CN104161181A - Feed additive for preventing pig bacterial diseases, preparation method and application thereof - Google Patents
Feed additive for preventing pig bacterial diseases, preparation method and application thereof Download PDFInfo
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Abstract
The present invention discloses a feed additive for preventing pig bacterial diseases, a preparation method and an application thereof. The feed additive comprises the following ingredients by weight: 2-5 parts of egg yolk antibody lyophilized powder for preventing pig bacterial diseases, 5-10 parts of astragalus polysaccharide, 10-15 parts of barbary wolfberry polysaccharide, 10-20 parts of glycyrrhiza polysaccharide, 10-15 parts of heartleaf houttuynia herb, 5-10 parts of Chinese goldthread rhizome and 9-21 parts of male inflorescence of populus. According to the present invention, the feed additive for preventing pig bacterial diseases is prepared by compounding the egg yolk antibody lyophilized powder for preventing pig bacterial diseases, the astragalus polysaccharide, the wolfberry polysaccharide, the glycyrrhiza polysaccharide, the heartleaf houttuynia herb, the Chinese goldthread rhizome and the male inflorescence of populus, and is added into the daily feed so as to improve the immunity and the disease resistance of animals. Experimental results show that after the feed additive in the present invention is added to the feed, the effect of preventing pig bacterial diseases is significantly improved, and the prevention effect is obviously superior to the prevention effect resulting from the feed to which the egg yolk antibody lyophilized powder or the traditional Chinese medicine composition is singly added.
Description
Technical Field
The invention relates to a feed additive for resisting pig bacterial diseases, and also relates to a preparation method and application of the feed additive, belonging to the field of feeds.
Background
With the development of society and the improvement of living standard of people, more and more people aim at food safety. Pork is a meat product mainly eaten by residents in China, and the breeding quantity in China is large. China is a country with large pig production and consumption, the scale of the pig raising industry is gradually enlarged, but the spreading of pig diseases is common due to the limitation of feeding conditions. The pig has more bacterial diseases, the economic loss caused by each year reaches more than hundreds of millions, and the production performance reduction loss caused by each year also reaches more than hundreds of millions, so that the prevention of the pig bacterial diseases is inevitable.
At present, the common methods for preventing and treating the bacterial diseases of the pigs are more, and western medicines, vaccination and Chinese herbal medicines are mainly used for preventing and treating the bacterial diseases of the pigs. The inundation of western medicines brings great dietary hidden troubles for the eating of pig products, and the medicine residue becomes one of the main factors influencing the safety of the pig products. The early effect of the inoculation immunity is good, but multiple times of inoculation are needed, the operation is troublesome, the cost is high, and the stress reaction of the young pigs is easy to cause; the vaccine has high requirements on preservation conditions, needs to be refrigerated at the temperature of 2-8 ℃, and loses effect for a period of time at room temperature. The Chinese herbal medicine feed additive is also one of the current research directions, but because the variety of the Chinese herbal medicine is more, the effect difference of the same Chinese herbal medicine on different livestock diseases is great, and the immune effect of the Chinese herbal medicine on the diseases is extremely limited, the application of the Chinese herbal medicine feed additive also has certain limitation. Injection of yolk antibody is also a common method, but the immune effect of single injection of yolk antibody sometimes cannot meet the actual requirement.
Disclosure of Invention
The invention aims to provide a feed additive for resisting bacterial diseases of pigs, which can effectively prevent bacterial diseases of multiple pigs.
In order to achieve the aim, the technical scheme adopted by the invention is to provide a feed additive for resisting the bacterial diseases of pigs, which comprises the following components in parts by weight: 2-5 parts of pig bacterial disease resistant yolk antibody freeze-dried powder, 5-10 parts of astragalus polysaccharide, 10-15 parts of lycium barbarum polysaccharide, 10-20 parts of glycyrrhiza polysaccharide, 10-15 parts of houttuynia cordata, 5-10 parts of coptis chinensis and 9-21 parts of poplar flower.
