CN104322948B - A kind of Chinese herbal feed additive and the application in mixed feed thereof - Google Patents

A kind of Chinese herbal feed additive and the application in mixed feed thereof Download PDF

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CN104322948B
CN104322948B CN201410561201.9A CN201410561201A CN104322948B CN 104322948 B CN104322948 B CN 104322948B CN 201410561201 A CN201410561201 A CN 201410561201A CN 104322948 B CN104322948 B CN 104322948B
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feed
chinese
root
chinese herbal
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CN104322948A (en
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苏记良
梁祖满
谢永东
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Guangdong Jingji Zhinong Technology Co.,Ltd.
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GUANGDONG KONDARL AGRO-PASTORAL TECHNOLOGY Co Ltd
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Abstract

The invention belongs to Feed Manufacturing manufacture field, relate to a kind of feed addictive and application thereof, be specifically related to a kind of Chinese herbal feed additive and the application in mixed feed thereof.The invention provides a kind of Novel Chinese herbal medicine feed additive, it is obtained by felwort, the Chinese bulbul, the Radix Astragali, golden cypress, hawthorn, red colouring agent for food, also used as a Chinese medicine, radish seed, the coptis, gynostemma pentaphylla, mango leaf, sweet osmanthus, pseudo-ginseng, the red sage root, the bighead atractylodes rhizome, fructus amomi, forestry greenstar root, forrest silkvine stem or root, Radix Codonopsis, japanese rose root, Chinese chestnut leaf, wilsonii, Radix Isatidis and Radix Glycyrrhizae; The effect that it has very strong raising animal disease resistant power, promotes growth of animal, improves survival rate, lowers diarrhea rate, thus has significant promotional value.

Description

A kind of Chinese herbal feed additive and the application in mixed feed thereof
Technical field
The invention belongs to Feed Manufacturing manufacture field, relate to a kind of feed addictive and application thereof, be specifically related to a kind of Chinese herbal feed additive and the application in mixed feed thereof.
Background technology
At present, Animal Husbandry in China is in the high-speed developing period, but also there are some problems while high speed development.Such as, Pig Industry produce in, due to breeding environment and cultural technique level delayed, make animal in flourish process be difficult to play its due hereditary potential.For playing the best production performance of sow, reducing sucking pig disease infection probability and improve sucking pig production performance, often adopt segregated early weaning technology.The digestive system of early weaned piglets is not yet grown perfect, be difficult to bear convert to cereal solid feed the nutrition brought by liquid breast milk stress; Sucking pig and dam are separated the psychological stress caused suddenly; And again build the environmental stress that the fight in group and grade status deterministic process between sucking pig individuality causes.Nutrition, psychology and environment are triple inevitably stress increase the rear sucking pig of wean to the sensitiveness of pathogenic microorganism potential in environment, sucking pig diarrhoea after causing weaning, feed intake decline, have the fidgets, aggressive reinforcement, growth retardation, degradation comprehensively stress under concurrent oedema and premunition, more easily there is archenteric flora imbalance, cause alimentary canal normal flora to be destroyed, the pathogenic strain such as Escherichia coli, Salmonella amount reproduction causes bacillary enteritis.Research shows, pig be the most easily stress animal species, and in intensive culture process, wean be again the most easily bring out sucking pig stress key link.Wean sucking pig stress often with the stagnation of growth of suckling pigs, produce cad pig when situation is serious, even cause sucking pig dead, cause tremendous economic to lose to Swine Production industry.
