CN103609909A - Pollution-free aquatic feed additive using ginkgo leaves as base stock - Google Patents

Pollution-free aquatic feed additive using ginkgo leaves as base stock Download PDF

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CN103609909A
CN103609909A CN201310637150.9A CN201310637150A CN103609909A CN 103609909 A CN103609909 A CN 103609909A CN 201310637150 A CN201310637150 A CN 201310637150A CN 103609909 A CN103609909 A CN 103609909A
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parts
mixture
take
ginkgo leaf
fermentation
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CN103609909B (en
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曾勇
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Guangdong hengwang Feed Co. Ltd.
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English Witter Jinjiang City Bioisystech Co Ltd
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Abstract

The invention relates to a pollution-free aquatic feed additive using ginkgo leaves as a base stock. The pollution-free aquatic feed additive is prepared from the following materials in parts by weight: 150-200 parts of ginkgo leaves, 100-120 parts of potatoes, 30-50 parts of lotus leaves, 15-20 parts of common andrographis herbs, 8-12 parts of Chinese mosla herbs, 5-7 parts of Chinese rhubarb, 7-12 parts of mangnolia officinalis, 3-5 parts of negundo chastetree fruits, 50-80 parts of chlorella powder, 50-80 parts of egg powder, 5-10 parts of medical stones and 20-30 parts of corn starch. The feed additive disclosed by the invention completely adopts natural components, is not extracted and treated by any harmful chemical reagent and pollution-free to an aquaculture environment, and completely satisfies green, safe and pollution-free requirements of modern aquaculture industry.

Description

A kind ofly take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material
Technical field
The invention belongs to field of fodder, relate to a kind of nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material of take.
Background technology
In recent years, along with expanding economy, people's living standard progressively improves, and entire society also progressively improves the demand of protein in food, development along with fishery and animal husbandry, the supply of meat can satisfy social needs substantially, particularly oppresses, as a kind of plain boiled pork, fat content is low, on market, be subject to liking of broad masses of the people, yet qualitatively, apart from people's expectation, also had certain distance.Degree in China's culture fishery scale is still not high, mainly with fishing ground and family's breed in stew of middle and small scale, is main, mainly adopts traditional method for breeding.Yet some problems of existence more or less in traditional breeding process, for example, in carp culture process, most raisers cultivate idea and fall behind, feed management does not catch up with, and a lot of raisers adopt low material, and its energy and albumen is wretched insufficiency often, cause carp growth speed excessively slow, feed conversion rate is low, and the cycle of feeding is long, grievous injury raiser's economic benefit; In grass carp breeding process, under high-density breeding condition, grass carp physique by feeding with fodder concentrate obviously declines, and cultivation density cross conference produce stress, reduce the resistance of fish to disease, cause survival rate low, the infringement of the gill rot being easily subject in breeding process, red skin disease, hemorrhage and enteritis disease, these four kinds of diseases are called as " grass carp four diseases ", and the outburst meeting of these diseases brings destructive strike to grass carp aquaculture, and the cultivation of grass carp makes to be subject to very large restriction; Intensive culture mode is also day by day serious to the infringement of water environment in addition, thereby causes aquatic products disease to take place frequently.Antibiotic use has certain effect to aquatic products disease prevention and cure tool within a certain period of time, but it is easy to expedite the emergence of out drug resistance strain, and Environmental Residues and the enrichment of fish body exist huge potential hazard to human health.Along with expanding economy, traditional aquatic products theory of raising no longer can meet the consumption demand that people are growing and improve, therefore, non-harmful feed addictive and non-harmful feed become the focus of feedstuff industry development in recent years, are also culturist and the consumer new requirements that development proposes to fishery cultivating.
