CN108041299A - A kind of nonreactive compound feed for piglets - Google Patents
A kind of nonreactive compound feed for piglets Download PDFInfo
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- CN108041299A CN108041299A CN201711237840.XA CN201711237840A CN108041299A CN 108041299 A CN108041299 A CN 108041299A CN 201711237840 A CN201711237840 A CN 201711237840A CN 108041299 A CN108041299 A CN 108041299A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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Abstract
A kind of nonreactive compound feed for piglets.Major technique of the present invention belongs to biological technical field, relates generally to small-molecule substance glycine and glucose.Glycine can improve bacterium with glucose combination includes susceptibility of the drug-fast bacteria to antibiotic, so as to overcome bacterial resistance sex chromosome mosaicism.The present invention is combined with the small-molecule substance glycine, glucose and exocellular polysaccharide, the compound feed for piglets without any antibiotic being configured to again with other functional feedstuff additives and feedstuff, pass through the synergy of glycine, glucose and exocellular polysaccharide, improve piglet immunity of organisms and body function, when disease occurs in the piglet stage to be needed to prevent and prevent, bacterium is improved to preventing and the sensibility of prevention antibiotic, achievees the purpose that prevention and prevention bacterium include drug-fast bacteria harm.
Description
Technical field
Major technique of the present invention belongs to biological technical field, is the combination animal feed of a multidisciplinary field integrated application
Product, in particular to a kind of nonreactive compound feed for piglets.
Background technology
Antibiotic is used as feed medicated premix in existing routine compound feed for piglets, while uses five inorganic water
The metal trace elements such as copper sulphate, ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate feed addictive and Phos are supported
The feeding conventional young pig's mixed feed of Long term Animal is grown, the potential hazard for causing antibiotic resistance is not digested and assimilated with excessive
Antibiotic with problem of environmental pollution caused by waste.In addition, largely using micro members of inorganic metals such as copper and iron zinc-manganese in feed
Plain feed addictive and Phos, animal body be not high to their digestibility, the trace element that part is not digested and assimilated
It is excreted with Phos through excretory system, the environment that the fertilizer or processed material that waste is formed cause soil and groundwater etc. is dirty
Dye.
It is essential that although the protection of human health and life and animal intensive culture are played in the use of antibiotic
Effect, but due to the abuse of antibiotic and its misuse, and as threatening human health, livestock and poultry cultivation and aquaculture and life
The key factor of state environment.Therefore, control bacteria antibiotic drug resistance particularly significant.
At present, antibiotic largely uses in livestock breeding industry.On the one hand, some antibiotic control bacillary as veterinary drug
It is essential to infect.On the other hand, some antibiotic can promote growth of animal as feed medicated premix.Antibiotic it is big
Amount use makes sensitive bacteria mortality, and drug-fast bacteria is able to amount reproduction, promotes and enhance the drug resistance of bacterium.Variety classes resists
The use of raw element promotes the generation of multi-drug resistant bacteria, that is, generating can be with the bacterial strain of 3 kinds of drug resistance or more antibiotic.Control these
The infection of multi-drug resistant bacteria generally requires to replace antibiotics and increases antibiotic dosage.However, such control method is often
The Antibiotic Resistance for making the multi-drug resistant bacteria of remaining is wider, and drug resistance ability is stronger.Therefore, the few new technology with or without antibiotic of invention
Product is of great significance.
In the 1950s, due to the significantly immunocompetent discovery of polysaccharide, scholars are gradually from fungi, seaweed, high
Find that it has unique bioactivity in the polysaccharide obtained in the organisms such as plant, wherein with the promotion of polysaccharide and recovering body
Immunity function is especially prominent.Polysaccharide has antibacterial, antiviral, anti parasitic, resists as a kind of Immune enhancement and conditioning agent
The functions such as tumour, radioresistance, anti-aging.Active polysaccharide is to derive from a wealth of sources, cheap, definite effect, pure natural, and is subject to people
Most attention and research.Its application range is growing.
At present, glycine increases the nutritive additive of amino acid mainly as the feed that livestock and poultry particularly pet etc. eats
With attractant.In the feed in piglet stage, organic chelate is formed with copper and iron zinc-manganese in a manner of chelating there are glycine, is improved
Cultivated animals are to the digestibility of copper and iron zinc-manganese, to reduce the usage amount of copper and iron zinc-manganese.So far, no glycine promotes anti-
Raw element inhibits drug-fast bacteria growth and glycine and glucose combination promotes the report of antibiotic effect, also without glycine, grape
The report that sugar is used in combination with exocellular polysaccharide in compound feed for piglets.
The content of the invention
It is an object of the invention to provide a kind of raising bacterium to preventing and the sensibility of prevention antibiotic, pre- bacteriological protection
Include the young pig's mixed feed of drug-fast bacteria harm.
To realize the purpose of above-mentioned technology, products application solution of the invention is：
A kind of nonreactive compound feed for piglets does not contain any feed medicated premix, is containing weight percent
The glycine of 0.001%-5.0%, glucose 0.01%-5.0% are the extracellular more of 0.01%-5.0% containing weight percent
Sugar.