For a long time, antibiotic is at guarantee animal health, growth promoting effects, saving feed and play very important effect in increasing economic efficiency, but long-term a large amount of abuse of antibiotics can bring problems, as pathogen produces drug resistance, limit the performance of animal immune function, reduce immunity and the premunition of animal, suppress the growth of animal intestinal beneficial bacterium, destruction intestinal microecology balances, the growth of restriction animal.A lot of research shows, if the mankind eat the meat product of antibiotic excess residual for a long time, the mankind will be made to create antagonism and to give birth to the drug resistance of element, this not only can produce so-called ' resistance baby ', ' superbacteria ', and bring serious threat to human health existence, therefore, a lot of country forbids or limits the use of feeding antibiotic one after another in the world.European Union was from 1 day January in 2006, completely forbid and use antibiotic in animal feed, Japan from 2008 just forbid using in all antibiotic animal feeds, Korean government completely forbids from July 1st, 2011 and add antibiotic animal feed, and I am in state's feedstuff industry, in additive for feed for porkets, a large amount of antibiotic phenomenon of adding is still commonplace, cause that animal produces drug resistance or the resistance to the action of a drug, immunologic function reduces, resistance against diseases declines, have the problems such as antibiotic residue in pork, bring fear to people's life.In addition, because trace element plays very crucial effect, excessive interpolation phenomenon also ubiquity in feedstuff industry, especially copper and zinc in growth of animal and intestinal health control.And due to traditional inorganic microelement digestive utilization ratio low, excessive interpolation certainly leads to excessive emissions, and this not only causes waste, and give environment, the red tide of sea occurred in recent years and malicious grain phenomenon all follow animal husbandry relevant.Therefore, provide that a kind of diarrhea rate is low, digestive utilization ratio is high, feed intake and grow comparatively well suited under intensive culture pattern non-antibiotic class environment-protecting feed or environment-protecting feed additive seem particularly important.
In addition, although the high-quality feed raw material (as milk powder, SDPP, intestine membrane protein etc.) of piglet preweaning feed generally containing higher level in Swine Production, and excess adds antibiotic growth-promoting additive and the extra additive (as acidulant, enzyme, emulsifying agent etc.) supplementing regulating intestinal canal health.But ubiquity, and efficiency of feed utilization is not high, the dissatisfactory problem of suckling pig growth and development situation, this not only adds the production cost of raiser, and a large amount of nutritional labeling is discharged in environment, causes the huge wasting of resources and environmental protection pressure.The utilization rate improving feed has also become the demand of building a conservation-minded society.
CN103053854A discloses a kind of sucking pig Antistress agent and preparation method and sucking pig resisting stress water.Sucking pig Antistress agent, in every 500g Antistress agent, each composition is: vitamin A, vitamin E, corn alphalise starch, lactose, glucose, salt.By broken for salt fine powder, refinement sieve after salt mix with corn alphalise starch; Mix after successively adding VitAVitE; Mix after adding glucose, lactose.CN103125773A discloses a kind of composite feed additive for repairing sucking pig intestinal mucosa injury, and this feed addictive comprises: glutamine 0.1-0.2 part, butyric acid 0.01-0.1 part, yeast nucleic acid 0.01-0.1 part, threonine 0.02-0.2 part, origanum oil 0.03-0.3 part, chitosan oligosaccharide 0.03-0.3 part, active dry yeast 0.1-0.5 part, bacillus subtilis 0.1-0.5 part, vitamin 0.11-0.3 part, carrier 5-7.5 part.It is not good all to there is the long-term gaining effect of sucking pig in above-mentioned two pieces patent application, the defect that efficiency of feed utilization is not high.
Summary of the invention
Easily occur diarrhoea after sucking pig wean, the feed intake after causing sucking pig to be weaned is affected with growing, and causes tremendous economic to lose to cultivation industry.Existing piglet feed or additive for feed for porkets all well do not solve the problem, and a large amount of antibiotic of interpolation has simply had a strong impact on the upgrowth situation of sucking pig and the confidence of raiser.In order to overcome above-mentioned the deficiencies in the prior art, the invention provides a kind of Novel Chinese herbal medicine feed additive.
The present invention realizes above-mentioned technical purpose and is achieved through the following technical solutions:
A kind of Chinese herbal feed additive, according to components by weight percent meter, it is obtained by the Chinese drug preparation of following weight portion: felwort 10-16 part, Chinese bulbul 15-25 part, Radix Astragali 8-12 part, golden cypress 11-13 part, hawthorn 5-7 part, red colouring agent for food, also used as a Chinese medicine 10-16 part, radish seed 7-11 part, coptis 6-10 part, gynostemma pentaphylla 5-11 part, mango leaf 8-12 part, sweet osmanthus 7-11 part, pseudo-ginseng 16-22 part, red sage root 11-15 part, bighead atractylodes rhizome 6-8 part, fructus amomi 12-14 part, forestry greenstar root 13-17 part, forrest silkvine stem or root 14-17 part, Radix Codonopsis 11-14 part, japanese rose root 8-12 part, Chinese chestnut leaf 4-7 part, wilsonii 11-14 part, Radix Isatidis 9-13 part and Radix Glycyrrhizae 12-14 part.The present invention's Chinese herbal feed additive described above has very strong raising animal disease resistant power, promotes growth of animal, improves the effect that survival rate is high, lower diarrhea rate, thus has very large promotional value.
