CN104510729A - Animal immune resistance-activating composition of fatty acid and derivatives thereof - Google Patents
Animal immune resistance-activating composition of fatty acid and derivatives thereof Download PDFInfo
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- CN104510729A CN104510729A CN201410491273.0A CN201410491273A CN104510729A CN 104510729 A CN104510729 A CN 104510729A CN 201410491273 A CN201410491273 A CN 201410491273A CN 104510729 A CN104510729 A CN 104510729A
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Abstract
Provided is a preparation formula prepared by combining fatty acid or fatty acid salts or derivatives thereof with chitosan and the like. The composition has significant effects on enhancing animal immunity resistance, inhibiting tumor growth, enhancing an animal anti-stress ability, increasing animal appetite and promoting animal growth, and has positive significance in reduction of livestock dependence on antibiotics, hormones and the like.
Description
Technical field
The invention belongs to biological technical field, relate to for detoxification or the immune resistance reinforcing agent drunk.
Background technology
The development of modern poultry and aquaculture industry plays a very important role for China's economic development and environment sustainable development.But along with the raising of cultivation intensive degree, the density of the animal cultivation such as poultry and fish shrimp crab is large, adds a large amount of uses of hormone or antibiotic etc., animal growth rate is fast, and the ability of animal anti-stress significantly weakens, and immune resistance is low, be very easy to cause the popular of disease, cause animal mortality.
Stress under environment, the irritability of animal body feeding center reduces, neuroendocrine disorders, and the hormone secretion such as thyroxine, epinephrine is disorderly, degradation under human body immune function.Such as, pig cultivation aspect be mainly manifested in following several respects: 1) feed intake reduces, and grower pigs weightening finish is slowly.2) pig breeding dysfunction is planted.Sow hypo ovaria, miscarriage, conception rate decline, the increase of stillborn fetus number, lactation amount decline, piglet survival ratio reduction etc.; Breeding boar hyposexuality, semen quality decline, sperm quantity minimizing, vigor reduction, abnormal rate rising etc.3) disease such as the dermatitis nephrotic syndrome, baby pig diarrhoea, unknown high fever, chronic ileitis increases.4) meat quality reduces.5) virosis is popular.Then there is the poor growths such as shrimp Eriocheir sinensis, shelling difficulty, disease popularity, mortality in aquatic products.
Traditional method is mainly through adding the anti-stress ability of anti-stress additive as enhancing animals such as fat, synthesizing amino acid, electrolyte, mineral, vitamin and organic acids, or the resistance against diseases by using antibiotic and vaccine to strengthen animal, can play a role.But due to a large amount of uses of these products, not only bring the food-safety problems such as the residue of veterinary drug further received publicity, result also in the day by day serious resistance of pathogenic microorganism and produce, serious challenge is constituted to the development of aquaculture.
In recent years, some experts and scholars start to pay close attention to polysaccharose substance as the effect of anti stress immunity enhancing agent in the Immunoresistance improving animal body both at home and abroad.Domestic and international research finds, polysaccharide such as chitosan can increase the anti-stress immunity of animal, is applied in the feed additive of animal, can strengthen anti-stress ability and the immune resistance of animal.But because the application of single component acts on instability in actual applications, the effect of polysaccharide is not given prominence to, animal occurs that stress or sick rear use then do not have effect.
The compositions that fatty acid and polysaccharide component etc. are made by the present invention, can greatly active cell immunologic function,
Thus raising immunity of organisms, there is treatment animal virus disease, the effect of fungal disease, and the effect with Tumor suppression.
