CN108813126A - A kind of pseudosciaena crocea larvae composite immune reinforcing agent and its preparation method and application - Google Patents
A kind of pseudosciaena crocea larvae composite immune reinforcing agent and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to feeding additive aquatic animals more particularly to a kind of pseudosciaena crocea larvae composite immune reinforcing agent and its preparation method and application, by weight, including following components：8~12 parts of Radix Astragali, 10~16 parts of garlic powder, 8~15 parts of Poria cocos, 6~12 parts of fructus lycii, 6~10 parts of radix bupleuri, 8~16 parts of Bifidobacterium, 8~16 parts of biodiasmin, 5~10 parts of manna oligosacchride, 5~10 parts of chitosan oligosaccharide, 10~15 parts of vitamin C, 5~10 parts of vitamin E.Composite immune reinforcing agent of the invention has both the compound raising immunization of panimmunity reinforcing agent, can be used as feed addictive and uses in pseudosciaena crocea larvae basal feed, can replace antibiotic completely；Usage amount is low, and the composite immune reinforcing agent of addition 0.2~0.4% can effectively improve the immunity of organisms of pseudosciaena crocea larvae in feed, improves survival rate of fish fry, and have no adverse effects to the palatability of feed；Safety and environmental protection.
Description
Technical field
The present invention relates to feeding additive aquatic animal more particularly to a kind of pseudosciaena crocea larvae composite immune reinforcing agent and its
Preparation method and application.
Background technique
Larimichthys crocea (Larimichthys crocea) belongs to Perciformes (Perciformes), Sciaenidae
(Sciaenidae), yellow croaker category (Larimichthys) is the distinctive marine fish in China to warm up warm nature near sea fishes,
It is mainly distributed on China southeast sea area.Its fine and tender taste, delicious flavour is full of nutrition, is loved by consumers.
In the past 30 years, due to overfishing, cause rheum officinale fish resource increasingly depleted.To meet market to the need of Larimichthys crocea
It wants, from the eighties in last century, artificial breeding is carried out to it and nursery succeeds, has formed industrialization.Currently, Larimichthys crocea
Biological feed (such as wheel animalcule, artemia and Copepods etc.) is relied primarily in nursery production.As cultivation scale constantly expands, seed
Demand increase year by year, so as to cause biological feed need increase.However, since biological feed is expensive, quality and production
Measure unstable and the reasons such as pathogenic microorganism may be carried, have become limitation Larimichthys crocea nursery enlarged reproduction it is crucial because
Element.
With the expansion of Larimichthys crocea nursery scale, the demand to pseudosciaena crocea larvae feed is growing day by day.But it is domestic right
The research of Pseudosciaena crocea Fry opening material is also insufficient, and feed development more falls behind.
Currently, in Larimichthys crocea nursery production, survival rate of fish fry usually only 30~50%, and the influence vulnerable to disease, it educates
Seedling success rate is also possible to further to be affected.The survival rate for how improving Pseudosciaena crocea Fry, which becomes, develops pseudosciaena crocea larvae
The bottleneck of feed applications.
For improve aquatic livestock Anti-bacterium ability, it has been developed that it is a variety of improve aquatic livestock immunocompetence addition
Agent, including Chinese herbal and crude drugs preparations, probiotics, prebiotics, polysaccharide preparation, oligosaccharide kind preparation and vitamins additive etc..Medium-height grass
Medicine preparation, probiotics and prebiotics meet the needs of green aquatic products aquaculture development, eliminate people and feed out aquatic products to it
Safety worries, but curative effect is slower；The curative effects such as polysaccharide preparation such as astragalus polyose, beta-1,3-dextran are not significant.Therefore, have
Necessity develops a kind of specific complex immunopotentiator that can be improved pseudosciaena crocea larvae survival rate, digested and assimilated.
Summary of the invention
Inventor is the study found that when being used alone a certain immunity additive, the raising to pseudosciaena crocea larvae survival rate
It is unsatisfactory, and after inventor uses the reasonable compatibility use of panimmunity reinforcing agent, to the survival rate of fish fry of pseudosciaena crocea larvae
Improvement effect is significant.
