KR101904622B1 - The functionality feed additive and functionality feed manufacturing method - Google Patents

Abstract

The present invention relates to a composition for promoting the proliferation of microorganisms, which is separately cultivated, and a useful microbial fermentation microorganism is intensively cultivated by using a sugar nutrient culture liquid containing a sugar nutrient, The present invention relates to a method for producing a microorganism capable of promoting and activating proliferation and enhancing activity rate and survival rate and achieving a compound synergistic effect by binding of different microorganisms to one or more grains or grain byproducts containing rice bran, And fermented grain fermented by mixing with raw materials such as raw rice fermented by the nutrient and activated by the combined effect of beneficial microorganisms due to the effect of nutrient content is high digestion and absorption rate is significantly improved to improve the immune function of animals and fish, As the physiologically active substance contained in the circle The present invention relates to a method for producing a microorganism, which comprises culturing a complex microorganism as a feed additive for replacing antibiotics by cultivating microorganisms which can be widely used for aquatic animals such as cattle, pigs, chickens and aquatic animals such as fishes and abalones, Functional feed grain cereal fermentation additives were prepared,
A nutrient supplemented with a saccharide-containing microorganism including a beta-glucan component obtained from the microorganism or the grains for promoting immunological activity, and providing a functional feed capable of promoting growth by enhancing the immunity and simultaneously enhancing the nutrient source And a method for producing a functional feed using the same.

Description

TECHNICAL FIELD The present invention relates to a fermentation additive for cereal grains and a method for preparing the same,

The present invention relates to a functional feed grain fermentation additive comprising a mixed culture of a glycoform-containing microorganism and a method for producing a functional feed using the same,

More specifically, as a composition for promoting the proliferation of microorganisms cultured separately, useful microorganisms for sugar fermentation are concentrated in two stages by using a sugar nutrient culture solution containing sugar nutrients, and the usefulness and safety of the microorganisms are verified, The present invention relates to a method for producing a microorganism capable of promoting and activating proliferation and enhancing activity rate and survival rate and achieving a compound synergistic effect by binding of different microorganisms to one or more grains or grain byproducts containing rice bran, And fermented grain fermented by mixing with raw materials such as raw rice fermented by the nutrient and activated by the combined effect of beneficial microorganisms due to the effect of nutrient content is high digestion and absorption rate is significantly improved to improve the immune function of animals and fish, Physiologically active substance contained in the circle , Which is a feed additive for antibiotics substitution through microbial cultivation which can be widely used not only in animal husbandry but also in aquaculture such as cattle, pigs, chickens and aquatic animals such as fishes and abalones. , And fermented soybean meal

Functional feed grain cereal fermentation additive which can enhance the immunity and reinforce growth while supplementing deficient nutrient source of general feed added in feed including beta glucan component obtained from microorganism or cereal for improving immunity activity thus formed as active ingredient To a method for preparing a functional feed as a feed composition containing the same.

In the aquaculture culture, feed is the main factor that determines the success or failure of the aquaculture. Conventional feeds contain only the basic ingredients necessary for the growth of animals and fishes, and promote growth or contain special immunity enhancers It is not.

Feed additives that are added to the feed in trace amounts for nutritional or specific purposes are antibiotics, antibiotics, probiotics, trace minerals, and other additives that are added to the diet to prevent disease, supplement and grow, An active agent, an active agent, an organic acid agent, a flavoring agent, a sweetener, an antioxidant, various natural substances, and a functional substance may be classified as feed additives.

As a typical growth promoter among feed additives, antibiotics are effective in preventing diseases and improving productivity of livestock as well as lowering production costs. However, they are used in feeds such as livestock to reduce livestock immunity against diseases, And the drug remains in the livestock body, the use of antibiotic antibiotics added to the feed is limited due to the human health (safety) of the people who take it.

Therefore, various feed additives have been developed as alternatives to the use of antibiotics, which include enhancing the immunity of animals (β-glucan, chitosan, etc.), inhibiting the proliferation of harmful microorganisms by inducing healthy growth or environmental changes in the intestines , Oligosaccharides, probiotics, etc.). However, studies on efficacy and proper level of supplementation are still lacking. However, it is known that some herbal medicines and certain plant extracts have animal growth promotion, improvement of feed conversion ratio and anti-cancer effect These alternative substances are still known to be less effective than antibiotics in terms of effectiveness.

Recently, the safety problem of the use of growth promoters such as antibiotics has been attracting attention, and as a new method for replacing antibiotics, a study on a substitute substance stable to the resistance of microorganisms and a microbial additive have been most emphasized.

Particularly, as interest in well-being has increased recently, effective microorganisms are attracting attention in various fields such as fermentation of food, improvement of livestock feed, compost production, purification of animal wastes, improvement of soil quality, purification of wastewater.

Probiotics can be defined as living microbial additives that have beneficial effects on the host by feeding the livestock with beneficial bacteria in the intestinal tract and inhibiting the proliferation of harmful microorganisms and keeping the intestinal microflora in a balanced manner.

The use of natural materials using antimicrobial agents can be provided as a highly effective substitute for antibiotics. The addition of such microbial agents has not yet been found to cause any serious problems, and depending on the effect of addition, It is reported that the microorganisms are fixed and the digestibility is improved.

