CN1608487A - Feed additive containing chitin oligose and its prepn and use - Google Patents

Feed additive containing chitin oligose and its prepn and use Download PDF

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Publication number
CN1608487A
CN1608487A CNA200310105427XA CN200310105427A CN1608487A CN 1608487 A CN1608487 A CN 1608487A CN A200310105427X A CNA200310105427X A CN A200310105427XA CN 200310105427 A CN200310105427 A CN 200310105427A CN 1608487 A CN1608487 A CN 1608487A
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CN
China
Prior art keywords
enzymolysis
liquid
chitosan
chinese medicine
chitosan oligosaccharide
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Application number
CNA200310105427XA
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Chinese (zh)
Inventor
赵连军
李兴福
王茂霞
陈雯雯
Original Assignee
济南海得贝海洋生物工程有限公司
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Priority to CNA200310105427XA priority Critical patent/CN1608487A/en
Publication of CN1608487A publication Critical patent/CN1608487A/en

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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The present invention is feed additive containing chitin oligose and its preparation and use. Chitin oligose is prepared with chitosan and through dissolving in acid solution to obtain chitosan solution; heating chitosan solution at 30-60 deg.c, regulating pH value to 5-6, adding composite enzyme for enzymolysis of 2-5 hr to obtain enzymolyzed liquid; filtering to eliminate zymoprotein and other impurity to obtain clear enzymolyzed liquid; concentrating the enzymolyzed liquid to obtain concentrated liquid; spray drying to obtain solid chitin oligose. The obtained solid chitin oligose is mixed with Chinese medicinal materials and crushed to obtain the additive. The additive has bacteriostasis function, no toxic side effect, no residue of harmful matter and no drug resistance, and may be used in various feeds for farm animal and aquatic animal to raise feed utilization, raise product quality and strengthen immunity.

