RU2708161C1 - Fodder complex biologically active additive for animals and birds - Google Patents

Abstract

FIELD: fodder production.

SUBSTANCE: invention relates to fodder production, in particular, to fodder complex biologically active additive for animals and birds. Additive contains powder of zeolite, a probiotic including biomass of lactic acid bacteria selected from Lactobacillus acidophilus, or Propionobacterium freundenreichii, or Bifidobacterium longum, or Bifidobacterium adolescentis, or Streptococcus termophilus with titer of not less than 10⁹ CFU/ml, fresh-water microalgae chlorella, feed glucose and cereal offal. All the initial components are taken at the specified ratio.

EFFECT: invention usage will allow to increase animal productivity and body immune status.

7 cl, 8 tbl, 9 ex

Description

The invention relates to the composition of feed additives for animals and birds and can be used in agriculture, biotechnology and veterinary medicine in the prevention of diseases and breeding of farm animals and birds.

Known feed additive "ZAMAKS-M" for animals, birds and fish, containing zeolite and elemental sulfur. Additionally, a sulfur-containing substance, for example methionine, is introduced into the feed additive. These components in the feed additive are contained in the following ratio of components, wt. %: zeolite 90-99.3; elemental sulfur 0.5-8; a sulfur-containing substance, for example, methionine 0.2-2. The proposed feed additive allows to increase the productivity of animals, birds and fish (RF patent No. 2115333, IPC A23K 1/175, 1998).

There is a method of obtaining a complex biologically active feed additive for sturgeon, which consists in the separate deep cultivation of strains of Cellulomonas uda ATCC 491, Bacillus subtilis VKPM B-8130 and Bacillus subtilis VKPM B-2984 on nutrient media of a given composition, the resulting cultures are mixed, carry out solid-phase fermentation in conditions of limited oxygen availability and dried to a moisture content of 8-10% (RF patent No. 2506810, IPC A23K 1/165, 02.20.2014).

It is known to use bacterial strains of Bacillus subtilis for the preparation of animal feed and feed additives (RF patents No. 2433738, IPC A23K 1/00, 11/20/2011; No. 2422506, IPC C12N 1/00, A23K 1/00, A23K 1/16, A23K 1 / 175, 06/27/2011).

Known strains of Bacillus subtilis with a high level of phytase production registered in DSMZ (Deutshe Sammlung von Mikroorganismen und Zellkulturen GmbH) under inventory numbers DSM 19466, 19467, 19489, on the basis of which a composition for feeding animals in combination with other additives is obtained (RF patent No. 2506307 , IPC C12N 1/20, A23K 1/16, 02/10/2014).

Known bacterial strain Bacillus subtilis IC-1435-1-1, providing restoration of microbiocenosis of the gastrointestinal tract of animals, having bactericidal, fungicidal and virucidal activity and deposited in the All-Russian Collection of Industrial Microorganisms FSUE GosNIIGenetika under registration number VKPM B-10641, on the basis of which receive VKPM B-10641 a preparation, in which a powdered sorbent from bran or zeolite is used as filler (RF patent No. 2482174, IPC C12N 1/20, А61К 35/74, 05/20/2013).

Known probiotic strain Bacillus subtilis 8130, on the basis of which a probiotic feed additive "Probiocel" was created, which affects the intestinal microflora, secretory and enzymatic activity of the gastrointestinal tract of animals and generally activates functional activity, improving metabolism, (RF patent No. 2346463 , IPC A23K 1/165, 02.20.2009).

A known probiotic preparation intended for the treatment and prevention of gastroenteritis of weaned pigs, based on the Bacillus subtilis strain TPI and the Bacillus lichemformis strain, is a mixture of culture fluids containing microbial synthesis products of these strains with a concentration of microbial cells of 5 bcm / cm ³ in a volume ratio of the indicated strains 1: 1, to which an equal mass mixture of bran and dicalcium phosphate was added (RF patents No. 2399662, IPC C12N 1/20, А23К 1/16, 09/20/2010; No. 2399661, IPC C12N 1/20 , A23K 1/16, 09/20/2010).

Known probiotic compositions for animals and birds, containing bacteria Rhuminococcus albus, Bacillus subtilis, taken in equal amounts, and a method for producing probiotic compositions (RF patents No. 2266747, IPC AK61/35/66, А23К 1/165, 12/27/2005; No. 2266126, IPC A61K 35/66, A23K 1/165, 12.20.2005).

A known probiotic additive containing the biomass of spore-forming bacteria Bacillus subtilis and a hydrophilic carrier of brand A and hydrophobic brand AM (RF patent No. 2252956, IPC C12N 1/20, A23K 1/165, A23K 35/66, 05.27.2005).

Known probiotic feed additive, including a mixture of bacterial biomass of strains of Bacillus subtilis VKPM 10172 and Bacillus licheniformis VKPM 10135 in equal proportions in spore form with a total amount of at least 2 × 10 ⁸ spores / g and a carbohydrate protector (RF patent No. 2437563, IPC A23K 1 / 00, 12/27/2011).

Known feed additive for broiler chickens containing a natural mineral - glauconite and made in the form of a dry powder, crushed to a particle size of not more than 0.1 mm, with the additional introduction of the biosporin probiotic (RF patent No. 2352135 C2, IPC A23K 1/00 , A23K 1/16, A23K 1/175, 04/20/2009).

Known organic-mineral feed additive containing filler and flint containing material, the additive of which contains diatomite from the Inza deposit of the Ulyanovsk region with a silica content of 81.30-85.60% or flask of the Dubinsky deposit of the Ulyanovsk region with a silica content of 84.10-89.62% having high porosity and hygroscopicity with the ability to accumulate physiologically active substances from the filler (RF patent No. 2199883, IPC A23K 1/16, A23K 1/175, 03/10/2003).

