CN102885202A - Novel poultry micro-ecological preparation and preparation method thereof - Google Patents

Novel poultry micro-ecological preparation and preparation method thereof Download PDF

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Publication number
CN102885202A
CN102885202A CN2012103422824A CN201210342282A CN102885202A CN 102885202 A CN102885202 A CN 102885202A CN 2012103422824 A CN2012103422824 A CN 2012103422824A CN 201210342282 A CN201210342282 A CN 201210342282A CN 102885202 A CN102885202 A CN 102885202A
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vitamin
cobastab
parts
probiotics
rate
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马秋刚
来光明
计成
刘延岩
薛华伟
李卫东
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BEIJING FUWISH BIOTECHNOLOGY Co Ltd
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BEIJING FUWISH BIOTECHNOLOGY Co Ltd
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Abstract

The invention relates to a novel poultry micro-ecological preparation and a preparation method of the novel poultry micro-ecological preparation. The preparation comprises following components in part by weight: 0.8-3.5 parts of vitamin A, 0.2-0.8 part of vitamin D, 2-8 parts of vitamin E, 0.2-1.2 parts of vitamin K, 0.1-0.9 part of vitamin B1, 0.2-0.8 part of vitamin B2, 0.1-0.7 part of vitamin B6, 0.08-0.5 part of vitamin B12, 2-5 parts of nicotinamide, 0.8-3.5 parts of calcium pantothenate, 0.02-0.4 part of folic acid, 0.2-1.2 parts of biotin, 0.02-2 parts of vitamin C, 1.3-32 parts of enterococcus faecium, 27-155 parts of bacillus subtilis, 75-210 parts of oligosaccharide, 150-400 parts of pine needle meal and 400-600 parts of bentonite. The novel poultry micro-ecological preparation can improve daily gain, feed intake, feed conversation efficiency of young poultry, reduces death and culling rate of the young poultry, and improves reproductive performance of breeding poultry, laying rate, fertilization rate of hatching eggs, hatching rate, and healthy rate of the young poultry. According to the invention, as double-layer shallow coating is performed to enterococcus faecium, the stress resistance of enterococcus faecium is improved.

Description

A kind of novel fowl probiotics and preparation method thereof
Affiliated technical field
Patent of the present invention relates to the preparation method of probiotics, especially a kind of novel fowl preparation method of probiotics.
Background technology
The poultry cultivation of China has passed through the sustainable development of many decades, and the number of animals raised constantly increases, and quantum of output is also in continuous rising.2011, the poultry total output surpassed 1,600 ten thousand tons, and 2,811 ten thousand tons of birds, beasts and eggs total outputs increase by 1.8% than the last year.But even if China is the second-biggest-in-the-world production of poultry meat state that is only second to the U.S., and birds, beasts and eggs output accounts for more than 40% of Gross World Product, and the poultry cultivation level of China but still is in a kind of relatively original state.Our poultry consumption proportion is lower, and chicken consumption per head in 2011 only is 12kg, well below the 42.2kg of the U.S., the 40.6kg of Saudi Arabia and the 39.3kg of Brazil; Simultaneously, our poultry export volume is well below our poultry import volume.In recent ten years, our poultry outlet European Union has all suffered to besiege and chase, and European Union uses a series of TBT (Technical Barriers to Trade) to be means, raises poultry product import threshold, and the poultry that limits us enters EU market.In on May 24, August 1 to calendar year 2001 in 1996, contain animal epidemic and agricultural chemicals, residue of veterinary drug etc. in the poultry of European Union with Chinese exports and serve as reasons China is closed market.In January, 2002, European Union does not reach its requirement and serves as reasons to contain residual chloromycetin and Chinese trace standard of pesticide system from the Some Animals derived food of Chinese import, completely forbids from the animal derived product of Chinese import.Just at same year, " block and make " assigned to Chinese poultry product in succession by Korea S, Japan, Malaysia and Russia.Although again successively to the open poultry of Chinese Enterprise market, for the animal epidemic in our poultry, the problems such as agricultural chemicals, residue of veterinary drug needed us more to pay attention to since 2008 in these countries.
The paces of bird large-scale cultivation are constantly moving ahead, and the purpose of large-scale cultivation is to promote whole cultivation level, and are lower with green, science, efficiently cultivation means solution bird production performance, the problems such as agricultural chemicals, residue of veterinary drug.About the residue of veterinary drug problem, it all is the abuse that comes from feeding antibiotic that larger proportion is arranged.The poultry of China, egg production amount are huge, but most of product is for a long time all produced by small-sized raiser and provided.Large-scale cultivation degree in recent years improves gradually, just changes the small-scale raiser of professional large-scale farming field and sideline production and the situation of depositing into.Under this situation, cultivate very highly to the degree of dependence of manpower management and nursing, the level of raising is limited, and sanitary condition is not fully up to expectations especially, thereby the chance of blindly medication increases.Especially owing to cultivating improving constantly of intensive degree, stocking density is increasing, add that the modern varieties breeding technique excessively develops Production of Livestock and Poultry potential, cause the raiser in of short duration increasing economic efficiency, also to increase the various incidences that stress reach epidemic disease.These reasons cause the raiser to use more and more at the breeding process Chinese traditional medicine, progressively reach the degree that can't leave medicine in whole breeding process fully.
