CN103621790A - Composite earthworm enzymatic hydrolysate feed additive and preparation method thereof - Google Patents
Composite earthworm enzymatic hydrolysate feed additive and preparation method thereof Download PDFInfo
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- CN103621790A CN103621790A CN201310619921.1A CN201310619921A CN103621790A CN 103621790 A CN103621790 A CN 103621790A CN 201310619921 A CN201310619921 A CN 201310619921A CN 103621790 A CN103621790 A CN 103621790A
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- earthworm
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- enzymolysis liquid
- cortex eucommiae
- folium cortex
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Abstract
The invention discloses a composite earthworm enzymatic hydrolysate feed additive and a preparation method thereof. The composite earthworm enzymatic hydrolysate feed additive is mainly prepared by mixing the following components in parts by weight: 50-70 parts of earthworm enzymatic hydrolysate, 30-50 parts of folium cortex eucommiae extracts, 3-8 parts of taurine, 1-3 parts of tyrosine and 0.1-0.3% sodium benzoate. The preparation method comprises the steps of (1) adding water and 1-3% papayotin into fresh earthworms according to the ratio of 1: (1-3), and carrying out enzymatic hydrolysis at the temperature of 40-70 DEG C for 30-60min while stirring; (2) after drying and smashing folium cortex eucommiae, adding water and carrying out ultrasonic extraction, concentration, drying and other processes to obtain the folium cortex eucommiae extracts; (3) sufficiently mixing the components to obtain the composite earthworm enzymatic hydrolysate feed additive. The feed additive is sufficiently mixed into a feed or bait and is fed after being aired. The composite earthworm enzymatic hydrolysate feed additive can be used for breeding various shrimps and particularly used for breeding penaeus vannamei, and is remarkable in breeding effect, simple in preparation process, capable of saving energy sources and time and easy to realize industrial production.
Description
Technical field
The present invention relates to a kind of feed addictive, especially utilize compound earthworm enzymolysis liquid feed addictive of earthworm enzymolysis liquid and eucommia leaf extract and preparation method thereof.
Background technology
The fresh earthworm of earthworm is high protein living bait.Livestock and poultry animal, length is eaten earthworm and can be reduced dosage, reduces the death rate.Freezing earthworm is high benefit medicinal bait, and various livestock and poultry animal length is eaten earthworm can improve meat, eggs and milk quality, reduces medicament residue.Fry field, aquatic farm, the long earthworm seedling of eating is strengthened, and body is strong, bright-colored.Earthworm has diuresis, eases pain, relievings asthma, step-down, the effect such as antipyretic, anticonvulsion.Earthworm contains the compositions such as earthworm element, lumbrofebrin, vitamin B complex complex.Earthworm can be extracted the biologics such as protease, Lumbrokinase, earthworm fibrinolysin.Earthworm can be used as the feed addictive of aquaculture through extracting the earthworm enzymolysis liquid preparing, increase the appetite of aquatic group, can be used for raising multiple shrimps, and especially for foster Penaeus Vannmei, culture efficiency is remarkable.Earthworm enzymolysis liquid
Folium cortex eucommiae not only has identical active ingredient and pharmacological action with Bark of Eucommia Ulmoides, and source is abundanter, and cost is lower.There is good exploitation prospect.Folium cortex eucommiae is the dry leaf of Eucommiaceae plant bark of eucommia Eucommia ulmoides Oliv..Summer, autumn gathered when two seasons, branches and leaves were luxuriant, dried or low temperature drying.Eucommia leaf extract composite feed additive can promote domestic animal, the health of aquatic products body, Fast Growth, and nontoxic pair is residual, helps domestic animal resist the disease.
Yet earthworm enzymolysis liquid, eucommia leaf extract are made to related compound feed addictive, there are no relevant report; And the problem that existing disclosed natural feed addictive exists is: 1, fail to make full use of the large agricultural product folium cortex eucommiae of Guangxi real estate leaf, wasted valuable crop resource; 2, fail to make full use of earthworm enzymolysis liquid, promotion aquaculture Prawn absorbs feed, and conventional additive main component is used the product of chemical element or grain processing.
