CN101757604A - Immune polysaccharide intensifier and preparation method and application thereof - Google Patents
Immune polysaccharide intensifier and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to an immune polysaccharide intensifier, and the components of the intensifier and the mass percentages are as follows: 1-40 percent of beta-glucan, 1-40 percent of mannan-oligosaccharide, 1-40 percent of immune regulate factor, and 1-40 percent of poly-isomaltose. The intensifier can increase the resistance to pathogenic microbes and nonspecific defense mechanism of aquaculture animals, has high safety, small effect on environmental pollution, no residue, growth-promoting capacity, convenient use and low cost and is the immune polysaccharide intensifier which can improve physique of the aquaculture animals effectively.
Description
Technical field
The invention belongs to field of veterinary, especially a kind of immune polysaccharide intensifier and preparation method thereof, application.
Background technology
When culture fishery is worldwide flourish,, and cause ecological deterioration, cause the frequent generation of all kinds of bacterial diseases, virosis, seriously hindered the sustainable and healthy development of culture fishery owing to reasons such as high density intensive culture, excessive bait throwing in.Large tracts of land death all took place in Fish, shellfish, shrimps, Eriocheir sinensis class, and especially the mortality rate of some viral disease causes destructive strike for the Aquatic product aquaculture up to 60%～100%.At present, the control aquatic animal disease mainly contains 3 kinds of methods: chemotherapy, vaccination and immunostimulation.Chemotherapy mainly based on antibiotic and other chemicalses, is used for avoiding and controlling the actute infection of Fish, but because problems such as drug residue, environmental pollution, resistance are restricted its application; Vaccination can effectively reduce the danger of aquatic animal outburst disease, but existing vaccine specific is stronger, does not still have a kind of vaccine that can successfully manage multiple pandemic; The immunostimulation method is mainly used immunostimulant, it can strengthen the aquatic animal non-specific immunity and in various degree to the resistance of disease, and, immunostimulant can remedy the deficiency of chemotherapy and vaccination, the exploitation medicament for immunity enhancement more and more is subject to people's attention, improve the body constitution of aquatic animal with immunologic method, strengthen them to the resistance of pathogenic microorganism a kind of good method of can yet be regarded as, increasing Chinese scholars is furtherd investigate, and some are pollution-free in the expectation searching, noresidue, growth promotion, multi-functional immunostimulant.
Immunostimulant (Immunostimulants) claims immunopotentiating agent again, is meant to have promotion or bring out host defense, strengthens a class material of living organism resistance against diseases.Immunostimulant mainly acts on the nonspecific immunity mechanism of aquatic animal, thereby the immune function that activates body self reaches protection effect, strengthens its premunition.Existing immunostimulant mainly contains cell composition material type, bioactive substance, Chinese herbal and crude drugs preparations class of chitin, polysaccharide, protein, hormone, vitamins, antibacterial and nutrient substance such as organic selenium and the vitamin E etc. that some are micro-.Though the application starting of these immunostimulants on China's aquaculture is late, development rapidly.Find that through retrieval the patent No. is 200410084410.5, name is called the patent of invention of a kind of river crab used immunopotentiator and preparation method thereof, notification number is CN1631206, this immunostimulant is a kind of composite immune reinforcing agent that contains vitamin C, E etc. and some polysaccharide composition, this immunostimulant contains antioxidative material vitamin E, if be added in the feedstuff of aquatic animal, As time goes on and gradually its oxidation resistance certainly will weaken or disappear, and then influences drug effect; Another part patent No. through retrieval is 200410035416.3, name is called the patent of invention of Bao compound Chinese herb immunostimulant, notification number is CN1593567, and this patent of invention has proposed with the immunostimulant of compound Chinese herb as Carnis Haliotidis, but Chinese herbal medicine class material can not directly use, need particular process, just can add in the feedstuff, allow aquatic animal ingest, thereby bring into play its biology performance, the quality of process technology directly has influence on the performance of Chinese herbal medicine, course of processing complexity; Another part application number through retrieval is 200710031733.1, name is called the application for a patent for invention of the new fine jade oligosaccharide of a kind of activity as the application of immunostimulant in aquaculture, publication number is CN101181014, this application file proposes the new fine jade oligosaccharide of a kind of activity and uses in aquaculture as immunostimulant, this immunostimulant is all feed additive, thereby has improved the cost of aquaculture.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of immune polysaccharide intensifier is provided, this reinforcing agent can improve resistance and the nonspecific defense mechanism of aquatic animal to pathogenic microorganism, safety is higher, the influence of environmental pollution is less, noresidue, growth promotion, easy to use, cost is lower, and can effectively improve the body constitution of aquatic animal.
