CN113368155A - Forsythia suspense probiotic fermentation product and application thereof in preparation of medicines for preventing and treating livestock and poultry viral diseases - Google Patents
Forsythia suspense probiotic fermentation product and application thereof in preparation of medicines for preventing and treating livestock and poultry viral diseases Download PDFInfo
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- CN113368155A CN113368155A CN202110709589.2A CN202110709589A CN113368155A CN 113368155 A CN113368155 A CN 113368155A CN 202110709589 A CN202110709589 A CN 202110709589A CN 113368155 A CN113368155 A CN 113368155A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention relates to fructus forsythiae probiotic fermentation products and application thereof in preparing medicaments for preventing and treating livestock and poultry viral diseases.
Description
Technical Field
The invention belongs to the technical field of biological medicines, and particularly relates to a forsythia suspense probiotic fermentation product and application thereof in preparation of a medicine for preventing and treating livestock and poultry viral diseases.
Background
Forsythia suspensa is a dry fruit of Forsythia subsensis (Latin name) which is a plant of Oleaceae and is mainly produced in Shaanxi, Shanxi, Henan, Shandong and other places in China. Bitter in taste and slightly cold in nature, entering lung, heart and small intestine meridians. Has the effects of clearing away heat and toxic materials, relieving swelling, dispersing pathogenic accumulation, and dispelling pathogenic wind and heat. It can be used for treating carbuncle, cellulitis, scrofula, mammary abscess, erysipelas, wind-heat type common cold, early stage epidemic febrile disease, warm invasion of nutrient, hyperpyrexia, polydipsia, coma, macula, stranguria with astringency, etc. Pharmacological research shows that the forsythia has the activities of resisting oxidation, bacteria, inflammation, virus, tumor, heat and pain, and the like. In modern clinic, it is a common medicine for treating pneumonia, typhoid fever, dysentery, tonsillitis, scrofula, pyocutaneous disease, etc. Fructus forsythiae is rich in lignans, flavonoids, volatile components and other main antiviral and antibacterial components. Wherein the phillyrin has strong anti-inflammatory and antiviral effects as the main effective component of the forsythia suspense medicinal material.
The livestock and poultry viral diseases are infectious diseases which seriously jeopardize the production of the animal husbandry, can cause death of a large number of livestock and poultry and quality reduction of livestock products, influence the economic income and external trade of the livestock and poultry, and also bring serious threats to the health of people due to the co-morbidity of certain people and livestock. Livestock and poultry breeding plays a very important role in the breeding industry of China, and in the process of breeding livestock and poultry, due to weak resistance of the livestock and poultry, a series of stress factors generated by the change of the breeding environment and the like, viral diseases can often occur, and great threat is brought to the health of the livestock, poultry and people.
The Chinese veterinarian can enhance the prevention and treatment effect of the viral diseases of the livestock and poultry. Syndrome differentiation is the basic concept of Chinese veterinarian, and compared with Western medicine, it generally uses the treatment principles of strengthening body resistance and consolidating constitution and strengthening body resistance to eliminate pathogenic factors. In order to improve the immunity of livestock and poultry and treat the livestock and poultry with low immunity, a method of strengthening the body resistance is adopted; the application of the traditional Chinese medicine composition in treating livestock and poultry with healthy qi not deficient but excessive pathogenic factors is a method for eliminating pathogenic factors, so that the aim of balancing yin and yang can be fulfilled, and the effects of strengthening healthy qi and removing pathogenic factors are finally achieved. In general, Chinese veterinarians can use Chinese herbal medicines and acupuncture and moxibustion therapy methods, and the two methods can treat diseases of livestock and poultry and radically improve the physique of the livestock and poultry.
The Chinese veterinary medicine can prevent and treat diseases without toxic and side effects. The reasons for the infection of livestock and poultry by viral diseases are various, and the autoimmune systems of the livestock and poultry are complex and troublesome. Therefore, the immune promoting drugs and the inhibiting drugs in the western medicine treatment method cannot generate obvious treatment effect on the livestock and poultry invaded by the virus. In addition to poor therapeutic effect, toxic and side effects generated by western veterinary medicines can harm the health of livestock and poultry and edible people, and further can have negative effects on long-term development of livestock and poultry industry. In contrast, the immune promoting agent, the immune suppressing agent and the regulator suitable for livestock and poultry, which are used by Chinese veterinarians in the aspect of preventing and treating the viral diseases of livestock and poultry, can regulate and treat the organisms of the livestock and poultry to the maximum extent. It is worth mentioning that most of the medicines used by Chinese veterinarians for treating livestock and poultry before are natural component medicines with no toxic and side effects on the organisms of livestock and poultry, such as fructus forsythiae, liquorice, astragalus mongholicus and the like. From the practical point of view, one of the important obstacles affecting the development of animal husbandry is the problem of veterinary drug residues. The experts concerned believe that the effect of the ingredients of the veterinary drug itself will gradually replace some of the chemical drugs in western medicine, since it will not threaten the food safety of people.
