CN109646546B

CN109646546B - A Chinese medicinal composition for preventing and treating diarrhea of livestock, and its preparation method

Info

Publication number: CN109646546B
Application number: CN201910160457.1A
Authority: CN
Inventors: 方磊涵; 王振; 毕玉霞; 贾梧松; 贾宝生
Original assignee: Henan Traditional Chinese Veterinary Drug Research Institute Co ltd
Current assignee: Henan Traditional Chinese Veterinary Drug Research Institute Co ltd
Priority date: 2019-03-04
Filing date: 2019-03-04
Publication date: 2022-03-01
Anticipated expiration: 2039-03-04
Also published as: CN109646546A

Abstract

The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for preventing and treating livestock diarrhea and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: tea, common andrographis herb, barbed skullcap herb, common selfheal fruit-spike, golden thread, black nightshade leaf, dried rehmannia root, myrobalan fruit meat, carbonized sanguisorba root, dark plum fruit, cassia twig, fortune eupatorium herb, betel nut, fiveleaf gynostemma herb, dahurian patrinia herb and dahurian angelica root. The traditional Chinese medicine composition is prepared by fermenting according to a certain weight ratio according to a formula of Chinese veterinarian theory and is used for preventing and treating the livestock diarrhea caused by various reasons.

Description

A Chinese medicinal composition for preventing and treating diarrhea of livestock, and its preparation method

Technical Field

The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for preventing and treating livestock diarrhea and a preparation method thereof.

Background

Diarrhea has been a very troublesome problem in the swine industry and has been a bottleneck limiting the development of the swine industry. Diarrhea in piglets can lead to growth retardation, even growth retardation, loss of weight in adult pigs and reduced feed return rate. The morbidity is up to more than 80%, the mortality is 50-80%, the prevalence of the disease is very wide, and the large-scale pig farm almost has the prevalence of the disease, so that great economic loss is caused to the pig raising industry. With the intensive development of the pig industry, the pig diarrhea is increasingly high, which becomes an important problem restricting the development of the pig industry.

Diarrhea is commonly called diarrhea, is also the most common disease in cultivation, is caused by various pathogenic causes, is common as many as dozens of diseases, and has the largest loss of viral diarrhea to the cultivation industry in diarrhea caused by all pathogens. Epidemic porcine diarrhea (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus (PoRV) infection are the most common of diarrhea occurring, and are extremely harmful. In addition, bacterial diarrhea such as pig gastroenteritis, pig colibacillosis, pig paratyphoid fever, pig dysentery, and pig clostridial enteritis is also common. Clinically, mixed infection is often present. At present, no effective drug treatment is available for the diarrhea of pigs, the treatment effect of antibiotics with temporary and permanent treatment is not obvious, the drug resistance and drug residues are easy to generate, and from the current conditions of domestic livestock breeding environment, mode and technical level, livestock almost generate drug resistance to all antibiotics, thereby bringing hidden troubles to food safety and human health. Because of the numerous serotypes of the disease and different dominant serogroups in different regions, the traditional vaccine has poor protective effect and high cost no matter the vaccine is a weak vaccine or a inactivated vaccine. Therefore, the pure Chinese medicinal compound preparation for treating the porcine diarrhea, which has the advantages of definite curative effect, safety, reliability, small toxic and side effect and low cost, is provided for clinical medication, has unique curative effect and has important significance.

Disclosure of Invention

In view of the above, the invention provides a traditional Chinese medicine composition for preventing and treating livestock diarrhea and a preparation method thereof. The Chinese medicinal composition can be used for preventing and treating diarrhea of livestock such as pig.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a traditional Chinese medicine composition which is prepared from the following raw materials in parts by weight:

100-500 parts of tea, 100-300 parts of common andrographis herb, 100-200 parts of barbed skullcap herb, 100-200 parts of selfheal, 100-200 parts of coptis root, 100-200 parts of black nightshade leaf, 100-300 parts of dried rehmannia root, 100-300 parts of myrobalan meat, 50-200 parts of garden burnet root charcoal, 100-200 parts of dark plum fruit, 50-100 parts of cassia twig, 50-200 parts of fortune eupatorium herb, 30-200 parts of betel nut, 100-300 parts of fiveleaf gynostemma herb, 50-200 parts of dahurian patrinia herb and 50-200 parts of dahurian angelica root.