The feed additive for resisting the pig bacterial diseases comprises the following components in parts by weight: 2-5 parts of pig bacterial disease resistant yolk antibody freeze-dried powder, 5-10 parts of astragalus polysaccharide, 10-15 parts of lycium barbarum polysaccharide, 10-20 parts of glycyrrhiza polysaccharide, 10-15 parts of houttuynia cordata, 5-10 parts of coptis chinensis and 10-20 parts of poplar flower.
The swine bacterial disease resistant yolk antibody freeze-dried powder comprises the following components in parts by weight: 93-96 parts of a swine bacterial disease resistant yolk antibody, 0.01-0.02 part of a preservative, 0.2-0.3 part of an inactivator, 0.5-1 part of vitamin C, 1-2 parts of polyvinylpyrrolidone, 0.5-1 part of glycine, 1-2 parts of sorbitol and 2-4 parts of glucose.
The inactivator is formaldehyde.
The preservative is thimerosal.
The invention also aims to provide a preparation method of the feed additive for resisting the pig bacterial diseases.
The technical scheme adopted by the invention is to provide a preparation method of a feed additive for resisting pig bacterial diseases, which comprises the following steps:
1) proliferating and inactivating pathogenic bacteria of pig bacterial diseases to prepare a vaccine, immunizing healthy chicken flocks to prepare hyperimmune eggs, and sterilizing, acidifying, centrifuging, preparing and freeze-drying the hyperimmune eggs to obtain the freeze-dried powder of the egg yolk antibody for resisting the pig bacterial diseases;
2) mixing the lyophilized powder with the rest components.
The preparation method of the feed additive for resisting the pig bacterial diseases specifically comprises the following steps:
1) proliferating and inactivating pathogenic bacteria of the bacterial diseases of the pigs to prepare a vaccine, immunizing healthy laying hen groups for three times every 7 days, starting to detect the antibody titer after the second immunization, and when the antibody titer reaches 1: 256 times, collecting high immunity eggs, cleaning, sterilizing, and air drying;
2) separating highly-immune egg yolk, adding sterile water with the same volume, and fully stirring to obtain a yolk antibody solution; uniformly heating the egg yolk antibody solution in a water bath kettle at 60-65 ℃ for 30min, adding an acidified aqueous solution with the pH =4.9-5.2 and the temperature of 4-6 ℃, uniformly mixing, standing for precipitation, taking a supernatant, and centrifuging at 15000rpm for 20min to obtain a supernatant of the egg yolk antibody;
3) filtering the supernatant obtained in the step 2) by using a 0.45-micrometer filter membrane, intercepting macromolecular substances with the molecular weight of more than 200KD, and filtering the filtrate by using a 0.22-micrometer filter membrane to obtain filtrate;
4) concentrating the filtrate by using an ultrafiltration device to concentrate the egg yolk antibody solution to 2.5mL/500 feather;
5) adding an inactivating agent, a preservative, vitamin C, polyvinylpyrrolidone, glycine, sorbitol and glucose into the egg yolk antibody solution obtained in the step 4), and fully freezing at the temperature of minus 80 ℃;
6) transferring the precooled yolk antibody solution in the step 5) into a freeze dryer to prepare the pig bacterial disease resistant yolk antibody freeze-dried powder;
7) mixing the lyophilized powder with radix astragali polysaccharide, fructus Lycii polysaccharide, Glycyrrhrizae radix polysaccharide, herba Houttuyniae, Coptidis rhizoma, and poplar flower.
The invention also aims to provide the application of the feed additive in preparing the feed for resisting the pig bacterial diseases.
The technical scheme adopted by the invention also provides the application of the feed additive in preparing the feed for resisting the pig bacterial diseases, and the addition amount of the feed additive is 3-7% of the total weight of the feed.
The swine bacterial disease is common swine bacterial disease, such as swine erysipelas, swine plague, swine colibacillosis and swine red dysentery in the embodiment of the invention.
The yolk antibody is an immunoglobulin, and can be combined with corresponding antigen to eliminate etiology due to a stress product generated by the stimulation of the body by the external antigen.
The invention adopts healthy chicken as the immune object, reduces the possibility of virus strain reversion, and can also make the antibody titer quickly reach 1: 256. the invention compounds the yolk antibody resisting the porcine bacterial disease and various auxiliary materials, greatly prolongs the preservation time of the yolk antibody and ensures the activity of the yolk antibody in a longer period of time by adjusting the components and controlling the dosage of the auxiliary materials.
