KR100839038B1 - A probiotic complex comprising organic metabolites and the preparing process thereof - Google Patents

Abstract

The present invention relates to an organic metabolite-containing biocomposite and a method for manufacturing the same, and more particularly, to inoculate a strain mixture into an organic fermentation medium having a predetermined composition and conditions, and to produce the product as a feed additive which is commercialized through fermentation, drying and grinding. It relates to an antibacterial-containing biocomposite and a method for producing the same. Therefore, the probiotic complex according to the present invention is added to the feed as a probiotic in the animal, as well as the probiotics effect, increase the growth and feed distribution rate, and also promote the growth of plants, as well as strengthen the fibrous tissue and firm skin It is effective in plant fertilizer or nutrient cultivation of plants by protecting them from pests.

Probiotics, Probiotics, Organic Metabolites

Description

A probiotic complex comprising organic metabolites and the preparing process

Figure 1a and 1b is a diagram recording the temperature and humidity in the coop during the experimental period in Experimental Example 1 supplied with the biocomposite according to the present invention.

Figure 2 is a diagram measuring the mortality until shipment in Experimental Example 2 supplied with the biocomposite according to the present invention.

Figure 3 is a comparative photograph showing the effect on the growth of plants in Experimental Example 3, which is supplied with a biocomposite according to the present invention.

The present invention relates to an organic metabolite-containing biocomposite and a method for manufacturing the same, and more particularly, to inoculate a strain mixture into an organic fermentation medium having a predetermined composition and conditions, and to produce the product as a feed additive which is commercialized through fermentation, drying and grinding. It relates to an antibacterial-containing biocomposite and a method for producing the same.

Conventionally, antibiotics have been used for disease treatment, prevention and growth effects of livestock or poultry. However, the current use of antibiotics has caused antibiotic resistance in pathogens or livestock products with antibiotics remaining. The problem that becomes high has arisen.

In recent years, probiotics have been developed as a new alternative to antibiotics and are being distributed to many farms. Here, probiotics means 'living microorganisms affecting the intestinal microbial balance' [Parker R.B., An Nutr Health, 29; 4-8, 1974], these probiotics allow the intestinal flora to be well developed, resulting in the maturation of beneficial microbiome in the gut with the concept of competitive exclusion, the 'Nurmi Concept', which subsequently prevents the proximity of pathogenic microorganisms such as Salmonella. Nurmi E. et al., Nature, 241; 210, 1973], which is useful for improving livestock growth and feed requirements by producing vitamins, enzymes and nutrients in the intestine, and by dissolving and supplying undigested nutrients to the host, and adding to plant fertilizers or nutrient solutions. It is known that it is useful for plant cultivation by promoting the growth of municipal plants and inhibiting the aging of roots. In addition, the effect of using probiotics has already been demonstrated in many cases. See, Republic of Korea Patent Publication No. 2002-0043078; Republic of Korea Patent Publication No. 2002-0011042].

By the way, the production method of such probiotics was common to ferment the microorganisms mainly in the liquid phase and mixed with the excipients, but this method is difficult to maintain the viable count for a long time, and simply by mixing the strain with the excipients, enzymes and fermentation products are cultured There was a problem that it is impossible to produce a probiotic in the form as it is. In addition, in order to improve the above problems, the first liquid fermentation method followed by secondary fermentation in a Coogee medium or a method of varying the fermentation conditions of several stages after inoculation of the strain into the fermentation broth has been developed. There was a complicated process by using the fermentation method [Korean Patent Publication No. 2002-0014962; Republic of Korea Patent Publication No. 2002-0011042].

Therefore, in the case of the probiotic, a sufficient amount of useful strains can be obtained in the fermentation product, and there is still a need for a simpler manufacturing method and a product containing the same, which can produce the functional fermentation product as it is.

Thus, the inventors of the present invention, as a result of diligent efforts to solve the above problems, as a medium of a certain amount of fruit liquid, corn starch and sugar cane inoculated at the same time to inoculate Lactobacillus and Saccharomyces known as useful strains, By cultivating under conditions, it is possible to obtain a sufficient amount of useful strains capable of sufficiently exhibiting the bioactive function of animals and plants, as well as to produce the functional fermented product itself as it is, so that it can be produced in an easier way.