The traditional Chinese medicine composition is prepared by fermenting according to a certain weight ratio according to a Chinese veterinary theory formula and is used for preventing and treating piglet diarrhea caused by various reasons, wherein the traditional Chinese medicine composition treats both symptoms and root causes, treats both deficiency and excess, screens natural botanical medicines with the functions of clearing heat and removing toxicity, cooling blood and stopping bleeding, nourishing yin and promoting the production of body fluid, clearing heat and drying dampness, relieving diarrhea with astringents, promoting qi circulation and removing blood stasis, promoting digestion and removing food retention, strengthening body resistance and eliminating evil and improving the organism immune function.

The tea and the common andrographis herb are bitter in taste, cold and cool in nature, and are good at entering heart, lung, stomach and large intestine meridians for a large amount. The tea has the functions of clearing away heat and toxic material, promoting urination, helping digestion, regulating vital energy, reducing phlegm, relieving restlessness, quenching thirst, refreshing mind and astringing, and the common andrographis herb has the functions of clearing away heat and toxic material, cooling blood, reducing swelling and relieving pain and is mainly used for treating yellow diarrhea, red and white dysentery and the like; the barbed skullcap herb, the selfheal, the coptis root and the solanum nigrum leaf are bitter and cold and are mutually reinforced and used together to clear heat, dry dampness, cool blood, stop bleeding, clear heat, detoxify and purge fire; the rehmannia root has the effects of nourishing yin and promoting the production of body fluid, can clear heat in the nutrient system and has the effects of cooling blood and stopping bleeding, and the tea and the common andrographis herb can clear damp-heat in the stomach and intestine to stop dysentery; myrobalan, charred sanguisorba and dark plum are sour in taste, have the effects of relieving diarrhea with astringents and astringing lung to relieve asthma, and are matched with coptis chinensis to be good at treating chronic diarrhea and chronic dysentery; ramulus Cinnamomi and herba Eupatorii are pungent and warm in nature, and have effects of activating yang, regulating qi-flowing, warming channels, dredging collaterals, activating qi-flowing, removing blood stasis, invigorating stomach, and eliminating dampness. The betel nut is bitter, pungent and warm in nature, has the effects of promoting the circulation of qi in the stomach and intestine, removing food retention and removing food stagnation, invigorating stomach and promoting appetite, relieving pain and regulating the middle warmer, dissipating blood stasis and promoting qi circulation, and can enhance the effects of relaxing bowels and relieving diarrhea; gynostemma pentaphylla and herba patriniae are bitter in taste and cool in nature, enter yangming meridians, clear away heat and toxic materials, strengthen body resistance to eliminate pathogenic factors, enhance immune function and recover by using the organism. Bai Zhi is pungent and damp, very fragrant, and can dry dampness, so it is the channel-guiding herb for Yangming channel. The medicines are combined, thereby clearing away heat and toxic material, relieving diarrhea with astringents and curing various diseases.

The pharmacological action of the traditional Chinese medicine compound is as follows:

1. the function of resisting pathogenic microorganisms: the preparation has broad-spectrum antibacterial and antiviral effects. In the formula, the decoction of tea, barbed skullcap herb, common andrographis herb, common selfheal fruit-spike, dark plum fruit, golden thread, cassia twig, fortune eupatorium herb and the like has stronger inhibiting effect on escherichia coli, dysentery bacillus, pseudomonas aeruginosa, vibrio cholerae, staphylococcus aureus, diphtheria bacillus, pneumococcus, streptococcus, proteus, typhoid bacillus, paratyphoid bacillus, tubercle bacillus and the like and part of pathogenic fungi. The medicine also has obvious antiviral effect, and has obvious inhibiting effect on infectious gastroenteritis virus, rotavirus, coronavirus, hog cholera virus, parvovirus, porcine pseudocanine virus, porcine reproductive and respiratory syndrome virus, influenza virus and the like.