As Chinese herbal feed additive of the present invention one preferred embodiment, according to components by weight percent meter, it is obtained by the Chinese drug preparation of following weight portion: felwort 13 parts, the Chinese bulbul 20 parts, the Radix Astragali 10 parts, golden cypress 12 parts, hawthorn 6 parts, 13 parts, red colouring agent for food, also used as a Chinese medicine, radish seed 9 parts, the coptis 8 parts, gynostemma pentaphylla 8 parts, mango leaf 10 parts, sweet osmanthus 9 parts, pseudo-ginseng 19 parts, the red sage root 13 parts, the bighead atractylodes rhizome 7 parts, fructus amomi 13 parts, forestry greenstar root 15 parts, forrest silkvine stem or root 15 parts, Radix Codonopsis 13 parts, japanese rose root 10 parts, 6 parts, Chinese chestnut leaf, wilsonii 13 parts, Radix Isatidis 11 parts and 13 parts, Radix Glycyrrhizae.
The present invention also provides a kind of preparation method of said herbal medicine feed addictive, it comprises the steps: each medicinal material taking prescription weight portion, decoct 2-4 time in 60-90 DEG C after adding the water soaking at room temperature 10-60min of 7-10 times of medicinal material gross weight, each 60-120min, filter, merging filtrate, filtrate reduced in volume is to the medicinal extract of relative density 1.05-1.25, this relative density is the testing result at 60 DEG C, in medicinal extract, add ethanol to alcohol content is 60-90%, room temperature leaves standstill and gets supernatant after 24 hours, reclaims ethanol to concentrated, obtains Chinese medical concrete; Chinese medical concrete is dried to constant weight at 60-80 DEG C, crushes and screens until granularity is 30 order-80 orders, obtains described Chinese herbal feed additive.
The present invention also provides a kind of application of said herbal medicine feed addictive, i.e. the application of described Chinese herbal feed additive in mixed feed.Described Chinese herbal feed additive directly can add in solid form in feed or after being blunged by feed and adds feed addictive of the present invention again.Described mixed feed may be used for feeding of poultry or livestock, is preferably piglet and feeds.During actual use, the proportioning of feed addictive of the present invention and feed can adjust flexibly according to the content of main component contained in feed, preferred feed addictive: conventional feed is 1:250-1000, more preferably 1:500.That is add feed addictive 1-4kg of the present invention in conventional feed per ton, be preferably 2kg.By the impact of examples prove feed addictive of the present invention on sucking pig production performance and diarrhea rate, result shows intestinal growth and the health that feed addictive of the present invention significantly can improve wean sucking pig, sucking pig ablactation stress can be alleviated, effectively improve the rear feed intake of sucking pig wean and weightening finish.After corresponding to use embodiment 3, additive mixes in varing proportions with conventional feed, when the mixing ratio of feed addictive and conventional feed is 1:500, the improvement effect of the production performance to sucking pig of feed addictive of the present invention is more excellent, and its each stage feed intake for sucking pig, weightening finish compare other groups with control diarrhoea aspect more excellent.After using the feed addictive described in embodiment 1-embodiment 3 to mix with the ratio of 1:500 with conventional feed, adopt Chinese herbal feed additive described in embodiment 3 more excellent compared to the improvement effect of other Chinese herbal feed additives organized to the production performance of sucking pig, this is indicated as preferred embodiment, and the optimum mixture ratio example of described Chinese herbal feed additive and conventional feed is 1:500.
The invention has the advantages that:
(1) contain plurality of Chinese component in additive for feed for porkets of the present invention, and have product analysis guarantee value, the nutritional need of sucking pig can be met, alleviate ablactation stress, improve intestinal health, improve wean sucking pig daily gain and feed efficiency.
(2) feed addictive provided by the invention is applicable to the production of sucking pig perfect compound feed, and by the mixed feed of this production, comprehensive nutrition is balanced, can meet the nutritional need of sucking pig, and can strengthen sucking pig production performance.