Further, a kind of nonreactive compound feed for piglets, the ingredient containing following weight percent：Glycine
0.001%-5.0%；Glucose 0.05%-5.0%；Exocellular polysaccharide 0.01%-5.0%；Calcium dihydrogen phosphate 0.1%-1.5%；
Mountain flour 0.1%-1.2%；Salt 0.1%-0.6%；Organic copper agent 0.01%-0.2%；Organic iron formulation 0.01%-
0.2%；Organic zinc preparation 0.02%-0.2%；Organic Manganese preparation 0.02%-0.2%；Organic trace element preformulation 0.01%-
3.0%；Lysine 0.1%-0.6%；Methionine 0.02%-0.5%；Threonine 0.02%-0.6%；Multidimensional 0.01%-
0.05%；Glycine betaine 0.01-0.2%；Sweetener 0.01-0.03%；Antioxidant 0.01-0.3%；Mould inhibitor 0.01%-
0.3%；Enzyme preparation 0.01%-0.2%；High-grade maize 30%-70.0%:；43% dregs of beans 2%-30.0%；46% dregs of beans
2%-30.0%；Soybean oil 1%-4.0%.
Further, a kind of nonreactive compound feed for piglets, the also ingredient containing following weight percent：Calcium monohydrogen phosphate
0-2.0%；Choline 0-1.5%；Tryptophan 0-0.5%；Phytase 0-2.0%；Acidulant 0-2%；Good quality wheat 0-20.0%；
High-quality barley 0-20.0%；Fermented bean dregs 0-15.0%；Popcorn 0-10.0%；Expanded soybean 0-20.0%；Whey powder 0-
8.0%；Imported fish meal 0-5.0%；White sugar 0-3.0%；Soybean oil 0.01-4.0%；Medium-height grass drug carrier 0-5.0%.
Further, a kind of nonreactive compound feed for piglets, it is characterised in that：The medium-height grass drug carrier refers to dried orange peel, mountain
Short, bristly hair or beard, cloves, origanum oil etc. have the plant deep processing intermixture of antibacterial and attractant effect.
Further, a kind of nonreactive compound feed for piglets, it is characterised in that：The glycine purity up to 99% with
On.
Further, a kind of nonreactive compound feed for piglets, it is characterised in that：The glucose is Dextrose Monohydrate,
Purity is up to more than 99.8%.
Further, a kind of nonreactive compound feed for piglets, it is characterised in that：The exocellular polysaccharide is with immune increasing
The carbohydrate pretended, including one or more of Microbial exopolysaccharides or plant exocellular polysaccharide.
Further, a kind of nonreactive compound feed for piglets, it is characterised in that：Make in piglet stage Direct-fed piglet
With.
Glycine and the collaboration of glucose both small-molecule substances make in use, antibiotic can be promoted to enter in bacterial body
Antibiotic content increase is more notable in bacterial body, and can improve the clinical drug-resistant bacterium of various bacteria and bacterium to kanamycins, soil
The sensibility of the antibiotic such as mycin, fortimicin, Amoxicillin.
In conclusion the technology of present invention tool initiative is using glycine, glucose and exocellular polysaccharide as nonreactive piglet
The core functionality additive raw material of mixed feed is combined with other nutrients and feedstuff, according to modem animal trophic model
Reasonably combined, science combination application is configured to a kind of nonreactive compound feed for piglets product, passes through the sweet ammonia of small-molecule substance therein
Acid is used with glucose compounding in nonreactive compound feed for piglets is prepared, and passes through the joint of glycine, glucose and exocellular polysaccharide
Effect improves piglet immunity of organisms and body function, improves sensibility of bacteria on antibiotic, reaches prevention and prevention bacterium bag
Include the purpose of drug-fast bacteria harm.Feed medicated premix (antibiotic) is used in compound feed for piglets for a long time as anti-than existing
In the application of bacterial drug resistance drug, there is higher wholesomeness and security.Pass through the combination to organic metal element simultaneously
Using and piglet nutrition balance, greatly reduce the use of the metal trace elements feed addictive such as copper and iron zinc-manganese and Phos
Amount, the metal trace elements such as copper and iron zinc-manganese and Phos pollution on the environment are not digested and assimilated so as to reduce in feed.
Description of the drawings
Fig. 1 is addition glycine and glucose can promote the result of study into antibiotic content in bacterial body.
Fig. 2 is glycine and/or glucose to improving result of study of the staphylococcus aureus to kanamycins sensibility.
Fig. 3 is glycine and/or glucose to improving result of study of the pseudomonas aeruginosa to kanamycins sensibility.
Fig. 4 is glycine and/or glucose to improving research of the Escherichia coli clinical drug-resistant bacterium to kanamycins sensibility
As a result.
Fig. 5 is glycine and/or glucose to improving result of study of the vibrio alginolyticus to kanamycins sensibility.
Fig. 6 can improve result of the Escherichia coli to the sensibility of terramycin for addition glycine and/or glucose.
Fig. 7 is Escherichia coli clinic bacterium drug result.
Fig. 8 is that addition glycine and/or glucose can cooperate with the sensibility for improving Escherichia coli clinic bacterium to terramycin
As a result.
Fig. 9 can improve research knot of the Wdwardsiella tarda to fortimicin sensibility for addition glycine and/or glucose
Fruit.
Figure 10 can improve result of study of the Escherichia coli to fortimicin sensibility for addition glycine and/or glucose.
Figure 11 can improve research of the Escherichia coli clinic bacterium to fortimicin sensibility for addition glycine and/or glucose
As a result.
Figure 12 can improve result of the Escherichia coli to the sensibility of Amoxicillin for addition glycine and/or glucose.
Figure 13 is Escherichia coli clinic bacterium drug result
Figure 14 is that addition glycine and/or glucose can cooperate with and improve sensibility of the Escherichia coli clinic bacterium to Amoxicillin
Result.
