KR101748368B1 - Fermentation feed for domestic animal comprising liquid fermented sulfuric - Google Patents

Abstract

The present invention relates to a method for the preparation of a medicinal product comprising the steps of: a) adding sulfur and mica and selenium to a medicinal extract comprising water and moss, licorice and fennel and mixing; b) low-temperature melting the mixture of step a) with stirring at a temperature of 38 to 45 DEG C for 45 to 60 days; c) mixing and heating molasses, glucose, sodium and water to the melt of step b); d) inoculating a heated fermentation broth of Lactobacillus casei and Acetobacter tropicalis into the heated product of step c), followed by fermentation to produce liquid fermented sulfur; And e) fermenting the liquid fermentation sulfur and the cereal by-products of step d) by inoculating a yeast culture liquid, and a method for producing the fermented feed for livestock containing liquid fermented sulfur, Fermented feeds reduce the mortality of livestock by liquid fermentation sulfur when fed to livestock, improve the amount of feed and feed rate when fed to livestock, and reduce liver damage enzyme, cholesterol and fat content. In addition, the growth of livestock can be promoted to reduce the production cost per unit, and the meat taste can be improved.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a fermentation feed for domestic animals containing liquid fermented sulfuric acid,

The present invention relates to a fermented feed for livestock containing liquid fermented sulfur, and more particularly to a fermented feed for livestock containing liquid fermented sulfur, more specifically, liquid fermented sulfur produced by using Lactobacillus casei and Acetobacter tropicalis as an active ingredient The present invention relates to a fermented feed for livestock.

Antibiotics in livestock are known to exhibit excellent effects such as promotion of livestock growth, improvement of feed efficiency and prevention of disease, and they have been continuously used as feed additives. However, due to resistance and persistence of antibiotics, a superbacter was developed that selectively resistant to antibiotics in human and domestic animals. In the environment, horizontal migration of antibiotic resistance genes, the emergence of methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and the emergence of antibiotic resistant bacteria in livestock food in hospitalized patients has become a serious social issue and since July 2011, antibiotics for growth promotion have been banned (Shakibaie et al, 2009, J. Envirion Biol, 30:... 45-49 .; Cosgrove et al, 2005, Epidemiol, 26:.. 166-174 .; Toroglu et al. , 2009, J. Envirion, Biol., 30: 23-31, Toroglu et al. , 2005, Ann. Microbiol., 55: 229-233.). Industrial animals have not been able to prevent disease or become less resistant to disease, resulting in significant productivity and economic damage in livestock farmers and related industries. Various attempts have been made to increase the resistance to diseases, such as natural antimicrobials and vaccines However, the effect is negligible. In the aftermath of this, the domestic livestock industry has suffered from many problems such as lower productivity and the collapse of small livestock farms. In other words, the development of alternative natural antimicrobial substances, immunostimulants, functional additives and metabolism accelerators to improve the productivity of direct livestock farmers and industries by solving problems such as improvement of the growth rate of industrial animals, reduction of mortality rate, It is essential in domestic livestock industry.

On the other hand, sulfur has been used as a remedy for various diseases such as constipation and chichil, both East and West, and sulfur compounds contained in garlic and onion extracts are widely used as medicines for diseases such as typhoid, cholera, (1998), "MSM" Feb / Mar, 20 (1): 30-31 . Wu et al. , 2005, Mutation Research., 89 , Kumar et al. , 1998, J. Appl. Microbiol., 84: 84-102 .; Lee et al. , 2008, Toxicology in Bitro., 22: 87-95 .; Lock, 1992, Feedstuffs, 19:18; : 213-215.).

These sulfur can not be produced naturally in the human body and should be replenished from the outside. The mineral sulfur can not be used for food because of the heavy metal component and toxicity mixed with it. Therefore, most of the animals (such as dung, (Ginger, duck meat, onion, onion, mustard, leek, ginseng, garlic, etc.). However, the amount of sulfur present in the plant is very small, most of which is difficult to be absorbed in the inorganic sulfur state, and it takes a lot of time and cost to ingest the sulfur present in the animal.

In the past, attempts have been made in the art to use legal sulfur as a feed additive in which the toxicity is reduced while maintaining the sulfur efficacy. However, the above-mentioned sulfur is not effective in decreasing the toxicity of sulfur. Therefore, when the animal is used as a feed for livestock, the mortality rate of the livestock should be 30% or more, and the amount of sulfur to be supplied should be limited. There is a problem that the effect is reduced. Therefore, in addition to a method of easily eliminating the toxicity of sulfur, various attempts have been made to produce feeds containing sulfur prepared according to the above method.