Summary of the invention
Select the fatty acid of one or more following formulas containing 0.01-99.9999%, their ester, their salt or derivant or their mixture:
And/or
In formula, X is H, C
1-C
31alkyl or alkenyl, 2-hydroxyethyl, 2-hydroxypropyl;
Y is H, C
1-C
4alkyl or alkenyl, metal or nonmetalloid or group;
Z is H, OH, C
1-C
4alkyl or alkenyl, metal or nonmetalloid or group;
By above-mentioned fatty acid, their ester, their salt or derivant or their mixture and following any one or Multiple components combination in any before being administered to target site or when being administered to target site, a kind of anion sulfate or sulfosalt surfactant, one or more edible essences, one or more polysaccharide, one or more inorganic or organic compound and one or more high molecular polymers strengthen animal immune resistance by having of being mixed of 0.1-95%, Tumor suppression grows, strengthen animal anti-stress ability, increase animal appetite, promote the compositions of growth of animal, the pH value of described compositions is 2.0-3.9.
The polysaccharide polymer polymer selected is including, but not limited to trehalose, starch, soluble starch, aminopolysaccharide, amino-oligosacchride, chitosan, glycosaminoglycans, chitosan, cellulose, hemicellulose, methylcellulose, sodium carboxymethyl cellulose, Cordyceps polysaccharide, astragalus polysaccharides, ginseng polysaccharide, Radix Notoginseng polysaccharide, dictyophora fungus polysaccharide, ganoderan, lentinan, zymosan etc.
The fatty acid selected and fatty acid ester or derivant are the saturated or undersaturated fatty acids from C2 to C28, including, but not limited to acetic acid, and diacetic acid, butanoic acid, isopropylformic acid., valeric acid, isovaleric acid, caproic acid, isocaproic acid, certain herbaceous plants with big flowers acid, sad, Palmic acid, palmitic olefinic acid, stearic acid, linoleic acid, oleic acid, linolenic acid, lauric acid, myristic acid, n-nonanoic acid, arachidonic acid etc., and the salt that these fatty acids are made.
The organic or inorganic compound selected including, but not limited to calcium chloride, sodium chloride, potassium chloride, zinc oxide, calcium oxide, calcium superphosphate, Sodium triphosphate, sodium bicarbonate, sodium polyphosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium sulfate, zinc sulfate, copper sulfate, zinc acetate, potassium sulfate, calcium carbonate, amino acid calcium, amino-acid zinc, glucosan calcium, glucosan zinc, sodium carbonate, calcium bicarbonate, potassium bicarbonate, calcium sulfate, lime nitrate, calisanin, potassium carbonate etc.
Fatty acid, their ester, their salt or derivant, polysaccharide high molecular polymer and surfactant etc. are added to the water, edible essence and inorganic or organic compound is added respectively, with inorganic or organic acid or alkali adjustment compositions pH value under constantly stirring.
The technical problem that the present invention solves is fatty acid or derivatives thereof and the polysaccharide high molecular polymer compositions made at pH2.0 to pH4.9, this compositions is for enhancing animal immune resistance, Tumor suppression grows, strengthen animal anti-stress ability, increase animal appetite, promote that growth of animal has remarkable result.
Specific embodiments
The present invention is further described according to following example, one skilled in the art will appreciate that following embodiment only plays the effect of explanation to the present invention.Without departing from the premise in the spirit of the present invention, any improvement the present invention done and substituting all in the scope of protection of the invention.
Embodiment one
Peracetic acid formulation is become 0.5% aqueous solution, getting chitosan 10g joins in 500ml 0.5% acetic acid aqueous solution, add dipotassium hydrogen phosphate 2g respectively, ammonium sulfate 5g, Sodium triphosphate 5g, glucosan calcium 8g, sodium alkyl sulfate 0.1g and stearic acid 2.5g, stir, regulate the pH value of solution to 2.9.Make compositions one.
Embodiment two
Butanoic acid is mixed with 0.5% aqueous solution, getting chitosan 10g joins in 500ml 0.5% butanoic acid aqueous solution, add potassium dihydrogen phosphate 3g, potassium sulfate 5g, Sodium triphosphate 10g, amino-acid zinc 3g, sodium alkyl sulfate 0.1g, geranyl acetate 0.5g and linolenic acid 4.6g, stirs, and regulates the pH value of solution to 3.9.Make compositions two.
