CN111011602A - Functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health and preparation method thereof - Google Patents
Functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health and preparation method thereof Download PDFInfo
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Abstract
The invention provides a functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health and a preparation method thereof, belonging to the field of feed additives. The functional feed additive for relieving the oxidative stress of the livestock and poultry and improving the intestinal health comprises the following raw materials: 50-60 parts of compound fermentation traditional Chinese medicine, 15-20 parts of sodium bicarbonate, 5-10 parts of soybean isoflavone, 3 parts of yeast chromium and 1-2 parts of gamma-aminobutyric acid. The preparation method comprises the following steps: fermenting different Chinese medicinal materials according to recommended formula under combined action of composite bacteria and composite enzyme for 48-72 hr, drying at low temperature, pulverizing, and mixing with other raw materials. The invention adopts a precise compound nutrition technology, comprehensively relieves the oxidative stress of livestock and poultry, improves the intestinal health problem, comprehensively improves the anti-stress capability of animals, increases the production performance of the animals and reduces the breeding loss.
Description
Technical Field
The invention relates to the field of feed additives, in particular to a functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health and a preparation method thereof.
Background
Stress refers to the sum of nonspecific physiological responses of the body due to external environmental stimuli. Oxidative stress is the clearance rate of free radicals in the body exceeds the production rate, the oxidative activity exceeds the oxidation repair capacity of the antioxidant, and the oxidative system and the antioxidant system are unbalanced, so that tissues and cells of the animal body are damaged. At present, the research reports that oxidative stress can induce various diseases such as cardiovascular diseases and the like, and the oxidative stress poses serious threats to the health of animals and human beings. Factors contributing to oxidative stress in animals include: high temperature, high density feeding, group transfer, long-distance transportation, feed mildew, oxidative deterioration, heavy metal exceeding standard, frequent use of antibiotics and other medicines, diseases, immunization, noise and the like.
The capacity of eliminating active oxygen free radicals in vivo can be increased or the generation speed of the active oxygen free radicals can be reduced by adding the antioxidant, the action of a single antioxidant is not ideal in actual production, and the invention mainly realizes the aims of animal health and high-efficiency production by compounding various additives.
Chinese patent publication CN109362967A discloses a plant extract feed additive for relieving pig oxidative stress and improving meat quality and application thereof, wherein the plant extract comprises mangosteen bark extract, grape seed extract, rhodiola rosea extract and schisandra chinensis extract, and is used for improving the productivity of pigs and improving the immune effect. Because the plant extract is extracted by boiling, alcohol dissolving and other processes, compared with the original traditional Chinese medicine, the content of the active ingredients is limited, and the application effect is limited compared with the fermented traditional Chinese medicine.
Chinese patent publication CN105211573A discloses a feed additive premix for alleviating oxidative stress of broiler intestinal tracts and application thereof, which comprises β -glucosidase 20-50g/kg, glucose oxidase 40-80g/kg, vitamin B25-10g/kg, active yeast selenium 15-30mg/kg, chitosan 30-60g/kg and sticktight extract 50-80g/kg, and has the effects of promoting growth of broiler chickens, improving feed utilization rate and improving organism oxidation resistance, and the effect of improving the meat quality of broiler chickens needs to be further verified.
Disclosure of Invention
The invention overcomes the defects in the prior art and provides the functional feed additive for relieving the oxidative stress of livestock and poultry and improving the intestinal health and the preparation method thereof.
The purpose of the invention is realized by the following technical scheme.