Summary of the invention
The object of the invention is to the deficiency existing for natural feed addictive in prior art, a kind of composite feed additive that earthworm enzymolysis liquid, eucommia leaf extract be main material and preparation method thereof of take is provided, during use, this feed addictive is fully mixed in mixed feed or bait, dry or dry after throw something and feed.This product can be used for raising multiple shrimps, and especially for foster Penaeus Vannmei, culture efficiency is remarkable, and manufacturing process is simple, saves the energy and time, is easy to realize suitability for industrialized production.
To achieve these goals, the present invention has adopted following technical scheme:
A compound earthworm enzymolysis liquid feed addictive, this feed addictive is mainly mixed and is formed by the component of following parts by weight: earthworm enzymolysis liquid 50-70 part, 30~50 parts of eucommia leaf extracts, 3~8 parts of taurines, 1~3 part of TYR, 0.1-0.3% Sodium Benzoate.
As a further improvement on the present invention, described feed addictive is mainly mixed and is formed by the component of following parts by weight: 60 parts, 35 parts eucommia leaf extracts of earthworm enzymolysis liquid, 4 parts of taurines, 1 part of TYR, 0.15% Sodium Benzoate.
As a further improvement on the present invention, described earthworm enzymolysis liquid, uses fresh earthworm: water=1:1-3 adds water, then adds papain 1-3%, under 40-70 ℃ of condition, stirs enzymolysis 30-60 minute, the enzymolysis liquid of acquisition.
As a further improvement on the present invention, the eucommia leaf extract that after described eucommia leaf extract is folium cortex eucommiae drying and crushing, through adding water, ultrasonic wave extracts, obtain after concentrated, drying process.
A method of preparing above-described compound earthworm enzymolysis liquid feed addictive, is characterized in that: comprise the following steps:
(1) preparation of earthworm enzymolysis liquid:
Use fresh earthworm: water=1:1-3 adds water, then add papain 1-3%, under 40-70 ℃ of condition, stir enzymolysis 30-60 minute, the enzymolysis liquid of acquisition.
(2) preparation of eucommia leaf extract:
A. dry folium cortex eucommiae raw material is pulverized, then crossed 40-60 mesh sieve;
B. the folium cortex eucommiae raw material after pulverizing and sieving is put into tank, add the water of 5~10 times of folium cortex eucommiae raw material weights, be uniformly mixed and obtain folium cortex eucommiae slurries;
C. folium cortex eucommiae slurries are heated to 90~100 ℃, then use ultrasonic wave to extract after 80~120 minutes and filter, filtrate is concentrated into 40~50% rear rapid draings of virgin pulp liquid with film, obtain eucommia leaf extract.
(3) by following parts by weight, take each component: earthworm enzymolysis liquid 50-70 part, 30~50 parts of eucommia leaf extracts, 3~8 parts of taurines, 1~3 part of TYR, 0.1-0.3% Sodium Benzoate; each component is mixed; cross title, pack, obtain folium cortex eucommiae composite feed additive finished product.
The using method of the compound earthworm enzymolysis liquid feed addictive described in more than one, is characterized in that: by normal diet per ton, add the weight ratio of 2kg additive, by normal diet and additive mixing mixed feed or bait, dry or dry after throw something and feed.
Advantage of the present invention:
1. product can be used for raising multiple shrimps, and especially for foster Penaeus Vannmei, culture efficiency is remarkable.。
2. manufacturing process is simple, saves the energy and time, is easy to realize suitability for industrialized production.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described, but protection scope of the present invention is not limited to these embodiment.
Embodiment 1:
A compound earthworm enzymolysis liquid feed addictive, this feed addictive is mainly mixed and is formed by the component of following parts by weight: 55 parts, 45 parts eucommia leaf extracts of earthworm enzymolysis liquid, 7 parts of taurines, 3 parts of TYRs, 0.2% Sodium Benzoate.