The polysaccharide has effects of improving immunity, enhancing adaptability and resistance of organism, resisting bacteria, regulating metabolism, etc. Wherein,
astragalus polysaccharides is the main effective component of Astragalus, and Astragalus is sweet and warm. It enters lung and spleen meridians. Tonify qi, raise yang, strengthen superficial resistance, arrest sweating, induce diuresis to alleviate edema, remove toxicity and promote tissue regeneration. It is indicated for all syndromes of qi deficiency and blood deficiency. Mainly acts on the kidney for promoting diuresis, promotes the DNA synthesis in cells, accelerates the differentiation and proliferation of the liver, improves the proliferation of lymphocytes and the enhancement of the capacity of leukocyte induced interferon, and indirectly improves and promotes the immunity function of organisms.
The lycium barbarum polysaccharide is the main effective component of lycium barbarum, and is lycium barbarum, sweet and neutral. It enters liver, kidney and lung meridians. Nourish liver, moisten and moisten lung. It can be used for treating deficiency of liver and kidney, dizziness, blurred vision, soreness of waist and knees, sexual impotence, spermatorrhea, cough due to asthenia, diabetes, and polydipsia. Mainly acts on organs such as liver and kidney, can regulate the immunologic function of organisms, can effectively inhibit the growth of tumors and cell mutation, and has the effects of delaying senility, resisting fatty liver, regulating blood fat and blood sugar, promoting the hematopoietic function and the like.
The glycyrrhiza polysaccharide is the main effective component of glycyrrhiza, and glycyrrhiza is sweet and neutral. The medicine has the functions of invigorating spleen, benefiting vital energy, clearing away heat and toxic matter, eliminating phlegm, stopping cough, relieving pain and harmonizing the recipe. Can be used for treating weakness of spleen and stomach, asthenia, palpitation, short breath, cough, excessive phlegm, and relieving drug toxicity.
Yu xing Cao is pungent and slightly cold in nature. It enters lung, bladder and large intestine meridians. Clearing away heat and toxic material, expelling pus, resolving carbuncle, inducing diuresis, and treating stranguria, and can be used for treating pulmonary abscess, suppuration, cough and asthma due to lung heat, tonsillitis, swelling, sore, hemorrhoid, dysentery, pyretic stranguria, edema, leukorrhagia, scabies and tinea. Has antibacterial, antiviral, immunity enhancing, and diuretic effects.
Huang Lian is bitter and cold in nature. It enters heart, liver, stomach and large intestine meridians. Clear heat and purge fire, dry dampness, remove toxicity. The traditional Chinese medicine composition is mainly used for treating high fever and dysphoria of heart channels of people with fever, hematemesis and epistaxis caused by delirium or excessive heat forcing blood to flow recklessly, damp-heat chest stuffiness, diarrhea, dysentery, vexation and insomnia caused by excessive heart fire, stomach heat vomiting, trough and hunger eliminating, liver fire and conjunctival congestion and swelling and pain, heat-toxin sores and ulcers, furunculosis and jaundice, gingival swelling and pain, mouth and tongue sores, purulent otitis media, hemorrhoidal bleeding, eczema and scald. Has antimicrobial, antiprotozoal, antiviral, and antitumor effects.
Poplar flower, radix Poplar flower, has the effects of clearing away heat and toxic material, eliminating dampness and relieving dysentery. It can be used for treating bacillary dysentery and enteritis.
The Chinese herbal medicines have the functions of regulating the immunity of the organism, enhancing the anti-stress capability of the organism, protecting the viscera and enhancing the direct action of the yolk antibody on the organism.
The pig bacterial disease resistant feed additive is prepared by compounding the pig bacterial disease resistant yolk antibody freeze-dried powder and traditional Chinese medicine compositions of astragalus polysaccharide, lycium barbarum polysaccharide, glycyrrhiza polysaccharide, houttuynia cordata, coptis chinensis, poplar flowers and the like, has the advantages of strong pertinence, high antibody titer and good biological activity, can play a role in effectively preventing pig bacterial diseases, and can still maintain the activity after being stored at normal temperature for a long time.
