RU2433738C1 - Method for production of fodder additive for prevention of animals and birds mycotoxicosis - Google Patents

Abstract

FIELD: food industry. ^ SUBSTANCE: invention is intended for use in production of fodders for farm animals and birds. The cellulose containing substrate is represented by beet pulp or juices and wines production waste (i.e. refuses) milled into particles sized so that: remainder on the sieve is 2.0 no more than 15%, one adds to the substrate a preliminarily produced yeast (Saccharomyces cerevisiae) autolysate in an amount of 2.5-8.5 wt % per 1 t of the dry product and process water till moisture content is 18-47%, one proceeds with pasteurisation of the mixture at a temperature of 80C during 2-2.5 h, cooling to a temperature of 48-50C, then one performs fermentative treatment of the substrate with a cellulolytic enzyme during 2.5-4 h at a temperature of 48-50C, dries it till the mixture moisture content is 8-12% at a temperature no higher than 85C and adds to the produced mixture 15-45 wt % of Ferm KM probiotic fodder additive for farm animals. The fodder additive is produced by way of separate in-depth cultivation of strains Bacillus subtilis VKPM V-8130, Bacillus subtilis VKPM V-2984, Bacillus subtilis VKPM V-4099 and Bacillus licheniformis VKPM V-4162 and subsequent solid state cultivation of Bacillus subtilis liquid cultures based on beet pulp and wheat bran and drying till moisture content is 8-10%. Bacillus licheniformis VKPM V-4162 liquid culture is immediately mixed with beet pulp and dried. Then the dried cultures are mixed and milled till a homogeneous product formation. ^ EFFECT: invention ensures enhancement of activity display in deactivation of many mycotoxins, enhancement of animals and birds productivity due to fodder digestibility, fodder conversion increase by 10% and intensification of metabolic processes in the organism, livestock liveability enhancement. ^ 4 cl, 6 ex

Description

The invention relates to biotechnology and feed production, in particular to technologies for the preparation of animal feed containing special complex probiotic additives that prevent the toxicity of animals and birds through biotransformation, binding of toxins and the prevention of gastrointestinal disorders.

The current state of feed production is characterized by a high seed distribution of grain with a fairly wide range of phytopathogens, in particular fungi: many species of Aspergillus. Fusarium, Penicillium, Claviceps, Alternaria. Mycotoxins comprise a group of several hundred chemically dissimilar toxins. The most common are aflatoxins, ochratoxin A, trichothecenes, zearalenone, and fumonisans. Experts determine the proportion of grain infected with mycotoxins in the amount of 25-50%, which is undoubtedly dangerous to human and animal health. The increased frequency of feed poisoning, which occurs acutely and latently in large farms with a certain periodicity, has put on the agenda the organization of systemic preventive use of toxin sorbents in animal feed. Experts note that the chemistry and structure of toxins are well understood, but there are no sufficiently effective drugs for the prevention and treatment of mycotoxicoses.

With the emergence of the problem of the toxicity of animal feed in the conditions of industrial animal husbandry and poultry farming, the first sorbents were various compositions of mineral clays. Recent advances in the prevention of mycotoxicosis are associated with universal organic phytosorbents, which have high enzymatic biological activity, which provide biosecurity of the body from the harmful effects of toxins.

A known method for the complex use of the enzymatic probiotic Cellobacterin and Mikosorb, which is a combination of modified glucomannans isolated from the inner cell walls of specially selected strains of the yeast Saccharomyces cerevisiae (Alltech company), as a digestive stabilizer and adsorbent of mycotoxins in pigs, 10.05.2007 U2. .