The present invention provides a kind of pseudosciaena crocea larvae composite immune reinforcing agent, can significantly improve the fry of Larimichthys crocea at
Motility rate improves culture efficiency.
The present invention reaches above-mentioned purpose by following scheme：
In a first aspect, providing a kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, including with the following group
Point：8~12 parts of Radix Astragali, 10~16 parts of garlic powder, 8~15 parts of Poria cocos, 6~12 parts of fructus lycii, 6~10 parts of radix bupleuri, Bifidobacterium 8~
16 parts, 8~16 parts of biodiasmin, 5~10 parts of manna oligosacchride, 5~10 parts of chitosan oligosaccharide, 10~15 parts of vitamin C, vitamin E
5~10 parts.
Preferably, a kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, including following components：Radix Astragali
12 parts, 10 parts of garlic powder, 8 parts of Poria cocos, 6 parts of fructus lycii, 10 parts of radix bupleuri, 10 parts of Bifidobacterium, 8 parts of biodiasmin, manna oligosacchride
10 parts, 6 parts of chitosan oligosaccharide, 15 parts of vitamin C, 5 parts of vitamin E.
Preferably, a kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, including following components：Radix Astragali
10 parts, 12 parts of garlic powder, 9 parts of Poria cocos, 8 parts of fructus lycii, 8 parts of radix bupleuri, 12 parts of Bifidobacterium, 12 parts of biodiasmin, manna oligosacchride 5
Part, 8 parts of chitosan oligosaccharide, 10 parts of vitamin C, 6 parts of vitamin E.
Preferably, a kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, including following components：Radix Astragali 8
Part, 10 parts of garlic powder, 8 parts of Poria cocos, 12 parts of fructus lycii, 8 parts of radix bupleuri, 8 parts of Bifidobacterium, 16 parts of biodiasmin, manna oligosacchride 5
Part, 10 parts of chitosan oligosaccharide, 10 parts of vitamin C, 5 parts of vitamin E.
Preferably, a kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, including following components：Radix Astragali
10 parts, 12 parts of garlic powder, 15 parts of Poria cocos, 8 parts of fructus lycii, 8 parts of radix bupleuri, 8 parts of Bifidobacterium, 9 parts of biodiasmin, manna oligosacchride 5
Part, 5 parts of chitosan oligosaccharide, 10 parts of vitamin C, 10 parts of vitamin E.
Preferably, a kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, including following components：Radix Astragali
10 parts, 16 parts of garlic powder, 8 parts of Poria cocos, 7 parts of fructus lycii, 6 parts of radix bupleuri, 16 parts of Bifidobacterium, 8 parts of biodiasmin, manna oligosacchride 6
Part, 6 parts of chitosan oligosaccharide, 12 parts of vitamin C, 5 parts of vitamin E.
In second aspect, a kind of preparation method of above-mentioned pseudosciaena crocea larvae composite immune reinforcing agent is provided, by raw material
Component crushes, it is preferred to use after micronizer crushes, crosses sieve, such as the sieve of 200 mesh, obtains compound exempt from after mixing
Epidemic disease reinforcing agent.
Preferably, a kind of preparation method of above-mentioned pseudosciaena crocea larvae composite immune reinforcing agent, raw material components are used
After micronizer crushes, the sieve of 200 mesh is crossed, obtains composite immune reinforcing agent after mixing.
In the third aspect, a kind of application of above-mentioned pseudosciaena crocea larvae composite immune reinforcing agent is provided, including will be described
Composite immune reinforcing agent is added in pseudosciaena crocea larvae feed according to the weight percent of 0.2%-0.4% and feeds.
Above-mentioned pseudosciaena crocea larvae feed can use commercially available or conventional pseudosciaena crocea larvae basal feed.
It is preferred that following pseudosciaena crocea larvae feeds are used, in parts by weight, including following components：42 parts of white fish meal, the South Pole
15 parts of krill meal, 12 parts of squid powder, 8 parts of fish protein hydrolysate, 3 parts of yeast powder, 5 parts of pre-gelatinized starch, 1.5 parts of adhesive, fish oil 6
Part, 4 parts of phosphatide oil, 2 parts of Squid Oil, 1 part of vitamin mixtures, 0.5 part of mineral mixture.
