CN105851479A - Earthworm fermentation liquid, and preparation method and application method thereof - Google Patents

Earthworm fermentation liquid, and preparation method and application method thereof Download PDF

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Publication number
CN105851479A
CN105851479A CN201610258340.3A CN201610258340A CN105851479A CN 105851479 A CN105851479 A CN 105851479A CN 201610258340 A CN201610258340 A CN 201610258340A CN 105851479 A CN105851479 A CN 105851479A
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Prior art keywords
lumbricus
fermentation liquid
bran
fermentation
water
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CN201610258340.3A
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Chinese (zh)
Inventor
张奎
温志远
高桂芹
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Zhongrun Fenghe Feed Co Ltd
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Zhongrun Fenghe Feed Co Ltd
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Priority to CN201610258340.3A priority Critical patent/CN105851479A/en
Publication of CN105851479A publication Critical patent/CN105851479A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225Lactobacillus
    • C12R2001/23Lactobacillus acidophilus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention relates to fermentation liquid, and a preparation method and application method thereof, and belongs to the technical field of feed additives. The earthworm fermentation liquid has the technical scheme that the pH value of the earthworm fermentation liquid is 3.5 to 5.00; the protein content is higher than or equal to 26 percent. The preparation method comprises the following steps of (1) putting water into a fermentation tank; raising the temperature to 100 DEG C for sterilization and disinfection; after the water temperature is lowered to 38 DEG C +/- 1 DEG C, firstly adding lactobacillus acidophilus, and then adding beef extracts, peptone, sodium chloride and agaragar; (2) cutting up earthworms, performing uniform stirring, adding the materials into the fermentation tank according to the water mass percentage being 325 percent, maintaining the temperature at 38 DEG C +/- 1 DEG C, performing filtering after the constant temperature maintaining for 70 to 75 hours to obtain the earthworm fermentation liquid; (3) adding the earthworm fermentation liquid into bran, adding soybean molasses into a mixture obtained after the uniform mixing, obtaining a feed additive raw material; performing anaerobic fermentation for 70 to 74 hours to obtain the feed additive. The earthworm fermentation liquid, the preparation method and the application provided by the invention have the beneficial effects that the manufacturing process is simple; the weight increase rate of the livestock and poultry is improved by 25 percent; the feed conversion rate is improved by 21 percent.