The risk problem of antibiotic resistance also still exists in the large-scale cultivation field, and at the beginning of this year, the Dutch consumers' association communique of releasing news says, on the Dutch market from 99% all having the antibiotic resistance risk in the chicken of intensive poultry farms.And the also comparatively serious antibiotics resistance problem of ubiquity of the various plants of China, pathogen isolated from clinical specimens has the trend that increases year by year to the resistant rate of the antibacterials such as tetracycline, kanamycins, streptomysin, gentamicin, Enrofloxacin, ampicillin.(2004), its equalitys of Zhao (2003) such as Zhang Zhengqing etc. (2007), Liu Shuanjiang are studied the drug resistance of fowl source salmonella drug resistance, chicken source Drug Resistance of E. coli, chicken coccidia medicine respectively.Result's demonstration, these three kinds of common germs have higher drug resistance to the above-mentioned antibacterials of enumerating.
2008, one piece of article that is published on Britain's " nature " weekly was pointed out, uses antibiotic might destroy the congenital immunity power of enteron aisle, and this also is the reason that causes that the antibiotic-resistant bacterium infects.Research also finds, although antibiotic can be eliminated a lot of bacteriums in the enteron aisle, the damage of the enteron aisle congenital immunity function that is caused by antibiotic therapy also provides hotbed for the breeding of antibiotic-resistant bacterium.Generally speaking, use for a long time antibiotic easily to produce drug resistance, cause feed medication and medicine for treatment amount to raise, even cause livestock and poultry to the irritated toxic reaction of antibiotic medicine; Simultaneously, antibiotic use descends Abwehrkraft des Koepers, and various bacillary and viral diseases easily occur; And the residual meeting of antibiotic in livestock products enters human body by food chain, and harm humans is healthy.
So far, there have been a plurality of countries to completely forbid gradually the use of feeding antibiotic, although China does not also enter the stage of using of completely forbidding, but consider from long-range angle, it is more and more higher that the consumer is forbidden antibiotic cry, pursuit for pollution-free, noresidue, publicly harmless animal product is also more ardent, and forbidding feeding antibiotic certainly will become the inevitable development trend of Animal Husbandry in China comprehensively.Ban use of after the feeding antibiotic; just must need to find a kind of antibiotic substitute of environmental protection; descend in order to breeding performonce fo animals decline, the immunity function that solves in the bird large-scale cultivation; and the problem such as epidemic prevention and control, alleviate the negative effect that the antibiotic forbidding brings Production of Livestock and Poultry.
At present, antibiotic substitute mainly can be summarized as five large classes: probiotics, enzyme preparation, functional oligosaccharide, organic acid preparation, Chinese herbal medicine extract.Wherein, probiotics move to maturity gradually, but still there are some problems in present existing probiotics when reality is used as a kind of comparatively popular antibiotic substitute of accepting.
The problem that existing probiotics mainly exists when using is: easy inactivation during 1. active bacteria formulation is processed, transported at feed; 2. after viable bacteria enters alimentary canal, mostly be difficult to stand the effect of hydrochloric acid in gastric juice, bile acid, low pH value, difficulty has enough quantity to reach enteron aisle or settles down enteron aisle and play a role; 3. the speed of growth is slow, be difficult to the competition of harmful enteric microorganism in the status of having the advantage; 4. bacterial classification is single, lacks compatibility research, and action effect is unilateral.Patent CN1965673 utilizes the Mixed Microbes of one or more compositions of Bacillus acidi lactici, bacillus, saccharomycete, filamentous fungi and photosynthetic bacteria, regulate the body microecological balance to reach, promote the purpose of the useful nutritional labelings such as intestinal absorption secretion multivitamin and amino acid.But the viable bacterias such as Bacillus acidi lactici are easy inactivation in feed processing, transportation, and are relatively poor to the tolerance of hydrochloric acid in gastric juice, bile acid, low pH value, and the effect during actual the use can be had a strong impact on.Patent CN 102178047A is that mixing in 1: 1: 1 forms with lactobacillus acidophilus, Lactobacillus casei and Lactobacillus plantarum by the viable count ratio, is used for alleviating the animal intestinal inflammation, and adjusting is immune, improves the growth of animal performance.These three kinds of lactobacillus are stronger for the tolerance of environment in the alimentary canal, produce sour effect better, but the bacterial classification classification are single, and effect is close, can't reach the effect of mutually replenishing, for improving the efficiency of feed utilization DeGrain.Patent 201010517542.8 forms a kind of probiotics with Miyarisan viable bacteria and acceptable carrier, Miyarisan can produce butyric acid, enzyme and vitamin, the ability that has good promotion nutrient digestion to absorb, but Miyarisan tolerance to cholate in enteron aisle is not yet clear, so the activity of Miyarisan in alimentary canal is still waiting research.