After all, it is an object of the present invention to provide a biocomposite containing an organic metabolite that helps the physiological activity of animals and plants and a method for producing the same.

In order to achieve the above object, the present invention provides a method for producing a biocomposite, (i) 15 to 20% by weight of fruit liquid consisting of apple, orange, carrot and aloe, 78.5 to 84.5% by weight of corn starch; And Fermentation medium manufacturing step comprising the step of combining the sugar cane 0.5 to 1.5% by weight; (Ii) inoculating the strain to inoculate Lactobacillus sp. And Saccharomyces sp. (Iii) fermenting for 70 to 80 hours at a temperature of 35 to 45 ° C. and a humidity of 95 ± 5% after inoculation of the strain; And (iii) drying the fermented product at 70 to 75 ° C. for 60 to 72 hours to form a moisture content of less than 1 to 10%, wherein the fruit liquid is apple and orange based on 100 parts by weight of carrot. 3.34 parts by weight, and a method for producing an organic metabolite-containing biocomposite containing about 20 parts by weight of aloe.

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In another aspect, the present invention provides an organic metabolite-containing biocomposite prepared by the above production method.

In addition, the present invention is the combination agent Lactobacillus ( Lactobacillus sp . ) 1.0X10 ⁷ To 1.0 × ^{10 8} cfu / g, Saccharomyces sp . ) Provides an organic metabolite-containing biocomposite containing 1.0X10 ⁷ to 1.0X10 ⁸ cfu / g.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. First of all, it should be noted that in the drawings, the same components or parts denote the same reference numerals as much as possible. In describing the present invention, detailed descriptions of related well-known functions or configurations are omitted in order not to obscure the subject matter of the present invention.

As used herein, the terms "about", "substantially", and the like, are used at, or in close proximity to, numerical values when manufacturing and material tolerances inherent in the meanings indicated are intended to aid the understanding of the invention. Accurate or absolute figures are used to assist in the prevention of unfair use by unscrupulous infringers.

First, the present invention relates to a method for producing an organic metabolite-containing biocomposite,

(Iii) 15-20% by weight of one or more fruit liquors selected from the group consisting of apples, oranges, carrots and aloes, 78.5-84.5% by weight of corn starch; And fermentation broth manufacturing step of mixing the sugar cane 0.5 to 1.5% by weight and maintaining the pH of the medium at pH 2.5 to 3.5;

(Ii) Lactobacillus in the fermentation medium sp . ) And a strain inoculation step of inoculating Saccharomyces sp .

(Iii) fermenting for 70 to 80 hours at a temperature of 35 to 45 ° C. or below and a humidity of 95 ± 5% after inoculation of the strain; And,

(Iii) drying the fermented product at 70 to 75 ° C. or less for 60 to 72 hours to form a moisture content of less than 1 to 10%.

In the (i) fermentation broth preparation step of the present invention, the fermentation broth includes fruit liquid, corn starch and sugar cane. Here, the fruit liquid is again selected from the group consisting of apples, oranges, carrots and aloe, of which apples or oranges are added to properly maintain the acidity in the fermentation medium at pH 2.5 to 3.5 and use it as a nutritional source, fermentation medium 15 to 20% by weight of the total content. More preferably, all of the apples, oranges, carrots, and aloe may be included. At this time, about 3.34 parts by weight of apples and oranges and about 20 parts by weight of aloe may be included with respect to 100 parts by weight of carrots. The apples and oranges serve to maintain acidity at an appropriate level in the medium, and aloe is particularly effective in increasing the rooting effect of plants. And, corn starch is included as a nutritional source, preferably 78.5 to 84.5% by weight of the total content of fermentation broth. Finally, the sugar cane is included as a small amount as a nutritional source, preferably containing 0.5 to 1.5% by weight of the total content of fermentation broth.

