CN111067974A - Chinese herbal medicine preparation for preventing African swine fever virus and preparation method thereof - Google Patents
Chinese herbal medicine preparation for preventing African swine fever virus and preparation method thereof Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
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- A61K2236/10—Preparation or pretreatment of starting material
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention provides a Chinese herbal medicine preparation for preventing African swine fever virus and a preparation method thereof. The preparation method is simple and reasonable, and the prepared traditional Chinese medicine grass preparation has the advantages that the lipid bilayer of the virus envelope is damaged, so that the virus is unstable, the membrane is split, and finally the replication of the virus is inhibited, thereby playing the role of resisting the envelope virus; and can effectively resist, restrain and eliminate the infection and damage of the virus to the organism. Meanwhile, the feed additive also has the functions of supplementing beneficial microorganisms in the intestinal tract, improving the gastrointestinal function, inhibiting the growth of harmful bacteria, balancing the micro-ecological environment of the intestinal tract and improving the immunity of the organism. The invention adopts herbaceous plant extraction, avoids chemical formula addition, and has the advantages of safety, reliability, high utilization rate, low cost and no toxic or side effect.
Description
Technical Field
The invention belongs to the field of prevention of African swine fever, and particularly relates to a Chinese herbal medicine preparation for preventing African swine fever viruses and a preparation method thereof.
Background
African swine fever is an acute, hemorrhagic, highly contagious disease caused by African swine fever virus infecting domestic pigs and various wild pigs (African wild pigs, European wild pigs, etc.). Has the characteristics of short disease course, high mortality rate of the most acute and acute infections of 100 percent and the like; it often presents with clinical symptoms of anorexia, hyperpyrexia (40-42 ℃), leukopenia, bleeding of skin and internal organs, high mortality, etc.
China is a big country for raising pigs and pork consumption, the marketing amount, the stocking amount and the pork consumption amount of live pigs are all in the top of the world, the total import amount of the pigs and pork products is huge every year, the trade with a plurality of countries is frequent, and the number of commodities carried by passengers is large and the types are mixed. Therefore, the risk of transferring the African swine fever into China is increased, and once the African swine fever is transferred, the direct and indirect loss caused by the African swine fever is immeasurable. Therefore, how to effectively prevent and control African swine fever is a key problem to be urgently solved in the current pig industry.
Disclosure of Invention
The invention provides a Chinese herbal medicine preparation for preventing African swine fever virus and a preparation method thereof.
The technical scheme of the invention is realized as follows:
a Chinese herbal medicine preparation for preventing African swine fever virus comprises the following raw materials in percentage by weight:
10-12 parts of astragalus membranaceus, 10-12 parts of codonopsis pilosula, 10-12 parts of honeysuckle, 8-10 parts of angelica sinensis, 8-10 parts of dandelion, 8-10 parts of fructus forsythiae, 4-6 parts of liquorice, 6-8 parts of folium isatidis and 8-10 parts of rehmannia glutinosa.
Further, the material comprises the following raw materials in percentage by weight:
10 parts of astragalus, 12 parts of codonopsis pilosula, 11 parts of honeysuckle, 9 parts of angelica, 8 parts of dandelion, 10 parts of fructus forsythiae, 5 parts of liquorice, 7 parts of folium isatidis and 9 parts of rehmannia.
The invention also provides a preparation method of the Chinese herbal medicine preparation for preventing African swine fever virus, which comprises the following steps:
1) cleaning radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, Glycyrrhrizae radix, folium Isatidis, and rehmanniae radix, and oven drying;
2) weighing the raw materials in the step 1) in proportion, soaking the raw materials in ethanol for 30min, then carrying out ultrasonic treatment for 15min, carrying out reflux extraction for 2h for the first time, and filtering the extract by using sixteen layers of gauze to obtain a filtrate;
3) adding ethanol into the dregs obtained in the step 2), performing reflux extraction for the second time for 1 hour, filtering with sixteen layers of gauze to obtain filtrate, and combining the two filtrates to obtain a liquid medicine;
4) and (3) rotatably evaporating the liquid medicine obtained in the step 3) until no alcohol smell exists, adding water and white sugar into the concentrated solution, heating and boiling for 5min, cooling to room temperature, adding streptococcus faecalis, fermenting for 72h, and vacuum drying to obtain the Chinese herbal medicine preparation for preventing African swine fever viruses.
