CN101874812B - Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea - Google Patents
Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea Download PDFInfo
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- 241000894006 Bacteria Species 0.000 title claims abstract description 63
- 238000002360 preparation method Methods 0.000 title claims abstract description 55
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- 206010012735 Diarrhoea Diseases 0.000 title abstract description 14
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- 241000194032 Enterococcus faecalis Species 0.000 claims description 5
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Abstract
The invention relates to a composite bacteria preparation and application thereof in the weight increment of immature livestock and the control of diarrhea. By adding the composite bacteria preparation in feeds, the diarrhea disease of the immature livestock can be effectively controlled, the survival ratio and the breeding benefit of the immature livestock can be improved, and the treatment and control costs of the immature livestock diseases can be saved. The composite bacteria preparation can effectively reduce the antibiotic content in the adult marketing livestock and products thereof and is an effective and reliable way for realizing green breeding and producing green animal products.
Description
Technical field
The invention belongs to biological technical field, be specifically related to composite bacteria preparation and the application in weightening finish of poult poultry and control diarrhea thereof.
Background technology
The drug residue that abuse of antibiotics causes, make human edible the food of drug residue is arranged after, allergy, teratogenesis often occur, disable, acute or chronic disease such as carcinogenic, poisoning.The Along with people's growth in the living standard reaches the increase to the green health food demand, and the drug residue problem of using microbiotic to cause more and more comes into one's own.No drug residue, pollution-free and feeding micro-ecological preparation that can increase economic efficiency become the focus of domestic and international research.Feeding micro-ecological preparation is one type and is exclusively used in and is added in the animal-feed; To improve the production performance and the economic benefit of animal products; Simultaneously can replace traditional Antibiotic Additive again; Have one type of preparation of disease-resistant, no drug residue, pollution-free characteristic concurrently, be realize green cultivation with produce the green animal product effectively, reliable approach.
Bacillus cereus (formal name used at school: Bacillus cereus); Claim the Root and stem of Cholla bacillus again; Being bacillus, is a kind of region, the soil life; Gram-positive rod-shaped bacterium, (1) bacillus cereus produces the various active material in process of growth have obvious suppression to appoint to pathogenic bacterium or conditioned pathogen; Simultaneously bacillus cereus can consume the free oxygen in the environment in the digestive tube rapidly, forms the enteron aisle low-oxygen environment, promotes useful anerobes growth, and produces organic acid such as lactic acid, reduces enteron aisle pH value, suppresses the growth of other pathogenic bacterium indirectly; (2) bacillus cereus can stimulate growing of animal immune organ, and activated lymphocyte improves Tegeline and antibody horizontal; Strengthen cellular immunization and humoral immune function; Improve herd immunity. (3) bacillus cereus can secrete various proteolytic enzyme. glycase. lypase. cellulase etc., in digestive tube, play a role jointly with endogenous enzyme, improve feed digestibility; Bacillus cereus can be synthesized multivitamin simultaneously; Improve the activity of animal body internal interference element and scavenger cell. this bacterium can be regulated the microbial population of animal intestinal tract balance, improves the enteric microorganism ecotope, effectively keeps animal body healthy state; Improve digestive utilization ratio and the ability enhance immunity power of animal, anti-stress ability to feed; Purify the livestock growing environment, reduce predisposing factors, effectively reduce discharge of harmful gases, greatly reduce the respiratory tract disease incidence that causes because of obnoxious flavour; It is natural green feed additive product.
Streptococcus faecium (E.faecalis) and streptococcus faecalis (E.feacium) all belong to enterococcus spp (Enterococcus), are people and animal intestinal normal microflora, and China's approval can be used as fodder additives.This two strains bacterium can be used for preventing and treating the cub fledgling poultry because of the diarrhea that alteration of intestinal flora causes, has the effect of regulating the normal intestinal flora imbalance and promoting growth.
Summary of the invention
The technical problem that the present invention will solve is a control cub fowl diarrhea, can accomplish through composite fungus agent is provided, and said composite fungus agent contains bacillus bacterium and enterococcus spp bacterium.
One of the present invention preferred aspect, said bacillus bacterium is selected from the bacterial strain of bacillus cereus kind or the compsn of different strains.