Then, (ii) the strain inoculation step is inoculated with Lactobacillus ( Lactobacillus sp . ) And Saccharomyces sp . At this time, Lactobacillus is a lactic acid bacterium that breaks down high-molecular organic materials (fats or proteins) to produce useful substances, produces organic acids such as lactic acid and acetic acid, has bile and acid resistance, and produces digestive enzymes to feed the help digestion and absorption as yuyonggyun that the metabolic product is absorbed by the cell wall components stimulate the specific immune mechanisms of the animal, Lactobacillus ash FIG filler's (L. acidophilus), Lactobacillus Cathay (L. casei), Lactobacillus sports Rosen ( L. sporogenes ), L. delbruekii , L. plantarum , and the like, in particular L. acidophilus is most preferably used. Do. Saccharomyces also supports the growth of lactic acid bacteria as active yeast, induces starch decomposition to produce monosaccharides such as glucose, and when the cell wall is decomposed, the oligosaccharides found in yeast cells come out as soluble polysaccharides. Agglomerates and excretes out of the intestines of animals. In addition, it contains abundant amino acids, various vitamins, minerals and unknown growth factors, and produces various digestive enzymes to promote digestion and absorption of feed nutrients, contributing to the normal growth and productivity of animals, and also acting as alcohol and glutamic acid. As a useful bacterium that has a function of enhancing palatability, increasing protein utilization, lowering fecal nitrogen content and reducing ammonia generation, Saccharomyces cerevisiae is most representative.

(Iii) Third, the fermentation stage is most important for the optimum fermentation conditions for the growth of effective fermentation strains. That is, the temperature after the strain inoculation is more preferably 35 to 45 ℃, the humidity is more preferably 95 ± 5%. In addition, the fermentation time is most preferably fermented for 70 to 80 hours under the temperature and humidity conditions. Because, too low compared to the temperature, humidity, time standard is not preferable because the fermentation does not proceed, even if too high, the microbial survival rate is not preferable. In addition, the configuration and function of the fermenter does not constitute the present invention, and since it uses a known one, detailed description thereof will be omitted.

Finally, (iii) the drying step is to dry the fermentation product for 70 to 75 ℃, 60 to 72 hours, if the temperature is too high, the cultured strain is killed and the survival rate is lowered, so the temperature condition is reduced It is important to adjust. In addition, the drying time can also be adjusted according to the moisture content of the fermentation product, usually 60 to 72 hours is most preferred, the final moisture content should be less than 1 to 10%. This is because if the moisture content is too high, it becomes corrupt and cannot be commercialized. In addition, additional secondary fermentation may be further performed in the drying step.

Then, the dried fermented product is subjected to pulverization and packaging step according to a conventional method to produce a product. As a result, the biocomposite prepared according to the production method of the present invention not only contained useful strains in an amount capable of exhibiting sufficient functionality for livestock, poultry, or plant growth, but also contained live metabolites as a source of nutrition. The composite product can be manufactured.

In addition, the present invention is prepared by the production method as described above, the useful strains, as well as the organic metabolite-containing biocomposite containing a sufficient source of nutrition. That is, the present invention includes a biocomposite containing an organic metabolite sufficiently matured by the useful strain, prepared by the above method. At this time, the biocomposite according to the present invention in order to be able to exert a functional in the host Lactobacillus ( Lactobacillus sp. ) Is 1.0X10 ⁷ to 1.0X10 ⁸ cfu / g, Saccharomyces sp. 1.0X10 ⁷ to 1.0X10 ⁸ cfu / g need to be included, they are used in addition to the feed in the content of 0.0005 to 0.0015% (w / w) of the total content of the feed. This is because if the amount of the biocomposite added is too small or too large, it is ineffective or inefficient.

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The organic metabolite-containing biocomposite according to the present invention prepared as described above is added to the feed as a probiotic in the animal, as well as to increase the probiotics (Probiotics) effect, growth and feed rate of the plant, as well as to promote plant growth It is effective in plant fertilizers or nutrient cultivation of plants by expanding root roots, strengthening fibrous tissues, and strengthening the bark to protect against pests. It also has the effects of deodorization, water purification and acidification.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but the present invention is not limited thereto.

