KR101691364B1 - Bee subsidiary feeder composition comprising rice bran and manufacturing method thereof - Google Patents

Abstract

The present invention relates to an auxiliary feed composition for bees containing rice bran and a method for producing the same, comprising the steps of: (a) mixing rice bran flour and water at a weight ratio of 1: 0.05 to 0.3; (b) Bacillus bacteria in molasses (Bacillus sp . ), Yeast ( Saccharomyces sp . ), Lactic acid bacteria ( Lactobacillus sp . ) And actinomycetes ( Streptomyces sp . ); And a step of mixing at least one microorganism selected from the group consisting of microorganisms, (c) mixing molasses and water mixed with bacteria at a weight ratio of 1: 0.1 to 0.5; (d) adding the molasses mixture prepared in step (c) to the rice bran product prepared in step (a), stirring for 5 to 30 minutes in a rotary fermenter set at 35 to 38 ° C, and 30 to 6 hours Preparing a culture by conducting fermentation for 36 to 54 hours; And (e) drying the cultured product. Thus, 25 to 80 parts by weight of the composition is used for 100 parts by weight of the injection, thereby supplementing the nutritional components of the injection to increase the weight and the number of honey bees, .

Description

[0001] The present invention relates to an auxiliary feed composition for bees containing rice bran and a method for preparing the same,

The present invention relates to an auxiliary feed composition for a bee containing a rice gum, which can increase the weight and the number of bees as well as increase the production of honey, supplementing nutritional components of the injection, and a process for producing the same.

In general, the beekeeping industry utilizes the ecology and habits of bees to develop high-nutrient, high-quality beef by managing beekeeping such as specification management, breeding and production, distribution of products, remediation of diseases and pests, improvement of beekeeping apparatus, It is an industrial field that produces income by producing health food.

Honey is a monosaccharide mainly composed of glucose and sugar. It is directly absorbed into the barrier of the body and burned in the muscle cells of each part of the body. It provides not only the energy required for the activity of the living body, but also the glycogen State. It is a natural comprehensive food containing the necessary minerals in the body, which facilitates physiological action in the body and completes the development of bone tissue.

The beekeeping industry mentioned above is not only able to increase the income by a wide variety of products and rapid increase in demand, but also improves the yield by means of pollen of various crops, and is treated as a major agricultural policy in the world.

Beekeepers are looking for mushrooms scattered around the country in order to obtain high quality honey, and they are collecting honey. In order to obtain more honey, beekeepers who act as medium should be good and healthy bees.

In order to obtain such bees, it is necessary to supply nutritional feed as a cheap and suitable feed for the season, the rainy season,

In other words, when honey bees can not find their own food in the natural world, supply of artificial feed suitable for bees is desperate, but up until now, it has been necessary to supply edible sugar or a small amount of honey diluted, feeds using pollen, soybean meal, soybean protein, And the amount of supplementary feed supplied by the feeder.

When the above-mentioned edible sugar is fed, lack of vitamins, proteins, and minerals causes imbalance of nutrition, so honeybees become weaker and easily diseased, and activity is extremely lowered, so that a large amount of honey can not be obtained . In addition, there is a side effect that the weakness of the resistance must be used together with the therapeutic agent for the disease.

In addition, since it is impossible to obtain a cheap and high quality bee food to be fed to the honey bee, expensive honey bee feed is fed to the honey bee, thereby increasing the economic burden.

Therefore, there is a demand for a bee feed composition which is low in price, rich in nutrients, and capable of increasing the weight and the number of bees as well as increasing the production of honey.

Korean Patent No. 1319097 Korean Patent Publication No. 1995-0010768

It is an object of the present invention to provide an auxiliary feed composition for bees containing rice bran, which can increase the weight and the number of bees as well as increase the production of honey by replenishing the nutrients of the feed.

It is another object of the present invention to provide a method for preparing an auxiliary feed composition for bees.

In order to accomplish the above object, the present invention provides a method for preparing an auxiliary feed composition for bees, comprising the steps of: (a) mixing a rice bran and water at a weight ratio of 1: 0.05 to 0.3; (b) Molasses and Bacillus sp . ), Yeast ( Saccharomyces sp . ), Lactic acid bacteria ( Lactobacillus sp . ) And actinomycetes ( Streptomyces sp . (A) mixing at least one microorganism selected from the group consisting of (c) mixing molasses and water mixed with the bacteria at a weight ratio of 1: 0.1 to 0.5; (d) adding the molasses mixture prepared in step (c) to the rice gruel produced in step (a), stirring the mixture in a rotary fermenter set at 35 to 38 ° C for 5 to 30 minutes, ≪ / RTI > and fermenting for 36 to 54 hours to produce a culture; And (e) drying the culture.

In the step (a), the rice bran can be grown in a vapor of 100 to 150 ° C.