A known method for producing a biologically active feed additive, comprising two stages of its preparation: deep aerobic cultivation of a probiotic cellulolytic bacillus - a strain of Bacillus, subtilis VKPM B-8130, which is part of the drug Probiocel - additives to animal feed, obtaining a liquid culture (first stage) , and subsequent solid-phase fermentation of plant materials with difficult access of oxygen (second stage). The process is carried out in the presence of sodium bicarbonate (NaHCO ₃ ) to bind acetyl-CoA and neutralize acidic fermentation products, thereby using the Neuberg metabolic trap method for yeast fermentation. As plant raw materials, sea buckthorn, dogrose, sunflower meal or cake are used, waste from flour milling, cereals production, RU 2346463 C2, 02.20.2009.

The disadvantages are the use of a monoculture of bacteria, which limits the spectrum of biologically active action of the drug on the animal organism, and the weak sorption ability of mycotoxins.

There is a method of producing a feed additive, which simultaneously with dry beet pulp additionally contains a probiotic, which includes bacteria cultures of Bifidobacterium globosum, Enterococcus faecium and Bacillus subtilis, fumaric acid and zeolite. This feed additive allows you to increase the consumption and improve the use of young pigs feed containing dry beet pulp, which helps to increase the average daily increase in live weight of young pigs and reduce the cost of pig production, but does not provide sufficient ability to sorb mycotoxins, RU 2359465 C1, 06.27.2009.

The closest in technical essence and the achieved result is a method of obtaining a feed additive for farm animals and poultry by solid-phase fermentation of beet pulp, including preliminary separate cultivation of a strain of Bacillus mucilaginosus VKPM B-5987 and a strain of Streptococcus faecium TE-17 (collection of JSC Bioflora B- 07) in a liquid nutrient medium, inoculation of beet pulp with a strain of Bacillus mucilaginosus VKPM B-5987, and then with a strain of Streptococcus faecium TE-17, with lactic acid, RU 2352136 C2, April 20, 2009.

The technical result of the invention is to increase the manifestation of activity in the deactivation of many mycotoxins, as well as increasing efficiency due to the content in the feed additive of a large volume of pectin, which has increased sorption ability and bacteriostatic effect. Along with this, an increase in the productivity of animals and poultry is ensured by increasing the digestibility of feed, increasing feed conversion by 10% and enhancing metabolic processes in the body, increasing the safety of the livestock.

The resulting feed additive is a universal organic phytosorbent, which effectively prevents the toxicity of animals and birds by binding toxins, their biotransformation and preventing the development of disorders in the gastrointestinal tract, increases the technical and economic indicators of livestock production, poultry meat and eggs.

According to this proposal, in the technology for the production of a feed additive, there is no stage of solid-phase fermentation, and the probiotic component of the feed additive is introduced as a constituent component on a specially prepared substrate having a frame of organic fiber of beet pulp or squeeze. Moreover, the essential distinguishing feature of the proposal is not the fact of the presence of a concentrate of probiotic cultures in the form of probiotic feed additives Ferm KM, but the features, parameters and initial product of special preparation of the substrate. Waste from plant production containing fiber and a large amount of pectin substances — beet pulp, waste from the production of juices and wines — squeezes, is used as a substrate. To effectively conduct the process of preparation of the substrate, it is preliminarily normalized to a particle size with a particle size characterized by a residue on sieve 2.0 of not more than 15%.

It has been established that it is the preparation of specific conditions for the activity of the complex of probiotic cultures represented by the probiotic feed supplement Ferm KM, namely the nutritional value of the medium, acidity of the medium, humidity, temperature, protection from external influences during digestion, which provide powerful manifestations of their known activity in the deactivation of many mycotoxins In addition to this, the high efficiency of the additive is ensured by the introduction of a large volume of pectin, which has increased sorption ability, into its composition Strongly and bacteriostatic action.