Korean Patent Laid-Open Publication No. 2010-0019914 discloses a method for producing soybean protein by inoculating a protein contained in soybean flour into a primary fermented product, adding sulfur to the fermented product, Korean Patent Registration No. 10-1334157 discloses a method for producing a feed for livestock containing sulfur eliminated with toxicity, wherein a toxic sulfur powder is added to a mixed microbial culture comprising lactic acid bacteria and yeast, Fermented and decomposed, followed by drying, to prepare a feed composition containing the decolorized sulfur fermentation powder. However, since the above method uses only fermenting microorganisms, it takes a long time to remove the toxicity of sulfur, and the fermentation time varies depending on the type of the fermenting microorganism, so that there is a problem that mass production of the feed can not be achieved through the automation process. In addition, when feeding to livestock, weight gain, flow rate and the like are mentioned, but the effect of decreasing the liver damage enzyme, cholesterol and fat content as in the present invention is not disclosed.

Accordingly, the present invention solves the above problems and attempts to produce a fermented feed that can improve the growth of livestock and the feed utilization without sulfur toxicity. Among the minerals and herbal extracts and lactobacillus casei Lactobacillus casei ) and Acetobacter tropicalis , and fermented feedstuffs prepared by inoculating yeast culture liquid after mixing the fermented liquid fermented by-products with cereal by-products, When fed to livestock, liquid fermentation sulfur improves resistance to bacteria or pathogens, resulting in reduced livestock mortality, improved weight gain and feed conversion rate, and reduced liver damage enzymes, cholesterol and fat content. And the present invention was completed.

It is an object of the present invention to provide a method for producing a fermented feed for livestock which can improve the growth and feed utilization of livestock without toxicity of sulfur.

It is another object of the present invention to provide a fermented feed for livestock produced according to the above production method.

In one aspect, the present invention provides a method of preparing a fermented feed for livestock, comprising the steps of:

a) adding sulfur, mica and selenium to the herbal extract, including water, mucilage, licorice and fennel, and mixing;

b) low-temperature melting the mixture of step a) with stirring;

c) mixing and heating molasses, glucose, sodium and water to the melt of step b);

d) inoculating a fermentation strain into the heated product of step c) and fermenting the fermented product to prepare a liquid fermented sulfur; And

e) mixing the liquid fermented sulfur of step d) with cereal by-products, and inoculating and fermenting yeast culture liquid.

The method for producing the fermented feed for livestock according to the present invention will be described in detail with reference to the following respective steps.

a) adding sulfur, mica and selenium to the herbal extracts, including water, mites, licorice and fennel, and mixing them.

Specifically, the step a) of the present invention is a step of adding sulfuric acid, mica and selenium to water to homogenize them, and then adding and mixing the herbal extracts including moss, licorice, and fennel.

In the present invention, the sulfur is soaked in water flowing through mineral sulfur, and is then rinsed with a brush to remove impurities, followed by drying and pulverization with 600 to 800 mesh.

In the present invention, the mica and selenium are used to inhibit the activity of a toxic substance by binding to a toxic substance such as heavy metals in sulfur. The mica and selenium are contained in an amount of 45 to 55 parts by weight, preferably 48 to 55 parts by weight, It is preferably used in an amount of 52 parts by weight. When the above-mentioned mica or selenium is used in an amount of less than 45 parts by weight, the content of toxic substances which can not be combined with mica or selenium is large, so that the fermented feeds of the present invention can be produced. When mica or selenium is used in an amount exceeding 55 parts by weight The activity of the sulfur poisonous substance is inhibited, but the secondary problem such as environmental destruction and disease induction may occur due to toxicity of mica or selenium.

At this time, the mica or selenium is soaked in flowing water, rubbed with a brush, and then dried to remove foreign matters, and the powder is pulverized to 600 to 800 mesh.

In the present invention, the medicinal plant extract containing the mites, licorice, and fennel is used for removing toxic substances not removed by mica and selenium and at the same time improving the storage stability of the feed. It is preferable to use a solvent which has been extracted with a conventional solvent known in the art and then concentrated to a concentration of 48 to 52% by weight of the solvent of the extract based on the total weight of the extract. Preferably, 1 to 3 parts by weight, 1 to 3 parts by weight, and 0.5 to 1.5 parts by weight of the above-mentioned mites, licorice and fennel are used relative to 100 parts by weight of the solvent.

