CN106615728A - Preparation method of microbial preparation for calves - Google Patents

Preparation method of microbial preparation for calves Download PDF

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Publication number
CN106615728A
CN106615728A CN201611024174.7A CN201611024174A CN106615728A CN 106615728 A CN106615728 A CN 106615728A CN 201611024174 A CN201611024174 A CN 201611024174A CN 106615728 A CN106615728 A CN 106615728A
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CN
China
Prior art keywords
calf
preparation
vitamin
microorganism formulation
yeast
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CN201611024174.7A
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Chinese (zh)
Inventor
杨远澄
周贵金
谭春萍
杨远广
陆海龙
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Guangxi Shanshui cattle husbandry Co., Ltd.
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Guangxi Shanshui Agriculture Co Ltd
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Priority to CN201611024174.7A priority Critical patent/CN106615728A/en
Publication of CN106615728A publication Critical patent/CN106615728A/en
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention discloses a preparation method of a microbial preparation for calves. According to the method, the microbial preparation is prepared by extruding and pelletizing a traditional Chinese medicine extract solution, a compound microbial agent, vitamin C, vitamin E, bran and bean flour. The microbial preparation has the effects of improving the intestinal functions of calves, relieving the diseases of diarrhea and the like and promoting growth of the calves; the resistance of the calves can be improved by the traditional Chinese medicine solution, the vitamin C and the vitamin E; the morbidity is reduced. The used yeast is a Se-enriched yeast, and the calves can become se-enriched products after being bred for a long period of time, so that the additional value is increased and the benefits are improved. By using the microbial preparation, the harm caused by disease prevention and treatment by using antibiotics in a traditional calf feeding method is avoided and the microbial preparation is green and harmless.