The microbial agents used for livestock are to keep the balance of intestinal microflora in the livestock, to increase the digestion and absorption rate of the feed, to prevent the disease and to enhance the productivity of livestock by strengthening the immune system. There is an action to activate.

However, as opposed to probiotics or antibiotics that have antibiotic substitution effects,

Studies on immunity enhancers have been carried out a lot, but only some probiotics are actually used in the field, but the use of immunopotentiators with excellent effects is still a problem.

Numerous microorganisms on the earth are subject to different culture conditions and characteristics. For example, microorganisms for purification treatment, microorganisms for deodorization, and microorganisms for manufacturing organic fertilizer are classified according to their characteristics and can not be arbitrarily applied to other fields Has become orthodoxy. That is, it is rather harmful to apply microorganisms to other fields other than the application fields of the microorganisms according to their characteristics.

Since the microorganism cultures are applied only to one field and can not be applied to other fields, the costs and the inconvenience of the microbes are increased when applied to various cases. And a variety of microorganism cultures are required. Therefore, a mixed probiotic agent that can expect various effects at the same time is being studied.

Probiotics are used to increase the productivity of industrial animals by replacing chemical antibiotics due to the effects of resistance to harmful infectious diseases, reduction of diarrhea, and excellence of immune activity, It has been used not only for industrial land animals such as pigs but also for growing fish. This expansion of the use of probiotics is due to the fact that antibiotics remain in the livestock products provided to humans, which may adversely affect the human body due to mutation of pathogens and the like, , And the substitution effect of antibiotics is recognized when these lactic acid bacteria are administered to animals and fish as probiotics instead of antibiotics.

However, this conventional probiotic technique is a mixture of a single microbial agent and several kinds of microbial agents, so that the efficacy effect is fragmentary or limited, and only a partial effect can be expected in various diseases of bowel disease

Diseases such as mad cow disease, which cause fatal diseases to humans, have been determined as a result of animal protein ingredients added to feeds, and the move to replace animal protein added to feeds worldwide with plant proteins is proceeding rapidly.

Among the vegetable protein raw materials that are being used as substitutes for animal proteins such as fish meal, meat meal or plasma in the feed market, feed grains with nutrient content, low cost, and edible availability are soybean meal, rice gruel, bran and corn have.

 As a low-cost, high-efficiency animal protein substitute protein source used as a vegetable protein, soybean meal (SBM), rice bran and wheat bran are the most economically obtainable vegetable protein sources. Unlike animal proteins, they are eco- Or protein in the feed, while proteins, fats, and carbohydrates in the feed usually form strong bonds with each other, resulting in a very low digestion and absorption rate, so that livestock or fish can absorb enough nutrients evenly I could not.

The GABA-rich rice bran is a byproduct of rice brown rice milling. It contains abundant amount of lipid, vitamin B group and high quality protein. It also contains abundant fiber and phosphorus. It has been reported that physiologically active substances having physiological activities such as diabetes and anticancer effects exist.

However, since the physiologically active substance of the rice ghoul exists in an insoluble form strongly bound to the plant cell wall, it is difficult to be used in living bodies. Therefore, it is necessary to convert the physiologically active substance into a water-soluble state by separating it from a plant cell wall so that it can be used in a living body.

GABA (γ-aminobytyric acid) is a kind of non-protein amino acid distributed in nature. It is a neurotransmitter that exists in the mammalian brain or spinal cord. It improves the blood flow of the brain, It is used as a therapeutic agent for the improvement of metabolism of the brain and the decrease of motivation. It promotes the brain cell metabolism function, and the nerve stabilization function, stress relieving, memory enhancement, blood pressure lowering effect, depression mitigation, It is also called brain food.

In addition, as a byproduct of milking from soybean, soybean meal containing soybean oil contains many anti-nutritional factors (ANF), which is a byproduct of plant high protein supply. When used as feed, digestibility is inhibited. In order to use as a feed, it is required to develop a new processing method capable of improving the quality of the soybean protein inexpensively, efficiently, and massively.

In particular, the feed industry is required to develop natural products with animal feed efficiency and growth promoting effects, and the food groups with immune enhancement and anticancer effects are constantly of interest. Recently, Beta Glucan has been attracting attention due to the tendency to prefer functional foods and good foods.

As well known, beta-glucan, which enhances the immune function of animals and humans, activates immune function of macrophages, natural killer cells, T-cells, etc. through nonspecific immune responses and inhibits the proliferation of cancer cells Beta-glucan derived from microorganisms isolated and produced from microorganisms such as mushrooms and yeast, and plant beta-glucan extracted and produced from grains of dietary fiber,

When beta-glucan is used in a health supplement or feed additive, it may exhibit an immunostimulating effect,

Although beta-glucan is abundant in barley, oats, mushrooms and rice bran, beta-glucan is a type of polysaccharide that is contained in the cell walls of yeast, mold, mushroom and cereals.

Therefore, there is an urgent need for the development of a feed additive having an improved feed efficiency due to the supply of a nutrient source and an excellent immunity, which is effective in preventing diseases and having an effect of reducing feed costs.

However, the conventional feed additives developed to improve the above problems have advantages and disadvantages for specific uses such as improvement of the breeding environment such as sterilization effect and growth promotion effect by strengthening of immunity, There is a problem in that a comprehensive effect due to multiple factors can not be realized.