Specific embodiment
With reference to specific embodiment, the present invention is further explained.It is to be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.
Embodiment 1
Glycine and glucose can increase antibiotic and enter the in vivo quantity of bacterium
Bacterial death is related with into the antibiotic quantity inside bacterium.It is to study glycine and glucose to promoting antibiosis
Element is inoculated in 5mLLB culture mediums into the effect inside bacterium from picking Wdwardsiella tarda EIB202 single bacterium colonies on LB tablets
In, 30 DEG C of 200rpm shaken cultivations reach saturation state for 24 hours.Be collected by centrifugation bacterium solution, 8000rpm centrifugation 5min, remove supernatant and with
0.85% brine thalline finally with 1 × M9 (acetate containing 10mM) suspension thalline, adjusts bacterium solution OD values to 0.2, so
It is spare as test sample in test tube to dispense 5mL respectively afterwards.Experiment is divided into 5 groups, wherein 2 groups are control group, respectively not
Add any substance and addition antibiotic；Another 3 groups are experimental group, in addition antibiotic, add glycine, grape respectively
Sugar, glycine and glucose.After 6h being incubated in 30 DEG C of 200rpm shaking tables.Eccentric cleaning thalline, ultrasonic disruption use kanamycins
ELISA detection kit (Beijing Clover Technology Co., Ltd., Clover Technology Group Inc), which measures, blocks that
Mycin content.The result is shown in Figure 1.After adding glycine, 6.57 times are added than entering antibiotic in bacterial body during only added with antibiotic,
After adding glucose, internal antibiotic content adds 4.74 times, and after being with the addition of glucose and glycine, contain into antibiotic
Amount increases substantially, and adds 13.21 times.Illustrate to show when glycine and glucose both small-molecule substances collaboration use
It writes and improves into the in vivo antibiotic content of bacterium.
Embodiment 2
Glycine and glucose can improve sensibility of the various bacteria to kanamycins antibiotic
Picking various bacteria：Staphylococcus aureus (S.aureus), Pseudomonas aeruginosa (pseudomonas aeruginosa,
P.aeruginosa), Escherichia coli clinical drug-resistant bacterium (Y15), vibrio alginolyticus (V.alginolyticus) monoclonal to 100ml
In LB fluid nutrient mediums, 37 DEG C or 30 DEG C of 200rpm cultures 16h reach saturation state.Collect 20ml bacterium solutions, 8000rpm centrifugations
5min removes supernatant and with isometric 0.85% brine thalline, finally with 1 × M9 (acetate containing 10mM) suspended bacteria
Body adjusts bacterium solution OD to 0.2, then dispenses 5mL respectively in test tube, to add kanamycins as control group, then adds respectively
20mM glycine, 10mM glucose, 20mM glycine and 10mM glucose are experimental group, in 37 DEG C or 30 DEG C of 200rpm shaking tables
After being incubated 6h, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, the result is shown in Fig. 2-Fig. 5.For golden yellow it can be seen from these results
Staphylococcus (Fig. 2), after adding 20mM glycine and 10mM glucose respectively, germicidal efficiency be respectively increased 16.38 times and
32.75 times, and after adding 20mM glycine and 10mM glucose simultaneously, germicidal efficiency improves 327.5 times；It is false single for verdigris
Born of the same parents bacterium (Fig. 3), after adding 20mM glycine and 10mM glucose respectively, 1.97 times and 1.71 times have been respectively increased in germicidal efficiency,
And after adding 20mM glycine and 10mM glucose simultaneously, germicidal efficiency improves 20.99 times；For Escherichia coli clinical drug-resistant
Bacterium (Fig. 4), after adding 20mM glycine and 10mM glucose respectively, 1.05 times and 34.86 times have been respectively increased in germicidal efficiency,
And after adding 20mM glycine and 10mM glucose simultaneously, germicidal efficiency improves 305 times；For vibrio alginolyticus (Fig. 5), divide
Tian Jia not be after 20mM glycine and 10mM glucose, 1.3 times and 72.75 times have been respectively increased in germicidal efficiency, and add simultaneously
After 20mM glycine and 10mM glucose, germicidal efficiency improves 646.67 times.These the result shows that be with the addition of glycine respectively
After glucose, the germicidal efficiency that bacterium includes drug-fast bacteria is all improved, and after being with the addition of glycine and glucose simultaneously, sterilization
Efficiency is significantly improved, and illustrates that glycine and glucose compounding combination can improve sensitivity of the various bacteria to kanamycins
Property.
Embodiment 3
Glycine and/or glucose improve the sensibility of Escherichia coli and its clinical drug-resistant bacterium bacterium to terramycin
(1) glycine and/or glucose improve sensibility of the Escherichia coli to terramycin
The preparation of coli test sample：5 milliliters of LB cultures are inoculated in from picking Escherichia coli single bacterium colony on LB tablets
In base, saturation state is reached when 37 DEG C of 200rpm shaken cultivations 16 are small.It is collected by centrifugation bacterium solution, 8000rpm centrifugation 5min, in removing
Clearly and with 0.85% brine thalline, finally with 1 × M9 (acetate containing 10mM) suspension thalline, bacterium solution OD values are adjusted extremely
0.2, it is spare in test tube that 5mL is then dispensed respectively.