(3) the invention provides not containing antibiotic in feed addictive, not producing any destruction to the enteron aisle of sucking pig, can not increase antibiotic resistance risk, and can reduce feeding cost, improve environmental sanitation, is environment-protecting feed additive.
Detailed description of the invention
Further describe the present invention below by way of detailed description of the invention, but the present invention is not limited only to following examples.Within the scope of the invention or not departing from content of the present invention, spirit and scope, Chinese medicine composition of the present invention is suitably improved, replaces the identical component of effect, will become apparent to those skilled in the art that they are all deemed to be included within scope of the present invention.
embodiment 1 Chinese herbal feed additive of the present invention
Prescription: felwort 100g, Chinese bulbul 150g, Radix Astragali 80g, golden cypress 110g, hawthorn 50g, red colouring agent for food, also used as a Chinese medicine 100g, radish seed 70g, coptis 60g, gynostemma pentaphylla 50g, mango leaf 80g, sweet osmanthus 70g, pseudo-ginseng 160g, red sage root 110g, bighead atractylodes rhizome 60g, fructus amomi 120g, forestry greenstar root 130g, forrest silkvine stem or root 140g, Radix Codonopsis 110g, japanese rose root 80g, Chinese chestnut leaf 40g, wilsonii 110g, Radix Isatidis 90g and Radix Glycyrrhizae 120g.
Preparation method: each medicinal material taking prescription weight, decoct 4 times in 60 DEG C after adding the water soaking at room temperature 10min of 7 times of medicinal material gross weight, each 60min, filters, merging filtrate, filtrate reduced in volume is to the medicinal extract of relative density 1.05, this relative density is the testing result at 60 DEG C, and in medicinal extract, add ethanol to alcohol content is 60%, and room temperature leaves standstill and gets supernatant after 24 hours, reclaim ethanol to concentrated, obtain Chinese medical concrete; Chinese medical concrete is dried to constant weight at 60 DEG C, crushes and screens until granularity is 60 orders, obtains described Chinese herbal feed additive.
embodiment 2 Chinese herbal feed additive of the present invention
Prescription: felwort 160g, Chinese bulbul 250g, Radix Astragali 120g, golden cypress 130g, hawthorn 70g, red colouring agent for food, also used as a Chinese medicine 160g, radish seed 110g, coptis 100g, gynostemma pentaphylla 110g, mango leaf 120g, sweet osmanthus 110g, pseudo-ginseng 220g, red sage root 150g, bighead atractylodes rhizome 80g, fructus amomi 140g, forestry greenstar root 170g, forrest silkvine stem or root 170g, Radix Codonopsis 140g, japanese rose root 120g, Chinese chestnut leaf 70g, wilsonii 140g, Radix Isatidis 130g and Radix Glycyrrhizae 140g.
Preparation method: each medicinal material taking prescription weight, decoct 2 times in 90 DEG C after adding the water soaking at room temperature 60min of 10 times of medicinal material gross weight, each 60min, filters, merging filtrate, filtrate reduced in volume is to the medicinal extract of relative density 1.25, this relative density is the testing result at 60 DEG C, and in medicinal extract, add ethanol to alcohol content is 90%, and room temperature leaves standstill and gets supernatant after 24 hours, reclaim ethanol to concentrated, obtain Chinese medical concrete; Chinese medical concrete is dried to constant weight at 80 DEG C, crushes and screens until granularity is 30 orders, obtains described Chinese herbal feed additive.
embodiment 3 Chinese herbal feed additive of the present invention
Prescription: felwort 130g, Chinese bulbul 200g, Radix Astragali 100g, golden cypress 120g, hawthorn 60g, red colouring agent for food, also used as a Chinese medicine 130g, radish seed 90g, coptis 80g, gynostemma pentaphylla 80g, mango leaf 100g, sweet osmanthus 90g, pseudo-ginseng 190g, red sage root 130g, bighead atractylodes rhizome 70g, fructus amomi 130g, forestry greenstar root 150g, forrest silkvine stem or root 150g, Radix Codonopsis 130g, japanese rose root 100g, Chinese chestnut leaf 60g, wilsonii 130g, Radix Isatidis 110g and Radix Glycyrrhizae 130g.