The solvent may be, for example, (a) water, (b) an anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohol and water, d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol as extraction solvents. Preferably, extraction is carried out using water, methanol or ethanol. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the extract may differ. Therefore, an appropriate organic solvent should be selected and used. The extraction method is not particularly limited, and examples thereof include hot water extraction, cold extraction, ultrasonic extraction, and reflux cooling extraction.

The concentration may be any method for removing the solvent in the extract to increase the concentration of the extract, for example, heating, concentration under reduced pressure, concentration by gust, freezing concentration, and spray concentration.

The fungus, licorice, and fennel are readily available to those skilled in the art. Commercially available products may be used or they may be cut or cut into a certain size and roasted, preferably roasted after cutting. This is to allow each component to leach well into the solvent depending on the properties of each material.

In the present invention, it is preferable that the medicinal plant extract containing the mites, licorice and fennel is used in an amount of 45 to 55 parts by weight, preferably 48 to 52 parts by weight, based on 100 parts by weight of sulfur.

The efficacy of the medicinal materials used for producing the liquid fermentation sulfur of the present invention will be described below.

Eucommia ulmoides Oliver is a dried bark and contains gutta-percha, glycosides, alkaloids, pectin, resin, organic acid, cast, vitamin C and chlorogenic acid. Anti-aging, cholesterol lowering, anti-inflammation, immunity enhancement, cleansing effect in the body, antibacterial activity and antiallergic.

Licorice ( Glycyrrhiza uralensis Fischer) is a plant belonging to the family Rosaceae leguminosae, and contains flavonoids such as glycyrrhizin, liquiritigenin and lliquiritin, Antimicrobial, anti-inflammatory, anti-cancer, and poisoning by drugs and plants.

Fennel is a dried fruit of the mature fruit of Feniculum vulgare Miller and contains essential oil components such as anethole, fenchone, piene and limone, And strengthen the above functions, and is said to be effective in the emission of harmful substances and antibacterial.

and b) low-temperature melting the mixture of step a) with stirring.

Specifically, step (b) of the present invention is characterized in that the mixture of step (a) is stirred at a temperature of 38 to 45 DEG C for 45 to 60 days. If the stirring is less than 38 ° C or less than 45 days, the mixture is not melted and it is difficult to obtain a liquid mixture. If stirring is performed at more than 45 ° C or more than 50 days, a separate heating device It is costly to manufacture a fermented feed, which is uneconomical.

At this time, it is preferable that the melting is performed in a closed container which is easy to handle poison, for example, an aluminum container, a stainless container, or the like. Here, the enclosed space means a space blocked from external air or foreign matter.

The low-temperature melting process has an advantage that the sulfur can be changed into a liquid state and at the same time, an environment suitable for the growth of the microorganisms contained in the medicinal material can be produced, so that a physiologically active substance can be obtained from the medicinal material without a separate microbial fermentation process.

c) mixing molasses, glucose, sodium and water to the melt of step b) and heating.

Specifically, in step c), 90 to 110 parts by weight of molasses, 40 to 60 parts by weight of glucose, 20 to 30 parts by weight of sodium and 4,000 to 6,000 parts by weight of water are mixed with the melt of step b) Followed by heating at a temperature of 90 to 110 DEG C for 60 to 70 minutes.

This heating step volatilizes the mucous or selenium-bonded toxic substances in step a) to remove the toxic substances contained in the sulfur and can kill germs or pathogens present in the molasses or glucose. Therefore, a separate antibiotic There is an advantage that second pollution can be prevented without using it.

d) Inoculating a fermentation strain into the heated product of step c), followed by fermentation to produce liquid fermented sulfur.

In the present invention, the strain is characterized in that the strain is a mixed strain in which Lactobacillus casei and Acetobacter tropicalis are mixed at a weight ratio of 1: 1.

The Lactobacillus casei is a fermented lactic acid bacterium that fermentes a saccharide to produce acetic acid, propionic acid, ethyl alcohol, carbonic acid gas and the like together with lactic acid. The growth rate of the lactic acid bacteria is minimized when the fermentation period of liquid sulfur is minimized have.