Therefore, in the feed industry, much effort has been made to maximize the efficiency of the feed, and the development of various feed additives that can perform additional functions such as digestion and absorption enhancement have become important in the feed industry.

These prior arts are also described as follows. These prior arts also have problems in that they can not satisfy the comprehensive application requirement characteristics as a functional feed additive for realizing a comprehensive effect due to complex factors such as complicated disease causes .

Document 1: Korean Patent Laid-Open Publication No. 10-2012-0022291

(Livestock Feed Additive for Growth Containing Fermentation Products Using Biotite and Useful Microorganism, Published on March 12, 2012)

Document 2: Korean Unexamined Patent Publication No. 10-2003-0071236

(Compositional Amino Acid Composition for Growth Promotion and Animal and Fish Feeds Containing the same, Published on September 03, 2003)

Accordingly, the present inventors have made intensive efforts to solve the problems of the prior art, and as a result, the present inventors have made efforts to prepare functional grain cereal fermentation additives including a complex microbial culture as a feed additive for antibiotic substitution through microbial mixed culture,

The functional feed grains fermentation additive of the present invention, which is capable of enhancing immunity and enhancing growth by supplementing the deficient nutrient source of the general feed added to the feed including the beta-glucan component obtained from the microorganism for enhancing immunostimulatory activity and the grains, And a method for producing a functional feed as a feed composition containing the active ingredient has been completed.

As described above, the functional feed additives of the prior art have various problems,

 In the production of functional feed additives for feed addition, which can effectively be used for the prevention of disease and reduction of mortality, animal feed efficiency and weight gain of livestock animals and aquaculture,

A functional feed grain fermentation additive including a mixed microbial culture as a feed additive for replacing antibiotics through a mixed microorganism culture containing GABA-rich grains as a main fermentation source and a microorganism-containing microbial growth promoting nutrient,

Also, a functional feed grains fermentation additive capable of promoting growth by reinforcing the insufficient nutrient source of the general feed added to the feed including the beta-glucan component obtained from the microorganism or the graft for enhancing the immunological activity, The present invention has been made in view of the above problems, and it is an object of the present invention to solve the technical problem of developing a functional feed as a feed composition.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art,

As a composition for promoting the proliferation of different microorganisms, the useful microorganisms for sugar fermentation are concentrated in two stages by using a sugar nutrient culture solution containing a sugar nutrient, thereby proving its usefulness and safety. And one or more grains or grain byproducts containing the rice bran are mixed with the combined microbial activity culture which realizes the combined synergistic effect of activating and activating the various effects at the same time by increasing the activity rate and survival rate and by combining the different species with each other, By fermenting the fermentation source with the optimum composition of the culture medium and useful microorganism, the nutrient and the active microorganism of the grain fermenting raw material such as rice bran fermented, and the efficacy effect by the active microorganism, the nutrient content is high and the digestion and absorption rate is remarkably improved. Improve functionality The physiological activity is activated by the physiologically active substance contained in the fermentation source of water, so that it can be widely used for aquaculture such as cattle, pigs, chickens and other aquatic animals such as fishes and abalones. A functional feed grain fermentation additive comprising a complex microbial culture liquid is prepared as a feed additive,

Functional feed grain cereal fermentation additive which can enhance the immunity and reinforce growth while supplementing deficient nutrient source of general feed added in feed including beta glucan component obtained from microorganism or cereal for improving immunity activity thus formed as active ingredient It is an object of the present invention to provide a method for preparing a functional feed as a feed composition containing the same.

As described above, the functional feed grain fermentation additive including the mixed microbial culture with the sugar-added nutrient according to the present invention can be used as a feed additive in the feed including the beta-glucan component obtained from microorganisms or grains for promoting immunological activity. It is a functional feed grains fermentation additive which can reinforce insufficient nutrition source and simultaneously enhance the immunity to promote growth, thereby simultaneously acting as a mineral source and a probiotic agent.

When the livestock feed additives of the present invention are added to the feed to raise livestock or aquaculture fish, the growth and vitality of the livestock are enhanced and the immunity of the livestock is enhanced to increase the growth and vitality of the livestock, It can be effectively used to prevent diseases and reduce mortality of animals and fishes, to improve feed efficiency and weight.

In other words, the feed additive of the present invention improves the immune function of animal and human body, and contributes to skin health and intestinal health by improving the nutrient content and digestion and absorption rate by virtue of the nutritional property of the glycogen and the active beneficial microorganism. It helps to improve nutritional imbalance of livestock and health and promotes the physiological action such as immunity enhancement due to the physiologically active substance contained in the cereal fermenting material such as rice bran, and it can be widely used for the cultivation of land animals and fish animals .

Unlike the conventional microorganism preparation, the microorganism can be used as an activating nutrient in the cultivation of microorganisms, in which the grains and the functions of the microorganisms such as rice bran are activated as a fermenting substance so that the microorganisms can grow and proliferate explosively. And fermentation products produced by the coexistence of aerobic or anaerobic microorganisms and EM microorganisms by producing a multifunctional fermentation composition containing a complex microorganism culture having a variety of microorganisms having a strong variety of microorganisms.