The feed additive disclosed by the invention has no toxic or side effect, no residual hazard, safety and effectiveness, and has a good prevention effect on pig bacterial diseases, especially bacterial diseases such as pig erysipelas, pig lung plague, pig transmissible gastroenteritis, pig red dysentery and the like. The feed additive adopts corresponding egg yolk antibodies according to different bacterial diseases of pigs, and has good prevention effect by matching with Chinese herbal medicines.
The invention reasonably matches and combines the yolk antibody freeze-dried powder for resisting the porcine bacterial diseases and the traditional Chinese medicine composition, and the yolk antibody freeze-dried powder can improve the immunity and disease resistance of organisms when being added into daily feed. Experimental results show that after the feed additive is fed to weaned pigs, the bacterial disease resistance of the weaned pigs is obviously improved, and the bacterial disease resistance effect of the weaned pigs is obviously superior to that of the pigs which are separately added with the yolk antibody freeze-dried powder or the traditional Chinese medicine composition.
Detailed Description
Example 1
The swine erysipelas resisting yolk antibody freeze-dried powder comprises the following components in parts by weight: 93 parts of swine erysipelas-resisting yolk antibody, 0.01 part of thimerosal, 0.3 part of formaldehyde, 0.5 part of vitamin C, 1 part of polyvinylpyrrolidone, 1 part of glycine, 1 part of sorbitol and 2 parts of glucose.
The feed additive for resisting swine erysipelas comprises the following components in parts by weight: 4 parts of swine erysipelas-resistant yolk antibody freeze-dried powder, 5 parts of astragalus polysaccharide, 10 parts of lycium barbarum polysaccharide, 20 parts of glycyrrhiza polysaccharide, 10 parts of houttuynia cordata, 10 parts of coptis chinensis and 15 parts of poplar flower.
The preparation method of the feed additive for resisting swine erysipelas comprises the following steps:
1) separating strains to prepare vaccines: multiplying and inactivating a standard swine erysipelas strain G4T10 to prepare an emulsifiable agent vaccine for later use;
2) obtaining eggs by immune healthy chicken groups: immunizing healthy chickens once every 7 days and three times in total, inoculating 1.5mL of inactivated vaccine to the healthy chickens immunized for the first time, adding 1 time of the inactivated vaccine to the healthy chickens immunized for the second time and the healthy chickens immunized for the third time, starting to detect the antibody titer after the second immunization, and when the antibody titer reaches 1: 256 times, collecting high immunity eggs, cleaning, sterilizing, and air drying;
3) acidifying: separating highly-immune egg yolk, adding sterile water with the same volume, and fully stirring to obtain a yolk antibody solution; uniformly heating the egg yolk antibody solution in a water bath kettle at 63 ℃ for 30min, adding an acidified aqueous solution with pH =5 and temperature of 5 ℃, uniformly mixing, standing for precipitation, taking supernatant, centrifuging at 15000rpm for 20min, and obtaining supernatant containing the egg yolk antibody;
4) filtering the supernatant obtained in the step 3) by using a 0.45-micrometer filter membrane, intercepting macromolecular substances with the molecular weight of more than 200KD, and filtering the filtrate by using a 0.22-micrometer filter membrane to obtain filtrate;
5) the filtrate was concentrated by an ultrafiltration apparatus to concentrate the egg yolk antibody solution to 2.5mL/500 feather.
6) Adding formaldehyde, thimerosal, vitamin C, polyvinylpyrrolidone, glycine, sorbitol and glucose into the concentrated egg yolk antibody solution;
7) placing the yolk solution obtained in the step 6) at-80 ℃, and fully freezing;
8) transferring the precooled yolk antibody solution obtained in the step 7) into a freeze dryer to prepare swine erysipelas resistant yolk antibody freeze-dried powder;
9) mixing the swine erysipelas resisting yolk antibody lyophilized powder with astragalus polysaccharide, lycium polysaccharide, glycyrrhiza polysaccharide, houttuynia cordata, coptis chinensis and poplar flower.