Another object of the present invention is to provide a kind of preparation method of immune polysaccharide intensifier.
Another object of the present invention is to provide the application of a kind of immune polysaccharide intensifier in the aquaculture feed of preparation prevention aquatic animal disease.
Another object of the present invention is to provide the application of a kind of immune polysaccharide intensifier in the aquaculture feed of preparation treatment aquatic animal disease.
The present invention is achieved by the following technical solutions:
A kind of immune polysaccharide intensifier, the component of this reinforcing agent and percentage by weight thereof are:
Beta glucan 1～40%;
Mannooligo saccharide 1～40%;
Immunoregulatory factor 1～40%;
Poly-dextrinose 1～40%.
And described immunoregulatory factor component and percentage by weight thereof are:
FK-565???????????????????????10～50％；
MDP??????????????????????????10～50％。
And the albumen total content in the described immune polysaccharide intensifier is N, and the span of N is 1≤N≤5%.
A kind of preparation method of immune polysaccharide intensifier, the step of this preparation method is as follows:
(1). after taking by weighing levamisole hydrochloride, FK-565 and MDP mix homogeneously by proportioning, promptly obtain immunoregulatory factor;
(2). take by weighing beta glucan, mannooligo saccharide, immunoregulatory factor and oligomeric isomaltose and mix homogeneously by proportioning, promptly get immune polysaccharide intensifier.
The application of a kind of immune polysaccharide intensifier in the aquaculture feed of preparation prevention aquatic animal disease adds the Xg immune polysaccharide intensifier in wherein every 100Kg aquaculture feed, the span of X is 20≤X≤80.
And described aquaculture feed is one of them a feedstuff of fish, shrimp, Eriocheir sinensis, Anguillar japonica, Stichopus japonicus, Trionyx sinensis Wiegmann.
The application of a kind of immune polysaccharide intensifier in the aquaculture feed of preparation treatment aquatic animal disease adds the Yg immune polysaccharide intensifier in wherein every 100Kg aquaculture feed, the span of Y is 80＜Y≤150.
And described aquaculture feed is one of them a feedstuff of fish, shrimp, Eriocheir sinensis, Anguillar japonica, Stichopus japonicus, Trionyx sinensis Wiegmann.
Advantage of the present invention and beneficial effect are:
1. be added with beta glucan (β-glucan) in the component of this immune polysaccharide intensifier, it is not only the material of constructing multiple biological cell wall, and between biology, also demonstrate various biological activitys in the interactional process, and can determine the vaccine antigen specificity.
2. be added with mannooligo saccharide in the component of this immune polysaccharide intensifier, it has with the receptor of noxious bacteria strives bonded ability unexpectedly, thereby stop antibacterial to combine with animal intestinal is epithelial, quickening antibacterial discharges from intestinal, promote the balance of intestinal normal flora, regulate human body immune function simultaneously, and then reach growth promotion and the Nutrition that strengthens premunition.
3. this immune polysaccharide intensifier can be oral, also can sprinkle in addition, has easy to use, the diversified characteristics of method.
4. be added with levamisole hydrochloride, FK-565, MDP, oligomeric isomaltose in the component of this immune polysaccharide intensifier, can reduce or exempt antibiotic use after adding according to a certain ratio, improve the nonspecific defense mechanism of aquatic animal, improve the aquatic food quality safety, minimizing is to the pollution of environment, and can be used as the immunostimulant of all kinds of different aquatic animals, also can be used for the treatment of common disease, improve the anti-infection ability of aquatic animal, thereby improve the quantum of output of unit breeding water body.
