KR102457586B1 - Method of manufacturing feed additives mixed with complex microorganism - Google Patents

Method of manufacturing feed additives mixed with complex microorganism Download PDF

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KR102457586B1
KR102457586B1 KR1020200149068A KR20200149068A KR102457586B1 KR 102457586 B1 KR102457586 B1 KR 102457586B1 KR 1020200149068 A KR1020200149068 A KR 1020200149068A KR 20200149068 A KR20200149068 A KR 20200149068A KR 102457586 B1 KR102457586 B1 KR 102457586B1
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granules
feed additives
powder
complex microbial
compound feed
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박정옥
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K30/00Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/20Dehydration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/30Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
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Abstract

본 발명은, pH10 내지 pH12.5의 알칼리 이온수에 복합미생물 균주를 5:5의 비율로 배합하여 1차배합물을 형성하는 단계(S110)와, 1차배합물과 미강분말을 6:4의 비율로 배합하여 분말 고형제를 형성하는 단계(S120)와, 분말 고형제에 총 중량 대비 0.04 내지 0.06중량%의 MSM과 0.2 내지 0.25중량%의 곤충분말을 첨가하여 교반하고 숙성하여 2차배합물을 형성하는 단계(S130)와, 2차배합물을 일정 크기로 타정하여 과립제를 형성하는 단계(S140)와, 과립제를 열풍건조하여 수분을 제거하고, 일정 중량단위로 포장하는 단계(S150)를 포함하여, 복합미생물 군집으로 유익세균을 우점시켜 고밀도 비브리오균에 의한 질병을 예방하고 면역력을 증강하는 사료첨가제를 제공할 수 있는, 복합미생물 배합 사료첨가제 제조방법을 개시한다.The present invention comprises the steps of forming a primary mixture by mixing a complex microbial strain in alkaline ionized water of pH10 to pH12.5 in a ratio of 5:5 (S110), and mixing the primary mixture and rice bran powder in a ratio of 6:4 Forming a powder solid by mixing (S120), and adding 0.04 to 0.06% by weight of MSM and 0.2 to 0.25% by weight of insect powder based on the total weight to the solid powder, stirring and aging to form a secondary mixture Including the step (S130), the step of tableting the secondary mixture to a predetermined size to form granules (S140), hot air drying the granules to remove moisture, and packaging the granules in a predetermined weight unit (S150), including, Disclosed is a method for manufacturing a compound microbial compound feed additive, which can provide a feed additive that prevents diseases caused by high-density Vibrio bacteria by predominance of beneficial bacteria as a microbial community and enhances immunity.

Description

복합미생물 배합 사료첨가제 제조방법{METHOD OF MANUFACTURING FEED ADDITIVES MIXED WITH COMPLEX MICROORGANISM}Method of manufacturing compound microbial compound feed additive {METHOD OF MANUFACTURING FEED ADDITIVES MIXED WITH COMPLEX MICROORGANISM}

본 발명은 알칼리 이온수에 의해 초기 소독 기능을 제공하고, 자체 미생물로 박테리아 활성화를 저해하여 수질 오염을 예방하고 미생물의 번식을 강화할 수 있고, 복합미생물 균주에 의해 미생물을 제거할 수 있는, 복합미생물 배합 사료첨가제 제조방법에 관한 것이다.The present invention provides an initial disinfection function by alkaline ionized water, prevents water pollution by inhibiting bacterial activation with its own microorganisms, can enhance the reproduction of microorganisms, and can remove microorganisms by complex microbial strains, compound microorganisms It relates to a method for manufacturing a feed additive.

양어장 또는 양식장에서 사용하는 반죽사료는 사료찌꺼기로 인해 수질이 혼탁해지며, 수조 내에서 분해되어 양식어 성장에 해로운 암모니아성 분해물인 아질산으로 쉽게 변하여 생육환경이 나빠지는 현상이 발생되고 있다.Dough feed used in fish farms or farms has turbid water quality due to feed residues, and is easily converted into nitrite, an ammonia decomposition product that is harmful to the growth of aquacultured fish as it is decomposed in a water tank, thereby deteriorating the growth environment.

특히, 이와 같이 수질이 나빠지면, 어장의 유해한 균들이 많아져 어류 성장 저행와 병균 발생의 원인이 된다. In particular, when the quality of the water deteriorates as described above, harmful bacteria in the fishery increase, which causes slow growth of fish and the occurrence of germs.