Example 1 Preparation of Probiotic Complex

(Iii) 19% by weight of a fruit liquid consisting of 0.5 parts by weight of apple, 0.5 parts by weight of orange, 15 parts by weight of carrot and 3 parts by weight of aloe; Corn starch 80% by weight; And 1% by weight of sugar cane to prepare a fermentation broth. (Ii) The fermentation medium was inoculated with Lactobacillus sp. And Saccharomyces sp . (Iii) After inoculation of the strain, the fermenter was kept at 45 ° C. and humidity at 95 ± 5% for 75 hours. Then, (iii) the fermentation product was dried at 75 ° C. for 70 hours and then ground to prepare a biocomposite. As a result, Lactobacillus sp. And Saccharomyces sp. Contained in the biocomposite were 1.8 × 10 ⁷ cfu / g and 4.2 × ^{10 7} cfu / g, respectively.

Experimental Example  One

1. Essentials

(1) Test subject

308 roosters were provided by Golden cockerel Pty / Ltd (Mt Cotton) for a total of 384 days, and all hatched chicks were vaccinated. The test subjects were housed in 48 chicken coops, each containing 8 feeds, powder feeds from 1 to 21 days, and kernel feeds from 21 days to the last 42 days.

(2) Test subject and maintenance state

While keeping a close eye on the health of the chickens, the poor or uneconomical chickens were excluded from the experiment, and the severely tortured chickens were killed by cervical dislocation and sent to the pathology department for necropsy. Was sent for autopsy. Below is a list of symptoms that have a "negative effect" or "extreme pain" in the experiment.

Chickens with reduced activity and environment

-Chicken with blurred eyes compared to other chickens

-Chickens with abnormal hair

-Slow-moving chicken

-Chickens that don't eat any water or water at all

-Chickens physically injured by mutual attack

-All chickens with diarrhea, broken beaks, dyspnea, lameness or unusual symptoms

  Dietary feed and drinking water were provided to all chickens in an unlimited number and at least twice a day were observed.

(3) Experimental environment

Experiments were conducted in 96 independent houses with reverse circulation air conditioning. Ventilation is connected to the air conditioning system to reduce the amount of chemical reaction emissions of ammonia and oxygen and to provide fresh air at all times inside the house.

In the first stage (1-21 days), they were reared in henhouses 66 cm long, 35 cm wide and 40 cm high, and in the middle and last stages (21-42 days) were (95 X 70 X 40 cm). Feed and drinking water were provided indefinitely during the 23 hours / time of use of the lighting system, and the room temperature gradually decreased from day 1 to day 42. Chickens that died or were found within the first 72 hours were replaced by healthy chickens, after which no further replacements were made.

(4) drinking water

Water supply was provided for the convenience of all barns and chickens. Each cage is designed with an easy-to-inject piped water supply, so you can easily drink at any time.

2. Experiment Planning

(1) principle

The experiment suggests the use of a minimum number of experiments in order to maximize the weight change, feed intake, and feed efficiency of the population. This is to find out more economically significant differences through each experiment with various convenient facilities. It is. The range of error of mean deviation in feed efficiency (feed ratio) is around 0.05. Here, 0.05 means 5% of the mean error, which means that you expect a slightly higher or lower value. Therefore, finding remarkable differences through the eight experimental populations used in this experiment is indispensable.

The experimental design, consisting of 48 stalls that were completely randomized, would apply six different experiments to eight test subjects. Six different feed mixtures shall be distributed to the house. Each barn consists of eight roosters.