Ginkgo is deciduous tree, is the most ancient Relict Plant in existing seed plant, and leaf is fan-shaped, scattered on long shoot, on brachyplast, clusters.Ginkgo is not only a kind of medicinal, economic fruit tree, in recent years along with the development of urban landscape construction and urban beautification, ginkgo has become a kind of ornamental plant, each avenue spreads all over the country, yet, except above effect, the whole body of ginkgo is all precious, wherein ginkgo leaf is except having the nutrition of general leaf, it also contains multiple flavonoids, terpene lactones, polyprenols and alkaloid and polysaccharide isoreactivity composition, can be used as the raw materials for production of medicine, biological pesticide, food, cosmetics.Also there is research used as feed addictive composition simultaneously, the mode of utilizing in feed industry mainly concentrates on direct utilization or simply after pulverizing, adds in feed at present, also there is part Study that its alcohol extract is used as to feed addictive, mainly play antioxidation, but while directly utilizing, its palatability is poor, biologically active is not obvious, cellulose, the large molecule such as hemicellulose is difficult to by animal use, adopt the mode of alcohol extracting to utilize, it has only utilized the wherein active component of part alcohol dissolubility, the mass efficient compositions such as cellulose and hemicellulose have been given up, utilization rate is not high yet.Therefore, can not effectively solve in the market the problem that ginkgo leaf utilizes, at present the also report for the production of feeding additive aquatic animal by ginkgo leaf or its extract not.
Summary of the invention
The problem of utilizing aspect to exist for ginkgo leaf in prior art, and culture fishery is for the demand of nuisance free feed supplement, the present invention aims to provide a kind of nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material of take.
For solving above technical problem, the present invention adopts following technological means:
The nuisanceless feeding additive aquatic animal that the ginkgo leaf of take is base-material, is characterized in that, by the raw material of following weight portion, is made: ginkgo leaf 150-200 part, potato 100-120 part, lotus leaf 30-50 part, Herba Andrographitis 15-20 part, elscholtiza 8-12 part, rheum officinale 5-7 part, bark of official magnolia 7-12 part, fruit of negundo 3-5 part, chlorella powder 50-80 part, egg powder 50-80 part, medical stone 5-10 part, cornstarch 20-30 part.
Described preparation method of take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material comprises the steps:
Step 1: take by weight each raw material;
Step 2: by the potato cutting taking in step 1, add the water of 5 times of its quality, heating is boiled and kept boiling 35m I n, is then cooled to 40 ℃; Simultaneously by the ginkgo leaf chopping taking in step 1, join cooling good potato and boil and boil in liquid, and add a kind of medical stone taking of step, obtain mixture I;
Step 3: the mixed bacteria that access is comprised of aspergillus niger and aspergillus oryzae in mixture I carries out aerobic fermentation, fermentation 3-5 days in the situation that stirring, then access again Lactobacillus plantarum, the in the situation that of sealing, carry out standing anaerobic fermentation, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, filtrate is carried out to vacuum freeze drying and obtain bacterium powder I, adopt the mode of heated-air drying to be dried filter residue, make its water content lower than 8%, then dry filter residue is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taking in step 1, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch are mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtaining in step 4 ,mixture IV in mixture III and step 5 is mixed, and mixes thoroughly and obtains the described nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material of take.
Preferably, by the raw material of following weight portion, made: 180 parts of ginkgo leaves, 110 parts of potatos, 40 parts, lotus leaf, 17 parts of Herba Andrographitis, 9 parts of elscholtizas, 6 parts of rheum officinales, 9 parts of the barks of official magnolia, 4 parts of fruits of negundo, 60 parts of chlorella powders, 60 parts of egg powders, 7 parts of medical stones, 25 parts of cornstarch.
Of the present inventionly take nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material and can be widely used in the raising of freshwater fish, preferably can be for the raising of grass carp, carp and black carp, its addition in feed is 1.0-2.0%.
