KR20170051592A - Fermentation feed for domestic animal comprising liquid fermented sulfuric - Google Patents
Fermentation feed for domestic animal comprising liquid fermented sulfuric Download PDFInfo
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- KR20170051592A KR20170051592A KR1020150151064A KR20150151064A KR20170051592A KR 20170051592 A KR20170051592 A KR 20170051592A KR 1020150151064 A KR1020150151064 A KR 1020150151064A KR 20150151064 A KR20150151064 A KR 20150151064A KR 20170051592 A KR20170051592 A KR 20170051592A
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- sulfur
- weight
- fermented
- parts
- liquid
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
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Abstract
Description
본 발명은 액상발효유황이 함유된 가축용 발효사료에 관한 것으로, 보다 구체적으로는 락토바실러스 카제이(Lactobacillus casei) 및 아세토박터 트로피칼리스(Acetobacter tropicalis)를 이용하여 제조된 액상발효유황을 유효성분으로 포함하는 가축용 발효사료에 관한 것이다. The present invention relates to a fermented feed for livestock containing liquid fermented sulfur, and more particularly to a fermented feed for livestock containing liquid fermented sulfur, more specifically, liquid fermented sulfur produced by using Lactobacillus casei and Acetobacter tropicalis as an active ingredient The present invention relates to a fermented feed for livestock.
가축에서 항생제는 가축의 성장촉진, 사료효율 개선 및 질병 예방 등의 뛰어난 효과를 나타내는 것으로 알려져 사료 첨가제로 지속적으로 사용되고 있었다. 그러나 항생제의 내성 및 잔류문제로 사람과 가축의 생체에서 선택적으로 항생제에 대한 내성을 나타내는 강력한 세균(super bacteria)이 출현하였다. 환경에서는 항생제 내성유전자의 수평적인 이동, 병원에 입원중인 환자에서 메치실린 저항성 황색포도상구균, 반코마이신 내성 장구균의 출현 및 가축 식품에서 항생제 내성균의 출현은 심각한 사회적 현안이 되어 2011년 7월 이후 배합사료 내 성장촉진용 항생제 첨가가 전면 금지되었다(Shakibaie et al., 2009, J. Envirion. Biol., 30:45-49.; Cosgrove et al., 2005, Epidemiol., 26:166-174.; Toroglu et al., 2009, J. Envirion. Biol., 30:23-31.; Toroglu et al., 2005, Ann. Microbiol., 55:229-233.). 산업동물의 경우 질병을 예방하지 못하거나 질병에 대한 저항성이 낮아져 가축 농가나 관련 산업체에서는 생산성 및 경제적으로 큰 피해를 보았고, 질병에 대한 저항성을 높이기 위해 천연 항균물질이나 백신 요법 등 다양한 노력들이 시도되고 있으나, 그 효과는 미미한 실정이다. 그 여파로 국내 가축업은 생산성 저하 및 소규모 가축농가의 몰락 등 많은 문제점을 가져왔다. 즉, 산업동물의 증체율 향상, 폐사율 감소 및 치료비용 감소 등과 같은 문제를 해결하여 직접적인 가축농가 및 산업체의 생산성 증대에 도움을 주기 위해서는 대체 천연 항균물질이나 면역 증강제 또는 기능성 첨가제 및 대사 촉진제 등의 개발이 국내 가축업 영역에서 필수적으로 요구되고 있는 실정이다.Antibiotics in livestock are known to exhibit excellent effects such as promotion of livestock growth, improvement of feed efficiency and prevention of disease, and they have been continuously used as feed additives. However, due to resistance and persistence of antibiotics, a superbacter was developed that selectively resistant to antibiotics in human and domestic animals. In the environment, horizontal migration of antibiotic resistance genes, the emergence of methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and the emergence of antibiotic resistant bacteria in livestock food in hospitalized patients has become a serious social issue and since July 2011, antibiotics for growth promotion have been banned (Shakibaie et al, 2009, J. Envirion Biol, 30:... 45-49 .; Cosgrove et al, 2005, Epidemiol, 26:.. 166-174 .; Toroglu et al. , 2009, J. Envirion, Biol., 30: 23-31, Toroglu et al. , 2005, Ann. Microbiol., 55: 229-233.). Industrial animals have not been able to prevent disease or become less resistant to disease, resulting in significant productivity and economic damage in livestock farmers and related industries. Various attempts have been made to increase the resistance to diseases, such as natural antimicrobials and vaccines However, the effect is negligible. In the aftermath of this, the domestic livestock industry has suffered from many problems such as lower productivity and the collapse of small livestock farms. In other words, the development of alternative natural antimicrobial substances, immunostimulants, functional additives and metabolism accelerators to improve the productivity of direct livestock farmers and industries by solving problems such as improvement of the growth rate of industrial animals, reduction of mortality rate, It is essential in domestic livestock industry.
한편, 유황(sulfur)은 예로부터 동·서양을 막론하고 변비, 치칠 등의 여러 질병의 치료제로 쓰여 왔고, 마늘과 양파 추출물에 함유되어 있는 유황화합물은 티푸스, 콜레라, 이질 등 질병의 치료약으로 널리 사용되었으며, 병원성 미생물의 생육을 억제하는 효능이 있다고 알려져 있다(Total Health, 1998, "MSM" Feb/Mar, 20(1):30-31.; Wu et al., 2005, Mutation Research., 89:81-102.; Lee et al., 2008, Toxicology in Bitro., 22:87-95.; Lock, 1992, Feedstuffs, 19:18; Kumar et al., 1998, J. Appl. Microbiol., 84:213-215.). On the other hand, sulfur has been used as a remedy for various diseases such as constipation and chichil, both East and West, and sulfur compounds contained in garlic and onion extracts are widely used as medicines for diseases such as typhoid, cholera, (1998), "MSM" Feb / Mar, 20 (1): 30-31 . Wu et al. , 2005, Mutation Research., 89 , Kumar et al. , 1998, J. Appl. Microbiol., 84: 84-102 .; Lee et al. , 2008, Toxicology in Bitro., 22: 87-95 .; Lock, 1992, Feedstuffs, 19:18; : 213-215.).
이러한 유황은 인체 내에서 자연적으로 생성되지 않아 외부에서 보충해주어야 하는데, 광물성 유황은 함께 섞여 있는 중금속 성분과 독성 때문에 식용으로는 사용할 수 없어 대부분 동물(녹각, 웅담, 사향, 동물의 쓸개 등) 또는 식물(생강, 더덕, 파, 양파, 갓, 부추, 인삼, 마늘 등)을 섭취함으로써 보충한다. 그러나, 상기 식물에 존재하는 유황의 양은 매우 적고, 대부분 무기유황 상태로 흡수가 어려우며, 상기 동물에 존재하는 유황을 섭취하기 위해서는 많은 비용과 시간이 소요되는 문제가 있다. These sulfur can not be produced naturally in the human body and should be replenished from the outside. The mineral sulfur can not be used for food because of the heavy metal component and toxicity mixed with it. Therefore, most of the animals (such as dung, (Ginger, duck meat, onion, onion, mustard, leek, ginseng, garlic, etc.). However, the amount of sulfur present in the plant is very small, most of which is difficult to be absorbed in the inorganic sulfur state, and it takes a lot of time and cost to ingest the sulfur present in the animal.
이에 종래 당업계에서는 유황의 효능을 그대로 유지하면서 독성을 감소시킨 법제유황을 사료 첨가제로 사용하려는 시도가 이루어졌다. 하지만, 상기 법제유황은 유황의 독성감소 효율이 매우 미약하여 가축의 사료로 이용할 경우 가축의 사멸률이 30% 이상 나타나 제공량에 제한을 두어야 하며, 섭취한 유황이 2시간 이내에 배설물과 함께 배설되어 잔류효과가 적어지는 등의 문제가 있다. 따라서 유황의 독성을 용이하게 제거하는 방법과 더불어 상기 방법에 따라 제조된 유황이 함유된 사료를 제조하려는 시도가 다양하게 이루어지고 있다.In the past, attempts have been made in the art to use legal sulfur as a feed additive in which the toxicity is reduced while maintaining the sulfur efficacy. However, the above-mentioned sulfur is not effective in decreasing the toxicity of sulfur. Therefore, when the animal is used as a feed for livestock, the mortality rate of the livestock should be 30% or more, and the amount of sulfur to be supplied should be limited. There is a problem that the effect is reduced. Therefore, in addition to a method of easily eliminating the toxicity of sulfur, various attempts have been made to produce feeds containing sulfur prepared according to the above method.