Experimental Planning Planning Form: Eight Completely Randomly Evicted Experimental group , 6 prescriptions Experiment prescription type 24 Trial type Nutrient evaluation Experimental unit One barn with eight chickens Type of experiment prescribed for each experimental unit Use six different prescriptions Total number of experimental units 48 barns The number of individuals constituting one experimental unit 8 Key variables to observe Feed intake, weight gain, feed distribution

(2) Application to test subject

On the day of arrival of the chickens used for all experiments, after the individual weight measurement, the total weight of the initial individual chicken coop (P> 0.05) was not significantly different since it was housed in the chicken coop without specific classification. Chickens that are sick or uneconomical and do not meet the reference weight are excluded during the experiment. The weight per cage measured by the electronic balance is described in Table 2 below. Chickens that died or had problems during the first 72 hours were either removed or replaced, but no replacements were made for later killed or problematic chickens.

Feed NO Feed Processing Method Barn, Gender Feed 1 Chicken feed for corn-beans 8 roosters each at 8 stalls Feed 2 Add 2 kg Example 1 1 / tone to feed 1 8 roosters each at 8 stalls Feed 3 0.1kg avilamycin / tone added to 1 8 roosters each at 8 stalls Feed 4 Fodder for wheat 8 roosters each at 8 stalls Feed 5 Add 2 kg Example 1 1 / tonne to feed 4 8 roosters each at 8 stalls Feed 6 Add 0.1kg avilamycin / tonne to 4 8 roosters each at 8 stalls

avilomycin: A commercially available (Surmax) growth accelerator by animal health expert Elanco, widely used in the Australian livestock industry. In this experiment, we will use the term avilamycin, which collectively refers to the oligosaccharide antibiotic of the orthosomysin group. Avilomycin is a feed-enhancing additive for chickens to increase feed efficiency and increase weight percentage. All feeds contain commercially available enzymes and Coccidiostat. The experimental design was thoroughly randomly selected from 48 stalls, and eight specimens were selected for each stall, and a total of eight specimens were processed through six different diets.

3. Experimental Materials and Methods

(1) Feeding method

Powder feed was provided at the beginning of the experiment (1-21), and kernel feed was provided at the growth stage and at the end of the experiment (22-42).

(2) Material blend

Cereals, protein foods and additional ingredients were purchased from local and state suppliers in Australia. Each material was prepared after a chemical analysis of all the nitrogen phosphate before it was officially used in the experiment. The feed received is in the form of each dry kernel, which is sent to a manufacturing plant using a vertical mixer (up to 1 ton capacity). Each feed is approximately 20 kg removed from the vertical mixer and placed back into the Hobart mixer (capacity up to 40 kg). The appropriate amount of Example 1 is weighed together with vitamins, mineral chemical amino acids and enzymes and mixed together before entering the Hobart mixer (40 kg). After 5-7 minutes, the mixture consisting of Example 1 is placed in a 1 ton vertical mixer with the premixed feed and mixed slowly while maintaining careful handling of the feed (mixing while rotating). After 15 minutes the feed mass is Vertival holding bin. And are compressed into a kernel-shaped steam machine. Each feed is pressurized at a temperature of 65-70 degrees, converted into a kernel form, and then immediately transported to a freezer to cool down quickly. The cold kernel feed is then sent back to the grinder and then to a large plastic container through an agitator for proper sorting.

(3) Research observation

① Environment

 When controlling the temperature inside the house, management by computer system was made for more effective management and control.

  ② feed intake

 The intake of released feed was measured during every 1, 21 and 42 days of the experiment. The breeder recorded the difference between the amount of food released and the amount provided to the chickens in the house. Feed intake was measured for each group on days 21 and 42, and when they died, the amount returned immediately was recorded that day.

  ③ weight gain

 Weight gain was measured for changes from 1 to 21 days and from 21 to 42 days, and the weight of each group was recorded every 21 days and 42 days. The chickens in each barn were weighed and recorded in plastic plates on the 21st day, and on the 42nd, the chickens were weighed using a flat end connected to a portable electronic balance.

  ④ Health condition

 The condition of the chicken was observed twice a day, and the chickens with severe pain or signs of death were recorded separately. Observers do not exclude chickens with pain or leg problems (common in poultry experiments). Dead chickens were removed and the expected cause of death was noted.