Summary of the invention
The purpose of patent of the present invention provides a kind of probiotics, with substitute antibiotics, alleviates the negative effect that the feeding antibiotic forbidding brings poultry production, thereby solves the problems referred to above that prior art exists.
The method for coating that patent employing patent 201010579636.8 of the present invention provides carries out double-deck shallow coated for protecting with the VREF of nucleus, and concrete operations implementation method referenced patent 201010579636.8 is carried out.
Patent of the present invention provides a kind of novel fowl probiotics, and it comprises following active ingredient by weight: vitamin A 0.2-4.5 part, vitamin D 0.2-0.9 part, vitamin E 2-8 part, vitamin K 0.1-1.2 part, Cobastab 10.05-0.9 part, Cobastab 20.2-1.2 part, Cobastab 60.01-0.8 part, Cobastab 120.03-0.6 part, niacinamide 1-6 part, calcium pantothenate 0.3-5 part, folic acid 0.02-0.9 part, biotin 0.05-1.4 part, vitamin C 0.01-2 part, VREF 1.2-38 part, bacillus subtilis 16-155 part, oligosaccharides 70-260 part, pine needle meal 150-400 part, bentonite 350-600 part.
Preferably, it comprises following active ingredient by weight: vitamin A 0.8-3.5 part, vitamin D 0.2-0.8 part, vitamin E 2-8 part, vitamin K 0.2-1.2 part, Cobastab 10.1-0.9 part, Cobastab 20.2-0.8 part, Cobastab 60.1-0.7 part, Cobastab 120.08-0.5 part, niacinamide 2-5 part, calcium pantothenate 0.8-3.5 part, folic acid 0.02-0.4 part, biotin 0.2-1.2 part, vitamin C 0.02-2 part, VREF 1.3-32 part, bacillus subtilis 27-155 part, oligosaccharides 75-210 part, pine needle meal 150-400 part, bentonite 400-600 part.
Fowl of the present invention also comprises the additive of following weight parts with probiotics: cyclodextrin 15-79 part, cellulose 9-28 part, converted starch 5-31 part, gelatin 2-23 part, anti-current powder 2-6 part, antistatic additive 2-6 part, antioxidant 0.5-20 part.
Described oligosaccharides is the mixture of FOS, oligomeric maltose and sucrose, and the weight ratio of three kinds of components is 5-80: 5-80: 5-60.
Described anti-current powder is silica.
Described antistatic additive is mineral white oil.
Described antioxidant is tert-butyl-5-methyl phenol and uncle's propyl group-tetrahydroxy anisole mixture, and weight ratio is 1: 1-3.
Patent of the present invention also provides the method for the above-mentioned novel fowl of preparation with probiotics, comprises the steps:
1, internal layer is coated
Equipment: fluidized bed granulation seed-coating machine
VREF is dropped in the drying tower.
By 100-500 part, 100-300 part, the ratio of 200-800 part is made into the 3-10% aqueous solution with the cellulose, converted starch, cyclodextrin that account for probiotics quality 2-10%.
Mentioned solution is pressed in the machine spray gun.Starting device is adjusted 60-100 ℃ of EAT, and the mode with high-pressure fog is sprayed onto on the VREF simultaneously, and approximately 1-2 hour, treat its drying, moisture is less than 7%.
2, outer coated
Equipment: fluidized bed granulation seed-coating machine
By 200-800 part, the ratio of 100-900 part is made into 2~20% coating buffer with the cyclodextrin, gelatin that account for probiotics quality 1-3%.
Mentioned solution is pressed in the machine spray gun.Starting device is adjusted 60~100 ℃ of EATs, and the mode with high-pressure fog is sprayed onto on above-mentioned steps 1 encrusting substance simultaneously, and approximately 2-3 hour, until drying materials.
3, by said ratio, with said vitamin monomer Folic Acid, calcium pantothenate, Cobastab 1, Cobastab 12, biotin, the bentonite of 1/10-1/5 amount is put into the mixer premix, incorporation time 5~10 minutes;
4, will fully mix 10~15 minutes in the product of step 2, step 3 and the remaining ingredient mixer, and get final product.
The active bacteria formulation of existing probiotics easy inactivation in feed processing, transportation after viable bacteria enters alimentary canal, is difficult to stand the effect of hydrochloric acid in gastric juice, bile acid, low pH value mostly, and difficulty has enough quantity to reach enteron aisle or settles down enteron aisle and play a role.Patent of the present invention is coated with the VREF that easily is subject to the various extraneous factor impacts in feed processing, the transportation in process of production, external environment is renderd a service the effect of viable bacteria to be reduced, guaranteed that this product has enough viable bacterias to reach enteron aisle, plays a role after entering alimentary canal.