Of the present inventionly take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material and have the following advantages and beneficial effect:
First, it is base-material that the present invention adopts urban waste-ginkgo leaf, mode by two-step fermentation is carried out fermentation process to ginkgo leaf, the wherein combination of aspergillus niger and aspergillus oryzae, can produce beta-glucosidase, by its bio-transformation, can make the flavour of a drug of ginkgo leaf and blue or green gas all weaken or disappear, and can make the glucoside type flavone compound of ginkgo leaf be converted into flavonols, strengthen biologically active, reached the effect of debitterize; Aspergillus niger and the aspergillus oryzae materials such as utilizing cellulose in ginkgo leaf and hemicellulose of can degrading simultaneously, be degraded to the utilizable small molecular sugar class of Lactobacillus plantarum, therefore after aspergillus niger and aspergillus oryzae fermentation, access again Lactobacillus plantarum fermentation, both can obtain the Lactobacillus plantarum bacterium liquid of higher concentration, simultaneously by above zymotechnique, can produce the fragrance of flowers and fruits and the mouthfeel of sweet acid, improve smell and the mouthfeel of ginkgo leaf.
Secondly, in preparation method of the present invention, do not add any harmful adding ingredient, in initial fermentation, select potato as carbon source, wherein be rich in the carbohydrates such as starch, contribute to aspergillus niger and the aspergillus oryzae Fast Growth at the fermentation initial stage, in fermentation system, add medical stone, trace element wherein and macroelement discharge slowly in the process of fermentation, can guarantee the nutrient demand in fermentation process; And both belong to natural food or traditional Chinese medicinal material raw materials, can not produce toxic action to animal.
Again, in nuisanceless feeding additive aquatic animal of the present invention, added multiple selected Chinese medicine composition, between itself and ginkgo leaf, cooperatively interact, to aquatic livestock grow and the raising of immunity has good facilitation, can also play the effect of diseases prevention simultaneously.Wherein being used in conjunction with of rheum officinale, the bark of official magnolia, Herba Andrographitis and fruit of negundo, can be good at playing antibacterial disease-resistant effect; The cooperation of egg powder and chlorella powder, makes feed have certain fishy smell, and fish are had to good food calling effect, is wherein rich in multiple proteins and vitamin simultaneously, can meet the nutritional need of fish, contributes to aquatic livestock to improve immunity.
Of the present inventionly take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material and adopt specific technique to process ginkgo leaf, again in conjunction with plurality of Chinese composition, can reach well the object that improves aquatic livestock immunity, promotes aquatic animal to grow, and feed addictive of the present invention all adopts natural component, without any harmful chemical reagent extraction process, pollution-free to culture environment of aquatic products, meet modern culture fishery green, safety, non-harmful requirement completely.
Specific embodiment
embodiment 1: a kind ofly take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material, by the raw material of following weight portion, made: 180 parts of ginkgo leaves, 110 parts of potatos, 40 parts, lotus leaf, 17 parts of Herba Andrographitis, 9 parts of elscholtizas, 6 parts of rheum officinales, 9 parts of the barks of official magnolia, 4 parts of fruits of negundo, 60 parts of chlorella powders, 60 parts of egg powders, 7 parts of medical stones, 25 parts of cornstarch.
Described preparation method of take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material comprises the steps:
Step 1: take by weight each raw material;
Step 2: by the potato cutting taking in step 1, add the water of 5 times of its quality, heating is boiled and kept boiling 35m I n, is then cooled to 40 ℃; Simultaneously by the ginkgo leaf chopping taking in step 1, join cooling good potato and boil and boil in liquid, and add a kind of medical stone taking of step, obtain mixture I;
Step 3: the mixed bacteria that access is comprised of aspergillus niger and aspergillus oryzae in mixture I carries out aerobic fermentation, fermentation 3-5 days in the situation that stirring, then access again Lactobacillus plantarum, the in the situation that of sealing, carry out standing anaerobic fermentation, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, filtrate is carried out to vacuum freeze drying and obtain bacterium powder I, adopt the mode of heated-air drying to be dried filter residue, make its water content lower than 8%, then dry filter residue is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taking in step 1, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch are mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtaining in step 4 ,mixture IV in mixture III and step 5 is mixed, and mixes thoroughly and obtains the described nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material of take.
embodiment 2: a kind ofly take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material, by the raw material of following weight portion, made: 150 parts of ginkgo leaves, 120 parts of potatos, 35 parts, lotus leaf, 20 parts of Herba Andrographitis, 10 parts of elscholtizas, 6 parts of rheum officinales, 10 parts of the barks of official magnolia, 5 parts of fruits of negundo, 80 parts of chlorella powders, 50 parts of egg powders, 8 parts of medical stones, 25 parts of cornstarch.
