The anti-of a kind of Penaeus Vannmei special use stress Chinese herbal composite feed additive
Technical field
The present invention relates to the feeding additive aquatic animal field, being specifically related to the anti-of a kind of Penaeus Vannmei special use stress Chinese herbal composite feed additive.
Background technology
Penaeus Vannmei (Penaeus Vannamei) is under the jurisdiction of Penaeidae, Penaeus, white shrimp subgenus, also referred to as Penaeus vannamei, Environment of Litopenaeus vannamei Low etc., be one of 3 large kinds that world today's shrimps in culture output is the highest (Penaeus monodon, Penaeus Vannmei and Chinese prawn).With domestic other cultured prawns, compare, have growth fast, adapt to that salinity range is wide, resistance against diseases strong, containing meat amount advantages of higher.Spreading all over rapidly the whole nation after within 1988, introducing China, has been at present the prawn kind that China's prawn culturing area is maximum, output is the highest." national fishery economic communique in 2010 " data show, 154.47 ten thousand tons of shrimp culture output in 2010 are 61.50 ten thousand tons of Penaeus Vannmeis wherein.
Penaeus Vannmei is very responsive to environmental change, these stimulations in various degree such as water temperature sudden change, water quality mutation, cultivation density are too high, scaring, feed nutrition are unbalanced easily make prawn in " stress " state, thereby the symptoms such as food ration descends, dysphoria, shelling difficulty appear, cause the poor growth situation that even death rate rises, the cultivation success rate is not high.Cause the serious consequences such as in the culture of Penaeus vannamei process, the death rate is high, success rate is low, low in economic efficiency.
Development along with the prawn industry, the high density intensive culture has become the current main aquaculture model of China's Penaeus Vannmei, stress for what solve in breeding process that environmental factor causes, raiser's solution commonly used is to use in a large number antibiotic, a large amount of antibiotic that use bring increasing negative effect to shrimp culture industry, are unfavorable for that the aquaculture sustainable development more is unfavorable for human health.Urgently develop the efficient Penaeus Vannmei Antistress agent of a kind of environmental protection.
But up to now, few about anti-research that stress aspect, few about the anti-research that stress aspect of prawn, existing shrimp crab Antistress agent lacks specific aim in the market, there is no the anti-stress feed addictive that Penaeus Vannmei uses and come out.
Application number is
200410075512.0chinese invention patent application a kind of prawn Antistress agent is disclosed, this Antistress agent mainly comprises CLA, beta glucan, vitamins, mineral salt etc.The described Antistress agent of this application is mainly to strengthen resistance against diseases by adding the vitamin mineral salt.
Application number is
200510094703.6chinese invention patent application one seed shrimp crab anti-stress health-care agent is disclosed, this health-care agent mainly comprises glucose, vitamin and Chinese herbal medicine, and this patent application is for improving immunity and the anti-stress ability of the shrimp crayfish culture animals such as river crab, swimming crab, Penaeus Vannmei, Macrobrachium rosenbergii.
Application number is
200810116768.Xchinese invention patent application a kind of anti-stress feed addictive is disclosed, this additive mainly comprises Chinese herbal medicine extract, vitamin, mineral salt etc., the described feed addictive of this patent application is particularly suited for strengthening anti-cold stress and heat stress ability.
Application number is
200910156923.5chinese invention patent application a kind of used for aquiculture water splashing type Antistress agent is disclosed, this additive mainly comprises glucose, amino acid, vitamin, Chinese medical extract etc., the described Antistress agent of this patent application improves the anti-oxidant and high-temperature resistance of aquiculture animal, improves survival rate and the disease-resistant rate of aquaculture organisms.
Application number is
201010207285.8chinese invention patent application a kind of anti-stress agent for aquatic animal is disclosed; this Antistress agent mainly comprises vitamin, carnitine, taurine; this patent application can be taken orally again in external application; not only there is anti-stress effect; improve grazing rate; promote growth, can improve organism immunity of aquatic livestock simultaneously.
Application number is
201110048026.Xchinese invention patent application disclose a kind of promote shrimp crab shell duricrust and diseases prevention anti-stress water splashing compound preparation, this compound formulation mainly comprises glucose, mineral salt, amino acid, vitamin, moulting hormone and Chinese herbal medicine etc., normal physiological function and the immunologic function of the fast quick-recovery grow shrimp of this patent application crab, play promote shell, the solid shell of replenishing the calcium, diseases prevention anti-stress effect.