2. Immune enhancement effect: the Chinese medicinal compound has the effect of enhancing organism immunity, and is an immunity-stimulating natural Chinese herbal medicine. In the formula, the common andrographis herb, the coptis chinensis, the common selfheal fruit-spike, the gynostemma pentaphylla, the barbed skullcap herb, the dahurian patrinia herb and the like can obviously promote an organism to generate antibodies, enhance the phagocytic function of macrophages, promote lymphocyte transformation, improve the activity of white blood cells in the organism, improve the weight, the spleen index and the spleen lymphocyte transformation stimulation index of an immunosuppression mouse, and improve the levels of IgG, IgA and IgM of the organism and complements C3 and C4 in serum.

3. Modulation of central nerves: in the formula, the myrobalan meat, the angelica dahurica and the radix rehmanniae have the functions of detoxifying, easing pain, relieving spasm, relieving intestinal peristalsis and accelerating to achieve the aim of relieving diarrhea, and also have the functions of resisting inflammation, bacteria, viruses, allergy and heart. For example, the dried rehmannia root extract has a remarkable enhancing effect on the contractility of frog heart, is more remarkable for weak heart, and can enhance the reserve of coronary artery.

4. The exudation of the wound surface is reduced: in the formula, the carbonized sanguisorba root and the black nightshade leaf have the effects of reducing wound exudation, astringing and stopping bleeding. The radix Sangusorbae charcoal can inhibit various pathogenic microorganisms and tumors, and folium Solani Nigri can reduce vascular permeability and activity of hyaluronidase, has strong anti-inflammatory effect, and can promote antibody formation.

5. In the formula, areca catechu contains arecoline, which is an effective insect repellent ingredient. The decoction and water extract have strong inhibitory effect on influenza virus, and can promote secretion of digestive juice and stimulate appetite. Has good effects on resisting pathogenic microorganisms and cancers.

The functions are as follows: clearing away heat and toxic materials, cooling blood, stopping bleeding, eliminating dampness, relieving diarrhea with astringents, promoting qi circulation, removing blood stasis, resolving food stagnation, killing parasite, killing bacteria, strengthening body resistance, eliminating pathogenic factors, nourishing yin, promoting fluid production, and promoting immunity.

The main treatment is as follows: diarrhea due to yellow intestine and damp-heat, and can be used for treating pig intestinal diseases. Has the functions of preventing and treating diarrhea of single pigs or pigs with mixed infection, such as bacteria, virus, parasites, and the like, and has ideal effects of preventing and treating the diarrhea for the growth of piglets and improving the feed reward. Has the effects of appetizing and promoting growth of cad pigs which are resistant to over survival and grow slowly.

Preferably, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:

300 parts of tea, 200 parts of common andrographis herb, 150 parts of barbed skullcap herb, 150 parts of common selfheal fruit-spike, 150 parts of golden thread, 150 parts of black nightshade leaf, 200 parts of dried rehmannia root, 200 parts of myrobalan meat, 150 parts of carbonized sanguisorba root, 150 parts of dark plum fruit, 75 parts of cassia twig, 150 parts of fortune eupatorium herb, 150 parts of areca seed, 200 parts of fiveleaf gynostemma herb, 150 parts of dahurian patrinia herb and 150 parts of dahurian angelica root.

100 parts of tea leaves, 300 parts of common andrographis herbs, 100 parts of barbed skullcap herbs, 200 parts of common selfheal fruits, 100 parts of coptis roots, 200 parts of black nightshade leaves, 100 parts of dried rehmannia roots, 300 parts of myrobalan pulp, 50 parts of carbonized sanguisorba roots, 200 parts of dark plums, 50 parts of cassia twigs, 200 parts of fortune eupatorium herbs, 30 parts of betel nuts, 300 parts of fiveleaf gynostemma herbs, 50 parts of dahurian patrinia herbs and 200 parts of dahurian angelica roots.