Patent of the present invention further provides the using method of described probiotics, comprises the steps:
A: chick: 0-21 age in days, spice: 500 grams/ton mixed feed;
B: plant chicken: laying period, begin continuous use, spice: 500 grams/ton mixed feed;
Wherein, VREF (Enterococcusfaecium) belongs to a kind of in the enterococcus, is amphimicrobian lactic acid bacteria.It is not only normal bacterial classification common in the humans and animals enteron aisle, also is usually used in improving food, quality of the fodder.VREF is to be convenient to the first-selected bacterial classification producing and use with respect to strictly anaerobic, Bifidobacterium, the lactobacillus of cultivating the preservation condition harshness.1989, U.S. FDA was just announced it for can be directly used in one of bacterial classification of animal.
Li Xin etc. (2011) to 9 probiotics active variation in feed processing and storage process done a series of contrast, test and selected the bacillus of 5 strains and the lactic acid bacteria of 4 strains, wherein code name be SC be VREF.By comparing the situations such as feed mixing, feed granulating, powder storage, particulate material storage to the impact of 9 probiotics strain activity, can determine that 5 bacillus are fit to feed processing and storage.And in 4 strains of lactic acid bacteria, no matter VREF is in the situation that feed mixing, feed granulating, powder storage or particulate material are stored and all had the highest survival rate, and the survival effect significantly is better than lactobacillus acidophilus, streptococcus thermophilus, chicken enterococcus.
Used VREF (CECT410) can survive in SGF pH value 2.0 in the test of (2007) such as Nelson P é rez Guerra.Submergence can also normal growth after 120 minutes in the SGF of pH value 2.0 for VREF ANSE228 among the patent CN 201010539988.0.A large number of experiments show that, the VREF that obtains of screening can tolerate 1.0% gallbladder salinity, even up to 3.0% and the high salt of NaCl of 5%-6.5%.As seen, VREF has good stomach juice-resistant performance, and is larger by the possibility of stomach survival, and it has simultaneously good bile tolerance performance and to the tolerance of intestines and stomach against the property environment, has possessed the primary condition in the small intestine field planting.Enterococcus, especially VREF have drug resistance (Brunton, 1984 to broad-spectrum antibiotic; Murray, 1990; Woodford etc., 1995; Gold etc., 1996; Rice etc., 1996; Klare etc., 1997; Quintiliani etc., 1999; Kak etc., 2002; Klare etc., 2002).And evidence is arranged, in the time of 60 ℃, still can not examine viable bacteria through coated VREF.Great majority experiment showed, that the probiotic that is comprised of VREF can improve the body weight gains of livestock and poultry childhood. improve the price of deed.Reduce diarrhea rate, reduce the death rate.In addition, the VREF metabolism produces the materials such as organic acid, biacetyl, hydrogen peroxide, bacteriocin, and these materials have pathogen and spoilage organisms, the raising immunity of inhibition, improve the physiological function such as livestock products quality.
And but bacillus subtilis is the Direct-fed animal of China Ministry of Agriculture announcement and allows one of 16 kinds of feed grade probio bacterial classifications that use.Bacillus subtilis can stop growing under the condition of nutritional deficiency, under extreme condition, can also induce to produce the very strong endogenous spore of resistance, has the characteristics such as high temperature resistant, anti-extruding, resistance to oxidation, acid and alkali-resistance.In suitable environment, gemma can bring back to life again, and amount reproduction.
Owing to surviving in the multiple environment, bacillus subtilis has abundant product enzyme system, can utilize the multiple nutrients substance classes, can produce tens kinds of enzymes such as protease, amylase, 1,4 beta-glucanase, phytase, pectase and zytase.Bacillus subtilis can be converted into mycoprotein with the vegetable protein in the feed during the fermentation, is easilier absorbed by animal; Macromolecular albumen is converted into micromolecular little peptide, improves digestibility; Produce various enzymes, decomposition of cellulose etc., the ANFs of can also degrading simultaneously decomposes trypsin inhibitor, urase, phytolectin etc.The product enzyme effect of bacillus subtilis can promote enteron aisle to the absorption of nutriment, improves feed efficiency, reduces feedstuff-meat ratio.
Pan Kangcheng etc. (2005) studies show that, in the basal diet of broiler chicken, add 0.1% bacillus subtilis formulation after, net gain, the weight average that increases day by day are significantly increased; Feed intake and feed conversion rate also are significantly improved; Chicken meat quality and amino acid composition then have clear improvement.According to (2009) such as Xu Haiyan, adding bacillus in the diet has the function that promotes that chick thymus gland, spleen and the bursa of farbricius are grown, and can significantly improve IgG content in the serum, strengthens the anti-stress ability of animal.And compare with single use bacillus subtilis, many bacterial classifications cooperate therapy more comprehensive, effective.Simultaneously, because bacillus subtilis has very strong biological assimilation, therefore can be applicable to remove the ight soil stench of livestock and poultry farm, its metabolite also has inhibitory action to the growth and breeding of mosquitos and flies.
The probiotics of patent of the present invention has following beneficial effect:
1, the beneficial bacterium among the present invention can improve feed intake, daily gain and the feed conversion rate of chick, aspect the reduction death rate significant effect is being arranged.
2, the beneficial bacterium among the present invention can improve the reproductive performance of kind of chicken, plants the performances such as laying rate of chicken, fertility rate of hatching egg, incubation rate, strong young rate and all increases significantly.