Embodiment three
Choosing has the pig 30 of following symptom: gradually to become thin or growth retardation, anorexia, spirit are depressed, be slow in action, mane is fluffy and disorderly, dyspnea etc.Be divided into treatment group and matched group, two groups of affected pig ages, clinical manifestation and state of an illness weights are unanimous on the whole.Each 15 examples for the treatment of group matched group, treatment group joins in 500ml drinking water by 5ml compositions one, and every pig is pressed and supplies 500ml/ day.Matched group isolated rearing, Routine Management.The mental status, the body temperature of pig, situation of searching for food is observed every day after process.Sick pig medication clinical symptom disappearance after 5 days, the normal person of body temperature, appetite, the mental status is judged to healing; Sick pig medication is appetite, mental status improvement after 5 days, and the normal person of body temperature is judged to improvement; Taking a turn for the better and curing all is judged to effectively; Sick pig medication after 10 days still lassitude, anorexia or death be judged to invalid.Result is as table one.
The effect of table one drinking technique compositions treatment virosis pig
Compositions one joins the therapeutic effect of drinking water to sick pig and reaches 86.7%, and the sick pig of matched group has 8 death, does not still get well after 10 days for 7.Compositions one has the therapeutic effect of highly significant.
Embodiment four
Choosing has the pig 20 of virus symptoms.Be divided into treatment group and matched group, two groups of affected pig ages, clinical manifestation and state of an illness weights are unanimous on the whole.Each 10 examples for the treatment of group matched group, treatment group adds the ratio mix homogeneously of 10g compositions two in 500g feedstuff, and every day feeds.Matched group isolated rearing, conventional medicine manages.The mental status, the body temperature of pig and situation of searching for food is observed every day after process.Sick pig medication after 5 days body temperature, appetite, the normal person of the mental status be judged to healing; Appetite, the mental status take a turn for the better, the normal person of body temperature is judged to improvement; Take a turn for the better and cure and be all judged to effectively.Sick pig medication after 10 days still lassitude, anorexia or death be judged to invalid.After sick pig treats 5 days by compositions two, clinical symptoms starts to disappear, and begin to show therapeutic effect, continue the feedstuff of feeding containing compositions two, after 15 days, 8 sick pigs recover normal substantially, and effective percentage reaches 80%.
Table two feedstuff method compositions two treats the effect of virosis pig
The therapeutic effect being added compositions two by feedstuff reaches 80%, and appetite recovers normal.The sick pig of conventional medicine matched group has 5 death, does not still get well after 10 days for 3.Compositions two has the therapeutic effect of highly significant, and what promote pig takes food growth.
Embodiment five
Get Healthy female mice and be divided into 3 groups, 10/group, average every only heavy 20g.Get well-grown mouse bearing liver cancer H22 cell and be adjusted to 2 × 10 with normal saline
6the cell suspension of individual/ml concentration, is inoculated in right side of mice armpit by 0.2ml/ volume subcutaneous.Negative control group synchronously gives N.S. with tested group.Test component is high dose composition two (100mg/Kg) and low dose group compound two (50mg/Kg) two subgroups.By the administration volume gastric infusion successive administration 10 days of 0.3ml/10g, administration terminates rear next day gets tumor and weighs and calculate tumour inhibiting rate.
The impact that table three compositions two grows rat liver cancer H22
Note: gastric infusion; The each group of average tumor of mice is heavily measurement data.
The compositions gastric infusion of various dose has good inhibition to animal hepatocarcinoma.Relative to chemical anti-tumor drugs, the feature of this compositions does not affect animal appetite, and weight ratio negative control group increase about 10% when animal subject administration completes, shows that product of the present invention has good using value.
Embodiment six
Select 2, the Eriocheir sinensis pool, 5 mu, each Eriocheir sinensis pool, be divided into compositions one to process on 2 Eriocheir sinensis pools and contrast two groups with Routine Management.Juvenile crab 6000 is thrown on each Eriocheir sinensis pool.Treatment Design is spray by the every mu of Eriocheir sinensis pool water surface compositions one that 50 liters of concentration are 2%, within interval 10-15 days, sprays once.When high temperature is sultry every day observed and recorded Eriocheir sinensis death condition.When fishing for, each Eriocheir sinensis pool Weighing respectively, calculates the rate of recapture.
