CN104095154B - A kind of Cynoglossus semilaevis special immune reinforcing agent - Google Patents
A kind of Cynoglossus semilaevis special immune reinforcing agent Download PDFInfo
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- CN104095154B CN104095154B CN201410164471.6A CN201410164471A CN104095154B CN 104095154 B CN104095154 B CN 104095154B CN 201410164471 A CN201410164471 A CN 201410164471A CN 104095154 B CN104095154 B CN 104095154B
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- reinforcing agent
- cynoglossus semilaevis
- special immune
- immune reinforcing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention belongs to technical field of aquaculture, particularly a kind of Cynoglossus semilaevis special immune reinforcing agent.A kind of Cynoglossus semilaevis special immune reinforcing agent, its component primarily of following weight portion mixes: beta glucan 0.2-0.4 part, Radix Acanthopanacis Senticosi root 4-12 part, ampelopsis grossdentata 5-10 part, agate card 10-15 part, Kluyveromyces fragilis 12-15 part, folium cortex eucommiae 10-20 part, Fructus Hordei Germinatus 5-10 part, bamboo vinegar 65-80 part.In aquaculture industry, abuse of antibiotics can not be ignored, Cynoglossus semilaevis special immune reinforcing agent of the present invention adopts pure natural plant ingredients, auxiliary a small amount of beta glucan, effectively can strengthen the immunoenhancement result of composition, and this component is not containing antibiotic, avoids a series of consequences that abuse of antibiotics causes.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly a kind of Cynoglossus semilaevis special immune reinforcing agent.
Background technology
Cynoglossus semilaevis (CynoglossussemilaevisGunther) belongs to Pleuronectiformes, Cynoglossidae, tongue sole genus, is commonly called as " imperial Lei ", field sowthistle herb, sole order, sole Ban, Ruo sole, thin squama, sole rice.Be the large-scale demersal fishes in a kind of warm warm nature coastal waters, life is all the year round perched in CHINESE OFFSHORE sea area, the wide temperature of tool, wide salt and adapt to the feature of changeable environmental condition, suitable temperature range 3.5-32 DEG C, optimum water temperature 14-24 DEG C, proper salinity level 14-33 ‰.Cynoglossus semilaevis natural resources amount is few, delicious deliciousness, and dressing percentage is high, smooth in taste, and the flesh of fish boils for a long time and inalways, without fishy smell and peculiar smell, belongs to high protein, nutritious.In recent years, the breeding and cultivate and rise rapidly in China's Coastal Areas of Cynoglossus semilaevis.Cynoglossus semilaevis both can adopt the industrial aquaculture of high investment, also can carry out the pond culture of low cost.Cynoglossus semilaevis is a kind newly developed, and is sole class first man work breed variety.Along with the development propagating industry artificially of Cynoglossus semilaevis, its disease and pest also increases gradually, often some disease and pests can occur, and causes the immunity degradation of Cynoglossus semilaevis, occurs the phenomenon that Cynoglossus semilaevis immunity degradation is even dead.Therefore, how to improve the immunity of Cynoglossus semilaevis body, the invasion of effective resist the disease, reduce morbidity and the death rate, become the study hotspot of current cynoglossus semilaevis cultivation.
Immunopotentiator is also known as immunopotentiating agent, refer to the material using with antigen individually or simultaneously and all can strengthen animal immune and react, enhancing fish resist the ability that extraneous pathogen (bacterium, fungi, virus and parasite etc.) is attacked, and also can alleviate the immunosuppressive action of external environment Stress on Fish simultaneously.The complicated variety of immunopotentiator kind and the mechanism of action brings an a lot of difficult problem to its use in actual production.Because immunopotentiator needs to purify, put forward effect or pulverize use, and endless unrest can not throw something and feed, therefore need to consider the series of problems such as processing technology, dosage regimen and compatibility.The immunopotentiator that different fish are suitable for is not identical.At present, the immunopotentiator being applicable to cynoglossus semilaevis cultivation is not yet had.
Summary of the invention
For problems of the prior art, the invention provides a kind of Cynoglossus semilaevis special immune reinforcing agent, this immunopotentiator effectively can improve immunity and the premunition of Cynoglossus semilaevis, and initiatively protection effect is better.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of Cynoglossus semilaevis special immune reinforcing agent, its component primarily of following weight portion mixes: beta glucan 0.2-0.4 part, Radix Acanthopanacis Senticosi root 4-12 part, ampelopsis grossdentata 5-10 part, agate card 10-15 part, Kluyveromyces fragilis 12-15 part, folium cortex eucommiae 10-20 part, Fructus Hordei Germinatus 5-10 part, bamboo vinegar 65-80 part.In aquaculture industry, abuse of antibiotics can not be ignored, Cynoglossus semilaevis special immune reinforcing agent of the present invention adopts pure natural plant ingredients, auxiliary a small amount of beta glucan, effectively can strengthen the immunoenhancement result of composition, and this component is not containing antibiotic, avoids a series of consequences that abuse of antibiotics causes.