Description

A kind of Lumbricus fermentation liquid and its preparation method and application method
Technical field
The present invention relates to the preparation method of a kind of Lumbricus fermentation liquid and its preparation method and application the livestock and poultry animal feed additives such as method, especially cattle, sheep, chicken, pig, belong to additive for farm animal feed preparing technical field.
Background technology
In recent years, protein feeds and new additive agent is produced by biotechnology particularly microbial fermentation technology exploitation novel fodder resource, increasingly it is subject to people's attention, after 21 century, the use of the feed products such as the forage protein enzyme preparation amino acid, vitamine antibiotic and the probiotic bacteria that utilize micro-organisms, makes fermentation engineering obtain wider application in feed industry.The aminoacid easily lacked in conventional feed can be made up, and other roughage material nutrient components can be made to convert rapidly, reach enhancing and digest and absorb effect.The problem of feed resource shortage governs the development of China's animal husbandry for a long time, and especially the wretched insufficiency of protein feeds, has become as global problem.Development high-efficiency feed industry, improving the grain transformation efficiency to livestock products and efficiency of feed utilization, exploitation novel fodder raw material is the optimal path meeting the people to the increasing demand of meat, fowl, fish, egg.Develop novel fodder resource, production protein feeds and new additive agent with biotechnology particularly microbial fermentation technology to be increasingly subject to people's attention.
At present, it is quite varied that fermentative feedstuff of microbe has been applied, and is broadly divided into 4 big classes: one is solid fermentation feedstuff, it is simply that utilize Fermentation to change the physicochemical character of feedstuff, or improve digestibility, extend period of storage.Or turn waste into wealth, not plump shell residue is become feedstuff;Or removing toxic substances detoxification, toxic cake is changed into the feedstuff of nontoxic low toxicity, this class fermented feed includes ensiling microbial roughage and basidiomycetes fermented livestock excrement and animality leftover bits and pieces fermentation puffed soybean fermentation detoxified feed and solid-state thalline fermentation protein feedstuff;Two is to utilize the thalline of microorganism raised growth breeding in liquid matrix and produce single cell protein such as yeast feed antibacterial feedstuff and bacterium SCP body protein, such as filamentous fungi thalline edible fungi mycelium and MBP, photosynthetic bacteria miniature algae feedstuff etc.;Three is the microbial project utilizing modernization, and fermentation accumulates the useful mesostate of microorganism or special metabolite, produces feeding aminoacidase preparation and antibiotic vitamin etc. with this;Four is to cultivate breeding the most feeding microorganism to prepare active bacteria formulation, also known as microbial ecological agent probiotics etc., modal fermentation raw material includes potato class, seed class, bran, the slag dregs of rice, potato seed slag, Semen Maydis grit, foot powder, citrus pulp, megasse, some grass meal, puffed soybean (such as cottonseed cake, rapeseed cake oilseeds, Semen Ricini cake etc.), stalk, feces and tankage etc..The shortcomings such as it is low that feedstuff prepared by said method exists conversion ratio, and the cycle is long, and protection effect is the most notable.
Summary of the invention
It is an object of the present invention to provide a kind of Lumbricus fermentation liquid and its preparation method and application method, there is higher nutritive value and medical value, improve poultry growth rate and feed conversion rate, solve the problems referred to above that background technology exists.
The technical scheme is that:
A kind of Lumbricus fermentation liquid, pH value 3.5-5.00, protein >=26%, viable bacteria 2,000,000,000 strain/gram, this Lumbricus fermentation liquid uses following processing step to prepare:
(1) breeding species is bacillus acidophilus, viable bacteria 2,000,000,000 strain/gram;
(2) water is put into fermentation tank and be heated up to 100 ° of C sterilizations, when water temperature is down to 38 ° of C ± 1 ° C, it is firstly added bacillus acidophilus, being subsequently adding Carnis Bovis seu Bubali cream, peptone, sodium chloride, agar, additional proportion is: every 100ml water adds bacillus acidophilus 10, Carnis Bovis seu Bubali cream 0.3g, peptone 1g, sodium chloride 0.5g, agar 2g;
(3) after Stain activation, Lumbricus chopping being stirred, add fermentation tank by the mass percent of water 25%, temperature keeps 38 ± 1 ° of C, and constant temperature is filtrated to get Lumbricus fermentation liquid after 70-75 hour;
Above-mentioned Lumbricus fermentation liquid, in described processing step (3): impurity in Lumbricus and Stomach contents less than Lumbricus gross mass≤1%.
The preparation method of a kind of Lumbricus fermentation liquid, comprises following processing step:
(1) breeding species is bacillus acidophilus, viable bacteria 2,000,000,000 strain/gram;
(2) water is put into fermentation tank and be heated up to 100 ° of C sterilizations, when water temperature is down to 38 ° of C ± 1 ° C, it is firstly added bacillus acidophilus, being subsequently adding Carnis Bovis seu Bubali cream, peptone, sodium chloride, agar, additional proportion is: every 100ml water adds bacillus acidophilus 10, Carnis Bovis seu Bubali cream 0.3g, peptone 1g, sodium chloride 0.5g, agar 2g;
(3), after Stain activation, Lumbricus chopping is stirred, adds fermentation tank by the mass percent of water 25%, temperature keeps 38 ± 1 ° of C, and constant temperature is filtrated to get Lumbricus fermentation liquid after 70-75 hour, this Lumbricus fermentation liquid: pH value 3.5-5.00, protein >=26%, viable bacteria 2,000,000,000 strain/gram.