It is further preferred that the squid powder is low temperature drying squid powder.
It is further preferred that the fish oil is refined fish oil.
Test confirms, the composite immune reinforcing agent of the invention of 0.2%-0.4% is added in pseudosciaena crocea larvae feed
Pseudosciaena crocea larvae nursery production is carried out, the survival rate and the speed of growth of pseudosciaena crocea larvae can be significantly improved.
In fourth aspect, a kind of application of above-mentioned pseudosciaena crocea larvae composite immune reinforcing agent, the immune increasing are provided
Strong agent is used to improve the survival rate and/or the speed of growth of pseudosciaena crocea larvae.
It in composite immune reinforcing agent of the invention, is compounded using various ingredients, the adjustable water of medicinal herb components
The endocrine system of animal is produced, stimulation liver pancreas generates growth factor, promotes the regeneration of liver Stem Cells, and strength repairs impaired liver pancreas
It is dirty and safe and free of toxic and side effects；Compound probiotic, which has, promotes intestinal flora balance, improves intestinal health and intestine immunity machine
The effect of energy；The interleukins matter in blood can be improved in oligosaccharide kind preparation；Vitamins additive such as vitamin C, dimension life
Plain E is biological anti-oxidant, can improve the anti-stress ability and immunity of aquatic livestock with the intracorporal free radical of removing machine.
The invention has the advantages that：
Composite immune reinforcing agent of the invention has both the compound raising immunization of panimmunity reinforcing agent, can be used as feed
Additive uses in pseudosciaena crocea larvae basal feed, can replace antibiotic completely；Its usage amount is low, and 0.2 is added in feed
~0.4% composite immune reinforcing agent can effectively improve the immunity of organisms of pseudosciaena crocea larvae, improve survival rate of fish fry, and
It has no adverse effects to the palatability of feed；Product is used for pollution-free, noresidue after Larimichthys crocea nursery, without any side effects and not
Good reaction, safety and environmental protection.
Specific embodiment
Below in conjunction with specific embodiment, invention is further explained.
Present invention be described in more detail combined with specific embodiments below, unless stated otherwise, reagent that the present invention uses,
Device and method are reagent, equipment and the conventional use of method of the art regular market purchase.The embodiment of the present invention is only made
For illustrative example, illustrate and not restrictive.
In the embodiment of the present invention and test example, the pseudosciaena crocea larvae basal feed formula used is following (with weight
Part meter)：42 parts of high-quality white fish meal, 15 parts of euphausia superba powder, 12 parts of low temperature drying squid powder, 8 parts of fish protein hydrolysate, yeast powder 3
Part, 5 parts of pre-gelatinized starch, 1.5 parts of adhesive, 6 parts of refined fish oil, 4 parts of phosphatide oil, 2 parts of Squid Oil, vitamin mixtures 1.0
Part, 0.5 part of mineral mixture.
Embodiment 1：
A kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, component is as follows：10 parts of Radix Astragali, garlic powder
12 parts, 9 parts of Poria cocos, 8 parts of fructus lycii, 8 parts of radix bupleuri, 12 parts of Bifidobacterium, 12 parts of biodiasmin, 5 parts of manna oligosacchride, chitosan oligosaccharide 8
Part, 10 parts of vitamin C, 6 parts of vitamin E.
The preparation of above-mentioned immunopotentiator, is crushed using micronizer, all crosses the sieve of 200 mesh, is uniformly mixed, is obtained
It arrives.
Embodiment 2
A kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, component is as follows：10 parts of Radix Astragali, garlic powder
16 parts, 8 parts of Poria cocos, 7 parts of fructus lycii, 6 parts of radix bupleuri, 16 parts of Bifidobacterium, 8 parts of biodiasmin, 6 parts of manna oligosacchride, chitosan oligosaccharide 6
Part, 12 parts of vitamin C, 5 parts of vitamin E.
The preparation of above-mentioned immunopotentiator, is crushed using micronizer, all crosses the sieve of 200 mesh, is uniformly mixed, is obtained
It arrives.