As preferably, described bamboo vinegar is that the vaporization liquid that decomposes of bamboo wood high-temperature heating is through cooled product.Inventor's lot of experiment validation, bamboo vinegar consumption too much can cause immunopotentiator overall acid excessive, is unfavorable for the growth of Cynoglossus semilaevis, and bamboo vinegar consumption is crossed that I haven't seen you for ages and cause each component of epidemic disease reinforcing agent to mix rear formability bad, and skeptophylaxis miopragia.
As preferably, described Cynoglossus semilaevis special immune reinforcing agent mixes primarily of the component of following weight portion: beta glucan 0.2-0.4 part, Radix Acanthopanacis Senticosi root 4-6 part, ampelopsis grossdentata 8-10 part, agate card 10-12 part, Kluyveromyces fragilis 12-15 part, folium cortex eucommiae 18-20 part, Fructus Hordei Germinatus 5-8 part, bamboo vinegar 65-70 part.
A preparation method for described Cynoglossus semilaevis special immune reinforcing agent, is characterized in that the method comprises the steps: a, by through the Radix Acanthopanacis Senticosi root of super-dry process, ampelopsis grossdentata, Ma Ka and folium cortex eucommiae mixing, crosses 150-200 mesh sieve after pulverizing; B, Fructus Hordei Germinatus mix with bamboo vinegar soak 10-12 hour after pulverize slurrying; The slurry that c, the powder obtained by step a and step b obtain mixes and adds beta glucan and Kluyveromyces fragilis, fully obtains product after mixing.
As preferably, described ampelopsis grossdentata is the young young stem and leaf of ampelopsis grossdentata plant.
Described Cynoglossus semilaevis special immune reinforcing agent is preparing the application in feed, and the described addition of Cynoglossus semilaevis special immune reinforcing agent in feed is 0.2-4wt%.
Beta glucan derives from fresh food brewer's yeast, and it is a kind of polysaccharide, general chemical structure β-1,3 glucan and β-1,6 glucan, and wherein the former has antitumor character, and can greatly improve human body natural's immunity.Inventor, through studying discovery for a long time, is used alone beta glucan and carries out assisting in feeding to Cynoglossus semilaevis, and immunity strengthens DeGrain.
Wilsonii is a kind of conventional Chinese medicine, contains through researching and analysing Main Ingredients and Appearance: the carrotene of multiple carbohydrate, amino acid, aliphatic acid, vitamin A, B1, B2 and volume; Another containing sesamin, sterol, coumarin, flavones, suberone, nonaro-maticity unsaturated organic acid and lot of trace mineral matter etc.Wilsonii is recorded in " practical tonic Chinese medicine one book ", belongs to Qi-tonifying drug, has effect that qi-restoratives is helped the weak, can prevent or treat the disease had a delicate constitution, strengthening by means of tonics, promoting longevity.The root getting wilsonii in the present invention uses.
Ampelopsis grossdentata (formal name used at school: Ampelopsisgrossedentata) is the plant of Vitaceae Ampelopsis, is the endemic plant of China.In recent years, more to ampelopsis grossdentata research, effects such as finding that there is anti-inflammatory, cough-relieving, analgesia, lipopenicillinase, hypoglycemic, anti-hypertension, protect the liver, be antibacterial, anti-oxidant.The present invention makes powder and other component compatibilities after adopting its young young stem and leaf to dry.
Maca powder is the dry powder of plant Maca, the chemical composition composition in the dry root of agate card: protein content more than 10%, the carbohydrate of 59%; The fiber of 8.5%, include the mineral matters such as abundant zinc, calcium, iron, titanium, rubidium, potassium, sodium, copper, manganese, magnesium, strontium, phosphorus, iodine, and containing vitamin C, B1, B2, B6, A, E, B12, B5, fat content is not high but mostly wherein be unrighted acid, the content of linoleic acid plus linolenic acid reaches more than 53%, and natural activity composition comprises alkaloid, glucosinolate and catabolite benzyl isothiocyanate, sterol, polyphenols etc.Maca has various biological active material, can use as physical reinforcing agent.
Kluyveromyces fragilis, be through cultivating, drying is made does not have fermenting power, cell is the Powdered of dead state or granular product.Kluyveromyces fragilis contains the materials such as rich in protein (about 30 ~ 40%), B family vitamin, amino acid, is widely used as the protein supplements of animal feed.It can promote growing of animal, shortens feeding period, increases meat amount and egg amount, and improving pork quality and raising lean meat percentage, improve the glossiness of fur, and can strengthen the resistance against diseases of poult poultry.
