CN103005163A - Compound microorganism preparation for aquatic product - Google Patents

Compound microorganism preparation for aquatic product Download PDF

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Publication number
CN103005163A
CN103005163A CN2012105938090A CN201210593809A CN103005163A CN 103005163 A CN103005163 A CN 103005163A CN 2012105938090 A CN2012105938090 A CN 2012105938090A CN 201210593809 A CN201210593809 A CN 201210593809A CN 103005163 A CN103005163 A CN 103005163A
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CN
China
Prior art keywords
powder
complex microorganism
lactic acid
acid bacteria
bacillus subtilis
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CN2012105938090A
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Chinese (zh)
Inventor
王正丽
杨宁
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青岛农业大学
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Application filed by 青岛农业大学 filed Critical 青岛农业大学
Priority to CN2012105938090A priority Critical patent/CN103005163A/en
Publication of CN103005163A publication Critical patent/CN103005163A/en

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Abstract

The invention relates to a compound microorganism preparation for an aquatic product, comprising the following components of bacillus subtilis, bacillus licheniformis, lactobacillus, candida utilis and oligochitosan. The compound microorganism preparation prepared by the invention has the characteristics of no toxic and side effects, no pollution, no residues, low cost and the like, can inhibit the growth of pathogenic microorganisms, improves the self immunity of cultivated objects, maintains cultivation ecological balance and accelerates the growth of aquatic livestock.