이와 관련하여, 대한민국 공개특허 제2010-0019914호에는 콩가루에 함유된 단백질에 바실러스 균주를 접종시켜 1차 발효시킨 후 유황을 첨가하여 다시 2차 발효시킨 다음 액상녹조식물을 첨가하고 가열하는 단계를 통해 독성이 제거된 유황이 함유된 가축용 사료를 제조하는 방법에 대해 개시되어 있어며, 대한민국 등록특허 10-1334157호에는 유산균 및 효모로 이루어진 혼합 미생물 배양액에 독성유황 분말을 첨가한 후 2단계에 걸쳐 발효분해한 후 건조시켜 제독된 유황발효분말을 함유하는 사료 조성물에 대해 개시되어 있다. 그러나, 상기 방법은 단순히 발효 미생물만을 사용하므로 유황의 독성을 제거하는데 다소 많은 시간이 소요되며, 발효 미생물의 종류에 따라 발효시간이 달라져 자동화 공정에 따른 사료의 대량생산이 이루어질 수 없는 문제가 있다. 또한, 가축에 급여하는 경우 증체량, 유량 등은 되나 본 발명에서와 같이 간손상 효소, 콜레스테롤 및 지방 함량을 감소시키는 효과에 대해서는 개시하고 있지 않다. Korean Patent Laid-Open Publication No. 2010-0019914 discloses a method for producing soybean protein by inoculating a protein contained in soybean flour into a primary fermented product, adding sulfur to the fermented product, Korean Patent Registration No. 10-1334157 discloses a method for producing a feed for livestock containing sulfur eliminated with toxicity, wherein a toxic sulfur powder is added to a mixed microbial culture comprising lactic acid bacteria and yeast, Fermented and decomposed, followed by drying, to prepare a feed composition containing the decolorized sulfur fermentation powder. However, since the above method uses only fermenting microorganisms, it takes a long time to remove the toxicity of sulfur, and the fermentation time varies depending on the type of the fermenting microorganism, so that there is a problem that mass production of the feed can not be achieved through the automation process. In addition, when feeding to livestock, weight gain, flow rate and the like are mentioned, but the effect of decreasing the liver damage enzyme, cholesterol and fat content as in the present invention is not disclosed.
이에 본 발명에서는 상기 문제점들을 해결하면서 유황의 독성이 없어 가축의 성장과 사료 이용율을 향상시킬 수 있는 발효사료를 제조하고자 노력하던 중, 유황에 해독효능이 있는 광물 및 약재 추출물과 락토바실러스 카제이(Lactobacillus casei) 및 아세토박터 트로피칼리스(Acetobacter tropicalis)를 사용하여 제조된 액상발효유황과 곡류부산물을 혼합한 후 효모 배양액을 접종하여 발효사료를 제조하는 경우 별도의 기계처리 없이 유황 내 독성물질을 제거할 수 있으며, 가축에 급여하는 경우 액상발효유황에 의해 세균 또는 병원균에 대한 저항력이 향상되어 가축의 폐사율이 감소되고, 증체량 및 사료 요구율이 향상되며, 간손상 효소, 콜레스테롤 및 지방 함량을 감소시킬 수 있음을 확인하고, 본 발명을 완성하게 되었다. Accordingly, the present invention solves the above problems and attempts to produce a fermented feed that can improve the growth of livestock and the feed utilization without sulfur toxicity. Among the minerals and herbal extracts and lactobacillus casei Lactobacillus casei ) and Acetobacter tropicalis , and fermented feedstuffs prepared by inoculating yeast culture liquid after mixing the fermented liquid fermented by-products with cereal by-products, When fed to livestock, liquid fermentation sulfur improves resistance to bacteria or pathogens, resulting in reduced livestock mortality, improved weight gain and feed conversion rate, and reduced liver damage enzymes, cholesterol and fat content. And the present invention was completed.
본 발명의 하나의 목적은 유황의 독성이 없으면서 가축의 성장과 사료 이용율을 향상시킬 수 있는 가축용 발효사료를 제조하는 방법을 제공하는데 있다. It is an object of the present invention to provide a method for producing a fermented feed for livestock which can improve the growth and feed utilization of livestock without toxicity of sulfur.
본 발명의 다른 하나의 목적은 상기 제조방법에 따라 제조된 가축용 발효사료를 제공하는데 있다. It is another object of the present invention to provide a fermented feed for livestock produced according to the above production method.
하나의 양태로서, 본 발명은 하기 단계를 포함하는 것을 특징으로 하는 가축용 발효사료의 제조방법을 제공한다.:In one aspect, the present invention provides a method of preparing a fermented feed for livestock, comprising the steps of:
a) 유황, 운모 및 셀레늄을 물과 두충, 감초 및 회향을 포함하는 약재 추출물에 첨가하여 혼합하는 단계; a) adding sulfur, mica and selenium to the herbal extract, including water, mucilage, licorice and fennel, and mixing;
b) 상기 a) 단계의 혼합물을 교반하면서 저온용융하는 단계;b) low-temperature melting the mixture of step a) with stirring;
c) 상기 b) 단계의 용융물에 당밀, 포도당, 나트륨 및 물을 혼합하여 가열하는 단계; c) mixing and heating molasses, glucose, sodium and water to the melt of step b);
d) 상기 c) 단계의 가열물에 발효균주를 접종한 후 발효시켜 액상발효유황을 제조하는 단계; 및 d) inoculating a fermentation strain into the heated product of step c) and fermenting the fermented product to prepare a liquid fermented sulfur; And
e) 상기 d) 단계의 액상발효유황과 곡류부산물을 혼합한 후 효모 배양액을 접종하여 발효시키는 단계.e) mixing the liquid fermented sulfur of step d) with cereal by-products, and inoculating and fermenting yeast culture liquid.
본 발명에 따른 가축용 발효사료의 제조방법을 각 단계에 따라 구체적으로 설명하면 다음과 같다.The method for producing the fermented feed for livestock according to the present invention will be described in detail with reference to the following respective steps.
a) 유황, 운모 및 셀레늄을 물과 두충, 감초 및 회향을 포함하는 약재 추출물에 첨가하여 혼합하는 단계이다. a) adding sulfur, mica and selenium to the herbal extracts, including water, mites, licorice and fennel, and mixing them.
구체적으로, 본 발명의 a) 단계는 유황, 운모 및 셀레늄을 물에 첨가하여 균질화한 다음, 두충, 감초 및 회향을 포함하는 약재 추출물을 추가로 첨가하여 혼합하는 단계이다.Specifically, the step a) of the present invention is a step of adding sulfuric acid, mica and selenium to water to homogenize them, and then adding and mixing the herbal extracts including moss, licorice, and fennel.
본 발명에 있어서, 상기 유황은 광물성 유황을 흐르는 물에 담가 솔로 문질러 이물질을 제거한 후 건조하고 600 내지 800 메쉬로 분쇄한 분말을 사용한다.In the present invention, the sulfur is soaked in water flowing through mineral sulfur, and is then rinsed with a brush to remove impurities, followed by drying and pulverization with 600 to 800 mesh.
본 발명에 있어서, 상기 운모와 셀레늄은 유황 내 중금속 등 독성물질과 결합하여 독성물질의 활성을 억제시키기 위해 사용되는 것으로서, 유황 100 중량부를 기준으로 하여 각각 45 내지 55 중량부, 바람직하게는 48 내지 52 중량부의 양으로 사용하는 것이 바람직하다. 상기 운모 또는 셀레늄을 45 중량부 미만으로 사용하는 경우 운모 또는 셀레늄과 결합하지 못한 독성물질의 함량이 많아 본 발명에서 목적하는 발효사료를 제조할 수 있으며, 운모 또는 셀레늄을 55 중량부를 초과하여 사용하는 경우 유황 내 독성물질의 활성을 억제되나 운모 또는 셀레늄의 독성에 의해 환경파괴, 질병 유발 등의 2차적인 문제가 발생할 우려가 있다. In the present invention, the mica and selenium are used to inhibit the activity of a toxic substance by binding to a toxic substance such as heavy metals in sulfur. The mica and selenium are contained in an amount of 45 to 55 parts by weight, preferably 48 to 55 parts by weight, It is preferably used in an amount of 52 parts by weight. When the above-mentioned mica or selenium is used in an amount of less than 45 parts by weight, the content of toxic substances which can not be combined with mica or selenium is large, so that the fermented feeds of the present invention can be produced. When mica or selenium is used in an amount exceeding 55 parts by weight The activity of the sulfur poisonous substance is inhibited, but the secondary problem such as environmental destruction and disease induction may occur due to toxicity of mica or selenium.