예컨대, 양식장에서 치어를 넣기 전에 양식장 내부 건조를 통해 미생물과 잔존하는 유기체를 제거한 후 물을 채워 넣고 치어를 넣게 되는데, 양식장 내부 물과 토양에서 살아남는 유기체로 인해 세균성 박테리아가 생성이 되어 유해세균을 증가시키는 요인이 되고, 이후 어류의 아가미로 통해 세균성 침입으로 생존율을 낮춰 수확을 감소하게 되고, 배설물 또는 섭취가 안 된 사료의 침지로 인해 부패된 물과 사료와 세균성 박테리아까지 섞이어 수질이 악화되어 생육환경이 심각하게 나빠지게 된다.For example, before putting the fry in the farm, the microorganisms and remaining organisms are removed through drying inside the farm, and then water is filled and the fry are put. After that, the survival rate is lowered by bacterial invasion through the gills of the fish, reducing the harvest, and due to the immersion of excrement or uningested feed, the water quality deteriorates due to the mixing of spoiled water with feed and bacterial bacteria. The environment is seriously deteriorating.

이에, 양식장 사료에 있어, 경제성과, 충분한 영양공급원으로서의 기능과, 신선도와, 양식장 수질정화 기능과, 항병력을 향상시키면서, 사료제조와 저장에서 발생하는 면역기능에 관여하는 영양소의 손실을 최소화시키도록 제조되는 제품이 필요하다. Therefore, in the farm feed, economic performance, function as a sufficient nutrient supply source, freshness, farm water purification function, and anti-military power, while minimizing the loss of nutrients involved in the immune function occurring in feed production and storage You need a product to be manufactured.

한국 공개특허공보 제10-2014-0105941호 (어류 양식용 부상성(浮上性) 사료 및 그 제조 방법, 2014.09.03)Korean Patent Application Laid-Open No. 10-2014-0105941 (floating feed for fish farming and manufacturing method therefor, 2014.09.03)

본 발명의 사상이 이루고자 하는 기술적 과제는, 알칼리 이온수에 의해 초기 소독 기능을 제공하고, 자체 미생물로 박테리아 활성화를 저해하여 수질 오염을 예방하고 미생물의 번식을 강화할 수 있고, 복합미생물 균주에 의해 미생물을 제거할 수 있는, 복합미생물 배합 사료첨가제 제조방법을 제공하는 데 있다.The technical problem to be achieved by the spirit of the present invention is to provide an initial disinfection function by alkaline ionized water, to prevent water pollution by inhibiting bacterial activation with its own microorganisms, and to strengthen the reproduction of microorganisms, and to control microorganisms by complex microbial strains An object of the present invention is to provide a method for manufacturing a compound microbial compound feed additive that can be removed.

전술한 목적을 달성하고자, 본 발명은, pH10 내지 pH12.5의 알칼리 이온수에 복합미생물 균주를 5:5의 비율로 배합하여 1차배합물을 형성하는 단계; 1차배합물과 미강분말을 6:4의 비율로 배합하여 분말 고형제를 형성하는 단계; 분말 고형제에 총 중량 대비 0.04 내지 0.06중량%의 MSM과 0.2 내지 0.25중량%의 곤충분말을 첨가하여 교반하고 숙성하여 2차배합물을 형성하는 단계; 2차배합물을 일정 크기로 타정하여 과립제를 형성하는 단계; 및 과립제를 열풍건조하여 수분을 제거하고, 일정 중량단위로 포장하는 단계;를 포함하는, 면역력 증강과 질병예방을 위한 복합미생물 배합 사료첨가제 제조방법을 제공한다.In order to achieve the above object, the present invention comprises the steps of forming a primary mixture by mixing a complex microbial strain in alkaline ionized water of pH10 to pH12.5 in a ratio of 5:5; Forming a solid powder by mixing the primary mixture and rice bran powder in a ratio of 6:4; Forming a secondary mixture by adding 0.04 to 0.06% by weight of MSM and 0.2 to 0.25% by weight of insect powder to the solid powder, followed by stirring and aging; forming granules by tableting the secondary mixture to a predetermined size; and hot air drying the granules to remove moisture, and packaging the granules in a predetermined weight unit; provides a method for manufacturing a compound microbial compound feed additive for immunity enhancement and disease prevention, including a.

여기서, 복합미생물 균주는 바실러스 아밀로리쿼파시엔스 균주일 수 있다.Here, the complex microbial strain may be a Bacillus amyloliquefaciens strain.

또한, 곤충분말은 건조된 식용곤충을 분쇄하여 제공될 수 있다.In addition, the insect powder may be provided by pulverizing dried edible insects.

또한, 식용곤충은, 메뚜기, 누에번데기, 백강잠누에, 갈색거저리 유충, 쌍별 귀뚜라미, 흰점박이꽃무지 유충 또는 장수풍뎅이 유충 중 어느 하나 이상 선택될 수 있다.In addition, the edible insect may be selected from any one or more of grasshoppers, silkworm pupae, white silkworms, brown mealworm larvae, paired crickets, locust larvae, or long beetle larvae.