Known feed additive for cattle, including vegetable and grain components and a mineral additive in the form of blue clay, which is a natural sorbent and a cheap source of vital macro- and microelements and has high adsorption and ion exchange properties, (RF patent No. 2238659, IPC А23К 1 / 16, A23K 1/175, 10.27.2004).

Known feed additives for animals and birds, including natural zeolite (RF patents No. 2484640, IPC A23K 1/00, 06/20/2013; No. 2467591, IPC A23K 1/16, A23K 1/00, 11/27/2012; No. 2448472, IPC A23K 1/175, 04/27/2012; No. 2390254, IPC A23K 1/00, A23K 1/16, A23K 1/175, 05/27/2010; No. 2385623, IPC A23K 1/16, A23K 1/175, 04/10/2010; No. 2374896, IPC A23K 1/00, A23K 1/175, 12/10/2009; No. 2262863, IPC A23K 1/16, A23K 1/175, 10.27.2005).

Known feed additive for stimulating the growth of pigs containing filler and antibiotics, while it contains the adsorbent "Bazhedin" as a filler (RF patent No. 2386343, IPC A23K 1/00, A23K 1/17, A23K 1/175, 04/20/2010) .

Known medicinal feed additive from peat of the Bastyanovsky peat enterprise of the Sverdlovsk region with the addition of an alkaline component (RF patent No. 2475038, IPC A23K 1/00, 02/20/2013).

Known feed additive for pigs, which is a natural mineral of the Vodinsky deposit of the Krasnoyarsk district of the Samara region, containing 47.37% sulfur and 21.4% calcium (RF patent No. 2480025, IPC A23K 1/175, 04/27/2013).

Known feed additive for young cattle, including bentonite of the Donguz deposit, sunflower fuz and inorganic sodium selenite, and wheat bran as filler (RF patent No. 2497382, IPC A23K 1/16, A23K 1/175, 11/10/2013).

Known active coal feed additive to increase the productivity of laying hens, including a sorbent, which is used activated carbon obtained from softwood, having a particle size of 0.1 to 2 mm, in an amount of 400 g per 1 ton of feed (RF patent No. 2505069, IPC A23K 1/00, 01/27/2014).

Known probiotic feed additive used in the feed for farm animals and poultry, consisting of viable spores of spore-forming bacteria of the strain Bacillus subtilis and filler (RF patent No. 2458526, IPC A23K 1/16, C12N 1/20, 08/20/2012).

Known drug for the treatment and prevention of intestinal dysbacterial conditions in birds, containing inulin and fermentation of live microorganisms Lactobacillus acidophilus, Enterococcus faecium (RF patent No. 2410105 C1, IPC A61K 35/66, 01/27/2011).

A known probiotic feed additive containing microorganisms selected from the group consisting of Enterococcus faecium, DSM 16211, Lactobacillus reuteri, DSM 16350, and Lactobacillus salivarius ssp.salivarius, DSM 16351, Pediococcus acidilactici, DSM 162104, 28Berium No. 2372788, IPC A23K 1/00, A23K 1/18, 11/20/2009).

Known feed additive for young pigs containing a culture of Enterococcus faecium bacteria, plant materials and excipient (RF patent No. 2359465, IPC A23K 1/00, 06/27/2009). The feed additive contains a probiotic, which includes bacterial cultures of Bifidobacterium globosum, Bacillus subtilis, including Enterococcus faecium. Dry beet pulp was used as plant material, and zeolite as a filler.

Known feed additive with phytoprobiotic activity on a mineral basis, used in the composition of feed for farm animals and poultry and consisting of viable bacteria of the strain Enterococcus faecium, plant material and excipient (RF patent No. 2574689, IPC A23K 1/16, publ. 02/10/2016) . The filler used is diatomite in the form of burnt chips, as the viable bacteria of the Enterococcus faecium strain, the Enterococcus faecium 1-35 bacteria strain, deposited in the collection of the All-Russian State Research Institute for Control, Standardization and Certification of Veterinary Medicines (VGNKI) on March 20, 2008 under registration number Enterococcus faecium VGNKI 03/08/53 DEP-stored in the collection of microorganisms of "Biotroph" supported on diatomaceous earth in the form of burnt chips and having a titer of bacteria Enterococcus faecium least 1-35 June ¹⁰ KO / Mg. A mixture of essential oils of eucalyptus, thyme, garlic and lemon taken at a ratio of 1: 2: 1: 2, respectively, applied to diatomite in the form of burnt chips to obtain a dry concentrate of a mixture of essential oils of eucalyptus, thyme, garlic and lemon, is used as plant material in the form of a powder, while the feed additive has the following composition, g: dry concentrate of a mixture of essential oils of eucalyptus, thyme, garlic and lemon in the form of a powder of 50-150; bacteria deposited on diatomite in the form of burnt chips 100; diatomaceous earth in the form of burnt chips 850-750; the feed additive is added to the feed at a ratio of 0.001: 1. Viable bacteria Enterococcus faecium 1-35 contribute to the creation of normal microflora, increase digestibility and assimilation of nutrients in the diet.

The use of a mixture of essential oils of eucalyptus, thyme, garlic and lemon helps to suppress the development of pathogenic microflora in the gastrointestinal tract, preservation of antimicrobial properties and the manifestation of antioxidant properties.

The use of burnt crumb diatomite as a carrier when interacting with a mixture of viable bacteria Enterococcus faecium 1-35 and a dry concentrate of a mixture of essential oils of eucalyptus, thyme, garlic and lemon in the form of a powder normalizes digestion and improves health, improves the safety of young animals, and increase the productivity of farm animals and birds.