The acetonitrile bakteo trophy faecalis (Acetobacter tropicalis) is the b) with the separated chosangyun in the molten sulfur phase, acetonitrile bakteo trophy faecalis ^{(Acetobacter tropicalis) NRIC 0312 T (} AB032354) with more than 90%, preferably at least 95%, More preferably 98% or more homologous. It is excellent in the effect of lowering the pH of liquid sulfur as compared with other lactic acid bacteria by producing acetic acid from molasses or glucose and inhibiting the proliferation of perishable bacteria can do.

The Lactobacillus casei or Acetobacter tropicalis of the present invention can be obtained by inoculating each strain into a culture medium for the strain itself or a subculture culture and using the culture medium in which the strain has been grown, And the strain is inoculated with each of the strains in the culture medium. As one example, the Lactobacillus casei or Acetobacter tropicalis may be selected from the group consisting of Lactobacillus casei UPRO 02 (KCCM 10937P) or Acetobacter tropicalis NRIC 0312 ^T (MRS broth: 10 g / L of peptone, 10 g / L of beef extract, 5 g / L of yeast extract, 10 g / L of dextrose, 3 g / L of diammonium-citrate in a high pressure sterilized MRS broth , sodium acetate 5 g / L, tween 80 1 ml, K ₂ HPO ₄ 2 g / L, MgSO ₄ ㅇ 7H ₂ O 0.2 g / L, MnSO ₄ ㅇ 7H ₂ O 0.2 g / L, pH 6.2-6.6) And cultured at a temperature of 20 to 40 DEG C for 48 hours with stirring.

In the present invention, the fermentation is carried out by inoculating the fermentation strain of the same weight as the heated product into the heated product of the step c), and then heating the fermentation strain at 28 to 40 캜, preferably 30 to 40 캜, more preferably 35 to 40 캜 Deg.] C for 65 to 80 hours, preferably 68 to 75 hours.

The liquid fermented sulfur produced as described above has an excellent antimicrobial activity which completely removes toxic substances of sulfur and inhibits the growth of bacteria or pathogens.

e) mixing the liquid fermented sulfur in step d) with the by-product of the cereal, and then inoculating the fermented yeast culture solution.

Specifically, in step e) of the present invention, the liquid fermentation sulfur and the by-product of step d) are mixed at a weight ratio of 1: 4 to 6, preferably 1: 5, and the yeast culture solution is inoculated and fermented This process can improve the feed utilization of livestock by degrading the fibrous and degradable proteins contained in cereal by-products.

In the present invention, the yeast culture broth is a microorganism that degrades the fibers of cereal by-products, and it is preferable to use 0.05 to 0.2 parts by weight, preferably 0.1 parts by weight, based on 100 parts by weight of the mixture of liquid fermented sulfur and cereal by-products Do. When the yeast culture solution is used in an amount of less than 0.05 part by weight, decomposition of the fiber is not performed, and digestibility is significantly reduced when the yeast culture solution is added to the livestock. When the yeast culture solution is used in an amount exceeding 0.2 part by weight, Is reduced.

In the present invention, the cereal by-products may be any of those used for the use of livestock such as barley, rice, wheat, rye, oats, corn, rice, beans, peas, sorghum, Soybean, rice, and the like.

The animal is an animal capable of breeding using the fermented feed according to the present invention. Examples of the animal include rabbits such as pigs, goats, horses, cows, guinea pigs, etc., birds such as chickens, ducks, hummingbirds, , Carnivorous insects such as beards, monitors, and geckos, reptiles such as snakes, lizards, lizards, and chameleons, frogs, toads, salamanders, and amphibians or fish such as skates, salmon, dome, codfish and crucian carp.

In another aspect, the present invention provides a fermented feed for livestock produced according to the above method.

In the present invention, when the fermented feed for livestock is fed to livestock, the resistance against bacteria or pathogens is enhanced by the liquid fermentation sulfur, so that the mortality rate of the livestock is reduced, the amount of feed and the feed rate are improved, and the liver damage enzyme, cholesterol And an effect of reducing the fat content. In addition, the growth of livestock can be promoted to reduce the production cost per unit, and the meat taste can be improved.

The fermented feed for livestock produced according to the present invention reduces the mortality of livestock by liquid fermented sulfur when fed to livestock and improves the amount of feed and feed ratio when fed to livestock and improves the liver damage enzyme, . In addition, the growth of livestock can be promoted to reduce the production cost per unit, and the meat taste can be improved.