The Chinese veterinarian adjusts the immunity function of the livestock body. In the field of Chinese veterinarian, the whole concept and the dialectical treatment principle are emphasized, and vital qi is the functions of viscera, defensive qi, nutrient blood and channels and collaterals of livestock and poultry, so that a defense system for preventing and controlling virus and bacteria invasion is avoided, external virus invasion is resisted, and the balance of yin and yang is adjusted. The modern immune system considers that the epidemic prevention system not only can play the roles of defense, monitoring and self-stabilization, but also can prevent the generation of pathogenic bacteria, which is similar to the concept of healthy qi resisting exogenous pathogenic factors. Through the function of oneself stabilizing, can clear away the internal source of illness of beasts and birds, this is all vital and vital to regulate yin, yang unbalance and similar. Because of the similarity between the concept of healthy qi and pathogenic qi and the immune system, the medicine also provides a basis for the veterinary medicine to treat viral diseases.
Improving the prevention and treatment effect of the viral diseases of the livestock and the poultry. The principle of traditional veterinarians lies in treating both principal and secondary aspects, and the view points are as follows: strengthen the body resistance to consolidate the constitution and eliminate pathogenic factors. The livestock and poultry with insufficient immunity can be treated in a body resistance strengthening way, so that the organism immunity of the livestock and poultry is improved, and the effect of disease-resistant treatment is realized. For the patients without deficiency of body resistance and excess of pathogenic factors, the body resistance can be strengthened and the pathogenic factors can be eliminated to realize the balance of the body. If the body resistance is deficient, the excess of pathogenic factors can be treated by strengthening the body resistance. There are two main methods: acupuncture and moxibustion and Chinese herbal medicine have wide application range in China, and the prevention and treatment effect is improved to a certain extent.
The Chinese veterinary medicine has the advantages of preventing and treating the viral diseases of the livestock and the poultry. The immune system of livestock is particularly complex, if a virus inhibitor and an immune injection are simply used, the effect is limited, which is also the reason that the western medicine immunotherapy mode is not ideal enough, and even some medicaments have side effects and toxic effects. However, the traditional Chinese medicine composition is analyzed from the prevention and treatment angle of a Chinese veterinarian, not only can be used as an immune promoter and an inhibitor, but also can be used as an immune regulator, and some traditional Chinese medicine compositions can also realize bidirectional regulation effect and improve the immunity of livestock and poultry, such as coptis chinensis, oldenlandia diffusa and the like.
In earlier work of the inventor of the application, forsythia is researched as a medicament for preventing and treating livestock and poultry viral diseases, and although certain effect is achieved, the treatment effect still has a space for further improvement. The present invention has been made in view of the above-mentioned problems.
Disclosure of Invention
Based on the background technology, the technical problem to be solved by the invention is to provide the forsythia suspense probiotic fermentation product capable of improving the treatment of livestock and poultry viral diseases. In order to realize the purpose of the invention, the following technical scheme is adopted:
the invention relates to a forsythia suspense probiotic fermented product, which is characterized by being prepared by the following steps:
pulverizing dried fructus forsythiae, sieving, adding purified water and glucose into fructus forsythiae powder, heating for sterilization, inoculating probiotic bacteria into the suspension, and adding CO2Culturing under air flow, distilling under reduced pressure to obtain extract, and freeze drying to obtain probiotic fermented product of fructus forsythiae.
In a preferred embodiment of the invention, the probiotic is a combination of enterococcus faecalis and bacillus megaterium. The invention is beneficial to improving the content of the forsythin in the fermentation product by combining the enterococcus faecalis and the bacillus megatherium.
In a preferred embodiment of the invention, the ratio of the using amount of the enterococcus faecalis to the using amount of the Bacillus megaterium is 1-2: 1-2; preferably 1: 1.
In a preferred embodiment of the invention, the enterococcus faecalis is from the strain of CICC deposit No. 20395.
In a preferred embodiment of the invention, the bacillus megaterium is a strain from the CICC deposit No. 10024.