The squid cartilage powder may be added in the step (a), and the content of the squid cartilage powder may be 20 to 50 parts by weight based on 100 parts by weight of the rice husk.

In the step (b), molasses content may be 30 to 60 parts by weight based on 100 parts by weight of rice bran.

In the step (b), a waste bag may be added as a carbon source.

The clogged soil may be one wherein the clogged soil is suspended in water to have a concentration of 2 to 30%, and the mixture is stirred at 60 to 100 ° C for 30 minutes to 2 hours while adjusting the pH of the suspension to 6 to 9.

In step (d), the mixture of rice bran and molasses may be mixed in a weight ratio of 1: 0.02 to 0.1.

In step (d), the egg white peptide may be added during fermentation, and the content of the egg white peptide may be 5 to 20 parts by weight based on 100 parts by weight of the rice bran.

In step (e), the culture is inoculated with two strains of Bacillus subtilis (KCTC 3239) and Saccharomyces cerevisiae (KCTC 7915), followed by drying for 48 hours have.

The auxiliary feed composition for bees of the present invention to achieve another object above comprises 100 parts by weight of rice bran for the squid cartilage powder 20 to 50 parts by weight of egg white peptide of 5 to 20 parts by weight of Bacillus bacteria (Bacillus sp . ), Yeast ( Saccharomyces sp . ), Lactic acid bacteria ( Lactobacillus sp . ) And actinomycetes ( Streptomyces sp . ). ≪ / RTI >

The auxiliary feed composition for bees according to the present invention is used in an amount of 25 to 80 parts by weight based on 100 parts by weight of the injection, so that the nutrients of the injection can be supplemented to increase the weight and the number of bees and increase the production of honey.

In addition, since the auxiliary feed composition for bees of the present invention contains rice bran, squid cartilage powder, egg white peptide and the like, various natural ingredients are supplied to bees at the time of feeding, which is beneficial to the health of bees and is excellent in preference of bees.

In addition, since the auxiliary feed composition for bees of the present invention is a natural ingredient, resistance to chemicals is generated when a drug is used, problems such as excessive stress on bees due to the use of chemicals are not caused, Antibiotics can contribute to public health in general.

The present invention relates to an auxiliary feed composition for a bee containing a rice gum, which can increase the weight and the number of bees as well as increase the production of honey, supplementing nutritional components of the injection, and a process for producing the same.

Hereinafter, the present invention will be described in detail.

By the bees the secondary feed composition containing rice bran including rice bran of the invention is 100 parts by weight with respect to the squid cartilage powder 20 to 50 parts by weight of egg white peptide of 5 to 20 parts by weight of Bacillus bacteria (Bacillus sp . ), Yeast ( Saccharomyces sp . ), Lactic acid bacteria ( Lactobacillus sp . ) And actinomycetes ( Streptomyces sp . ). ≪ / RTI >

Since the rice bran contains various nutrients such as vitamin A, vitamin B1, vitamin B6, iron, and phosphorus mineral, it is possible to increase the weight and the number of bees and to increase the production amount of honey. Especially, It is suppressed and fresh flavor and taste are obtained, so that the preference of bees can be enhanced.

The rice bran powder may be rice bran oil prepared by heat-treated rice bran powder or carbon dioxide gas supercritical extraction method with an average particle diameter of 200 to 300 mesh, but it is preferably rice bran powder heat-treated with steam at 100 to 150 ° C.

Milling the rice bran powder by a roll mill, a pin mill or an air flow mill in a dry, wet or semi-wet manner so as to have an average particle diameter of 100 to 400 mesh; Heat-treating the pulverized corundum powder with steam at 100 to 150 ° C; And a step of selecting a powder having an average grain size of 200 to 300 mesh among the heat-treated coreless powder.

If the enzyme activity such as lipase is not completely inactivated, the rice bran powder may be reactivated due to reabsorption of moisture or the like to cause acidosis, so that the enzyme is inactivated through a heat treatment process to delay rancidity of the rice bran Not only does it soften rice bran, it will ferment well after fermentation, but also increase preference of bee.

The squid cartilage powder has an average particle size of 300 to 400 mesh, and produces chitin, chitosan, chitooligosaccharide and the like which are beneficial to bees during fermentation. Therefore, in case of using only rice bran, preference of honeybees is low. When squid cartilage powder and rice bran are used together, preference increases and honeybee population increases. On the other hand, when crab shells, shrimp shells and the like are used instead of squid cartilage powder, the number of bees does not increase and the yield of honey is rather reduced.

The content of squid cartilage powder is 20 to 50 parts by weight, preferably 20 to 30 parts by weight, based on 100 parts by weight of rice bran. When the content of squid cartilage powder is used within the above range, the preference degree and the number of bees can be increased.