The preparation method of above-mentioned Lumbricus fermentation liquid, in described processing step (3): impurity in Lumbricus and Stomach contents less than Lumbricus gross mass≤1%.
A kind of application process of Lumbricus fermentation liquid, above-mentioned Lumbricus fermentation liquid is added in bran bran, additional proportion mass percent is the 1-1.5% of bran bran gross mass, mixture after mix homogeneously adds soy molasses, additional proportion mass percent is the 15-20% of mixture gross mass, obtains raw materials of feed additives;Raw materials of feed additives is carried out anaerobic fermentation, 35-38 ° of C of constant temperature, ferments and obtain feed additive in 70-74 hour.
In the application process of above-mentioned Lumbricus fermentation liquid, Lumbricus fermentation liquid adds mass percent is bran bran gross mass the 1% of bran bran.
In the application process of above-mentioned Lumbricus fermentation liquid, Lumbricus fermentation liquid adds mass percent is bran bran gross mass the 1.5% of bran bran.
Utilize the feed additive that Lumbricus fermentation liquid prepares, biological containing sudismase, cellulase, protease, Lumbrukinase, fibrinolysin etc. and numerous undeveloped growth promoting factor, there is higher nutritive value and medical value, can add in the feedstuff of the livestock and poultry animal material such as cattle, sheep, chicken, pig, breeding performonce fo animals can be promoted, improve animal body immunity.
Beneficial effects of the present invention: Lumbricus fermentation liquid has a characteristic that (1) fragrant odour, nutritious, good palatability, digestive utilization ratio is high;(2) resolve into little molecular nutrition material, during absorption, save digestible energy;(3) immunocompetence and resistance against diseases are strengthened;(4) feedstuff is acidity, and the processing technology of safety (5) the Lumbricus fermentation liquid that improve feedstuff is simple, low cost, (6) feed additive that Lumbricus fermentation liquid prepares is used, in growth of animals or poultry performance compared with common additives, improve poultry growth rate 25%, improve food conversion ratio 21%.
Detailed description of the invention
The present invention will be further described by the following examples.
A kind of Lumbricus fermentation liquid, pH value 3.5-5.00, protein >=26%, viable bacteria 2,000,000,000 strain/gram, this Lumbricus fermentation liquid uses following processing step to prepare:
(1) breeding species is bacillus acidophilus, viable bacteria 2,000,000,000 strain/gram;
(2) water is put into fermentation tank and be heated up to 100 ° of C sterilizations, when water temperature is down to 38 ° of C ± 1 ° C, it is firstly added bacillus acidophilus, being subsequently adding Carnis Bovis seu Bubali cream, peptone, sodium chloride, agar, additional proportion is: every 100ml water adds bacillus acidophilus 10, Carnis Bovis seu Bubali cream 0.3g, peptone 1g, sodium chloride 0.5g, agar 2g;
(3) after Stain activation, Lumbricus chopping being stirred, add fermentation tank by the mass percent of water 25%, temperature keeps 38 ± 1 ° of C, and constant temperature is filtrated to get Lumbricus fermentation liquid after 70-75 hour;
The described impurity in Lumbricus and Stomach contents less than Lumbricus gross mass≤1%.
The preparation method of a kind of Lumbricus fermentation liquid, comprises following processing step:
(1) breeding species is bacillus acidophilus, viable bacteria 2,000,000,000 strain/gram;
(2) water is put into fermentation tank and be heated up to 100 ° of C sterilizations, when water temperature is down to 38 ° of C ± 1 ° C, it is firstly added bacillus acidophilus, being subsequently adding Carnis Bovis seu Bubali cream, peptone, sodium chloride, agar, additional proportion is: every 100ml water adds bacillus acidophilus 10, Carnis Bovis seu Bubali cream 0.3g, peptone 1g, sodium chloride 0.5g, agar 2g;
(3), after Stain activation, Lumbricus chopping is stirred, adds fermentation tank by the mass percent of water 25%, temperature keeps 38 ± 1 ° of C, and constant temperature is filtrated to get Lumbricus fermentation liquid after 70-75 hour, this Lumbricus fermentation liquid: pH value 3.5-5.00, protein >=26%, viable bacteria 2,000,000,000 strain/gram.
The described impurity in Lumbricus and Stomach contents less than Lumbricus gross mass≤1%.
A kind of application process of Lumbricus fermentation liquid, use above-mentioned limited Lumbricus fermentation liquid, Lumbricus fermentation liquid is added in bran bran, additional proportion mass percent is the 1-1.5% of bran bran gross mass, mixture after mix homogeneously adds soy molasses, additional proportion mass percent is the 15-20% of mixture gross mass, obtains raw materials of feed additives;Raw materials of feed additives is carried out anaerobic fermentation, 35-38 ° of C of constant temperature, ferments and obtain feed additive in 70-74 hour.
In the present embodiment, when making the feed additive of cattle or sheep, Lumbricus fermentation liquid adds mass percent is bran bran gross mass the 1% of bran bran.The usage amount of this feed additive is: when feeding cattle, add the 5-10% that mass percent is cattle feedstuff gross mass of this feed additive in cattle feedstuff;When feeding sheep, sheep feedstuff adds mass percent is sheep feedstuff gross mass the 5% of this feed additive.
When making the feed additive of pig or chicken, Lumbricus fermentation liquid adds mass percent is bran bran gross mass the 1.5% of bran bran.The usage amount of this feed additive is: when feeding pigs, and adds the 4-8% that mass percent is pig feed gross mass of this feed additive in pig feed;When feeding chicken, chicken feed adds the 3-5% that mass percent is sheep feedstuff gross mass of this feed additive.