The present invention can maintain the activity of microorganisms for a long period of time by coexisting aerobic microorganisms and anaerobic microorganisms as a combination of various kinds of lactic acid bacteria, bifidus bacteria and yeast bacteria, and is involved in various metabolic activities occurring in the body after ingesting food, Improvement of digestive power through secretion of digestive enzymes such as amylase, protease and lipase, improvement of diarrhea through inhibition of harmful bacteria, normalization of intestinal flora and inhibition of the formation of carcinogenic substances, inhibition of ammonia gas and hydrogen sulfide And a reduction in the amount of production, thereby achieving excellent bioavailability.

In addition, the present invention improves the immunity function of animals and humans by maintaining the conditions for producing beta-glucan by high-quality environmentally friendly culture. The feed composition of the present invention is characterized in that the content of beta-glucan is significantly increased Which is a high-quality vegetable protein source, has improved physiological activity compared to the physiological activity of the cereal fermenting material itself such as rice bran, thereby enabling the high-efficiency functional grain feed fermentation additive for immunological function improvement containing beta-glucan component , Good digestibility, strong anti-illness and high nutritional value.

Therefore, the feed additive of the present invention is a combination of the complementary synergistic effect of the mutually complementary function that simultaneously includes the beta-glucan derived from the microorganism, which is an immunologically active substance, and the plant beta-glucan extracted and produced from the dietary fiber of the grain, It is possible to effectively prevent and cure disease without antibiotics to be administered together to improve immunity in the case of feeding a general feed. As the protein content increases and low molecular weight increases digestion absorption rate and feed efficiency The weight of chicken, pig, fish and the like can be remarkably increased, and the productivity is greatly improved as compared with the prior art, so that it can be very usefully utilized in the feed industry.

As a result, the feed additive having an increased beta-glucan content of the present invention has no adverse effect according to the conventional chemical antibiotics and has a comparable effect on the growth rate (survival rate), average weight (growth rate), and feed conversion rate Therefore, it can be used as an antibiotic substitute feed additive. Especially, when an infectious disease is prevalent, it is useful for prevention or treatment and it can lower the mortality caused by the infection of the disease.

In order to achieve the above object, the present invention will be described in detail.

All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The present invention relates to a functional feed grain fermentation additive comprising a mixed culture of a glycoform-containing microorganism and a method for producing a functional feed using the same,

 A microbial cell growth promoting microbial nutrient which can be used as a carbon source necessary for microbial cell growth is prepared and then the useful microorganism for the fermentation of the saccharide is expanded and cultured in two stages by using the above- The nutrient broth was tested for its usefulness and safety, and it was found that the complex microbial activity was promoted and activated by increasing the activity rate and survival rate, Next, the complex microorganism culture solution is mixed with at least one grain or grain by-product fermenting agent selected from corn flour, wheat bran, and soybean meal containing rice bran enriched in GABA as a by-product of grain processing, Including a mixed culture As a feed additive for antibiotic substitution, a functional feed grain fermentation additive comprising a complex microorganism culture liquid was prepared,

Functional feed grain cereal fermentation additive which can enhance the immunity and reinforce growth while supplementing deficient nutrient source of general feed added in feed including beta glucan component obtained from microorganism or cereal for improving immunity activity thus formed as active ingredient To a method for preparing a functional feed as a feed composition containing the same.

In the present invention, the culture solution containing the glucose biomass for promoting microbial growth is prepared by mixing 30% by weight of sugar, 30% by weight of glucose, 20% by weight of potassium chloride, 5% by weight of sodium hydrogencarbonate with respect to 100% 5% by weight of acetyl salicylic acid, and 10% by weight of citric acid.

When the amount of the additive relative to the purified water is relatively small in the mixing ratio of the purified water containing the sugar nutrient of the present invention and the other additives, the efficiency of culturing the microorganisms is lowered.

When the sugar is less than 30% by weight based on 100% by weight of the purified water, the energy supply efficiency is lowered. If the sugar is excessive, the energy is wasted and the economical efficiency is lowered due to excessive sugar supply. If the amount of sodium hydrogencarbonate or acetyl salicylic acid is less than 5% by weight based on 100% by weight of purified water, the digestion and absorption rate will be lowered and a side effect will occur if it is contained in a large amount.

In the present invention, the culture solution containing the sugar nutrients for promoting microbial proliferation as described above is a culture solution containing, as an effective ingredient, an effective ingredient for increasing the efficiency of the microbial culture of the present invention, By maximizing the efficiency of microbial cultivation by synergistic effect, it is possible to improve the adaptability to the change of microbial culture environment, to grow healthy microbes healthy without infection of the disease throughout the culture period and to improve the growth rate, And thus can be used as an agent for promoting the proliferation and fermentation of microorganisms at low cost and quality.

In the present invention, the culture solution containing the glucose nutrients for promoting microbial proliferation is directly added to one or more sugar sources (cereal fermentation bodies) selected from rice bran, soybean meal, corn meal and wheat bran, which will be described later, In order to accelerate the growth of microorganisms, the microorganisms are injected into separate mixed microorganisms and expanded and proliferated. When the microorganisms are added and fermented to the fermented rice cereal grains, the adaptability and immunity of the microorganisms are increased Thereby enhancing the probability of survival of the microorganism.