1 is a graph showing the pH of liquid sulfur measured during fermentation according to the kind of fermenting microorganism.

Hereinafter, the present invention will be described in more detail by way of examples and the like. It will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples and the like according to the gist of the present invention. will be.

Example 1: Production of liquid sulfur

1-1. Mixing step

1000 kg of yellow sulfur as a bonsai, 500 kg of mica, 500 g of selenium and 1500 L of water were mixed, and then 20 kg of mulberry, 20 kg of licorice and 10 kg of fennel were added to 1000 L of water and the boiled extract was concentrated to 500 L and mixed.

1-2. Melting step

The mixture of 1-2 was put in an aluminum or stainless steel container, sealed, and melted at 40 DEG C for 50 days with stirring.

1-3. Liquid sulfur production step

A mixture of sulfur, molasses, glucose, sodium, and water in a molar ratio of 1: 1: 0.5: 0.25: 50 was added to a stainless steel vessel, and air was blocked. The mixture was heated at 100 ° C. for 1 hour, .

Example 2: Selection of fermenting microorganism species

To select microorganisms capable of shortening the fermentation period of liquid sulfur, 100 mL of the preculture prepared in Example 1 was added with Lactobacillus reuteri , Pediococcus lolli , Lactobacillus platarium 5 ml of Lactobacillus plantarum , Lactobacillus casei or Acetobacter tropicalis were inoculated and cultured at 37 ° C for 20 days. The cells were cultured at 2-day intervals using a pH meter (M503P meter, wrks, Medifield , MA, USA). At this time, the Acetobacter tropicalis was isolated from the molten sulfur of Example 1-2, and was a strain showing 99% homology with Acetobacter tropicalis NRIC 0312 (T). As a control (control), liquid sulfur not inoculated with microorganisms was used. The results are shown in Fig.

As shown in FIG. 1, the pH of the treated group was lower than that of the control group at the initial stage of microbial inoculation. On the first day of fermentation, the pH of the treated group inoculated with Lactobacillus casei was lowest, It was confirmed that the pH of the group treated with Acetobacter tropicalis was lowest.

In the present invention, Lactobacillus casei and Acetobacter tropicalis were selected as microorganisms fermenting liquid sulfur, and they were mixed at a weight ratio of 1: 1.

Example 3: Production of sulfur fermented feed containing liquid fermented sulfur

Lactobacillus casei UPRO 02 (Lactobacillus casei UPRO, KCCM 10937P) and acetonitrile bakteo trophy faecalis ^{NRIC 0312 T (cetobacter tropicalis NRIC 0312} T) and 99% of a strain culture solution representing Bi 1: a microorganism mixture with 1 Example 1 to 1: 1 liquid sulfur, followed by primary fermentation at 37 ° C for 72 hours to prepare liquid fermented sulfur. Then, the liquid fermented sulfur and the cereal by-products were mixed, and the fermented milk was fermented by inoculating the yeast culture solution at 38 ° C for 72 hours for secondary fermentation. At this time, the mixing ratios of the liquid fermented sulfur, the by-products of the cereals, and the yeast culture liquid are shown in Table 1 below.

content(%) Wheat bran 16.65 Corn gluten 16.65 Rice bran 49.95 Fermented sulfur 16.65 Yeast culture (yeast culture) 0.10

Test Example 1: Analysis of components of fermented milk produced according to the present invention

According to the AOAC (2000) method, the moisture of the crude fermented feed prepared in Example 3 was subjected to the atmospheric pressure heat drying method (125 ° C., 3 hours), the crude protein was measured by the kjeldahl nitrogen determination method (N ㅧ 6.25) Respectively. Amino acid content was analyzed by Ninhydrin method using amino acid analyzer (Sykam amino acid analyzer S433, Germany). The number of yeast cells was counted by counting colonies formed by culturing on YGC agar. The results are shown in Table 2.