Said bacillus cereus kind can be available from mountain city, Hainan medicine trade Co., Ltd, and Shijiazhuang Noah prestige Bioisystech Co., Ltd also can obtain from Chinese common micro-organisms culture presevation administrative center, and its preserving number is 1.1626,1.126,1.1483,1.984; 1.932,1.895,1.447,1.352,1.348,1.260,1.230; 1.229,1.2238,1.2224,1.2223,1.196,1.195; 1.189,1.173,1.1846,1.1689,1.1688,1.1687 or 1.1686.
Again more preferably, said bacillus bacterium is for being preserved in Chinese common micro-organisms culture presevation administrative center, and preserving number is 1.1626 bacillus cereus bacterial classification.
This preserving number is that 1.1626 bacterial classification is recorded in 1980 112 volumes of european journal of biological chemistry (Eur.J.Biochem.) 273-281 page or leaf.
One of the present invention preferred aspect, said enterococcus spp bacterium is the bacterial strain of enterococcus faecalis kind or the compsn of different strains.
Said enterococcus faecalis can be available from the green world, Hubei bio tech ltd, the sharp next biological Engineering stock Co., Ltd of Shandong Bora, Xinyi, Shandong biotechnology ltd, the little shield bio tech ltd in Guangzhou; Bailinkangyuan Biotechnology Co., Ltd., Beijing also can obtain from Chinese common micro-organisms culture presevation administrative center, and its preserving number is 1.131; 1.130,1.125,1.101; 1.100,1.595,1.2135 or 1.2024.
Again more preferably, said enterococcus spp bacterium is for being preserved in Chinese common micro-organisms culture presevation administrative center, and preserving number is the compsn of 1.100 or 1.125 streptococcus faecium bacterial strain.
Of the present invention one preferred aspect, contain in the said composite bacteria preparation and be preserved in Chinese common micro-organisms culture presevation administrative center, preserving number is 1.1626 bacillus cereus bacterial classification, preserving number is 1.100 or 1.125 streptococcus faecium.
Said streptococcus faecium 1.100 strains can be available from the green world, the Hubei bio tech ltd that is positioned at Hanchuan City economic and technological development zone north bridge sci-tech industrial park.
Said streptococcus faecium 1.125 strains can come biological Engineering stock Co., Ltd available from the Shandong Bora profit that is positioned at Tai, Shandong Province Taishan District,
Said bacillus cereus 1.1626 strains can be available from mountain city, Hainan medicine trade Co., Ltd that is positioned at the Haikou City, Hainan Province.
Alternatively, this composite bacteria preparation also comprises vehicle, and said vehicle can be selected from the group of common stone flour, Paris white, wheat bran, chaff, or its compsn.Said stone flour is preferably Paris white.
The present invention also provides the cultural method of said bacterial strain:
The enterococcus spp bacterium is with the milk-acid bacteria substratum, and the bacillus bacterium is cultivated by usual method with ordinary broth.
Streptococcus faecium bacterium number is 10 in the single bacterium culture of enterococcus spp bacterium that every gram cultivation obtains
10～10
12Cfu, the bacterium number is 3 * 10 in the single bacterium culture of bacillus bacterium
8～1 * 10
11Cfu.Said bacterial strain all adopts the flat band method counting.
The present invention also provides the method for the said composite bacteria preparation of preparation, may further comprise the steps:
1) strain culture and vehicle are processed dry powder, wherein the mass ratio of strain cultures and vehicle is 1～30: 1, is preferably 10: 1.
2) enterococcus spp microbial inoculum dry powder and bacillus microbial inoculum dry powder are processed composite bacteria preparation by the mixed of mass ratio 1: 0.5～2.The preserving number that is preferably the enterococcus spp bacterium again and is streptococcus faecium is that the preserving number of 1.125 bacterial strain and streptococcus faecium is the compsn of 1.100 bacterial strain, and the bacillus bacterium is that preserving number is 1.1626 bacillus cereus.The preserving number of streptococcus faecium is 1.125 bacterial strain dry powder; The preserving number of streptococcus faecium is that 1.100 bacterial strain dry powder and preserving number are that 1.1626 bacillus cereus agent dry powder is processed composite bacteria preparation in the ratio of mass ratio 1: 0.8～1.2: 1.8～2.2, is preferably 1: 1: 2.