Ready sample is divided into 5 groups, wherein 2 groups are control group, any substance are not added respectively and addition soil is mould
Element；Another 3 groups are experimental group, in addition terramycin, add glycine, glucose, glycine and glucose respectively.37℃
After being incubated 6h in 200rpm shaking tables, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, the result is shown in Fig. 6.It can be seen from the results that with only adding
Terramycin is added to compare, after adding 20mM glycine and 10mM glucose respectively, 3.78 times of (existence have been respectively increased in germicidal efficiency
Rate by only add the 16.38% of terramycin fall to after addition terramycin and glycine 4.33%) and 4.85 times (under survival rate
3.38% be reduced to after addition terramycin and glucose), and after adding 20mM glycine and 10mM glucose simultaneously, germicidal efficiency
11.18 times (survival rate falls to 1.47% after addition terramycin and glucose and glycine) are improved, by adding sweet ammonia
After acid and/or glucose, Escherichia coli survival rate when being handled with terramycin is decreased obviously, and illustrates that both substances can improve
Escherichia coli are to the sensibility of terramycin, and with synergistic effect.
(2) glycine and/or glucose improve sensibility of the Escherichia coli clinical drug-resistant bacterium to terramycin
The measure of Escherichia coli clinical drug-resistant bacterium drug resistance：Escherichia coli are main in animal intestinal tract and quantity is most
A kind of bacterium, at present from clinically isolated mostly multi-drug resistant bacteria.From pig farm, separation obtains one plant of Escherichia coli, and
Its drug resistance is determined.As a result (Fig. 7) shows that this plant of bacterium is 625 micrograms/milli to the minimum inhibitory concentration of roxithromycin
Rise, be 6250 mcg/mls to the minimum inhibitory concentration of tetracycline, to the minimum inhibitory concentration of gentamicin for 2500 micrograms/
Milliliter, the minimum inhibitory concentration to clindamycin are 25000 mcg/mls, and the minimum inhibitory concentration to cefotaxime is 0.488
Mcg/ml, the minimum inhibitory concentration to Balofloxacin is 62.5 mcg/mls, to the minimum inhibitory concentration of ampicillin
For 6250 mcg/mls, the minimum inhibitory concentration to amikacin is 2500 mcg/mls, shows that Escherichia coli clinic bacterium is
One multi-drug resistant bacteria.
Glycine and/or glucose improve Study of Sensitivity of the Escherichia coli clinical drug-resistant bacterium to terramycin：It will be ready to
Sample (method according to above-mentioned Escherichia coli prepares experiment sample) be divided into 5 groups, wherein 2 groups be control group, do not add respectively
Add any substance and addition terramycin；Another 3 groups are experimental group, in addition terramycin, add glycine, grape respectively
Sugar, glycine and glucose.After being incubated 6h in 37 DEG C of 200rpm shaking tables, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, the result is shown in figures
8.It can be seen from the results that compared with only adding terramycin, after adding 20mM glycine and 10mM glucose respectively, germicidal efficiency
Being respectively increased 1.32 times, (survival rate falls to addition terramycin and glycine by only adding the 72.95% of terramycin
55.22%) and 1.6 times (survival rate falls to the 45.68% of addition terramycin and glucose), and the sweet ammonia of 20mM is added simultaneously
After acid and 10mM glucose, germicidal efficiency improves 2.73 times, and (survival rate falls to addition terramycin and glycine and grape
The 26.58% of sugar), after adding glycine and/or glucose, Escherichia coli clinical drug-resistant bacterium gives birth to when being handled with terramycin
The rate of depositing is decreased obviously, and illustrates that both substances can improve sensibility of the Escherichia coli clinical drug-resistant bacterium to terramycin, and has
Synergistic effect.
Embodiment 4
Glycine and/or glucose improve sensibility of the bacterium to fortimicin
(1) glycine and/or glucose can improve sensibility of the Wdwardsiella tarda to fortimicin
Ready sample is divided into 5 groups, wherein 2 groups are control group, does not add any substance and addition strength respectively
Mycin；Another 3 groups are experimental group, in addition fortimicin, add glycine, glucose, glycine and glucose respectively.
After being incubated 6h in 30 DEG C of 200rpm shaking tables, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, the result is shown in Fig. 9.It can be seen from the results that with
Only addition fortimicin compares, and after adding 20mM glycine and 10mM glucose respectively, germicidal efficiency has been respectively increased 5.97 times
(survival rate by only add the 96.61% of fortimicin fall to addition fortimicin and glycine 16.19%) and 7.08 times
(survival rate falls to the 13.64% of addition fortimicin and glucose), and 20mM glycine and 10mM glucose are added simultaneously
Afterwards, germicidal efficiency improves 11.18 times (survival rate falls to the 8.64% of addition fortimicin and glucose and glycine).
(2) glycine and/or glucose can improve sensibility of the Escherichia coli to fortimicin
Ready sample is divided into 5 groups, wherein 2 groups are control group, does not add any substance and addition strength respectively
Mycin；Another 3 groups are experimental group, in addition fortimicin, add glycine, glucose, glycine and glucose respectively.
After being incubated 6h in 37 DEG C of 200rpm shaking tables, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, the result is shown in Figure 10.It can be seen from the results that with
Only addition fortimicin compares, and after adding 20mM glycine and 10mM glucose respectively, germicidal efficiency has been respectively increased 1.49 times
(survival rate by only add the 99.71% of fortimicin fall to after addition fortimicin and glycine 67.24%) and 2.96
Times (survival rate falls to 33.62% after addition fortimicin and glucose), and adds 20mM glycine and 10mM Portugals simultaneously
After grape sugar, germicidal efficiency improves 4.09 times (after survival rate falls to addition fortimicin and glycine and glucose
24.42%).