To achieve a technical result in the preparation of a feed additive for the prevention of mycotoxicosis in farm animals and poultry, the cellulose-containing substrate is crushed to a particle size with a particle size not exceeding 15% on a sieve, and the previously obtained Saccharomyces cerevisiae yeast autolysate is added in an amount of 2.5- 8.5 wt.% Per 1 ton of dry product and process water to a moisture content of 18-47%, pasteurization of the mixture is carried out at a temperature of 80 ° C for 2-2.5 hours, cooling to a temperature of 48-50 ° C, after which conduct the enzymatic treatment of the substrate with a cellulolytic enzyme for 2.5-4 hours at a temperature of 48-50 ° C, then dry to a moisture content of 8-12% at a temperature not exceeding 85 ° C and add 15-45 wt.% probiotic feed to the resulting mixture additives for farm animals Farm KM obtained by separate deep cultivation of strains of Bacillus subtilis VKPM B-8130 and Bacillus subtilis VKPM B-2984 on a nutrient medium containing, g / l:

molasses 24-26 corn gluten 14-16 corn extract 19-21 disubstituted potassium phosphate 1-3 sodium chloride 9-11 magnesium sulfate 0.4-0.6 water rest

strain Bacillus subtilis VKPM B-4099 on a nutrient medium containing, g / l:

molasses 14-16 corn gluten 9-11 corn extract 9-11 disubstituted ammonium phosphate 1-3 sodium chloride 9-12 magnesium sulfate 0.4-0.6 water rest

strain Bacillus licheniformis VKPM B-4162 in a nutrient medium containing, g / l:

molasses 24-26 corn extract 18-22 disubstituted potassium phosphate 1-3 sodium chloride 9-11 magnesium sulfate 0.4-0.6 water rest

for 18-24 hours, and subsequent solid-phase fermentation, for which the obtained liquid cultures of Bacillus subtilis VKPM B-8130, Bacillus subtilis VKPM B-2984 and Bacillus subtilis VKPM B-4099 are mixed in a 6: 6: 1 ratio, applied to a sterile mixture beet pulp and wheat bran, which is pretreated with a cellulolytic enzyme dissolved in a nutrient medium containing 30 l of water: molasses 255-265 g, potassium disubstituted 98-102 g, magnesium sulfate 25-26 g, yeast 1.8-2 , 2 g, adjust pH to 7.5-8.0 and kneading humidity to 43-48%, mix thoroughly They are solidified, they carry out solid-phase fermentation under conditions of limited access of oxygen for 48 hours at a temperature of 45-50 ° C and dried at a temperature of 50 ° C to a moisture content of 8-10%, and the liquid culture of Bacillus licheniformis VKPM B-4162 is directly mixed with beet pulp and dried to a moisture content of 8-9%, the obtained dried products are stirred for 0.5 h and subjected to crushing to obtain a homogeneous product. A method for producing a probiotic feed additive of KM Farms additionally provides for the introduction of a probiotic Lactovit containing cultures of Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium bifidum and / or Bifidobacterium longumobis lobis bumidobacterium bumidobacterium, and / or Bifidobacterium longumus and / or Bifidobacterium bumidocactum and / or Bifidobacterium bumidocactum and / or Bifidobacterium longum bacidus and / or Bifidobacterium longumobis bacidae and / or Bifidobacterium bumidocactum and / or Bifidobacterium longum bacidus and / or Bifidobacterium longumobacterium and / or Bifidobacterium longum bacidus cremoris or Lactococcus lactis subsp, Lactobacillus casei, Lactobacillus delbrueckii subsp. bulgaricus. Probiotic Lactovit for animals and poultry is produced by NTTs BIO LLC in the city of Shebekino, Belgorod Region. according to TU 9291-002-54664067-2003. As a cellulolytic enzyme, celloviridin or cellulox F is used with a cellulase content of at least 2000 u / g, and beet pulp is used as a cellulose-containing substrate, juice from the production of juices and wine is squeezed. The resulting feed additive is named DBA FITOS.

The invention is illustrated by the following examples.