The egg white peptide increases resistance to germs and lowers the mortality of bees. When used together with rice bran, the antimicrobial activity is higher and vitamin content of the supplementary feed composition is increased. The increase in vitamin content is due to the fermentation of rice bran and egg white peptides, resulting in an increase in the contents of vitamin A, vitamin B1 and vitamin B6 contained in rice bran.

The content of the egg white peptide is 5 to 20 parts by weight, preferably 5 to 10 parts by weight, based on 100 parts by weight of rice bran. When the content of the egg white peptide is less than the lower limit, the antibacterial activity may not be increased. If the content exceeds the upper limit, the honey bees' preference may be lowered and the honey production may be lowered.

As the above bacteria, Bacillus subtilis ) or Bacillus sp. sp . ); Saccharomyces ellipsoideus), to Saccharomyces Carlsbad Bergen sheath (Saccharomyces carlsbergensis), Hansen Lula cyano do (Hansenula anomaia), consisting of yeast M. Tess Ruud Cha (Saccharomycodes ludwigii) or Sheikh Candida (Candida sake) as Saccharomyces (Saccharomyces sp . ); Streptococcus lactis ) or Lactobacillus bulgaricus ( Lactobacillus < RTI ID = 0.0 > sp . ); And Streptomyces sp. , Which is Streptomyces griseus ; and at least one selected from the group consisting of Streptomyces sp .

The present invention also provides a method for producing an auxiliary feed composition for bees.

A method for preparing an auxiliary feed composition for bees according to the present invention comprises the steps of: (a) mixing a rice bran with water; (b) Bacillus bacteria in molasses (Bacillus sp . ), Yeast ( Saccharomyces sp . ), Lactic acid bacteria ( Lactobacillus sp . ) And Streptomyces sp .; And a step of mixing at least one microorganism selected from the group consisting of Streptomyces sp. (c) mixing molasses and water mixed with the bacteria; (d) adding a molasses mixture prepared in step (c) to the rice bran mixture prepared in step (a) to prepare a culture; And (e) drying the culture.

In step (a), the mixture of rice bran and water may be mixed at a weight ratio of 1: 0.05 to 0.3 to prepare a rice bran product, and a mixed rice powder mixed with the rice bran and squid rice bran powder may be used.

If the weight ratio of the rice bran and water is less than the lower limit, fermentation may not proceed, and if the weight ratio is above the upper limit, drying may be difficult.

On the other hand, in step (b), Bacillus sp. sp . ), Yeast ( Saccharomyces sp . ), Lactic acid bacteria ( Lactobacillus sp . ) And actinomycetes ( Streptomyces sp . ). ≪ / RTI >

The molasses is used as a carbon source at the time of culturing and is used in an amount of 30 to 60 parts by weight, preferably 40 to 50 parts by weight, based on 100 parts by weight of rice bran. If the content of molasses is less than the lower limit, the preference of bees may be lowered. If the content of molasses is higher than the upper limit, the amount of feed consumed by the bees may be decreased and the nutrition may be deficient.

In the step (b), the pulverized soil may be added as a carbon source at the time of culturing. The pulverized soil is suspended in water to a concentration of 2 to 30%, and the pH of the suspension is adjusted to 6 to 9, Lt; 0 > C for 30 minutes to 2 hours. The used backwash is used in an amount of 20 parts by weight or less, preferably 5 to 20 parts by weight, based on 100 parts by weight of molasses. When the content of pulverulent soil is added within the above range, the preference and the number of bees may increase as compared with the case of using molasses alone.

Next, in step (c), molasses and water mixed in the step (b) are mixed at a weight ratio of 1: 0.1 to 0.5, preferably 1: 0.2 to 0.4.

If the weight ratio of molasses and water mixed with the microorganism is less than the lower limit, fermentation may not proceed, and if the molar ratio exceeds the upper limit, drying may be difficult.

Next, in step (d), the molasses mixture prepared in step (c) is added to the rice gruel produced in step (a), and the mixture is stirred for 5 to 30 minutes in a rotary fermenter set at 35 to 38 ° C, The culture is prepared by repeating fermentation for 36 to 54 hours by repeating the fermentation for 30 minutes to 6 hours. In step (d), egg white peptides may be added during fermentation.

The rice bran product and the molasses mixture are mixed at a weight ratio of 1: 0.02 to 0.1. If the rice bran product is less than the lower limit value, the fermentation may not be performed. If the rice bran product is higher than the upper limit value, the bee population may not increase.

The culture cultured with the rice bran increased the vitamin content compared to the rice bran before culturing and produced chitin, chitosan, and chitooligosaccharide, thereby increasing the weight and the number of honey bees and increasing the production amount of honey have.

Next, the culture in step (d) is dried in step (e).