Preparation method: each medicinal material taking prescription weight, decoct 3 times in 60-90 DEG C after adding the water soaking at room temperature 30min of 8 times of medicinal material gross weight, each 90min, filters, merging filtrate, filtrate reduced in volume is to the medicinal extract of relative density 1.15, this relative density is the testing result at 60 DEG C, and in medicinal extract, add ethanol to alcohol content is 75%, and room temperature leaves standstill and gets supernatant after 24 hours, reclaim ethanol to concentrated, obtain Chinese medical concrete; Chinese medical concrete is dried to constant weight at 70 DEG C, crushes and screens until granularity is 80 orders, obtains described Chinese herbal feed additive.
embodiment 4: feed addictive different blended composition and division in a proportion of the present invention is on the impact of sucking pig production performance
1, experimental technique
With the feed addictive corresponding to embodiment 3 for research object, determine that feed addictive different blended composition and division in a proportion is on the impact of sucking pig production performance.Employing one factor factorial experiment with complete randomization design designs, and choose 128 rear sucking pigs of healthy wean, male and female half and half, is divided into 4 groups at random, often organizes 4 repetitions, each repetition 8 sucking pigs.Each group of following feed of feeding respectively:
Feed 1 group: embodiment 3 feed addictive mixes with conventional feed, the mixing ratio of feed addictive and conventional feed is 1:250;
Feed 2 groups: embodiment 3 feed addictive mixes with conventional feed, the mixing ratio of feed addictive and conventional feed is 1:500;
Feed 3 groups: embodiment 3 feed addictive mixes with conventional feed, the mixing ratio of feed addictive and conventional feed is 1:1000;
Control group: conventional feed;
Before test is raised 14 day age, carry out empty stomach and weigh, calculate weightening finish and feedstuff-meat ratio.Feedstuff-meat ratio is the ratio that feeding feed stuff gross weight and sucking pig increase weight.The component of the present embodiment conventional feed is constructed as follows: corn 40%, popcorn 20%, full-fat bean 10%, fish meal 5%, whey powder 5%, powered milk substitute 4%, fermented bean dregs 6%, dregs of beans 4%, premix 6%.
2, feeding and management:
Conveniently pig house is carried out disinfection, adopt high bed crack to raise to pig, make its free choice feeding, drinking-water.
3, sample collection and Testing index
The mensuration of weightening finish and feedstuff-meat ratio: carry out empty stomach in the 14th day morning of experimental period to pig and weigh, calculates pig weightening finish and feedstuff-meat ratio.
Feed intake and the diarrhea rate of sucking pig is often organized every day in record experimental period.
4, result of the test and discussion
Table 1 feed addictive different blended composition and division in a proportion is on the impact of 0-14 days production performances after sucking pig wean
Compare with control group, #p < 0.05, ##p < 0.01;
As can be seen from Table 1, adopt 1 group of-3 group sucking pig that feed addictive of the present invention is fed, the feed intake within the latter 14 day stage of wean, weightening finish and diarrhoea control rate are all significantly better than control group.This shows that feed addictive of the present invention significantly can improve intestinal growth and the health of wean sucking pig, can alleviate sucking pig ablactation stress, effectively improves the rear feed intake of sucking pig wean and weightening finish.Feed 1 group, feed 2 groups and compare with feeding 3 groups, the feed intake in the latter 14 day stage of the wean of feeding corresponding to 2 groups, weightening finish and diarrhoea control rate are better.This shows to adopt the feed addictive described in embodiment 3, and when adding proportion is 1:500, the improvement effect of the production performance to sucking pig of feed addictive of the present invention is more excellent.
embodiment 5 feed addictive of the present invention is on the impact of sucking pig production performance
This experiment adopts one factor factorial experiment with complete randomization design design, chooses 128 rear sucking pigs of healthy wean, is divided into 4 groups at random, male and female half and half, often organizes 4 repetitions, each repetition 8 sucking pigs.。Each group of following feed of feeding respectively:
Feed 1 group: embodiment 1 feed addictive mixes with conventional feed, the mixing ratio of feed addictive and feed is 1:500;
Feed 2 groups: embodiment 2 feed addictive mixes with conventional feed, the mixing ratio of feed addictive and feed is 1:500;
Feed 3 groups: embodiment 3 feed addictive mixes with conventional feed, the mixing ratio of feed addictive and feed is 1:500; Control group: conventional feed;
Described conventional feed, feeding and management and sample collection Testing index are identical with the embodiment of the present invention 4.