Further, in the step 2), the mass concentration of the ethanol is 80%, and the adding amount of the ethanol is 10 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the fructus forsythiae, the liquorice, the folium isatidis and the rehmannia.
Further, in the step 3), the mass concentration of the ethanol is 60%, and the adding amount of the ethanol is 8 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the fructus forsythiae, the liquorice, the folium isatidis and the rehmannia.
Further, in the step 4), the mass ratio of the volume of the water to the concentrated solution is 1: 1, the adding amount of the white sugar is 1.5 percent of the mass of the concentrated solution.
Further, in the step 4), the inoculation amount of the streptococcus faecalis is 3 multiplied by 106-3×107cfu/g。
Further, the temperature of vacuum drying was 37 ℃.
The invention provides a Chinese herbal medicine preparation for preventing African swine fever virus and a preparation method thereof, and the Chinese herbal medicine preparation has the following advantages:
1) the astragalus can obviously increase the total number of white blood cells in blood, promote the phagocytic function and the bactericidal capability of neutrophils and macrophages, obviously enhance cellular immunity and promote the lymphocyte transformation caused by phytohemagglutinin, concanavalin A, phytolaccin and the like; the codonopsis pilosula contains various chemical components such as saponin, cane sugar, starch, glucose, mucus, trace microbial alkali and the like, and has the effects of enriching blood, reducing blood pressure, tonifying middle-jiao and Qi and the like; the honeysuckle has the functions of resisting pathogenic microorganisms, resisting inflammation, detoxifying, regulating immunity, protecting liver, reducing blood fat and the like; the angelica can obviously enhance the phagocytic function of abdominal macrophage and the humoral immunity function of the organism, and the angelica polysaccharide has stronger activation effect on T lymphocyte, can obviously promote the proliferation of mouse lymphocyte, directly activates the T lymphocyte participating in antibody reaction, makes partial cell group become antibody secreting cell and has synergistic effect with the concanavalin. The reasonable compatibility of the raw materials can effectively enhance the immune function of the organism, improve the immunity of the organism and achieve the aim of preventing the African swine fever.
2) The preparation method is simple and reasonable, and the prepared traditional Chinese medicine grass preparation has the advantages that the lipid bilayer of the virus envelope is damaged, so that the virus is unstable, the membrane is split, and finally the replication of the virus is inhibited, thereby playing the role of resisting the envelope virus; and can effectively resist, restrain and eliminate the infection and damage of the virus to the organism. Meanwhile, the feed additive also has the functions of supplementing beneficial microorganisms in the intestinal tract, improving the gastrointestinal function, inhibiting the growth of harmful bacteria, balancing the micro-ecological environment of the intestinal tract and improving the immunity of the organism. The invention adopts herbaceous plant extraction, avoids chemical formula addition, and has the advantages of safety, reliability, high utilization rate, low cost and no toxic or side effect.
Detailed Description
The invention provides a Chinese herbal medicine preparation for preventing African swine fever virus, which comprises the following raw materials in percentage by weight:
10-12 parts of astragalus membranaceus, 10-12 parts of codonopsis pilosula, 10-12 parts of honeysuckle, 8-10 parts of angelica sinensis, 8-10 parts of dandelion, 8-10 parts of fructus forsythiae, 4-6 parts of liquorice, 6-8 parts of folium isatidis and 8-10 parts of rehmannia glutinosa.