  ⑤ Data Analysis

 All materials were recorded by hand and kept in the form of spreadsheets in computer programs. Interim reporting of data for broiler products (beef chickens) took place on 21 and 42 days. Data are analyzed using six feeds based on corn / soybeans and wheat / soybeans. In analyzing the changes in the experiments, six dietary treatments were applied to eight experimental specimens consisting of eight chickens randomly removed from 48 cages.

4. Results

(1) environment

Figure 1 records the temperature and humidity in the coop during the experiment. Temperature management in the house is programmed. 33.0 degrees and 26.1 degrees represent the highest and lowest temperatures, respectively, and the average temperature is 26.1 degrees. The highest and lowest humidity is 86.6 percent and 46.2 percent, respectively, and the average humidity is 70 percent.

(2) Statistical analysis of parametric research results

The evaluation of experiments over 21 and 42 days investigated the chicken's feed intake (BFI) weight gain (LWG) and feed distribution (FCR).

(3) initial weight (g / cage)

Prescription Diet Prescription One Corn / bean 39.7 2 Add 2 kg of Example 1 to feed 1 40.4 3 Add 0.1 kg of Antibiotic to Feed 1 39.8 4 Wheat / Bean 39.8 5 Add 2 kg of Example 1 to feed 4 40.3 6 Add 0.1 kg of Antibiotic to Feed 5 40.1

LSD (P = 0.05)

Initial weights measured on the first day showed little difference during the defined diet (P> 0.05).

(4) feed intake

Feed intake was measured as follows on the 21st and 42nd days, and the 22nd to 42nd days, respectively.

BFI21: Measure the amount of feed consumed only for chickens that survived mortality adjustment on day 21

BFI42: Measured feed intake only for chickens that survived mortality adjustment on day 42

BFI22-42: Determination of feed consumed only for surviving chickens after adjusting for mortality for 22 to 42 days

Feed intake for 21-42 days and 22-42 days from the experimental date Prescription feed BFI 21 BFI 21-42 BFI 42 One Corn / bean 1030 3450 4480 2 Add 2 kg of Example 1 to feed 1 1046 3374 4420 3 Add 0.1 kg of Antibiotic to Feed 1 1048 3434 4482 4 Wheat / Bean 1126 3732 4859 5 Add 2 kg of Example 1 to feed 4 1108 3728 4836 6 Add 0.1 kg of Antibiotic to Feed 5 1138 3674 4812 F probability ** <0.001 <0.001 <0.001 LSD (P = 0.05) 41.38 130.3 154.8 Ross 308 Ross 308 Manual 1069 3189 4258

On day 42, wheat feed intake of all chickens (375 g / sugar) was noticeably higher compared to corn feed (P <0.05). Accumulated during the second period (21-42 days). However, during the feeding of corn / soybeans and wheat / soybeans, there was little difference between the two trial periods. Through this experiment, the feed intake 1083 / g (mean value on the 21st day) showed a significant difference compared to the expected feed intake of 1069 / g of the rooster 308 experiment.

(5) weight gain (LWG)

On the 21st and 42nd day, the weight gain rate of the chicken was measured as follows.

LWG0-21: Weight gain (g / bird) d0> d21, (= BW21-BW0), i.e. the change is measured only for surviving chickens

LWG0-42: Weight change (g / bird) d0> d42, (= BW42-BW0), i.e. change in live chickens only

LWG22-42: Weight gain rate (g / bird) d0> d42, (= BW42-BW21), i.e. the weight change is measured only for surviving chickens

Body weight gain as measured on 21-42-42 Prescription feed LWG 21 LWG 21-42 LWG 42 One Corn / bean 808 2098 2907b 2 Add 2 kg of Example 1 to feed 1 819 2071 2889b 3 Add 0.1 kg of Antibiotic to Feed 1 839 2101 2940b 4 Wheat / Bean 884 2164 3049a 5 Add 2 kg of Example 1 to feed 4 866 2193 3060a 6 Add 0.1 kg of Antibiotic to Feed 5 874 2187 3060a F probability ** <0.001 <0.001 <0.001 LSD (P = 0.05) 33.16 80.3 99.4 Ross 308 Ross 308 Manual 778 1654 2432

On the 42nd day, the weight gain of wheat-fed chickens was significantly higher than that of corn-fed chickens. This difference in weight gain was cumulative over the first period (0-21 days) and the second (21-42 days). However, during the feeding of corn / soybeans and wheat / soybeans, there was little difference between the two trial periods.

