r/min by each component at rotating speed, model is stir under YRTZ concentric double-shaft stirrer, obtains liquid subtilis and Bacillus licheniformis preparation.
(2) grease is mixed with emulsifying agent castor oil phosphate ester, be 60r/min at rotating speed, model is stir under YRTZ concentric double-shaft stirrer, add the liquid subtilis and Bacillus licheniformis preparation that prepare in step (1), through the clarifixator homogeneous 5 minutes that model is JJ2/60, each component is fully mixed, after obtaining expanded pellet diet, sprays subtilis and bacillus cicheniformis.
(3), after homogeneous, add rear spray storage tank directly use spraying subtilis and bacillus cicheniformis after the expanded pellet diet prepared.
Embodiment 4
Produce a collection of rear spray probiotics according to the preparation method of embodiment 1, Bacillus coagulans content is 1 × 10
11cfu/g.Spray coagulated bacillus living formulation 1 ton after producing expanded pellet diet according to the preparation method of embodiment 1, spray coagulated bacillus living formulation amount, viable bacteria content in period sampling measuring feed by after expanded pellet diet spraying 30kg per ton, and carried out culture experiment.Test fish is Sarotherodon sp, and mean body weight is 34.50g.Test design does not add the expanded pellet diet of probiotics for contrast A group, and (common probiotics is that Bacillus coagulans is water-soluble, and making bacterial content is 1 × 10 to add common probiotics
11the Bacillus coagulans preparation of cfu/g; Bacillus coagulans, buys from Bo Shiao bio tech ltd, the Sanmenxia Gorge) expanded pellet diet be contrast B group, after adding, the expanded pellet diet of spray coagulated bacillus living formulation is test C group.Establish three test group altogether, often organize 3 repetitions, each repetition 25 tail.Test conditions: test adopts hydrostatic to cultivate with the exchange avoiding water between each case.Rate of throwing something and feeding feed day is by 3% of body weight, and divide and make three equal parts in 8:00 every day, 11:00,17:00 throw something and feed.Between feeding period, water temperature is 26 ~ 33 DEG C, pH(8.0 ± 0.2), dissolved oxygen 4.0 ~ 6.0mg/L, respectively measures a water temperature morning and afternoon every day, measures a water quality every two weeks.Breeding cycle 8 weeks.Test-results finds, the rear spray coagulated bacillus living formulation shelf time is longer, and viable bacteria content is high, compared with common Bacillus coagulans preparation activity stabilized (table 4).Use the expanded pellet diet of rear spray coagulated bacillus living formulation of the present invention compared with conventional expanded pellet diet, rear spray coagulated bacillus living formulation has remarkably influenced to Sarotherodon sp digestibility and growth, as shown in table 1, test group apparent digestibility of the dry matter improves 10.68% respectively compared with control group and common Bacillus coagulans group, 10.07%(P < 0.05), protein apparent digestibility improves 4.71% compared with control group and common Bacillus coagulans group respectively, 3.65%(P < 0.05), rear spray coagulated bacillus living formulation of the present invention also makes Sarotherodon sp rate of body weight gain improve 10.76% compared with control group and common Bacillus coagulans group simultaneously, 8.56%(P < 0.05), feed coefficient reduces 9.2%, 5.0%(P < 0.05).In addition, compared with conventional feed of throwing something and feeding, after using the present invention, spray the utilization ratio that probiotics directly can improve feedstuff raw material, reduce feed cost, for aquaculture brings economic benefit.