5. the present invention can improve resistance and the nonspecific defense mechanism of aquatic animal to pathogenic microorganism, safety is higher, the influence of environmental pollution is less, noresidue, growth promotion, easy to use, cost is lower, is a kind of immune polysaccharide intensifier that can effectively improve aquatic animal body constitution.
Description of drawings
Fig. 1 be add in the feedstuff immune polysaccharide intensifier to counteracting toxic substances after the influence of tilapia mortality rate.
The specific embodiment
The present invention is described in further detail by following examples.Need to prove: following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Raw material beta glucan among the present invention, mannooligo saccharide, poly-dextrinose, levamisole hydrochloride, FK-565, MDP all can buy on market and obtain.
Embodiment 1
A kind of immune polysaccharide intensifier, its component and weight thereof are:
Beta glucan is that 40Kg, mannooligo saccharide are that 40Kg, levamisole hydrochloride are 6Kg, and FK-565 is 3Kg, and MDP is 1Kg, poly-dextrinose 10Kg, and wherein the albumen total content of this immune polysaccharide intensifier is 5%.
Albumen total content in the immune polysaccharide intensifier is controlled at 5%.
A kind of preparation method of immune polysaccharide intensifier, the step of this preparation method is as follows:
(1). after taking by weighing levamisole hydrochloride, FK-565 and MDP mix homogeneously by said ratio, promptly obtain immunoregulatory factor;
(2). take by weighing beta glucan, mannooligo saccharide and oligomeric isomaltose and add the immunoregulatory factor mix homogeneously by said ratio, promptly get immune polysaccharide intensifier.
Embodiment 2
A kind of immune polysaccharide intensifier, its component and weight thereof are:
Beta glucan is that 25Kg, mannooligo saccharide are that 25Kg, levamisole hydrochloride are 4Kg, and FK-565 is 8Kg, and MDP is 8Kg, poly-dextrinose 30Kg, and wherein the albumen total content of this immune polysaccharide intensifier is 4%.
Albumen total content in the immune polysaccharide intensifier is controlled at 4%.
The step of its preparation method is with embodiment 1.
Embodiment 3
A kind of immune polysaccharide intensifier, its component and weight thereof are:
Beta glucan is that 15Kg, mannooligo saccharide are that 15Kg, levamisole hydrochloride are 8Kg, and FK-565 is 16Kg, and MDP is 16Kg, poly-dextrinose 30Kg, and wherein the albumen total content of this immune polysaccharide intensifier is 3%.
Albumen total content in the immune polysaccharide intensifier is controlled at 3%.
The step of its preparation method is with embodiment 1.
Embodiment 4
A kind of immune polysaccharide intensifier, its component and weight thereof are:
Beta glucan is that 40Kg, mannooligo saccharide are that 10Kg, levamisole hydrochloride are 9Kg, and FK-565 is 9Kg, and MDP is 12Kg, poly-dextrinose 20Kg, and wherein the albumen total content of this immune polysaccharide intensifier is 2%.
Albumen total content in the immune polysaccharide intensifier is controlled at 2%.
The step of its preparation method is with embodiment 1.
Embodiment 5
A kind of immune polysaccharide intensifier, its component and weight thereof are:
Beta glucan is that 10Kg, mannooligo saccharide are that 40Kg, levamisole hydrochloride are 1Kg, and FK-565 is 5Kg, and MDP is 4Kg, poly-dextrinose 40Kg, and wherein the albumen total content of this immune polysaccharide intensifier is 1%.
Albumen total content in the immune polysaccharide intensifier is controlled at 1%.
The step of its preparation method is with embodiment 1.
The application of a kind of immune polysaccharide intensifier in the aquaculture feed of preparation prevention aquatic animal disease adds the Xg immune polysaccharide intensifier in wherein every 100Kg aquaculture feed, the span of X is 20≤X≤80.This aquaculture feed is one of them a feedstuff of fish, shrimp, Eriocheir sinensis, Anguillar japonica, Stichopus japonicus, Trionyx sinensis Wiegmann.