이때, 상기 운모 또는 셀레늄은 흐르는 물에 담가 솔로 문질러 이물질을 제거한 후 건조하고 600 내지 800 메쉬로 분쇄한 분말을 사용한다. At this time, the mica or selenium is soaked in flowing water, rubbed with a brush, and then dried to remove foreign matters, and the powder is pulverized to 600 to 800 mesh.
본 발명에 있어서, 상기 두충, 감초 및 회향을 포함하는 약재 추출물은 운모와 셀레늄에 의해 제거되지 못한 독성물질을 제거하는 동시에 사료의 저장성을 향상시키기 위해 사용되는 것으로서, 두충, 감초 및 회향을 혼합하고 당업계에 공지된 통상적인 용매를 사용하여 추출한 다음, 추출액의 총 중량을 기준으로 추출액의 용매를 48 내지 52중량%로 농축된 것을 사용하는 것이 좋다. 이때, 상기 두충, 감초 및 회향은 용매 100 중량부에 대하여 각각 1 내지 3 중량부, 1 내지 3 중량부 및 0.5 내지 1.5 중량부를 사용하는 것이 좋다.In the present invention, the medicinal plant extract containing the mites, licorice, and fennel is used for removing toxic substances not removed by mica and selenium and at the same time improving the storage stability of the feed. It is preferable to use a solvent which has been extracted with a conventional solvent known in the art and then concentrated to a concentration of 48 to 52% by weight of the solvent of the extract based on the total weight of the extract. Preferably, 1 to 3 parts by weight, 1 to 3 parts by weight, and 0.5 to 1.5 parts by weight of the above-mentioned mites, licorice and fennel are used relative to 100 parts by weight of the solvent.
상기 용매는 예를 들어, (a) 물, (b) 탄소수 1-4의 무수 또는 함수 저급 알코올(메탄올, 에탄올, 프로판올, 부탄올 등), (c) 상기 저급 알코올과 물과의 혼합용매, (d) 아세톤, (e) 에틸 아세테이트, (f) 클로로포름 또는 (g) 1,3-부틸렌글리콜을 추출 용매로 하여 수득할 수 있다. 바람직하게는, 물, 메탄올 또는 에탄올을 이용하여 추출하는 것이 좋다. 추출하는 유기용매에 따라 추출물의 유효성분의 추출정도와 손실정도가 차이가 날 수 있으므로, 알맞은 유기용매를 선택하여 사용하도록 한다. 상기 추출 방법은 특별히 제한되지 않고, 예를 들어 열수 추출, 냉침 추출, 초음파 추출, 환류 냉각 추출 등이 있다.The solvent may be, for example, (a) water, (b) an anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohol and water, d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol as extraction solvents. Preferably, extraction is carried out using water, methanol or ethanol. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the extract may differ. Therefore, an appropriate organic solvent should be selected and used. The extraction method is not particularly limited, and examples thereof include hot water extraction, cold extraction, ultrasonic extraction, and reflux cooling extraction.
상기 농축은 추출물 중의 용매를 제거하여 추출물의 농도를 높이는 방법이라면 어느 것이나 사용 가능하며, 예를 들어 가열 농축, 감압 농축, 통풍 농축, 냉동농축, 분무 농축 등이 있다. The concentration may be any method for removing the solvent in the extract to increase the concentration of the extract, for example, heating, concentration under reduced pressure, concentration by gust, freezing concentration, and spray concentration.
상기 두충, 감초 및 회향은 당업자에 의해 용이하게 입수 가능한 것으로서, 상업적으로 판매되는 상품 자체를 사용하거나 일정크기로 절단 또는 절단 후 볶은 것을 사용할 수 있으며, 바람직하게는 절단 후 볶은 것을 사용하는 것이 좋다. 이는 각 재료의 특성에 따라 각각의 성분이 용매에 잘 침출되게 하기 위함이다.The fungus, licorice, and fennel are readily available to those skilled in the art. Commercially available products may be used or they may be cut or cut into a certain size and roasted, preferably roasted after cutting. This is to allow each component to leach well into the solvent depending on the properties of each material.
본 발명에 있어서, 상기 두충, 감초 및 회향을 포함하는 약재 추출물은 유황 100 중량부를 기준으로 하여 45 내지 55 중량부, 바람직하게는 48 내지 52 중량부의 양으로 사용하는 것이 바람직하다.In the present invention, it is preferable that the medicinal plant extract containing the mites, licorice and fennel is used in an amount of 45 to 55 parts by weight, preferably 48 to 52 parts by weight, based on 100 parts by weight of sulfur.
본 발명의 액상발효유황을 제조하는데 사용되는 약재의 효능을 살펴보면 다음과 같다. The efficacy of the medicinal materials used for producing the liquid fermentation sulfur of the present invention will be described below.
두충은 두충나무(Eucommia ulmoides Oliver)의 나무껍질을 말린 약재로, 구타페르카(gutta-percha), 배당체, 알카로이드, 펙틴, 수지, 유기산, 케스트, 비타민 C, 클로로겐산 등이 함유되어 있어 혈압강하, 항노화, 콜레스테롤 강하, 항염, 면역 증대, 체내 정화작용, 항균작용 및 항알레르기 등에 효과가 있다고 알려져 있다. Eucommia ulmoides Oliver is a dried bark and contains gutta-percha, glycosides, alkaloids, pectin, resin, organic acid, cast, vitamin C and chlorogenic acid. Anti-aging, cholesterol lowering, anti-inflammation, immunity enhancement, cleansing effect in the body, antibacterial activity and antiallergic.
감초(Glycyrrhiza uralensis Fischer)는 쌍떡잎식물 장미목 콩과에 속하는 식물로, 글리시리진(glycyrrhizin), 리퀴리티게닌(liquiritigenin), 리퀴리틴(lliquiritin) 등의 플라보노이드(flavonoid)를 함유하고 있어 단맛을 내며, 항균, 항염증, 항암, 및 약물과 식물 등에 의한 중독을 해독하는 등의 효과가 있다고 알려져 있다.Licorice ( Glycyrrhiza uralensis Fischer) is a plant belonging to the family Rosaceae leguminosae, and contains flavonoids such as glycyrrhizin, liquiritigenin and lliquiritin, Antimicrobial, anti-inflammatory, anti-cancer, and poisoning by drugs and plants.
회향은 회향나무(Foeniculum vulgare Miller)의 성숙한 과실을 건조한 것으로, 아네톨(anethole), 펜촌(fenchone), 피넨(piene), 리모네(limone) 등으로 이루어진 정유성분을 함유하고 있어 기운순환을 촉진하고, 위기능을 강화하며, 유해물질 배출 및 항균 등에 효과가 있다고 알려져 있다. Fennel is a dried fruit of the mature fruit of Feniculum vulgare Miller and contains essential oil components such as anethole, fenchone, piene and limone, And strengthen the above functions, and is said to be effective in the emission of harmful substances and antibacterial.
b) 상기 a) 단계의 혼합물을 교반하면서 저온용융하는 단계이다. and b) low-temperature melting the mixture of step a) with stirring.
구체적으로, 본 발명의 b) 단계는 상기 a) 단계의 혼합물을 38 내지 45℃의 온도에서 45 내지 60일 동안 교반하여 이루어지는 것을 특징으로 한다. 상기 교반이 38℃ 미만이거나 45일 미만으로 이루어지는 경우 혼합물의 용융이 이루어지지 않아 액상상태의 혼합물을 얻기 어려운 문제가 있으며, 교반이 45℃를 초과하거나 50일을 초과하여 이루어지는 경우 별도의 가열장치가 필요하므로 발효사료를 제조하는 비용이 많이 소요되어 비경제적이다. Specifically, step (b) of the present invention is characterized in that the mixture of step (a) is stirred at a temperature of 38 to 45 DEG C for 45 to 60 days. If the stirring is less than 38 ° C or less than 45 days, the mixture is not melted and it is difficult to obtain a liquid mixture. If stirring is performed at more than 45 ° C or more than 50 days, a separate heating device It is costly to manufacture a fermented feed, which is uneconomical.
이때, 용융은 독물 취급이 용이한 밀폐된 용기, 예를 들어 알루미늄 용기, 스테인레스 용기 등에서 이루어지는 것이 좋다. 여기서, 상기 밀폐된 공간은 외부의 공가나 이물질로부터 차단된 공간을 의미한다. At this time, it is preferable that the melting is performed in a closed container which is easy to handle poison, for example, an aluminum container, a stainless container, or the like. Here, the enclosed space means a space blocked from external air or foreign matter.