(3) glycine and/or glucose can improve sensibility of the Escherichia coli clinic bacterium to fortimicin
Ready sample is divided into 5 groups, wherein 2 groups are control group, does not add any substance and addition strength respectively
Mycin；Another 3 groups are experimental group, in addition fortimicin, add glycine, glucose, glycine and glucose respectively.
After being incubated 6h in 37 DEG C of 200rpm shaking tables, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, the result is shown in Figure 11.It can be seen from the results that with
Only addition fortimicin compares, and after adding 20mM glycine and 10mM glucose respectively, germicidal efficiency has been respectively increased 1.35 times
(survival rate by only add the 96.59% of fortimicin fall to after addition fortimicin and glycine 71.59%) and 1.41
Times (survival rate falls to 68.4% after addition fortimicin and glucose), and adds 20mM glycine and 10mM grapes simultaneously
After sugar, germicidal efficiency improves 2.33 times (after survival rate falls to addition fortimicin and glycine and glucose
41.4%).
After adding glycine and/or glucose, various bacteria includes Wdwardsiella tarda, Escherichia coli and large intestine
Bacillus clinical drug-resistant bacterium survival rate when being handled with fortimicin is decreased obviously, and illustrates that both substances can improve these bacteriums
To the sensibility of fortimicin, and with synergistic effect.
Embodiment 5
Glycine and/or glucose can improve the sensibility of Escherichia coli and its Escherichia coli clinic bacterium to Amoxicillin
(1) glycine and/or glucose can improve sensibility of the Escherichia coli to Amoxicillin
The preparation of test sample：It is inoculated in from picking Escherichia coli single bacterium colony on LB tablets in 5 milliliters of LB culture mediums, 37 DEG C
Saturation state is reached when 200rpm shaken cultivations 16 are small.Be collected by centrifugation bacterium solution, 8000rpm centrifugation 5min, remove supernatant and with
0.85% brine thalline finally with 1 × M9 (acetate containing 10mM) suspension thalline, adjusts bacterium solution OD values to 0.2, then
It is spare in test tube that 5mL is dispensed respectively.
Ready sample is divided into 5 groups, wherein 2 groups are control group, does not add any substance and addition Ah not respectively
XiLin；Another 3 groups are experimental group, in addition Amoxicillin, add glycine, glucose, glycine and glucose respectively.
After being incubated 6h in 37 DEG C of 200rpm shaking tables, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, the result is shown in Figure 12.It can be seen from the results that with
Only addition Amoxicillin is compared, and after adding 20mM glycine and 10mM glucose respectively, germicidal efficiency has been respectively increased 9.36 times
(survival rate falls to the 2.7% of addition Amoxicillin and glycine by only adding the 25.29% of Amoxicillin) and 9.56 times of (lifes
The rate of depositing falls to the 2.64% of addition Amoxicillin and glucose), and after adding 20mM glycine and 10mM glucose simultaneously, it kills
Bacterium efficiency improves 18.55 times (survival rate falls to the 1.36% of addition Amoxicillin and glycine and glucose).
(2) glycine and/or glucose can improve sensibility of the Escherichia coli clinic bacterium to Amoxicillin
The measure of Escherichia coli clinical drug-resistant bacterium drug resistance：Escherichia coli are main in animal intestinal tract and quantity is most
A kind of bacterium, at present from clinically isolated mostly multi-drug resistant bacteria.From pig farm, separation obtains one plant of Escherichia coli, and
Its drug resistance is determined.As a result (Figure 13) shows that this plant of bacterium is 625 micrograms/milli to the minimum inhibitory concentration of roxithromycin
Rise, be 6250 mcg/mls to the minimum inhibitory concentration of tetracycline, to the minimum inhibitory concentration of gentamicin for 2500 micrograms/
Milliliter, the minimum inhibitory concentration to clindamycin are 25000 mcg/mls, and the minimum inhibitory concentration to cefotaxime is 0.488
Mcg/ml, the minimum inhibitory concentration to Balofloxacin is 62.5 mcg/mls, to the minimum inhibitory concentration of ampicillin
For 6250 mcg/mls, the minimum inhibitory concentration to amikacin is 2500 mcg/mls, shows that Escherichia coli clinic bacterium is
One multi-drug resistant bacteria.
Glycine and glucose can improve Study of Sensitivity of the Escherichia coli clinic multi-drug resistant bacteria to Amoxicillin：By standard
The sample got ready is divided into 5 groups (using the same sample preparation methods of above-mentioned Escherichia coli), wherein 2 groups are control group, is respectively
Any substance and addition Amoxicillin are not added；Another 3 groups are experimental group, in addition Amoxicillin, add sweet ammonia respectively
Acid, glucose, glycine and glucose.After being incubated 6h in 37 DEG C of 200rpm shaking tables, 100 μ L bacterium solutions is taken to carry out bacterium colony counting, knot
Fruit sees Figure 14.It can be seen from the results that compared with only adding Amoxicillin, 20mM glycine and 10mM glucose are added respectively
Afterwards, germicidal efficiency be respectively increased 5.06 times (survival rate by only plus Amoxicillin 89.32% fall to addition Amoxicillin and
The 17.64% of glycine) and 7.55 times (survival rate falls to the 11.82% of addition Amoxicillin and glucose), and add simultaneously
After adding 20mM glycine and 10mM glucose, germicidal efficiency improve 13.64 times (survival rate fall to addition Amoxicillin and
The 6.55% of glucose and glycine).