A known method of producing a biological product with probiotic properties for feeding farm animals and poultry, comprising a mixture of essential oils of garlic, eucalyptus, rosemary and thyme, and a bacterial strain Enterococcus faecium 1-35, and an excipient (RF patent No. 2458527, IPC A23K 1 / 165, 08.20.2012). Spore-forming bacteria of the genus Bacilus, whose common biological property is antagonistic activity against the opportunistic intestinal microflora of animals and the production of enzymes, improve feed absorption. The probiotic feed additive optimizes the microbial balance in the intestine due to the restoration of normal flora, and helps to increase the nonspecific resistance of the animal organism. The specified feed additive is made on the basis of the association of three types of spore-forming bacteria Bacillus subtilis VKPM B-2574, Bacillus licheniformis B-020, Bacillus cereus VKPM B-2492, filler and dispersant. Lactose, sucrose, skimmed milk powder are used as a filler, and Tween-80 is used as a dispersant. The use of a different composition in this analogue is not provided.

Known feed additive with probiotic activity on a mineral basis, used in the composition of feed for farm animals and poultry, consisting of viable spores of spore-forming bacteria of the strain Bacillus subtilis and filler. The strain of bacteria Bacillus subtilis 111, deposited in the All-Russian Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms named after G.K. Scriabin RAS (IBPM RAS) under registration number VKM B-2334 D, stored in the collection of microorganisms of BIOTROF LLC and consisting of viable spores of spore-forming bacteria Bacillus subtilis 111 with a titer of 2⋅10 ⁶ -6⋅10 ⁹ CFU / g, and in diatomite in the form of burnt chips with a mass ratio of viable spores of the spore-forming bacteria Bacillus subtilis 111 and diatomite in the form of burnt chips as 1:10, respectively, with the feed additive in the feed composition taken at a ratio of 0.001: 1 (RF patent No. 2569002 , IPC A23K 1/16, publ. 11/20/2015 g.).

Known granular feed, algolized using a product of processing a suspension of planktonic strains of chlorella, has biological activity and is a concentrate of biomass of chlorella cells with a cell density of 30 to 1000 million / ml. The product is contained in an amount of 2-3 wt. % at a cell density of chlorella from 50 to 500 million / ml. (RF patent No. 2538399, IPC A23K 1/00, publ. 01/10/2015).

Known feed additive from plant materials for animals, wt. %: beet pulp 15-20, sunflower husk 75-77.5, yeast autolysate 0.3-0.4 and chlorella suspension the rest up to 100 wt. % The microorganisms Trichoderma viride pc are added to the resulting mixture. F-98 in an amount of 1-2% by weight of the mixture and incubated for 6-8 days at a temperature of 26-30 ° C with periodic stirring, the resulting wet mixture is dried to a moisture content of 10-12% at a temperature of 40-50 ° C and ground (RF patent No. 2499410, IPC A23K 1/12, publ. November 27, 2013).

However, the above-mentioned feed additives containing chlorella have a long cooking cycle (6-8 days), and also have insufficient detoxifying and probiotic action due to the absence of components such as sorbents, for example zeolite and probiotic bacteria.

The closest analogue (prototype) is a biologically active feed additive for animals, birds and fish, containing zeolite as part of a standard diet, characterized in that it contains pre-made fractions of zeolite as zeolite: dust fraction with a particle size of 0.001-0.014 mm, dust fraction with a particle size of 0.014-0.3 mm, a granular fraction with a particle size of 0.3-1.0 mm in the following ratio, wt. %: dust fraction with a particle size of 0.001-0.014 - 1.0-85.0 mm; dust fraction with a particle size of 0.014-0.3 - 1.0-80.0 mm; granular fraction with a particle size of 0.3-1.0 mm (RF patent No. 2529706, IPC A23K 1/175, publ. 09/27/2014). As an alternative, the above biologically active feed additive may further comprise:

- elemental sulfur in an amount of 15-20 wt. %, which is introduced into fish food in the amount of 6 wt. %;

- or methionine in an amount of 3-5 wt. %, which is introduced into the feed for chickens in the amount of 6 wt. %;

- or vegetable raw materials selected from the group: wheat bran, seaweed, oats, chaga, wheat germ, dry grape extract, oatmeal, corn gluten in an amount of 1-5 wt. %, which is introduced into dry food for calves at a dose of 0.3-0.5 g / kg live weight;

- or a probiotic consisting of lactulose, in an amount of 3-5 wt. %, which is introduced into dry food for calves at a dose of 0.3-0.5 g / kg live weight.

The use of this invention will improve the efficiency of cleaning the body of toxic substances, by increasing the sorption properties of the feed additive, as well as increase the productivity of animals, birds and fish.

However, according to the above methodology for the use of a feed additive, the simultaneous combined use of such biologically active components as, for example, zeolite, bran, wheat algae and a probiotic is not provided for in this patent, which reduces the effectiveness and spectrum of action of this feed additive.

The technical result of the invention is to increase the efficiency and spectrum of the claimed feed additives by increasing the immune status of the body, achieved by the inclusion in the feed of the inventive feed complex additives, provided by increasing the productivity of poultry and other farm animals, reducing feed costs per 1 kg of live weight gain and increasing the economic effect in general due to the prevention of diseases, in particular mycotoxicosis.

The specified technical result is achieved by the fact that the feed complex dietary supplement for animals and birds, according to the invention, it contains a zeolite powder, a probiotic, including a biomass of lactic acid bacteria selected from Lactobacillus acidophilus, or Propionobacterium freundenreichii, or Bifidobacterium longum, or Bifidococobererium longum, or Bifidocobacterium bactrum termophilus with a titer of at least 10 ⁹ CFU / ml, freshwater microalgae chlorella, feed glucose and cereal bran, in the following quantitative ratio of the starting components, mass. %:

As a probiotic, the additive contains the biomass of the bacterial strain Lactobacillus acidophilus EP 317/402, or Propionobacterium freundenreichii shermanii Ac-103, or Bifidobacterium longum B-41, or Streptococcus termophilus TH-4 obtained on 1, 10, 20, 30 days of cultivation.