이러한 저온용융공정은 유황을 액상의 상태로 변화시킴과 동시에 약재에 함유되어 있는 미생물이 생장하기 적합한 환경을 만들어주어 별도의 미생물 발효공정 없이도 약재로부터 생리활성물질을 얻을 수 있는 이점이 있다.The low-temperature melting process has an advantage that the sulfur can be changed into a liquid state and at the same time, an environment suitable for the growth of the microorganisms contained in the medicinal material can be produced, so that a physiologically active substance can be obtained from the medicinal material without a separate microbial fermentation process.
c) 상기 b) 단계의 용융물에 당밀, 포도당, 나트륨 및 물을 혼합하여 가열하는 단계이다.c) mixing molasses, glucose, sodium and water to the melt of step b) and heating.
구체적으로, 상기 c) 단계는 상기 b) 단계의 용융물에 용융물 100 중량부를 기준으로 하여 당밀 90 내지 110 중량부, 포도당 40 내지 60 중량부, 나트륨 20 내지 30 중량부 및 물 4,000 내지 6,000 중량부를 혼합하여 90 내지 110℃의 온도로 60 내지 70분 동안 가열하여 이루어지는 것을 특징으로 한다. Specifically, in step c), 90 to 110 parts by weight of molasses, 40 to 60 parts by weight of glucose, 20 to 30 parts by weight of sodium and 4,000 to 6,000 parts by weight of water are mixed with the melt of step b) Followed by heating at a temperature of 90 to 110 DEG C for 60 to 70 minutes.
이러한 가열공정은 상기 a) 단계에서 운모 또는 셀레늄과 결합된 독성물질을 휘발시켜 유황이 가지고 있는 독성물질을 추가적으로 제거시킴과 동시에 당밀 또는 포도당 내에 존재하는 세균이나 병원균을 사멸시킬 수 있으므로 별도의 항생제를 사용하지 않고도 2차적인 오염을 방지할 수 있는 이점이 있다.This heating step volatilizes the mucous or selenium-bonded toxic substances in step a) to remove the toxic substances contained in the sulfur and can kill germs or pathogens present in the molasses or glucose. Therefore, a separate antibiotic There is an advantage that second pollution can be prevented without using it.
d) 상기 c) 단계의 가열물에 발효균주를 접종한 후 발효시켜 액상발효유황을 제조하는 단계이다.d) Inoculating a fermentation strain into the heated product of step c), followed by fermentation to produce liquid fermented sulfur.
본 발명에 있어서, 상기 발효균주는 락토바실러스 카제이(Lactobacillus casei) 및 아세토박터 트로피칼리스(Acetobacter tropicalis)가 1:1의 중량비로 혼합된 혼합균주인 것을 특징으로 한다. In the present invention, the strain is characterized in that the strain is a mixed strain in which Lactobacillus casei and Acetobacter tropicalis are mixed at a weight ratio of 1: 1.
상기 락토바실러스 카제이(Lactobacillus casei)는 당류를 발효하여 젖산과 함께 초산, 프로피온산, 에틸 알콜, 탄산가스 등을 생성하는 발효 유산균으로, 다른 유산균에 비하여 성장속도가 액상유황의 발효기간을 최소화할 수 있다. The Lactobacillus casei is a fermented lactic acid bacterium that fermentes a saccharide to produce acetic acid, propionic acid, ethyl alcohol, carbonic acid gas and the like together with lactic acid. The growth rate of the lactic acid bacteria is minimized when the fermentation period of liquid sulfur is minimized have.
상기 아세토박터 트로피칼리스(Acetobacter tropicalis)는 상기 b) 단계의 용융된 유황에서 분리된 초산균으로, 아세토박터 트로피칼리스(Acetobacter tropicalis) NRIC 0312T(AB032354)와 90% 이상, 바람직하게는 95% 이상, 보다 바람직하게는 98% 이상 상동성을 가지는 것을 특징으로 하며, 당밀(molasses) 또는 포도당(glucose)으로부터 초산을 생성하여 다른 유산균에 비하여 액상유황의 pH를 저하시키는 효능이 뛰어나 부패성 세균의 증식을 억제할 수 있다. The acetonitrile bakteo trophy faecalis (Acetobacter tropicalis) is the b) with the separated chosangyun in the molten sulfur phase, acetonitrile bakteo trophy faecalis (Acetobacter tropicalis) NRIC 0312 T ( AB032354) with more than 90%, preferably at least 95%, More preferably 98% or more homologous. It is excellent in the effect of lowering the pH of liquid sulfur as compared with other lactic acid bacteria by producing acetic acid from molasses or glucose and inhibiting the proliferation of perishable bacteria can do.
본 발명의 락토바실러스 카제이(Lactobacillus casei) 또는 아세토박터 트로피칼리스(Acetobacter tropicalis)는 균주 자체 또는 계대 배양용 배지에 각각의 균주를 접종하여 균주가 증식된 배양액을 사용할 수 있으며, 바람직하게는 계대 배양용 배지에 각각의 균주를 접종하여 균주가 증식된 배양액이다. 하나의 예로서, 상기 락토바실러스 카제이(Lactobacillus casei) 또는 아세토박터 트로피칼리스(Acetobacter tropicalis)는 락토바실러스 카제이(Lactobacillus casei) UPRO 02(KCCM 10937P) 또는 아세토박터 트로피칼리스(Acetobacter tropicalis) NRIC 0312T(AB032354)와 99% 상동성을 가지는 균주를 고압 멸균된 MRS 액체배지(MRS broth; peptone 10g/L, beef extract 10g/L, yeast extract 5g/L, dextrose 10g/L, diammonium-citrate 3g/L, sodium acetate 5g/L, tween 80 1ml, K2HPO4 2g/L, MgSO4ㅇ7H2O 0.2g/L, MnSO4ㅇ7H2O 0.2g/L, pH 6.2-6.6) 10㎖에 접종하여 20 내지 40℃의 온도에서 48시간 동안 교반 배양한 것이다.The Lactobacillus casei or Acetobacter tropicalis of the present invention can be obtained by inoculating each strain into a culture medium for the strain itself or a subculture culture and using the culture medium in which the strain has been grown, And the strain is inoculated with each of the strains in the culture medium. As one example, the Lactobacillus casei or Acetobacter tropicalis may be selected from the group consisting of Lactobacillus casei UPRO 02 (KCCM 10937P) or Acetobacter tropicalis NRIC 0312 T (MRS broth: 10 g / L of peptone, 10 g / L of beef extract, 5 g / L of yeast extract, 10 g / L of dextrose, 3 g / L of diammonium-citrate in a high pressure sterilized MRS broth , sodium acetate 5 g / L, tween 80 1 ml, K 2 HPO 4 2 g / L, MgSO 4 ㅇ 7H 2 O 0.2 g / L, MnSO 4 ㅇ 7H 2 O 0.2 g / L, pH 6.2-6.6) And cultured at a temperature of 20 to 40 DEG C for 48 hours with stirring.
본 발명에 있어서, 상기 발효는 상기 c) 단계의 가열물에 가열물과 동일한 중량의 상기 발효균주를 접종한 후 28 내지 40℃, 바람직하게는 30 내지 40℃, 보다 바람직하게는 35 내지 40℃의 온도에서 65 내지 80시간, 바람직하게는 68 내지 75시간 동안 이루어지는 것이 바람직하다. In the present invention, the fermentation is carried out by inoculating the fermentation strain of the same weight as the heated product into the heated product of the step c), and then heating the fermentation strain at 28 to 40 캜, preferably 30 to 40 캜, more preferably 35 to 40 캜 Deg.] C for 65 to 80 hours, preferably 68 to 75 hours.
상기한 바와 같이 제조된 액상발효유황은 유황의 독성물질이 완전히 제거되었으며, 세균 또는 병원균의 생장을 억제하는 우수한 항균활성을 나타낸다. The liquid fermented sulfur produced as described above has an excellent antimicrobial activity which completely removes toxic substances of sulfur and inhibits the growth of bacteria or pathogens.
e) 상기 d) 단계의 액상발효유황과 곡류부산물을 혼합한 후 효모 배양액을 접종하여 발효시키는 단계이다.e) mixing the liquid fermented sulfur in step d) with the by-product of the cereal, and then inoculating the fermented yeast culture solution.
구체적으로, 본 발명의 e) 단계는 상기 d) 단계의 액상발효유황과 곡류부산물을 1:4 내지 6의 중량비, 바람직하게는 1:5의 중량비로 혼합한 후 효모 배양액을 접종하여 발효시켜 이루어지는데, 이러한 공정은 곡류부산물에 함유된 섬유질 및 난분해성 단백질을 분해하여 가축의 사료 이용율을 향상시킬 수 있다.Specifically, in step e) of the present invention, the liquid fermentation sulfur and the by-product of step d) are mixed at a weight ratio of 1: 4 to 6, preferably 1: 5, and the yeast culture solution is inoculated and fermented This process can improve the feed utilization of livestock by degrading the fibrous and degradable proteins contained in cereal by-products.