After adding glycine and/or glucose, survival rate is remarkably decreased when Escherichia coli are handled with Amoxicillin, is said
Bright glycine and/or glucose can improve sensibility of the Escherichia coli to Amoxicillin, and with synergistic effect.
Embodiment 6
Use of the nonreactive compound feed for piglets in the piglet cradling stage is tested
Test objective
For the pig farm that temperature relatively low season and the temperature difference are larger, the piglet cradling stage, there is the shapes of respiratory tract mixed infection
Condition and possible, the interim antibiotic combinations prevention disease for being directed to piglet and carrying out breathing problem, this examination usually during pig raising
Verify the nonreactive compound feed for piglets only containing glycine, glucose and exocellular polysaccharide and without any antibiotic in the piglet stage
The effect for carrying out disease prevention and improving weaned piglets is combined with feed medicated premix (antibiotic).
Test method
1st, animal selection and grouping：Experiment early-stage preparations are proceeded by from piglet is born the wean of 25 ages in days.Selection experiment
Pig farm is normal by the pig farm from numerous 30 piglets given birth to of sow through producing triplet, the 4th tire same period similar time rutting
Rule way is jumped from obstetric table to conservation pigsty, and piglet after wean is randomly divided into 16 columns, per column 18-20 heads, is born from piglet
35 ages in days select the similar piglet of the close health status of 10 column weight, are divided into 5 groups, 12 column of group adds up to 36-38 heads.
2nd, test process and daily ration composition：Test 5 processing groups of piglet point：Control group and test group 1, test group 2, experiment
Group 3, test group 4.Feed diet used in 5 groups is as follows：
Control group feed diet, weight composition are：Contain 20% oxytetracycline calcium in the every 1kg products of Product labelling mark
The commercially available piglet additive premix 10% of 500mg and 50% kitasamycin pulvis 100mg, high-grade maize 47.0%,
Popcorn 10.0%, expanded soybean 8.0%, 46% dregs of beans 11.0%, fermented bean dregs 6%, whey powder 3.0%, imported fish meal
2.0%th, white sugar 2.0%, soybean oil 1.0% add up to 100%.More than daily ration adds prevention 10% Amoxicillin of veterinary drug per 1kg
Pulvis 1000mg, 20% fortimicin pulvis 500mg stir evenly rear continuous use 7 days with feed.
A kind of 1 group of nonreactive compound feed for piglets for using this patent is tested, weight composition is：Glycine 5.0%, grape
Sugar 0.05%, exocellular polysaccharide 0.01%, calcium monohydrogen phosphate 0.9%, mountain flour 0.8%, salt 0.4%, have calcium dihydrogen phosphate 0.1%
Machine copper agent 0.16%, organic iron formulation 0.15%, organic zinc preparation 0.12%, Organic Manganese preparation 0.12%, organic micro member
Plain preformulation 0.2%, lysine 0.25%, methionine 0.11%, threonine 0.18%, tryptophan 0.01%, multidimensional
0.02%th, glycine betaine 0.02%, choline 0.05%, sweetener 0.01%, antioxidant 0.1%, mould inhibitor 0.1%, enzyme preparation
0.04%th, acidulant 0.1%, high-grade maize 50.0%, popcorn 10.0%, expanded soybean 10.0%, 46% dregs of beans
6.0%th, fermented bean dregs 6.0%, soy protein concentrate 6.0%, white sugar 2.0%, soybean oil 1.0% add up to 100%.
Test 1 group of feed diet：The nonreactive compound feed for piglets 1000kg of above 1 group of experiment, in addition prevention feed medicine
20% oxytetracycline calcium pulvis 500g of object additive, 50% kitasamycin pulvis 100g, rear continuous use 7 is stirred evenly with feed
My god.
Using a kind of nonreactive compound feed for piglets of this patent, weight forms is test group 2：Glycine 0.5%, grape
It is sugar 2.0%, exocellular polysaccharide 0.2%, calcium dihydrogen phosphate 0.4%, calcium monohydrogen phosphate 0.6%, mountain flour 0.6%, salt 0.1%, organic
Copper agent 0.15%, organic iron formulation 0.13%, organic zinc preparation 0.13%, Organic Manganese preparation 0.1%, organic trace element are pre-
Preparation 3.0%, lysine 0.15%, methionine 0.05%, threonine 0.12%, tryptophan 0.07%, multidimensional 0.01%, courage
It is alkali 0.08%, sweetener 0.015%, antioxidant 0.2%, mould inhibitor 0.1%, enzyme preparation 0.02%, acidulant 0.1%, excellent
Matter corn 48.0%, popcorn 10.0%, expanded soybean 8.0%, 46% dregs of beans 11.0%, fermented bean dregs 5.0%, whey powder
2.0%th, imported fish meal 3.0%, white sugar 1.0%, soybean oil 2.0%, tangerine peel powder 1.195% add up to 100%.
2 feed diet of test group：The nonreactive compound feed for piglets 1000kg of above 2 groups of experiment, in addition prevention feed medicine
20% oxytetracycline calcium pulvis 500g of object additive, 50% kitasamycin pulvis 100g, rear continuous use 7 is stirred evenly with feed
My god.