The application of above-mentioned immunopotentiator is added in pseudosciaena crocea larvae basal feed according to 0.2% ratio.
Embodiment 3
A kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, component is as follows：8 parts of Radix Astragali, garlic powder 10
Part, 8 parts of Poria cocos, 12 parts of fructus lycii, 8 parts of radix bupleuri, 8 parts of Bifidobacterium, 16 parts of biodiasmin, 5 parts of manna oligosacchride, chitosan oligosaccharide 10
Part, 10 parts of vitamin C, 5 parts of vitamin E.
The preparation of above-mentioned immunopotentiator, is crushed using micronizer, all crosses the sieve of 200 mesh, is uniformly mixed, is obtained
It arrives.
The application of above-mentioned immunopotentiator is added in pseudosciaena crocea larvae basal feed according to 0.2% ratio.
Embodiment 4
A kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, component is as follows：12 parts of Radix Astragali, garlic powder
10 parts, 8 parts of Poria cocos, 6 parts of fructus lycii, 10 parts of radix bupleuri, 10 parts of Bifidobacterium, 8 parts of biodiasmin, 10 parts of manna oligosacchride, chitosan oligosaccharide 6
Part, 15 parts of vitamin C, 5 parts of vitamin E.
The preparation of above-mentioned immunopotentiator, is crushed using micronizer, all crosses the sieve of 200 mesh, is uniformly mixed, is obtained
It arrives.
The application of above-mentioned immunopotentiator is added in pseudosciaena crocea larvae basal feed according to 0.2% ratio.
Embodiment 5
A kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, component is as follows：10 parts of Radix Astragali, garlic powder
12 parts, 15 parts of Poria cocos, 8 parts of fructus lycii, 8 parts of radix bupleuri, 8 parts of Bifidobacterium, 9 parts of biodiasmin, 5 parts of manna oligosacchride, chitosan oligosaccharide 5
Part, 10 parts of vitamin C, 10 parts of vitamin E.
The preparation of above-mentioned immunopotentiator, is crushed using micronizer, all crosses the sieve of 200 mesh, is uniformly mixed, is obtained
It arrives.
The application of above-mentioned immunopotentiator is added in pseudosciaena crocea larvae basal feed according to 0.2% ratio.
Embodiment 6
A kind of pseudosciaena crocea larvae composite immune reinforcing agent, by weight, component is as follows：10 parts of Radix Astragali, garlic powder
12 parts, 10 parts of Poria cocos, 7 parts of fructus lycii, 8 parts of radix bupleuri, 12 parts of Bifidobacterium, 10 parts of biodiasmin, 6 parts of manna oligosacchride, chitosan oligosaccharide
10 parts, 10 parts of vitamin C, 5 parts of vitamin E.
The preparation of above-mentioned immunopotentiator, is crushed using micronizer, all crosses the sieve of 200 mesh, is uniformly mixed, is obtained
It arrives.
The application of above-mentioned immunopotentiator is added in pseudosciaena crocea larvae basal feed, preparation according to 0.2% ratio
Experiment feed containing composite immune reinforcing agent of the present invention (crude protein content 54%, crude fat content 16%) out.
Test example 1
Using the composite immune reinforcing agent of embodiment 1 as raw material, gradient is added in pseudosciaena crocea larvae basal feed,
Addition gradient is respectively 0.0 (as a control group), 0.2,0.4,0.6 and 0.8%, is prepared containing composite immune reinforcing agent of the present invention
Experiment feed (crude protein content 54%, crude fat content 16%).
The above-mentioned five groups experiment feeds containing composite immune reinforcing agent of the invention are used for pseudosciaena crocea larvae culture experiment,
Process is as follows：Experiment floods to produce in Co., Ltd's experimental system in Bay, Ningde, Fujian Province richness to be carried out.Select 17 age in days pseudosciaena crocea larvaes
(0.014 ± 0.001g) is used as experimental animal, compares experiment, 4 repetitions of every kind of feed, each 8000 tail fish of repetition, often
It feeds feed by weight 8%~10%, feeds daily 6 times, and experiment carries out 28 days altogether, and the results are shown in Table 1.