500 parts of tea, 100 parts of common andrographis herb, 200 parts of barbed skullcap herb, 100 parts of common selfheal fruit-spike, 200 parts of golden thread, 100 parts of black nightshade leaf, 300 parts of dried rehmannia root, 100 parts of myrobalan meat, 200 parts of carbonized sanguisorba root, 100 parts of dark plum fruit, 100 parts of cassia twig, 50 parts of fortune eupatorium herb, 200 parts of areca seed, 100 parts of fiveleaf gynostemma herb, 200 parts of dahurian patrinia herb and 50 parts of dahurian angelica root.

The invention also provides application of the traditional Chinese medicine composition in preparing a medicine for preventing or treating livestock diarrhea.

In the present invention, the livestock is pigs.

The invention also provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:

drying and crushing the raw materials to obtain traditional Chinese medicine powder;

adding composite probiotics into the traditional Chinese medicine powder for fermentation;

drying the fermentation product to obtain the Chinese medicinal composition.

Preferably, the composite probiotics consist of lactobacillus, bacillus subtilis and yeast.

Preferably, the ratio of the number of live bacteria of lactobacillus, bacillus subtilis and yeast is (1-10): (1-10): (1-10).

Preferably, the ratio of the number of live bacteria of lactobacillus, bacillus subtilis and yeast is 1:1: 1.

preferably, the adding amount of the composite probiotics in the traditional Chinese medicine powder is 10-15% by mass percentage.

Preferably, the adding amount of the composite probiotics in the traditional Chinese medicine powder is 12% by mass percentage.

Preferably, the fermentation temperature is 30-37 ℃, the fermentation humidity is 70-80%, and the fermentation time is 40-60 h.

Preferably, the fermentation temperature is 30-37 ℃, the fermentation humidity is 70-80%, and the fermentation time is 48 h.

Preferably, the fineness of the pulverized material is 50 to 100 mesh.

Preferably, the fineness of pulverization is 80 mesh.

Preferably, the temperature for drying the fermentation product is 60-80 ℃.

Preferably, the temperature at which the fermentation product is dried is 70 ℃.

The invention provides a traditional Chinese medicine composition for preventing and treating livestock diarrhea and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 100-500 parts of tea, 100-300 parts of common andrographis herb, 100-200 parts of barbed skullcap herb, 100-200 parts of selfheal, 100-200 parts of coptis root, 100-200 parts of black nightshade leaf, 100-300 parts of dried rehmannia root, 100-300 parts of myrobalan meat, 50-200 parts of garden burnet root charcoal, 100-200 parts of dark plum fruit, 50-100 parts of cassia twig, 50-200 parts of fortune eupatorium herb, 30-200 parts of betel nut, 100-300 parts of fiveleaf gynostemma herb, 50-200 parts of dahurian patrinia herb and 50-200 parts of dahurian angelica root. The traditional Chinese medicine composition has the following advantages:

firstly, the effective components and active substances of the medicine can be extracted and utilized to the maximum extent;

secondly, the effective active components which can not be directly utilized after the medicine enters the body are directly utilized because the medicine is finished in vitro, and the due efficacy is quickly exerted;

the preferable beneficial bacteria of the organism have the function of supplementing or enhancing the original medicine;

compared with the original medicine, the traditional Chinese medicine fermentation pharmacy of the invention generates new active substances, thereby having new health care, prevention or treatment functions;

the invention is a new technology with high technology level for realizing the modernization of traditional Chinese medicine, the production process is controllable, the obtained product is accurate, the preparation is convenient, and the international connection is convenient.

Detailed Description

The invention discloses a traditional Chinese medicine composition for preventing and treating livestock diarrhea and a preparation method thereof, and a person skilled in the art can use the contents for reference and appropriately improve process parameters for realization. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.