In this experiment, average weight gain (LWG) after feeding on corn and wheat was 822 and 875 g / bird (day 21), respectively, slightly above the 778 G / bird suggested by the Ross 308 Manual. Although the level was 42 days, the measurement result (mean 2984) was remarkably high compared to the value 2432 suggested in Ross 308 Manual. Eventually, this huge difference in weight gain recorded 436 g / bird and 527 g / bird, respectively, in corn wheat during the 21-42 day trial.

(6) feed rate

Feed distribution rates were measured by PFI / PW at 21, 42 and 22-42 days (see Table 4).

Pen feed intake (PFI): Includes feed intake and all intakes of surviving chickens before death or exclusion from the barn

PW (Pen weight): Total Pen live-weight includes the weight of all dead or excluded chickens.

Feed Distribution Rate on Days 21-42 Prescription feed FCR 0-21 FCR 21-42 FCR 0-42 One Corn / bean 1,280 1,657 1,549 2 Add 2 kg of Example 1 to feed 1 1,729 1,630 1,530 3 Add 0.1 kg of Antibiotic to Feed 1 1,249 1,651 1,533 4 Wheat / Bean 1,276 1,713 1,596 5 Add 2 kg of Example 1 to feed 4 1,284 1,713 1,587 6 Add 0.1 kg of Antibiotic to Feed 5 1,303 1,701 1,580 F probability ** <0.005 <0.001 <0.001 LSD (P = 0.05) 0.02524 0.04237 0.03093 Ross 308 Ross 308 Manual 1,304 1,928 1,721

The difference in feed distribution (FCR) on the 42nd day of the experiment showed that the feed distribution by corn feeding (0.051) was much better than that when feeding wheat (P <0.05). Was cumulative during the second (21-42 days) following the first (0-21 days). When corn and wheat feeds were supplied, no significant differences were found during the 0-21 and 21-42 days of the experiment. In these experiments, the average FCR 1.269 and 1.288 for corn and wheat, respectively, were slightly better than the 1,304 expectations of the Ross 308 manual, but on day 42 the 1.563 FCR was significantly different from the 1.721 FCR of the Ross 308 manual. Recorded. In the end, the vast differences in FCR seen in this experiment were obtained for chickens grown with corn and wheat during the 21-42 days of the experiment, which also correlated with feed intake (BFI) and weight gain (LWG), which also showed excellent results. Can be.

Chicken Survival During Experiment Start Date (0-21) and Growth / End Date (21-42) Prescription feed NA 0-21 NA 21-42 Average One Corn / bean 7.62 7.25 7.4 2 Add 2 kg of Example 1 to feed 1 8.00 8.00 8.0 3 Add 0.1 kg of Antibiotic to Feed 1 7.62 7.38 7.5 4 Wheat / Bean 7.62 7.38 7.5 5 Add 2 kg of Example 1 to feed 4 7.38 7.0 7.2 6 Add 0.1 kg of Antibiotic to Feed 5 7.88 7.00 7.4 F probability ** 0.499 0.286 NS

5. conclusion

As all the above experimental data indicate, the efficacy and effect of Example 1 shows excellent results that were not found in the general feed. In general, chickens fed with wheat (P <0.05) were significantly better in feed intake (BFI) and weight gain (LWG) than chickens fed with corn (P <0.05). As a result obtained through this experiment, it is proposed to apply to the feed according to Example 1 in adjusting the ingredients of the feed did not show any effective response to poultry farming. This experiment was conducted under strict blocking of the house's environment, which could be affected by some bacteria. However, in a semi-commercial breeding environment (floor condition), chickens showed good poultry testing in all diets, although they were more likely to be exposed to bacteria.