The preparation method of this compound earthworm enzymolysis liquid feed addictive is as follows:
(1) preparation of earthworm enzymolysis liquid:
Use fresh earthworm: water=1:2 adds water, then add under 2.0%, 50 ℃ of condition of papain, stir enzymolysis 60 minutes, the enzymolysis liquid of acquisition.
(2) preparation of eucommia leaf extract:
A. dry folium cortex eucommiae raw material is pulverized, then crossed 50 mesh sieves;
B. the folium cortex eucommiae raw material after pulverizing and sieving is put into tank, add the water of 7 times of folium cortex eucommiae raw material weights, be uniformly mixed and obtain folium cortex eucommiae slurries;
C. folium cortex eucommiae slurries are heated to 90 ℃, then use ultrasonic wave to extract after 120 minutes and filter, filtrate is concentrated into 50% rear rapid draing of virgin pulp liquid with film, obtain eucommia leaf extract.
(3) by following parts by weight, take each component: 55 parts, 45 parts eucommia leaf extracts of earthworm enzymolysis liquid, 7 parts of taurines, 3 parts of TYRs, 0.2% Sodium Benzoate; each component is mixed; cross title, pack, obtain folium cortex eucommiae composite feed additive finished product.
Embodiment 2:
A compound earthworm enzymolysis liquid feed addictive, this feed addictive is mainly mixed and is formed by the component of following parts by weight: 65 parts, 40 parts eucommia leaf extracts of earthworm enzymolysis liquid, 2 parts of TYRs of 3 parts of taurines, 0.15% Sodium Benzoate.
The preparation method of this compound earthworm enzymolysis liquid feed addictive is as follows:
(1) preparation of earthworm enzymolysis liquid:
Use fresh earthworm: water=1:1 adds water, then add under 3%, 70 ℃ of condition of papain, stir enzymolysis 45 minutes, the enzymolysis liquid of acquisition.
(2) preparation of eucommia leaf extract:
A. dry folium cortex eucommiae raw material is pulverized, then crossed 60 mesh sieves;
B. the folium cortex eucommiae raw material after pulverizing and sieving is put into tank, add the water of 5 times of folium cortex eucommiae raw material weights, be uniformly mixed and obtain folium cortex eucommiae slurries;
C. folium cortex eucommiae slurries are heated to 100 ℃, then use ultrasonic wave to extract after 85 minutes and filter, filtrate is concentrated into 40% rear rapid draing of virgin pulp liquid with film, obtain eucommia leaf extract.
(3) by following parts by weight, take each component: 65 parts, 40 parts eucommia leaf extracts of earthworm enzymolysis liquid, 3 parts of taurines, 2 parts of TYRs, 0.15% Sodium Benzoate; each component is mixed; cross title, pack, obtain folium cortex eucommiae composite feed additive finished product.
Embodiment 3:
A compound earthworm enzymolysis liquid feed addictive, this feed addictive is mainly mixed and is formed by the component of following parts by weight: 60 parts, 30 parts eucommia leaf extracts of earthworm enzymolysis liquid, 7 parts of taurines, 3 parts of TYRs, 0.1% Sodium Benzoate.
The preparation method of this compound earthworm enzymolysis liquid feed addictive is as follows:
(1) preparation of earthworm enzymolysis liquid:
Use fresh earthworm: water=1:3 adds water, then add under 3%, 40 ℃ of condition of papain, stir enzymolysis 60 minutes, the enzymolysis liquid of acquisition.
(2) preparation of eucommia leaf extract:
A. dry folium cortex eucommiae raw material is pulverized, then crossed 40 mesh sieves;
B. the folium cortex eucommiae raw material after pulverizing and sieving is put into tank, add the water of 10 times of folium cortex eucommiae raw material weights, be uniformly mixed and obtain folium cortex eucommiae slurries;
C. folium cortex eucommiae slurries are heated to 100 ℃, then use ultrasonic wave to extract after 80 minutes and filter, filtrate is concentrated into 50% rear rapid draing of virgin pulp liquid with film, obtain eucommia leaf extract.