The fermentation microorganism is used as a growth source for promoting the growth of microbial fermentation, which is necessary for the process of rapidly multiplying the culture liquid containing the above-mentioned saccharide nutrient. The fermenting microorganism is an anaerobic microorganism, aerobic microorganism, useful microorganism, (EM) complex culture to produce an activated complex microorganism culture medium,

The ratio of the weight ratio of each of the symbiotic microorganisms constituting the mixed microorganism culture solution to the mixed microorganism culture solution is composed of the same ratio,

A culture medium containing 2 to 3% of glucose-containing nutrients is added to the diluted sample of the complex microorganism at a ratio of 1: 50, and the mixture is first expanded for 30 to 50 hours at a temperature of 20 to 40 캜,

The culture medium containing 2 to 3% of glucose-containing nutrients is further added at a mixing ratio of 1: 50 by weight to the culture medium of the primary cultured microorganism as described above, and the microorganism is continuously grown for 30 to 50 hours at a temperature of 20 to 40 & The amount of the microorganism is increased so as to produce a complex microbial activity culture in which the microorganism is maximally proliferated as a high concentration of the complex strain culture medium for the optimal sugar fermentation.

Preferably, the microorganism is cultured at a temperature of 20 to 40 DEG C for 30 to 50 hours under the fermentation condition. When the fermentation is carried out at a temperature lower than 20 캜, there is a possibility that the germs may reproduce and the main germs may be difficult to grow during storage. When the germs are stirred at a temperature exceeding 40 캜, It may adversely affect the quality of the fermented product due to a somewhat higher water content.

The complex microorganism group used in the present invention includes various physiologically active substances such as photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, etc. directly or indirectly produced by low molecular polysaccharides and glycosides, peptides and bacteriocins. The useful microorganism is a complex organism, and the different microorganisms are combined and exert a powerful force.

As the culturing microorganisms of the present invention, all microorganisms such as anaerobes, aerobic bacteria, and useful microorganisms (EM) can be used together with all the flora and fauna living on the Korean Peninsula, A mixture of bacteria such as Bacillus subtilis, Bacillus thuringiensis, lactic acid bacteria, photosynthetic bacteria, yeast bacteria, gram-positive actinomycetes and fungi can be used. Photosynthetic bacteria include cyanobacteria, actinomycetes include Streptomyces, and molds include Aspergillus oryzae, Aspergilluspp., Penicillum, and the like. And more preferably Aspergillus oryzae or Lactobacillus acidophilus strain, which is an industrial filamentous fungus, which is a microorganism having a good sugar-fiber-decomposing ability and is known to have a high growth rate and an excellent carbohydrate-decomposing ability.

In particular, the strains used in the present invention can be used for the purpose of propagating and proliferating in the digestive tract contents of soil, livestock manure and livestock under different conditions such as composition, pH, temperature and moisture, It is very stable because it contains spore-forming ability by including a bacterium capable of propagating in the presence of vegetable fiber, and proliferates strongly in soil, livestock manure, and digestive tract.

A useful microorganism (EM) is a combination of several microorganisms beneficial to humans among many microorganisms present in nature. Yeast, lactic acid bacteria and photosynthetic bacteria constitute useful microorganisms, and the antioxidant ability of fermentation products produced by complex coexistence of these microorganisms is the effect of useful microorganisms.

Lactic acid bacteria that inhibit harmful bacteria will replace antibiotics used in diarrheal diseases, and aerobic microorganisms, which in themselves constitute the source of high protein and source of vitamin B group, Glucan (β-glucan) can be used as a dietary fiber for relieving constipation in the large intestine.

Bifidobacteria are antagonistic to the harmful bacteria present in the large intestine as well as lactic acid bacteria.

Bacillus subtilis produces decomposing enzymes that decompose carbohydrates, proteins, lipids, fibrin, and polyphosphate at high titer. Therefore, when mixed with feed of livestock, feeding increases digestion and absorption of feed, And reduces the stress caused by digestion, thereby improving the growth of livestock.

Each strain constituting the complex microorganism group of the present invention may use various microorganisms known to be beneficial as microorganisms for fermentation suitable for Korean characteristics, and the microorganisms may be obtained by using microorganisms existing in a natural state, using commercially available products It can also be used from a microorganism depository

As the microorganisms useful for the human body mutually bind to each other and expand into two stages, the activity rate and the survival rate are higher than when individual microorganisms are cultured independently.

In addition, one or more sugar sources (rice bran, corn meal, wheat bran, conglutin, etc.) including rice bran enriched in GABA as a by-product of grain processing were added to the culture medium containing the active microbial culture and the glucose- 3:10. The mixture was put into a container and stirred with a stirrer to give moisture to maintain the moisture content at 40%. The mixture was fermented at 20-40 ° C for 30-50 hours under the action of a fermenting microorganism. The functional grain cereal fermentation additive comprising the complex microorganism culture liquid as the feed additive for the antibiotic substitution of the present invention fermented with the sugar source as the complex microorganism culture solution using the glucose nutrients for promoting biological growth is produced.