또한, 알칼리 이온수는 아미노산과 구연산을 사용하여 pH10 내지 pH12.5로 제조된 이온수일 수 있다.In addition, the alkaline ionized water may be ionized water prepared at a pH of 10 to 12.5 using amino acids and citric acid.

또한, 과립제를 용해시켜 액상으로 양식사료 첨가제로 사용하거나, 알칼리 이온수에 바실러스 서버틸러스균을 5:5로 배합하고 배양한 액상을 50 내지 100배로 희석하여 양식사료 첨가제로 사용할 수 있다.In addition, the granules can be dissolved and used as an aquaculture feed additive in a liquid form, or a liquid cultured by mixing Bacillus subertilus in alkaline ionized water at a ratio of 5:5 and diluting the cultured liquid by 50 to 100 times can be used as an aquaculture feed additive.

또한, 과립제는 친유성 코팅제로 피복되될 수 있다.In addition, the granules may be coated with a lipophilic coating.

본 발명에 의하면, 생육을 활성화하고, 자체 미생물로 박테리아 활성화를 저해하여 수질 오염을 예방하고 미생물의 번식을 강화할 수 있고, 침전시 배설물의 유해균을 분해하여 수질을 오염시키지 않도록 하고, 알칼리 이온수에 의해 초기 소독 기능을 제공하고, 중금속을 포함하지 않아 안정성이 확보되고, 복합미생물 균주에 의해 미생물을 제거하고, 유황의 독소 제거 기능 및 신진대사 활성화 기능을 상승시키는 효과를 제공한다.According to the present invention, it is possible to activate growth and inhibit bacterial activation with its own microorganisms to prevent water pollution and enhance the reproduction of microorganisms, to decompose harmful bacteria in excreta during sedimentation to prevent polluting the water, and by alkaline ionized water It provides an initial disinfection function, does not contain heavy metals, so stability is secured, removes microorganisms by complex microorganism strains, and provides the effect of increasing the toxin removal function and metabolic activation function of sulfur.

또한, 칼리 이온수에 바실러스 서버틸러스균을 배합하고 배양한 액상을 희석하여 양식사료 첨가제로 적용할 수 있는 효과가 있다.In addition, there is an effect that can be applied as an aquaculture feed additive by mixing the Bacillus subertilus bacteria in potassium ionized water and diluting the cultured liquid.

도 1은 본 발명의 실시예에 의한 복합미생물 배합 사료첨가제 제조방법의 순서도를 도시한 것이다.
도 2는 도 1의 복합미생물 배합 사료첨가제 제조방법에 의해 제조된 사료첨가제를 예시한 것이다.
도 3은 도 1의 복합미생물 배합 사료첨가제 제조방법에 의해 제조된 사료첨가제를 양식장에 적용한 예를 예시한 것이다.
도 4는 도 1의 복합미생물 배합 사료첨가제 제조방법에 의해 제조된 사료첨가제를 양계장에 적용한 예를 예시한 것이다.
1 shows a flowchart of a method for manufacturing a compound microbial compound feed additive according to an embodiment of the present invention.
Figure 2 illustrates the feed additive prepared by the method for manufacturing the compound microbial compound feed additive of Figure 1.
FIG. 3 illustrates an example of applying the feed additive prepared by the method for manufacturing the compound microbial compound feed additive of FIG. 1 to a farm.
FIG. 4 illustrates an example of applying the feed additive prepared by the method for manufacturing a compound microbial compound feed additive of FIG. 1 to a poultry farm.

이하, 첨부된 도면을 참조로 전술한 특징을 갖는 본 발명의 실시예를 더욱 상세히 설명하고자 한다.Hereinafter, embodiments of the present invention having the above-described characteristics with reference to the accompanying drawings will be described in more detail.