A preparation method of a functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health comprises the following steps: the method comprises the following steps:
s1: preparing a composite traditional Chinese medicine: weighing fructus Schisandrae chinensis, Eucommiae cortex, folium Pini, herba Houttuyniae and flos Lonicerae at a certain proportion, pulverizing, and sieving with 30 mesh sieve to obtain composite Chinese medicine;
s2: preparing a solid fermented material: weighing corn flour, bean pulp and the compound traditional Chinese medicine in the S1 in proportion, and sterilizing at high temperature;
s3: activation and inoculation of zymophyte: the bacterial powder is prepared according to the following steps of bacillus subtilis: and (3) saccharomyces cerevisiae: enterococcus faecalis; mixing lactobacillus acidophilus in a ratio of 3:3:1:1, activating, performing step-by-step propagation, and inoculating the lactobacillus acidophilus into the mixed material obtained in the step S2;
s4: solid state fermentation: adding cellulase, xylanase, pectinase and composite amylase into the mixed material obtained in the S3, wherein the proportion of the added water is 40-50% of the total material weight dry weight, performing solid state fermentation for 48-72h, and after the fermentation is finished, the fermented material has fermented fragrance, the water content reaches 39%, the pH is less than or equal to 4.0, and the total viable count is more than or equal to 2 x 108CFU/g；
S5: preparing a compound fermentation traditional Chinese medicine: drying the fermented material in the S4 at low temperature, crushing and sieving with a 40-mesh sieve to obtain the compound fermented traditional Chinese medicine;
s6: preparing a feed additive: weighing the composite fermented traditional Chinese medicine, sodium bicarbonate, soybean isoflavone, yeast chromium and gamma-aminobutyric acid according to the formula proportion, and uniformly mixing.
Further, the weight parts of the materials weighed in the step S1 are as follows: 20-30 parts of schisandra chinensis, 10-20 parts of eucommia ulmoides, 10-15 parts of pine needles, 10-15 parts of houttuynia cordata and 10-15 parts of honeysuckle.
Further, the weight parts of the materials weighed in the step S2 are as follows: 80-85 parts of compound traditional Chinese medicine, 5-10 parts of corn flour and 5-10 parts of bean pulp.
Further, the sterilization temperature in the S2 is 110-120 ℃, and the sterilization time is 30 min.
Further, inoculating bacteria powder in the S3 according to 10-15% of the weight of the S2 mixed material.
The feed additive obtained by the preparation method of the functional feed additive for relieving the oxidative stress of the livestock and improving the intestinal health is characterized in that: the feed additive is prepared by mixing the following components in parts by weight: 50-60 parts of S5 product composite fermentation traditional Chinese medicine, 15-20 parts of sodium bicarbonate, 5-10 parts of soybean isoflavone, 3 parts of yeast chromium and 1-2 parts of gamma-aminobutyric acid.
Further, the feed additive is prepared by mixing the following components in parts by weight: 75 parts of compound fermentation traditional Chinese medicine, 15 parts of sodium bicarbonate, 5 parts of soybean isoflavone, 3 parts of yeast chromium and 2 parts of gamma-aminobutyric acid.
Further, the feed additive is prepared by mixing the following components in parts by weight: 75 parts of compound fermentation traditional Chinese medicine, 13 parts of sodium bicarbonate, 8 parts of soybean isoflavone, 3 parts of yeast chromium and 1 part of gamma-aminobutyric acid.
The invention has the beneficial effects that:
the composite fermented traditional Chinese medicine is prepared by completely releasing effective components in a traditional Chinese medicine formula mainly under the action of microorganisms, adding new active substances and enhancing the treatment, prevention and health care effects of the traditional Chinese medicine. The traditional Chinese medicine fermented by the compound traditional Chinese medicine of the invention contains the following anti-stress components: the contents of flavone, polysaccharide and organic acid are obviously increased. Schisandra chinensis: astringe, benefit qi, promote the production of body fluid, tonify kidney, and calm heart. Has effects in stimulating appetite, invigorating spleen, relieving cough, eliminating phlegm, and reducing stress. Eucommia ulmoides: has effects of nourishing liver and kidney, strengthening tendons and bones, enhancing immunity, and improving immunity and anti-stress ability. Pine needle: is rich in flavonoids, polysaccharides, vitamins and carotene, and has effects of stimulating appetite, invigorating spleen, tranquilizing mind and centering. Houttuynia cordata: contains flavone and houttuynin, and has effects of clearing away heat and toxic materials, promoting urination, treating stranguria, and reducing stress. Honeysuckle flower: clearing away heat and toxic material, has chlorogenic acid as main component, diminishing inflammation, resisting bacteria and strengthening body's immunity.
The sodium bicarbonate is used for resisting heat stress, relieving livestock and poultry acidosis and increasing the alkali storage content of body fluid on one hand; on the other hand, bicarbonate radical (HCO3- -) is a buffer component, and has important significance for maintaining normal acid-base balance and osmotic pressure of the organism.