Described preparation method of take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material comprises the steps:
Step 1: take by weight each raw material;
Step 2: by the potato cutting taking in step 1, add the water of 5 times of its quality, heating is boiled and kept boiling 35m I n, is then cooled to 40 ℃; Simultaneously by the ginkgo leaf chopping taking in step 1, join cooling good potato and boil and boil in liquid, and add a kind of medical stone taking of step, obtain mixture I;
Step 3: the mixed bacteria that access is comprised of aspergillus niger and aspergillus oryzae in mixture I carries out aerobic fermentation, fermentation 3-5 days in the situation that stirring, then access again Lactobacillus plantarum, the in the situation that of sealing, carry out standing anaerobic fermentation, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, filtrate is carried out to vacuum freeze drying and obtain bacterium powder I, adopt the mode of heated-air drying to be dried filter residue, make its water content lower than 8%, then dry filter residue is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taking in step 1, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch are mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtaining in step 4 ,mixture IV in mixture III and step 5 is mixed, and mixes thoroughly and obtains the described nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material of take.
embodiment 3: safety testing
Acute toxicity test in mice: the feed addictive of the embodiment of the present invention 1 mix 15%(weight percentage in the feed of small white mouse), the feeding for small white mouse by the feed after this mix, Continuous Observation 20 days, duration of test has no dead and undue toxicity reaction, off-test, Dissection test mouse, observes liver,kidney,spleen, stomach, the heart, lung and intestines, is showed no abnormal change.Press acute toxicity standard, this feed addictive belongs to nontoxic level.
Chronic toxicity test: the feed addictive of the embodiment of the present invention 1 mix 7%(weight percentage in the feed of small white mouse), the feeding for small white mouse by the feed after this mix, feed continuously 120 days, result shows: the mouse fur of the feed that comprises the embodiment of the present invention 1 feed addictive of feeding is smooth, behavioral activity is normal, the drinking-water of ingesting is normal, Normal-weight increases, blood parameters and hematology all fluctuate in normal range (NR), routine urinalysis and marrow index all fluctuate in normal range (NR), organize internal organs no abnormality seen, generally speaking have no toxicity reaction.
From testing above, product of the present invention is safety, nontoxic.
embodiment 4: Animal experiment
Comparative experimental example 1 and 2 are below set:
comparative experimental example 1: a kind of feed addictive, by the raw material of following weight portion, made: 180 parts of ginkgo leaves, 110 parts of potatos, 40 parts, lotus leaf, 9 parts of the barks of official magnolia, 4 parts of fruits of negundo, 60 parts of chlorella powders, 60 parts of egg powders, 7 parts of medical stones, 25 parts of cornstarch.The preparation method of described feed addictive comprises the steps:
Step 1: take by weight each raw material;
Step 2: by the potato cutting taking in step 1, add the water of 5 times of its quality, heating is boiled and kept boiling 35m I n, is then cooled to 40 ℃; Simultaneously by the ginkgo leaf chopping taking in step 1, join cooling good potato and boil and boil in liquid, and add a kind of medical stone taking of step, obtain mixture I;
Step 3: the mixed bacteria that access is comprised of aspergillus niger and aspergillus oryzae in mixture I carries out aerobic fermentation, fermentation 3-5 days in the situation that stirring, then access again Lactobacillus plantarum, the in the situation that of sealing, carry out standing anaerobic fermentation, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, filtrate is carried out to vacuum freeze drying and obtain bacterium powder I, adopt the mode of heated-air drying to be dried filter residue, make its water content lower than 8%, then dry filter residue is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taking in step 1, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch are mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtaining in step 4 ,mixture IV in mixture III and step 5 is mixed, and mixes the feed addictive that obtains comparative experimental example 1 thoroughly.