Embodiment 1
In 100kg Penaeus vannamei complete feed, add above-mentioned immune polysaccharide intensifier 20g, be used to prepare the breeding feed of prevention Penaeus vannamei disease.
Embodiment 2
In 100kg fresh-water fishes complete feed, add above-mentioned immune polysaccharide intensifier 65g, be used to prepare the breeding feed of prevention fresh-water fishes disease.
Embodiment 3
In 100kg crab complete feed, add above-mentioned immune polysaccharide intensifier 55g, be used to prepare the breeding feed of prevention crab disease.
Embodiment 4
In 100kg soft-shelled turtle complete feedstuff, add above-mentioned immune polysaccharide intensifier 80g, be used to prepare the breeding feed of prevention Trionyx sinensis Wiegmann disease.
Embodiment 5
In 100kg Anguillar japonica complete feed, add above-mentioned immune polysaccharide intensifier 70g, be used to prepare the breeding feed that prevents disease of eel.
The application of a kind of immune polysaccharide intensifier in the aquaculture feed of preparation treatment aquatic animal disease adds the Yg immune polysaccharide intensifier in wherein every 100Kg aquaculture feed, the span of Y is 80＜Y≤150.This aquaculture feed is one of them a feedstuff of fish, shrimp, Eriocheir sinensis, Anguillar japonica, Stichopus japonicus, Trionyx sinensis Wiegmann.
Embodiment 1
In 100kg Penaeus vannamei complete feed, add above-mentioned immune polysaccharide intensifier 100g, be used to prepare the breeding feed of treatment Penaeus vannamei disease.Threw something and fed continuously 5～7 days is a course of treatment.
Embodiment 2
In 100kg fresh-water fishes complete feed, add above-mentioned immune polysaccharide intensifier 120g, be used to prepare the breeding feed of treatment fresh-water fishes disease.Threw something and fed continuously 5～7 days is a course of treatment.
Embodiment 3
In 100kg crab complete feed, add above-mentioned immune polysaccharide intensifier 110g, be used to prepare the breeding feed of treatment crab disease.Threw something and fed continuously 5～7 days is a course of treatment.
Embodiment 4
In 100kg soft-shelled turtle complete feedstuff, add above-mentioned immune polysaccharide intensifier 150g, be used to prepare the breeding feed of treatment Trionyx sinensis Wiegmann disease.Threw something and fed continuously 5～7 days is a course of treatment.
Embodiment 5
In 100kg Anguillar japonica complete feed, add above-mentioned immune polysaccharide intensifier 81g, be used to prepare the breeding feed for the treatment of disease of eel.Threw something and fed continuously 5～7 days is a course of treatment.
Clinical trial of the present invention
1, prophylactic tria
1.1 test method:
Select healthy Carnis Pseudosciaenae uniformly to be divided into 4 groups at random, the content of this immune polysaccharide intensifier is followed successively by 0,0.5g/kg feedstuff, 1.0g/kg feedstuff, 1.5g/kg feedstuff, undertaken by normal feeding and management program, first group is matched group, the basic grain ration of feeding, Carnis Pseudosciaenae the 2nd～4 group (basic, normal, high dosage group) is feed the respectively feedstuff of the immune polysaccharide that contains above-mentioned concentration of experimental group, and experimental period is 30 days.
After the experiment of throwing something and feeding finished, to the hungry 24h of experiment fish, with Oleum Caryophylli (1: 10000) anesthesia, counting was weighed then.Randomly draw the experiment fish respectively, get blood from the tail vein with asepsis injector.Every tail experiment fish gained blood is divided into two parts, the blood that adds anticoagulant heparin is used for the analysis of leukocytes phagocytic vigor, anticoagulant blood is not put into 4 ℃ of refrigerators, treats 4 ℃ of centrifugalize serum after its blood coagulation, is used for the mensuration of serum lysozyme vigor and alternative complement pathway vigor.
Throw something and feed after the off-test, carry out the bacterial infection test, and the mortality rate of record challenge test 10d.