As described above, cereal fermentation using a microorganism of a microorganism active culture medium as described above hydrolyzes nutrients such as starch to change it to sugar and utilizes the changed sugars as a nutrient source, thereby causing microbial growth to become active, The content of the substance and the organic acid can be further increased

In addition, the useful microorganisms in the human body are complementary to each other by mutually complementary binding, so that the fermentation period of the grain is effectively shortened by using the two-stage efficiently cultivated complex microbial activity culture and the mixing of anaerobic bacteria, aerobic bacteria, Aerobic bacteria are decreased by anaerobic bacteria and the anaerobic bacteria are increased by the culture medium, so that invasion or propagation of the germs causing the food spoilage is effectively suppressed, and various useful substances produced by various germs are contained in the final fermentation products.

When the moisture content is less than 40% in the fermentation condition, the growth of yeast and lactic acid bacteria is lowered and acid fermentation does not occur. When the moisture content is more than 40%, the decomposition rate of the organic material increases and anaerobic decomposition occurs. There is a problem that broken and effective fermentation by-products can not be obtained, and 40% of water is preferable in terms of function and preservability as a multi-function probiotics.

The fermented glycoprotein having a problem in the absorption rate of the vegetable high protein source or the physiologically active ingredient may be corn powder, soybean meal, wheat bran or the like in addition to the GABA-rich rice germ.

In the multistage fermentation process, the complex microorganisms are rapidly concentrated, and the carbohydrate contained in the nutrient-containing culture fluid is used as an energy source to proliferate the microorganisms. The enzyme protein is decomposed to digest and absorb the grains It is excreted in the degraded state, or the nutrients are synthesized and accumulated or secreted in the microorganism cells.

Among the complex microorganisms, Bacillus subtilis (Bacillus subtilis) is transformed into a protein that constitutes cells while actively growing using carbohydrates in the constituents of grain fermented products such as rice bran, corn, wheat bran, and soybean meal. The protein content is relatively increased during fermentation.

In addition, lactic acid bacterium produces a large amount of organic acid while growing vigorously with the raw material of grain, and not only shows the characteristic of decomposing the galacto-oligosaccharide contained in the grain, but also various polysaccharides can be hydrolyzed to prevent enteritis. The absorption rate of the component is remarkably improved.

As a result, the functional feed grain fermentation additive according to the present invention can effectively absorb the active ingredients of grain-like rice such as rice husk, which has low absorption rate due to shortage of intestinal microorganisms such as livestock, And increases the absorption of nutrients and functional components in the body.

In addition, the present invention provides a functional feed such as livestock or aquaculture by adding the above-described functional feed grain fermentation additive to commercially available general livestock or marine fish farming feed within the range of 5-15 wt% based on the total weight of the feed However,

Thus, the functional feed containing the beta-glucan component obtained from the microorganism for enhancing the immunoreactivity or the cereal grains is added to the functional feed grains fermentation additive which can reinforce the deficient nutrient source of the general feed added to the feed while enhancing the immunity, The present invention provides a method for producing a functional feed as a feed composition comprising as an active ingredient.

 When the feed additive of the present invention is added to the feed, if the feed additive is too small, the effect of addition is small and if the additive amount is too large, it becomes uneconomical and not preferable. Most preferably, the feed containing the functional feed additive of the present invention 10% by weight based on the total weight.

In addition to the above-mentioned functional food grain fermentation additive,

The extracts of fermented Hwangyu tree, which showed various physiological activities such as antioxidant, anti - cancer, antibacterial, anti - inflammatory, anti - diabetic and anti - atopy effects, By additionally and selectively adding to the feed in the range of 5 to 15 wt% relative to the total weight of the feed, by mixing the feed with each other so that the functional ingredients can be synergistically complemented without loss of the active ingredient The effect of increasing the β-glucan content by the effective cultivation of physiologically active substances and the stability of the feed additive with enhanced immunity promoting function were investigated. Antioxidant, anti-cancer, anti-bacterial, anti-inflammatory, anti-diabetic effect It is effective to prevent and treat diseases effectively without antibiotics because it can increase the self-immunity of livestock and aquaculture fish and improve feed efficiency. It also has the effect of increasing the protein content and absorbing digestion rate and feed efficiency by low molecular weight And can be implemented comprehensively.

The above-mentioned fermented extract of Hwigae-japonica can be obtained by crushing the washed Hwangchujanggwa (Leucaceae) leaf, the Hwigae-japonese branch and the germinated brown rice with a pin mill to a diameter of about 1 mm and then, by using a turbo mill, ) At a rotating speed of 6,750 rpm and air classification at an air classifying wheel speed of 7,500 rpm in an air classifier to determine the ultrafine powder type perilla leaf, 5% by weight of fermented microorganisms and wild type herbicides were mixed in a mixture of powdery raw materials at a ratio of 2: 1: 0.1 by weight, respectively, and fermented for 48 hours in an enzyme fermentation tank to enhance the pharmacological activity of It is preferable to prepare a fermented extract of Huangchu tree of the fermentation of pupae.

In the present invention, the fermented extract of Huangchu tree is fermented by a low-temperature extraction method which optimizes the active ingredients and fermented the leaves and branches of Huangchu tree to enhance the pharmacological activity of the Huangchu tree. The cell membrane is destroyed and the microorganisms are finely crushed and the active ingredient contained therein is dissolved in water and the dissolved fermentation product is formed so as to be easily absorbed in the body. Since the temperature is lower than that of the heat extraction method, the oil component is destroyed by heat It is possible to prevent deterioration.