Containing bark of eucommia chlorogenic acid in folium cortex eucommiae, there is antibacterial action more widely.
The compositions such as Fructus Hordei Germinatus amylase-containing, conversion carbohydrase, protein, protease, Cobastab, lecithin, maltose, glucose.Fructus Hordei Germinatus mix with bamboo vinegar soak after the composition of Fructus Hordei Germinatus is more easily absorbed, have nourishing benefiting action.Fructus Hordei Germinatus also uses as excipient in this component, makes the immunopotentiator formability made good.
Described bamboo vinegar is that the vaporization liquid of bamboo wood high-temperature heating decomposition is through cooled product, bamboo vinegar is in the process of making charcoal with bamboo wood, collect the gas that bamboo wood produces in pyrolytic, and this gas being cooled at normal temperatures the liquid substance obtained, the collection of bamboo vinegar should be carry out between 80 DEG C ~ 150 DEG C in the throat temperature of charcoal kiln.The bamboo vinegar of thick extraction is coffee-like, and have the smell of burning and tart flavour, in composition, 80%-90% is water, and acetic acid accounts for 3%, tar accounts for 1%, and other still have multiple organic principle, comprises carboxylic acids 6 kinds, fragrant lopps 19 kinds, aldehydes 2 kinds, ketone 16 kinds, ester class 7 kinds, alcohol and ethers 4 kinds etc.After the bamboo vinegar slightly extracted being left standstill 3-6 month, be affected by gravity, bamboo vinegar is divided into three layers, and the heaviest tar is at the bottom, and centre is bamboo vinegar water, and upper strata is tar light oil.Get after middle bamboo vinegar water carries out several distillation, color from light yellow changes transparent bamboo vinegar into, and this bamboo vinegar separately as immunopotentiator Use Limitation really and not obvious.
Research display, each raw material in immunopotentiator of the present invention, when feeding separately, all cannot improve immunity and the premunition of Cynoglossus semilaevis, but individual components is effective not too obvious.And each constituent content of the present invention is through the mixing of suitable ratio, active ingredient collocation in each component is coordinated, create synergy, nutriment is balanced, and active constituent content is even, diversified, Be very effective in raising Cynoglossus semilaevis survival rate, can not develop immunity to drugs and the problem such as medicament residue.
Detailed description of the invention
Below by specific embodiment, technical scheme of the present invention is described in further detail.Should be appreciated that enforcement of the present invention is not limited to the following examples, any pro forma accommodation make the present invention and/or change all will fall into scope.
In the present invention, if not refer in particular to, all parts, percentage are unit of weight, and all equipment and raw material etc. all can be buied from market or the industry is conventional.Compound premix in the present embodiment is the commercially available product of Chengdu Tongwei Co., Ltd., also according to circumstances can select the product of other producers.
Embodiment 1:
A, to mix through Radix Acanthopanacis Senticosi root 4g, the ampelopsis grossdentata 8g of super-dry process, agate card 12g and folium cortex eucommiae 16g, after pulverizing, cross 200 mesh sieves;
B, Fructus Hordei Germinatus 5g pulverize slurrying with bamboo vinegar 65g mixing immersion after 10 hours;
The slurry that c, the powder obtained by step a and step b obtain mixes and adds beta glucan 0.2g and Kluyveromyces fragilis 15g, fully obtains Cynoglossus semilaevis special immune reinforcing agent product after mixing.Product also can make the particle of about 2.5mm through granulator, obtain granular immunopotentiator after forced air drying.
Embodiment 2:
A kind of Cynoglossus semilaevis special immune reinforcing agent, concrete preparation method is see embodiment 1, and its proportioning is: beta glucan 0.4g, Radix Acanthopanacis Senticosi root 12g, ampelopsis grossdentata 10g, agate card 10g, Kluyveromyces fragilis 12g, folium cortex eucommiae 10g, Fructus Hordei Germinatus 10g, bamboo vinegar 80g.
Embodiment 3:
A kind of Cynoglossus semilaevis special immune reinforcing agent, concrete preparation method is see embodiment 1, and its proportioning is: beta glucan 0.2g, Radix Acanthopanacis Senticosi root 8g, ampelopsis grossdentata 5g, agate card 15g, Kluyveromyces fragilis 15g, folium cortex eucommiae 20g, Fructus Hordei Germinatus 10g, bamboo vinegar 70g.
Embodiment 4:
A kind of Cynoglossus semilaevis special immune reinforcing agent, concrete preparation method is see embodiment 1, and its proportioning is: beta glucan 0.2g, Radix Acanthopanacis Senticosi root 6g, ampelopsis grossdentata 8g, agate card 10g, Kluyveromyces fragilis 15g, folium cortex eucommiae 18g, Fructus Hordei Germinatus 8g, bamboo vinegar 70g.