1.2 experimental result
1.2.1 feeding experiment result
Experimental period, measured non-specificity index, rate of body weight gain, the survival rate index of Carnis Pseudosciaenae, the results are shown in Table 1.The non-specificity index that adds immune polysaccharide intensifier experimental group tilapia blood in the feedstuff all is significantly higher than matched group; And between two three interpolation levels (0.5gkg-1 feedstuff, 1.0gkg-1 feedstuff, 1.5gkg-1 feedstuff) the non-specificity index significant difference of tilapia.
Table 1 immune polysaccharide is to the influence of tilapia immune indexes and rate of body weight gain, survival rate
| Group | Serum lysozyme activity (U/mL) | WBC counting (10 6/mL) | Phagocytic rate % | Complement activity (U/mL) | Rate of body weight gain % | Survival rate % |
| Matched group | ??1.10 | ??6.10 | ??46 | ??5.0 | ??71 | ??85 |
| Low dose group | ??1.40 | ??7.38 | ??64 | ??7.13 | ??93 | ??96 |
| Middle dosage group | ??1.43 | ??7.45 | ??67 | ??7.19 | ??98 | ??97 |
| High dose group | ??1.41 | ??7.32 | ??65 | ??7.12 | ??97 | ??98 |
1.2.2 challenge test result
Viewing test result shows that the Carnis Pseudosciaenae of the immune polysaccharide of not throwing something and feeding begins dead after 60 hours in bacterial injection, and the feedstuff that contains immune polysaccharide intensifier of throwing something and feeding is postponed dead phenomenon as each group demonstration of enhancing human body immunity power.Behind the antibacterial challenge test 10d, matched group tilapia cumulative mortality is 64.3%, be significantly higher than and add immune polysaccharide feedstuff experimental group (25.7%, 22.7% and 26.3%), as shown in Figure 1, cumulative mortality difference occurs between three interpolation levels, be good with middle dosage (1.0gkg-1 feedstuff).
2, therapeutic test
112 mu in big yellow castle village, the big yellow castle of Tianjin Wuqing District township crawl, fish rotten cheek, red skin, common infection disease such as hemorrhage appear in 1 meter of the depth of water.
Analyze reason: along with temperature raises, it is very fast that sashimi (raw fish) is grown, each pathogenic bacterium accelerates multiplication, food ration because of fish increases in addition, causes daily ration, feeding quantity to increase, and excretory feces increases, water scarcity, to such an extent as to can not well change water, cause every index bacterial multiplication of water body, to such an extent as to the infection disease has appearred in fish.
Treatment: by the immune polysaccharide intensifier of per hundred kilograms of feed-mixings 100 gram feed or make dissolving with the immune polysaccharide intensifier of every mu of rice 40g after carry out outer sprinkling, use after 5～7 days, symptom disappears substantially, cure rate has improved more than 20%, use through users, sickness rate has reduced by 10%.
The characteristic of medicine
1, immune polysaccharide (Immune folysaccharide) typically refers to and can determine the specific part of vaccine antigen, and this material is distributed in fungus, antibacterial and the plant widely.(β-glucan) is not only the material of constructing multiple biological cell wall, and also demonstrates various biological activitys between biology in the interactional process as beta glucan.The beta glucan of using in this patent is mainly lentinan.
Lentinan (Lentinan) is a kind of mixture, constitute by the polysaccharide more than six kinds, comprise in 4 glucosan in the heteropolysaccharide and 2, its bioactive basic substance is a beta glucan, its structure is to be main chain with β-(1 → 3)-D-glucoside bond, is ramose glucosan with β-(1 → 6)-D-glucose Glucopyranose..The molecular structure such as the table 2 of lentinan, primary structure such as structural formula 1 belong to beta glucan.