본 발명에 있어서, 상기 효모 배양액은 곡류부산물의 섬유질을 분해하는 미생물로서, 액상발효유황과 곡류부산물을 혼합한 혼합물 100 중량부에 대하여 0.05 내지 0.2 중량부, 바람직하게는 0.1 중량부를 사용하는 것이 바람직하다. 상기 효모 배양액을 0.05 중량부 미만으로 사용하는 경우 섬유질의 분해가 이루어지지 않아 가축에 제공하는 경우 소화율이 현저히 저하되며, 효모 배양액을 0.2 중량부를 초과하여 사용하는 경우 효모에 의해 부패가 빠르게 일어나 저장기간이 감소되는 문제가 있다.In the present invention, the yeast culture broth is a microorganism that degrades the fibers of cereal by-products, and it is preferable to use 0.05 to 0.2 parts by weight, preferably 0.1 parts by weight, based on 100 parts by weight of the mixture of liquid fermented sulfur and cereal by-products Do. When the yeast culture solution is used in an amount of less than 0.05 part by weight, decomposition of the fiber is not performed, and digestibility is significantly reduced when the yeast culture solution is added to the livestock. When the yeast culture solution is used in an amount exceeding 0.2 part by weight, Is reduced.
본 발명에 있어서, 상기 곡류부산물은 가축의 사용에 사용되는 것이라면 어느 것이나 사용 가능하며, 예를 들어 보리, 라이그라스, 밀, 호밀, 귀리, 옥수수, 벼, 콩, 완두, 수수, 라이밀, 두부콩 및 쌀 등으로 이루어진 군으로부터 선택된 1종 또는 2종 이상의 혼합물로 조성된 것일 수 있다. In the present invention, the cereal by-products may be any of those used for the use of livestock such as barley, rice, wheat, rye, oats, corn, rice, beans, peas, sorghum, Soybean, rice, and the like.
상기 가축은 본 발명의 발효사료를 이용하여 사육가능한 동물을 말하며, 예를 들어, 돼지, 염소, 말, 젖소, 기니아피그 등과 같은 포유동물, 닭, 오리, 벌새 등과 같은 조류, 아로와나, 전갈, 타란튤라, 비어드, 모니터, 게코 등과 같은 육식곤충, 뱀, 이구아니, 도마뱀, 카멜레온 등과 같은 파충류, 개구리, 두꺼비, 도룡뇽 등과 양서류 또는 홍어, 연어, 돔, 대구, 붕어 등과 같은 어류를 들 수 있다.The animal is an animal capable of breeding using the fermented feed according to the present invention. Examples of the animal include rabbits such as pigs, goats, horses, cows, guinea pigs, etc., birds such as chickens, ducks, hummingbirds, , Carnivorous insects such as beards, monitors, and geckos, reptiles such as snakes, lizards, lizards, and chameleons, frogs, toads, salamanders, and amphibians or fish such as skates, salmon, dome, codfish and crucian carp.
다른 하나의 양태로서, 본 발명은 상기 방법에 따라 제조된 축산용 발효사료를 제공한다.In another aspect, the present invention provides a fermented feed for livestock produced according to the above method.
본 발명에 있어서, 상기 축산용 발효사료는 가축에 급여하는 경우 액상발효유황에 의해 세균 또는 병원균에 대한 저항력이 향상되어 가축의 폐사율이 감소되고, 증체량 및 사료 요구율이 향상되며, 간손상 효소, 콜레스테롤 및 지방 함량을 감소시키는 효과를 나타낸다. 또한, 가축의 성장을 촉진시켜 단위 개체당 생산비를 절감시킬 수 있으며, 그 육질의 맛도 좋게 할 수 있는 이점이 있다. In the present invention, when the fermented feed for livestock is fed to livestock, the resistance against bacteria or pathogens is enhanced by the liquid fermentation sulfur, so that the mortality rate of the livestock is reduced, the amount of feed and the feed rate are improved, and the liver damage enzyme, cholesterol And an effect of reducing the fat content. In addition, the growth of livestock can be promoted to reduce the production cost per unit, and the meat taste can be improved.
본 발명에 따라 제조된 축산용 발효사료는 가축에 급여하는 경우 액상발효유황에 의해 가축의 폐사율을 감소시키고, 가축에 급여하는 경우 증체량 및 사료 요구율이 향상되며, 간손상 효소, 콜레스테롤 및 지방 함량을 감소시키는 효과를 나타낸다. 또한, 가축의 성장을 촉진시켜 단위 개체당 생산비를 절감시킬 수 있으며, 그 육질의 맛도 좋게 할 수 있는 이점이 있다. The fermented feed for livestock produced according to the present invention reduces the mortality of livestock by liquid fermented sulfur when fed to livestock and improves the amount of feed and feed ratio when fed to livestock and improves the liver damage enzyme, . In addition, the growth of livestock can be promoted to reduce the production cost per unit, and the meat taste can be improved.
도 1은 발효미생물의 종류에 따른 발효기간 동안 액상유황의 pH를 측정한 그래프이다.1 is a graph showing the pH of liquid sulfur measured during fermentation according to the kind of fermenting microorganism.
이하, 실시예 등을 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예 등은 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예 등에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail by way of examples and the like. It will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples and the like according to the gist of the present invention. will be.
실시예 1: 액상유황 제조Example 1: Production of liquid sulfur
1-1. 혼합단계1-1. Mixing step
분재로 된 황색의 유황 1000kg, 운모 500kg, 셀레늄 500g 및 물 1500L을 혼합한 후, 두충 20kg, 감초 20kg 및 회향 10kg을 물 1000L에 넣고 끓인 추출물을 500L로 농축하여 혼합하였다.1000 kg of yellow sulfur as a bonsai, 500 kg of mica, 500 g of selenium and 1500 L of water were mixed, and then 20 kg of mulberry, 20 kg of licorice and 10 kg of fennel were added to 1000 L of water and the boiled extract was concentrated to 500 L and mixed.
1-2. 용융단계 1-2. Melting step
상기 1-2의 혼합물을 알루미늄 또는 스테인레스 용기에 넣고 밀폐시킨 후 40℃에서 50일 동안 교반하면서 용융하였다. The mixture of 1-2 was put in an aluminum or stainless steel container, sealed, and melted at 40 DEG C for 50 days with stirring.
1-3. 액상유황 제조단계1-3. Liquid sulfur production step
상기 1-2에서 용융시킨 유황과 당밀, 포도당, 나트륨, 물을 1 : 1 : 0.5: 0.25 : 50 비율로 혼합한 혼합물을 스테인레스 용기에 넣고 공기차단 후, 100℃에서 1시간 동안 가열하여 액상유황을 제조하였다. A mixture of sulfur, molasses, glucose, sodium, and water in a molar ratio of 1: 1: 0.5: 0.25: 50 was added to a stainless steel vessel, and air was blocked. The mixture was heated at 100 ° C. for 1 hour, .
실시예 2: 발효 미생물 종류 선별 실험Example 2: Selection of fermenting microorganism species
액상유황의 발효기간을 단축시킬 수 있는 미생물을 선별하기 위해, 상기 실시예 1에서 제조된 전 배양물 100mL에 락토바실러스 루테리(Lactobacillus reuteri), 페디오코커스 롤리(Pediococcus lolli), 락토바실러스 플란타륨(Lactobacillus plantarum), 락토바실러스 카제이(Lactobacillus casei) 또는 아세토박터 트로피칼리스(Acetobacter tropicalis) 배양액 5mL을 접종한 후 37℃에서 20일 동안 배양하며, 2일 간격으로 pH 측정기(M503P meter, wrks, Medifield, MA, USA)를 이용하여 pH를 측정하였다. 이때, 상기 아세토박터 트로피칼리스(Acetobacter tropicalis)는 상기 실시예 1-2의 용융된 유황으로부터 분리된 것으로, 아세토박터 트로피칼리스(Acetobacter tropicalis) NRIC 0312(T)와 99% 상동성을 나타내는 균주이다. 대조군(control)으로는 미생물을 접종하지 않은 액상유황을 사용하였다. 그 결과를 도 1에 나타내었다.To select microorganisms capable of shortening the fermentation period of liquid sulfur, 100 mL of the preculture prepared in Example 1 was added with Lactobacillus reuteri , Pediococcus lolli , Lactobacillus platarium 5 ml of Lactobacillus plantarum , Lactobacillus casei or Acetobacter tropicalis were inoculated and cultured at 37 ° C for 20 days. The cells were cultured at 2-day intervals using a pH meter (M503P meter, wrks, Medifield , MA, USA). At this time, the Acetobacter tropicalis was isolated from the molten sulfur of Example 1-2, and was a strain showing 99% homology with Acetobacter tropicalis NRIC 0312 (T). As a control (control), liquid sulfur not inoculated with microorganisms was used. The results are shown in Fig.