Chemical composition content Moisture (%) 6.46 Crude protein (%) 18.05 Crude fiber (%) 8.67 Crude ash,% 18.46 Yeast (CFU / g) 1.0 x 10 ² Amino acid content (%) Methionine 0.40 Cysteine 0.26 Tryptophan 0.09 Threonine 0.64 Cool 0.80 Proline 1.15 Valine 0.86 Iso-leucine 0.64 Leucine 1.46 Tyrosine 0.54 Lysine 0.46 Glycine 0.79 Alanine 0.95 Arginine 1.00 Glutamic acid 3.12 Aspartic acid 1.37 Histidine 0.35 Phenylalanine 0.81

Test Example 2: Analysis of physicochemical properties of broiler chickens fed with sulfur fermented feed of the present invention

2-1. Disclosure Animal and Test Design

The animals were 48,0121 Ross broiler (1 day old) produced in the hatchery in Damyang. The experimental design was carried out on May 19, 2015, with 25.986 control and 25.01 kcal fermented diets fed with commercial mixed feed (CP 18.5%, ME 3,250 Kcal / kg) and 0.2% sulfur fermented feed And paid for a total of 34 days until June 22, 2015.

2-2. Measurement of body weight gain, feed intake, feed conversion rate and mortality of broiler chicks fed with fermented milk

The weight gain of broiler chickens fed with sulfur fermented diets was measured by measuring body weight at the beginning and at the end of the test and by subtracting body weight at the start of body weight at the end. The feed intake ratio was calculated by subtracting the residual amount from the feed amount during the period before the test, and the feed conversion ratio was determined by dividing the feed intake by the amount of gain. The mortality rate according to the feeding of sulfur fermented diets was measured daily by the number of dead broiler chicks, divided by the number of broiler chicks in broiler count at the start and expressed as a percentage. The results are shown in Table 3.

Control group Sulfur fermented feed group Starting weight (g) 45 45 End of test Weight (g) 1,700 1,820 Weight gain (g) 1,655 1,775 Feed intake (g) 2,856 2,898 Feed rate (Gain) 1.73 1.63 Mortality (%) 2.14 2.06

As shown in Table 3, the amount of milk fed fermented milk was higher than that of the control group by 1,775 g in the fermented milk feed group, and the feed intake was 2,898 g in the fermented milk feed group, which was higher than that of the control group. Feeding rate and mortality were lower in the fermented milk fed group than in the control group, indicating that feeding of fermented milk was effective in improving feed efficiency and productivity.

2-3. Analysis of fecal microorganism characteristics of broiler chickens fed with sulfur fermented feed

The fecal microorganism of broiler chickens fed with sulfur fermented diets was sampled at the 0th, 3th, and the end of the test, and the feces were collected and sterilized in sterilized physiological saline, homogenized, ≪ / RTI > for 48 hours. The number of microorganisms in the feces was measured by counting the total bacteria, Salmonella, Escherichia coli, lactic acid bacteria and yeast. Total bacterial counts were counted by culturing on TSA agar. Salmonella assay was performed on XLT4 agar and black colonies were counted. Escherichia coli was cultured on Mac conkey agar to count red colonies. Lactic acid bacteria were cultured on MRS agar supplemented with BCP, Yellowish colonies were counted and yeast counts were determined by counting white colonies cultured on YGC agar. The results are shown in Table 4.

Control group Sulfur fermented feed group SEM P value 7days Total number of bacteria 8.02 7.90 0.62 0.7239 Lactobacillus
( Lactobacilli) 7.59 8.13 0.43 0.4855 Yeast 3.75 4.11 0.45 0.1394 E. coli 4.64 5.24 0.26 0.8106 Salmonella
(Salmonella sp.) 3.73 4.96 0.19 0.1477 14days Total number of bacteria 8.20 7.64 0.23 0.2312 Lactobacillus
( Lactobacilli) 8.04 ^a 6.95 ^b 0.09 0.0012 Yeast 4.69 5.81 0.47 0.1908 E. coli 4.56 4.13 0.31 0.4645 Salmonella
(Salmonella sp.) 0.00 ^b 2.64 ^a 0.25 0.0061 34days Total number of bacteria 8.30 8.13 0.22 0.6368 Lactobacillus
( Lactobacilli) 7.15 7.56 0.10 0.1214 Yeast 4.20 5.69 0.80 0.2800 E. coli 4.01 3.58 0.31 0.5188 Salmonella
(Salmonella sp.) 0.67 0.00 0.33 0.3700

As shown in FIG. 4, the number of lactic acid bacteria in broiler chicks fed sulfur fermented diets was the highest in the control group at the 14th day of fermented milk fed diet ( P <0.05) , But there was no significant difference in treatment interval. There was no significant difference between the control group and the fermented feed group during the measurement period, but the total number of bacteria was higher in the control group and the yeast was higher in the fermented milk feed group.