comparative experimental example 2: a kind of feed addictive, by the raw material of following weight portion, made: 180 parts of ginkgo leaves, 110 parts of potatos, 40 parts, lotus leaf, 17 parts of Herba Andrographitis, 9 parts of elscholtizas, 9 parts of the barks of official magnolia, 4 parts of fruits of negundo, 7 parts of medical stones, 25 parts of cornstarch.The preparation method of described feed addictive comprises the steps:
Step 1: take by weight each raw material;
Step 2: by the potato cutting taking in step 1, add the water of 5 times of its quality, heating is boiled and kept boiling 35m I n, is then cooled to 40 ℃; Simultaneously by the ginkgo leaf chopping taking in step 1, join cooling good potato and boil and boil in liquid, and add a kind of medical stone taking of step, obtain mixture I;
Step 3: the mixed bacteria that access is comprised of aspergillus niger and aspergillus oryzae in mixture I, in the situation that stirring, carry out aerobic fermentation, fermentation 3-5 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, filtrate is carried out to vacuum freeze drying and obtain bacterium powder I, adopt the mode of heated-air drying to be dried filter residue, make its water content lower than 8%, then dry filter residue is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taking in step 1, Herba Andrographitis, elscholtiza, the bark of official magnolia, fruit of negundo and cornstarch are mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtaining in step 4 ,mixture IV in mixture III and step 5 is mixed, and mixes the feed addictive that obtains comparative experimental example 2 thoroughly.
(1) grass carp feeding experiment
Get 5 of same a slice waters, same batch of grass carp aquaculture net cages of putting in a suitable place to breed, in every net cage, cultivation grass carp 200 tails, are divided into 5 groups at random, organize 1 for blank group, the grass carp pellet of throwing something and feeding commercially available; Group 2-3 is test group, and its feed of throwing something and feeding is on the basis of commercially available grass carp pellet formula, to have added the feeding additive aquatic animal of 1.5% the embodiment of the present invention 1,2 the grass carp pellet still processing according to conventional method; Group 4-5 is check experiment group, and its feed of throwing something and feeding is on the basis of commercially available grass carp pellet formula, to have added the feed addictive of 1.5% comparative experimental example 1,2 the grass carp pellet still processing according to conventional method.Feed and within 60 days, gather in the crops afterwards (being whole test duration 60 days), before feeding, each group is fished for 5 tails at random, take its initial weight and on tail fin, stay corresponding label, feeding experiment finishes the rear body weight that continues to take fish survival, mark, the average weight growth pattern of grass carp is respectively organized in representative, the death rate of grass carp in the process of feeding respectively organized in record simultaneously, and concrete outcome is as follows:
Group 1: blank group, average growth rate 219.3%, during dead grass carp 15 tails of this net cage, the death rate is 7.5%, there is not death in mark grass carp.The individual integral body of this group grass carp is less.
Group 2: 1 group of embodiment, average growth rate 327.9%, during this net cage dead for there is grass carp, the death rate is 0%.This group grass carp individuality is maximum in 5 groups, and counterpoise has reached 4.76kg.
Group 3: 2 groups of embodiment, average growth rate 312.2%, during dead grass carp 1 tail of this net cage, the death rate is 0.5%, there is not death in mark grass carp.This group grass carp individuality is inferior large in 5 groups, and counterpoise has reached 4.57kg.
Group 4: 1 group of comparative example, average growth rate 261.3%, during dead grass carp 5 tails of this net cage, the death rate is 2.5%, there is not death in mark grass carp.This group grass carp is individual little.
Group 5: 2 groups of comparative examples, average growth rate 258.1%, during dead grass carp 9 tails of this net cage, the death rate is 4.5%, there is not death in mark grass carp.This group grass carp individuality is less than normal.
Average growth rate=(the initial TBW of test TBW-mark in latter stage fish of mark fish)/initial TBW * 100% wherein.