In the present invention, the Dendropanax morbifera is a plant belonging to Araliaceae, and is a plant belonging to Araliaceae. It is a plant belonging to Araliaceae, and is a plant belonging to Araliaceae. It has diabetes, hypertension, blood circulation disorder, liver function, antioxidant, osteoporosis, periodontal disease, Anti-inflammatory and anti-cancer effects.

In addition, the extract of U. perchulatus of the present invention can be extracted using leaves, branches, fruits, roots, etc.

Since Huangchuang is a plant herbal medicine which has been proven to be safe for a long period of time, the Huanghui tree fermented product according to the present invention, which is made using the same as a raw material, has an advantage that a more effective pharmacological effect can be obtained without various side effects due to the use of a drug .

In the present invention, the above-mentioned conditions for producing the Fusarium oxysporum fermentation extract may be an optimization condition for fermentation. If these ratios are changed, the extraction efficiency of the Fusarium oxysporum effective ingredient through the fermentation extraction process may be lowered.

In addition, the functional feed additive of the present invention can be administered to animal or fish alone or in combination with other feed additives.

In the present invention, the animal in which the feed additive can be used is, for example, animal such as edible cow, cow, calf, pig, pig, sheep, goat, horse, rabbit, dog, cat, chick, , Poultry such as ducks, geese, turkeys, quails, small birds, and cultured fish such as flounder, duckweed, mullet, shrimp, and abalone.

The functional feed additives according to the present invention can also be used individually or in combination with conventionally known feed additives and can be used sequentially or simultaneously with conventional feed additives.

Example 1: Functional feed grains fermentation additive containing a mixed microbial fermentation solution containing glucose-containing nutrients and production of functional feeds using the same

1. Preparation of functional feed additives

1-1. Preparation of a culture containing glucose-containing nutrients for promoting microbial proliferation

30 wt% of sugar, 30 wt% of glucose, 20 wt% of potassium chloride, 5 wt% of sodium hydrogencarbonate, 5 wt% of acetyl salicylic acid and 10 wt% of citric acid were added to 100 wt% of purified water heated to 60 캜 And the mixture was stirred and heated to obtain a culture solution containing glucose-containing nutrients for promoting the proliferation of microorganisms.

1-2. Two-stage expansion culture of useful microorganisms for fermentation of sugarcane by using glucose nutrient culture

The composition ratio (weight ratio) of each of the anaerobic microorganism, the aerobic microorganism, and the beneficial microorganism (EM)

3% of the culture medium containing the glucose nutrients obtained in the above step 1 was added to the diluted sample of the complex microorganism at a ratio of 1: 50, and the mixture was first expanded for 30 hours at a temperature of 40 DEG C,

The culture medium containing 2 to 3% of glucose-containing nutrients was further added at a mixing ratio of 1: 50 by weight to the primary cultured microbial culture solution, and the culture was continuously expanded for 30 hours at a temperature of 40 DEG C , Which is a microorganism culture medium, was prepared.

1-3. Preparation of functional feed additives

As described above, in the culture medium containing the active microbial culture solution and the glucose-containing microorganism expanded and cultivated in two steps in the 1-1 and 1-2 steps, one or more glycosides including microcrystals containing GABA as a by- , Corn flour, conglutin, etc.) were mixed at a composition ratio of 300: 3: 10, and the mixture was placed in a storage container. While stirring with a stirrer, water was added to maintain the moisture content at 40% Temperature fermentation such that the microorganisms are rapidly proliferated as the amino acid or the organic substance is decomposed in the fermented body such as the yeast and the like to produce a functional feed additive fermented with the mixed microorganism culture solution using the biosynthesizing biosynthetic nutrient of the present invention.

2. Production of functional diets

The functional food additives prepared as described above were added to commercial commercial broiler chickens in an amount of 10 wt% based on the total weight of the feed to form a functional broiler chick.

Application Example: Functional feed grains fermentation additives including the fermentation extract of Huangchu tree and the mixed microbial culture of sugar-nutrient addition product and preparation of functional feeds using the same

Production of functional diets

1. Preparation of functional feed additives

A functional feed additive was prepared in the same manner as in Example 1 above.

2. Manufacture of Fermented Extract of Hwangchil

The washed and dried yellowtail leaf, the yellowing tree branch and the germinated brown rice were separately pulverized to a diameter of about 1 mm using a pin mill and then pulverized in a turbo mill at a beater rotational speed of 6,750 rpm Air classification at an air classifying wheel speed of 7,500 rpm in an air classifier to give a powder mixture of powdery materials such as ultrafine granular leaf, : 0.1% fermented extracts of fermented Phellodendron japonica with fermentation of 5% by weight of fermented microorganism and wild type herbaceous enzyme, respectively, and fermentation for 48 hours at low temperature in an enzyme fermentation tank. .

3. Production of a functional feed containing the combined culture of microorganisms and the fermented extract of Hwangyu tree

In the first step, the functional food additive composition and the fermented extract of Huangchu tree extracted in the second step were added to commercially available common broiler chicks based on the total weight of the feed in an amount of 5% by weight based on the total weight of the feed to form a broiler functional food.