(3) by following parts by weight, take each component: 60 parts, 30 parts eucommia leaf extracts of earthworm enzymolysis liquid, 7 parts of taurines, 3 parts of TYRs, 0.1% Sodium Benzoate; each component is mixed; cross title, pack, obtain folium cortex eucommiae composite feed additive finished product.
Embodiment 4:
A compound earthworm enzymolysis liquid feed addictive, this feed addictive is mainly mixed and is formed by the component of following parts by weight: 60 parts, 35 parts eucommia leaf extracts of earthworm enzymolysis liquid, 4 parts of taurines, 1 part of TYR, 0.3% Sodium Benzoate.
The preparation method of this compound earthworm enzymolysis liquid feed addictive is as follows:
(1) preparation of earthworm enzymolysis liquid:
Use fresh earthworm: water=1:1.5 adds water, then add under 1.0%, 60 ℃ of condition of papain, stir enzymolysis 30 minutes, the enzymolysis liquid of acquisition.
(2) preparation of eucommia leaf extract:
A. dry folium cortex eucommiae raw material is pulverized, then crossed 50 mesh sieves;
B. the folium cortex eucommiae raw material after pulverizing and sieving is put into tank, add the water of 8 times of folium cortex eucommiae raw material weights, be uniformly mixed and obtain folium cortex eucommiae slurries;
C. folium cortex eucommiae slurries are heated to 100 ℃, then use ultrasonic wave to extract after 110 minutes and filter, filtrate is concentrated into 40% rear rapid draing of virgin pulp liquid with film, obtain eucommia leaf extract.
(3) by following parts by weight, take each component: 60 parts, 35 parts eucommia leaf extracts of earthworm enzymolysis liquid, 4 parts of taurines, 1 part of TYR, 0.3% Sodium Benzoate; each component is mixed; cross title, pack, obtain folium cortex eucommiae composite feed additive finished product.
Embodiment 5:
A compound earthworm enzymolysis liquid feed addictive, this feed addictive is mainly mixed and is formed by the component of following parts by weight: 70 parts, 30 parts eucommia leaf extract 0.3% Sodium Benzoates of earthworm enzymolysis liquid.
The preparation method of this compound earthworm enzymolysis liquid feed addictive is as follows:
(1) preparation of earthworm enzymolysis liquid:
Use fresh earthworm: water=1:3 adds water, then add under 1%, 70 ℃ of condition of papain, stir enzymolysis 60 minutes, the enzymolysis liquid of acquisition.
(2) preparation of eucommia leaf extract:
A. dry folium cortex eucommiae raw material is pulverized, then crossed 60 mesh sieves;
B. the folium cortex eucommiae raw material after pulverizing and sieving is put into tank, add the water of 9 times of folium cortex eucommiae raw material weights, be uniformly mixed and obtain folium cortex eucommiae slurries;
C. folium cortex eucommiae slurries are heated to 95 ℃, then use ultrasonic wave to extract after 100 minutes and filter, filtrate is concentrated into 45% rear rapid draing of virgin pulp liquid with film, obtain eucommia leaf extract.
(3) by following parts by weight, take each component: 70 parts, 30 parts eucommia leaf extracts of earthworm enzymolysis liquid, 0.3% Sodium Benzoate, each component is mixed, cross and claim, pack, obtain folium cortex eucommiae composite feed additive finished product.
Application example: Nanning City's in August, 2012 Yongning District aquatic farm
The weight ratio that adds 2kg additive by normal diet per ton, by normal diet and additive mixing mixed feed or bait, dry or dry after throw something and feed.
This aquatic farm is individual plant, has 2 mu of ponds for South America prawn culturing.This aquaculture factory South America shrimp speed of growth is slow before this, and the culture-cycle is 200 ~ 240 days, and South America shrimp from shrimp to cultivation, can to sell case fatality rate be 5 ~ 10%.It is object that 2 mu of South America shrimp aquacultures are take in experiment, is divided into two groups of experimental group and control groups (each mu, cultivation quantity is consistent), the high spot reviews speed of growth and case fatality rate.Experimental group use compound earthworm enzymolysis liquid feed addictive to dry by the using method spice in above-mentioned or dry after throw something and feed, feed to 100 days continuously, control group is according to daily management and forage feed.When experiment finishes, experimental group case fatality rate on average reduces by 30 ~ 40% than control group, and body weight increases by 10.2%, and effect is remarkable.