3, the VREF among the present invention can fast breeding and carries out the occupy-place effect, sets up the dominant microflora of intestinal beneficial bacterium.Simultaneously, the VREF among the present invention can secrete lactic acid, suppresses breeding and the growth of pathogen, regulates the microecological balance of body enteron aisle before stress occuring, improve to stress resistivity, reduces sick of follow-up infection.
4, the bacillus subtilis among the present invention can promote the Development of Immune Organs of fowl, strengthens immunity of organisms; Produce enzyme and antibacterial peptide, suppress the pathogenic bacteria breeding; Improve poultry, birds, beasts and eggs product quality.
5, the oligosaccharides among the present invention can promote the Fast-propagation of beneficial bacterium, further enlarges the flora advantage of beneficial bacterium, and vitamin is improved anti-stress ability and shorten the treatment effect of the course for the treatment of, reduces case fatality rate and reduces the sick rate of washing in a pan.
Description of drawings
Fig. 1 is fowl of the present invention with the affect figure of probiotics on average daily gain;
Fig. 2 is fowl of the present invention with the affect figure of probiotics on kind of the chicken reproductive performance of laying eggs.
The specific embodiment
Following examples are used for explanation patent of the present invention, but are not used for limiting the scope of patent of the present invention.
Among the embodiment VREF, bacillus subtilis be to obtain from main fermentation, all the other raw material components are all commercial.
Embodiment 1
The probiotics of patent of the present invention comprises following component: vitamin A 1.7g, vitamin D 0.4g, vitamin E 2.9g, vitamin K 0.3g, Cobastab 10.12g, Cobastab 20.57g, Cobastab 60.12g, Cobastab 120.1g, niacinamide 1.4g, calcium pantothenate 0.7g, folic acid 0.1g, biotin 0.2g, VREF 29g, bacillus subtilis 110g, oligosaccharides 83g, pine needle meal 190g, bentonite 561.42g, tert-butyl-5-methyl phenol 6g, uncle's propyl group-tetrahydroxy anisole 6g, silica 3.17g, mineral white oil 4.5g, cyclodextrin 40g, cellulose 15g, converted starch 10g, gelatin 10g; Oligosaccharides comprises FOS 10g, oligomeric maltose 57g, sucrose 15g.
Follow these steps to preparation:
1) 15g cellulose, 10g converted starch, 25g cyclodextrin are made into 3% aqueous solution, are pressed in the machine spray gun; The 29g VREF is put in the drying tower, and starting device is adjusted 65 ℃ of EATs, and open simultaneously spray gun and be sprayed onto on the VREF in the mode of high-pressure fog, approximately 1 hour, treat its substantially dry, moisture is less than 7%;
2) 15g cyclodextrin, 10g gelatin are made into 4% coating buffer, are pressed in the machine spray gun; Starting device is adjusted 65 ℃ of EATs, and the mode with high-pressure fog is sprayed onto above-mentioned steps 1 simultaneously) on the product that obtains, approximately 2 hours, until drying materials;
3) with the 0.7g calcium pantothenate, 0.1g folic acid, 0.12g Cobastab 1, the 0.1g Cobastab 12, the 0.2g biotin, the bentonite of 70g is put into the mixer premix, incorporation time 5~10 minutes;
4) with step 2), step 3) and vitamin A 1.7g, vitamin D 0.4g, vitamin E 2.9g, vitamin K 0.3g, Cobastab 20.57g, Cobastab 60.12g, niacinamide 1.4g, bacillus subtilis 110g, FOS 10g, oligomeric maltose 57g, sucrose 15g, tert-butyl-5-methyl phenol 6g, uncle's propyl group-tetrahydroxy anisole 6g, silica 3.17g, mineral white oil 4.5g, pine needle meal 190g, bentonite 491.42g fully mixed 15 minutes in mixer, obtained the 1000g finished product.
Embodiment 2
The probiotics of patent of the present invention comprises following component: vitamin A 1.9g, vitamin D 0.4g, vitamin E 3.1g, vitamin K 0.2g, Cobastab 10.2g, Cobastab 20.5g, Cobastab 60.14g, Cobastab 120.1g, niacinamide 1.5g, calcium pantothenate 0.9g, folic acid 0.1g, biotin 0.3g, VREF 24g, bacillus subtilis 117g, oligosaccharides 89g, pine needle meal 180g, bentonite 563.09g, tert-butyl-5-methyl phenol 6g, uncle's propyl group-tetrahydroxy anisole 6g, silica 3.17g, mineral white oil 4.3g, cyclodextrin 40g, cellulose 15g, converted starch 10g, gelatin 10g; Oligosaccharides comprises FOS 17g, oligomeric maltose 57g, sucrose 15g.
Follow these steps to preparation:
1) 15g cellulose, 10g converted starch, 25g cyclodextrin are made into 3% aqueous solution, are pressed in the machine spray gun; The 41g VREF is put in the drying tower, and starting device is adjusted 65 ℃ of EATs, and open simultaneously spray gun and be sprayed onto on the VREF in the mode of high-pressure fog, approximately 1 hour, treat its substantially dry, moisture is less than 7%.