Description

A kind of feed addictive that contains chitosan oligosaccharide and its production and use
Technical field
The present invention relates to a kind of feed addictive that contains chitosan oligosaccharide and its production and use, more particularly, the invention relates to the composition and method of making the same and the purposes of chitosan oligosaccharide and Chinese medicine.
Background technology
20 beginnings of the century, people just take to the research of feed addictive.Traditional feed addictive is each seed amino acid, antibiotic and new synthetic drug and hormone etc., and these feed addictives have promoted feed industry and aquaculture, Developing of Animal Industry all sidedly, and have formed a special industry.But these traditional feed addictives exist not enough, many negative effects have been produced, antibiotic, hormone and other synthetic drugs in human common cancer, deformity, the resistance to the action of a drug and some intoxicating phenomenon and meat, milk, the egg residual relevant, and use these additives also can make local flavor, the quality decline of livestock products for a long time.
Summary of the invention
The present invention has overcome the shortcoming of prior art, and a kind of natural nonreactive multifunctional feed additive and its production and use is provided.This product has bacteriostasis, has no side effect, do not have residual, the no resistance to the action of a drug of nuisance, improves efficiency of feed utilization and product quality, can strengthen body's immunity.
The chitosan oligosaccharide that uses among the present invention (Chitosan oligosaccharide), also claim chitin oligo saccharide, formal name used at school is β-1,4-oligomerization-gucosamine, be to be raw material with the shitosan, the low molecular weight product that good water solubility, function are big, biologically active is high that forms through biotechnology degraded.Its essential characteristic is as follows:
Outward appearance Appearance Pale yellow powder Light yellow powder
Granularity Particle >80 order 80Mesh
Viscosity Viscosity (5%) ??<10CPS
Molecular weight Molecular weight ??<5000;
??PH(1%) ??3-6
Studies have shown that: chitosan oligosaccharide has the immunity of raising, suppress the growth of cancerous swelling cell, promote liver spleen antibody to form, promote the absorption of calcium and mineral matter, profitable strains such as propagation Bifidobacterium, lactic acid bacteria, reducing blood lipid, hypotensive, hypoglycemic, adjusting cholesterol, fat-reducing, functions such as prevent disease can be applicable to fields such as medicine, functional food, farming and animal husbandry.
Product of the present invention can prepare with following method: (1) puts into acid solution with shitosan, stirs, and gets the liquid chitosan solution; Acid solution can be selected from lactic acid, acetic acid, hydrochloric acid etc., acid concentration 0.5%-2% (2) is warming up to 30-60 ℃ with chitosan solution, transfer pH value to reach 5-6, add complex enzyme, stir, this complex enzyme is made up of protease, elastoser, amylase, lipase, lysozyme, and its percentage by weight is 25-35%: 5-15%: 5-15%: 25-35%: 15-25%.The weight ratio of complex enzyme and chitosan oligosaccharide is 5-10%.Enzymolysis 2-5 hour, get enzymolysis liquid; (3) enzymolysis liquid is filtered, remove impurity such as zymoprotein, get the enzymolysis clear liquid; (4) the enzymolysis clear liquid sent in the inspissator concentrated, concentrate; (5) concentrate squeezed into carried out spray-drying in the spray dryer, the solid shell oligosaccharides; (6) the solid shell oligosaccharides ground with Chinese medicine mix, additive.Chinese medicine is ten plurality of Chinese such as folium isatidis, the coptis, Radix Isatidis, Japanese anemone, pine needle, paulownia leaves, leaves of Hawthorn, Huang Mao.
This feed addictive has multiple function, can be used as multiple feed addictives such as aquatic livestock bait such as livestock feeds such as poultry feeds such as chicken, duck, goose, ox, sheep, pig, dog, fishes and shrimps.Have no side effect, do not have the resistance to the action of a drug; The animal digestion rate is 100%, can promote growth of animals or poultry, can effectively and economically improve the production performance of livestock and poultry, improves efficiency of feed utilization and product quality, the culture benefit height; Can strengthen body's immunity, can prevent animal various infectiousness (viral, bacillary) disease, can adjust body physiological function; Use the back unharmful substance residual, do not influence the animal products quality, do not influence environment for human survival with healthy; Physicochemical property or biologically active stable in properties can enter intestines and stomach effectively and play a role, and do not influence the feed palatability; Share with the other drug additive, do not take place or rare incompatibility, bacterium is difficult for developing immunity to drugs to it; Improve the animal anti-stress ability; Pollution-free, improve environment etc.