From above result of the test, product of the present invention can promote the growth of grass carp effectively, its average growth rate is apparently higher than blank group and group 4(comparative experimental example 1), group 5(comparative experimental example 2), no matter illustrate it is that formula forms or preparation technology, be absolutely necessary for reaching all of technique effect.Adopt product of the present invention to throw something and feed, the death rate of grass carp is organized 1, is organized also decline to some extent of 4-5, proves that feed addictive of the present invention can improve the immunity of grass carp effectively, improves its resistance against diseases.
(2) carp feeding experiment
Get 5, same a slice waters, same batch of carp culture net cage of putting in a suitable place to breed, in every net cage, cultivation carp 100 tails, are divided into 5 groups at random, organize 1 for blank group, the carp pellet of throwing something and feeding commercially available; Group 2-3 is test group, and its feed of throwing something and feeding is on the basis of commercially available carp pellet formula, to have added the feeding additive aquatic animal of 2.0% the embodiment of the present invention 1,2 the carp pellet still processing according to conventional method; Group 4-5 is check experiment group, and its feed of throwing something and feeding is on the basis of commercially available carp pellet formula, to have added the feed addictive of 2.0% comparative experimental example 1,2 the carp pellet still processing according to conventional method.Feed and within 80 days, gather in the crops afterwards (being whole test duration 80 days), before feeding, each group is fished for 5 tails at random, take its initial weight and on tail fin, stay corresponding label, feeding experiment finishes the rear body weight that continues to take fish survival, mark, the average weight growth pattern of carp is respectively organized in representative, the death rate of carp in the process of feeding respectively organized in record simultaneously, and concrete outcome is as follows:
Group 1: blank group, average growth rate 199.4%, during dead carp 8 tails of this net cage, the death rate is 8.0%, there is not death in mark carp.This group carp individual integral body is less.
Group 2: 1 group of embodiment, average growth rate 279.3%, during dead carp 0 tail of this net cage, the death rate is 0%.This group carp individual is maximum in 5 groups, and counterpoise has reached 3.27kg.
Group 3: 2 groups of embodiment, average growth rate 267.4%, during dead carp 2 tails of this net cage, the death rate is 2.0%, there is not death in mark carp.This group carp individual is inferior large in 5 groups, and counterpoise has reached 3.07kg.
Group 4: 1 group of comparative example, average growth rate 212.2%, during dead carp 4 tails of this net cage, the death rate is 4.0%, there is not death in mark carp.This group carp individual is little.
Group 5: 2 groups of comparative examples, average growth rate 218.7%, during dead carp 9 tails of this net cage, the death rate is 9.0%, there is not death in mark carp.This group carp individual is less than normal.
Average growth rate=(the initial TBW of test TBW-mark in latter stage fish of mark fish)/initial TBW * 100% wherein.
From above result of the test, product of the present invention can promote the growth of carp effectively, its average growth rate is apparently higher than blank group and group 4(comparative experimental example 1), group 5(comparative experimental example 2), no matter illustrate it is that formula forms or preparation technology, be absolutely necessary for reaching all of technique effect.Adopt product of the present invention to throw something and feed, the death rate of carp is organized 1, is organized also decline to some extent of 4-5, proves that feed addictive of the present invention can improve the immunity of carp effectively, improves its resistance against diseases.
In the extension process of product of the present invention, find that its growth to Freshwater shrimps, crucian, black carp has good facilitation.
Raw material of the present invention all can be bought from the market.Disclosed is above only specific embodiments of the invention, but the present invention is not limited thereto, and the changes that any person skilled in the art can think of, within all should dropping on protection scope of the present invention.

Claims (4)

1. the nuisanceless feeding additive aquatic animal that the ginkgo leaf of take is base-material, is characterized in that, by the raw material of following weight portion, is made: ginkgo leaf 150-200 part, potato 100-120 part, lotus leaf 30-50 part, Herba Andrographitis 15-20 part, elscholtiza 8-12 part, rheum officinale 5-7 part, bark of official magnolia 7-12 part, fruit of negundo 3-5 part, chlorella powder 50-80 part, egg powder 50-80 part, medical stone 5-10 part, cornstarch 20-30 part.