Description

A kind of aquatic products complex microorganism preparations
Technical field
The invention belongs to field of feed additive technology, be specially the preparation and application that a kind of novel aquatic products is used complex microorganism preparations.
Background technology
In recent years, the culture fishery of China is fast-developing, and the intensive degree of cultivation improves constantly, and the water quality in breeding environment and cultivation waters worsens increasingly, is restricting the healthy and sustainable development of China's culture fishery, and is causing a series of environment and social concern.For this reason, relevant fishery authorities begin to promote the aquatic product health cultivation technology.So-called healthy aquaculture is exactly by throwing in without the epidemic disease seed, adopting reasonable aquaculture model and reinforcement to the management of input, the technical measures such as control breeding environment, make aquaculture organism keep the best g and D state, occur to reduce the farming disease harms, improve the quality of cultured product.
Using microorganism formulation is one of important technical of implementing aquatic product health cultivation, and it is under germ theory instructs, and utilizes in the cultivated animals body or the beneficial microbe of separating its living environment, the active bacteria formulation that adopts special process to make.It has have no side effect, pollution-free, noresidue and the characteristics such as low-cost, can suppress the growth of pathogenic microorganism, improve the immunity of cultivation object self, keep the breeding ecological balance.Therefore the invention provides a kind of novel aquatic products microorganism formulation, for aquaculture develops in a healthy way, realize that non-nuisance provides safeguard.
Summary of the invention
Purpose of the present invention provides a kind of aquatic products complex microorganism preparations, i.e. a kind of compound preparation that relates to several microorganisms, said preparation have remarkable quickening growth, improve aquatic livestock immunity, reduce the characteristics that disease occurs, thereby remedy the deficiencies in the prior art.
The present invention relates to a kind of aquatic products complex microorganism preparations, its component includes bacillus subtilis, lichens gemma bar, lactic acid bacteria, candida utili and chitosan oligomer.
Microorganism formulation of the present invention, one kind quality percentage is composed as follows: bacillus subtilis powder 20~30%, bacillus licheniformis powder 10~20%, freeze-dried lactic acid bacteria powder 10~20%, Candida utilis ferment powder 20~30%, chitosan oligomer 20~30%.
The preparation method of above-mentioned microorganism formulation is as follows: take by weighing at first respectively bacillus subtilis powder, bacillus licheniformis powder, freeze-dried lactic acid bacteria powder, candida utili powder and chitosan oligomer, sieve after grinding to form powdery, the screen cloth fineness is the 80-120 order, again powder is carried out drying and finishes preparation.
Complex microorganism preparations of the present invention is as the feed addictive of aquaculture.
The complex microorganism preparations that the present invention makes have have no side effect, pollution-free, noresidue and the characteristics such as low-cost, can suppress the growth of pathogenic microorganism, improve the immunity of cultivation object self, keep the breeding ecological balance, promote the growth of aquatic livestock.
The specific embodiment
Used bacillus subtilis and bacillus licheniformis are Gram-positive bacillus in the complex microorganism preparations of the present invention, are ubiquitous class aerobic bacterias.This bacterium can be present in the enteron aisle of aquatic animal and very strong protease, lipase, the amylase of secretion activity with endosporous form, but the utilization rate of Effective Raise feed promotes the aquatic animal growth; It also can be by eliminating or reducing pathogenic bacteria and improve water quality; The organic matters such as protein in water body and the bed mud, starch, fat can also be decomposed and absorb to bacillus to improve water quality and substrate.
Lactic acid bacteria is a kind of bacterium that can make carbohydrate fermentation produce lactic acid, and it can suppress the propagation of harmful microbe activity, pathogenic bacteria, organic corruption.Lactic acid bacteria can be decomposed at normal temperatures not labile lignin and cellulose, and organic matter fermentation is changed into the effective nutrient of animal and plant.
Candida utili is named again and produces protein torula or edible torula, and the content of its protein and Cobastab is all high than brewer's yeast, and it can be with urea and nitric acid as nitrogenous source, and not needing to add any growth factor in culture medium can grow.It can regulate the animal intestinal microbial balance simultaneously, improves feed digestibility, strengthens animal body immunity, promotes growing of animal, puts on weight.
Chitosan oligomer is the sugared little aggressiveness that is formed by connecting by glycosidic bond by 2 one a l0 monose.It can not be utilized by harmful bacteria hardly by the enzymic digestion of aquatic livestock self secretion, but can be by the beneficial bacterium utilization in the enteron aisle.Bifidobacterium in the intestines, Bacillus acidi lactici etc. all are the important beneficial bacterias in the alimentary canal, and chitosan oligomer carries out the stimulating growth breeding or activates metabolic function them, thereby plays the effect of regulating animal gastrointestinal micro-flora balance.
After being pulverized and mixed, above-mentioned four kinds of bacterium powder and a kind of chitosan oligomer make complex microorganism preparations of the present invention.
For a more clear understanding of the present invention and the curative effect of this complex microorganism preparations, the present invention is described in further detail by specific embodiment and correlation test below.
Embodiment 1
Take by weighing respectively by weight percentage available from Shandong Continent Biological Technology Co., Ltd bacillus subtilis powder 20g, bacillus licheniformis powder 15g, freeze-dried lactic acid bacteria powder 15g and Candida utilis ferment powder 20g and available from the chitosan oligomer 30g of the auspicious bio tech ltd of Jiaxing section, mixture is ground the rear sieve of using, and wherein the fineness requirement of sieve aperture is the 80-120 order.Thereby the pulvis after filtering is carried out drying make required microorganism formulation.
Embodiment 2
Take by weighing respectively by weight percentage bacillus subtilis powder 30g, bacillus licheniformis powder 10g available from North Sea group woods bioengineering Co., Ltd, freeze-dried lactic acid bacteria powder 10g, Candida utilis ferment powder 25g and believe to get the oligopolymerization chitosan Icing Sugar 25g of medicine company available from Shandong, mixture is ground the rear sieve of using, and wherein the fineness requirement of sieve aperture is the 80-120 order.Pulvis after filtering is carried out drying make required microorganism formulation.
The using method of mentioned microorganism preparation and consumption are: with the microorganism formulations 30min before use of embodiment 1 or 2 preparations, concentration with 0.1-0.3% is diluted 100-200 doubly with clear water, be sprayed onto on the feed, the aquatic livestock of throwing something and feeding after stirring guarantees that beneficial bacterium is not less than 10 in the content concentration of animal intestinal 7Individual/g.
The invention will be further described below in conjunction with index that the microorganism formulation feeding experiment draws.
Embodiment 3 actual cultivation are used
The test culturing pool is positioned at cultivation base, Qingdao of Shandong province Hong Dao district, two mouthfuls in test pond, and 1# is the contrast pool for the test pool, 2#, and area is 8 mu, and is smooth without ditch at the bottom of the pool, and water retention property is good, has abundant water resources.
High-quality Environment of Litopenaeus vannamei Low seedling (about specification 1.5cm) 70,000 tail/mus are thrown in two ponds.Throwing something and feeding in the test cooperates the shrimp material, and actual daily ration, feeding quantity is according to the adjustment such as the situation of ingesting of weather, water quality and shrimp and carry out daily management, and other any medicines are not all used at whole duration of test in two pools.
On June 15th, 2012 is microorganism formulation 30min before throwing something and feeding of embodiment 1 preparation, with clear water with the concentration dilution of 0.1-0.3% to 100-200 times, the Environment of Litopenaeus vannamei Low of throwing something and feeding after stirring 10 days, 2#(contrasts the pool) do not throw in the complex microorganism preparations preparation.Use per month once-combined microorganism formulation.Through the cultivation of four wheat harvesting periods, the rear discovery of cleaning up the pond of two pools, the duration of test complex microorganism preparations is to the regulating and controlling water quality successful, and use can obviously improve water quality continuously, effectively controls the content of nitrite nitrogen, ammonia nitrogen, can effectively keep the environment of control breeding water body.
1# actual output white shrimp 3577.5 kg, specification is 54 tails/kg, sells 128075 yuan of total prices; Do not use contrast pool actual output white shrimp 2560 kg of complex microorganism preparations, specification is 50 tails/kg, sell 92160 yuan of total prices, the actual volume increase of test group white shrimp 1017.5 kg, compare the control group disease and reduce, survival rate is high, fast growth, culture benefit increases by 35915 yuan, increases raiser's culture benefit.
Simultaneously, also the preparation effect with embodiment 1 is similar for the result of use of the microorganism formulation of embodiment 2 preparations, thereby has good cooperative effect between the component of proof microorganism formulation of the present invention, has good application value.