Using a kind of nonreactive compound feed for piglets of this patent, weight forms is test group 3：Glycine 0.05%, Portugal
Grape sugar 5.0%, exocellular polysaccharide 1.0%, calcium dihydrogen phosphate 1.2%, mountain flour 0.4%, salt 0.2%, organic copper agent 0.14%,
Organic iron formulation 0.14%, organic zinc preparation 0.13%, Organic Manganese preparation 0.11%, organic trace element preformulation 0.02%,
Lysine 0.35%, methionine 0.02%, threonine 0.03%, multidimensional 0.035%, glycine betaine 0.06%, sweetener
0.02%th, antioxidant 0.1%, mould inhibitor 0.1%, enzyme preparation 0.02%, acidulant 0.2%, high-grade maize 48.0%, excellent
Matter wheat 10.0%, 46% dregs of beans 10.0%, 43% dregs of beans 15.0%, whey powder 3.0%, imported fish meal 2.0%, soybean oil
2.0%th, tangerine peel powder 0.675% adds up to 100%.
3 feed diet of test group：The nonreactive compound feed for piglets 1000kg of above 3 groups of experiment, in addition prevention veterinary drug
10% Amoxicillin pulvis 1000g, 20% fortimicin pulvis 500g stir evenly rear continuous use 7 days with feed.
Using a kind of nonreactive compound feed for piglets of this patent, weight forms is test group 4：Glycine 0.001%,
Glucose 0.01%, exocellular polysaccharide 5.0%, calcium dihydrogen phosphate 0.7%, calcium monohydrogen phosphate 0.2%, mountain flour 0.9%, salt 0.5%,
Organic copper agent 0.13%, organic iron formulation 0.16%, organic zinc preparation 0.1%, Organic Manganese preparation 0.09%, organic micro member
Plain preformulation 1.2%, lysine 0.45%, methionine 0.3%, threonine 0.45%, tryptophan 0.12%；Multidimensional 0.05%,
Glycine betaine 0.07%, sweetener 0.03%, antioxidant 0.2%, mould inhibitor 0.1%, enzyme preparation 0.02%, acidulant
0.219%th, high-grade maize 50.0%, popcorn 10.0%, expanded soybean 10.0%, 46% dregs of beans 11.0%, soybean concentration
Albumen 4.0%, white sugar 2.0%, soybean oil 2.0% add up to 100%.
4 feed diet of test group：The nonreactive compound feed for piglets 1000kg of above 4 groups of experiment, in addition prevention veterinary drug
10% Amoxicillin pulvis 1000g, 20% fortimicin pulvis 500g stir evenly rear continuous use 7 days with feed.
Control group and 1 group of experiment, 2 groups of experiment, 3 groups of experiment, 4 groups of experiment all start on the 5th day what experiment formally started
Prevention and prevention feed medicated premix (antibiotic) and veterinary drug shown on being separately subject to during compound feed for piglets mixture manufacturing,
And continuous use 7 days.
3. feeding management：5 group of 10 column feeding piglet is in the adjacent swinery of same building piglet house, the ground in addition to slatted floor
Face is heat insulation floor, free choice feeding, free water slot drinking-water, well-ventilated.Each group piglet takes the identical freely side of feeding
Formula.
4th, observe, record：Initial head number and beginning weight, the end of term head number and end of term weight of the front and rear record piglet of experiment, experiment
It is interim to pay attention to observation, record pig situation, abnormal pig timely processing.
Results and discussion
By the formal test of 30 days, the results are shown in Table 1：
1 result of the test of table counts
As can be seen from Table 1：
Test 1 group, 2 groups of experiment, 3 groups of experiment, 4 groups of experiment death rate during experiment less than control group be respectively 5.6,
2.6,2.7,2.8,10.8 (%)；
Test 1 group, experiment 2 groups, experiment 3 groups, experiment 4 groups between experimental period Average weight increasing a day be respectively higher than control group
354.5,395.7,365.0,388.2,266.4 (g/ days/head).
More than compliance test result test proves, 2 groups of experiment glycine of 1 group of piglet step-by-step test and experiment, glucose and extracellular
Polysaccharide and 20% oxytetracycline calcium pulvis and 50% kitasamycin pulvis are combined；Test 3 groups and experiment 4 groups of experiment glycine, grapes
Sugar and exocellular polysaccharide and 10% Amoxicillin pulvis and 20% fortimicin pulvis are combined, in prevention and the death of prevention piglet and really
It protects in production performance and is better than 20% oxytetracycline calcium, 50% kitasamycin pulvis, the 10% Amoxicillin pulvis and 20% of control group
The combination of fortimicin pulvis.Illustrate the nonreactive only containing glycine, glucose and exocellular polysaccharide and without any antibiotic
Compound feed for piglets is combined in the piglet stage by interim with suitable feed medicated premix and veterinary drug, can play raising
Sensibility of bacteria on antibiotic reaches and improves piglet immunity of organisms and body function, and prevention and prevention bacterium include drug-fast bacteria
The purpose of harm.
Claims (8)
- It is 0.001%- containing weight percent 1. a kind of nonreactive compound feed for piglets does not contain any feed medicated premix 5.0% glycine, glucose 0.01%-5.0% contain the exocellular polysaccharide that weight percent is 0.01%-5.0%.