Description

A kind of preparation method of calf microorganism formulation
Technical field
The present invention relates to technical field of microbial fermentation, and in particular to a kind of preparation method of calf microorganism formulation.
Background technology
Calf diarrhea is had loose bowels also known as calf, can be occurred throughout the year, is a kind of gastrointestinal disease that calf often sends out.Calf is normal After birth 2-3d starts morbidity, development to calf, grows, survives etc. and have a significant impact.When jumpbogroup is raised, calf diarrhea Incidence often reaches 90%-100%, and the death rate reaches as high as more than 50%, and the development to ox aquaculture causes very big threat.Mesh Front conventional treatment method is antibiotic to be added in feed as additive to carry out feeding, although can be reduced to a certain extent The fatal rate of calf diarrhea, but be possible to remain in ox body using antibiotic, and then the qualities such as beef, milk are affected, And Long-Time Service antibiotic can cause pathogen to develop immunity to drugs to work the mischief with ox body.
Microbial technique is widely used in livestock birds health cultivation field, using Tiny ecosystem engineering philosophy, filters out suitable Different livestock and poultry need beneficial microbe strain, through techniques such as compoundings, make probiotics, in adding drinking-water or feed, can To increase raising poultry nutritive, efficiency of feed utilization is improved, improve the speed of growth；Fowl poultry immune ability and disease resistance are remarkably reinforced, are improved Survival rate；Improve the quality of meat, improve product quality.
The microorganism formulation for using at present is mainly used in improving, balancing the gut flora function of calf, exempts from calf is improved Epidemic disease ability aspect is not enough, and calf diarrhea mostly occurs in juvenile stage, and now the physique of ox is more weak, merely by microorganism formulation Regulation be difficult to allow it to recover.
The content of the invention
To solve above-mentioned technical problem, the invention provides a kind of preparation method of calf microorganism formulation.
The present invention is achieved by the following technical programs：
A kind of calf microorganism formulation, the microorganism formulation is made up of following raw material：Complex micro organism fungicide 5-15, Chinese medicine liquid 3-6, wheat bran 12-20, bean powder 8-10, vitamin E 2-5, vitamin C 1-3.
The complex micro organism fungicide is bacillus cereus, yeast, bacillus subtilis, lichens gemma bar by weight ratio Bacterium constitutes.
The yeast is Se-enriched yeast.
Selenium and vitamin C, vitamin E all can be used as antioxidants, and the two complements each other, and can prevent what is caused because of oxidation Aging, the hardening of tissue, the speed that its change can be slowed down at least, and it also has the ability of activated immune system, with reference to Feeding can improve the resistance of calf, promote it to grow.
A kind of preparation method of calf microorganism formulation, the method is：
Bacillus cereus, yeast, bacillus subtilis, the concentration of bacillus licheniformis are 1-3%, cultivate respectively 15-20h, then must be combined bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis mixed culture 8-15h micro- Bacteria agent, adds the Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract and continues to cultivate 10-20h, adds and wheat bran, bean powder, vitamin C, vitamin E are mixed, and extruder grain is obtained.
The Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract are the Radix Astragali, Radix Codonopsis, wilsonii crushing and water-adding ultrasonic wave extraction 15- Then 40min, centrifuging and taking supernatant is further concentrated to the 0.25-0.5 of supernatant volume.
The Radix Astragali, Radix Codonopsis, wilsonii have preferably effect to enhance immunity, carry out after crushing ultrasonic wave extraction, centrifugation, Concentration can obtain higher concentration, be used as needed.
10-30min extruder grains again are dried after the mixing at 30-45 DEG C.
The daily consumption of the microorganism formulation for calf daily ration 0.3-3%.
The microorganism formulation is in calf<Daily consumption is the 0.3-1.2% of calf daily ration during 20 age in days.
The microorganism formulation daily consumption in calf >=20 age in days is the 1.2-3% of calf daily ration.
The consumption of its microorganism formulation is considered according to the age in days of calf, more meets its growth characteristics, because calf is big Little to decide its immunocompetence, the power of stomach ability, such feeding can not only meet its growth demand, also avoid wasting.
The beneficial effects of the present invention is：The present invention ties Chinese medicine extract, vitamin together with complex micro organism fungicide Close, the illnesss such as the function of intestinal canal of calf, alleviating diarrhoea can not only be improved, moreover it is possible to promote calf to grow, and Chinese medicine liquid, vitamin C, vitamin E can improve the resistance of ox, reduce morbidity risk rate, and the yeast for using carries out long-term feeding for Se-enriched yeast can be with As making Niu Zuowei selenium-rich product increase surcharge, increase the benefit.
Specific embodiment
Technical scheme is further described with reference to embodiments, but claimed scope is not limited to In described.
Embodiment one
A kind of calf microorganism formulation, the microorganism formulation is made up of following raw material：Complex micro organism fungicide 5th, Chinese medicine liquid 3, wheat bran 12, bean powder 8, vitamin E 2, vitamin C 1.
Complex micro organism fungicide is bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis group by weight ratio Into.
Yeast is Se-enriched yeast.
A kind of preparation method of calf microorganism formulation, the method is：
Bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis cultivate respectively 15h, then by waxy gemma Bacillus, yeast, bacillus subtilis, bacillus licheniformis mixed culture 8-h obtain complex micro organism fungicide, add the Radix Astragali, party Ginseng, acanthopanax senticosus extract continue to cultivate 10h, add and are mixed with wheat bran, bean powder, vitamin C, vitamin E, extruder grain system .
The Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract are the Radix Astragali, Radix Codonopsis, wilsonii crushing and water-adding ultrasonic wave extraction 15min, are centrifuged Supernatant is taken, the 0.25 of supernatant volume is then further concentrated to.