Example 1

Beet pulp is chosen as a substrate. To the crushed beet pulp, the previously obtained autolysate of Saccharomyces cerevisiae yeast is added in an amount of 2.5-8.5 wt.% Per 1 ton of dry product and process water to a moisture content of 18-47%, the mixture is pasteurized at a temperature of 80 ° C for 2- 2.5 hours, cooling to a temperature of 48-50 ° C, after which the substrate is enzymatically treated with a cellulolytic enzyme for 2.5-4 hours at a temperature of 48-50 ° C, then it is dried to a moisture content of 8-12% at a temperature not higher than 85 ° C and to the resulting mixture add 15-45 wt.% Probiotic feed additives for farm animals KM Farm, which was obtained by separate deep cultivation of strains of Bacillus subtilis VKPM B-8130, Bacillus subtilis VKPM B-2984, Bacillus subtilis VKPM B-4099 and Bacillus subtilis VKPM B-4162 and subsequent solid-phase fermentation. Why the obtained liquid cultures of Bacillus subtilis VKPM B-8130, Bacillus subtilis VKPM B-2984 and Bacillus subtilis VKPM B-4099 are mixed in a 6: 6: 1 ratio, applied to a sterile mixture of beet pulp and wheat bran, which is pre-treated with a cellulolytic enzyme, dissolved in a nutrient medium containing 30 l of water: molasses 255-265 g, potassium disubstituted phosphate 98-102 g, magnesium sulfate 25-26 g, yeast 1.8-2.2 g. After which the pH is adjusted to 7.5 -8.0 and kneading moisture up to 43-48%, the mixture is thoroughly mixed. Solid phase fermentation is carried out under conditions of limited oxygen access for 48 hours at a temperature of 50 ° C, the resulting mixture is dried at a temperature of 50 ° C to a moisture content of 9%. At the same time, a liquid culture of Bacillus licheniformis VKPM B-4162 is directly applied to beet pulp, thoroughly mixed and dried to a moisture content of 9%. The resulting dried products are stirred for 0.5 h and subjected to crushing to obtain a homogeneous product.

The obtained feed additive DBA FITOS is used to prevent mycotoxicity of farm animals and birds by introducing it into feed rations at a dose of 1 kg / t by direct introduction into feed or entering it into premixes, BMVD, superconcentrates or other components of feed.

Example 2

The effectiveness of the use of DBA FITOS was tested on the collective farm to them. Frunze Belgorod region. During the tests, it was found that the use of the drug in a dose of 1 kg / t of feed for feeding weaned piglets made it possible to increase the average daily gain by 3.2-6.7%, reduce the toxic load and improve production performance.

Example 3

In OOO Vozrozhdenie of the Krasnogvardeisky district of the Belgorod Region, the use of FITOS DBA in feeding piglets at the stage of growing at a dose of 1 kg / t of feed provided an increase in average daily gain by 5.8% compared to control, which was closer to the performance on non-contaminated raw materials.

Example 4

In experiments conducted at Belgorod Broiler CJSC, feeding laying hens with compound feed with 1 kg / t feed additive DBA FITOS for 2 months allowed to increase egg production by 2.8%, reduce embryonic mortality by 3.7%. The results confirm the effectiveness of the drug when using feeds contaminated with mycotoxins due to the physical structure of the phytosorbent and the total charge of pectin substances, which are a multicharged structure and have a high sorption ability. The feed additive used for detoxification purposes also has protective properties due to the probiotic component, which is especially important when feed is infected with non-polar compounds that adversely affect the parent herd in laying hens, causing reproductive dysfunction.

Example 5

The introduction of the DBA FITOS feed additive into the feed feed of broiler chickens at Prioskolye CJSC (complete growing cycle of 39 days) in the amount of 1 kg / t of feed provided high preservation of the bird, had a positive effect on the use of nutrients, and increased the average daily gain by 1.5 - 2 , 8%, which led to an improvement in most production indicators. Reducing the negative burden from the action of mycotoxins in combination with the stimulating effect of the probiotic component ensured the normalization of metabolism and improved technical and economic indicators of broiler cultivation.