There was no significant difference between coliform and salmonella in the control and sulfur fermented feed groups, and the number of E. coli and Salmonella bacteria tended to decrease with time.

2-4. The blood characteristics of broiler chickens fed with sulfur fermented feed

At the end of the experiment, 3 rats were randomly selected from the control group and the fermented feed group at the end of the experiment, 2 ml of blood was collected from the venous blood, transferred to a vacutainer, and centrifuged at 5,000 rpm for 30 minutes. Respectively. (AST, ALT), total cholesterol, LDL cholesterol, and HDL cholesterol levels were measured using an automated blood analyzer (COBAS MIRA plus, ROCHE diagnostics) Respectively. The results are shown in Table 5.

Control group Sulfur fermented feed group SEM P value AST (IU / L) 336.67 ^a 278.33 ^b 8.36 0.0176 ALT (IU / L) 7.67 8.33 0.45 0.4216 Total cholesterol (mg / dL) 150.00 ^a 123.00 ^b 3.02 0.0062 LDL cholesterol (mg / dL) 32.33 ^a 19.00 ^b 0.83 0.0013 HDL cholesterol (mg / dL) 108.67 ^a 89.67 ^b 1.82 0.0048

As shown in Table 5, AST was lower in the fermented milk feed group than in the control group, but ALT was higher in the fermented milk feed group than in the control group. Considering the values of AST and ALT, the sulfur fermented feed is considered to be toxic to liver and kidney.

Total cholesterol, LDL cholesterol and HDL cholesterol were lower in the fermented milk fed group than in the control group.

2-5. Analysis of general components in broiler chicks fed with sulfur fermented feed

Immediately after the type of the test described in 2-1 above, broiler chicks close to the average weight were selected from each treatment group, and the weight of the shank meat and chest meat after the unhairing treatment was individually extracted at a ratio of 1: 1 and pulverized with a grinder to obtain an analytical sample Moisture, crude protein, crude fat and crude ash, and cholesterol content were analyzed and commissioned by the Agricultural Technology Application Foundation. The results are shown in Table 6.

Control group Sulfur fermented feed group SEM P value Moisture (%) 72.29 73.68 0.40 0.0740 Crude protein (%) 22.39 ^a 20.88 ^b 0.27 0.0318 Crude fat (%) 5.03 ^a 4.10 ^b 0.16 0.0170 Crude ash,% 1.00 0.97 0.04 0.5574 cholesterol
(Cholesterol, mg / 100g) 75.54 70.34 3.17 0.3246

As shown in Table 6, the protein and fat contents in chicken meat were lower in the fermented milk fed group than in the control group, and the cholesterol content was not significantly different between the control and the fermented milk fed group, Respectively.

2-6. Fatty acid composition of broiler chickens fed with sulfur fermented diets

Immediately after the type of specimen test described in 2-1 above, broiler chicks close to the average body weight were selected from each treatment group, and the weight of shank meat and chest meat was extracted at a ratio of 1: 1 after unhairing treatment and 1 g of each sample was pulverized with a grinder Fatty acid composition was analyzed using a gas chromatograph (HP 7890, Agilent Technologies, USA) in the Department of Animal Resources Science, Sunchon National University. The results are shown in Table 7.

Control group Sulfur fermented feed group SEM P value C12: 0 (Lauric acid) 0.03 0.02 0.02 0.7449 C14: 0 (Myristic acid) 1.00b 1.12a 0.02 0.0311 C15: 0 (Pentadecanoic acid) 0.07 0.00 0.02 0.1161 C16: 0 (Palmitic acid) 23.46 24.34 0.34 0.1481 C18: 0 (Stearic acid) 7.74a 7.06b 0.13 0.0211 C20: 0 (Arachidic acid) 0.69 0.71 0.02 0.4100 C21: 0 (Heneicosonoic acid) 0.17 0.19 0.01 0.6520 C14: 1 (Tetradecenoenoic acid) 0.34b 0.41a 0.01 0.0022 C16: 1 (Palmitoleic acid) 5.86b 7.00a 0.12 0.0027 C18: 1 (Oleic acid) 43.50 42.60 0.50 0.2745 C18: 1 trans (Trans oleic acid) 0.35 0.37 0.02 0.4676 C24: 1 (Nervonic acid) 0.31 0.31 0.05 0.9392 C18: 2 n-6 (Linoleic acid) 14.18 13.85 0.49 0.6625 C18: 3 n-3 (Linolenic acid) 0.21 0.39 0.06 0.2282 C20: 2 n-6 (11,14-Eicosadienoic acid) 0.15 0.20 0.02 0.2434 C20: 3n-6 (Eicosatrienoic acid) 0.39 0.42 0.07 0.7974 C20: 4 n-6 (Arachidonic acid) 1.62 1.52 0.26 0.8289 SFA 33.17 33.43 0.45 0.7220 UFA 50.35 50.70 0.28 0.6964 PUFA 16.56 16.39 0.76 0.8841 UFA / SFA 1.52 1.52 0.04 0.8615 Total n3 0.21 0.39 0.06 0.2241 Total n6 / n3 16.35 16.00 0.81 0.7823