Experimental example:

The broiler chickens aged 3 days after hatching were fed with the functional feeds of Examples 1 and 2 of the present invention and cultivated in poultry farms for 30 days. The results of the experiment are summarized in Table 1.

division Enforce Number of shipment Growth rate
(%) Shipping weight
(Kg) Average weight
(Kg) Feed quantity
(Kg) Feed rate Example 1 100 99 99 356 3.60 678 1.90 Example 2 100 100 100 362 3.62 669 1.85 Control
(No salary) 100 97 97 337 3.48 712 2.11

As can be seen in Table 1, the broilers fed the feeds containing the feed additives of Examples 1 and 2 of the present invention showed higher growth rate (survival rate), average weight gain, feed conversion ratio [ (Kg) / body weight gain (kg)] in the control group.

Therefore, when a functional feed additive of the present invention is used to produce a functional feed such as livestock or fish, the amount of feed such as livestock is improved and feed intake and feed conversion rate are improved as compared with conventional feed additives, I can confirm that it is improved.

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      As a result, the functional feed additive according to the present invention can be used as a feed additive that can replace antibiotics. As a nutrient source such as minerals and complex fungi, the digestion efficiency can be enhanced by acting as a probiotic agent capable of fixing in the digestive tract The antimicrobial and antioxidant functions of the fermented extract of Huangchul are complemented by the beta glucan component and the antibacterial antioxidant function of the extract. It has the function of activating the immune function, improving the disease resistance, and improving the intestinal motility, thereby promoting digestion and promoting growth.

Therefore, the present invention has no side effects due to chemical antibiotics and has an effect comparable to the growth rate (survival rate), the average weight (rate of growth), and the requirement rate of the feed as compared with the chemical antibiotic, It can be effectively used for fish disease prevention and reduction of mortality, feed efficiency and weight gain.

While the present invention has been described in connection with certain exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. .

Claims (5)

A method for producing a functional feed grain additive comprising a complex microbial culture,
30 parts by weight of sugar, 30 parts by weight of glucose, 20 parts by weight of potassium chloride, 5 parts by weight of sodium hydrogencarbonate, 5 parts by weight of acetyl salicylic acid, and 5 parts by weight of sodium chloride were added to 100 parts by weight of purified water heated to 50 to 60 DEG C so as to be used as a carbon source necessary for microbial cell growth. acid, 5 parts by weight of citric acid, and 10 parts by weight of citric acid to prepare a culture solution containing the saccharide-
The saccharide fermentation microorganism having a composition ratio of the weight ratio of the anaerobic microorganism consisting of lactic acid bacteria, yeast, Bacillus subtilis, and actinomycetes, the aerobic microorganism, and the beneficial microorganism (EM) Were grown in two steps to proliferate multiple complex microorganisms and to produce an activated microbial activity culture,
The mixed microorganism culture solution is mixed with one or more grains selected from the group consisting of corn flour, wheat bran, and soybean meal containing rice bran enriched in GABA as a byproduct of grain processing, A method for preparing a fermented functional grain grain fermentation additive comprising the steps of:
In the culture medium containing the active microbial culture and glycosaminoglycans as described above, cereal processing by-products were selected from wheat bran, soybean meal and corn meal
One or more fermenting yeast were mixed at a composition ratio of 300: 3: 10, put into a storage container, stirred with a stirrer and water was added to maintain the moisture content at 40%, and the fermentation microorganism activity at 20-40 ° C for 30-50 hours Which comprises fermenting a fermented cereal grains and a fermented microorganism active culture medium obtained by fermenting the fermented cereal grains at a low temperature, the fermentation method comprising the steps of: delete The method according to claim 1,
The complex microorganism active culture medium is prepared by adding a culture medium containing 2 to 3% of glucose-containing nutrients to a sample diluted 1: 50 with the complex microorganism at a temperature of 20 to 40 DEG C for 30 to 50 hours, Then,
The culture broth containing 2 to 3% of glucose-containing nutrients is further added at a ratio by weight of 1: 50 to the primary culture broth as described above, and the culture is continuously expanded for 30 to 50 hours at a temperature of 20 to 40 캜 Cultivating the microorganism as a culture medium for the optimum fermentation of sugarcane, and cultivating the microorganism active culture medium in which the microorganism is maximally grown, delete 4. The method according to any one of claims 1 to 3,
Wherein the functional food grain fermentation additive and the yellowtail tree fermentation extract are selectively contained in the range of 5 to 15 wt% based on the total feed weight,
The above-mentioned fermented extract of Hwigae-japonica can be obtained by crushing the washed Hwangchujanggwa (Leucaceae) leaf, the Hwigae-japonese branch and the germinated brown rice with a pin mill to a diameter of about 1 mm and then, by using a turbo mill, ) At a rotating speed of 6,750 rpm and air classification at an air classifying wheel speed of 7,500 rpm in an air classifier to determine the ultrafine powder-like Ulllabi leaf, Yellowish tree branches and germinated brown rice 5% by weight of fermented microorganisms and wild type herbicides were mixed in a mixture of powdery raw materials at a ratio of 2: 1: 0.1 by weight, respectively, and fermented for 48 hours in an enzyme fermentation tank to enhance the pharmacological activity of Wherein the fermented milk is a fermented extract of yellowtail tree of fermented pupae