Soybean isoflavone: is a general term for polyphenol structure mixture separated and extracted from soybean, and the active ingredients mainly comprise daidzein, fuel lignin and glycitein, which can enhance the capability of resisting oxidative stress of animals and improve the immune function and production performance of the animals.
Yeast chromium: chromium is used as an important active ingredient of Glucose Tolerance Factor (GTF), and the utilization rate of energy in glucose and feed is improved by promoting the sensitivity of insulin in combination with tissue cell receptors, so that the synthesis of protein in muscle tissue is increased, the synthesis of fat tissue is reduced, and the anti-stress and immune functions of animals are adjusted.
Gamma-aminobutyric acid: the hypothalamus-pituitary-adrenal axis is the major neuro-endocrine pathway for animals to regulate stress, and gamma-aminobutyric acid can inhibit the secretion of axial hormones under stress conditions.
Compared with the prior art, the feed additive has the advantages that the ① composite feed additive has a more comprehensive anti-stress effect, the stress problem can be solved in multiple ways in animals, ② composite fermentation traditional Chinese medicines meet the requirements of green additives, the feed additive is safe, efficient and free of residues for animals, the drug effect is completely retained by adopting a biological fermentation technology, ③ comprehensively improves the immune function of animals, has a traditional Chinese medicine improving effect on intestinal health, ④ has an adjusting effect on different stress states, ⑤ improves the regulation of animal organisms, obviously improves the meat quality of animal products, ⑥ improves the animal welfare and reduces the breeding loss.
Detailed Description
The technical solution of the present invention is further illustrated by the following specific examples.
A preparation method of a functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health comprises the following steps: the method comprises the following steps:
s1: preparing a composite traditional Chinese medicine: weighing fructus Schisandrae chinensis, Eucommiae cortex, folium Pini, herba Houttuyniae and flos Lonicerae at a certain proportion, pulverizing, and sieving with 30 mesh sieve to obtain composite Chinese medicine;
s2: preparing a solid fermented material: weighing corn flour, bean pulp and the compound traditional Chinese medicine in the S1 in proportion, and sterilizing at high temperature;
s3: activation and inoculation of zymophyte: the bacterial powder is prepared according to the following steps of bacillus subtilis: and (3) saccharomyces cerevisiae: enterococcus faecalis; mixing lactobacillus acidophilus in a ratio of 3:3:1:1, activating, performing step-by-step propagation, and inoculating the lactobacillus acidophilus into the mixed material obtained in the step S2;
s4: solid state fermentation: adding cellulase, xylanase, pectinase and composite amylase into the mixed material obtained in the S3, wherein the proportion of the added water is 40-50% of the total weight and dry weight of the material, and performing solid state fermentation for 48-72 h;
s5: preparing a compound fermentation traditional Chinese medicine: drying the fermented material in the S4 at low temperature, crushing and sieving with a 40-mesh sieve to obtain the compound fermented traditional Chinese medicine;
s6: preparing a feed additive: weighing the composite fermented traditional Chinese medicine, sodium bicarbonate, soybean isoflavone, yeast chromium and gamma-aminobutyric acid according to the formula proportion, and uniformly mixing.
Further, the weight parts of the materials weighed in the step S1 are as follows: 20-30 parts of schisandra chinensis, 10-20 parts of eucommia ulmoides, 10-15 parts of pine needles, 10-15 parts of houttuynia cordata and 10-15 parts of honeysuckle.
Further, the weight parts of the materials weighed in the step S2 are as follows: 80-85 parts of compound traditional Chinese medicine, 5-10 parts of corn flour and 5-10 parts of bean pulp.
Further, the sterilization temperature in the S2 is 110-120 ℃, and the sterilization time is 30 min.
Further, inoculating bacteria powder in the S3 according to 10-15% of the weight of the S2 mixed material.