Application number is
201110145031.2chinese invention patent application disclose and a kind ofly anti-stress add feed by humic acid, this formula mainly comprises humic acid, Chinese herbal medicine, vitamin, amino acid, mineral salt, complex enzyme formulation etc.This patent application humic acid adds the synergy that feed link is crossed each component, solves the excessive stress reaction of animal, can be applicable to the animal wean, animal hives off, adjusts and raise house and load while transporting.
Summary of the invention
The present invention is intended to according to motherland's traditional Chinese medicine theory in conjunction with modern aquatic livestock nutrient research technology and long term production experience, overcome the deficiency on prior art, a kind of raising Penaeus Vannmei anti-stress ability is provided, strengthen Penaeus Vannmei immunity, the Chinese herbal composite feed additive that the Penaeus Vannmei of raising cultivation survival rate is used.
The traditional Chinese herbal medicine of China has the advantages such as cost is low, easy to process, little without the resistance to the action of a drug and medicament residue, side effect, aboundresources, Chinese herb feed additive not only contains abundant nutriment, and the some of them active component can improve the feed palatability, improve the fishes and shrimps food ration, improve efficiency of feed utilization.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of Penaeus Vannmei with anti-stress Chinese herbal composite feed additive, comprise Chinese herbal medicine (fushen, spina date seed, Radix Angelicae Sinensis, the banksia rose, polygala root, Radix Codonopsis, rhizoma zingiberis) and vitamin (vitamin C, vitamin E, calcium pantothenate).
In above-mentioned feed addictive, by percentage to the quality, its component is 15 ~ 25% fushens, 15 ~ 25% spina date seeds, 10 ~ 15% Radix Angelicae Sinensis, 10 ~ 17% banksia rose, 5 ~ 8% polygala roots, 5 ~ 8% Radix Codonopsis, 4 ~ 6% rhizoma zingiberis, 4 ~ 5% vitamin Cs, 5 ~ 6% vitamin Es, 4 ~ 5% calcium pantothenates.
A kind of method of above-mentioned Penaeus Vannmei with anti-stress feed addictive that prepare, pulverize described raw material through micronizer, all by 200 purpose screen clothes, after mixing, obtain feed addictive of the present invention.
The anti-using method that stress Chinese herbal composite feed additive of above-mentioned raising Penaeus Vannmei, the percentage by weight according to 0.2 ~ 0.3% adds it in penaeus vannamei boone feed to, mixes and gets final product.
Compared with prior art, the present invention has following advantage:
(1) Chinese herbal medicine is the rarity in motherland's medical science, as feed addictive, uses and to compare with other feed addictives, has widely distributedly, and toxic and side effect is little, without hormone, is difficult for characteristics such as environments.Constituent of the present invention be take Chinese herbal medicine as main, the panimmunity that Chinese herbal medicine contains is regulated composition, can strengthen the Penaeus Vannmei anti-stress ability and improve immunity, can effectively alleviate Penaeus Vannmei because water temperature sudden change, water quality mutation, cultivation density are too high, scaring etc. cause stress, thereby can prevent the farming disease harms to occur, improve the cultivation success rate.
(2) to take the Chinese herbal medicines such as the sweet flat fushen of preferred nature and flavor, spina date seed, Radix Angelicae Sinensis, the banksia rose be primary raw material in the present invention, as additive, use, the good palatability of feed, ratio according to 0.2 ~ 0.3% in feed is added, not only can improve the Penaeus Vannmei anti-stress ability, can also obviously improve the Penaeus Vannmei food ration, improve efficiency of feed utilization.
(3) the present invention effectively improves the cultivation survival rate of Penaeus Vannmei, improves the output of Penaeus Vannmei, reduces aquaculture cost, adapts to Penaeus Vannmei large-scale cultivation needs.
(4) the present invention is usingd common Chinese herbal medicine as primary raw material, and China's Chinese herbal medicine resource is abundant, and raw material sources are easy.
(5) proportioning of the present invention is simple, operation is simple, with low cost, be suitable for promoting.