The molecular structure of table 2. lentinan (Molecular structure of lentinan)
| The glycosidic bond of main chain | Side chain | Molecular weight | Degree of branching | Higher structure | |
| ??Lentinan | β-(1 → 3)-D-glucose | β-(1 → 6)-D-glucose | ??4.8×10 5 | ??2/5 | β-three is with spiral |
Structural formula 1
Lentinan is the basis of its physiologically active to the immunomodulating and the enhanced physiological function of body.Lentinan is typical t cell activation agent; the inside and outside all can promote the generation of cytotoxic T cell (CTL); improve the vigor that kills and wounds of CTL, strengthen the delayed hypersensitive reaction (DTH) of normal or immunologic hypofunction mice, improve antibody dependent cellular degree cell (ADDC) activity.Lentinan at first induces the generation acute phase protein inducible factor (APPIF) of macrophage in vivo, occurs blood vessel expand hemorrhage inducible factor (VDHIF), interleukin 1 inducible factor (IL-IPF), IL-3 (CSF) etc. subsequently in the serum.These factors remake and are used for lymphocyte, hepatocyte, vascular endothelial cell etc.Cause the generation of many immunne response of immunity and inflammation-related.The anti-tumor activity of Lentinan can be prevented by the antagonist of macrophage so think that lentinan is a kind of thymus dependent T cell guiding.Lentinan can activate the NK cell in vivo, then can not external; It is relevant with the secretion that promotes interferon (IFN) that it activates the NK cell.Lentinan can also the activating complement system in addition, and macrophage lysosome and IL-1 secretory volume are increased, and discharges PGE, PGF and suppress macrophage.
2, mannooligo saccharide claims manna oligosaccharide or grape manna oligosaccharide again, and its english abbreviation is MOS (Mannose Oligosaccharide), is a class of oligosaccharide.The MOS main chain is by mannose and glucose or galactose residue.Connect to form by α (1-2), α (1-3), α (1-6), β (1-4) or β (1-3) glycosidic bond.Big quantity research discloses, mannooligo saccharide has with the receptor of noxious bacteria strives bonded ability unexpectedly, thereby stop antibacterial to combine with animal intestinal is epithelial, quickening antibacterial discharges from intestinal, promote the balance of intestinal normal flora, regulate human body immune function simultaneously, and then reach growth promotion and the Nutrition that strengthens premunition.
3, (Levamisole LMS) is the laevoisomer of Anthelvet to levamisole, its hydrochlorate commonly used or phosphate.Levamisole hydrochloride (Levamisole Hydrochloride), (s)-(-)-6-phenyl-2,3,5, the 6-imidazolidine is [2,1-b] thiazole hydrochloride also, and its structure is seen structural formula 2.
Structural formula 2
LMS (levamisole) is imidazoles a pair of horses going side by side thiazoles anthelmintic of wide spectrum commonly used during animal produces, efficient, low toxicity, safety.Discover, it is except having anthelmintic function, also has immunoregulation effect, it mainly passes through to improve activity, the lymphocytic generation of stimulation or the secretion lymphokine of phagocyte and neutrophil cell in the aquatic animal body, improve antalzyme activity in leukocytic metabolic capacity and the serum, the generation of induce antibody and the generation of complement wait the immunity of enhancing body, can be used for the aspects such as anti-inflammation, antiviral and promotion growth of animal.
4, MDP is muramyldipeptide (N-acetylmuramyl-L-alanyl-D-isoglutamine; N-acetyl group muramyl-L-alanyl-D-isoglutamine) low molecular peptide that obtains by mycobacterium.MDP is the minimum active unit that still possesses the tuberculosis vaccines adjuvant effect on the Mycobacterium tuberculosis cell wall, molecular weight＜500.This dipeptides can activating macrophage and bone-marrow-derived lymphocyte and complement pathway as immunopotentiating agent, it and 5-HT are incorporated on the same acceptor site of macrophage, engulfing, synthesizing and secretory function by tyrosine kinase or triggering of protein kinase c approach and activated mononuclear cell.Macrophage plays a role by endocytosis and secretion series of effects molecule just.MDP can significantly promote rat abdominal cavity macrophage TNF secretion, and this material is the topmost effector molecule of macrophage performance cytotoxicity.
5, FK-565 (heptanoyly-D-glutamyl-L-meso-diaminopimelyl-D-alanine, heptanone-Υ-D-glutamic acid-L-meso-diaminourea two bases in heptan-D-alanine) is a kind of by the isolating lactoyl tetrapeptide of Streptomycesolivaceogriseus culture.It has the ability that makes animal improve anti-microbial infection.Be activated to having strengthened the cytophagous activity of rainbow trout behind the rainbow trout injection FK-565 to the opposing, particularly rainbow trout of aeromonas salmonicida (Aeromonas salmonicida).When handling rainbow trout, can stimulate rainbow trout spleen medium-sized lymphocyte to produce more multispecific antibody with the blended FK.565 of the lipopolysaccharide vaccine of salmon Aeromonas.