Example 6

The use of DBA FITOS in OOO Vozrozhdenie of the Krasnogvardeisky district of the Belgorod Region by introducing it into the diet of pregnant sows in the period 2 months before farrowing and up to 28 days after farrowing (before weaning piglets) in the amount of 1 kg / t of feed, provided a more healthy offspring piglets (increase in safety by 15%). The average weight of the pig increased by 4.3%.

Claims (4)

1. A method of obtaining a feed additive for the prevention of mycotoxicosis in animals and birds, which consists in crushing a cellulose-containing substrate to a particle size with a particle size of characterized by: a sieve residue of 2.0 no more than 15%, add the previously obtained Saccharomyces cerevisiae yeast autolysate to 2.5-8.5 wt.% per 1 ton of dry product and process water to a moisture content of 18-47%, pasteurization of the mixture at a temperature of 80 ° C for 2-2.5 hours, cooling to a temperature of 48-50 ° C, after which the enzymatic treatment cleaned with a cellulolytic enzyme for 2.5-4 hours at a temperature of 48-50 ° C, then dried to a moisture content of 8-12% at a temperature not exceeding 85 ° C and 15-45 wt.% probiotic feed additives for agricultural products are added to the resulting mixture farm animals KM obtained by separate deep cultivation of strains of Bacillus subtilis VKPM B-8130 and Bacillus subtilis VKPM B-2984 on a nutrient medium containing, g / l:
molasses 24-26 corn gluten 14-16 corn extract 19-21 disubstituted potassium phosphate 1-3 sodium chloride 9-11 magnesium sulfate 0.4-0.6 water rest,
strain Bacillus subtilis VKPM B-4099 on a nutrient medium containing, g / l:
molasses 14-16 corn gluten 9-11 corn extract 9-11 disubstituted ammonium phosphate 1-3 sodium chloride 9-12 magnesium sulfate 0.4-0.6 water rest,
strain Bacillus licheniformis VKPM B-4162 in a nutrient medium containing, g / l:
molasses 24-26 corn extract 18-22 disubstituted potassium phosphate 1-3 sodium chloride 9-11 magnesium sulfate 0.4-0.6 water rest,
for 18-24 hours, and subsequent solid-phase fermentation, for which the obtained liquid cultures of Bacillus subtilis VKPM B-8130, Bacillus subtilis VKPM B-2984 and Bacillus subtilis VKPM B-4099 are mixed in a 6: 6: 1 ratio, applied to a sterile mixture beet pulp and wheat bran, which is pretreated with a cellulolytic enzyme dissolved in a nutrient medium containing 30 l of water: molasses - 255-265 g, potassium disubstituted phosphate - 98-102 g, magnesium sulfate - 25-26 g, yeast - 1 , 8-2.2 g, adjust the pH to 7.5-8.0 and the humidity of the batch to 43-48%, the mixture is carefully transferred they are dried, solid phase fermentation is carried out under conditions of limited oxygen access for 48 hours at a temperature of 45-50 ° C and dried at a temperature of 50 ° C to a moisture content of 8-10%, and the Bacillus licheniformis VKPM B-4162 liquid culture is directly mixed with beet pulp and dried to a moisture content of 8-9%, the obtained dried products are stirred for 0.5 h and subjected to crushing to obtain a homogeneous product. 2. The method according to claim 1, characterized in that before solid-phase cultivation, Lactovit probiotic is additionally added to the obtained liquid cultures of Bacillus subtilis, containing the cultures of: Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium bifidum, and / or Bifidobacterium longum, and / or Adifoles, Lactococcus lactis subsp. cremoris or Lactococcus lactis subsp, Lactobacillus casei, Lactobacillus delbrueckii subsp. bulgaricus. 3. The method according to claim 1, characterized in that as cellulolytic enzyme use celloviridine or cellulox F with a cellulase content of at least 2000 units / g 4. The method according to claim 1, characterized in that beet pulp is used as a cellulose-containing substrate, waste juice and wine production waste.