The application that the present invention also provides said composite bacteria preparation to be used to resist the relevant germ of poult animal diseases comprises that white dysentery Salmonellas and piglet are yellow, the dysentery characterized by white mucous stool intestinal bacteria, the dysentery characterized by white mucous stool pathogenic colon bacillus.
The application that the present invention also provides said composite bacteria preparation to be used to prevent and treat the poult animal diseases comprises chick dysentery characterized by white mucous stool, yellow, the dysentery characterized by white mucous stool of piglet.
The purposes that the present invention also provides said composite bacteria preparation to be used for animal weightening finish aspect is mainly used in piglet, chick.
When the composite bacteria preparation that the present invention relates to was used to resist the purposes of the relevant germ of poult animal diseases or control poult animal diseases and animal weightening finish aspect, form that can fodder additives was used, and the consumption of fodder additives is 0.5%～1% of a feed weight.
The bacterial strain that the present invention relates to and the composite bacteria preparation of composition thereof have following advantage:
1) through in feed, adding the composite bacteria preparation that the present invention relates to, can prevent and treat poult poultry dysentery down effectively, improve poult poultry surviving rate, practice thrift the sick treatment of poult epidemic disease of domestic animals prevention and control cost, improve culture benefit.
2) in the livestock culture process; The composite bacteria preparation that the application of the invention relates to; Can avoid the use of antibiotics, can effectively reduce the antibiotic content of delivering for sale in adult fowl poultry and the goods thereof, be to realize green cultivation and effective, the reliable approach of producing the green animal product.
3) composite bacteria preparation that the present invention relates to has no side effect, and helps avoid because the potential spinoff risk of using microbiotic to bring.
4) in the livestock culture process, the composite bacteria preparation that the application of the invention relates to can promote the weightening finish of fowl poultry, practices thrift feed Ingredient, improves culture benefit.
5) the present invention can effectively control baby pig diarrhoea, and is more effective and more stable than preparations such as single use milk-acid bacterias.
Embodiment
The concrete embodiment of following reference explains the present invention.It will be appreciated by those skilled in the art that these embodiment only are used to explain the present invention, the scope that it does not limit the present invention in any way.
The preparation of embodiment 1, microbial inoculum
Prepared the composite bacteria preparation that contains streptococcus faecium 1.100 strains and streptococcus faecium 1.125, bacillus cereus 1.1626 strains.
Said streptococcus faecium 1.100 strains can be available from the green world, the Hubei bio tech ltd that is positioned at Hanchuan City economic and technological development zone north bridge sci-tech industrial park.
Said streptococcus faecium 1.125 strains can come biological Engineering stock Co., Ltd available from the Shandong Bora profit that is positioned at Tai, Shandong Province Taishan District,
Said bacillus cereus 1.1626 strains can be available from mountain city, Hainan medicine trade Co., Ltd that is positioned at the Haikou City, Hainan Province.
Its preparation method is:
1, three kinds of strain cultures and Paris white are processed dry powder, wherein the mass ratio of strain cultures and Paris white is 10: 1;
2, with streptococcus faecium 1.100 strain dry powder, streptococcus faecium 1.125 dry powder and bacillus cereus 1.1626 strain dry powder are pressed 1: 1: 2 mixed of mass ratio, process composite bacteria preparation.
Embodiment 2, to the influence of weight gain of piglets
The weightening finish aspect is mended food according to sucking piglets since 20 ages in days, the situation of 45 ages in days ablactation, and we carry out feeding experiment in this period with the feed that contains the composite bacteria preparation that the present invention relates to.
In the present embodiment; Adopted tri-crossbreeding; The painted face in Beijing opera pig; Jiang Qu sea pig and new Huaihe River pig are all purchased in Wan Shan farm, Jurong City, Jiangsu Province, and wherein tri-crossbreeding is to hybridize the second filial generation of being given birth to the Berkshire boar again by the first-filial generation sow that southern Jiangsu painted face in Beijing opera sow and Yorkshire boar bear.