1 pseudosciaena crocea larvae growing state contrast table of table
Table 1 the experimental results showed that, in feed addition including embodiment 1 including complex immunity provided by the present invention increasing
Strong agent significantly improves the long survival rate of pseudosciaena crocea larvae, terminal body, terminal weight and specific growth rate, and the present invention is compound to be exempted from
The broiler diets of epidemic disease reinforcing agent are 0.2~0.4%.All composite immune reinforcing agents of the invention have similar effect.
Test example 2
Test sets 5 processing groups altogether, and each processing group feeds a kind of test feed, and the setting of each group is as follows：
A：Basal control group (does not add the basal feed of the above-mentioned formula production of composite immune reinforcing agent)；B：Embodiment 2
Group (additive of+0.2% embodiment of the present invention 2 of basal feed production)；C：3 groups of embodiment (+0.2% present invention of basal feed
The additive that embodiment 3 produces)；D：4 groups of embodiment (additive of+0.2% embodiment of the present invention 4 of basal feed production)；E：
5 groups of embodiment (additive of+0.2% embodiment of the present invention 5 of basal feed production).
Experiment floods to produce in Co., Ltd's experimental system in Bay, Ningde, Fujian Province richness to be carried out.Select 18 age in days pseudosciaena crocea larvaes
(0.015 ± 0.001g) is used as experimental animal, compares experiment, 4 repetitions of every kind of feed, each 10000 tail fish of repetition, often
It feeds feed by weight 8%~10%, feeds daily 6 times, and experiment carries out 30 days altogether, and the results are shown in Table 2.
2 pseudosciaena crocea larvae growing state contrast table of table
Table 2 the experimental results showed that, compared with the control group, in feed add the embodiment of the present invention 2~5 complex immunity increase
Strong agent significantly improves the long survival rate of pseudosciaena crocea larvae, terminal body, terminal weight and specific growth rate, illustrates of the invention
Composite immune reinforcing agent can be improved the survival rate and the speed of growth of pseudosciaena crocea larvae.
Test example 3：
The experiment feed of composite immune reinforcing agent containing the embodiment of the present invention 6 is used for pseudosciaena crocea larvae nursery production.
Basal control group use without containing immunopotentiator basal feed, 6 groups of the embodiment of the present invention using contain 0.2% embodiment 6
Immunopotentiator basal feed.Experimentation is as follows：Experiment Bay, Ningde, Fujian Province richness flood produce Co., Ltd's nursery pond in into
Row.16 age in days pseudosciaena crocea larvaes (0.013 ± 0.001g) are selected as experimental animal, compare experiment, every kind 3, feed
It repeats, each 1,000,000 tail fish of repetition feeds feed by weight 8%~10% daily, feeds daily 8 times, and experiment carries out 28 altogether
It, the results are shown in Table 3.
3 pseudosciaena crocea larvae growing state contrast table of table
Table 3 the experimental results showed that, the composite immune reinforcing agent that 0.2% embodiment of the present invention 6 is added in feed significantly mentions
The high survival rate of pseudosciaena crocea larvae, terminal body be long, terminal weight and specific growth rate, improves the nursery effect of Larimichthys crocea
Rate.Other immunopotentiators of the invention have similar technical effect.
Test example 4：
Test sets 3 processing groups altogether, and each processing group feeds a kind of test feed, and the setting of each group is as follows：
Immunopotentiator A group：+ 0.2% composite immune reinforcing agent A of basal feed；Immunopotentiator B group：Basal feed+
0.2% composite immune reinforcing agent B；6 groups of the present embodiment：The additive of+0.2% embodiment of the present invention 6 of basal feed production.
Composite immune reinforcing agent A, component are following (by weight)：12 parts of Radix Astragali, 14 parts of garlic powder, 12 parts of Poria cocos, Chinese holly
10 parts of Qi, 8 parts of radix bupleuri, 16 parts of Bifidobacterium, 16 parts of biodiasmin, 12 parts of brewer's yeast.
Composite immune reinforcing agent B, component are following (by weight)：20 parts of brewer's yeast, 18 parts of fructooligosaccharide, manna oligosacchride
16 parts, 16 parts of chitosan oligosaccharide, 18 parts of vitamin C, 12 parts of vitamin E.