2. according to claim 1ly take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material, it is characterized in that, its preparation method comprises the steps:
Step 1: take by weight each raw material;
Step 2: by the potato cutting taking in step 1, add the water of 5 times of its quality, heating is boiled and kept boiling 35m I n, is then cooled to 40 ℃; Simultaneously by the ginkgo leaf chopping taking in step 1, join cooling good potato and boil and boil in liquid, and add a kind of medical stone taking of step, obtain mixture I;
Step 3: the mixed bacteria that access is comprised of aspergillus niger and aspergillus oryzae in mixture I carries out aerobic fermentation, fermentation 3-5 days in the situation that stirring, then access again Lactobacillus plantarum, the in the situation that of sealing, carry out standing anaerobic fermentation, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, filtrate is carried out to vacuum freeze drying and obtain bacterium powder I, adopt the mode of heated-air drying to be dried filter residue, make its water content lower than 8%, then dry filter residue is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taking in step 1, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch are mixed, obtain mixture IV;
Step 6: by the bacterium powder obtaining in step 4 i,mixture IV in mixture III and step 5 is mixed, and mixes thoroughly and obtains the described nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material of take.
3. according to claim 1 and 2ly take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material, it is characterized in that, by the raw material of following weight portion, made: 180 parts of ginkgo leaves, 110 parts of potatos, 40 parts, lotus leaf, 17 parts of Herba Andrographitis, 9 parts of elscholtizas, 6 parts of rheum officinales, 9 parts of the barks of official magnolia, 4 parts of fruits of negundo, 60 parts of chlorella powders, 60 parts of egg powders, 7 parts of medical stones, 25 parts of cornstarch.
4. prepare the method for take the nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material described in claim 1-2 any one, it is characterized in that, comprise the steps: step 1: take by weight each raw material;
Step 2: by the potato cutting taking in step 1, add the water of 5 times of its quality, heating is boiled and kept boiling 35m I n, is then cooled to 40 ℃; Simultaneously by the ginkgo leaf chopping taking in step 1, join cooling good potato and boil and boil in liquid, and add a kind of medical stone taking of step, obtain mixture I;
Step 3: the mixed bacteria that access is comprised of aspergillus niger and aspergillus oryzae in mixture I carries out aerobic fermentation, fermentation 3-5 days in the situation that stirring, then access again Lactobacillus plantarum, the in the situation that of sealing, carry out standing anaerobic fermentation, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, filtrate is carried out to vacuum freeze drying and obtain bacterium powder I, adopt the mode of heated-air drying to be dried filter residue, make its water content lower than 8%, then dry filter residue is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taking in step 1, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch are mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtaining in step 4 ,mixture IV in mixture III and step 5 is mixed, and mixes thoroughly and obtains the described nuisanceless feeding additive aquatic animal that ginkgo leaf is base-material of take.
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CN105412349A (en) * 2015-11-16 2016-03-23 郎溪县飞鲤镇幸福好运家畜养殖家庭农场 Natural fish medicine capable of resisting bacteria and preventing diseases
CN106417127A (en) * 2016-10-13 2017-02-22 合肥润雨农业科技有限公司 Handling method for lowering content of diethylstilbestrol in prawns
CN107259087A (en) * 2017-07-10 2017-10-20 张松波 A kind of disease-resistant Chinese herbal feed additive and preparation method thereof

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CN101708030A (en) * 2008-09-07 2010-05-19 王妍 Ginkgo biloba leaf feed
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CN101543259A (en) * 2009-05-08 2009-09-30 南京林业大学 Preparation method of biological feed additive
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CN105412349A (en) * 2015-11-16 2016-03-23 郎溪县飞鲤镇幸福好运家畜养殖家庭农场 Natural fish medicine capable of resisting bacteria and preventing diseases
CN106417127A (en) * 2016-10-13 2017-02-22 合肥润雨农业科技有限公司 Handling method for lowering content of diethylstilbestrol in prawns
CN107259087A (en) * 2017-07-10 2017-10-20 张松波 A kind of disease-resistant Chinese herbal feed additive and preparation method thereof

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