The supplement additionally contains a probiotic Biovestin. The biomass of the strain Lactobacillus acidophilus EP 317/402, or Propionobacterium freundenreichii shermanii Ac-103, or Bifidobacterium longum B-41, or

Streptococcus termophilus TH-4, obtained on the 1st, 10th, 20th, 30th day of cultivation and the probiotic Biovestin, were taken in a 1: 1 ratio. As a freshwater microalgae of chlorella, the additive contains the microalgae Chlorella vulgaris IGF C-111 or IBSS-19.

As a zeolite powder, the additive contains a modified zeolite obtained by heat treatment of a zeolite powder at a temperature of 450-650 ° C for 1.0-1.5 hours.

As bran cereal supplement contains wheat or a mixture of wheat and rye bran in a ratio of 3: 1.

As a zeolite powder, the additive contains a modified zeolite by heat treatment of the zeolite powder at a temperature of 450-650 ° C for 1.0-1.5 hours.

The principle of action of the declared biologically active feed additive affects the mechanisms of endogenous detoxification of xenobiotics, namely phase II, in which, as a result of a series of chemical reactions (methylation, trans sulfation), the metabolites are neutralized in the liver, forming less toxic and easily soluble conjugates, which are easily removed from the body. The presence of zeolite in the claimed additive helps to reduce the intensity of absorption of xenobiotics in the digestive tract and, together with the chlorella microalgae, can significantly reduce the toxigenic load on the body of birds and other animals during the forced feeding of poor-quality feed. Chlorella exometabolites, which are impregnated with a mixture of components of a complex feed additive, reduce its bacterial contamination and increase the shelf life of the product. In addition, the use of zeolite in the specified amount in the additive does not cause the effect of “unbalancing” feed, which is a specific concern when using only sorbents for this purpose.

As bran cereal supplement contains wheat or a mixture of wheat and rye bran in a ratio of at least 3: 1.

As a zeolite powder, the additive contains a modified zeolite by heat treatment of the zeolite powder at a temperature of 450-650 ° C for 1.0-1.5 hours.

The principle of action of the declared biologically active feed additive affects the mechanisms of endogenous detoxification of xenobiotics, namely phase II, in which, as a result of a series of chemical reactions (methylation, trans sulfation), the metabolites are neutralized in the liver, forming less toxic and easily soluble conjugates, which are easily removed from the body. The development of complex feed additives based on biologically active substances for the prevention of poultry poisoning with mycotoxins is physiologically justified, but it is even more pronounced when used together with sorbents. The presence of zeolite in the claimed additive helps to reduce the intensity of absorption of xenobiotics in the digestive tract and, together with the chlorella microalgae, can significantly reduce the toxigenic load on the body of birds and other animals during the forced feeding of poor-quality feed. Chlorella exometabolites, which are impregnated with a mixture of components of a complex feed additive, reduce its bacterial contamination and increase the shelf life of the product. Chlorella contains the acidic polysaccharide of chlorella chlon A, which has antitumor activity, stimulates the formation of interferon, and has a significant protective effect against influenza virus. In addition, the use of zeolite in the specified amount in the additive does not cause the effect of “unbalancing” feed, which is a specific concern when using only sorbents for this purpose.

For comprehensive protection of the body from mycotoxins, the use of the above components is enhanced by the additional inclusion of probiotic bacteria - donors of symbiotic microflora, since it is the primary barrier to the penetration of toxic substances into the body. Bacteria of the intestinal flora can not only prevent the absorption of toxic substances, but also participate in the processes of their microbiological destruction and inactivation.

The combined effect of all the components of the additive (chlorella, zeolite and symbiotic microflora) in these parameters allows you to effectively resist the negative effect of mycotoxins in the feed and prevent the development of acute and chronic forms of combined mycotoxicoses when feeding poor-quality compound feeds to the bird.

Probiotic bacteria are a functional food product containing, as a physiologically functional food ingredient, strains of living microorganisms that are beneficial for humans and animals (non-pathogenic and non-toxic) that beneficially affect the human or animal organism through normalization of the microflora of the digestive tract.

Probiotic bacteria help to increase digestive activity, normalize the metabolism of proteins, carbohydrates, fats due to more active formation and use of biologically active substances, enzymes, and neutralization of toxins.

The inclusion of a lactic acid feed additive in the inventive complex feed additive contributes (when feeding animals and poultry) to reduce intoxication with heavy metals and has a positive effect on the growth rate, the safety of birds and animals and the environmental safety of products.

As a result of the study of the enzymatic activity of MKD (Shvydkov A.N., Martyshenko A.E., Lantseva N.N., Chebakov V.P., Kobtseva L.A. RESEARCH OF ENZYMATIC PROPERTIES OF FODDER ADDITIVES // Successes in modern natural sciences. - 2014. - No. 11-2. - P. 49-53), it can be noted that all the studied MKD monocultures have a high degree of cellulose activity (CA) from 64.46 units / ml in MKD based on propionic acid bacteria and up to 72.4 units \ ml in MKD based on lactobacilli. All claimed MCDs contain an acidic protease in which protein hydrolysis is carried out at pH-5.5. The highest protease activity (PrA) was found in MCD based on propionic acid bacteria - 7.5 u / ml. The smallest PrA is contained in MKD based on lactobacilli 1.0 units / ml. Lipase activity (LA) was determined in MKD based on lactobacilli - 1.4 u / ml. Amylolytic activity (AA) was found in MCD based on propionic acid bacteria - 9.4 units / ml.

Thus, MKD based on propionic acid bacteria contains three groups of enzymes: amylolytic, proteolytic, cellulosalitic. Four groups of enzymes were found in MKD based on lactobacilli: proteolytic, cellulosalitic, lipolytic and amylolytic.