Example 2
The anti-swine plague yolk antibody freeze-dried powder comprises the following components in parts by weight: 95 parts of swine plague resistant yolk antibody, 0.02 part of merthiolate, 0.2 part of formaldehyde, 1 parts of vitamin C, 1.5 parts of polyvinylpyrrolidone, 0.5 part of glycine, 1.5 parts of sorbitol and 3 parts of glucose.
The feed additive for resisting swine plague comprises the following components in parts by weight: 5 parts of swine plague resistant yolk antibody freeze-dried powder, 8 parts of astragalus polysaccharide, 15 parts of lycium barbarum polysaccharide, 10 parts of glycyrrhiza polysaccharide, 15 parts of houttuynia cordata, 5 parts of coptis chinensis and 10 parts of poplar flower.
The preparation method of the feed additive for resisting swine plague is the same as that in example 1, except that the pathogenic bacteria of the swine bacterial disease in the example are the swine plague standard strain EO630 strain, and the dosage of each component is different.
Example 3
The swine colibacillosis resistant yolk antibody freeze-dried powder comprises the following components in parts by weight: 96 parts of pig colibacillosis resistant yolk antibody, 0.015 part of thimerosal, 0.25 part of formaldehyde, 0.8 part of vitamin C, 2 parts of polyvinylpyrrolidone, 0.8 part of glycine, 2 parts of sorbitol and 4 parts of glucose.
The feed additive for resisting the swine colibacillosis comprises the following components in parts by weight: 2 parts of pig colibacillosis resistant yolk antibody freeze-dried powder, 10 parts of astragalus polysaccharide, 13 parts of lycium barbarum polysaccharide, 15 parts of glycyrrhiza polysaccharide, 12 parts of houttuynia cordata, 8 parts of coptis chinensis and 20 parts of poplar flower.
The preparation method of the feed additive for resisting the swine colibacillosis in the embodiment is the same as that in the embodiment 1, except that the pathogenic bacteria of the swine bacterial disease in the embodiment are swine colibacillosis standard strain K88 strains, and the dosage of each component is different.
Example 4
The swine dysentery resistant yolk antibody freeze-dried powder comprises the following components in parts by weight: 94 parts of swine red dysentery resistant yolk antibody, 0.02 part of merthiolate, 0.2 part of formaldehyde, 1 parts of vitamin C, 1.7 parts of polyvinylpyrrolidone, 0.9 part of glycine, 1.5 parts of sorbitol and 2 parts of glucose.
The feed additive for resisting swine dysentery of the embodiment comprises the following components in parts by weight: 5 parts of swine dysentery resistant yolk antibody freeze-dried powder, 8 parts of astragalus polysaccharide, 15 parts of lycium barbarum polysaccharide, 10 parts of glycyrrhiza polysaccharide, 15 parts of houttuynia cordata, 5 parts of coptis chinensis and 10 parts of poplar flower.
The preparation method of the feed additive for resisting swine red dysentery is the same as that in example 1, except that the pathogenic bacteria of the swine bacterial disease in the example are swine red dysentery standard strain type C perinatal clostridium perfringens, and the use amounts of the components are different.
Experimental example 1
Quality detection of the feed additive of the invention
The experimental method comprises the following steps: the feed additive of the present invention was stored in a 37 ℃ incubator for 24 months, and after 1 week, 2 weeks, 1 month, 2 months, 4 months, 8 months, 12 months, 16 months, 20 months and 24 months, respectively, the change in the yolk antibody titer of samples at each time point was measured by the agar diffusion (AGP) method, and the results are shown in table 1.