It should be noted that although the present invention is carried out by using a specific strain, those skilled in the art can expect that the content of forsythin in the fermented product can be increased by using other enterococcus faecalis and bacillus megaterium even if the same technical effect as the embodiment of the present invention can not be completely achieved, but the content is different only in the increased range at most.
In a preferred embodiment of the invention, the content of forsythin in the fermentation product is more than 3.23mg/g of freeze-dried fermentation product.
In a preferred embodiment of the invention, the fermentation product contains active enterococcus faecalis and Bacillus megaterium.
In a preferred embodiment of the invention, the suspension is inoculated with probiotics in an amount of 0.5-2 x 108one/mL.
The invention also relates to application of the forsythia suspense probiotic fermentation product in preparation of a medicament for preventing and treating livestock and poultry viral diseases.
In a preferred embodiment of the present invention, the livestock and poultry viral disease is infectious bronchitis.
Advantageous effects
According to the invention, the forsythia is fermented by adopting the specific probiotic combination, so that the content of the forsythin in the fermentation product can be effectively increased, the obtained forsythia probiotic fermentation product can obviously prevent and treat epidemic diseases, improve the body performance of livestock and poultry, increase the daily gain of the livestock and poultry, and improve the production capacity of the livestock and poultry.
Detailed Description
In order to further understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Unless otherwise specified, the reagents involved in the examples of the present invention are all commercially available products, and all of them are commercially available.
Example 1: preparation of fructus forsythiae probiotic fermented product
Taking dry fructus forsythiae medicinal material, crushing and sieving with a 50-mesh sieve, adding 100g of purified water and 2g of glucose into 10g of fructus forsythiae powder, and heating and sterilizing. Inoculating the extract suspension with probiotic bacteria 108Each mL (wherein 1 half each of enterococcus faecalis from strain having CICC deposit number of 20395 and 1 half each of Bacillus megaterium from strain having CICC deposit number of 10024) in CO2Standing and culturing at 37 deg.C under air flow for 4 days, distilling under reduced pressure to obtain extract, and freeze drying to obtain probiotic fermented product (containing active probiotic bacteria) of fructus forsythiae.
Adding 70% ethanol 6 times the weight of the lyophilized probiotic fermented product, fully stirring and extracting for 1h, centrifuging to obtain supernatant, and determining the content of phillyrin by HPLC.
As comparative examples, the same procedure as in the examples was followed, differing only in the type and amount of probiotic added. The content of forsythin therein was also determined according to the method of example 1. The results are shown in Table 1.
Table 1: content of fructus forsythiae saponin
From the above experimental results, it can be seen that enterococcus faecalis and bacillus megatherium both contribute to the increase of the content of the active ingredient, namely, phillygenin, in the forsythia suspensa probiotic fermented product, but the combination of the enterococcus faecalis and the bacillus megatherium is more conducive to the increase of the content of the active ingredient, namely, phillygenin, in the forsythia suspensa probiotic fermented product. The mechanism is not completely clear, presumably because enterococcus faecalis and bacillus megatherium are conducive to the fermentation of some of the components in forsythia suspense to form a metabolite of forsythin.
Example 2: pharmacodynamic experiment for preventing and treating viral diseases of livestock and poultry
In the experimental example, the performance of the fermentation products obtained in example 1 and the comparative example was tested by using a chicken with infectious bronchitis as an experimental subject, and the specific scheme is as follows:
test animal
The 30-day-old black-bone chicken in the company laboratory has been diagnosed to have the infectious bronchitis by means of clinical symptom diagnosis, laboratory diagnosis and the like. 200 chickens are randomly selected from sick chicken flocks, and the chickens have both male and female, are divided into 4 groups with 50 chickens in each group, and have almost the same individual weight and mental state.
Method of administration
The negative control group uses the commercial medium-sized and large-sized chicken feed, and is fed 6 times a day with free feeding and free drinking; the test group adds the fermentation product obtained in the embodiment 1 of the invention into the feed according to 0.1 percent of the weight of the feed which is fed to the commercial middle and large chickens, and the mixture is mixed evenly; positive control group 1 and positive control group 2 the fermented product obtained in comparative example 3 and a commercial forsythin standard substance were added to the feed in an amount of 0.1% by weight of the commercial medium-sized chicken feed, respectively, and mixed uniformly. All experimental groups and positive control groups are fed according to a control formula method, namely feeding is carried out 6 times a day, free ingestion is carried out, drinking water is freely carried out, feeding is carried out, and feeding is carried out continuously for 2 months.
Results of the experiment
The cure rate, mortality rate, daily average weight gain and feed conversion ratio of each group of chickens were recorded. The feather color, appetite and mental status of the chickens in each group were observed, and the experimental results are shown in table 2.