Claims (6)

1. aquatic products complex microorganism preparations, its component includes bacillus subtilis, bacillus licheniformis, lactic acid bacteria, candida utili and chitosan oligomer.
2. as claimed in claim complex microorganism preparations, its component is bacillus subtilis, bacillus licheniformis, lactic acid bacteria, candida utili and chitosan oligomer.
3. complex microorganism preparations as claimed in claim 1 or 2, its mass percent is composed as follows: bacillus subtilis powder 20~30%, bacillus licheniformis powder 10~20%, freeze-dried lactic acid bacteria powder 10~20%, Candida utilis ferment powder 20~30%, chitosan oligomer 20~30%.
4. the preparation method of claim 1 or 2 described complex microorganism preparations is as follows: take by weighing at first respectively bacillus subtilis powder, bacillus licheniformis powder, freeze-dried lactic acid bacteria powder, candida utili powder and chitosan oligomer, sieve after grinding to form powdery, the screen cloth fineness is the 80-120 order, again powder is carried out drying and finishes preparation.
5. claim 1 or the 2 described complex microorganism preparations application in the feed addictive of preparation aquaculture.
6. application as claimed in claim 5, be will preparation microorganism formulation 30min before use, with the concentration of 0.1-0.3% with clear water dilution 100-200 doubly, be sprayed onto on the feed aquatic livestock of throwing something and feeding after stirring.
CN2012105938090A 2012-12-29 2012-12-29 Compound microorganism preparation for aquatic product CN103005163A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103409352A (en) * 2013-08-19 2013-11-27 青岛农业大学 Compound microbial community for preparing feed by fermenting soybean hulls
CN103478445A (en) * 2013-08-22 2014-01-01 福建天马科技集团股份有限公司 Floating expanded pellet compound feed for adult fish in oncorhynchus mykiss
CN104431628A (en) * 2014-12-30 2015-03-25 张嘉强 Mixed feed for red tilapias
CN104543526A (en) * 2014-12-30 2015-04-29 湛江国联饲料有限公司 Trachinotus ovatus mixed feed
CN105174654A (en) * 2015-10-16 2015-12-23 中国科学院水生生物研究所 Deodorization method for landscape water body
CN107361250A (en) * 2017-08-22 2017-11-21 苏州神良生物科技有限公司 A kind of cultivation complex microorganism preparations
CN107361249A (en) * 2017-08-21 2017-11-21 苏州神良生物科技有限公司 A kind of freshwater aquiculture microorganism formulation
CN108815202A (en) * 2018-07-09 2018-11-16 江西省科学院生物资源研究所 A kind of chitosan microbial compound preparation and its preparation method and application

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103409352A (en) * 2013-08-19 2013-11-27 青岛农业大学 Compound microbial community for preparing feed by fermenting soybean hulls
CN103478445A (en) * 2013-08-22 2014-01-01 福建天马科技集团股份有限公司 Floating expanded pellet compound feed for adult fish in oncorhynchus mykiss
CN104431628A (en) * 2014-12-30 2015-03-25 张嘉强 Mixed feed for red tilapias
CN104543526A (en) * 2014-12-30 2015-04-29 湛江国联饲料有限公司 Trachinotus ovatus mixed feed
CN105174654A (en) * 2015-10-16 2015-12-23 中国科学院水生生物研究所 Deodorization method for landscape water body
CN105174654B (en) * 2015-10-16 2018-08-21 中国科学院水生生物研究所 A method of being used for landscape water body deodorization
CN107361249A (en) * 2017-08-21 2017-11-21 苏州神良生物科技有限公司 A kind of freshwater aquiculture microorganism formulation
CN107361250A (en) * 2017-08-22 2017-11-21 苏州神良生物科技有限公司 A kind of cultivation complex microorganism preparations
CN108815202A (en) * 2018-07-09 2018-11-16 江西省科学院生物资源研究所 A kind of chitosan microbial compound preparation and its preparation method and application
CN108815202B (en) * 2018-07-09 2019-07-30 江西省科学院生物资源研究所 A kind of chitosan microbial compound preparation and its preparation method and application

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