The traditional Chinese medicine composition is prepared by taking the theory of traditional Chinese medicines as guidance and taking tea leaves, common andrographis herbs, barbed skullcap herbs, common selfheal fruit-spike and the like as main raw materials. The results of the previous studies show that: the medicine has stronger antiviral, antibacterial, anti-inflammatory, astringent, antidiarrheal and immunity-improving effects, and has good treatment effect on livestock diarrhea.

The traditional Chinese medicine composition for preventing and treating livestock diarrhea and the preparation method thereof provided by the invention can be purchased from the market as raw materials or auxiliary materials. The lactobacillus is lactobacillus of mixed medicament of lactobacillus plantarum and lactobacillus acidophilus (viable count ratio is 1:1), the bacillus is bacillus subtilis, and the yeast is Angel yeast as yeast in the leavening agent.

The invention is further illustrated by the following examples:

example 1

1. The formula of the traditional Chinese medicine preparation is as follows:

300g of tea, 200g of common andrographis herb, 150g of barbed skullcap herb, 150g of common selfheal fruit-spike, 150g of golden thread, 150g of black nightshade leaf, 200g of dried rehmannia root, 200g of myrobalan meat, 150g of carbonized sanguisorba root, 150g of dark plum fruit, 75g of cassia twig, 150g of fortune eupatorium herb, 150g of areca nut, 200g of fiveleaf gynostemma herb, 150g of dahurian patrinia herb and 150g of dahurian angelica root.

2. The preparation method of the Chinese medicinal preparation comprises the following steps:

the compound traditional Chinese medicine consisting of the 16 Chinese medicines is dried, crushed and sieved by a 80-mesh sieve. Activating and counting lactobacillus, bacillus subtilis and saccharomycetes, inoculating the counted probiotics (the total inoculation amount is 12 percent according to the proportion of 1:1: 1) into the crushed compound traditional Chinese medicine, and putting the mixture into a fermentation tank or a fermentation pool for fermentation for 48 hours (the temperature is 30-37 ℃, and the humidity is 70-80%). Drying the fermented product (the temperature is controlled at 70 ℃), and obtaining the fermented traditional Chinese medicine preparation.

Example 2

1. The formula of the traditional Chinese medicine preparation is as follows:

100g of tea leaves, 300g of common andrographis herb, 100g of barbed skullcap herb, 200g of common selfheal fruit-spike, 100g of golden thread, 200g of black nightshade leaf, 100g of dried rehmannia root, 300g of myrobalan meat, 50g of carbonized sanguisorba root, 200g of dark plum fruit, 50g of cassia twig, 200g of fortune eupatorium herb, 30g of areca-nut, 300g of fiveleaf gynostemma herb, 50g of dahurian patrinia herb and 200g of dahurian angelica root.

the compound traditional Chinese medicine consisting of the 16 Chinese medicines is dried, crushed and sieved by a 80-mesh sieve. Activating and counting lactobacillus, bacillus subtilis and saccharomycetes, inoculating the counted probiotics (the total inoculation amount is 12 percent according to the proportion of 1:1: 1) into the crushed compound traditional Chinese medicine, and putting the mixture into a fermentation tank or a fermentation pool for fermentation for 48 hours (the temperature is 30-37 ℃, and the humidity is 70-80%). Drying the fermentation product (the temperature is controlled at 60 ℃) to obtain the fermented traditional Chinese medicine preparation.

Example 3

1. The formula of the traditional Chinese medicine preparation is as follows:

500g of tea leaves, 100g of common andrographis herb, 200g of barbed skullcap herb, 100g of common selfheal fruit-spike, 200g of golden thread, 100g of black nightshade leaf, 300g of dried rehmannia root, 100g of myrobalan meat, 200g of carbonized sanguisorba root, 100g of dark plum fruit, 100g of cassia twig, 50g of fortune eupatorium herb, 200g of areca-nut, 100g of fiveleaf gynostemma herb, 200g of dahurian patrinia herb and 50g of dahurian angelica root.

the compound traditional Chinese medicine consisting of the 16 Chinese medicines is dried, crushed and sieved by a 80-mesh sieve. Activating and counting lactobacillus, bacillus subtilis and saccharomycetes, inoculating the counted probiotics (the total inoculation amount is 12 percent according to the proportion of 1:1: 1) into the crushed compound traditional Chinese medicine, and putting the mixture into a fermentation tank or a fermentation pool for fermentation for 48 hours (the temperature is 30-37 ℃, and the humidity is 70-80%). Drying the fermentation product (the temperature is controlled at 80 ℃) to obtain the fermented traditional Chinese medicine preparation.