This feed addictive has been done experimental study in pig, chicken, fishes and shrimps feed, experimental results show that the productive rate of domestic animals such as to improve pig, chicken, poultry, and the effect of improvement poultry, poultry matter in addition, experimental results show that and add Antibiotic Additive and compare, be used for pork pig feed lean meat percentage and can improve more than 12%, improve the quality of meat, improve the nutritive value of meat, the piglet daily gain improves 7.32%, and feedstuff-meat ratio reduces by 5.67%.Adopt the pig of this feed addictive feed, honey stomach, fur is bright and clean, the colour of skin is ruddy, brawniness, ight soil are normal; Be used for milk cow forage, can improve the output of milk more than 17%; Be used for the chicken feed, can improve laying rate more than 13%, can make the fryer daily gain improve 1.3%, feedstuff-meat ratio reduces by 3.15%.Be used for the fishes and shrimps feed, can make fishes and shrimps look faster, more fertile, improved the output of fishes and shrimps.
The main raw material(s) of this feed addictive derives from shrimp crab shell, and through deacetylated product, raw material sources are abundant, and cost is low nontoxic, biodegradable, good biocompatibility.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Shitosan is put into certain density acid solution, and acid solution can be selected from lactic acid, acetic acid, hydrochloric acid etc., and acid concentration 0.5%-2% under agitation dissolves, and dissolving finishes, and gets the liquid chitosan solution; Chitosan solution is warming up to 30-60 ℃, with 40 ℃ be the best, transfer pH value to reach 5--6, equaling 5 with pH value be the best, adds a certain amount of complex enzyme stirring, enzymolysis 2--5 hour, with 2 hours be the best, enzymolysis finishes, must enzymolysis liquid; Enzymolysis liquid is filtered, remove impurity such as zymoprotein, get the enzymolysis clear liquid.The enzymolysis clear liquid sent in the inspissator concentrate, be concentrated to finite concentration after, concentrate.Concentrate squeezed into carries out spray-drying in the spray dryer, the solid shell oligosaccharides.The chitosan oligosaccharide that makes by said method is molecular weight 800-1000.The solid shell oligosaccharides ground with Chinese medicine mixes, additive.Chinese medicine is ten plurality of Chinese such as folium isatidis, the coptis, Radix Isatidis, Japanese anemone, pine needle, paulownia leaves, leaves of Hawthorn, Huang Mao, and is best with folium isatidis, the coptis, Radix Isatidis, paulownia leaves, Huang Maowei.
Hydrochloric acid solution is put into shitosan in embodiment 1, (1), stirs, and gets the liquid chitosan solution; Acid concentration 0.5% (2) is warming up to 45 ℃ with chitosan solution, transfer pH value to reach 5.0, add complex enzyme, stir, this complex enzyme is made up of protease, elastoser, amylase, lipase, lysozyme, and its percentage by weight is 30%: 20%: 10%: 20%: 20%.The weight ratio of complex enzyme and chitosan oligosaccharide is 5%.Enzymolysis 2 hours gets enzymolysis liquid; (3) enzymolysis liquid is filtered, remove impurity such as zymoprotein, get the enzymolysis clear liquid; (4) the enzymolysis clear liquid sent in the inspissator concentrated, concentrate; (5) concentrate squeezed into carried out spray-drying in the spray dryer, the solid shell oligosaccharides; (6) the solid shell oligosaccharides ground with Chinese medicine mix, additive.Chinese medicine is folium isatidis, the coptis, Radix Isatidis, pine needle, paulownia leaves, Huang Mao.Chitosan oligosaccharide content is 60%, and Chinese medicine content is 40%.
Lactic acid solution is put into shitosan in embodiment 2, (1), stirs, and gets the liquid chitosan solution; Acid concentration 0.5% (2) is warming up to 45 ℃ with chitosan solution, transfer pH value to reach 5.0, add complex enzyme, stir, this complex enzyme is made up of protease, elastoser, amylase, lipase, lysozyme, and its percentage by weight is 30%: 20%: 10%: 20%: 20%.The weight ratio of complex enzyme and chitosan oligosaccharide is 5%.Enzymolysis 2 hours gets enzymolysis liquid; (3) enzymolysis liquid is filtered, remove impurity such as zymoprotein, get the enzymolysis clear liquid; (4) the enzymolysis clear liquid sent in the inspissator concentrated, concentrate; (5) concentrate squeezed into carried out spray-drying in the spray dryer, the solid shell oligosaccharides; (6) the solid shell oligosaccharides ground with Chinese medicine mix, additive.Chinese medicine is folium isatidis, the coptis, Radix Isatidis, pine needle, paulownia leaves, Huang Mao.Chitosan oligosaccharide content is 60%, and Chinese medicine content is 40%.
Embodiment 3, this feed addictive is added in piglet feed according to 0.1% ratio, the sucking pig average weight is 1.2 kilograms, feeds continuously two months.Blank group weight is 21.2 kilograms, 24.3 kilograms of control groups, blank group average weight gain 14.6%.Adopt the pig of this feed addictive feed, honey stomach, bright and clean, the brawniness of fur.
This feed addictive of embodiment 4, interpolation 0.1% in fish meal begins to feed from fry, fed continuously 5 months, and the blank group weight of carp raising 35%, survival rate improves 5%.
This feed addictive of embodiment 5, interpolation 0.2% in the meat chicken feed begins to feed from seedling, feeds the blank group reduction by 31% of cholesterol level continuously 1.5 months.