Example 1
A functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health comprises the following raw materials in parts by weight: 75 parts of compound fermentation traditional Chinese medicine prepared by the method, 15 parts of sodium bicarbonate, 5 parts of soybean isoflavone, 3 parts of yeast chromium and 2 parts of gamma-aminobutyric acid, wherein the compound fermentation traditional Chinese medicine comprises the following components: 25 parts of schisandra chinensis, 15 parts of eucommia ulmoides, 15 parts of pine needles, 10 parts of houttuynia cordata and 10 parts of honeysuckle.
The application effect of the functional feed additive for relieving the oxidative stress of livestock and poultry and improving the intestinal health on the Roman powder laying hens is verified. The applicant performs tests in a certain hen farm of the three gorges in the south of the Henan, selects 300-day-old Roman powder hens in a 3-span hen house to perform the tests, and the test period is 8 weeks, wherein the test period comprises a normal-temperature control group (the temperature is 23-25 ℃ and the humidity is about 65%), a high-temperature control group (the temperature is 32 ℃ and the humidity is 70-75%) and a test group (the temperature is 32 ℃ and the humidity is 70-75%), the normal-temperature control group and the high-temperature control group are used for feeding basic egg laying materials, and the test group feed is a basic feed plus 0.5% of additives. The test time is 5 and 25 days in 2019 and 7 and 20 days in 2019.
The feeding management is operated according to the normal standard of the farm. And recording the egg laying number, the total egg laying weight, the broken egg number and the dead egg number of the laying hens every day.
Laying rate (%) - (egg production quantity/number of eggs laid in a house/number of feathers of a laying hen
Egg weight (g/egg) is the total weight of eggs/total number of eggs
The ratio of feed to egg is total feeding amount/total egg weight
The test data were statistically analyzed using SPSS12.0 statistical software, and the results were expressed as "mean. + -. standard deviation" by single factor variance comparison.
The test results are shown in the following tables 1, 2 and 3:
table 1: influence of additive on production performance of Roman powder laying hens in different treatment groups
| Group of | Laying rate (%) | Average egg weight (g) | Percentage of broken eggs (%) | Material to egg ratio | Death rate (%) |
| Control group at normal temperature | 89.5±3.02a | 57.18±0.48 | 1.31±0.05b | 2.39±0.15b | 1.01±0.08b |
| High temperature control group | 81.3±2.18b | 55.03±0.54 | 3.57±0.03a | 2.56±0.11a | 2.85±0.05a |
| Test group | 89.9±1.99a | 58.01±0.56 | 0.96±0.03c | 2.31±0.11b | 0.99±0.07c |
From the above table, the productivity of the high temperature control group is significantly reduced compared with the normal temperature control group, and the productivity of the test group is improved to a greater extent, which is better than the normal temperature control group. The laying rate, the egg breaking rate, the feed-egg ratio and the death and culling rate of the normal-temperature control group and the test group are all obviously superior to those of the high-temperature control group.
Table 2: influence of additive on egg quality of Roman powder laying hens in different treatment groups
The above table shows that the additive disclosed by the invention can relieve the quality reduction of eggs of laying hens under a high-temperature high-humidity stress state, and obviously improves the transportation and long-distance storage of the eggs.
Table 3: influence of additive on intestinal villus height of Roman powder laying hens in different treatment groups
| Group of | Height of duodenum villi (mum) | Jejunum villus height (mum) | Ileum villus height (mum) |
| Control group at normal temperature | 940.57±13.57a | 609.18±19.54a | 590.32±18.96a |
| High temperature control group | 822.15±21.86b | 478.66±18.65b | 470.54±19.55b |
| Test group | 944.63±20.65a | 609.15±15.30a | 588.6±10.81a |
As can be seen from the above table, the villi height of duodenum, jejunum and ileum of the test group and the normal temperature control group is obviously higher than that of the high temperature control group, which shows that the feed additive of the invention can protect the intestinal tract from being damaged under the stress state, increase the absorption area of the intestinal tract and improve the immune function and the production performance of animals.
Example 2
A functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health comprises the following raw materials in parts by weight: 75 parts of compound fermentation traditional Chinese medicine prepared by the method, 13 parts of sodium bicarbonate, 8 parts of soybean isoflavone, 3 parts of yeast chromium and 1 part of gamma-aminobutyric acid, wherein the compound fermentation traditional Chinese medicine comprises the following components in parts by weight: 30 parts of schisandra chinensis, 15 parts of eucommia ulmoides, 10 parts of pine needles, 10 parts of houttuynia cordata and 10 parts of honeysuckle.