Propionibacterium freudenreichii - propionic acid bacteria, create an environment in which bifidobacteria grow, produce propionic and acetic acids, as well as substances that are active against enterobacteria, putrefactive bacteria, fungi, are involved in the fermentation of carbohydrates and vitamin formation, contribute to the absorption of vitamin B12.

Chlorella (Chlorella vulgaris) - a single-celled microscopic green algae, which contains a large amount of protein, a complete set of essential amino acids, carbohydrates, fats, vitamins and other biological substances. Chlorella is used as a protein source, has an anti-inflammatory, pronounced detoxifying effect against endogenous and exogenous toxins, stimulates the immune system, restores the function of the gastrointestinal tract in case of dysbiosis, and normalizes blood formation processes. Chlorella is recommended as a general strengthening agent, it prevents the development of neoplasms, strengthens the immune system, has antiseptic and regenerating properties, regulates cholesterol, as well as chlorella - the richest source of antioxidants.

The main active components of chlorella:

- Essential amino acids - are necessary for the formation of a protein that provides vital processes;

- vitamins (C, E, B1, B2, B3, B5, B6, folic acid, niacin, biotin, vitamin K) - stimulate the body's immune system, improve metabolism;

- micro and macro elements (calcium, zinc, copper, iron, magnesium) - polyunsaturated fatty acids - have anti-inflammatory and cardioprotective effects;

- fiber - has a detoxifying effect, improves intestinal motility;

- nucleic acids (RNA and DNA) - contribute to the renewal of cells of all organs and tissues of the body;

- enzymes - carry out and regulate biochemical processes in the body.

The main effect of chlorella:

- Immunomodulating - thanks to the chlorelan polysaccharide, which stimulates the production of interferon and increases the activity of macrophages, chlorella acts as an immunomodulator - strengthens the immune system.

- Detoxifying - due to the special structure of the cell wall, chlorella cleanses the body of endogenous and exogenous toxins (heavy metals - uranium, mercury, copper, lead; pesticides, insecticides, phenols): cellulose absorbs metabolic products, due to which chlorella neutralizes the effects of toxic substances and sporopollenin (a substance resistant to various enzymes) is a protective agent that acts against toxins.

- Antioxidant - thanks to a combination of nucleic acids, amino acids, peptides (glutathione) and vitamins, chlorella has a powerful antioxidant effect, prevents the aging process of body cells.

- Normalizing metabolism - chlorophyll and chlorella enzymes promote the absorption of vitamins, especially carotene, improve metabolism.

- Anti-inflammatory - polyunsaturated chlorella fatty acids help to cope with acute and chronic inflammatory processes of bacterial origin.

- Normalizing the intestinal microflora - one of the characteristics of chlorella is that in the process of its activity pathogenic bacteria die, and the growth of aerobic bacteria is stimulated. Chlorella restores the functions of the gastrointestinal tract with dysbiosis.

- Regulating the digestive tract - chlorophyll normalizes the secretion of digestive juices, relieves inflammation of the gastrointestinal tract, and increases the activity of its peristalsis. Chlorella cell wall fibers improve gastric function, eliminating indigestion.

- Improving the composition of the blood - chlorophyll, identical to the molecular structure of hemoglobin, a high content of vitamin B12, folic acid and iron improve blood formation processes, accelerate bone marrow regeneration. Chlorella is also the richest source of natural vitamin K.

The influence of natural zeolite as a component of the feed additive in animal husbandry and poultry farming (https://agroserver.ru/b/tseolit-dlya-zhivotnovodstva-558845.htm) are as follows:

1. Zeolite, acting as an adsorbent for metabolic products, mycotoxins, salts of heavy metals, radionuclides and other harmful substances, thereby contributing to the prevention of feed diseases.

2. Zeolite, participates as an ion exchanger in a number of biochemical transformations during digestion, including:

- in the transportation, activation and prolongation of the action of enzymes and hormones;

- in maintaining a favorable ionic balance for sodium, potassium and calcium;

- in stabilizing the acid-base balance in the digestive tract.

3. Zeolite as a catalyst promotes better assimilation by the body of macro- and micronutrients coming from food.

- Introduction to the diet of dry cows:

- animals have higher productivity after calving;

- Promotes healthy offspring.

Introduction to the diet of heifers and cows of the dairy herd:

- reduction in the number of abortions by 2-3%, reduction in culling due to infertility;

- reduction of calf mortality by 1.5 times;

- increase in calf yield by 6-8%;

- increase in milk yield per cow by 5-7%;

- increased fat content of milk 0.02-0.1%;

- cows spend less feed units per 1 kg of milk;

- in terms of the content of toxic elements, the milk obtained complies with biomedical requirements and sanitary standards for the quality of milk raw materials for the production of baby food. Introduction to the diet of pigs (sows, young pigs) contributes to:

- increase in multiplicity by 3-10%;

- increase in large-fruited by 4-12%;

- the appearance of farrowing in 96% of sows in the first day;

- increase in the growth rate of live weight of piglets-suckers by 3-7%;

- a decrease of 3-5 times the number of newborn piglets with gastroenteritis;

- reduction in times of death of born piglets;

- when feeding piglets with gastrointestinal disorders of an infectious etiology, it provides recovery for 3-5 days.

- Sheeps. Lamb lambs are prescribed from 5-7 days of age for free feeding according to the needs of the animal until the lambs graze in fresh grass;

The introduction to the diet of bright, fattening young animals, ewes contributes to:

- an increase in the shear of wool to 13% with an increase in its strength by 6-12%;

- increase in the multiplicity of ewes by 6-11%;

- increase in live weight of offspring by 3-14%.

Introduction to laying hens:

- increase in egg production by 2-8%;

- increase in the yield of egg mass per chicken by 3-7%;

- increase the strength of the shell.