도 1에서 보듯이, 미생물 접종초기에 대조군에 비해 모든 처리군에서 pH가 감소되었으며, 발효 1일째에는 락토바실러스 카제이(Lactobacillus casei)를 접종한 처리군의 pH가 가장 낮게 측정되었으며, 발효 20일째에는 아세토박터 트로피칼리스(Acetobacter tropicalis)를 접축한 처리군의 pH가 가장 낮게 측정됨이 확인되었다.As shown in FIG. 1, the pH of the treated group was lower than that of the control group at the initial stage of microbial inoculation. On the first day of fermentation, the pH of the treated group inoculated with Lactobacillus casei was lowest, It was confirmed that the pH of the group treated with Acetobacter tropicalis was lowest.
이에 본 발명에서는 액상유황을 발효시키는 미생물로 락토바실러스 카제이(Lactobacillus casei)와 아세토박터 트로피칼리스(Acetobacter tropicalis)를 선정하였으며, 이를 1:1의 중량비로 혼합하여 사용하였다. In the present invention, Lactobacillus casei and Acetobacter tropicalis were selected as microorganisms fermenting liquid sulfur, and they were mixed at a weight ratio of 1: 1.
실시예 3: 액상발효유황이 함유된 유황발효사료 제조Example 3: Production of sulfur fermented feed containing liquid fermented sulfur
락토바실러스 카제이 UPRO 02(Lactobacillus casei UPRO, KCCM 10937P)와 아세토박터 트로피칼리스 NRIC 0312T(cetobacter tropicalis NRIC 0312T)와 99% 상동성을 나타내는 균주 배양액을 1:1로 혼합한 미생물을 상기 실시예 1-3의 액상유황과 1:1로 혼합한 다음, 37℃에서 72시간 1차 발효하여 액상발효유황을 제조하였다. 그 다음, 상기 액상발효유황과 곡류부산물을 혼합한 후 효모배양액을 접종하여 38℃에서 72시간 동안 2차 발효하여 유황발효사료를 제조하였다. 이때, 상기 액상발효유황, 곡류부산물 및 효모배양액의 혼합비율은 하기 표 1에 나타내었다.Lactobacillus casei UPRO 02 (Lactobacillus casei UPRO, KCCM 10937P) and acetonitrile bakteo trophy faecalis NRIC 0312 T (cetobacter tropicalis NRIC 0312 T) and 99% of a strain culture solution representing Bi 1: a microorganism mixture with 1 Example 1 to 1: 1 liquid sulfur, followed by primary fermentation at 37 ° C for 72 hours to prepare liquid fermented sulfur. Then, the liquid fermented sulfur and the cereal by-products were mixed, and the fermented milk was fermented by inoculating the yeast culture solution at 38 ° C for 72 hours for secondary fermentation. At this time, the mixing ratios of the liquid fermented sulfur, the by-products of the cereals, and the yeast culture liquid are shown in Table 1 below.
시험예 1: 본 발명에 따라 제조된 유황발효사료의 성분 분석Test Example 1: Analysis of components of fermented milk produced according to the present invention
상기 실시예 3에서 제조한 유황발효사료의 조섬유는 AOAC(2000)방법에 따라 수분은 상압가열건조법(125℃, 3시간), 조단백질은 kjeldahl 질소정량법(Nㅧ6.25), 조회분은 직접 회화법으로 분석하였다. 또한, 아미노산 함량은 아미노산 전용 분석기(Sykam amino acid analyer S433, 독일)를 이용하여 Ninhydrin방법으로 분석하였으며, 효모의 균수는 YGC agar에 배양하여 생성된 콜로니를 계수하여 분석하였다. 그 결과를 표 2에 나타내었다. According to the AOAC (2000) method, the moisture of the crude fermented feed prepared in Example 3 was subjected to the atmospheric pressure heat drying method (125 ° C., 3 hours), the crude protein was measured by the kjeldahl nitrogen determination method (N ㅧ 6.25) Respectively. Amino acid content was analyzed by Ninhydrin method using amino acid analyzer (Sykam amino acid analyzer S433, Germany). The number of yeast cells was counted by counting colonies formed by culturing on YGC agar. The results are shown in Table 2.
시험예 2: 본 발명의 유황발효사료 급여에 따른 육계의 이화학적 특성분석Test Example 2: Analysis of physicochemical properties of broiler chickens fed with sulfur fermented feed of the present invention
2-1. 공시동물 및 시험설계2-1. Disclosure Animal and Test Design
공시동물은 담양에 위치한 부화장에서 생산된 1일령 닭(Ross broiler) 48,0121마리를 공시하였다. 시험설계는 대조군 25,986 마리, 유황발효사료 급여군 22,035 마리로 나누어 시판용 배합사료(CP 18.5%, ME 3,250 Kcal/kg)와 유황발효사료가 0.2% 첨가된 시판용 배합사료를 2015년 5월 19일에 개시하여 2015년 6월22일까지 총 34일 동안 급여하였다.The animals were 48,0121 Ross broiler (1 day old) produced in the hatchery in Damyang. The experimental design was carried out on May 19, 2015, with 25.986 control and 25.01 kcal fermented diets fed with commercial mixed feed (CP 18.5%, ME 3,250 Kcal / kg) and 0.2% sulfur fermented feed And paid for a total of 34 days until June 22, 2015.
2-2. 유황발효사료의 급여에 따른 육계의 증체량, 사료섭취량, 사료요구율 및 폐사율 측정2-2. Measurement of body weight gain, feed intake, feed conversion rate and mortality of broiler chicks fed with fermented milk
유황발효사료의 급여에 따른 육계의 증체량은 시험 개시시와 종료시에 체중을 측정하여 종료시 체중에서 개시시 체중을 감하여 구하였다. 유황발효사료의 급여에 따른 사료섭취량은 시험 전 기간 동안의 사료 급여량에서 잔량을 공제하여 섭취량을 구하였으며, 사료요구율은 사료 섭취량을 증체량으로 나누어서 구하였다. 유황발효사료의 급여에 따른 폐사율은 폐사하는 육계의 수를 매일 측정하였으며, 시작시 공시계수에서 폐사한 육계의 수로 나누어 백분율로 나타내었다. 그 결과를 표 3에 나타내었다. The weight gain of broiler chickens fed with sulfur fermented diets was measured by measuring body weight at the beginning and at the end of the test and by subtracting body weight at the start of body weight at the end. The feed intake ratio was calculated by subtracting the residual amount from the feed amount during the period before the test, and the feed conversion ratio was determined by dividing the feed intake by the amount of gain. The mortality rate according to the feeding of sulfur fermented diets was measured daily by the number of dead broiler chicks, divided by the number of broiler chicks in broiler count at the start and expressed as a percentage. The results are shown in Table 3.
상기 표 3에서 보듯이, 유황발효사료 급여에 따른 증체량은 유황발효사료 급여군이 1,775g으로 대조군에 비해 높게 나타났으며, 사료 섭취량 역시 유황발효사료 급여군이 2,898g으로 대조군에 비해 높게 나타났다. 또한, 사료 요구율 및 폐사율은 유황발효사료 급여군이 대조군에 비해 낮게 나타나 유황발효사료 급여는 사료효율 및 생산성 향상에 효과가 있음을 알 수 있었다. As shown in Table 3, the amount of milk fed fermented milk was higher than that of the control group by 1,775 g in the fermented milk feed group, and the feed intake was 2,898 g in the fermented milk feed group, which was higher than that of the control group. Feeding rate and mortality were lower in the fermented milk fed group than in the control group, indicating that feeding of fermented milk was effective in improving feed efficiency and productivity.