Claims (7)

1, a kind of feed addictive that contains chitosan oligosaccharide is characterized in that, the composition of this product chitosan oligosaccharide and Chinese medicine, and the content 50%-80% of chitosan oligosaccharide, the content of Chinese medicine are 20%-50%.
According to the described product of claim 1, it is characterized in that 2, the molecular weight of chitosan oligosaccharide is 800--1600, pale yellow powder, granularity>80 orders.
3, product according to claim 1 is characterized in that, Chinese medicine is selected from one or more in folium isatidis, the coptis, paulownia leaves, Huang Mao, Radix Isatidis, Japanese anemone, pine needle, the leaves of Hawthorn.
4, a kind of method for preparing the described product of claim 1 is characterized in that, may further comprise the steps: (1) puts into acid solution with shitosan, stirs, and gets the liquid chitosan solution; (2) chitosan solution is warming up to 30--60 ℃, transfers pH value to reach 5--6, add complex enzyme, stir, enzymolysis 2-5 hour, get enzymolysis liquid; (3) enzymolysis liquid is filtered, remove impurity such as zymoprotein, get the enzymolysis clear liquid; (4) the enzymolysis clear liquid sent in the inspissator concentrated, concentrate; (5) concentrate squeezed into carried out spray-drying in the spray dryer, the solid shell oligosaccharides; (6) the solid shell oligosaccharides is mixed with the Chinese medicine pulverizing, get additive.
5, method according to claim 4 is characterized in that, this complex enzyme is made up of protease, elastoser, amylase, lipase, lysozyme, and its percentage by weight is 25-35%: 5-10%: 5-10%: 25-35%: 15-25%.Complex enzyme and chitosan oligosaccharide weight ratio are 5%-10%.
6, method according to claim 4 is characterized in that, acid solution can be selected from lactic acid, acetic acid, hydrochloric acid etc., acid concentration 0.5%-2%.
7, the purposes of the described product of claim 1 is characterized in that, product can be used for multiple poultry, fowl, aquatic livestocks such as ox, sheep, pig, chicken, duck, goose, fish, shrimp.
CNA200310105427XA 2003-10-23 2003-10-23 Feed additive containing chitin oligose and its prepn and use CN1608487A (en)

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102370056A (en) * 2010-08-24 2012-03-14 北京英惠尔生物技术有限公司 Method for preparing chitooligosaccharides used for feed by enzymatic hydrolysis
CN102423015A (en) * 2011-11-29 2012-04-25 天津中裕维特生物科技有限公司 Special synbioticmbiotic for broiler
CN102550845A (en) * 2010-12-17 2012-07-11 大连中科格莱克生物科技有限公司 Low-cholesterol egg
CN102934733A (en) * 2012-10-18 2013-02-20 肥西县皖高白鹅养殖农民专业合作社 Goose feed
CN103053862A (en) * 2013-02-02 2013-04-24 曾龙 Chinese herb composite premix for laying hens
CN103705533A (en) * 2012-09-29 2014-04-09 洛阳惠中兽药有限公司 Pharmaceutical composition for preventing poultry infectious diseases, preparation method thereof, and applications thereof
CN103815206A (en) * 2014-03-14 2014-05-28 仙桃市仙发饲料有限公司 Premixed fodder for 1% aquatic products and preparation method of premixed fodder
CN103875932A (en) * 2012-12-21 2014-06-25 中国科学院大连化学物理研究所 Functional glycoconjugate for improving sow breeding performance
CN105146099A (en) * 2015-10-29 2015-12-16 崔永进 Poultry feed additive for replacing antibiotics and promoting growth
CN105248979A (en) * 2015-11-20 2016-01-20 严建泗 Feed additive for meat geese and preparation method thereof
CN105961922A (en) * 2016-06-15 2016-09-28 广西大学 Feed for regulating and controlling sea water acidification stress of fishes
CN106107029A (en) * 2016-06-21 2016-11-16 宁波东瑭生物科技有限公司 A kind of biological Chinese medicine compound preparation with degraded antibiotics of animal residual and preparation method thereof
CN106333070A (en) * 2016-08-24 2017-01-18 蒋丽红 Total mixed ration used for fattening period of cattle and preparation method thereof
CN106755207A (en) * 2016-12-23 2017-05-31 浙江海洋大学 A kind of method of shitosan high solids content enzymolysis generation narrow molecular-weight activity chitosan oligosaccharide
CN106922988A (en) * 2017-03-29 2017-07-07 内蒙古河套农牧业技术研究院 A kind of Fattening Sheep mixed feed for reducing fat deposition