The astragalus can obviously increase the total number of white blood cells in blood, promote the phagocytic function and the bactericidal capacity of neutrophils and macrophages, obviously enhance cellular immunity and promote the lymphocyte transformation caused by phytohemagglutinin, concanavalin A, phytolaccin and the like; the codonopsis pilosula contains various chemical components such as saponin, cane sugar, starch, glucose, mucus, trace microbial alkali and the like, and has the effects of enriching blood, reducing blood pressure, tonifying middle-jiao and Qi and the like; the honeysuckle has the functions of resisting pathogenic microorganisms, resisting inflammation, detoxifying, regulating immunity, protecting liver, reducing blood fat and the like; the angelica can obviously enhance the phagocytic function of abdominal macrophage and the humoral immunity function of the organism, and the angelica polysaccharide has stronger activation effect on T lymphocyte, can obviously promote the proliferation of mouse lymphocyte, directly activates the T lymphocyte participating in antibody reaction, makes partial cell group become antibody secreting cell and has synergistic effect with the concanavalin.
The traditional Chinese veterinarian considers that the onset transformation of the African swine fever in the pig body can be treated by dialectically treating the upper jiao, the middle jiao, the lower jiao and the defense, qi, nutrient and blood. The 'deficiency-evil coordination' is a cause of disease occurrence, namely that the five internal organs in the traditional Chinese medicine are in deficiency and the virus enters the five internal organs in deficiency, so that the five internal organs are in excess and weak, and the disease resistance is weak; the Chinese herbal medicines can harmonize yin and yang, treat both principal and secondary aspects of diseases, enable the organism to be as same as the body in physique and immunity, and balance yin and yang, thereby achieving an ideal breeding state of 'having few diseases and being died'. Therefore, the traditional Chinese medicine composition adopts raw materials including astragalus, codonopsis pilosula, honeysuckle, angelica, dandelion, fructus forsythiae, liquorice, folium isatidis and rehmannia, can enhance the immunity of the organism, improve the disease resistance and achieve the purpose of preventing the African swine fever.
In the embodiment of the invention, the astragalus is 10 to 12 parts by weight; in a preferred embodiment, the astragalus is 10 parts by weight.
In the embodiment of the invention, the weight portion of the radix codonopsitis is 10-12 portions; in a preferred embodiment, the parts by weight of turmeric root-tuber are 12 parts.
In the embodiment of the invention, the honeysuckle is 10-12 parts by weight; in a preferred embodiment, the honeysuckle is 11 parts by weight.
In the embodiment of the invention, the weight portion of the angelica is 8-10; in a preferred embodiment, the weight portion of the angelica is 9 portions.
In the embodiment of the invention, the dandelion is 8-10 parts by weight; in a preferred embodiment, the dandelion is 8 parts by weight.
In the embodiment of the invention, the weight part of the forsythia is 8-10 parts; in a preferred embodiment, the parts by weight of forsythia suspense are 10 parts.
In the embodiment of the invention, the liquorice is 4 to 6 parts by weight; in a preferred embodiment, the licorice is 5 parts by weight.
In the embodiment of the invention, the dyers woad leaf is 6 to 8 parts by weight; in a preferred embodiment, the folium isatidis is 7 parts by weight.
In the embodiment of the invention, the weight portion of the rehmannia is 8-10 portions; in a preferred embodiment, the rehmannia root is 9 parts by weight.
The invention also provides a preparation method of the Chinese herbal medicine preparation for preventing African swine fever virus, which comprises the following steps:
1) cleaning radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, Glycyrrhrizae radix, folium Isatidis, and rehmanniae radix, and oven drying;
2) weighing the raw materials in the step 1) in proportion, soaking the raw materials in ethanol for 30min, then carrying out ultrasonic treatment for 15min, carrying out reflux extraction for 2h for the first time, and filtering the extract by using sixteen layers of gauze to obtain a filtrate;
3) adding ethanol into the dregs obtained in the step 2), performing reflux extraction for the second time for 1 hour, filtering with sixteen layers of gauze to obtain filtrate, and combining the two filtrates to obtain a liquid medicine;
4) and (3) rotatably evaporating the liquid medicine obtained in the step 3) until no alcohol smell exists, adding water and white sugar into the concentrated solution, heating and boiling for 5min, cooling to room temperature, adding streptococcus faecalis, fermenting for 72h, and vacuum drying to obtain the Chinese herbal medicine preparation for preventing African swine fever viruses.