The functional feed additive for relieving the oxidative stress of the livestock and poultry and improving the intestinal health has the effect of improving the production performance and the intestinal health of the fattening pigs under the stress condition, and an applicant performs a test in a certain farm in a chatting city of Shandong province. The test date is 2019, 5-7 months, and the test formal period is 56 days. The additive is used in an amount of 0.1%, and the basic feed is fattening pig feed. 150 fattening pigs are selected for test, and divided into a normal temperature control group (the temperature is 23-25 ℃ and the humidity is about 65%), a high temperature control group (the temperature is 32 ℃ and the humidity is 70-75%) and a test group (the temperature is 32 ℃ and the humidity is 70-75%). The normal temperature control group and the high temperature control group are fed with basic feed, and the test group is added with 0.1% of additive on the basic feed.
And (4) performing operation on the experimental feeding management according to normal regulations of a farm. The production performance is as follows: measuring average initial weight, final weight, daily feeding amount, residual amount and feed intake, and calculating material-weight ratio.
After the test is finished, fattening pigs with two weights equal to the average weight are selected for each group, ear veins are collected in a heparin anticoagulation tube, blood plasma is separated by centrifugation at 3000r/min for 10min, and the mixture is stored in a refrigerator at minus 20 ℃. The kit is used to determine the malondialdehyde content, total antioxidant capacity and total superoxide dismutase and catalase activity in plasma and muscle.
The test data were statistically analyzed using SPSS12.0 statistical software, and the results were expressed as "mean. + -. standard deviation" by single factor variance comparison.
The test results are shown in tables 4, 5 and 6 below:
table 4: influence of additive on the production performance of fattening pigs of different treatment groups
The above table shows that the additive of the invention added with 0.1 percent can relieve the stress of high temperature to fattening pigs, maintain and improve the production performance of the fattening pigs, the daily gain and the feed intake are obviously higher than those of a high-temperature control group, and the feed-weight ratio is obviously lower than that of the high-temperature control group.
Table 5: influence of additive on oxidative stress indexes of serum of fattening pigs in different treatment groups
As can be seen from the table above, the addition of 0.1% of additive can significantly improve the antioxidant capacity of the fattening pig serum, significantly reduce the content of malondialdehyde in the serum, and significantly increase the content of total superoxide dismutase, total antioxidant capacity and catalase.
Table 6: influence of additive on oxidative stress indexes of longissimus dorsi of fattening pigs in different treatment groups
From the above table, it can be seen that the addition of 0.1% of additive can significantly improve the antioxidant capacity of the muscle of the fattening pig, significantly reduce the content of malondialdehyde in serum, and significantly increase the content of total superoxide dismutase, total antioxidant capacity and catalase.
It will be understood by those skilled in the art that the functional feed additive for alleviating oxidative stress and improving intestinal health of livestock and poultry and the preparation method thereof according to the present invention comprise any combination of the above-mentioned summary of the invention and the parts shown in the detailed description of the invention, which is limited by space and not described in any of the schemes comprising the combination for the sake of brevity. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A preparation method of a functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health is characterized by comprising the following steps: the method comprises the following steps:
s1: preparing a composite traditional Chinese medicine: weighing fructus Schisandrae chinensis, Eucommiae cortex, folium Pini, herba Houttuyniae and flos Lonicerae at a certain proportion, pulverizing, and sieving with 30 mesh sieve to obtain composite Chinese medicine;
s2: preparing a solid fermented material: weighing corn flour, bean pulp and the compound traditional Chinese medicine in the S1 in proportion, and sterilizing at high temperature;
s3: activation and inoculation of zymophyte: the bacterial powder is prepared according to the following steps of bacillus subtilis: and (3) saccharomyces cerevisiae: enterococcus faecalis; mixing lactobacillus acidophilus in a ratio of 3:3:1:1, activating, performing step-by-step propagation, and inoculating the lactobacillus acidophilus into the mixed material obtained in the step S2;
s4: solid state fermentation: adding cellulase, xylanase, pectinase and composite amylase into the mixed material obtained in the S3, wherein the proportion of the added water is 40-50% of the total material weight dry weight, performing solid state fermentation for 48-72h, and after the fermentation is finished, the fermented material has fermented fragrance, the water content reaches 39%, the pH is less than or equal to 4.0, and the total viable count is more than or equal to 2 x 108CFU/g；
S5: preparing a compound fermentation traditional Chinese medicine: drying the fermented material in the S4 at low temperature, crushing and sieving with a 40-mesh sieve to obtain the compound fermented traditional Chinese medicine;
s6: preparing a feed additive: weighing the composite fermented traditional Chinese medicine, sodium bicarbonate, soybean isoflavone, yeast chromium and gamma-aminobutyric acid according to the formula proportion, and uniformly mixing.