Claims (6)
1. a compound earthworm enzymolysis liquid feed addictive, is characterized in that: this feed addictive is mainly mixed and formed by the component of following parts by weight: earthworm enzymolysis liquid 50-70 part, 30~50 parts of eucommia leaf extracts, 3~8 parts of taurines, 1~3 part of TYR, 0.1-0.3% Sodium Benzoate.
2. compound earthworm enzymolysis liquid feed addictive according to claim 1, is characterized in that: described feed addictive is mainly mixed and formed by the component of following parts by weight: 60 parts, 35 parts eucommia leaf extracts of earthworm enzymolysis liquid, 4 parts of taurines, 1 part of TYR, 0.15% Sodium Benzoate.
3. compound earthworm enzymolysis liquid feed addictive according to claim 1 and 2, is characterized in that: described earthworm enzymolysis liquid, use fresh earthworm: water=1:1-3 adds water, add again papain 1-3%, under 40-70 ℃ of condition, stir enzymolysis 30-60 minute, the enzymolysis liquid of acquisition.
4. compound earthworm enzymolysis liquid feed addictive according to claim 3, is characterized in that: the eucommia leaf extract that after described eucommia leaf extract is folium cortex eucommiae drying and crushing, through adding water, ultrasonic wave extracts, obtain after concentrated, drying process.
5. a method of preparing the compound earthworm enzymolysis liquid feed addictive as described in as arbitrary in claim 1-4, is characterized in that: comprise the following steps:
(1) preparation of earthworm enzymolysis liquid:
Use fresh earthworm: water=1:1-3 adds water, then add papain 1-3%, under 40-70 ℃ of condition, stir enzymolysis 30-60 minute, the enzymolysis liquid of acquisition;
(2) preparation of eucommia leaf extract:
A. dry folium cortex eucommiae raw material is pulverized, then crossed 40-60 mesh sieve;
B. the folium cortex eucommiae raw material after pulverizing and sieving is put into tank, add the water of 5~10 times of folium cortex eucommiae raw material weights, be uniformly mixed and obtain folium cortex eucommiae slurries;
C. folium cortex eucommiae slurries are heated to 90~100 ℃, then use ultrasonic wave to extract after 80~120 minutes and filter, filtrate is concentrated into 40~50% rear rapid draings of virgin pulp liquid with film, obtain eucommia leaf extract;
(3) by following parts by weight, take each component: earthworm enzymolysis liquid 50-70 part, 30~50 parts of eucommia leaf extracts, 3~8 parts of taurines, 1~3 part of TYR, 0.1-0.3% Sodium Benzoate; each component is mixed; cross title, pack, obtain folium cortex eucommiae composite feed additive finished product.
6. the using method of the compound earthworm enzymolysis liquid feed addictive as described in as arbitrary in claim 1-4, it is characterized in that: the weight ratio that adds 2kg additive by normal diet per ton, by in normal diet and additive mixing mixed feed or bait, dry or dry after throw something and feed.
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Cited By (3)
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CN104886336A (en) * | 2015-06-15 | 2015-09-09 | 安徽金豪生态农业科技有限公司 | Extraction method of earthworm protein power |
CN107375341A (en) * | 2017-08-16 | 2017-11-24 | 北京艾克赛德生物工程有限公司 | A kind of preparation method of new earthworm powder |
CN110403186A (en) * | 2018-04-26 | 2019-11-05 | 王鹏 | A kind of earthworm zymolysis technique |
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Cited By (3)
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CN107375341A (en) * | 2017-08-16 | 2017-11-24 | 北京艾克赛德生物工程有限公司 | A kind of preparation method of new earthworm powder |
CN110403186A (en) * | 2018-04-26 | 2019-11-05 | 王鹏 | A kind of earthworm zymolysis technique |
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