Table 1 Sarotherodon sp growth indexes changes
Embodiment 5
Produce a collection of rear spray probiotics according to the preparation method of embodiment 2, subtilis and milk-acid bacteria are used in test, and bacillus subtilis bacterial content is 1 × 10
11cfu/g, content of lactic acid bacteria is 5 × 10
9cfu/g.Spray subtilis and 1 ton, viable lactic acid bacteria preparation after producing expanded pellet diet according to the preparation method of embodiment 2, spray subtilis and viable lactic acid bacteria amount of formulation, viable bacteria content in period sampling measuring feed by after expanded pellet diet spraying 10kg per ton.And carry out hybridized prussian carp culture experiment, test carries out 50 days.Test conditions: adopt continuous charge oxygenation, the water of purification system filters through precipitation, sand filter, to remove solid waste.Duration of test adopt natural lighting, the biochemical condition of breeding water is respectively: water temperature (22 ± 2.0) DEG C, oxyty > 6mg/L, total ammonia nitrogen in optimum range, pH(8.0 ± 0.2).The expanded pellet diet that test design does not add probiotics is contrast A group, add common probiotics (common probiotics be by subtilis and milk-acid bacteria water-soluble, make bacillus subtilis bacterial content 1 × 10
11cfu/g, content of lactic acid bacteria is 5 × 10
9the subtilis of cfu/g and lactic acid bacteria formulation; Subtilis and milk-acid bacteria, all buy from Bo Shiao bio tech ltd, the Sanmenxia Gorge) expanded pellet diet be contrast B group, after adding, the expanded pellet diet of spray subtilis and viable lactic acid bacteria preparation is test C group.Establish three test group altogether, often organize 12 repetitions, each repetition 25 tail.Test-results finds, rear spray subtilis and the viable lactic acid bacteria preparation shelf time longer, viable bacteria content is compared activity stabilized (table 4) with lactic acid bacteria formulation by high and common subtilis.Test-results is shown in Table 2, can show that rear spray subtilis and viable lactic acid bacteria preparation have certain growth promoting function to hybridized prussian carp by table 2, test C group compares with other group and is the highest in rate of body weight gain and net gain two, is 63.6% respectively, 13.07g.And test group feed coefficient is starkly lower than control group, there is significant difference, after illustrating, spray probiotics is conducive to the growth of fish, after this coordinates probiotics to enter enteron aisle, sporeformer can produce bacteriocin and can antagonism pathogenic bacterium, consumes oxygen in enteron aisle, produces organic acid and is conducive to lactobacter growth and surely grows, maintain the eubiosis, the various enzymes such as sporeformer product proteolytic enzyme, lipase, amylase contribute to enteron aisle and digest and assimilate, and improve efficiency of feed utilization, growth promoting effects.
Rate of body weight gain (%)=(test whole fish to weigh-test beginning fish is heavy)/test beginning fish heavy × 100.
Feed coefficient=food ration/test whole fish to weigh-test beginning fish is heavy.
Hybridized prussian carp production performance situation tested by table 2
| Index | A group | B group | C group |
| Initial tail all (g) | 20.56 | 20.75 | 20.55 |
| Final tail all (g) | 33.12 | 33.26 | 34.62 |
| Net gain (g) | 12.56 | 12.51 | 13.07 |
| Rate of body weight gain (%) | 61.13 | 60.34 | 63.60 |
| Feed consumption (g) | 28.35 | 28.06 | 26.90 |
| Feed coefficient | 2.26 | 2.20 | 2.06 |
Embodiment 6
Produce a collection of rear spray probiotics according to the preparation method of embodiment 3, bacillus subtilis bacterial content is 1 × 10
11cfu/g, Bacillus licheniformis content is 5 × 10
10cfu/g.Subtilis and bacillus cicheniformis 500kg is sprayed after producing expanded pellet diet according to the preparation method of embodiment 3, subtilis and bacillus cicheniformis amount is sprayed by after expanded pellet diet spraying 20kg per ton, viable bacteria content in period sampling measuring feed, and carried out culture experiment.The average cabrage 50.23g of test carp, the long 8.5cm of average body, test schedules to last 100d.Carp is supported in 6m × 2m × 2m concrete fishpond, and 46 tails are thrown in every pond at random.Every day, fixed point threw in bait, within first 50 days, threw in bait 4 times, within latter 50 days, threw in bait 3 times.Duration of test water temperature is 16.8 ~ 27.5 DEG C, pH6.0 ~ 7.0, dissolved oxygen 3.0 ~ 8.0mg/L.The expanded pellet diet that test design does not add probiotics is contrast A group, add common probiotics (common probiotics be by subtilis and Bacillus licheniformis water-soluble, make bacillus subtilis bacterial content 1 × 10
11cfu/g, Bacillus licheniformis content is 5 × 10
10the subtilis of cfu/g and Bacillus licheniformis preparation; Subtilis and Bacillus licheniformis, all buy from Bo Shiao bio tech ltd, the Sanmenxia Gorge) expanded pellet diet be contrast B group, after adding, the expanded pellet diet of spray subtilis and bacillus cicheniformis is test C group.Establish three test group altogether, often organize 3 repetitions, each repetition 46 tail.Test-results shows, rear spray subtilis and the Bacillus licheniformis viable bacteria shelf time longer, viable bacteria content is high, compared with common probiotics activity stabilized (table 4).Use the expanded pellet diet of rear spray probiotics of the present invention compared with conventional expanded pellet diet, rear spray probiotics improves 11.8% to carp weightening finish, and feed coefficient reduces 0.24 than control group, and the weightening finish of test C group is apparently higher than contrast A group.