Claims (7)

1. a Lumbricus fermentation liquid, it is characterised in that: pH value 3.5-5.00, protein >=26%, viable bacteria 2,000,000,000 strain/gram, this Lumbricus fermentation liquid uses following processing step to prepare:
(1) breeding species is bacillus acidophilus, viable bacteria 2,000,000,000 strain/gram;
(2) water is put into fermentation tank and be heated up to 100 ° of C sterilizations, when water temperature is down to 38 ° of C ± 1 ° C, it is firstly added bacillus acidophilus, being subsequently adding Carnis Bovis seu Bubali cream, peptone, sodium chloride, agar, additional proportion is: every 100ml water adds bacillus acidophilus 10, Carnis Bovis seu Bubali cream 0.3g, peptone 1g, sodium chloride 0.5g, agar 2g;
(3) after Stain activation, Lumbricus chopping being stirred, add fermentation tank by the mass percent of water 25%, temperature keeps 38 ± 1 ° of C, and constant temperature is filtrated to get Lumbricus fermentation liquid after 70-75 hour.
2. according to the Lumbricus fermentation liquid described in claims 1, it is characterised in that: in described processing step (3): impurity in Lumbricus and Stomach contents less than Lumbricus gross mass≤1%.
3. the preparation method of a Lumbricus fermentation liquid, it is characterised in that: comprise following processing step:
(1) breeding species is bacillus acidophilus, viable bacteria 2,000,000,000 strain/gram;
(2) water is put into fermentation tank and be heated up to 100 ° of C sterilizations, when water temperature is down to 38 ° of C ± 1 ° C, it is firstly added bacillus acidophilus, being subsequently adding Carnis Bovis seu Bubali cream, peptone, sodium chloride, agar, additional proportion is: every 100ml water adds bacillus acidophilus 10, Carnis Bovis seu Bubali cream 0.3g, peptone 1g, sodium chloride 0.5g, agar 2g;
(3), after Stain activation, Lumbricus chopping is stirred, adds fermentation tank by the mass percent of water 25%, temperature keeps 38 ± 1 ° of C, and constant temperature is filtrated to get Lumbricus fermentation liquid after 70-75 hour, this Lumbricus fermentation liquid: pH value 3.5-5.00, protein >=26%, viable bacteria 2,000,000,000 strain/gram.
4. according to the preparation method of the Lumbricus fermentation liquid described in claims 3, it is characterised in that: in described processing step (3): impurity in Lumbricus and Stomach contents less than Lumbricus gross mass≤1%.
5. the application process of a Lumbricus fermentation liquid, it is characterized in that: use the Lumbricus fermentation liquid that claims 1 are limited, Lumbricus fermentation liquid is added in bran bran, additional proportion mass percent is the 1-1.5% of bran bran gross mass, mixture after mix homogeneously adds soy molasses, additional proportion mass percent is the 15-20% of mixture gross mass, obtains raw materials of feed additives;Raw materials of feed additives is carried out anaerobic fermentation, 35-38 ° of C of constant temperature, ferments and obtain feed additive in 70-74 hour.
The application process of Lumbricus fermentation liquid the most according to claim 5, it is characterised in that: Lumbricus fermentation liquid adds mass percent is bran bran gross mass the 1% of bran bran.
The application process of Lumbricus fermentation liquid the most according to claim 5, it is characterised in that: Lumbricus fermentation liquid adds mass percent is bran bran gross mass the 1.5% of bran bran.
CN201610258340.3A 2016-04-25 2016-04-25 Earthworm fermentation liquid, and preparation method and application method thereof Pending CN105851479A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106470692A (en) * 2014-03-29 2017-03-01 国立大学法人新泻大学 Suppress compositionss and its manufacture method of the fatty liver and renal hypertrophy being caused by diabetes
CN106810399A (en) * 2017-02-16 2017-06-09 宁夏大学 The preparation method of the full nutritious fermentation liquor body fertilizer of the fresh earthworm of large-scale production
CN107712301A (en) * 2017-11-27 2018-02-23 无为县雨露生态农业有限公司 Improve the feed of meat chicken production performance
CN107751634A (en) * 2017-11-27 2018-03-06 无为县雨露生态农业有限公司 Yellow-feather broiler mixed feed
CN107751635A (en) * 2017-11-27 2018-03-06 无为县雨露生态农业有限公司 White meat-type chickens mixed feed

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106470692A (en) * 2014-03-29 2017-03-01 国立大学法人新泻大学 Suppress compositionss and its manufacture method of the fatty liver and renal hypertrophy being caused by diabetes
CN106470692B (en) * 2014-03-29 2019-12-03 国立大学法人新泻大学 Inhibit the composition and its manufacturing method of the fatty liver as caused by diabetes and hypernephrotrophy
CN106810399A (en) * 2017-02-16 2017-06-09 宁夏大学 The preparation method of the full nutritious fermentation liquor body fertilizer of the fresh earthworm of large-scale production
CN107712301A (en) * 2017-11-27 2018-02-23 无为县雨露生态农业有限公司 Improve the feed of meat chicken production performance
CN107751634A (en) * 2017-11-27 2018-03-06 无为县雨露生态农业有限公司 Yellow-feather broiler mixed feed
CN107751635A (en) * 2017-11-27 2018-03-06 无为县雨露生态农业有限公司 White meat-type chickens mixed feed

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