본 발명의 실시예에 의한 복합미생물 배합 사료첨가제 제조방법은, pH10 내지 pH12.5의 알칼리 이온수에 복합미생물 균주를 5:5의 비율로 배합하여 1차배합물을 형성하는 단계(S110)와, 1차배합물과 미강분말을 6:4의 비율로 배합하여 분말 고형제를 형성하는 단계(S120)와, 분말 고형제에 총 중량 대비 0.04 내지 0.06중량%의 MSM과 0.2 내지 0.25중량%의 곤충분말을 첨가하여 교반하고 숙성하여 2차배합물을 형성하는 단계(S130)와, 2차배합물을 일정 크기로 타정하여 과립제를 형성하는 단계(S140)와, 과립제를 열풍건조하여 수분을 제거하고, 일정 중량단위로 포장하는 단계(S150)를 포함하여, 복합미생물 군집으로 유익세균을 우점시켜 고밀도 비브리오균에 의한 질병을 예방하고 면역력을 증강하는 사료첨가제를 제공하는 것을 요지로 한다.The compound microbial compound feed additive manufacturing method according to an embodiment of the present invention comprises the steps of forming a primary mixture by mixing the complex microbial strain in alkaline ionized water of pH10 to pH12.5 in a ratio of 5:5 (S110), 1 A step of forming a powder solid by mixing the tea mixture and rice bran powder in a ratio of 6:4 (S120), and 0.04 to 0.06% by weight of MSM and 0.2 to 0.25% by weight of insect powder based on the total weight of the powdered solid. Adding, stirring, and aging to form a secondary mixture (S130), tableting the secondary mixture to a predetermined size to form granules (S140), drying the granules with hot air to remove moisture, and a predetermined weight unit It aims to provide a feed additive that prevents diseases caused by high-density Vibrio bacteria by predominance of beneficial bacteria with a complex microbial community, including the step of packaging (S150), and enhances immunity.

이하, 도 1 내지 도 4를 참조하여, 전술한 구성의 복합미생물 배합 사료첨가제 제조방법을 구체적으로 상술하면 다음과 같다.Hereinafter, with reference to FIGS. 1 to 4, the method for preparing the compound microbial compound feed additive having the above-described configuration will be described in detail as follows.

우선, 1차배합물 형성 단계(S110)에서는, pH10 내지 pH12.5의 알칼리 이온수에 복합미생물 균주를 5:5의 비율로 배합하여 1차배합물을 형성한다.First, in the primary mixture forming step (S110), the complex microbial strain is mixed in alkaline ionized water of pH10 to pH12.5 in a ratio of 5:5 to form a primary mixture.

여기서, 알칼리 이온수는 아미노산과 구연산을 사용하여 pH10 내지 pH12.5의 범위로 pH를 조절하여 제조된 이온수일 수 있고, 복합미생물 균주는 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens) 균주일 수 있다.Here, the alkaline ionized water may be ionized water prepared by adjusting the pH in the range of pH10 to pH12.5 using amino acids and citric acid, and the complex microorganism strain may be a Bacillus amyloliquefaciens strain.

한편, pH10 내지 pH12.5의 알칼리 이온수는 세척과 세정 특성을 갖으며, 내성력 및 소화력을 강화시키는 역할을 하는데, 예컨대 복합미생물 균주의 초기 소독을 수행하거나, 후속하여 배합되거나 첨가되는 조성물을 소독하는 기능을 할 수 있고, 복합미생물 균주, 특히 바실러스 아밀로리쿼파시엔스 균주는 토양과 물속에 유익미생물로 우점하는 우점종으로 작용하여 기생하는 유해균주를 분해하여 억제하고, 플랑크톤을 활성화하는데 기여한다.On the other hand, alkaline ionized water of pH10 to pH12.5 has washing and cleaning properties, and serves to strengthen tolerance and digestibility, for example, performing initial disinfection of complex microbial strains, or disinfecting compositions to be formulated or added subsequently. Complex microbial strains, especially Bacillus amyloliquefaciens strains, act as dominant species as beneficial microorganisms in soil and water to decompose and inhibit parasitic harmful strains, and contribute to activating plankton.

또한, 바실러스 아밀로리쿼파시엔스 균주는 양어장, 양식장 또는 양계장에서 발생하는 배설물 속에 유해균이 기생하지 않도록 하고, 수질 내의 박테리아를 제거하고, 오염물의 탁도를 낮춰 오염을 예방하고 소독하는 기능을 수행한다.In addition, the Bacillus amyloliquefaciens strain prevents harmful bacteria from parasitizing in excrement generated in fish farms, farms or poultry farms, removes bacteria in water quality, and reduces turbidity of contaminants to prevent contamination and disinfection.

이에, 바실러스 아밀로리쿼파시엔스 균주는 혐기성 및 호기성의 복합적인 균주로 발효를 증가시키며, 강력한 소독약이자 사료 본연의 역할인 영양제가 되어 번식을 활성화시키고, 외부의 유해균들을 우점하여 사료로서 적합하면서도 양어장 또는 양식장의 수질 정화 작용에 적합한다.Accordingly, the Bacillus amyloliquefaciens strain is a complex anaerobic and aerobic strain that increases fermentation, becomes a powerful disinfectant and a nutrient that is the original role of feed, activates reproduction, dominates external harmful bacteria, and is suitable as a feed while being suitable as a fish farm Alternatively, it is suitable for water purification in farms.