Wherein, the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia are all the same as the above, and are not repeated herein.
According to the invention, various traditional Chinese medicine raw materials are mixed and fermented, so that the medicinal activity of the traditional Chinese medicine is improved, and the drug resistance of pathogenic bacteria is reduced on the premise of ensuring good curative effect; the product also has the characteristics of improving and regulating the body state and the function of improving the body immunity, has special fermentation fragrance, has good palatability and can improve the feed intake of animals.
In the embodiment of the invention, the mass concentration of the ethanol adopted in the first reflux extraction is 80%, and the adding amount of the ethanol is 10 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the folium isatidis and the rehmannia.
In the embodiment of the invention, the mass concentration of the ethanol adopted in the second reflux extraction is 60%, and the adding amount of the ethanol is 8 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the folium isatidis and the rehmannia.
The mass concentration of ethanol is 80%, and the mass concentration of ethanol is 60%, which refers to the mass concentration of ethanol and water, wherein the mass concentration of ethanol is 80%, and the mass concentration of ethanol of 80% refers to 80 parts by weight of ethanol and 20 parts by weight of water.
In the embodiment of the invention, the ratio of the volume of water to the mass of the concentrated solution is 1: 1, the adding amount of the white sugar is 1.5 percent of the mass of the concentrated solution.
Wherein the inoculation amount of the streptococcus faecalis is 3 multiplied by 106-3×107cfu/g, that is, the inoculation amount of streptococcus faecalis in 1g of mixed liquid medicine after being cooled to normal temperature is 3 multiplied by 106-3×107cfu。
In the examples of the present invention, the temperature of vacuum drying was 37 ℃.
To further illustrate the present invention, the following examples are provided to describe a herbal formulation for preventing african swine fever virus and a method for preparing the same in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
The Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following raw materials in percentage by weight:
10 parts of astragalus membranaceus, 10 parts of codonopsis pilosula, 10 parts of honeysuckle, 8 parts of angelica sinensis, 8 parts of dandelion, 8 parts of fructus forsythiae, 4 parts of liquorice, 6 parts of folium isatidis and 8 parts of rehmannia glutinosa.
The preparation method of the Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following steps:
1) cleaning radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, Glycyrrhrizae radix, folium Isatidis, and rehmanniae radix, and oven drying;
2) weighing the raw materials in the step 1) in proportion, soaking the raw materials in ethanol for 30min, then carrying out ultrasonic treatment for 15min, carrying out reflux extraction for 2h for the first time, and filtering the extract by using sixteen layers of gauze to obtain a filtrate; the mass concentration of the ethanol is 80 percent, and the adding amount of the ethanol is 10 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
3) adding ethanol into the dregs obtained in the step 2), performing reflux extraction for the second time for 1 hour, filtering with sixteen layers of gauze to obtain filtrate, and combining the two filtrates to obtain a liquid medicine; the mass concentration of the ethanol is 60 percent, and the adding amount of the ethanol is 8 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
4) rotationally evaporating the liquid medicine obtained in the step 3) until no alcohol smell exists, adding water and white sugar into the concentrated solution, heating and boiling for 5min, cooling to room temperature, adding streptococcus faecalis, fermenting for 72h, and then drying in vacuum at the temperature of 37 ℃ to obtain a Chinese herbal medicine preparation for preventing African swine fever viruses;
the mass ratio of the volume of water to the concentrated solution is 1: 1, the adding amount of the white sugar is 1.5 percent of the mass of the concentrated solution, and the inoculation amount of the streptococcus faecalis is 3 multiplied by 106cfu/g。
Example 2
The Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following raw materials in percentage by weight:
12 parts of astragalus membranaceus, 12 parts of codonopsis pilosula, 12 parts of honeysuckle, 10 parts of angelica sinensis, 10 parts of dandelion, 10 parts of fructus forsythiae, 6 parts of liquorice, 8 parts of folium isatidis and 10 parts of rehmannia glutinosa.