The following are examples 1-7 of the compositions of the inventive feed complex biologically active additives.

Example 1. Feed complex biologically active additive (wt.%):

Zeolite powder 31,0 Glucose feed 3.45 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 13.8 Lactic acid supplement (MKD) based bacteria strain Lact. acidophilus EP 317/402 with a titer of at least 10 ⁹ CFU / ml obtained in different cultivation time in 1, 10, 20, 30 day 8.6 Probiotic Biovestin based strain Bifidobacterium adolescentis MC-42 bacteria with a titer of at least 10 ⁹ CFU / ml 8.6 Wheat bran the rest is up to 100%

Example 2. Feed complex biologically active additive (wt.%):

Heat treated zeolite powder 31,0 Glucose feed 3.45 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 13.8 The milk-sour additive (MKD) based on the strain Propionobacterium freundenreichii shermanii Ac-103 with a titer of at least 10 ⁹ CFU / ml obtained in different cultivation time in 1, 10, 20, 30 day 8.6 Probiotic Biovestin based strain Bifidobacterium adolescentis MC-42 bacteria with a titer of at least 10 ⁹ CFU / ml 8.6 Wheat bran the rest is up to 100%

Example 3. Feed complex biologically active additive (wt.%):

Zeolite powder 31,0 Glucose feed 3.45 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 13.8 Lactic acid supplement (MKD) based Bifidobacterium longum B-41 strain with a titer of at least 10 ⁹ CFU / ml obtained in different cultivation time in 1, 10, 20, 30 day 8.6 Probiotic Biovestin based strain Bifidobacterium adolescentis MC-42 bacteria with a titer of at least 10 ⁹ CFU / ml 8.6 a mixture of bran (3: 1) wheat and rye the rest is up to 100%

Example 4. Feed complex biologically active additive (wt.%):

Heat treated zeolite powder 31,0 Glucose feed 3.45 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 13.8 Lactic acid supplement (MKD) based strain Streptococcus termophilus TH-4 with a titer of at least 10 ⁹ CFU / ml obtained in different cultivation time in 1, 10, 20, 30 day 8.6 Probiotic Biovestin based strain Bifidobacterium adolescentis MC-42 bacteria with a titer of at least 10 ⁹ CFU / ml 8.6 a mixture of bran (4: 1) wheat and rye the rest is up to 100%

Example 5. Feed complex biologically active additive (wt.%):

Zeolite powder 30,0 Glucose feed 3.0 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 13.0 probiotic lactic acid biomass based on the bacterial strain Lact. acidophilus EP 317/402 with a titer of at least 10 ⁹ CFU / ml 18.0 Wheat bran the rest is up to 100%.

Example 6. Feed complex biologically active additive (wt.%):

Heat treated zeolite powder 32,0 Glucose feed 3,5 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 14.0

probiotic lactic acid biomass

based on the strain Propionobacterium freundenreichii shermanii Ac-103 with a titer of at least 10 ⁹ CFU / ml 14.0 Wheat bran the rest is up to 100%.

Example 7. Feed complex biologically active additive (wt.%):

Zeolite powder 31,0 Glucose feed 3.45 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 13.8

probiotic lactic acid biomass

based on a strain of Bifidobacterium adolescentis MC-42 with a titer of at least 10 ⁹ CFU / ml 10.0 Wheat bran the rest is up to 100%.

Example 8. Feed complex biologically active additive (wt.%):

Heat treated zeolite powder 31,0 Glucose feed 3.45 The microalga Chlorella vulgaris in the form of a suspension with a cell density of 100-1000 million / ml 13.8

probiotic lactic acid biomass

Based on Streptococcus termophilus TH-4 strain with a titer of at least 10 ⁹ CFU / ml 10.0 a mixture of bran (3: 1) wheat and rye the rest is up to 100%.

Example 9. Feed complex biologically active additive (wt.%):

Zeolite powder 31,0 Glucose feed 3.45 Chlorella vulgaris microalgae in suspension with a cell density of 100-1000 million / ml 13.8 The biomass of lactic acid probiotic bacteria based on a strain of Bifidobacterium adolescentis MC-42 with a titer of at least 10 ⁹ CFU / ml 18.0 Wheat bran the rest is up to 100%.

The technology of preparation of a complex feed biologically active additives (examples 1-9) and feed based on it. First, prepare or acquire the components of a complex feed additive (examples 1-9). Lactic acid feed additive (MCD) based on monocultures-MKD-B (Bifidobacter bifidum longum), MKD-S (Streptococcus termophilus), ICD-P (Propionobacterium acidipropionicum), MCD-L {Lactobacillus acidophilus) with a titer of not less than 10 ⁹ cfu / ml (TU 9224-001-0141853476-2008), obtained at different cultivation dates on the 1st, 10th, 20th, 30th day, is produced at Berdskaya Poultry Farm LLC (Novosibirsk Region), at which a site for the production of MKD was created (htttp: // Byrd-pf.rf / about.htm1).

A suspension or paste of the microalgae Chlorella vulgaris strain C-111 or IBSS-19 in the form of a suspension with a cell density of 100-1000 million / ml is produced in the digging of Microbiotechnologies LLC, Novosibirsk (https://mikrobiotehnologii.tiu.ru).

The probiotic Biovestin based on the bacterial strain Bifidobacterium adolescentis MC-42 with a titer of at least 10 ⁹ CFU / ml is produced by BioVesta LLC (http://www.biovesta.ru). The remaining probiotic bacteria (biomass of lactic acid bacteria Lactobacillus acidophilus, Propionobacterium freundenreichii, Bifidobacterium longum, Bifidobacterium adolescentis, Streptococcus termophilus with a titer of at least 10 ⁹ CFU / ml) are produced in JSC Vector-Biol, Novosibirsk .bialgam.ru).