2) 15g cyclodextrin, 10g gelatin are made into 4% coating buffer, are pressed in the machine spray gun; Starting device is adjusted 65 ℃ of EATs, and the mode with high-pressure fog is sprayed onto above-mentioned steps 1 simultaneously) on the product that obtains, approximately 2 hours, until drying materials.
3) with the 0.9g calcium pantothenate, 0.1g folic acid, 0.2g Cobastab 1, the 0.1g Cobastab 12, the 0.3g biotin, the bentonite of 75g is put into the mixer premix, incorporation time 5~10 minutes;
4) with step 2), step 3) and vitamin A 1.9g, vitamin D 0.4g, vitamin E 3.1g, vitamin K 0.2g, Cobastab 20.5g, Cobastab 60.14g, niacinamide 1.5g, bacillus subtilis 117g, FOS 17g, oligomeric maltose 57g, sucrose 15g, tert-butyl-5-methyl phenol 6g, uncle's propyl group-tetrahydroxy anisole 6g, silica 3.17g, mineral white oil 4.3g, pine needle meal 180g, bentonite 488.09g fully mixed 15 minutes in mixer, obtained the 1000g finished product.
Test example 1
Materials and methods
1.1 experimental design
5 processed group are established in the design of this test employing single-factor contrast test, namely test 1 group, test 2 groups, test 3 groups, test 4 groups, test 5 groups.Test 1 group, 2 groups, 3 groups, 4 groups, 5 groups at respectively interpolation 0g per ton, 250g, 500g, 700g, 1000g embodiments of the invention 1 on the basis of chick basal diet.
1.2 experimental animal
Select 400 Ai Wei mattress meat chicks, be divided at random 5 groups, establish 4 repetitions for every group, each repeats 20.
1.3 test daily ration
The common negotiation of Beijing Fuwish Biotechnology Co., Ltd and plant determined
1.4 feeding and management
Experimental animal is raised in cages, and the test cage is 3 ply formula breeding cages, free choice feeding and drinking-water, temperature, humidity and illumination in the control house.
1.5 testing index and method
Average daily gain: during on-test, chick is weighed; Weigh weekly once afterwards, weighed in 12 hours on an empty stomach, and do detail record, calculate the test average daily gain.
Average daily ingestion amount: add up weekly the test chicken feed consumption rate 1 time, clout reclaims, and calculates average daily ingestion amount.
Material anharmonic ratio: average daily ingestion amount/average daily gain.
Death rate: extremely wash in a pan chicken number/overall test chicken number.
1.6 data are processed
Data statistics adopts the ANOVA program among the SAS8.2 to carry out statistical analysis, uses Duncan ' s method to carry out multiple ratio.The result represents with " average ± standard deviation ".
2 results and analysis
Table 1 is on the impact of growth of meat chicken performance and death rate
Can find out from table 1 and Fig. 1, along with the increase of fowl of the present invention with probiotics use amount on broiler chicks, average daily gain, average daily ingestion amount also increase thereupon, and the material anharmonic ratio reduces along with the increase of use amount.Average daily gain is tested 2 groups than 1 group high by 4.97% of test, tests 3 groups than 1 group high by 9.39% of test, tests 4 groups than 1 group high by 10.38% of test, tests 5 groups than 1 group high by 11.15% of test.Daily ingestion amount is tested 2 groups than 1 group high by 2.97% of test, tests 3 groups than 1 group high by 6.05% of test, tests 4 groups than 1 group high by 7.01% of test, tests 5 groups than 1 group high by 8.19% of test.The material anharmonic ratio is tested 2 groups than 1 group of reduction by 1.91% of test, tests 3 groups than 1 group high by 3.05% of test, tests 4 groups than 1 group high by 3.05% of test, tests 1 group of 5 groups of ratio test and reduces by 3.44%.
Lactic acid bacteria in the ight soil/colibacillary ratio is a key index weighing the intestinal health situation.Bacillus in the probiotics of the present invention is by consuming the oxygen in the enteron aisle, reduce the redox potential in the enteron aisle, creation is conducive to the environment of anaerobic bacteria growth, foster the dominant microflora in the enteron aisle, the quantity that suppresses the harmful bacterias such as Escherichia coli, flora structure in the regulating intestinal canal makes it the state that reaches healthy.And VREF is exactly one of lactic acid bacteria of dominant microflora in the enteron aisle originally, extra quantity of adding can make simple carbohydrate produce a large amount of lactic acid, thereby the pH in the reduction enteron aisle is suppressed the neutral pernicious bacteria growth to alkaline environment of some preferences.And VREF is as one of dominant microflora in the enteron aisle, and amount reproduction is conducive to the raising of growing of animal and production performance.
Test example 2
Materials and methods
1.1 experimental design
The design of single-factor contrast test is adopted in this test, establishes 1 control group and 1 test group, and the control group fed basal diet adds embodiments of the invention 2 by 500g/t in the basal diet that test group is fed.