As shown in Table 7, oleic acid, which is the highest fatty acid in the meat, showed 43.50% in the control group and 42.60% in the fermented milk feed group, but no statistically significant difference was found in the treatment period.

Linoleic acid, which is the highest content of essential fatty acids, showed 14.18% in control group and 13.85% in sulfur fermented feed group, but there was no statistically significant difference between treatments.

Tetradecenenoic acid and palmitoleic acid, which are unsaturated fatty acids, showed higher contents in the fermented milk feed group than in the control group.

Claims (8)

a) adding sulfur, mica and selenium to the herbal extract, including water, mucilage, licorice and fennel, and mixing; b) low-temperature melting the mixture of step a) with stirring at a temperature of 38 to 45 DEG C for 45 to 60 days; c) mixing the melt of step b) with molasses, glucose, sodium and water and heating at a temperature of 90 to 110 ° C for 60 to 70 minutes; d) inoculating a heated fermentation broth of Lactobacillus casei and Acetobacter tropicalis into the heated product of step c), and fermenting the fermented product to prepare a liquid fermented sulfur; And e) mixing the liquid fermentation sulfur of step d) and the by-products of cereals at a weight ratio of 1: 4 to 5, and then inoculating and fermenting the yeast culture broth to produce a fermented feed for livestock containing liquid fermented sulfur Way.
The method according to claim 1,
Wherein the step a) comprises adding 45 to 55 parts by weight of mica and selenium to the sulfur to 100 parts by weight of sulfur on the basis of 100 parts by weight of sulfur, homogenizing the mixture and then adding 45 to 55 parts by weight of bivalvia, licorice and fennel, based on 100 parts by weight of sulfur, Wherein the liquid fermented sulfur-containing fermented feed is added to and mixed with a pharmacopoeial extract.
The method according to claim 1,
The medicinal plant extracts containing bivalves, licorice and fennel in step a) are prepared by mixing 1 to 3 parts by weight, 1 to 3 parts by weight and 0.5 to 1.5 parts by weight of bivalves, licorice and fennel, respectively, with respect to 100 parts by weight of the extraction solvent Next, a method for producing a fermented feed for livestock containing liquid fermented sulfur, characterized in that it is prepared by concentrating the solvent of the extract liquid to 48 to 52% by weight based on the total weight of the extract liquid.
The method according to claim 1,
In step c), 90 to 110 parts by weight of molasses, 40 to 60 parts by weight of glucose, 20 to 30 parts by weight of sodium and 4,000 to 6,000 parts by weight of water are mixed with the melt of step b) based on 100 parts by weight of the melt Wherein the liquid fermentation sulfur is contained in the liquid fermented feed.
The method according to claim 1,
The fermenting feed for livestock according to claim 1, wherein the fermentation broth of step (d) is a mixture of Lactobacillus casei and Acetobacter tropicalis in a weight ratio of 1: 1. &Lt; / RTI &gt;
The method according to claim 1,
Wherein the fermentation in step d) is carried out at a temperature of 28 to 40 DEG C for 65 to 80 hours after the fermentation strain of the same weight as that of the heated product is inoculated into the heated product of step c) A method for producing a fermented feed.
The method according to claim 1,
The fermentation of step (e) is performed by inoculating 0.05 to 0.2 part by weight of yeast culture solution with respect to 100 parts by weight of the mixture of the mixture of liquid fermented sulfur and cereal by-products, at a temperature of 28 to 40 DEG C for 65 to 80 hours Wherein the liquid fermentation sulfur is contained in the liquid fermented feed.
A fermented feed for livestock produced according to the method of any one of claims 1 to 7.

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