10min extruder grains again are dried after mixing at 30 DEG C.
Microorganism formulation is in calf<Daily consumption is the 0.3% of calf daily ration during 20 age in days.
Microorganism formulation daily consumption in calf >=20 age in days is the 1.2% of calf daily ration.
Embodiment two
A kind of calf microorganism formulation, the microorganism formulation is made up of following raw material：Complex micro organism fungicide 7th, Chinese medicine liquid 4, wheat bran 14, bean powder 9, vitamin E 3, vitamin C 2.
Complex micro organism fungicide is bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis group by weight ratio Into.
Yeast is Se-enriched yeast.
A kind of preparation method of calf microorganism formulation, the method is：
Bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis cultivate respectively 16h, then by waxy gemma Bacillus, yeast, bacillus subtilis, bacillus licheniformis mixed culture 10h obtain complex micro organism fungicide, add the Radix Astragali, party Ginseng, acanthopanax senticosus extract continue to cultivate 12h, add and are mixed with wheat bran, bean powder, vitamin C, vitamin E, extruder grain system .
The Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract are the Radix Astragali, Radix Codonopsis, wilsonii crushing and water-adding ultrasonic wave extraction 20min, are centrifuged Supernatant is taken, the 0.3 of supernatant volume is then further concentrated to.
15min extruder grains again are dried after mixing at 35 DEG C.
Microorganism formulation is in calf<Daily consumption is the 0.5% of calf daily ration during 20 age in days.
Microorganism formulation daily consumption in calf >=20 age in days is the 1.5% of calf daily ration.
Embodiment three
A kind of calf microorganism formulation, the microorganism formulation is made up of following raw material：Complex micro organism fungicide 9th, Chinese medicine liquid 5, wheat bran 18, bean powder 10, vitamin E 4, vitamin C 3.
Complex micro organism fungicide is bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis group by weight ratio Into.
Yeast is Se-enriched yeast.
A kind of preparation method of calf microorganism formulation, the method is：
Bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis cultivate respectively 18h, then by waxy gemma Bacillus, yeast, bacillus subtilis, bacillus licheniformis mixed culture 12h obtain complex micro organism fungicide, add the Radix Astragali, party Ginseng, acanthopanax senticosus extract continue to cultivate 15h, add and are mixed with wheat bran, bean powder, vitamin C, vitamin E, extruder grain system .
The Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract are the Radix Astragali, Radix Codonopsis, wilsonii crushing and water-adding ultrasonic wave extraction 30min, are centrifuged Supernatant is taken, the 0.35 of supernatant volume is then further concentrated to.
24min extruder grains again are dried after mixing at 40 DEG C.
Microorganism formulation is in calf<Daily consumption is the 0.8% of calf daily ration during 20 age in days.
Microorganism formulation daily consumption in calf >=20 age in days is the 2.1% of calf daily ration.
Example IV
A kind of calf microorganism formulation, the microorganism formulation is made up of following raw material：Complex micro organism fungicide 12nd, Chinese medicine liquid 5, wheat bran 18, bean powder 10, vitamin E 5, vitamin C 3.
Complex micro organism fungicide is bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis group by weight ratio Into.
Yeast is Se-enriched yeast.
A kind of preparation method of calf microorganism formulation, the method is：
Bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis cultivate respectively 20h, then by waxy gemma Bacillus, yeast, bacillus subtilis, bacillus licheniformis mixed culture 15h obtain complex micro organism fungicide, add the Radix Astragali, party Ginseng, acanthopanax senticosus extract continue to cultivate 17h, add and are mixed with wheat bran, bean powder, vitamin C, vitamin E, extruder grain system .
The Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract are the Radix Astragali, Radix Codonopsis, wilsonii crushing and water-adding ultrasonic wave extraction 35min, are centrifuged Supernatant is taken, the 0.4 of supernatant volume is then further concentrated to.
30min extruder grains again are dried after mixing at 45 DEG C.
Microorganism formulation is in calf<Daily consumption is the 1.0% of calf daily ration during 20 age in days.
Microorganism formulation daily consumption in calf >=20 age in days is the 2.7% of calf daily ration.
Embodiment five
A kind of calf microorganism formulation, the microorganism formulation is made up of following raw material：Complex micro organism fungicide 15th, Chinese medicine liquid 6, wheat bran 20, bean powder 10, vitamin E 5, vitamin C 3.
Complex micro organism fungicide is bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis group by weight ratio Into.
Yeast is Se-enriched yeast.
A kind of preparation method of calf microorganism formulation, the method is：
Bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis cultivate respectively 20h, then by waxy gemma Bacillus, yeast, bacillus subtilis, bacillus licheniformis mixed culture 15h obtain complex micro organism fungicide, add the Radix Astragali, party Ginseng, acanthopanax senticosus extract continue to cultivate 20h, add and are mixed with wheat bran, bean powder, vitamin C, vitamin E, extruder grain system .
The Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract are the Radix Astragali, Radix Codonopsis, wilsonii crushing and water-adding ultrasonic wave extraction 40min, are centrifuged Supernatant is taken, the 0.5 of supernatant volume is then further concentrated to.
30min extruder grains again are dried after mixing at 45 DEG C.
Microorganism formulation is in calf<Daily consumption is the 1.2% of calf daily ration during 20 age in days.
Microorganism formulation daily consumption in calf >=20 age in days is the 3% of calf daily ration.
Experimental example one
In same cattle farm, by 36 close calves of the bodily form two groups are divided to, 9 per group, one group uses conventional husbandry methods, i.e., Using antibiotic feeding, another group of method using embodiment one carries out feeding.Raise one month, the growing state of ox is carried out Evaluate.
Two methods of table raise the contrast of calf growing state
As can be known from the above table, the treatment that calf rearing not only contributes to suffer from diarrhoea is carried out using the present invention, and long-term feeding is also The resistance of ox can be strengthened, disease is reduced, the quality of ox is improved.