The preparation method of the Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following steps:
1) cleaning radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, Glycyrrhrizae radix, folium Isatidis, and rehmanniae radix, and oven drying;
2) weighing the raw materials in the step 1) in proportion, soaking the raw materials in ethanol for 30min, then carrying out ultrasonic treatment for 15min, carrying out reflux extraction for 2h for the first time, and filtering the extract by using sixteen layers of gauze to obtain a filtrate; the mass concentration of the ethanol is 80 percent, and the adding amount of the ethanol is 10 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
3) adding ethanol into the dregs obtained in the step 2), performing reflux extraction for the second time for 1 hour, filtering with sixteen layers of gauze to obtain filtrate, and combining the two filtrates to obtain a liquid medicine; the mass concentration of the ethanol is 60 percent, and the adding amount of the ethanol is 8 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
4) rotationally evaporating the liquid medicine obtained in the step 3) until no alcohol smell exists, adding water and white sugar into the concentrated solution, heating and boiling for 5min, cooling to room temperature, adding streptococcus faecalis, fermenting for 72h, and then drying in vacuum at the temperature of 37 ℃ to obtain a Chinese herbal medicine preparation for preventing African swine fever viruses;
the mass ratio of the volume of water to the concentrated solution is 1: 1, the adding amount of the white sugar is 1.5 percent of the mass of the concentrated solution, and the inoculation amount of the streptococcus faecalis is 3 multiplied by 107cfu/g。
Example 3
The Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following raw materials in percentage by weight:
11 parts of astragalus, 11 parts of codonopsis pilosula, 10 parts of honeysuckle, 8 parts of angelica, 9 parts of dandelion, 9 parts of fructus forsythiae, 4 parts of liquorice, 8 parts of folium isatidis and 10 parts of rehmannia.
The preparation method of the Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following steps:
1) cleaning radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, Glycyrrhrizae radix, folium Isatidis, and rehmanniae radix, and oven drying;
2) weighing the raw materials in the step 1) in proportion, soaking the raw materials in ethanol for 30min, then carrying out ultrasonic treatment for 15min, carrying out reflux extraction for 2h for the first time, and filtering the extract by using sixteen layers of gauze to obtain a filtrate; the mass concentration of the ethanol is 80 percent, and the adding amount of the ethanol is 10 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
3) adding ethanol into the dregs obtained in the step 2), performing reflux extraction for the second time for 1 hour, filtering with sixteen layers of gauze to obtain filtrate, and combining the two filtrates to obtain a liquid medicine; the mass concentration of the ethanol is 60 percent, and the adding amount of the ethanol is 8 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
4) rotationally evaporating the liquid medicine obtained in the step 3) until no alcohol smell exists, adding water and white sugar into the concentrated solution, heating and boiling for 5min, cooling to room temperature, adding streptococcus faecalis, fermenting for 72h, and then drying in vacuum at the temperature of 37 ℃ to obtain a Chinese herbal medicine preparation for preventing African swine fever viruses;
the mass ratio of the volume of water to the concentrated solution is 1: 1, the adding amount of the white sugar is 1.5 percent of the mass of the concentrated solution, and the inoculation amount of the streptococcus faecalis is 1 multiplied by 107cfu/g。
Example 4
The Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following raw materials in percentage by weight:
10 parts of astragalus, 12 parts of codonopsis pilosula, 11 parts of honeysuckle, 9 parts of angelica, 8 parts of dandelion, 10 parts of fructus forsythiae, 5 parts of liquorice, 7 parts of folium isatidis and 9 parts of rehmannia.