Zeolite is made from high-quality natural zeolites of the Kholinsky deposit and is intended for use as a mineral, prophylactic additive in animal feed, birds and fish (http://www.zeolite.spb.ru/?yclid=1594853075747634996). The product is also used for the production of premixes and animal feed. Standard fractions: 0-3 mm, 1-3 mm, 3-5 mm (yield of the target fraction of at least 85%). Clinoptilolite content: not less than 45%. Zeolite powder can be modified by heat treatment at a temperature of 450-650 ° C for 1.0-1.5 hours, which improves its sorption ability by increasing the porosity of the material particles, which is achieved by more complete removal of absorption and crystallization moisture in the process heat treatment and increase the porosity of particles to 14.3-14.5% (instead of 3.1-3.5% of non-heat-treated material).

Feed glucose (dextrose, grape sugar) is obtained by hydrolysis from corn starch (GOST 975-88) increases the nutritional value of feed and biologically active additives, improves the functioning of the gastrointestinal tract of animals.

Wheat and rye bran are a by-product of flour milling, which is a hard shell of grain (GOST 13496.2-91).

To mix the components using a batch mixer VSPM-100. The mixer is turned on (the engine is started), bran is poured into the hopper, and then crushed zeolite powder is fed there, which ensures uniformity and uniformity of the mixture structure when adding liquid components. The mixture is stirred for 1.5-2 minutes until a homogeneous mass. While mixing the powder mixture in the mixer in a separate container, a suspension of microalgae is mixed with the biomass of lactic acid probiotic bacteria, followed by keeping the liquid mixture for 5 minutes.

Then, the rotor speed of the mixer is increased to such a value that the dry mixture forms a funnel with stirring. A mixture of a suspension of microalgae and probiotics is poured into the resulting funnel with a thin stream. The liquid stream must be thin, otherwise lumps of flour dust may form in the bran, which becomes very dense when dried. After the final introduction of the liquid components, feed glucose is poured into the mixer hopper evenly shaking it over the mixing mass. After the introduction of all components, the resulting mixture is additionally stirred for another 3-5 minutes. Next, the contents (wet mixture) with a thin layer (5-10 mm.) Spread on baking sheets and loaded into an aerodynamic air drying chamber. In an air drying chamber, the product is dried at a temperature of 38-42 ° C for 12 hours to a residual moisture content of not more than 3 wt. % and powder state of the product. The finished product is Packed in paper bags.

For the preparation of crushed feed with the addition of the inventive complex feed biologically active additives (examples 1-9) in an amount of 0.5-1.5 wt. % also use a batch mixer VSPM-100.

Example 10. The study of the effectiveness of the inventive feed complex dietary supplements in the technology of growing broiler chickens.

Methods of experimental work:

To conduct experimental work on the principle of analogues in live weight and age from broiler chickens (Ross-308) of 3 days old, 4 groups of 12 animals each were formed (Table 1). Individuals of all groups were grown in a cell battery, the conditions of detention (planting density, light and temperature conditions, air exchange) were the same for all groups and corresponded to zootechnical standards. Distribution of feed - manual, watering - automatic. The main diet for poultry of all groups was consistent with the recommendations of the poultry supplier. The main diet did not use enzymes, antibacterial substances, coccidiostatics, detoxicants, and animal feed and growth-promoting components. The diet of the experimental groups was compiled by multi-stage mixing of the inventive feed complex dietary supplement and the main diet. The duration of the experiment was 39 days, from 3 days of bird life to 42 days.

The safety of livestock for the period of the experiment. Over the period of the experiment, the safety of the livestock in all groups was maximum and amounted to 100%.

During the experiment, the following indicators were taken into account:

- livestock safety;

- increase in live weight during individual weighing at 3, 10, 20, 30 and 42 days of age;

- the amount of fed feed mixture and feed complex dietary supplement;

- slaughter yield of meat;

- results of a biochemical blood test;

- the state of microbiocenosis of the colon of the gastrointestinal tract at 42 days of age;

- the effect of feed complex dietary supplements on the antibiotic sensitivity of pathogenic microflora.

Weight gain during individual weighing.

The average live weight of broilers at 42 days of age was, in the control group, gr. No. 1 - 1993.92, in the experimental groups No. 2, No. 3, No. 4, respectively - 2127.33, 2076.83, 2043.25 g. Therefore, the increase in live weight in relation to the control group amounted to: No. 2 - 133.93 (+ 6.69%); No. 3 - 79.79 (+ 4.1%); and No. 4 - 47.52 (+ 2.4%), respectively, while during the experimental period the feed complex dietary supplement was fed: group No. 2 - 19.27 g., No. 3 - 38.58 g. and No. 4 - 57.58 g., For each head.

Considering the results of cultivation, it should be noted that the largest average daily gain in live weight was obtained in group No. 2 and amounted to 53.00 g, while in group No. 3 - 51.6 g. And group No. 4 - 50.8 g., But all experimental groups exceeded the control in this indicator, in which it was 49.6 g.

The amount of feed mixtures and feed complex dietary supplement.

Determining the effectiveness of the use of feed complex dietary supplements was calculated by the production of an increase in the live weight of broilers when fed 1 kg in the diet, and feed complex dietary supplements.

In the conditions of industrial production of poultry products, the influence of dietary components both on poultry productivity and feed conversion is important. Analysis of the results of the experiment showed that the highest feed costs for the production of 1 kg., Live weight gain were in the control group - 2.00 kg. The inclusion of a feed complex dietary supplement in the diet of broilers contributed to a decrease in feed consumption for increase in live weight. So, in group No. 2, this indicator amounted to 1.87 kg., Which is less than in the control by 6.5%, in group No. 3 - 1.92 kg. - 4.0% and group No. 4 - 1.94 kg. - 3%.