The specific embodiment
Further explain the present invention below in conjunction with embodiment, but embodiment does not limit in any form to the present invention.
embodiment 1
As a kind of preferred version, take fushen 20kg, spina date seed 15kg, Radix Angelicae Sinensis 15kg, banksia rose 17kg, polygala root 8kg, Radix Codonopsis 5kg, rhizoma zingiberis 5kg, vitamin C 5kg, vitamin E 5kg, calcium pantothenate 5kg, through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in litopenaeus vannamei compound feed.
embodiment 2
As a kind of preferred version, take fushen 25kg, spina date seed 18kg, Radix Angelicae Sinensis 14kg, banksia rose 10kg, polygala root 8kg, Radix Codonopsis 5kg, rhizoma zingiberis 5kg, vitamin C 5kg, vitamin E 5kg, calcium pantothenate 5kg, through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.3% after mixing is added in litopenaeus vannamei compound feed.
embodiment 3
As a kind of preferred version, take fushen 25kg, spina date seed 16kg, Radix Angelicae Sinensis 13kg, banksia rose 12kg, polygala root 7kg, Radix Codonopsis 7kg, rhizoma zingiberis 4kg, vitamin C 5kg, vitamin E 6kg, calcium pantothenate 5kg, through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in litopenaeus vannamei compound feed.
embodiment 4
As a kind of preferred version, take fushen 15kg, spina date seed 25kg, Radix Angelicae Sinensis 12kg, banksia rose 12kg, polygala root 8kg, Radix Codonopsis 8kg, rhizoma zingiberis 6kg, vitamin C 5kg, vitamin E 5kg, calcium pantothenate 4kg, through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in litopenaeus vannamei compound feed.
embodiment 5
As a kind of preferred version, take fushen 20kg, spina date seed 22kg, Radix Angelicae Sinensis 16kg, banksia rose 14kg, polygala root 5kg, Radix Codonopsis 5kg, rhizoma zingiberis 4kg, vitamin C 4kg, vitamin E 5kg, calcium pantothenate 5kg, through micronizer, pulverize, all, through 200 purpose screen clothes, the ratio according to 0.2% after mixing is added in litopenaeus vannamei compound feed.
Result of the test
Tested on May 13rd, 2011 to June 23 in cultivation base, East Sea Island, Zhanjiang City circulation, Penaeus Vannmei derives from Haixing County's agriculture of sea, Guangdong large group with batch thick shrimp seedling of hatching mark, select healthy, shrimp seedling of uniform size, support and be divided into 4 groups after 5 days temporarily, be respectively embodiment 1, embodiment 2, embodiment 3 and control group.Every group of 50 tails, establish 4 repetitions for every group.
Throw something and feed twice at 9:00 and 15:00 every day, and daily ration, feeding quantity did not remain and is as the criterion in two hours.Culture experiment continues 42 days, and the duration of test water temperature remains on 25~27 ℃, the 24h continuous charge.After off-test, measure the Penaeus Vannmei growth indexes, get blood system and carry out the analysis of salivary lysozyme from serum, result of the test is as table 1 and table 2.
Anti-stress Penaeus Vannmei body weight, rate of body weight gain, feed coefficient and survival rate the impacts Chinese herbal composite feed additive that table 1 Penaeus Vannmei is used
Processed group |
Control group |
Embodiment 1 |
Embodiment 2 |
Embodiment 3 |
Initial counterpoise (g) |
0.41±0.01 |
0.41±0.03 |
0.41±0.08 |
0.41±0.02 |
Whole last counterpoise (g) |
3.05±0.38a |
3.27±0.25b |
3.29±0.17bc |
3.31±0.33c |
Rate of body weight gain (%) |
643.52±37.59a |
725.38±46.47b |
720.52±38.59b |
728.11±66.67c |
Feed coefficient |
1.44±0.71a |
1.16±0.19b |
1.23±0.68c |
1.20±0.14c |
Survival rate (%) |
85.1a |
93.6b |
95.3b |
93.9b |
Table 2 Penaeus Vannmei is used anti-stress Chinese herbal composite feed additive on the impact of Penaeus Vannmei immunity of organisms
Processed group |
Control group |
Embodiment 1 |
Embodiment 2 |
Embodiment 3 |
Serum superoxide dismutases SOD(u/mg) |
298±33.69a |
471.2±56.92b |
500.84±69.17c |
486.19±23.65b |
Serum lysozyme UI(U/ml) |
0.96±0.79a |
1.68±0.21b |
2.78±0.44c |
2.55±0.48c |
Serum phenol oxidase PO(U/ml) |
4.2±0.26a |
11.31±1.24b |
11.69±0.37b |
9.09±0.25c |
As seen from the experiment, the present invention can significantly improve the Penaeus Vannmei anti-stress ability, strengthens the Penaeus Vannmei immunity of organisms, improves survival rate, improves efficiency of feed utilization, reduces feed coefficient.