- 2. a kind of nonreactive compound feed for piglets as described in claim 1, does not contain any feed medicated premix, containing following heavy Measure the ingredient of percentage：Glycine 0.001%-5.0%；Glucose 0.05%-5.0%；Exocellular polysaccharide 0.01%-5.0%；Biphosphate Calcium 0.1%-1.5%；Mountain flour 0.1%-1.2%；Salt 0.1%-0.6%；Organic copper agent 0.01%-0.2%；Organic iron formulation 0.01%- 0.2%；Organic zinc preparation 0.02%-0.2%；Organic Manganese preparation 0.02%-0.2%；Organic trace element preformulation 0.01%-3.0%； Lysine 0.1%-0.6%；Methionine 0.02%-0.5%；Threonine 0.02%-0.6%；Multidimensional 0.01%-0.05%；Glycine betaine 0.01- 0.2%；Sweetener 0.01-0.03%；Antioxidant 0.01-0.3%；Mould inhibitor 0.01%-0.3%；Enzyme preparation 0.01%-0.2%；It is excellent Matter corn 30%-70.0%；43% dregs of beans 2%-30.0%；46% dregs of beans 2%-30.0%；Soybean oil 1%-4.0%.
- 3. a kind of nonreactive compound feed for piglets as described in claim 1, the also ingredient containing following weight percent：Calcium monohydrogen phosphate 0-2.0%；Choline 0-1.5%；Tryptophan 0-0.5%；Phytase 0-2.0%；Acidulant 0-2%；Good quality wheat 0-20.0%；It is high-quality big Wheat 0-20.0%；Fermented bean dregs 0-15.0%；Popcorn 0-10.0%；Expanded soybean 0-20.0%；Whey powder 0-8.0%；Import fish Powder 0-5.0%；White sugar 0-3.0%；Soybean oil 0.01-4.0%；Medium-height grass drug carrier 0-5.0%.
- 4. a kind of nonreactive compound feed for piglets as claimed in claim 3, it is characterised in that：The medium-height grass drug carrier refers to dried orange peel, mountain Short, bristly hair or beard, cloves, origanum oil etc. have the plant deep processing intermixture of antibacterial and attractant effect.
- 5. a kind of nonreactive compound feed for piglets as described in claim 1, it is characterised in that：The glycine purity is up to more than 99%.
- 6. a kind of nonreactive compound feed for piglets as described in claim 1, it is characterised in that：The glucose is Dextrose Monohydrate, Purity is up to more than 99.8%.
- 7. a kind of nonreactive compound feed for piglets as described in claim 1, it is characterised in that：The exocellular polysaccharide is with immune increasing The carbohydrate pretended, including one or more of Microbial exopolysaccharides or plant exocellular polysaccharide.
- 8. a kind of nonreactive compound feed for piglets as described in claim 1, it is characterised in that：Make in piglet stage Direct-fed piglet With.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109090351A (en) * | 2018-08-25 | 2018-12-28 | 郑州智谷工业技术有限公司 | A kind of glucose oxidase mixed feed additive |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0361452A (en) * | 1989-07-31 | 1991-03-18 | Kazuo Uranaka | Mixed feed for livestock |
| US20070009502A1 (en) * | 2005-07-08 | 2007-01-11 | Rajiv Lall | Nutritional conjunctive support therapy for recovery in animals following stress or illness |
| CN101390578A (en) * | 2008-10-24 | 2009-03-25 | 厦门百拓生物工程有限公司 | Polysaccharide additive premixing feedstuff for pig |
| CN103432158A (en) * | 2013-08-27 | 2013-12-11 | 集美大学 | Polysaccharide compound for preventing and treating porcine diarrhea and application thereof |
| CN104041712A (en) * | 2014-06-23 | 2014-09-17 | 陕西石羊农业科技有限公司 | Creep compound feed applicable to 7-38-days-old piglets and preparation method of creep compound feed |
| CN104161181A (en) * | 2013-07-18 | 2014-11-26 | 河南联合英伟饲料有限公司 | Feed additive for preventing pig bacterial diseases, preparation method and application thereof |
| CN105248881A (en) * | 2015-11-10 | 2016-01-20 | 广东中农联生物制药有限公司 | Composite functional traditional Chinese medicine additive and preparation method thereof |
-
2017
- 2017-11-30 CN CN201711237840.XA patent/CN108041299B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0361452A (en) * | 1989-07-31 | 1991-03-18 | Kazuo Uranaka | Mixed feed for livestock |
| US20070009502A1 (en) * | 2005-07-08 | 2007-01-11 | Rajiv Lall | Nutritional conjunctive support therapy for recovery in animals following stress or illness |
| CN101390578A (en) * | 2008-10-24 | 2009-03-25 | 厦门百拓生物工程有限公司 | Polysaccharide additive premixing feedstuff for pig |
| CN104161181A (en) * | 2013-07-18 | 2014-11-26 | 河南联合英伟饲料有限公司 | Feed additive for preventing pig bacterial diseases, preparation method and application thereof |
| CN103432158A (en) * | 2013-08-27 | 2013-12-11 | 集美大学 | Polysaccharide compound for preventing and treating porcine diarrhea and application thereof |
| CN104041712A (en) * | 2014-06-23 | 2014-09-17 | 陕西石羊农业科技有限公司 | Creep compound feed applicable to 7-38-days-old piglets and preparation method of creep compound feed |
| CN105248881A (en) * | 2015-11-10 | 2016-01-20 | 广东中农联生物制药有限公司 | Composite functional traditional Chinese medicine additive and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109090351A (en) * | 2018-08-25 | 2018-12-28 | 郑州智谷工业技术有限公司 | A kind of glucose oxidase mixed feed additive |
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Denomination of invention: A compound feed for piglets without resistance Effective date of registration: 20210304 Granted publication date: 20201229 Pledgee: Postal Savings Bank of China Limited Changtai County sub branch Pledgor: FUJIAN NISERVE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021350000031 |