다음, 분말 고형제 형성 단계(S120)에서는, 앞서 제조된 1차배합물과 미강분말을 6:4의 비율로 배합하여 분말 고형제를 형성한다.Next, in the powder solid formulation step (S120), the previously prepared primary mixture and rice bran powder are mixed in a ratio of 6:4 to form a powder solid formulation.

미강은 쌀눈 또는 쌀겨로서, 비타민 B성분이 다량 함유되어 있으며, 가바, 식이섬유, 라이신, 리놀레산, 베타스테롤, 알파토코페롤, 옥타코사놀 등으로 구성되고, 성분으로는 탄수화물 21g, 단백질 6g, 지방 2.8g, 당류 10g, 나트륨 90mg가 함유되어 있고, 유황과의 상호작용을 통해 유황의 독소 제거 기능 및 신진대사 활성화 기능을 상승시키는 효과를 제공할 수 있다.Rice bran is rice bran or rice bran, and contains a large amount of vitamin B. It contains 10 g of sugar and 90 mg of sodium, and through interaction with sulfur, it can provide the effect of increasing the toxin removal function and metabolic activation function of sulfur.

다음, 2차배합물 형성 단계(S130)에서는, 앞서 제조된 분말 고형제에 총 중량 대비 0.04 내지 0.06중량%의 MSM(Methyl Sulfonyl Methane,식이유황)과 0.2 내지 0.25중량%의 곤충분말을 첨가하여 교반하고 숙성하여 2차배합물을 형성한다.Next, in the secondary mixture forming step (S130), 0.04 to 0.06% by weight of MSM (Methyl Sulfonyl Methane, dietary sulfur) and 0.2 to 0.25% by weight of insect powder based on the total weight of the previously prepared solid powder were added and stirred. and aged to form a secondary mixture.

MSM은 산성토양을 중화시키고 바닷물의 적조현상을 없애는 기능을 수행하는데, 유황과 산소는 생물의 호흡, 동화작용, 신진대사 등에 없어서는 안 될 가장 중요한 물질로서, 독소 제거 기능을 제거하고, 천연농약과 천연비료와 녹조적조예방치료제로 사용된다.MSM neutralizes acidic soil and eliminates red tide in seawater. Sulfur and oxygen are the most important substances essential for respiration, assimilation, and metabolism of living things. It is used as a natural fertilizer and as a preventive treatment for algae and algae.

즉, MSM은 각종 질병에 내성을 갖게 하고, 폐사하거나 사멸하지 않고 성체로 생육되도록 하고, 배설물에 의한 수질 오염을 정화시키는 소독 기능을 제공할 수 있다.That is, MSM can provide a disinfection function that makes it resistant to various diseases, allows it to grow into an adult without dying or dying, and purifies water pollution by excrement.

또한, 곤충분말은 주요 단백질 영양소 공급원으로서 건조된 식용곤충을 분쇄하여 제공되되, 식용곤충으로는 메뚜기, 누에번데기, 백강잠누에, 갈색거저리 유충, 쌍별 귀뚜라미, 흰점박이꽃무지 유충 또는 장수풍뎅이 유충 중 어느 하나 이상 선택될 수 있다.In addition, the insect powder is provided by pulverizing dried edible insects as a major protein nutrient source, and the edible insects include any of grasshoppers, silkworm pupa, white silkworm, brown mealworm larvae, paired crickets, white-spotted beetle larvae or long-lived beetle larvae. One or more may be selected.

특히, 식용 쌍별 귀뚜라미는 조단백질 62g, 조지방 15g, 식이섬유 9g, 회분 5g, 탄수화물 2g, 수분 7g으로 구성되어 있고, 단백질 함량이 62g, 지방함량이 15.5g중 불포화지방이 62%로 구성되어 있고, 칼륨(k)이 0.98mg/100mg, 인(p) 0.85mg/100mg, 필수 아미노산 283ppm이 함유되어 있어, 신진대사 및 생장에 유익한 영양소를 제공할 수 있다.In particular, edible pairwise crickets are composed of 62 g of crude protein, 15 g of crude fat, 9 g of dietary fiber, 5 g of ash, 2 g of carbohydrate, and 7 g of water. It contains 0.98mg/100mg of potassium (k), 0.85mg/100mg of phosphorus (p), and 283ppm of essential amino acids, which can provide beneficial nutrients for metabolism and growth.

다음, 과립제 형성 단계(S140)에서는, 앞서 제조된 2차배합물을 일정 크기와 일정 형상으로 타정하여 과립제를 형성한다.Next, in the granule forming step (S140), the previously prepared secondary mixture is tableted to a predetermined size and a predetermined shape to form granules.