Embodiment 5:
A kind of Cynoglossus semilaevis special immune reinforcing agent, concrete preparation method is see embodiment 1, and its proportioning is: beta glucan 0.4g, Radix Acanthopanacis Senticosi root 8g, ampelopsis grossdentata 5g, agate card 15g, Kluyveromyces fragilis 15g, folium cortex eucommiae 20g, Fructus Hordei Germinatus 5g, bamboo vinegar 65g.
propagate experiment artificially:
Choose that initial weight is within the scope of 20-25g, the Cynoglossus semilaevis fry of long about the 15cm of body, test is divided into 6 groups, number by 1. to 6., it is 6. wherein blank group, 1. the obtained Cynoglossus semilaevis special immune reinforcing agent double sliding-tongue sole seedling of embodiment of the present invention 1-5 is used to cultivate to 5. organizing, the consumption of immunopotentiator is 0.6% of mixed fodder gross weight, and control group is not fed immunopotentiator.Often organize 2 parallel, eachly parallelly put into 20 tail fries, culturing time is 10 months, and feeding effect is in table 1.Duration of test, adopt the mixed fodder of conventional commercial to feed to each group of fry, each group cultivates under the same test conditions, keeps inflation, and sooner or later respectively changes water once.
Table 1
Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
The mean feed | 0.80 | 1.04 | 1.02 | 1.05 | 1.00 | 1.01 |
Feed coefficient reduces (%) | 0.24 | 0.22 | 0.25 | 0.02 | 0.21 | |
Average survival | 69% | 91% | 89% | 90% | 88% | 89% |
Survival rate improves (%) | 22 | 20 | 21 | 19 | 20 |
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.
Claims (6)
1. a Cynoglossus semilaevis special immune reinforcing agent, is characterized in that it is mixed by the component of following weight portion: beta glucan 0.2-0.4 part, Radix Acanthopanacis Senticosi root 4-12 part, ampelopsis grossdentata 5-10 part, agate card 10-15 part, Kluyveromyces fragilis 12-15 part, folium cortex eucommiae 10-20 part, Fructus Hordei Germinatus 5-10 part, bamboo vinegar 65-80 part.
2. Cynoglossus semilaevis special immune reinforcing agent according to claim 1, is characterized in that: described bamboo vinegar is that the vaporization liquid of bamboo wood high-temperature heating decomposition is through cooled product.
3. Cynoglossus semilaevis special immune reinforcing agent according to claim 1 and 2, is characterized in that it is mixed by the component of following weight portion: beta glucan 0.2-0.4 part, Radix Acanthopanacis Senticosi root 4-6 part, ampelopsis grossdentata 8-10 part, agate card 10-12 part, Kluyveromyces fragilis 12-15 part, folium cortex eucommiae 18-20 part, Fructus Hordei Germinatus 5-8 part, bamboo vinegar 65-70 part.
4. a preparation method for Cynoglossus semilaevis special immune reinforcing agent according to claim 1, is characterized in that the method comprises the steps:
A, by through the mixing of the Radix Acanthopanacis Senticosi root of super-dry process, ampelopsis grossdentata, Ma Ka and folium cortex eucommiae, after pulverizing, cross 150-200 mesh sieve;
B, Fructus Hordei Germinatus mix with bamboo vinegar soak 10-12 hour after pulverize slurrying;
The slurry that c, the powder obtained by step a and step b obtain mixes and adds beta glucan and Kluyveromyces fragilis, fully obtains product after mixing.
5. the preparation method of Cynoglossus semilaevis special immune reinforcing agent according to claim 4, is characterized in that: described ampelopsis grossdentata is the young young stem and leaf of ampelopsis grossdentata plant.
6. Cynoglossus semilaevis special immune reinforcing agent according to claim 1 is preparing the application in feed, it is characterized in that: the described addition of Cynoglossus semilaevis special immune reinforcing agent in feed is 0.2-4wt%.
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CN104522425A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for adult fish of cynoglossus semilaevis and preparation method of granular feed |
CN104522426A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Pellet feed for tongue sole juvenile fishes and preparation method of pellet feed |
CN104543528A (en) * | 2014-12-30 | 2015-04-29 | 青岛农业大学 | Cynoglossus semilaevis larva pellet feed and preparation method |
CN104605166A (en) * | 2015-03-05 | 2015-05-13 | 商巧玲 | Composition for improving laying rate of quails |
CN106036232B (en) * | 2016-06-30 | 2019-08-23 | 广东省农业科学院蚕业与农产品加工研究所 | Ampelopsis grossedentata extrat is in the application quickly gone in removing fishy odor |
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CN101757604A (en) * | 2008-12-11 | 2010-06-30 | 天津瑞贝特科技发展有限公司 | Immune polysaccharide intensifier and preparation method and application thereof |
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