Thus, when feeding 1.0 kg. feed complex dietary supplement in group No. 2, at a dose of 0.5% by weight of feed, allows you to save 6.746 kg of feed (2.00 - 1.87: 0.01927). Increasing the dose of feed complex dietary supplements in the diet to 1.0% in group No. 3 allowed to reduce 2.074 kg. feed for each kilogram of feed complex nutritional supplements (2.00 - 1.92: 0.03858), up to 1.5% - saving was 1.042 kg. feed (2.00 - 1.94: 0.05758) (Table 5).

The slaughter yield of meat in groups corresponded to existing standards. There is also a tendency to decrease the slaughter yield of meat with an increase in the amount of feed complex dietary supplement in the diet. So, when 0.5% was included in the diet for broilers, this indicator was equal to 70.51%, and when 1.5% was included, it was 70.15% (Table 6).

One of the negative indicators in the cell cultivation of broilers is the presence of namin on the sternum. When examining the carcasses of the control group No. 1, the appearance of namines was revealed. In experimental groups No. 2, No. 3 and No. 4, the appearance of namines was not observed.

Biochemical blood test. A biochemical study of blood serum showed the effect of feed complex dietary supplements on the functional activity of the liver, which is in direct proportion to the entered percentage composition. In the control group of birds, the ACT level within the physiological norm was in 40% of the samples, with the introduction of 0.5% feed complex dietary supplements in 60.0%, and when feeding 1.0 and 1.5% in 100.0% of the studied samples A decrease in bilirubin was observed in the groups of use of the feed complex dietary supplement 0.5 - 1.5% to 7.48 - 7.97 μmol / L, while in the control group this indicator was at the level of 8.24 μmol / L. Strengthening the protein-synthetic function of the liver is confirmed by an increase in the level of serum albumin in the groups when feeding the feed complex dietary supplement in a concentration of 0.5-1.5%, where it was 36.2-37.0 g / l, while the indicator of the control group 33.82 g / l.

The phosphorus level in the control group is significantly higher than physiological norms (2.26 mmol / l), however, the use of feed additives in all concentrations reduced its level by 15.0-20.0%. The optimal value of calcium in 100.0% of the samples was noted when using 1.0% feed complex dietary supplement, which is 20.0% more than in the control group.

The state of microbiocenosis of the large section of the gastrointestinal tract at 42 days of age. A microbiological study of fecal samples of birds established the influence of the feed additive on the species composition of the intestinal microflora. It was found that the use of a feed complex dietary supplement in a concentration of 0.5-1.5% significantly reduces the content of clostridia in 40.0-60.0% and increases the presence of enterococci in 40.0-60% of samples of feces of birds (table. 7).

The most significant changes were revealed when 1.5% feed complex dietary supplement was introduced into the diet, where Klebsiella was observed in 80.0% of samples, which is 60.0% more than in other groups.

An analysis of the change in the antibiotic sensitivity of the intestinal microflora showed that the use of a feed complex dietary supplement in a concentration of 0.5 to 1.5% causes a decrease in the number of drugs to which microflora is sensitive, while the growth retardation diameter of microorganisms grows in groups 2 and 4, which contributes to the effectiveness of antibiotic therapy during veterinary measures (table. 8).

The results of the experiments showed that the use of the inventive feed complex dietary supplement has a positive effect on the viability and productivity of the bird. The use of a feed complex dietary supplement also positively affects the biochemical parameters of blood and the state of intestinal microbiocenosis.

The structure of the claimed feed complex dietary supplement is technological for use in an industrial poultry enterprise. Thus, the use in the diet of poultry feed complex dietary supplement allows you to:

- reduce the use of antibacterial agents (antibiotics);

- reduce the load on the body of the bird by reducing or eliminating in the diet of enzymes, animal feed, detoxifiers;

- improve feed conversion;

- optimize digestion;

- improve the quality of products and have a positive impact on the environment.

Thus, the claimed complex feed additive has, in comparison with the prototype, higher detoxification, antioxidant and sorbent properties, which is achieved due to the synergistic action of all its components (including zeolite, probiotics, chlorella microalgae, bran).

Claims (8)

1. A feed complex dietary supplement for animals and birds, characterized in that it contains a zeolite powder, a probiotic comprising a biomass of lactic acid bacteria selected from Lactobacillus acidophilus, or Propionobacterium freundenreichii, or Bifidobacterium termum adium termum adium troformium adium, not less than 10 ⁹ CFU / ml, freshwater microalgae chlorella, feed glucose and bran cereal, with the following quantitative ratio of the starting components, mass. %:

2. The additive according to claim 1, characterized in that as a probiotic it contains the biomass of the bacterial strain Lactobacillus acidophilus EP 317/402, or Propionobacterium freundenreichii shermanii Ac-103, or Bifidobacterium longum B-41, or Streptococcus termophilus TH-4, in 1, 10, 20, 30 day of cultivation. 3. The additive according to claim 1 or 2, characterized in that it additionally contains a probiotic Biovestin. 4. The additive according to claim 3, characterized in that the biomass of the strain Lactobacillus acidophilus EP 317/402, or Propionobacterium freundenreichii shermanii Ac-103, or Bifidobacterium longum B-41, or Streptococcus termophilus TH-4, obtained in 1, 10, 20 , 30 day of cultivation, and the probiotic Biovestin taken in a ratio of 1: 1. 5. The additive according to claim 1, characterized in that, as a freshwater microalga of chlorella, it contains the microalga Chlorella vulgaris IGF S-111 or IBSS-19. 6. The additive according to claim 1, characterized in that as the zeolite powder it contains a modified zeolite obtained by heat treatment of the zeolite powder at a temperature of 450-650 ° C for 1.0-1.5 hours. 7. The additive according to claim 1, characterized in that it contains wheat or a mixture of wheat and rye bran in a ratio of 3: 1 as cereal bran.