To carried out 6 tests with the pig young baby who raises variety, the result sees table 2.The many weightening finishes 27.87% of test group average specific control group, far above external with the report (on average about 10%) of probiotic bacterium as the fodder additives weight gain of piglets.(seeing table 2)
The cud crude zyme preparation and the sodium acetate that use in the present embodiment: can be available from physiology teaching and research group of Agricultural University Of Nanjing.Aerobic Bacillus cereus microbial inoculum can be available from Sichuan Agricultural University.
Table 1,6 experiment situation
1. bracket inner digital is a piglet nest number, as 52 (5) promptly 52 piglets adhere to 5 nests separately, down with.
2. add the thick enzyme of cud of massfraction 1% and 2% sodium acetate in the feed.
3. add massfraction 1% aerobic Bacillus cereus microbial inoculum in the feed.
Table 2,6 experiment weightening finish situation
Embodiment 3, control baby pig diarrhoea
Aspect the control baby pig diarrhoea, test shows (seeing table 3), uses the present invention can control baby pig diarrhoea, and more effective, more stable than preparations such as single use milk-acid bacterias.
Table 3, composite bacteria preparation control baby pig diarrhoea effect
* the outer number of bracket is baby pig pujos blancos, and bracket inner digital is a yellow scour of piglet.
Embodiment 4, the influence that prevents and treats the chick diarrhea and chick is increased weight
Chick is selected red POLO and star POLO for use, can be available from animal medicine institute of Agricultural University Of Nanjing attached test pasture.Each employed chick quantity of test and kind are as shown in table 4.The employed microbial inoculum of each test is as shown in table 5.Employed composite bacteria preparation is a prepared composite bacteria preparation among the embodiment 1.
1, with the composite bacteria preparation 1-30 age in days chick (seeing table 6) of feeding, improve weightening finish 15.66% than control group, be higher than other two kinds of preparations in this test, also be higher than abroad and improve the report (on average about 10%) that chick increases weight with probiotics.
2, improve the surviving rate of chick with composite bacteria preparation, the effect of (seeing table 7) is comparatively obvious in 7 tests, average out to 94.47%, and average 81.88% apparently higher than control group, this maybe be relevant with composite bacteria preparation enhancing body resistibility.
3, test of many times shows (seeing table 7), and composite bacteria preparation is prevented and treated the chick dysentery characterized by white mucous stool has positive effect, as fodder additives may command chick dysentery characterized by white mucous stool, to preventing and treating chick coccidiosis positive effect is also arranged with it.
This shows that through long-term a large amount of experiments and use proof, the present invention has unusual effect to the control of chick dysentery characterized by white mucous stool, piglet Huang, dysentery characterized by white mucous stool etc., and gaining effect is arranged.
Table 4, test chicken grouping situation
Table 5, test microbial inoculum service condition
* refer to that microbial inoculum accounts for quality of the fodder per-cent
Table 6, seven tests of chick weightening finish situation
Table 7, chick livability, diarrhea morbidity statistics
Claims (14)
1. composite bacteria preparation; Comprise bacillus bacterium and enterococcus spp bacterium; Said bacillus bacterium is the bacterial strain of bacillus cereus kind bacterium or the compsn of different strains; Said bacillus bacterium is Chinese common micro-organisms bacterial classification keeping reason center for the preservation place, the bacillus cereus of preserving number 1.1626; Said enterococcus spp bacterium is the bacterial strain of enterococcus faecalis kind bacterium or the compsn of different strains; Said enterococcus spp bacterium is Chinese common micro-organisms bacterial classification keeping reason center for the preservation place; Preserving number is two kinds of streptococcus faecium of 1.100 and 1.125, and preserving number is that 1.100 and 1.125 two kinds of streptococcus faecium and preserving number are that the mass ratio of 1.1626 bacillus cereus is 1: 1: 2 in the said composite bacteria preparation.
2. preparation as claimed in claim 1, wherein composite bacteria preparation use bacterium number is every gram 10
10～10
12Single bacterium culture of the enterococcus spp bacterium of cfu and bacterium number are every gram 3 * 10
8～1 * 10
11The bacillus bacterium culture preparation of cfu.