TABLE 1 Change in potency of lyophilized powder at Normal temperature for different periods of time
| Examples | Item | 1 week | 2 weeks | 1 month | 2 month | 4 month | 8 month | 12 month | 16 months old | 20 months old | 24 months |
| 1 | Erysipelas of pig | 1：32 | 1：32 | 1：32 | 1：32 | 1：32 | 1：32 | 1：16 | 1：16 | 1：16 | 1：16 |
| 2 | Swine plague | 1：32 | 1：32 | 1：32 | 1：32 | 1：32 | 1：16 | 1：16 | 1：16 | 1：16 | 1：16 |
| 3 | Escherichia coli of pig | 1：32 | 1：32 | 1：32 | 1：32 | 1：32 | 1：32 | 1：16 | 1：16 | 1：16 | 1：16 |
| 4 | Dysentery of pig | 1：32 | 1：32 | 1：32 | 1：32 | 1：32 | 1：16 | 1：16 | 1：16 | 1：16 | 1：16 |
Detecting the quality of the yolk antibody:
the mycoplasma test was performed according to the Chinese veterinary pharmacopoeia, and no mycoplasma growth occurred.
The test of exogenous bacteria is carried out according to Chinese animal pharmacopoeia, which conforms to the regulations.
[ safety test ] 5 piglets of 7 days old were treated by intramuscular injection of 5 feathers for 2 weeks, and all were kept alive without lesions in the injection site.
The shelf life can be stored for 2 years according to the test result of the titer detection at normal temperature.
Experimental example 2
The effect analysis of the feed additive for resisting swine erysipelas
The ordinary feed in this example was a conventional feed commercially available.
The experimental method comprises the following steps: 500 weaned piglets are selected, wherein 100 weaned piglets are separately fed as a challenge group, 100 weaned piglets are separately fed as a blank group, 200 weaned piglets are separately fed as a control group, and 100 weaned piglets are separately fed as a test group. The toxin counteracting group and the blank group are fed with common feed, the control group is divided into 2 groups, 100 control groups are fed with 7% of anti-swine erysipelas yolk antibody freeze-dried powder, the common feed and 7% of traditional Chinese medicine composition and the common feed, the test group is fed in an isolated mode, and the feed additive of example 1 and the common feed are fed with 7% of additive amount. Feeding freely, drinking freely, after 24h, taking weaned piglets of the test group, the control group and the challenge group, injecting intramuscular, inoculating and diluting erysipelas bacterial liquid, and recording 120h disease conditions, wherein the results are shown in table 2.
TABLE 2 Effect analysis of the feed additive of the present invention against swine erysipelas
Experimental example 3
The effect analysis of the feed additive for resisting swine plague
The experimental method is the same as that of experimental example 2, except that the experimental group of the experimental example is added with the feed additive of the experimental example 2, and the results of the experimental group of the experimental example are shown in table 3.
TABLE 3 Effect analysis of the feed additive of the present invention against swine plague
Experimental example 4
The effect analysis of the feed additive for resisting the swine colibacillosis
The experimental method is the same as that of experimental example 2, except that the experimental group of the experimental example is added with the feed additive of example 3, and the weaned piglets are injected with the diluted bacterial liquid of the porcine colibacillosis, and the results are shown in table 4.
TABLE 4 analysis of the effect of the feed additive of the present invention against colibacillosis in swine
Experimental example 5
Analysis of Effect of the feed additive of the invention on resisting swine dysentery
The experimental method is the same as that of experimental example 2, except that the feed additive of example 4 is added to the experimental group of this experimental example, and the weaned pig is injected with intramuscular inoculation diluted swine dysentery bacterium liquid, and the results are shown in table 5.
TABLE 5 analysis of the anti-swine dysentery effect of the feed additive of the present invention
As can be seen from tables 2-5, after the feed additive of the invention is added, the capability of resisting the pig bacterial diseases is obviously improved, and the effect of resisting the pig bacterial diseases is also obviously superior to the effect of independently adding the yolk antibody freeze-dried powder or the traditional Chinese medicine composition.
Claims (9)
1. The feed additive for resisting the bacterial diseases of pigs is characterized by comprising the following components in parts by weight: 2-5 parts of pig bacterial disease resistant yolk antibody freeze-dried powder, 5-10 parts of astragalus polysaccharide, 10-15 parts of lycium barbarum polysaccharide, 10-20 parts of glycyrrhiza polysaccharide, 10-15 parts of houttuynia cordata, 5-10 parts of coptis chinensis and 9-21 parts of poplar flower.