The effect of table four compositions one on steamed crab
| Group | Monomer heavy (gram) | Amount of fishing (jin) | Rate of recapture % |
| Contrast | 125 | 195 | 65 |
| Compositions one | 160 | 307 | 80 |
Compositions one significantly effectively reduce high temperature sultry time the water hypoxia steamed crab that causes dead, and significantly promote steamed crab growth.
Claims (5)
1. activate the fatty acid of animal immune resistance and the compositions of derivant thereof, it is characterized in that, said composition is obtained by the compound method preparation comprised the steps:
1) fatty acid of one or more following formulas containing 0.01-99.9999%, their ester, their salt or derivant or their mixture is selected:
And/or
In formula, X is H, C
1-C
31alkyl or alkenyl, 2-hydroxyethyl, 2-hydroxypropyl;
Y is H, C
1-C
4alkyl or alkenyl, metal or nonmetalloid or group;
Z is H, OH, C
1-C
4alkyl or alkenyl, metal or nonmetalloid or group;
2) by step 1) described fatty acid, their ester, their salt or derivant or their mixture and following any one or Multiple components combination in any before being administered to target site or when being administered to target site, a kind of anion sulfate or sulfosalt surfactant, one or more edible essences, one or more polysaccharide high molecular polymers, one or more inorganic or organic compound strengthen animal immune resistance by having of being mixed of 0.1-95%, Tumor suppression grows, strengthen animal anti-stress ability, increase animal appetite, promote the compositions of growth of animal, the pH value of described compositions is 2.0-4.9.
2. compositions according to right 1, is characterized in that, described fatty acid including, but not limited to acetic acid, diacetic acid, butanoic acid, isopropylformic acid., valeric acid, isovaleric acid, caproic acid, isocaproic acid, certain herbaceous plants with big flowers acid, sad, Palmic acid, palmitic olefinic acid, stearic acid, linoleic acid, oleic acid, linolenic acid, lauric acid, myristic acid, n-nonanoic acid, arachidonic acid etc., and the salt that these fatty acids are made.
3. compositions according to right 1, is characterized in that, described polysaccharide high molecular polymer is including, but not limited to trehalose, starch, soluble starch, aminopolysaccharide, amino-oligosacchride, chitosan, glycosaminoglycans, chitosan, cellulose, hemicellulose, methylcellulose, sodium carboxymethyl cellulose, Cordyceps polysaccharide, astragalus polysaccharides, ginseng polysaccharide, Radix Notoginseng polysaccharide, dictyophora fungus polysaccharide, ganoderan, lentinan, zymosan etc.
4. compositions according to right 1, is characterized in that, described inorganic or organic compound including, but not limited to calcium chloride, sodium chloride, potassium chloride, zinc oxide, calcium oxide, calcium superphosphate, Sodium triphosphate, sodium bicarbonate, sodium polyphosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium sulfate, zinc sulfate, copper sulfate, zinc acetate, potassium sulfate, calcium carbonate, amino acid calcium, amino-acid zinc, glucosan calcium, glucosan zinc, sodium carbonate, calcium bicarbonate, potassium bicarbonate, calcium sulfate, lime nitrate, calisanin, potassium carbonate etc.
5. compositions according to right 1, it is characterized in that, fatty acid, their ester, their salt or derivant, polysaccharide high molecular polymer and surfactant etc. are added to the water, under constantly stirring, add edible essence and inorganic or organic compound respectively, regulate the pH value of compositions to 2.0-4.9 with inorganic or organic acid or alkali.
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| CN109180828A (en) * | 2018-08-10 | 2019-01-11 | 成都澳立生态科技发展有限公司 | A kind of bigcatkin willow glycan ester and its application |
| CN108990983A (en) * | 2018-08-31 | 2018-12-14 | 成都特普生物科技股份有限公司 | A kind of sustained release microbial bacterial agent |
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