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102370056A (en) * 2010-08-24 2012-03-14 北京英惠尔生物技术有限公司 Method for preparing chitooligosaccharides used for feed by enzymatic hydrolysis
CN102370056B (en) * 2010-08-24 2013-09-11 北京英惠尔生物技术有限公司 Method for preparing chitooligosaccharides used for feed by enzymatic hydrolysis
CN102550845A (en) * 2010-12-17 2012-07-11 大连中科格莱克生物科技有限公司 Low-cholesterol egg
CN102550845B (en) * 2010-12-17 2014-01-01 大连中科格莱克生物科技有限公司 Low-cholesterol egg
CN102423015A (en) * 2011-11-29 2012-04-25 天津中裕维特生物科技有限公司 Special synbioticmbiotic for broiler
CN103705533B (en) * 2012-09-29 2016-12-21 洛阳惠中兽药有限公司 Pharmaceutical composition of preventing and treating poultry infectious disease and its preparation method and application
CN103705533A (en) * 2012-09-29 2014-04-09 洛阳惠中兽药有限公司 Pharmaceutical composition for preventing poultry infectious diseases, preparation method thereof, and applications thereof
CN102934733A (en) * 2012-10-18 2013-02-20 肥西县皖高白鹅养殖农民专业合作社 Goose feed
CN103875932A (en) * 2012-12-21 2014-06-25 中国科学院大连化学物理研究所 Functional glycoconjugate for improving sow breeding performance
CN103053862A (en) * 2013-02-02 2013-04-24 曾龙 Chinese herb composite premix for laying hens
CN103815206B (en) * 2014-03-14 2015-08-26 仙桃市仙发饲料有限公司 A kind of 1% aquatic products premixed feed and preparation method thereof
CN103815206A (en) * 2014-03-14 2014-05-28 仙桃市仙发饲料有限公司 Premixed fodder for 1% aquatic products and preparation method of premixed fodder
CN105146099A (en) * 2015-10-29 2015-12-16 崔永进 Poultry feed additive for replacing antibiotics and promoting growth
CN105248979A (en) * 2015-11-20 2016-01-20 严建泗 Feed additive for meat geese and preparation method thereof
CN105961922A (en) * 2016-06-15 2016-09-28 广西大学 Feed for regulating and controlling sea water acidification stress of fishes
CN106107029A (en) * 2016-06-21 2016-11-16 宁波东瑭生物科技有限公司 A kind of biological Chinese medicine compound preparation with degraded antibiotics of animal residual and preparation method thereof
CN106333070A (en) * 2016-08-24 2017-01-18 蒋丽红 Total mixed ration used for fattening period of cattle and preparation method thereof
CN106755207A (en) * 2016-12-23 2017-05-31 浙江海洋大学 A kind of method of shitosan high solids content enzymolysis generation narrow molecular-weight activity chitosan oligosaccharide
CN106922988A (en) * 2017-03-29 2017-07-07 内蒙古河套农牧业技术研究院 A kind of Fattening Sheep mixed feed for reducing fat deposition

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