Claims (9)

1. a kind of calf microorganism formulation, it is characterised in that：The microorganism formulation is made up of following raw material：It is compound micro- Bacteria agent 5-15, Chinese medicine liquid 3-6, wheat bran 12-20, bean powder 8-10, vitamin E 2-5, vitamin C 1-3.
2. a kind of calf microorganism formulation according to claim 1, it is characterised in that：The complex micro organism fungicide by Weight ratio is bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis composition.
3. a kind of calf microorganism formulation according to claim 1, it is characterised in that：The yeast is Se-enriched yeast.
4. a kind of preparation method of calf microorganism formulation, it is characterised in that：The method is：
Bacillus cereus, yeast, bacillus subtilis, bacillus licheniformis cultivate respectively 15-20h, then by waxy gemma Bacillus, yeast, bacillus subtilis, bacillus licheniformis mixed culture 8-15h obtain complex micro organism fungicide, add the Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract continue to cultivate 10-20h, add and are mixed with wheat bran, bean powder, vitamin C, vitamin E, and extruding is made Grain is obtained.
5. the preparation method of a kind of calf microorganism formulation according to claim 4, it is characterised in that：The Radix Astragali, Radix Codonopsis, acanthopanax senticosus extract be the Radix Astragali, Radix Codonopsis, wilsonii crushing and water-adding ultrasonic wave extraction 15-40min, centrifuging and taking supernatant, so The 0.25-0.5 of supernatant volume is further concentrated to afterwards.
6. the preparation method of a kind of calf microorganism formulation according to claim 4, it is characterised in that：After the mixing 10-30min extruder grains again are dried at 30-45 DEG C.
7. the preparation method of a kind of calf microorganism formulation according to claim 4, it is characterised in that：The microorganism The daily consumption of preparation is the 0.3-3% of calf daily ration.
8. the preparation method of a kind of calf microorganism formulation according to claim 7, it is characterised in that：The microorganism Preparation is in calf<Daily consumption is the 0.3-1.2% of calf daily ration during 20 age in days.
9. the preparation method of a kind of calf microorganism formulation according to claim 7, it is characterised in that：The microorganism Preparation daily consumption in calf >=20 age in days is the 1.2-3% of calf daily ration.
CN201611024174.7A 2016-11-16 2016-11-16 Preparation method of microbial preparation for calves Pending CN106615728A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107058396A (en) * 2017-05-31 2017-08-18 南宁学院 A kind of preparation method of ox microorganism formulation
CN107125455A (en) * 2017-05-22 2017-09-05 南宁学院 A kind of 6 monthly age calf composite probiotics preparations and preparation method thereof
CN107683961A (en) * 2017-08-18 2018-02-13 吉林农业大学 One kind utilizes yeast waste liquor production feedstuff mildew water-preserving promoting agent

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102232998A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof
CN103947904A (en) * 2014-05-15 2014-07-30 邵素英 Preparation method of glucose precursor for improving dairy cow rumen flora

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102232998A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof
CN103947904A (en) * 2014-05-15 2014-07-30 邵素英 Preparation method of glucose precursor for improving dairy cow rumen flora

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107125455A (en) * 2017-05-22 2017-09-05 南宁学院 A kind of 6 monthly age calf composite probiotics preparations and preparation method thereof
CN107058396A (en) * 2017-05-31 2017-08-18 南宁学院 A kind of preparation method of ox microorganism formulation
CN107683961A (en) * 2017-08-18 2018-02-13 吉林农业大学 One kind utilizes yeast waste liquor production feedstuff mildew water-preserving promoting agent

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Effective date of registration: 20171220

Address after: Bai Wei Zhen Nanning Shanglin Zhuang village 530000 the Guangxi Zhuang Autonomous Region towards the Korean Wei No. 25 (the operation of Bai Wei Zhen Chao Zhuang Cun Wei Chao)

Applicant after: Guangxi Shanshui cattle husbandry Co., Ltd.

Address before: Dong Ge road Nanning 530022 the Guangxi Zhuang Autonomous Region No. 118 green Wanda Plaza West 1 Building No. 3501

Applicant before: Guangxi Shanshui Agriculture Co., Ltd.

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Application publication date: 20170510