Test example 1 clinical therapeutic effect

1. Clinical symptoms:

the diarrhea latency of the pig is short, the disease is fast, and the death rate is high. The main clinical symptoms are the cause of vomiting and diarrhea to varying degrees. Sows, breeding boars and fattening pigs are frequently infected once and recover about 1 week with slight symptoms; piglets show vomiting, diarrhea, severe watery diarrhea in jet form, severe bloody stool, listlessness, anorexia, rough and disorganized fur, loose anus, continuous yellow-green or grey-white watery or egg flower-like feces discharge, are rapidly dehydrated and emaciated, die after failing within 1 week, the body temperature can reach more than 39 ℃, and the cured piglets have poor growth and development.

2. Pathological changes are as follows:

the cesarean examination of the dead piglet is changed into the symptoms of small intestinal tube fullness and flatulence, thinned and bright intestinal wall, liquid or paste contents with different colors in the intestine (TGEV and PEDV are yellow green or grey white, and PoRV is dark black or yellow white), lymph node swelling, milk clotting in the stomach, congestion of partial gastric mucosa and small intestinal mucosa, bleeding and swelling. The histological changes mainly include atrophy and shortening of intestinal villi, even shedding of intestinal villi and vacuolization of intestinal villus epithelial cells.

3. Laboratory examination:

the viral diarrhea is determined by performing amplification detection on feces of piglets with diarrhea, intestinal contents of pigs died of diseases and mesenteric lymph nodes by adopting an RT-PCR method. The diarrhea of the bacteria is prepared by inoculating a disease material on a blood plate culture medium according to a conventional streak bacteria separation method, culturing at 37 ℃ for 14h, selecting a single colony for purification culture, performing biochemical identification, and performing a glass plate agglutination test by using Escherichia coli O-type serum and salmonella serum to identify the serotype of the separated bacteria.

4. The treatment method comprises the following steps:

prevention amount: 2 kg of the traditional Chinese medicine compound leaven is mixed with 1 ton of feed for feeding.

② the treatment amount: 500g of the Chinese herbal compound starter is mixed with 100 kg of feed to feed, and the mixture is fed for 7 days, and 1% of Chinese herbal compound starter water (5 g of the Chinese herbal compound starter is dissolved in 1 jin of water) needs to be drunk in a matching way during the treatment period.

In the treatment period, the traditional Chinese medicine compound leaven can be applied in an appropriate amount to the case with serious disease nature, and the pigs with no appetite can be drenched by people according to 0.5 g/kg of body weight, and oral tonic liquid (formula: 3.5 g of salt, 20 g of glucose, 2.5 g of baking soda, l.5 g of potassium chloride and 1000 g of water) is matched to improve the cure rate.

5. Grouping tests:

the RT-PCR method is adopted to detect the genes of the porcine epidemic diarrhea, the porcine transmissible gastroenteritis and the porcine rotavirus which are treated by the outpatient service and are determined to be positive. The etiology of bacterial diarrhea is determined by isolated culture of bacteria and serotype identification.

According to the compatibility principle of the traditional Chinese medicines, the traditional Chinese medicine formula disclosed by the invention is combined into two compound formulas, the original formula is quantitatively changed, the traditional Chinese medicine formula and the compound formulas disclosed by the invention are compared and tested with clinical treatment cases, the test method divides sick pigs with viral diarrhea and bacterial diarrhea into 8 groups, and the conditions of the medicines in each group are as follows:

group one (traditional Chinese medicine test group 1): is the traditional Chinese medicine of the embodiment 1 of the invention.