예컨대, 도 2의 (a)에 예시된 바와 같이, 3mm 내지 4mm 길이의 고형 과립제 형태로 타정되거나, (b)에 예시된 바와 같이, 3mm 내지 4mm 직경의 환형 과립제 형태로 타정될 수 있다.For example, as illustrated in (a) of FIG. 2, it may be tableted in the form of solid granules having a length of 3 mm to 4 mm, or in the form of round granules having a diameter of 3 mm to 4 mm as illustrated in (b).

한편, 과립제의 타정시에 친유성 코팅제로 피복되도록 하여, 사료첨가제의 부력을 추가로 제공하여 일정 시간 부상하도록 하여 양어장 또는 양식장 바닥 한 곳에 쌓이지 않고 고르게 배식되도록 할 수도 있다.On the other hand, by coating the granules with a lipophilic coating agent during tableting, the buoyancy of the feed additive is additionally provided to float for a certain period of time so that the granules are evenly distributed without accumulating in one place on the bottom of the fish farm or farm.

예컨대, 친유성 코팅제는 파라핀 왁스, 유동 파라핀(liquid paraffin), 미정질 왁스(Microcrystalline wax), 페트로라텀(Petrolatum)과 같은 광물성 유지, 밀랍(Bees wax)이나 울 왁스(Wool wax)와 같은 동물성 유지, 카르나우바 왁스(Carnauba wax)나 칸델리라 왁스(Candelilla wax)와 같은 식물성 유지, 스테아린산(stearic acid)과 같은 포화 또는 불포화 지방산, 폴리에틸렌 왁스(polyethylene wax), 폴리프로필렌 왁스(polypropylene wax), 피셔-트롭슈 왁스(FT 왁스), 메타크릴산-메틸메타크릴레이트 공중합체(copolymer), 에틸아크릴레이트-메틸메타크릴레이트 공중합체, 부틸메타크릴레이트-(2-디메틸아미노에틸)메타크릴레이트-메틸메타크릴레이트 삼중합체(terpolymer), 에틸아크릴레이트-메틸메타크릴레이트-트리메틸아미노에틸 메타크릴레이트 클로라이드 삼중합체와 같은 합성 친유성 고분자, 및 이들의 조합으로 구성되는 군에서 하나 이상 선택될 수 있다.For example, the lipophilic coating agent is paraffin wax, liquid paraffin, microcrystalline wax, mineral oil such as Petrolatum, animal oil such as Bees wax or wool wax. , vegetable oils such as carnauba wax or candelilla wax, saturated or unsaturated fatty acids such as stearic acid, polyethylene wax, polypropylene wax, Fischer-Tropsch wax (FT wax), methacrylic acid-methyl methacrylate copolymer, ethyl acrylate-methyl methacrylate copolymer, butyl methacrylate-(2-dimethylaminoethyl) methacrylate - at least one selected from the group consisting of synthetic lipophilic polymers such as methyl methacrylate terpolymer, ethyl acrylate-methyl methacrylate-trimethylaminoethyl methacrylate chloride terpolymer, and combinations thereof have.

다음, 포장 단계(S150)에서는, 앞서 제조된 과립제를 열풍건조하여 수분을 제거하고, 일정 중량단위로 포장하여 상품으로 제품화한다.Next, in the packaging step (S150), the previously prepared granules are dried with hot air to remove moisture, and packaged in a predetermined weight unit to be commercialized as a product.

과립제의 포장지로는 직사광선을 차단하여 변질을 예방할 수 있도록 불투명 포장지가 이용될 수 있다.As a wrapping paper for granules, an opaque wrapping paper may be used to prevent deterioration by blocking direct sunlight.

한편, 앞서, 타정된 과립제 형태의 사료첨가제를 전제로 예시하였으나, 과립제를 용해시켜 액상으로 양식사료 첨가제로 사용하거나, 알칼리 이온수에 바실러스 서버틸러스균을 5:5로 배합하고 배양한 액상을 50 내지 100배로 희석하여 양식사료 첨가제로 사용할 수도 있다.On the other hand, previously, it was exemplified on the premise of a feed additive in the form of a tableted granule, but the granule was dissolved and used as an aquaculture feed additive in a liquid form, or Bacillus subertilis was mixed in alkaline ionized water at a ratio of 5:5 and cultured in a liquid form of 50 to It can also be diluted 100 times and used as an aquaculture feed additive.