Experiment floods to produce in Co., Ltd's experimental system in Bay, Ningde, Fujian Province richness to be carried out.Select 17 age in days pseudosciaena crocea larvaes
(0.014 ± 0.001g) is used as experimental animal, compares experiment, 4 repetitions of every kind of feed, each 10000 tail fish of repetition, often
It feeds feed by weight 8%~10%, feeds daily 6 times, and experiment carries out 28 days altogether, and the results are shown in Table 4.
4 pseudosciaena crocea larvae growing state contrast table of table
Table 4 the experimental results showed that, the survival rates of 6 groups of pseudosciaena crocea larvaes of the embodiment of the present invention, terminal body be long, terminal body
Weight and specific growth rate are significantly higher than immunopotentiator A group and immunopotentiator B group, illustrate composite immune reinforcing agent of the invention
Immunoenhancement result be significantly better than composite immune reinforcing agent A and immunopotentiator B, the group of composite immune reinforcing agent of the invention
Dividing has cooperative synergism effect.
The above, preferable specific embodiment only of the invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (10)
1. a kind of pseudosciaena crocea larvae composite immune reinforcing agent, which is characterized in that by weight, including following components：It is yellow
8~12 parts of stilbene, 10~16 parts of garlic powder, 8~15 parts of Poria cocos, 6~12 parts of fructus lycii, 6~10 parts of radix bupleuri, 8~16 parts of Bifidobacterium,
8~16 parts of biodiasmin, 5~10 parts of manna oligosacchride, 5~10 parts of chitosan oligosaccharide, 10~15 parts of vitamin C, vitamin E 5~
10 parts.
2. a kind of pseudosciaena crocea larvae composite immune reinforcing agent according to claim 1, which is characterized in that by weight
Meter, including following components：12 parts of Radix Astragali, 8 parts of Poria cocos, 6 parts of fructus lycii, 10 parts of radix bupleuri, 10 parts of Bifidobacterium, is lived at 10 parts of garlic powder
Property 8 parts of lactic acid bacteria, 10 parts of manna oligosacchride, 6 parts of chitosan oligosaccharide, 15 parts of vitamin C, 5 parts of vitamin E.
3. a kind of pseudosciaena crocea larvae composite immune reinforcing agent according to claim 1, which is characterized in that by weight
Meter, including following components：10 parts of Radix Astragali, 12 parts of garlic powder, 9 parts of Poria cocos, 8 parts of fructus lycii, 8 parts of radix bupleuri, 12 parts of Bifidobacterium, activity
12 parts of lactic acid bacteria, 5 parts of manna oligosacchride, 8 parts of chitosan oligosaccharide, 10 parts of vitamin C, 6 parts of vitamin E.
4. a kind of pseudosciaena crocea larvae composite immune reinforcing agent according to claim 1, which is characterized in that by weight
Meter, including following components：8 parts of Radix Astragali, 10 parts of garlic powder, 8 parts of Poria cocos, 12 parts of fructus lycii, 8 parts of radix bupleuri, 8 parts of Bifidobacterium, activity
16 parts of lactic acid bacteria, 5 parts of manna oligosacchride, 10 parts of chitosan oligosaccharide, 10 parts of vitamin C, 5 parts of vitamin E.
5. a kind of pseudosciaena crocea larvae composite immune reinforcing agent according to claim 1, which is characterized in that by weight
Meter, including following components：10 parts of Radix Astragali, 12 parts of garlic powder, 15 parts of Poria cocos, 8 parts of fructus lycii, 8 parts of radix bupleuri, 8 parts of Bifidobacterium, activity
9 parts of lactic acid bacteria, 5 parts of manna oligosacchride, 5 parts of chitosan oligosaccharide, 10 parts of vitamin C, 10 parts of vitamin E.
6. a kind of pseudosciaena crocea larvae composite immune reinforcing agent according to claim 1, which is characterized in that by weight
Meter, including following components：10 parts of Radix Astragali, 16 parts of garlic powder, 8 parts of Poria cocos, 7 parts of fructus lycii, 6 parts of radix bupleuri, 16 parts of Bifidobacterium, activity
8 parts of lactic acid bacteria, 6 parts of manna oligosacchride, 6 parts of chitosan oligosaccharide, 12 parts of vitamin C, 5 parts of vitamin E.