Experimental Example  2

1. Test place: PKD broiler farm in Yangpyeong, Gyeonggi-do

2. Duration: About 35 days / Vaccine and antibiotics were administered according to the farm program

3. Test Purpose: Example 1 Test for reducing mortality by strengthening intestinal immune cells after feeding

4. Test Zone Assignment

Payroll Harvest Remarks Group 1 11,000 Example 1 Group 2 12,200 Lactic acid bacteria Group 3 12,200 Other company live bacteria + herbal medicine 4th group 9,500 5 groups 9,500

5. Test Result: Measurement of mortality until shipment

The mortality rate of the first-rate group fed Example 1 according to the present invention was significantly reduced (see Fig. 2).

Experimental Example  3: effect on plant growth

The biocomposite prepared in Example 1 was mixed with general soil to investigate the effect on the growth of Chinese cabbage. That is, the biocomposite was treated by 1 kg / 300m ² in the soil surrounding the cabbage seed, and the growth results of the same period of hair growth is shown in FIG. As a result, in the group treated with the biocomposite of the present invention, it was possible to harvest high-quality cabbage without growth of pests and even growth. (Sprinkle on top of soil)

Experimental Example  4: effect on plant growth ( Rooting effect )

The effect of the biocomposite prepared in Example 1 was mixed with general soil to examine the rooting of grass. (Victoria golf course, Yeoju, Gyeonggi-do) The biocomposite was treated by 1Kg / 300m ² , and the growth results obtained during the same period were collected with a hole cutter (grass cutter) to compare the rooting difference of grass roots. It can be seen that the roots of the grass treated in the example according to the present invention have excellent development status as shown in the following table.

Rooting effect comparison photograph division Before treatment After treatment Comparison picture

As described above, the present invention relates to an organic metabolite-containing biocomposite and a preparation method thereof. Therefore, the probiotic complex according to the present invention is added to the feed as a probiotic in the animal, as well as the probiotics effect, increase the growth and feed distribution rate, and also promote the growth of plants, as well as strengthen the fibrous tissue and firm skin It is effective in plant fertilizers or nutrient cultivation of plants by protecting them from pests.

The present invention described above is not limited to the above-described embodiment and the accompanying drawings, and various substitutions, modifications, and changes are possible within the scope without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

Claims (6)

In the method for producing a biocomposite, (Iii) 15 to 20% by weight of a fruit liquid consisting of apple, orange, carrot and aloe, 78.5 to 84.5% by weight of corn starch; And Fermentation medium manufacturing step comprising the step of combining the sugar cane 0.5 to 1.5% by weight; (Ii) inoculating the strain to inoculate Lactobacillus sp. And Saccharomyces sp. (Iii) fermenting for 70 to 80 hours at a temperature of 35 to 45 ° C. and a humidity of 95 ± 5% after inoculation of the strain; And, (Iii) drying the fermentation product at 70 to 75 ° C. for 60 to 72 hours to form a moisture content of less than 1 to 10%, The fruit solution is a method for producing an organic metabolite-containing biocomposite containing 3.34 parts by weight of apples and oranges, and about 20 parts by weight of aloe, based on 100 parts by weight of carrots. delete The method of claim 1, The composites are expected oil, characterized in that includes the Lactobacillus (Lactobacillus sp.) 1.0X10 ⁷ to 1.0X10 ⁸ cfu / g, saccharose in my process (Saccharomyces sp.) 1.0X10 ⁷ to 1.0X10 ⁸ cfu / g Method for producing bio-composite containing thing. The method of claim 1, The method of producing an organic metabolite-containing biocomposite, characterized in that the acidity of the fermentation medium is pH 2.5 to 3.5. An organic metabolite-containing biocomposite prepared by the method of claim 1. The method of claim 5, wherein the combination is Lactobacillus ( Lactobacillus) sp . ) 1.0 × ^{10 7} to 1.0 × ^{10 8} cfu / g, Saccharomyces sp . ) 1.0X10 ⁷ to 1.0X10 ⁸ cfu / g of the organic metabolite-containing biocomposite characterized in that it contains.

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