도 3은 도 1의 복합미생물 배합 사료첨가제 제조방법에 의해 제조된 사료첨가제를 양식장에 적용한 예를 예시한 것이고, 도 4는 도 1의 복합미생물 배합 사료첨가제 제조방법에 의해 제조된 사료첨가제를 양계장에 적용한 예를 예시한 것이다.Figure 3 illustrates an example of applying the feed additive prepared by the method for manufacturing the compound microbial compound feed additive of Fig. 1 to the aquaculture farm, and Fig. 4 is a poultry farm using the feed additive prepared by the method for manufacturing the compound microbial compound feed additive of Fig. 1 An example applied to

이를 참조하여, 본 실시예에 의한 복합미생물 배합 사료첨가제 제조방법에 의해 제조된 복합미생물 배합 사료첨가제의 효과를 상술하면 다음과 같다.With reference to this, the effect of the compound microbial compound feed additive prepared by the method for manufacturing compound microbial compound feed additive according to this embodiment will be described in detail as follows.

도 3에서와 같이 새우양식장에 적용한 경우, 기존의 사료첨가제에 비해, 플랑크톤의 왕성한 활동과 번식으로 초록색을 띠고, 암모니아가 거의 검출되지 않아 비브리오균을 억제하여 생육환경을 개선하고 기존의 석회, 콜린, 비타민제 또는 미네랄을 사용하지 않고서도 생존율을 높여 사료비용을 절감할 수 있다.When applied to shrimp farms as shown in FIG. 3, compared to the existing feed additives, it has a green color due to the vigorous activity and reproduction of plankton, and almost no ammonia is detected, thereby improving the growth environment by suppressing Vibrio bacteria and improving the existing lime, choline , it is possible to reduce feed costs by increasing the survival rate without using vitamins or minerals.

도 4에서와 같이 양계장에서 알칼리 이온수에 바실러스 서버틸러스균을 5:5로 배합하고 배양한 액상을 50 내지 100배로 희석하여 양식사료 첨가제로 적용한 경우, 대조구와 비교하여 시험구의 산란계의 산란양과 산란율과 총중량이 높고, 파란율이 낮고, 계란의 크기가 일정하고, 양계의 건강상태가 양호하고 기생충이 없었다.As shown in FIG. 4, when Bacillus serertilus bacteria was mixed in alkaline ionized water at a ratio of 5:5 in a poultry farm and the cultured liquid was diluted 50 to 100 times and applied as an aquaculture feed additive. The total weight was high, the blue rate was low, the eggs were uniform in size, the poultry was in good health, and there were no parasites.

한편, 알칼리 이온수의 중금속 유해성검사 결과에 의하면, 알칼리 이온수는 Cu와 Zn과 Fe 이외의 섭취시 호르몬체계를 교란시키고 체내 축적되어 배출되지 않는 Cd, As, Hg, Pb, Cr, Cr6 + 또는 Ni의 중금속을 포함하지 않는 것으로 확인되었다.On the other hand, according to the heavy metal toxicity test results of alkaline ionized water, alkaline ionized water disturbs the hormonal system when ingested other than Cu, Zn, and Fe, and Cd, As, Hg, Pb, Cr, Cr 6 + or Ni It was confirmed that it does not contain heavy metals.

또한, 알칼리 이온수와 복합미생물 균주의 배합후 균주검사 결과에 의하면, 알칼리 이온수와 복합미생물 균주가 5:5의 배합비율로 형성된 1차배합물에서는, 세균수(박테리아) 밀도가 2.6*108 개/mL 내외이고, 진균수(곰팡이) 밀도가 7.5*104 개/mL 내외인 것이 바람직할 수 있고, 유해균인 대장균, 녹농균, 황색포도상구균 또는 바실루스세레우스가 분해되어 수질 내에 기생하지 않을 수 있다.In addition, according to the results of the strain test after mixing the alkaline ionized water and the complex microbial strain, the bacterial count (bacteria) density was 2.6*10 8 / mL, and it may be preferable that the number of fungi (mold) density is around 7.5*10 4 /mL, and harmful bacteria such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus or Bacillus cereus may not be parasitic due to decomposition.

따라서, 전술한 바와 같은 복합미생물 배합 사료첨가제 제조방법에 의해서, 생육을 활성화하고, 자체 미생물로 박테리아 활성화를 저해하여 수질 오염을 예방하고 미생물의 번식을 강화할 수 있고, 침전시 배설물의 유해균을 분해하여 수질을 오염시키지 않도록 하고, 알칼리 이온수에 의해 초기 소독 기능을 제공하고, 중금속을 포함하지 않아 안정성이 확보되고, 복합미생물 균주에 의해 미생물을 제거하고, 유황의 독소 제거 기능 및 신진대사 활성화 기능을 상승시키는 효과를 제공할 수 있고, 알칼리 이온수에 바실러스 서버틸러스균을 배합하고 배양한 액상을 희석하여 양식사료 첨가제로 적용할 수도 있다.Therefore, by the method for producing compound feed additive formulated with complex microorganisms as described above, it is possible to activate growth and inhibit bacterial activation with its own microorganisms to prevent water pollution and enhance the reproduction of microorganisms, and to decompose harmful bacteria in excrement during sedimentation. Avoid polluting water quality, provide initial disinfection function by alkaline ionized water, secure stability because it does not contain heavy metals, remove microorganisms by complex microbial strain, and increase sulfur toxin removal function and metabolism activation function It is possible to provide the effect of reducing the temperature, and it is also possible to apply as an additive to aquaculture feed by diluting the liquid cultured by mixing Bacillus subtilis in alkaline ionized water.