The preparation method of the Chinese herbal medicine preparation for preventing the African swine fever virus comprises the following steps:
1) cleaning radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, Glycyrrhrizae radix, folium Isatidis, and rehmanniae radix, and oven drying;
2) weighing the raw materials in the step 1) in proportion, soaking the raw materials in ethanol for 30min, then carrying out ultrasonic treatment for 15min, carrying out reflux extraction for 2h for the first time, and filtering the extract by using sixteen layers of gauze to obtain a filtrate; the mass concentration of the ethanol is 80 percent, and the adding amount of the ethanol is 10 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
3) adding ethanol into the dregs obtained in the step 2), performing reflux extraction for the second time for 1 hour, filtering with sixteen layers of gauze to obtain filtrate, and combining the two filtrates to obtain a liquid medicine; the mass concentration of the ethanol is 60 percent, and the adding amount of the ethanol is 8 times of the total mass of the astragalus, the codonopsis pilosula, the honeysuckle, the angelica, the dandelion, the forsythia, the liquorice, the dyers woad leaf and the rehmannia;
4) rotationally evaporating the liquid medicine obtained in the step 3) until no alcohol smell exists, adding water and white sugar into the concentrated solution, heating and boiling for 5min, cooling to room temperature, adding streptococcus faecalis, fermenting for 72h, and then drying in vacuum at the temperature of 37 ℃ to obtain a Chinese herbal medicine preparation for preventing African swine fever viruses;
the mass ratio of the volume of water to the concentrated solution is 1: 1, the adding amount of the white sugar is 1.5 percent of the mass of the concentrated solution, and the inoculation amount of the streptococcus faecalis is 8 multiplied by 106cfu/g。
In the test, 80 piglets of 15-20kg are selected as test animals and are raised for a long time; 80 test animals were randomly divided into two groups, one group being a control group and two groups being test groups, each group being two replicates. The test group adds the Chinese herbal medicine preparation for preventing African swine fever virus prepared in the embodiment 4 into the feed, and the addition amount is 1t of feed and 1.5Kg of the Chinese herbal medicine preparation for preventing African swine fever virus prepared in the embodiment 4; the control group was fed with feed only. Wherein the feed in the test group and the control group is Hefeng pig feed, the two groups of animals are subjected to feeding management under the same condition, and the two groups of piglets are continuously fed for 40 days. After the test is finished, all the test pigs are deeply buried for harmless treatment.
The clinical observation indexes are as follows: the mental state, growth rate, morbidity and mortality of piglets are mainly observed, and the results are shown in table 1.
In the test process, because safety protection is not carried out, the control group and the test group are infected with the African swine fever virus, and the control group, the test group and the pig blood are detected to have the African swine fever virus. The mental state and the nutritional state of the piglets in the control group are poorer than those of the animals in the test group, and a large amount of death occurs. The Chinese herbal medicine preparation for preventing African swine fever virus prepared in example 4 is added into the feed for the test group, the death rate is not high, and the specific data are shown in Table 1.
Table 1 statistical table of test effects
In addition, tests show that the Chinese herbal medicine additive has obvious curative effects on enhancing the immunity of organisms and improving the disease resistance, and adverse reactions such as liver and kidney function damage and the like do not occur when the Chinese herbal medicine additive is taken, so that the Chinese herbal medicine additive has better safety.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A Chinese herbal medicine preparation for preventing African swine fever virus is characterized by comprising the following raw materials in percentage by weight:
10-12 parts of astragalus membranaceus, 10-12 parts of codonopsis pilosula, 10-12 parts of honeysuckle, 8-10 parts of angelica sinensis, 8-10 parts of dandelion, 8-10 parts of fructus forsythiae, 4-6 parts of liquorice, 6-8 parts of folium isatidis and 8-10 parts of rehmannia glutinosa.
2. The herbal formulation for preventing african swine fever virus according to claim 1, comprising the following raw materials in weight percent:
10 parts of astragalus, 12 parts of codonopsis pilosula, 11 parts of honeysuckle, 9 parts of angelica, 8 parts of dandelion, 10 parts of fructus forsythiae, 5 parts of liquorice, 7 parts of folium isatidis and 9 parts of rehmannia.