6, oligomeric isomaltose (Isomaltooligosacharide, abbreviation IMO) claims branch's oligosaccharide, dextrinosan, isomalto Oligosaccharide again, be meant that glucosyl group is with α～1,6 glycosidic bond be combined into monosaccharide numbers are at 2～6 class oligosaccharide that do not wait, and its main component is dextrinose (Isomaltose), panose (Panose), Isomaltotriose (Isomaltotriose) and different maltotetraose etc.Oligomeric isomaltose can promote bacillus bifidus propagation in the intestinal, suppresses harmful intestinal tract bacteria and corrupt substance and forms, and reduces the intestinal carcinogen, improves serum lipids, cholesterol reducing content; Nutrient is absorbed with facilitation and produces nutrient, increase vitamin content; Human body immunity improving power plays the immunomodulator effect; Promote food digestion, absorption, keep the intestinal normal function.Oligomeric isomaltose significantly is better than antibiotic and probiotics as additive.Can replace active bacteria formulation to be applied to the feedstuff medical industry, be used for domestic animals and fowls and aquaculture, as animal promote production agent and health-care agent.Because oligomeric isomaltose is the bacillus bifidus multiplicaiton factor, has enhancing immunity, blood fat reducing and cholesterol, functions such as hepatoprotective are applied to pharmaceutical industry.On function, work the effect of complementing each other with the immune polysaccharide coupling.
Claims (8)
1. immune polysaccharide intensifier, it is characterized in that: the component of this reinforcing agent and percentage by weight thereof are:
Beta glucan 1～40%;
Mannooligo saccharide 1～40%;
Immunoregulatory factor 1～40%;
Poly-dextrinose 1～40%.
2. immune polysaccharide intensifier according to claim 1 is characterized in that: described immunoregulatory factor component and percentage by weight thereof are:
Levamisole hydrochloride 10～60%;
FK-565??????????????????10～50％；
MDP?????????????????????10～50％。
3. immune polysaccharide intensifier according to claim 1 is characterized in that: the albumen total content in the described immune polysaccharide intensifier is N, and the span of N is 1≤N≤5%.
4. the preparation method of an immune polysaccharide intensifier as claimed in claim 1, it is characterized in that: the step of this preparation method is as follows:
(1). after taking by weighing levamisole hydrochloride, FK-565 and MDP mix homogeneously by proportioning, promptly obtain immunoregulatory factor;
(2). take by weighing beta glucan, mannooligo saccharide, immunoregulatory factor and oligomeric isomaltose and mix homogeneously by proportioning, promptly get immune polysaccharide intensifier.
5. the application of immune polysaccharide intensifier in the aquaculture feed of preparation prevention aquatic animal disease, it is characterized in that: add the Xg immune polysaccharide intensifier in every 100Kg aquaculture feed, the span of X is 20≤X≤80.
6. the application of immune polysaccharide intensifier according to claim 5 in the aquaculture feed of preparation prevention aquatic animal disease is characterized in that: described aquaculture feed is one of them a feedstuff of fish, shrimp, Eriocheir sinensis, Anguillar japonica, Stichopus japonicus, Trionyx sinensis Wiegmann.
7. the application of immune polysaccharide intensifier in the aquaculture feed of preparation treatment aquatic animal disease, it is characterized in that: add the Yg immune polysaccharide intensifier in every 100Kg aquaculture feed, the span of Y is 80＜Y≤150.
8. the application of immune polysaccharide intensifier according to claim 7 in the aquaculture feed of preparation treatment aquatic animal disease is characterized in that: described aquaculture feed is one of them a feedstuff of fish, shrimp, Eriocheir sinensis, Anguillar japonica, Stichopus japonicus, Trionyx sinensis Wiegmann.
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