Second group (Chinese medicine experiment 2 group): is the traditional Chinese medicine of the embodiment 2 of the invention.

Third group (traditional Chinese medicine experiment 3 groups): is the traditional Chinese medicine of the embodiment 3 of the invention.

Fourth group (traditional Chinese medicine control 1 group): 300g of tea, 150g of selfheal, 150g of coptis, 200g of radix rehmanniae, 200g of myrobalan meat, 150g of fortune eupatorium herb, 200g of gynostemma pentaphylla, 150g of angelica dahurica and 150g of carbonized sanguisorba root.

Group five (traditional Chinese medicine control group 2): 200g of common andrographis herb, 200g of dahurian patrinia herb, 200g of myrobalan meat, 150g of barbed skullcap herb, 150g of dark plum fruit, 150g of fortune eupatorium herb, 150g of betel nut, 150g of golden thread, 150g of black nightshade leaf and 75g of cassia twig.

Group six (traditional Chinese medicine control group 3): 150g of tea leaves, 100g of common andrographis herb, 100g of barbed skullcap herb, 100g of common selfheal fruit-spike, 100g of golden thread, 100g of black nightshade leaf, 100g of dried rehmannia root, 100g of myrobalan meat, 100g of carbonized sanguisorba root, 100g of dark plum fruit, 50g of cassia twig, 100g of fortune eupatorium herb, 100g of areca nut, 100g of fiveleaf gynostemma herb, 100g of dahurian patrinia herb and 100g of dahurian angelica root.

Group seven (western medicine control group): 0.75ml/kg of gentamicin sulfate, 0.75/kg of lincomycin hydrochloride and 0.15/kg of mequindox are injected intramuscularly.

Eighth group (CN102697888A example 1 chinese medicinal fermented preparation): 10 parts of common andrographis herb, 8 parts of pomegranate bark, 8 parts of breynia fruticosa, 8 parts of purslane, 6 parts of lightyellow sophora root, 10 parts of Chinese pulsatilla root and 10 parts of coptis chinensis.

6. Determination of therapeutic effect

The effect is shown: after the sick pigs are taken for 3 days, the mental state and the appetite are obviously improved, the excrement is recovered to be normal, and patients without obvious digestive tract lesions are healed by the autopsy;

the method has the following advantages: after the sick pigs are taken for 5 days, the mental state and the appetite are improved, the feces are obviously changed, and finally, the pigs are effective for the patients without death;

and (4) invalidation: after the tested piglets are administrated for 7 days, the symptoms are not obviously improved, even aggravated or dead, and the piglets with obvious digestive tract lesion characteristics in death and autopsy are invalid.

7. Test results

The test results are shown in tables 1 and 2.

TABLE 1 Effect of Chinese herbal compound for treating porcine viral diarrhea

The test results in table 1 show that the treatment effect of the traditional Chinese medicine compound group is obviously better than that of other groups. The traditional Chinese medicine composition has better curative effect on the porcine viral diarrhea.

TABLE 2 Effect of Chinese herbal compound for treating porcine bacterial diarrhea

Table 2 the test results show that: the traditional Chinese medicine compound group has a good treatment effect on piglet diarrhea caused by bacteria, and the effect is obviously better than that of other groups.

Test example 2 clinical cases

Case one:

in late 10 months in 2017, piglets in the whole lactation period of a certain pig farm in used as a personal name in ancient times yang district of Shangqiu city suddenly suffer from vomiting and explosive diarrhea (the morbidity is more than 80%), are yellow water-like feces with fishy smell and automatically flow along anus, part of piglets are dehydrated and die, the body temperature is 39-39.5 ℃, and the attack of the piglets is concentrated between 1-10 days of birth in a delivery room. The pig died from the disease is examined by a dissecting way, the intestinal wall is thin and semitransparent, the gastrointestinal mucosa has bleeding, the kidney is swollen, and other organs have no obvious change. Laboratory examination shows that the pig farm is diarrhea caused by the combined infection of epidemic diarrhea virus and porcine rotavirus. After the formula is applied to treat 3-5 days, the appetite of 60% of sick pigs is recovered, diarrhea disappears, and the quality of excrement is normal. 20 percent of sick pigs have better spirit and appetite, the diarrhea frequency is obviously reduced, and on the basis of the treatment of the formula, 0.75ml/kg of gentamicin, 0.75ml/kg of lincomycin and 0.15ml/kg of mequindox are injected intramuscularly (basic veterinary custom application), and the pigs are cured after 7 days. All symptoms of 20% of sick pigs are not improved, and the disease condition is aggravated to death.