1.2 experimental animal
720 316 ages in days, the extra large blue brown shell layer that body weight is close.Be divided at random control group and test group, each processes minute 6 repetitions, and each repeats 60.
1.3 test daily ration
The common negotiation of Beijing Fuwish Biotechnology Co., Ltd and plant determined.
1.4 feeding and management
The test chicken feeding manner is that staged is raised in cages, 3 chickens of every cage.Fed by the special messenger, feeding and management is identical, all adopts hand fed, free choice feeding and drinking-water, and artificial insemination, every day, light application time was 16 hours, intensity of illumination is 10 Luxs.The phase of raising is 3 days in advance, two groups of basal diets of all feeding.The phase of raising in advance, the indices difference of test group and control group observation was all not remarkable when finishing.
1.5 testing index and method
33 days experimental periods, after on-test, record feed consumption rate, egg number, hatchable egg rate, extremely naughty chicken number every day, the state of mind of observing chicken, drinking-water situation, fecal nature.
Since the 15th day of experimental period, per 3 days was a collection of, selected the mensuration that qualified kind egg carries out rate of fertilization, incubation rate, strong young rate for every group continuous 6 batches.
30 eggs of every group of random extraction are surveyed egg size, shell thickness, hangh unit when experimental period finishes.
Reproductive performance:
Hatchable egg rate (%)=qualified kind the egg number/total * 100% lays eggs
Rate of fertilization (%)=fertile egg number/enter incubation to count * 100%
Incubation rate (%)=chickling-surviving number/enter incubation to count * 100%
Strong young rate (%)=strong young rate/chickling-surviving number * 100%
Death rate (%)=is extremely washed in a pan chicken number/raising chicken and is counted * 100%
The Egg Quality index:
Shell thickness (mm) is measured respectively blunt end, middle part, the sharp end of egg and is averaged after getting shell thickness.
Hangh unit=100Log (H-1.7W 0.37+ 7.57) H-is dense albumen height, take millimeter as unit; W-is egg size, in grams.
The minor axis of the major diameter/egg of egg shape index=egg
1.6 data are processed
Data statistics adopts the ANOVA program among the SAS8.2 to carry out statistical analysis, uses Duncan ' s method to carry out multiple ratio.The result represents with " average ± standard deviation ".
2 results and analysis
2.1 on kind of the impact of chicken reproductive performance of laying eggs
Table 2 is on kind of the impact of chicken reproductive performance of laying eggs
Can find out from table 2 and Fig. 2, along with the increase of fowl of the present invention with probiotics use amount on kind of the chicken of laying eggs, hatchable egg rate, rate of fertilization, incubation rate, strong young rate also increase thereupon, and death rate reduces along with the increase of use amount.The test group hatchable egg rate exceeds 6.03% than control group, and the test group rate of fertilization exceeds 1.92% than control group, and the test group incubation rate exceeds 6.32% than control group, and the strong young rate of test group exceeds 4.68% than control group.Death rate, test group has reduced by 75.3% than control group.
2.2 the impact on Egg Quality
Table 3 is on the impact of Egg Quality
Project Control group Test group
Shell thickness, mm 0.332±0.026 0.355±0.032
Hangh unit 80.41±5.26 85.69±5.12
The egg shape index 1.301±2.44 1.308±3.17
As can be seen from Table 3, add in the diet that shell thickness and hangh unit all increase behind the present invention, good shell thickness is generally about 0.33~0.35mm, shell thickness is when 0.25mm is following, can produce about 85% check, shell thickness is larger, and the fresh-keeping degree of egg is just higher, and the nutritive peculiarity holding time of egg is longer for a long time.Hangh unit is the freshness of weighing protein quality and egg, and the Hardgrove index of fresh-laid egg is more than 80, and dense albumen is higher, and egg is just fresher, and hangh unit is just larger, and hangh unit and incubation rate are closely related.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
4 conclusions
The present invention is as a kind of novel fowl probiotics, being used for bird raises, the production performances such as daily ingestion amount that can the Effective Raise poultry, daily gain, feed efficiency, can improve simultaneously the reproductive performances such as hatchable egg rate, rate of fertilization, incubation rate, strong young rate of kind of fowl, increase economic efficiency.

Claims (6)

1. a novel fowl probiotics is characterized in that, comprises by weight following active ingredient: vitamin A 0.2-4.5 part, vitamin D 0.2-0.9 part, vitamin E 2-8 part, vitamin K 0.1-1.2 part, Cobastab 10.05-0.9 part, Cobastab 20.2-1.2 part, Cobastab 60.01-0.5 part, Cobastab 120.03-0.6 part, niacinamide 1-6 part, calcium pantothenate 0.3-5 part, folic acid 0.02-0.9 part, biotin 0.05-1.4 part, vitamin C 0.01-2 part, VREF 1.2-38 part, bacillus subtilis 16-155 part, oligosaccharides 70-260 part, pine needle meal 150-400 part, bentonite 350-600 part.