7. the preparation method of any pseudosciaena crocea larvae composite immune reinforcing agent, special in a kind of claim 1 to 6
Sign is, raw material components are crushed, and crosses sieve, obtains composite immune reinforcing agent after mixing.
8. preparation method according to claim 7, which is characterized in that after raw material components are crushed using micronizer,
The sieve for crossing 200 mesh, obtains composite immune reinforcing agent after mixing.
9. the application of any pseudosciaena crocea larvae composite immune reinforcing agent in a kind of claim 1 to 6, including by institute
It states composite immune reinforcing agent and is added in pseudosciaena crocea larvae feed according to the weight percent of 0.2%-0.4% and fed；It is preferred that
, the pseudosciaena crocea larvae feed, in parts by weight, including following components：42 parts of white fish meal, 15 parts of euphausia superba powder, squid
12 parts of fish meal, 8 parts of fish protein hydrolysate, 3 parts of yeast powder, 5 parts of pre-gelatinized starch, 1.5 parts of adhesive, 6 parts of fish oil, 4 parts of phosphatide oil,
2 parts of Squid Oil, 1 part of vitamin mixtures, 0.5 part of mineral mixture.
10. the application of any pseudosciaena crocea larvae composite immune reinforcing agent, described to exempt from a kind of claim 1 to 6
Epidemic disease reinforcing agent is used to improve the survival rate and/or the speed of growth of pseudosciaena crocea larvae.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109775861A (en) * | 2019-01-15 | 2019-05-21 | 无锡华诺威动物保健品有限公司 | A kind of crab pond culture water body removing toxic substances Antistress agent and preparation method thereof |
| CN110896904A (en) * | 2019-12-27 | 2020-03-24 | 湖北省小龙虾产业技术研究院有限公司 | Method for improving shrimp fry transportation survival rate |
| CN111743055A (en) * | 2020-08-04 | 2020-10-09 | 广东海大集团股份有限公司 | Composite immunopotentiator for turbot and preparation method and application thereof |
| CN112471356A (en) * | 2020-12-03 | 2021-03-12 | 珠海容川饲料有限公司 | Compound feed capable of improving growth speed and immunity of raw fish and application thereof |
| CN114831227A (en) * | 2022-03-29 | 2022-08-02 | 诺立(上海)生物工程有限公司 | Composite peptide nutrition health-care conditioner for conditioning feed intake and immune function of aquatic animals |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609898A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of composite immunopotentiator for pseudosciaena crocea |
| CN107801867A (en) * | 2017-09-27 | 2018-03-16 | 浙江海洋大学 | A kind of large yellow croaker composite immune reinforcing agent and preparation method thereof |
-
2018
- 2018-07-06 CN CN201810738888.7A patent/CN108813126A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609898A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of composite immunopotentiator for pseudosciaena crocea |
| CN107801867A (en) * | 2017-09-27 | 2018-03-16 | 浙江海洋大学 | A kind of large yellow croaker composite immune reinforcing agent and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109775861A (en) * | 2019-01-15 | 2019-05-21 | 无锡华诺威动物保健品有限公司 | A kind of crab pond culture water body removing toxic substances Antistress agent and preparation method thereof |
| CN110896904A (en) * | 2019-12-27 | 2020-03-24 | 湖北省小龙虾产业技术研究院有限公司 | Method for improving shrimp fry transportation survival rate |
| CN111743055A (en) * | 2020-08-04 | 2020-10-09 | 广东海大集团股份有限公司 | Composite immunopotentiator for turbot and preparation method and application thereof |
| CN112471356A (en) * | 2020-12-03 | 2021-03-12 | 珠海容川饲料有限公司 | Compound feed capable of improving growth speed and immunity of raw fish and application thereof |
| CN114831227A (en) * | 2022-03-29 | 2022-08-02 | 诺立(上海)生物工程有限公司 | Composite peptide nutrition health-care conditioner for conditioning feed intake and immune function of aquatic animals |
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Application publication date: 20181116 |