Table 2: animal test results of fermented product
| Group of | Cure rate (%) | Mortality (%) | Daily average weight gain (g) | Meat ratio of materials |
| Negative control group | 42 | 58 | 75±6 | 4.5:1 |
| Positive control group 1 | 78 | 22 | 85±14 | 3.6:1 |
| Positive control group 2 | 84 | 16 | 100±15 | 3.2:1 |
| Test group | 92 | 8 | 101±12 | 2.8:1 |
As can be seen from Table 2, the effect difference between the test group and the control group in the aspect of treating the infectious bronchitis of chicken is very obvious (P is less than 0.01), and the cure rate of the fermentation product of the invention to the infectious bronchitis of chicken is more than 92%; the test group has quick recovery, good appetite and large feed intake, has good promotion effect on recovery of sick chickens after recovery, and the cured chicken feather is fresh, bright, red and moist, sleepy and less in movement; the daily average weight gain of the test group and the control group can reach 26g to the maximum, the improvement rate is 25 percent, and the difference is very obvious.
In addition, compared with a control group, the cure rate and the daily average weight gain of the test group are obviously improved, which shows that compared with the control group, the test group can obviously prevent and treat infectious bronchitis, improve the body performance of livestock and poultry, improve the daily average weight gain of the livestock and poultry and improve the production capacity of the livestock and poultry. The fermented product of the present invention has a higher cure rate even compared to the forsythin standard, presumably because the fermented product of the present invention further contains active probiotics, which act synergistically with the forsythin fermented product to improve the therapeutic effect
The foregoing describes preferred embodiments of the present invention, but is not intended to limit the invention thereto. Modifications and variations of the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.
Claims (10)
1. The forsythia suspense probiotic fermentation product is characterized by being obtained through the following steps:
pulverizing dried fructus forsythiae, sieving, adding purified water and glucose into fructus forsythiae powder, heating for sterilization, inoculating probiotic bacteria into the suspension, and adding CO2Culturing under air flow, distilling under reduced pressure to obtain extract, and freeze drying to obtain probiotic fermented product of fructus forsythiae.
2. A forsythia probiotic ferment according to claim 2, said probiotic being a combination of enterococcus faecalis and bacillus megaterium.
3. The forsythia suspense probiotic fermented product according to claim 2, wherein the ratio of the using amount of enterococcus faecalis to the using amount of bacillus megaterium is 1-2: 1-2; preferably 1: 1.
4. The forsythia probiotic ferment of claim 2, wherein said enterococcus faecalis is a strain from the CICC deposit No. 20395.
5. A forsythia probiotic fermentation according to claim 2, wherein the bacillus megaterium is a strain from the CICC deposit No. 10024.
6. The forsythia suspense probiotic ferment according to any one of claims 1 to 5, wherein the content of forsythin in the ferment is more than 3.23mg/g of freeze-dried ferment.
7. The forsythia suspense probiotic fermented product according to claim 6, which contains active enterococcus faecalis and bacillus megaterium.
8. A fermented product of probiotic bacteria of forsythia according to any one of claims 1 to 5, wherein the amount of probiotic bacteria inoculated to the suspension is 0.5 to 2 x 108one/mL.
9. Use of the forsythia suspensa probiotic fermentation product of the haloxylon ammodendron according to any one of claims 1 to 8 in the preparation of a medicament for preventing and treating viral diseases of livestock and poultry.
10. The use of claim 9, wherein the livestock and poultry viral disease is infectious bronchitis.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101874812A (en) * | 2010-05-14 | 2010-11-03 | 宁波市特圣生物饲料有限公司 | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea |
| CN103610759A (en) * | 2013-12-11 | 2014-03-05 | 吉林农业大学 | Mushroom and fructus forsythiae mixed fermentation product for preventing chicken coccocidiosis and preparation method thereof |
-
2021
- 2021-06-25 CN CN202110709589.2A patent/CN113368155A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101874812A (en) * | 2010-05-14 | 2010-11-03 | 宁波市特圣生物饲料有限公司 | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea |
| CN103610759A (en) * | 2013-12-11 | 2014-03-05 | 吉林农业大学 | Mushroom and fructus forsythiae mixed fermentation product for preventing chicken coccocidiosis and preparation method thereof |
Non-Patent Citations (1)
| Title |
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| 张羽璐等: "连翘酯苷对工感染传染性支气管炎病毒雏鸡肺组织JAK/STAT信号通路的调节作用", 《北京农学院学报》 * |
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