2. fowl probiotics according to claim 1 is characterized in that, comprises by weight following active ingredient: vitamin A 0.8-3.5 part, vitamin D 0.2-0.8 part, vitamin E 2-8 part, vitamin K 0.2-1.2 part, Cobastab 10.1-0.9 part, Cobastab 20.2-0.8 part, Cobastab 60.1-0.7 part, Cobastab 120.08-0.5 part, niacinamide 2-5 part, calcium pantothenate 0.8-3.5 part, folic acid 0.02-0.4 part, biotin 0.2-1.2 part, vitamin C 0.02-2 part, VREF 1.3-32 part, bacillus subtilis 27-155 part, oligosaccharides 75-210 part, pine needle meal 150-400 part, bentonite 400-600 part.
3. fowl probiotics according to claim 1 is characterized in that, also comprises the additive of following weight parts: cyclodextrin 15-79 part, cellulose 9-28 part, converted starch 5-31 part, gelatin 2-23 part, anti-current powder 1-6 part, antistatic additive 1-6 part, antioxidant 0.5-20 part.
4. fowl probiotics according to claim 1 and 2 is characterized in that, described oligosaccharides is the mixture of FOS, oligomeric maltose and sucrose, and the weight ratio of three kinds of components is 5-80: 5-80: 5-60.
5. fowl probiotics according to claim 3 is characterized in that, described antioxidant is tert-butyl-5-methyl phenol and uncle's propyl group-tetrahydroxy anisole mixture, and weight ratio is 1: 1-3.
6. one kind prepares each described fowl of claim 1-5 with the method for probiotics, it is characterized in that, comprises the steps:
The cellulose, converted starch, cyclodextrin that 1) will account for probiotics quality 2-10% are by 100-500 part, 100-300 part, and the ratio of 200-800 part is made into the 3-10% aqueous solution; Mentioned solution is pressed in the machine spray gun; Starting device is adjusted 60-100 ℃ of EAT, and the mode with high-pressure fog is sprayed onto on the VREF simultaneously, and approximately 1-2 hour, treat its drying, moisture is less than 7%;
The cyclodextrin, gelatin that 2) will account for probiotics quality 1-3% are by 200-800 part, and the ratio of 100-900 part is made into 2~20% coating buffer; Mentioned solution is pressed in the machine spray gun; Starting device is adjusted 60~100 ℃ of EATs, and the mode with high-pressure fog is sprayed onto on above-mentioned steps 1 encrusting substance simultaneously, and approximately 2-3 hour, until drying materials;
3) by said ratio, with said vitamin monomer Folic Acid, calcium pantothenate, Cobastab 1, Cobastab 12, biotin, the bentonite of 1/10-1/5 amount is put into the mixer premix, incorporation time 5~10 minutes;
4) with step 2), step 3) product and the remaining ingredient mixer in fully mixed 10~15 minutes, and get final product.
CN2012103422824A 2012-09-17 2012-09-17 Novel poultry micro-ecological preparation and preparation method thereof Pending CN102885202A (en)

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CN103478435A (en) * 2013-10-18 2014-01-01 山东新希望六和集团有限公司 Chinese medicinal composition and feed for improving fertility rate of male breeding poultries and preparation method of composition and feed
CN103652369A (en) * 2013-12-04 2014-03-26 北京福乐维生物科技股份有限公司 General-purpose livestock nano nutritional agent having anti-stress function and preparation method of the nutritional agent
CN104012753A (en) * 2013-12-24 2014-09-03 北京伟嘉人生物技术有限公司 Active microorganism functional feed additive for livestock and preparation method and application thereof
CN104099280B (en) * 2014-07-17 2016-09-07 湖北华大瑞尔科技有限公司 One strain alleviate livestock and poultry stress bacillus subtilis seed selection and application
CN104099279A (en) * 2014-07-17 2014-10-15 湖北华大瑞尔科技有限公司 Compound micro-ecological preparation for relieving stress of livestock and poultry and application
CN104099280A (en) * 2014-07-17 2014-10-15 湖北华大瑞尔科技有限公司 Selective breeding and application of bacillus subtilis for alleviating stress of livestock and poultry
CN104099279B (en) * 2014-07-17 2016-09-07 湖北华大瑞尔科技有限公司 A kind of alleviate livestock and poultry stress compound micro-ecological preparation and application
CN105166573A (en) * 2015-10-15 2015-12-23 格特生物制药(天津)有限公司 Vitamin compound premix for laying hens
CN107156436A (en) * 2017-06-13 2017-09-15 徐州德诚饲料有限责任公司 A kind of family goose mixed feed and preparation method thereof
CN107629981A (en) * 2017-10-13 2018-01-26 安徽农业大学 A kind of compound probiotic agent for improving chick function of intestinal canal and preparation method thereof
CN107629981B (en) * 2017-10-13 2019-09-27 安徽农业大学 A kind of compound probiotic agent and preparation method thereof improving chick function of intestinal canal
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CN107828680B (en) * 2017-11-01 2021-03-12 天津博菲德科技有限公司 Composite microecological preparation and preparation method and application thereof

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