After table 3, spray probiotics is to carp growth promoting function
The change (%) of expanded pellet diet probiotics viable bacteria rate with the shelf time is sprayed after table 4 feed factory
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (9)
1. spray a probiotics after expanded pellet diet, it is characterized in that comprising following component by mass percentage: grease 40 ~ 60%, solid probiotics 1 ~ 2%, emulsifying agent 2 ~ 3%, protective material 1.5 ~ 3%, sanitas 0.1 ~ 0.3%, water is surplus;
Described emulsifying agent is castor oil phosphate ester;
Described protective material is stearic acid and glycerol fatty acid ester.
2. spray probiotics after expanded pellet diet according to claim 1, it is characterized in that: described solid probiotics is one in Bacillus coagulans, subtilis, Bacillus licheniformis and milk-acid bacteria or at least two kinds.
3. spray probiotics after expanded pellet diet according to claim 1, it is characterized in that:
Described sanitas is Sorbic Acid.
4. spray probiotics after expanded pellet diet according to claim 1, it is characterized in that: described stearic acid is spray 0.5 ~ 1% of probiotics total mass per-cent after expanded pellet diet;
Described glycerol fatty acid ester is the mixture of monoesters and diester mass ratio 1:1;
Described glycerol fatty acid ester is spray 1 ~ 2% of probiotics total mass per-cent after expanded pellet diet.
5. spray the preparation method of probiotics after the expanded pellet diet according to any one of Claims 1 to 4, it is characterized in that comprising following concrete steps:
(1) grease of mass percent 40 ~ 60%, the solid probiotics of mass percent 1 ~ 2%, the emulsifying agent of mass percent 2 ~ 3%, the protective material of mass percent 1.5 ~ 3%, the sanitas of mass percent 0.1 ~ 0.3% and the water of surplus is got; By water-soluble for solid probiotics, and then add protective material, sanitas simultaneously, each component is stirred, obtains liquid micro-ecological preparations;
(2) by grease and emulsifying agent mixing and stirring, add the liquid micro-ecological preparations prepared in step (1), through homogeneous, each component is fully mixed, after obtaining expanded pellet diet, sprays probiotics.
6. after expanded pellet diet according to claim 5, spray the preparation method of probiotics, it is characterized in that: stirring described in step (1) and step (2) is 60r/min at rotating speed, and model is stir under YRTZ concentric double-shaft stirrer;
The clarifixator homogeneous that homogeneous described in step (2) is use model is JJ2/60 3 ~ 5 minutes.
7. spray probiotics after the expanded pellet diet described in any one of Claims 1 to 4 and prepare the application in expanded pellet diet.
8. spray probiotics after expanded pellet diet according to claim 7 and prepare the application in expanded pellet diet, it is characterized in that comprising following steps: add in rear spray storage tank by spraying probiotics after the expanded pellet diet prepared, Direct spraying, in the surface of expanded pellet diet, obtains the expanded pellet diet adding rear spray probiotics.
9. spray probiotics after expanded pellet diet according to claim 8 and preparing the application in expanded pellet diet, it is characterized in that: the expanded pellet diet that probiotics is sprayed in described interpolation is afterwards expanded pellet diet per ton spraying 10 ~ 30kg bacterial content is 5 × 10
9~ 1 × 10
11probiotics is sprayed after the expanded pellet diet of cfu/g.
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| CN111607542B (en) * | 2020-06-11 | 2022-07-26 | 武汉市农业科学院 | Microecological preparation and preparation method and application thereof |
| CN112262918A (en) * | 2020-11-18 | 2021-01-26 | 内蒙古自治区农牧业科学院 | Feed microecological bactericide suspending agent and preparation method thereof |
| CN117770351A (en) * | 2024-02-23 | 2024-03-29 | 四川弘农科技有限责任公司 | Composite microbial fermented feed and preparation process thereof |
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Effective date of registration: 20220825 Address after: 550200 In Zhazuo Industrial Park, Xiuwen County, Guiyang City, Guizhou Province (in Qianxiao Demonstration Park, Jiuchang Town, Xiuwen County) Patentee after: Guizhou Chuanpai Feed Co.,Ltd. Address before: Room C2-1103, Innovation Building, No. 182, Science Avenue, High-tech Industrial Development Zone, Guangzhou, Guangdong, 510663 Patentee before: GUANGZHOU BOSAR BIOLOGICAL TECHNOLOGY CO.,LTD. Patentee before: YUNNAN BOSAR BIOTECHNOLOGY CO.,LTD. |
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