3. A method of preparing a herbal formulation for the prevention of african swine fever virus according to claim 1 or 2, comprising the steps of:
1) cleaning radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, Glycyrrhrizae radix, folium Isatidis, and rehmanniae radix, and oven drying;
2) weighing the raw materials in the step 1) in proportion, soaking the raw materials in ethanol for 30min, then carrying out ultrasonic treatment for 15min, carrying out reflux extraction for 2h for the first time, and filtering the extract by using sixteen layers of gauze to obtain a filtrate;
3) adding ethanol into the dregs obtained in the step 2), performing reflux extraction for the second time for 1 hour, filtering with sixteen layers of gauze to obtain filtrate, and combining the two filtrates to obtain a liquid medicine;
4) and (3) rotatably evaporating the liquid medicine obtained in the step 3) until no alcohol smell exists, adding water and white sugar into the concentrated solution, heating and boiling for 5min, cooling to room temperature, adding streptococcus faecalis, fermenting for 72h, and vacuum drying to obtain the Chinese herbal medicine preparation for preventing African swine fever viruses.
4. The method for preparing a herbal preparation for preventing African swine fever virus according to claim 3, wherein in the step 2), the mass concentration of ethanol is 80%, and the addition amount of ethanol is 10 times of the total mass of radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, radix Glycyrrhizae, folium Isatidis, and radix rehmanniae.
5. The method for preparing a herbal preparation for preventing African swine fever virus according to claim 3, wherein the ethanol added in step 3) is 60% by mass, and the amount of ethanol added is 8 times of the total mass of radix astragali, radix Codonopsis, flos Lonicerae, radix Angelicae sinensis, herba Taraxaci, fructus forsythiae, radix Glycyrrhizae, folium Isatidis, and radix rehmanniae.
6. The method for preparing the herbal preparation for preventing African swine fever virus according to claim 3, wherein in the step 4), the ratio of the volume of water to the mass of the concentrated solution is 1: 1, the adding amount of the white sugar is 1.5 percent of the mass of the concentrated solution.
7. The method for preparing the herbal preparation for preventing African swine fever virus according to claim 3, wherein the amount of Streptococcus faecalis inoculated in step 4) is 3 x 106-3×107cfu/g。
8. The method for preparing a herbal formulation for the prevention of African swine fever virus according to claim 3, wherein the temperature of vacuum drying is 37 ℃.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113198009A (en) * | 2021-06-23 | 2021-08-03 | 长江大学 | Oral liquid for treating African swine fever and preparation method thereof |
| CN113508877A (en) * | 2021-07-20 | 2021-10-19 | 齐齐哈尔医学院 | Preparation method of compound fermented feed for dairy cows |
| CN114470148A (en) * | 2021-11-26 | 2022-05-13 | 国众兴合生物医药科技有限公司 | Natural compound biological agent for resisting swine fever virus as well as preparation method and application thereof |
| CN114886975A (en) * | 2022-05-27 | 2022-08-12 | 焦作市保程伟业生物科技有限公司 | Traditional Chinese veterinary medicine for preventing and treating swine fever and preparation method thereof |
| CN115487250A (en) * | 2022-09-20 | 2022-12-20 | 广东海大集团股份有限公司 | Traditional Chinese medicine composition for preventing and treating African swine fever and preparation method and application thereof |
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2019
- 2019-12-31 CN CN201911410215.XA patent/CN111067974A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113198009A (en) * | 2021-06-23 | 2021-08-03 | 长江大学 | Oral liquid for treating African swine fever and preparation method thereof |
| CN113508877A (en) * | 2021-07-20 | 2021-10-19 | 齐齐哈尔医学院 | Preparation method of compound fermented feed for dairy cows |
| CN114470148A (en) * | 2021-11-26 | 2022-05-13 | 国众兴合生物医药科技有限公司 | Natural compound biological agent for resisting swine fever virus as well as preparation method and application thereof |
| CN114886975A (en) * | 2022-05-27 | 2022-08-12 | 焦作市保程伟业生物科技有限公司 | Traditional Chinese veterinary medicine for preventing and treating swine fever and preparation method thereof |
| CN115487250A (en) * | 2022-09-20 | 2022-12-20 | 广东海大集团股份有限公司 | Traditional Chinese medicine composition for preventing and treating African swine fever and preparation method and application thereof |
| CN115487250B (en) * | 2022-09-20 | 2023-12-19 | 广东海大集团股份有限公司 | Traditional Chinese medicine composition for preventing and treating African swine fever as well as preparation method and application thereof |
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Application publication date: 20200428 |