3. preparation as claimed in claim 1 wherein also comprises vehicle.
4. preparation as claimed in claim 3, wherein vehicle can be selected from stone flour, wheat bran, chaff or its compsn.
5. preparation as claimed in claim 4, wherein stone flour is preferably Paris white.
6. the method for preparing the described preparation of claim 3, comprising following steps:
1) strain culture of enterococcus spp bacterium and bacillus bacterium is processed dry powder with vehicle respectively.
2) dry powder blend of strain culture being processed is processed composite bacteria preparation.
7. method as claimed in claim 6, wherein the mass ratio of strain culture and vehicle is 1～30: 1.
8. method as claimed in claim 6, wherein the mass ratio of strain culture and vehicle is 10: 1.
9. method as claimed in claim 6; Wherein the enterococcus spp bacterium is that the preserving number of streptococcus faecium is that the preserving number of 1.125 bacterial strain and streptococcus faecium is the compsn of 1.100 bacterial strain; The bacillus bacterium is that preserving number is 1.1626 bacillus cereus; The preserving number of streptococcus faecium is 1.125 bacterial strain dry powder, and the preserving number of streptococcus faecium is that 1.100 bacterial strain dry powder and preserving number are that 1.1626 bacillus cereus agent dry powder is processed composite bacteria preparation in 1: 1: 2 ratio of mass ratio.
10. the described preparation of claim 1 is used for preparing the application of the medicine of preventing and treating the poult animal diseases, and wherein said poult animal diseases comprises chick dysentery characterized by white mucous stool, yellow scour of piglet, baby pig pujos blancos.
11. the described preparation of claim 1 is used for preparing the application of the medicine of resisting the relevant germ of poult animal diseases, the relevant germ of wherein said poult animal diseases comprises the dysentery characterized by white mucous stool pathogenic colon bacillus.
12. application as claimed in claim 11, wherein said poult poultry can be selected from piglet, chick.
13. the described preparation of claim 1 is used for the purposes of animal weightening finish aspect, wherein said animal can be selected from piglet, chick.
14. like the arbitrary described application of claim 11-13, wherein said preparation uses with the fodder additives form, its consumption is 0.5%～1% of a feed weight.
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| CN102294017B (en) * | 2011-08-01 | 2012-07-11 | 湖南农业大学 | Probiotics oral liquid for animal and preparation method thereof |
| CN102293349A (en) * | 2011-09-09 | 2011-12-28 | 合肥工业大学 | Composite probiotic meat chicken feed additive and application thereof |
| CN105110489A (en) * | 2015-10-10 | 2015-12-02 | 中国水产科学研究院淡水渔业研究中心 | Water-purifying and weed-protecting biological agent for shrimp and crab culture in high-temperature period as well as preparation method and application of biological agent |
| CN107629981B (en) * | 2017-10-13 | 2019-09-27 | 安徽农业大学 | A kind of compound probiotic agent and preparation method thereof improving chick function of intestinal canal |
| CN113368155A (en) * | 2021-06-25 | 2021-09-10 | 山东鲁冠生物科技有限责任公司 | Forsythia suspense probiotic fermentation product and application thereof in preparation of medicines for preventing and treating livestock and poultry viral diseases |
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| CN101263862A (en) * | 2008-05-05 | 2008-09-17 | 大连医立微生态科技开发中心 | Food and rink castoff feed processing method and culture medium and confecting method thereby |
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| CN1333023A (en) * | 2000-07-06 | 2002-01-30 | 吉林省威特集团生化药业有限责任公司 | Bifidoquater live bacterial chip |
| CN2732243Y (en) * | 2004-06-25 | 2005-10-12 | 刘维 | Live Bifidobacterium, Lactobacillus, Enterococcus and Bacillus ceresu composite capsule |
| CN101112397A (en) * | 2007-08-09 | 2008-01-30 | 天津科技大学 | Viable prebiotics bacteria microcapsule preparations for livestock and method for preparing the same |
| CN101263862A (en) * | 2008-05-05 | 2008-09-17 | 大连医立微生态科技开发中心 | Food and rink castoff feed processing method and culture medium and confecting method thereby |
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