2. The feed additive for resisting the bacterial diseases of pigs according to claim 1, which is characterized by comprising the following components in parts by weight: 2-5 parts of pig bacterial disease resistant yolk antibody freeze-dried powder, 5-10 parts of astragalus polysaccharide, 10-15 parts of lycium barbarum polysaccharide, 10-20 parts of glycyrrhiza polysaccharide, 10-15 parts of houttuynia cordata, 5-10 parts of coptis chinensis and 10-20 parts of poplar flower.
3. The feed additive for resisting the porcine bacterial diseases according to claim 1, wherein the lyophilized powder of the yolk antibody for resisting the porcine bacterial diseases comprises the following components in parts by weight: 93-96 parts of a swine bacterial disease resistant yolk antibody, 0.01-0.02 part of a preservative, 0.2-0.3 part of an inactivator, 0.5-1 part of vitamin C, 1-2 parts of polyvinylpyrrolidone, 0.5-1 part of glycine, 1-2 parts of sorbitol and 2-4 parts of glucose.
4. The feed additive for resisting swine bacterial diseases according to claim 3, wherein the inactivating agent is formaldehyde.
5. The feed additive for resisting swine bacterial diseases according to claim 3, wherein the preservative is thimerosal.
6. A method for preparing the feed additive for resisting bacterial diseases of pigs according to claim 1, which comprises the following steps:
1) proliferating and inactivating pathogenic bacteria of pig bacterial diseases to prepare a vaccine, immunizing healthy chicken flocks to prepare hyperimmune eggs, and sterilizing, acidifying, centrifuging, preparing and freeze-drying the hyperimmune eggs to obtain the freeze-dried powder of the egg yolk antibody for resisting the pig bacterial diseases;
2) mixing the lyophilized powder with the rest components.
7. The preparation method of the feed additive for resisting the bacterial diseases of pigs according to claim 6, which is characterized by comprising the following steps:
1) proliferating and inactivating pathogenic bacteria of the bacterial diseases of the pigs to prepare a vaccine, immunizing healthy laying hen groups for three times every 7 days, starting to detect the antibody titer after the second immunization, and when the antibody titer reaches 1: 256 times, collecting high immunity eggs, cleaning, sterilizing, and air drying;
2) separating highly-immune egg yolk, adding sterile water with the same volume, and fully stirring to obtain a yolk antibody solution; uniformly heating the egg yolk antibody solution in a water bath kettle at 60-65 ℃ for 30min, adding an acidified aqueous solution with the pH =4.9-5.2 and the temperature of 4-6 ℃, uniformly mixing, standing for precipitation, taking a supernatant, and centrifuging at 15000rpm for 20min to obtain a supernatant of the egg yolk antibody;
3) filtering the supernatant obtained in the step 2) by using a 0.45-micrometer filter membrane, intercepting macromolecular substances with the molecular weight of more than 200KD, and filtering the filtrate by using a 0.22-micrometer filter membrane to obtain filtrate;
4) concentrating the filtrate by using an ultrafiltration device to concentrate the egg yolk antibody solution to 2.5mL/500 feather;
5) adding an inactivating agent, a preservative, vitamin C, polyvinylpyrrolidone, glycine, sorbitol and glucose into the egg yolk antibody solution obtained in the step 4), and fully freezing at the temperature of minus 80 ℃;
6) transferring the precooled yolk antibody solution in the step 5) into a freeze dryer to prepare the pig bacterial disease resistant yolk antibody freeze-dried powder;
7) mixing the lyophilized powder with radix astragali polysaccharide, fructus Lycii polysaccharide, Glycyrrhrizae radix polysaccharide, herba Houttuyniae, Coptidis rhizoma, and poplar flower.
8. Use of a feed additive according to claim 1 for the preparation of a feed for combating bacterial diseases in pigs.
9. The use according to claim 8, wherein the feed additive is added in an amount of 3-7% by weight based on the total weight of the feed.
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| CN108077593B (en) * | 2017-11-30 | 2021-06-01 | 厦门百拓生物工程有限公司 | Antibiotic-free piglet concentrated feed |
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| CN111905019A (en) * | 2020-09-09 | 2020-11-10 | 张世淇 | Traditional Chinese medicine formula for regulating intestinal tracts of cultured animals and preparation method thereof |
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