2. The preparation method of the functional feed additive for relieving the oxidative stress and improving the intestinal health of the livestock and the poultry according to claim 1, which is characterized by comprising the following steps: the weight parts of the materials weighed in the S1 are as follows: 20-30 parts of schisandra chinensis, 10-20 parts of eucommia ulmoides, 10-15 parts of pine needles, 10-15 parts of houttuynia cordata and 10-15 parts of honeysuckle.
3. The preparation method of the functional feed additive for relieving the oxidative stress and improving the intestinal health of the livestock and the poultry according to claim 1, which is characterized by comprising the following steps: the weight parts of the materials weighed in the S2 are as follows: 80-85 parts of compound traditional Chinese medicine, 5-10 parts of corn flour and 5-10 parts of bean pulp.
4. The preparation method of the functional feed additive for relieving the oxidative stress and improving the intestinal health of the livestock and the poultry according to claim 2, which is characterized by comprising the following steps: the sterilization temperature in the S2 is 110-120 ℃, and the sterilization time is 30 min.
5. The preparation method of the functional feed additive for relieving the oxidative stress and improving the intestinal health of the livestock and the poultry according to claim 1, which is characterized by comprising the following steps: inoculating bacterial powder which accounts for 10-15% of the weight of the S2 mixed material into the S3.
6. The feed additive obtained by the preparation method of the functional feed additive for relieving the oxidative stress and improving the intestinal health of the livestock and the poultry according to any one of claims 1 to 5 is characterized in that: the feed additive is prepared by mixing the following components in parts by weight: 50-60 parts of S5 product composite fermentation traditional Chinese medicine, 15-20 parts of sodium bicarbonate, 5-10 parts of soybean isoflavone, 3 parts of yeast chromium and 1-2 parts of gamma-aminobutyric acid.
7. The feed additive of claim 6, wherein: the feed additive is prepared by mixing the following components in parts by weight: 75 parts of compound fermentation traditional Chinese medicine, 15 parts of sodium bicarbonate, 5 parts of soybean isoflavone, 3 parts of yeast chromium and 2 parts of gamma-aminobutyric acid.
8. The feed additive of claim 6, wherein: the feed additive is prepared by mixing the following components in parts by weight: 75 parts of compound fermentation traditional Chinese medicine, 13 parts of sodium bicarbonate, 8 parts of soybean isoflavone, 3 parts of yeast chromium and 1 part of gamma-aminobutyric acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911407175.3A CN111011602A (en) | 2019-12-31 | 2019-12-31 | Functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health and preparation method thereof |
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| CN111528351A (en) * | 2020-06-09 | 2020-08-14 | 吴建东 | Traditional Chinese medicine probiotic feed additive and preparation method and use method thereof |
| CN111567682A (en) * | 2020-05-22 | 2020-08-25 | 开封嘉骏生物科技有限公司 | Natural feed and enzyme preparation compound type functional additive and preparation method thereof |
| CN112868930A (en) * | 2021-03-01 | 2021-06-01 | 广东大广生物科技有限公司 | Heat stress improvement and growth strengthening fattening pig fermentation material and fermentation device thereof |
| CN116849309A (en) * | 2023-09-05 | 2023-10-10 | 成都铁骑力士饲料有限公司 | Feed additive for improving heat stress resistance of laying hens, preparation method and feed |
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