본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 가장 바람직한 일 실시예에 불과할 뿐이고, 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원 시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형예들이 있을 수 있음을 이해하여야 한다.The embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiment of the present invention, and do not represent all of the technical spirit of the present invention, so various equivalents that can replace them at the time of the present application It should be understood that there may be water and variations.

S110 : 1차배합물 형성 단계
S120 : 분말 고형제 형성 단계
S130 : 2차배합물 형성 단계
S140 : 과립제 형성 단계
S150 : 포장 단계
S110: first compound formation step
S120: powder solid formation step
S130: secondary compound formation step
S140: granule formation step
S150: Packing Step

Claims (7)

pH10 내지 pH12.5의 알칼리 이온수에 복합미생물 균주를 5:5의 비율로 배합하여 1차배합물을 형성하는 단계;
상기 1차배합물과 미강분말을 6:4의 비율로 배합하여 분말 고형제를 형성하는 단계;
상기 분말 고형제에 총 중량 대비 0.04 내지 0.06중량%의 MSM과 0.2 내지 0.25중량%의 곤충분말을 첨가하여 교반하고 숙성하여 2차배합물을 형성하는 단계;
상기 2차배합물을 일정 크기로 타정하여 과립제를 형성하는 단계; 및
상기 과립제를 열풍건조하여 수분을 제거하고, 일정 중량단위로 포장하는 단계;를 포함하는,
복합미생물 배합 사료첨가제 제조방법.
Forming a primary mixture by mixing the complex microbial strain in alkaline ionized water of pH10 to pH12.5 in a ratio of 5:5;
forming a solid powder by mixing the primary mixture and rice bran powder in a ratio of 6:4;
Forming a secondary mixture by adding 0.04 to 0.06% by weight of MSM and 0.2 to 0.25% by weight of insect powder to the solid powder, based on the total weight, followed by stirring and aging;
forming granules by tableting the secondary mixture to a predetermined size; and
Containing; removing moisture by drying the granules with hot air, and packaging the granules in a predetermined weight unit;
A method for manufacturing complex microbial compound feed additives.
제1항에 있어서,
상기 복합미생물 균주는 바실러스 아밀로리쿼파시엔스 균주인 것을 특징으로 하는,
복합미생물 배합 사료첨가제 제조방법.
According to claim 1,
The complex microorganism strain is characterized in that the Bacillus amyloliquefaciens strain,
A method for manufacturing complex microbial compound feed additives.
제1항에 있어서,
상기 곤충분말은 건조된 식용곤충을 분쇄하여 제공되는 것을 특징으로 하는,
복합미생물 배합 사료첨가제 제조방법.
According to claim 1,
The insect powder is characterized in that provided by pulverizing dried edible insects,
A method for manufacturing complex microbial compound feed additives.
제3항에 있어서,
상기 식용곤충은, 메뚜기, 누에번데기, 백강잠누에, 갈색거저리 유충, 쌍별 귀뚜라미, 흰점박이꽃무지 유충 또는 장수풍뎅이 유충 중 어느 하나 이상 선택되는 것을 특징으로 하는,
복합미생물 배합 사료첨가제 제조방법.
4. The method of claim 3,
The edible insects, characterized in that any one or more selected from grasshoppers, silkworm pupae, white silkworms, brown mealworm larvae, paired crickets, locust larvae, or long beetle larvae,
A method for manufacturing complex microbial compound feed additives.
제1항에 있어서,
상기 알칼리 이온수는 아미노산과 구연산을 사용하여 pH10 내지 pH12.5로 제조된 이온수인 것을 특징으로 하는,
복합미생물 배합 사료첨가제 제조방법.
According to claim 1,
The alkaline ionized water is characterized in that it is ionized water prepared at pH 10 to pH 12.5 using amino acids and citric acid,
A method for manufacturing complex microbial compound feed additives.
삭제delete 제1항에 있어서,
상기 과립제는 친유성 코팅제로 피복되는 것을 특징으로 하는,
복합미생물 배합 사료첨가제 제조방법.
According to claim 1,
The granules are characterized in that coated with a lipophilic coating,
A method for manufacturing complex microbial compound feed additives.
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