Case two:

in the middle of 2 months of 2017, 480 piglets are transplanted to a herd piglet at a certain pig farm of a populus town of Cai county in Ma shop for 52 days, and after 5 days after the herd transplantation, 341 piglets are suffered from depression, anorexia, severe diarrhea at the body temperature of 39.8-41 ℃, yellow and thin water sample feces are discharged, congestion of intestinal mucosa is dissected and detected on dead pigs, intestinal lymph nodes are swollen, exudate in sections is increased, and the herd piglets are diarrhea caused by escherichia coli infection through biochemical identification and analysis of bacteria. The traditional Chinese medicine compound is used for treating for 3-5 days, 318 heads are cured, 20 heads are improved, and 3 heads are invalid. The traditional Chinese medicine compound has a good curative effect on piglet diarrhea caused by bacterial infection.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The traditional Chinese medicine composition is characterized by being prepared from the following raw materials in parts by weight:

100-500 parts of tea, 100-300 parts of common andrographis herb, 100-200 parts of barbed skullcap herb, 100-200 parts of selfheal, 100-200 parts of coptis root, 100-200 parts of black nightshade leaf, 100-300 parts of dried rehmannia root, 100-300 parts of myrobalan meat, 50-200 parts of garden burnet root charcoal, 100-200 parts of dark plum fruit, 50-100 parts of cassia twig, 50-200 parts of fortune eupatorium herb, 30-200 parts of betel nut, 100-300 parts of fiveleaf gynostemma herb, 50-200 parts of dahurian patrinia herb and 50-200 parts of dahurian angelica root;

the preparation method comprises the following steps: drying and crushing the raw materials to obtain traditional Chinese medicine powder; adding composite probiotics into the traditional Chinese medicine powder for fermentation; drying the fermentation product to obtain the traditional Chinese medicine composition; the composite probiotics comprise lactobacillus, bacillus subtilis and saccharomycetes, wherein the viable count ratio of the lactobacillus to the bacillus subtilis to the saccharomycetes is (1-10): (1-10): (1-10); the adding amount of the composite probiotics in the traditional Chinese medicine powder is 10-15% by mass percentage.

2. The traditional Chinese medicine composition according to claim 1, which is prepared from the following raw materials in parts by weight:

3. The traditional Chinese medicine composition according to claim 1, which is prepared from the following raw materials in parts by weight:

4. The traditional Chinese medicine composition according to claim 1, which is prepared from the following raw materials in parts by weight:

5. Use of the Chinese medicinal composition of any one of claims 1-4 in the preparation of a medicament for preventing or treating diarrhea in livestock.

6. Use according to claim 5, wherein the livestock animal is a pig.

7. The method for preparing a Chinese medicinal composition according to any one of claims 1 to 4, comprising the steps of:

drying the fermentation product to obtain the traditional Chinese medicine composition;

the composite probiotics comprise lactobacillus, bacillus subtilis and saccharomycetes, wherein the viable count ratio of the lactobacillus to the bacillus subtilis to the saccharomycetes is (1-10): (1-10): (1-10); the adding amount of the composite probiotics in the traditional Chinese medicine powder is 10-15% by mass percentage.

8. The preparation method according to claim 7, wherein the fermentation temperature is 30-37 ℃, the fermentation humidity is 70-80%, and the fermentation time is 40-60 h.

9. The preparation method according to claim 7 or 8, wherein the fineness of the pulverization is 50 to 100 mesh, and the temperature for drying the fermentation product is 60 to 80 ℃.