2-3. 유황발효사료의 급여에 따른 육계의 분변 미생물 성상 분석2-3. Analysis of fecal microorganism characteristics of broiler chickens fed with sulfur fermented feed
유황발효사료의 급여에 따른 육계의 분변 미생물 성상은 시험 0일, 3주 및 종료 시 처리구별로 분변을 채취한 뒤, 멸균 생리식염수에 현탁하여 균질화시킨 후 10진 희석하여 각 배지에 도말하여 37℃에서 48시간 배양하여 생균수를 측정하였다. 분변 내 미생물 균수측정은 총균수, 살모넬라, 대장균, 유산균 및 효모의 균수를 측정하였다. 총균수 측정은 TSA agar에 배양하여 콜로니를 계수하였다. 살모넬라 측정은 XLT4 agar에 배양하여 검은색을 띄는 콜로니를 계수하였고, 대장균 측정은 Mac conkey agar에 배양하여 붉은색을 띄는 콜로니를 계수하였고, 유산균 측정은 BCP를 첨가한 MRS agar에 배양하여 콜로니 주변에 노란색을 띄는 콜로니를 계수하였고, 효모 측정은 YGC agar에 배양하여 흰색 콜로니를 계수하여 측정하였다. 그 결과를 표 4에 나타내었다.The fecal microorganism of broiler chickens fed with sulfur fermented diets was sampled at the 0th, 3th, and the end of the test, and the feces were collected and sterilized in sterilized physiological saline, homogenized, ≪ / RTI > for 48 hours. The number of microorganisms in the feces was measured by counting the total bacteria, Salmonella, Escherichia coli, lactic acid bacteria and yeast. Total bacterial counts were counted by culturing on TSA agar. Salmonella assay was performed on XLT4 agar and black colonies were counted. Escherichia coli was cultured on Mac conkey agar to count red colonies. Lactic acid bacteria were cultured on MRS agar supplemented with BCP, Yellowish colonies were counted and yeast counts were determined by counting white colonies cultured on YGC agar. The results are shown in Table 4.
(Lactobacilli) Lactobacillus
( Lactobacilli)
(Salmonella sp.) Salmonella
(Salmonella sp.)
(Lactobacilli) Lactobacillus
( Lactobacilli)
(Salmonella sp.) Salmonella
(Salmonella sp.)
(Lactobacilli) Lactobacillus
( Lactobacilli)
(Salmonella sp.) Salmonella
(Salmonella sp.)
도 4에서 보듯이, 유황발효사료 급여에 따른 육계의 분내 유산균의 수는 유황발효사료 급여 14일차에 대조군에서 가장 높게 측정된 반면(P<0.05), 7일 및 34일차에서는 유황발효사료 급여군에서 높은 경향을 보였지만 처리구간 유의차는 없었다. 측정 기간 동안 대조군과 유황발효사료 급여군 간의 유의차가 없었지만 총 균수는 대조군에서 높은 경향을 보였고, 효모는 유황발효사료 급여군에서 높은 경향을 보였다. As shown in FIG. 4, the number of lactic acid bacteria in broiler chicks fed sulfur fermented diets was the highest in the control group at the 14th day of fermented milk fed diet ( P <0.05) , But there was no significant difference in treatment interval. There was no significant difference between the control group and the fermented feed group during the measurement period, but the total number of bacteria was higher in the control group and the yeast was higher in the fermented milk feed group.
대장균과 살모넬라는 대조군과 유황발효사료 급여군 간에 유의차를 보이지 않았으며, 시간이 경과함에 따라 대장균수와 살모넬라 균수가 줄어드는 경향을 보였다. There was no significant difference between coliform and salmonella in the control and sulfur fermented feed groups, and the number of E. coli and Salmonella bacteria tended to decrease with time.
2-4. 유황발효사료의 급여에 따른 육계의 혈액성상2-4. The blood characteristics of broiler chickens fed with sulfur fermented feed
유황발효사료의 급여에 따른 육계의 혈액성상은 시험 종료시 대조군과 유황발효사료 급여군에서 임의로 3마리씩 선발하여 정맥에서 혈액을 2ml 채혈하여 vacutainer에 옮겨 담은 후 5,000rpm으로 30분간 원심 분리하여 얻은 혈청을 분리하였다. 그 다음 자동 혈액분석기(COBAS MIRA plus,ROCHE diagnostics)를 사용하여 혈청 내 간 손상 수치(AST, ALT), 총 콜레스테롤(total cholesterol), 저밀도 콜레스테롤(LDL cholesterol), 고밀도 콜레스테롤(HDL cholesterol) 함량을 측정하였다. 그 결과를 표 5에 나타내었다.At the end of the experiment, 3 rats were randomly selected from the control group and the fermented feed group at the end of the experiment, 2 ml of blood was collected from the venous blood, transferred to a vacutainer, and centrifuged at 5,000 rpm for 30 minutes. Respectively. (AST, ALT), total cholesterol, LDL cholesterol, and HDL cholesterol levels were measured using an automated blood analyzer (COBAS MIRA plus, ROCHE diagnostics) Respectively. The results are shown in Table 5.
표 5에서 보듯이, AST는 유황발효사료 급여군이 대조군에 비해 낮게 측정되었으나, ALT는 유황발효사료 급여군이 대조군에 비해 높게 측정되었다. AST와 ALT의 수치를 고려해 볼 때 유황발효사료는 간장 및 신장에 독성을 미치지 않는 것으로 판단된다. As shown in Table 5, AST was lower in the fermented milk feed group than in the control group, but ALT was higher in the fermented milk feed group than in the control group. Considering the values of AST and ALT, the sulfur fermented feed is considered to be toxic to liver and kidney.
총 콜레스테롤, 저밀도 콜레스테롤(LDL), 고밀도 콜레스테롤(HDL)은 대조군에 비해 유황발효사료 급여군에서 낮게 측정되었다.Total cholesterol, LDL cholesterol and HDL cholesterol were lower in the fermented milk fed group than in the control group.
2-5. 유황발효사료의 급여에 따른 육계 내 일반성분 분석2-5. Analysis of general components in broiler chicks fed with sulfur fermented feed
상기 2-1의 사양시험 종류 직후 각 처리군에서 평균 체중에 가까운 육계를 선발하여 탈모 처리 후 정강이 고기와 가슴고기의 무게를 1:1의 비율로 각각 적출하여 분쇄기로 분쇄한 것을 분석 시료로 하여 수분, 조단백질, 조지방 및 조회분, 콜레스테롤 함량을 농업기술실용화재단에 의뢰하여 분석하였다. 그 결과를 표 6에 나타내었다.Immediately after the type of the test described in 2-1 above, broiler chicks close to the average weight were selected from each treatment group, and the weight of the shank meat and chest meat after the unhairing treatment was individually extracted at a ratio of 1: 1 and pulverized with a grinder to obtain an analytical sample Moisture, crude protein, crude fat and crude ash, and cholesterol content were analyzed and commissioned by the Agricultural Technology Application Foundation. The results are shown in Table 6.
(Cholesterol, mg/100g)cholesterol
(Cholesterol, mg / 100g)
표 6에서 보듯이, 계육에서 단백질 및 지방함량은 대조군에 비해 유황발효사료 급여군에서 모두 낮게 나타났으며, 콜레스테롤 함량은 대조군과 유황발효사료 급여군 간에 유의차가 없었지만 유황발효사료 급여군이 대조군에 비해 낮게 나타났음을 알 수 있었다. As shown in Table 6, the protein and fat contents in chicken meat were lower in the fermented milk fed group than in the control group, and the cholesterol content was not significantly different between the control and the fermented milk fed group, Respectively.
2-6. 유황발효사료의 급여에 따른 육계의 지방산 조성2-6. Fatty acid composition of broiler chickens fed with sulfur fermented diets
상기 2-1의 사양시험 종류 직후 각 처리군에서 평균 체중에 가까운 육계를 선발하여 탈모 처리 후 정강이 고기와 가슴고기의 무게를 1:1의 비율로 각각 적출하여 분쇄기로 분쇄한 시료를 1g씩 취하여 순천대학교 동물자원과학과 육과학 실험실에서 gas chromatograph(HP 7890, Agilent Technologies, USA)를 이용하여 지방산 조성을 분석하였다. 그 결과를 표 7에 나타내었다.Immediately after the type of specimen test described in 2-1 above, broiler chicks close to the average body weight were selected from each treatment group, and the weight of shank meat and chest meat was extracted at a ratio of 1: 1 after unhairing treatment and 1 g of each sample was pulverized with a grinder Fatty acid composition was analyzed using a gas chromatograph (HP 7890, Agilent Technologies, USA) in the Department of Animal Resources Science, Sunchon National University. The results are shown in Table 7.
상기 표 7에서 보듯이, 계육에서 가장 많은 조성을 차지하는 지방산인 oleic acid는 대조군에서 43.50%, 유황발효사료 급여군에서 42.60%를 나타내었지만 처리구간 통계적인 유의차는 나타나지 않았다. As shown in Table 7, oleic acid, which is the highest fatty acid in the meat, showed 43.50% in the control group and 42.60% in the fermented milk feed group, but no statistically significant difference was found in the treatment period.
필수지방산에서 가장 많은 함량을 차지하는 linoleic acid는 대조군에서 14.18%, 유황발효사료 급여군에서 13.85%를 나타내었지만 처리구간 통계적인 유의차는 나타나지 않았다. Linoleic acid, which is the highest content of essential fatty acids, showed 14.18% in control group and 13.85% in sulfur fermented feed group, but there was no statistically significant difference between treatments.