Table 2 feed addictive is on the impact of 0-14 days production performances after sucking pig wean
Compare with control group, #p < 0.05, ##p < 0.01;
As can be seen from Table 2, adopt 1 group of-3 group sucking pig that feed addictive of the present invention is fed, the feed intake within the latter 14 day stage of wean, weightening finish and diarrhoea control rate are all significantly better than control group.This shows that feed addictive of the present invention significantly can improve intestinal growth and the health of wean sucking pig, can alleviate sucking pig ablactation stress, effectively improves the rear feed intake of sucking pig wean and weightening finish.Feeding in 1 group of-3 group, feeding 3 groups, in each stage feed intake, weightening finish and control diarrhoea, compare other groups more excellent, is most preferred embodiment of the present invention.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (5)

1. a Chinese herbal feed additive, according to components by weight percent meter, it is obtained by the Chinese drug preparation of following weight portion: felwort 13 parts, the Chinese bulbul 20 parts, the Radix Astragali 10 parts, golden cypress 12 parts, hawthorn 6 parts, 13 parts, red colouring agent for food, also used as a Chinese medicine, radish seed 9 parts, the coptis 8 parts, gynostemma pentaphylla 8 parts, mango leaf 10 parts, sweet osmanthus 9 parts, pseudo-ginseng 19 parts, the red sage root 13 parts, the bighead atractylodes rhizome 7 parts, fructus amomi 13 parts, forestry greenstar root 15 parts, forrest silkvine stem or root 15 parts, Radix Codonopsis 13 parts, japanese rose root 10 parts, 6 parts, Chinese chestnut leaf, wilsonii 13 parts, Radix Isatidis 11 parts and 13 parts, Radix Glycyrrhizae.
2. prepare the method for Chinese herbal feed additive as claimed in claim 1 for one kind, it is characterized in that, it comprises the following steps: each medicinal material taking prescription weight portion, decoct 2-4 time in 60-90 DEG C after adding the water soaking at room temperature 10-60min of 7-10 times of medicinal material gross weight, each 60-120min, filter, merging filtrate, filtrate reduced in volume is to the medicinal extract of relative density 1.05-1.25, this relative density is the testing result at 60 DEG C, in medicinal extract, add ethanol to alcohol content is 60-90%, room temperature leaves standstill and gets supernatant after 24 hours, reclaim ethanol to concentrated, obtain Chinese medical concrete, Chinese medical concrete is dried to constant weight at 60-80 DEG C, crushes and screens until granularity is 30 order-80 orders, obtains described Chinese herbal feed additive.
3. Chinese herbal feed additive as claimed in claim 1 is preparing the application in feed, it is characterized in that, the feed postition of feed addictive directly adds in piglet feed or after being blunged by piglet feed in solid form and adds; The ratio of weight and number 1:500 of feed addictive and conventional feed.
4. apply as claimed in claim 3, it is characterized in that, described feed addictive can reduce sucking pig Post Weaning Diarrhea rate, improves the healthy immune level of sucking pig.
5. apply as claimed in claim 3, it is characterized in that, described feed addictive can improve the rear feed intake of sucking pig wean and weightening finish.
CN201410561201.9A 2014-10-21 2014-10-21 A kind of Chinese herbal feed additive and the application in mixed feed thereof Active CN104322948B (en)

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CN105231046A (en) * 2015-11-05 2016-01-13 农鑫高科(北京)科技开发有限公司 Feed preventing white scour of piglets and preparation method thereof
CN106509499A (en) * 2016-12-08 2017-03-22 西南大学 Feed additive capable of improving immunity of fancy carps and preparation method thereof
CN111227124A (en) * 2020-03-26 2020-06-05 重庆兽星饲料有限公司 Functional antibiotic-free feed additive for cattle and sheep
CN113208002A (en) * 2021-05-12 2021-08-06 鹤壁市金猪食品有限公司 Chinese herbal medicine additive for improving pork quality and using method thereof

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