불포화지방산인 Tetradecenenoic acid 및 Palmitoleic acid는 유황발효사료 급여군이 대조군에 비해 높은 함량을 나타내었다.Tetradecenenoic acid and palmitoleic acid, which are unsaturated fatty acids, showed higher contents in the fermented milk feed group than in the control group.
Claims (8)
a) adding sulfur, mica and selenium to the herbal extract, including water, mucilage, licorice and fennel, and mixing; b) low-temperature melting the mixture of step a) with stirring at a temperature of 38 to 45 DEG C for 45 to 60 days; c) mixing the melt of step b) with molasses, glucose, sodium and water and heating at a temperature of 90 to 110 ° C for 60 to 70 minutes; d) inoculating a heated fermentation broth of Lactobacillus casei and Acetobacter tropicalis into the heated product of step c), followed by fermentation to produce liquid fermented sulfur; And e) mixing the liquid fermentation sulfur of step d) and the by-products of cereals at a weight ratio of 1: 4 to 5, and then inoculating and fermenting the yeast culture broth to produce a fermented feed for livestock containing liquid fermented sulfur Way.
상기 a) 단계는 유황에 유황 100 중량부를 기준으로 운모와 셀레늄을 각각 45 내지 55 중량부를 물에 첨가하여 균질화한 다음, 유황 100 중량부를 기준으로 45 내지 55 중량부의 두충, 감초 및 회향을 포함하는 약재 추출물을 추가로 첨가하여 혼합하여 이루어지는 것을 특징으로 하는 액상발효유황이 함유된 가축용 발효사료의 제조방법.
The method according to claim 1,
Wherein the step a) comprises adding 45 to 55 parts by weight of mica and selenium to the sulfur to 100 parts by weight of sulfur on the basis of 100 parts by weight of sulfur, homogenizing the mixture and then adding 45 to 55 parts by weight of bivalvia, licorice and fennel, based on 100 parts by weight of sulfur, Wherein the liquid fermented sulfur-containing fermented feed is added to and mixed with a pharmacopoeial extract.
상기 a) 단계의 두충, 감초 및 회향을 포함하는 약재 추출물은 두충, 감초 및 회향을 추출용매 100 중량부에 대하여 각각 1 내지 3 중량부, 1 내지 3 중량부 및 0.5 내지 1.5 중량부로 혼합하여 추출한 다음, 추출액의 총 중량을 기준으로 추출액의 용매를 48 내지 52중량%로 농축하여 제조된 것을 특징으로 하는 액상발효유황이 함유된 가축용 발효사료의 제조방법.
The method according to claim 1,
The medicinal plant extracts containing bivalves, licorice and fennel in step a) are prepared by mixing 1 to 3 parts by weight, 1 to 3 parts by weight and 0.5 to 1.5 parts by weight of bivalves, licorice and fennel, respectively, with respect to 100 parts by weight of the extraction solvent Next, a method for producing a fermented feed for livestock containing liquid fermented sulfur, characterized in that it is prepared by concentrating the solvent of the extract liquid to 48 to 52% by weight based on the total weight of the extract liquid.
상기 c) 단계는 b) 단계의 용융물에 용융물 100 중량부를 기준으로 하여 당밀 90 내지 110 중량부, 포도당 40 내지 60 중량부, 나트륨 20 내지 30 중량부 및 물 4,000 내지 6,000 중량부를 혼합하여 이루어지는 것을 특징으로 하는 액상발효유황이 함유된 가축용 발효사료의 제조방법.
The method according to claim 1,
In step c), 90 to 110 parts by weight of molasses, 40 to 60 parts by weight of glucose, 20 to 30 parts by weight of sodium and 4,000 to 6,000 parts by weight of water are mixed with the melt of step b) based on 100 parts by weight of the melt Wherein the liquid fermentation sulfur is contained in the liquid fermented feed.
상기 d) 단계의 발효균주는 락토바실러스 카제이(Lactobacillus casei) 및 아세토박터 트로피칼리스(Acetobacter tropicalis)이 1:1의 중량비로 혼합된 혼합 균주인 것을 특징으로 하는 액상발효유황이 함유된 가축용 발효사료의 제조방법.
The method according to claim 1,
The fermenting feed for livestock according to claim 1, wherein the fermentation broth of step (d) is a mixture of Lactobacillus casei and Acetobacter tropicalis in a weight ratio of 1: 1. ≪ / RTI >
상기 d) 단계의 발효는 c) 단계의 가열물에 가열물과 동일한 중량의 발효균주를 접종한 후 28 내지 40℃의 온도에서 65 내지 80시간 동안 이루어지는 것을 특징으로 하는 액상발효유황이 함유된 가축용 발효사료의 제조방법.
The method according to claim 1,
Wherein the fermentation in step d) is carried out at a temperature of 28 to 40 DEG C for 65 to 80 hours after the fermentation strain of the same weight as that of the heated product is inoculated into the heated product of step c) A method for producing a fermented feed.
상기 e) 단계의 발효는 액상발효유황과 곡류부산물을 혼합한 혼합물에 혼합물 100 중량부에 대하여 0.05 내지 0.2 중량부의 효모 배양액을 접종한 후 28 내지 40℃의 온도에서 65 내지 80시간 동안 이루어지는 것을 특징으로 하는 액상발효유황이 함유된 가축용 발효사료의 제조방법.
The method according to claim 1,
The fermentation of step (e) is performed by inoculating 0.05 to 0.2 part by weight of yeast culture solution with respect to 100 parts by weight of the mixture of the mixture of liquid fermented sulfur and cereal by-products, at a temperature of 28 to 40 DEG C for 65 to 80 hours Wherein the liquid fermentation sulfur is contained in the liquid fermented feed.
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Cited By (7)
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CN108684936A (en) * | 2018-05-23 | 2018-10-23 | 宁夏绿健源生物科技有限公司 | A kind of glycyrrhiza extract fermented health-care feed and its production technology |
CN110235822A (en) * | 2019-07-10 | 2019-09-17 | 湖北省农业科学院农产品加工与核农技术研究所 | Living aquatic product Antistress agent and its production method and application method |
KR102027581B1 (en) * | 2018-05-02 | 2019-10-01 | 주식회사 평강비아이엠 | Method for producing a formulated feed |
KR20210087284A (en) * | 2020-01-02 | 2021-07-12 | 주식회사 정다운 | Manufacturing method of natural plant sulfur feed for raising sulfur duck |
KR20210144059A (en) * | 2020-05-21 | 2021-11-30 | 주식회사 파이브네이쳐스 | Method for manufacturing growth-enhancing supplement containing detoxified nano sulfur and method for breeding livestock using growth-enhancing supplement containing detoxified nano sulfur |
KR20220063345A (en) * | 2020-11-10 | 2022-05-17 | 박정옥 | Method of manufacturing feed additives mixed with complex microorganism |
KR102630273B1 (en) * | 2022-11-03 | 2024-01-29 | 권나영 | Organic red pepper cultivation method using herbal medicines |
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2015
- 2015-10-29 KR KR1020150151064A patent/KR101748368B1/en active IP Right Grant
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102027581B1 (en) * | 2018-05-02 | 2019-10-01 | 주식회사 평강비아이엠 | Method for producing a formulated feed |
CN108684936A (en) * | 2018-05-23 | 2018-10-23 | 宁夏绿健源生物科技有限公司 | A kind of glycyrrhiza extract fermented health-care feed and its production technology |
CN110235822A (en) * | 2019-07-10 | 2019-09-17 | 湖北省农业科学院农产品加工与核农技术研究所 | Living aquatic product Antistress agent and its production method and application method |
CN110235822B (en) * | 2019-07-10 | 2021-11-26 | 湖北省农业科学院农产品加工与核农技术研究所 | Anti-stress agent for living aquatic products, production method and use method thereof |
KR20210087284A (en) * | 2020-01-02 | 2021-07-12 | 주식회사 정다운 | Manufacturing method of natural plant sulfur feed for raising sulfur duck |
KR20210144059A (en) * | 2020-05-21 | 2021-11-30 | 주식회사 파이브네이쳐스 | Method for manufacturing growth-enhancing supplement containing detoxified nano sulfur and method for breeding livestock using growth-enhancing supplement containing detoxified nano sulfur |
KR20220063345A (en) * | 2020-11-10 | 2022-05-17 | 박정옥 | Method of manufacturing feed additives mixed with complex microorganism |
KR102630273B1 (en) * | 2022-11-03 | 2024-01-29 | 권나영 | Organic red pepper cultivation method using herbal medicines |
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