The culture may be dried immediately with a drier, but the culture may be supplemented with Bacillus subtilis My process by Sergio subtilis (KCTC 3239)) and non-saccharide Jia (Saccharomyces cerevisiae (KCTC 7915)), and drying for 48 hours is preferable for increasing the weight and population of bees.

When boiled soybeans, soybean protein or soybeans instead of fermented rice according to the present invention, it does not help increase the number of seaweeds when fermented without fermentation, and soybean meal is extremely poor in palatability. , There is a risk that the life of the bar is shortened.

The auxiliary feed composition for bees produced according to the present invention is used in an amount of 25 to 80 parts by weight based on 100 parts by weight of the injection preparation, and the content thereof varies according to the season. The injection is not particularly limited as long as it is a material used for honey bee feed. Examples of the injection material include a flower pot, a flower pot substitute, and an energizing agent.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Production Example 1. Preparation of rice bran powder

The rice bran was crushed by roll milling to have an average particle size of 100 to 400 mesh, and then treated with steam at 120 ° C for 15 minutes. Then, rice bran having a diameter of 200 to 300 mesh was selected to obtain rice bran flour.

Example 1.

100 parts by weight of the rice bran powder prepared in Preparation Example 1 and water were mixed at a weight ratio of 1: 0.2 to prepare rice bran products. On the other hand, molasses (40 parts by weight relative to 100 parts by weight of rice bran) and Bacillus subtilis ), and the mixture was mixed with water at a weight ratio of 1: 0.25 to prepare a molasses mixture.

Then, the mixture of rice bran and molasses was mixed, and 10 minutes of agitation and 3 hours of agitation were repeated in a rotary fermenter set at 35 to 38 ° C to conduct aerobic and anaerobic fermentation for 48 hours, followed by drying, Lt; / RTI >

At this time, the mixture of rice bran and molasses was mixed at a weight ratio of 1: 0.04.

Example 2.

The procedure of Example 1 was followed except that 10 parts by weight of pulverized wheat was added to 100 parts by weight of molasses in the preparation of the molasses mixture to prepare an auxiliary feed composition for bees.

In order to equalize the particles of the scavenging soil, the scavenging soil was suspended in water so as to have a concentration of 25%, and the suspension was stirred at 80 ° C for 1 hour while adjusting the pH of the suspension to 6 to 9 It is.

Example 3.

An auxiliary feed composition for bees was prepared using the mixed powder including the rice bran powder and 20 parts by weight of squid cartilage powder having an average particle size of 300 mesh.

Example 4.

An egg feed protein composition was prepared in the same manner as in Example 1 except that the egg white protein was added in an amount of 10 parts by weight based on 100 parts by weight of rice bran.

Comparative Example 1

The procedure of Example 1 was followed except that soybean meal was used instead of rice gruel to prepare an auxiliary feed composition for bees.

Comparative Example 2

The procedure of Example 1 was followed except that beef yeast was used instead of Bacillus subtilis to prepare an auxiliary feed composition for bees.

<Test Example>

In the following Test Example, 50 parts by weight of the supplementary feed prepared in Examples and Comparative Examples were mixed and tested with 100 parts by weight of a syrup containing pollen and an active agent.

Test Example  1. Evaluation of palatability

After feeding the feed containing the auxiliary feed for bees prepared in Examples and Comparative Examples, the palatability of bees was evaluated. 5 kg of feed containing 1 kg of diets containing supplementary feeds of Examples and Comparative Examples were packed into 5 bean sausages [Gangwon-do, Yeongwol-gun, Yeongwol-gun, Yeoncheon-myeon, Yeongcheon-gun, Gyeonggi-do, The palatability test was carried out over a period of 2 weeks for a total of 300 bottles, each of 10 bottles, and the results are shown in Table 1 below.

division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Excellent 27 30 33 buckets 26 buckets Seven 11 Excellent 18 containers 17 11 18 containers 19 buckets 10 Normal Five Three Six Six 20 buckets 23 buckets Poor 0 0 0 0 Four Five Very bad 0 0 0 0 0 One

As shown in Table 1 above, the feed compositions containing the supplementary feed composition prepared according to Examples 1 to 4 of the present invention were superior to Comparative Examples 1 and 2, and thus showed excellent palatability.

On the other hand, Comparative Examples 1 and 2 were found to be generally excellent and few to many.

Test Example 2. Weight gain experiment of bees

The bees were weighed two weeks after feeding the feed containing the supplementary feed for bees prepared in Examples and Comparative Examples, and the results are shown in Table 2 below.

division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Feeding total body weight (mg / grain) 43 43 42 43 42 43 Weight after feeding (mg / grain) 49 51 56 54 38 40

As shown in Table 2, the weights of bees fed the feed containing the supplementary feed composition prepared according to Examples 1 to 4 of the present invention were higher than those of the bees fed the compositions of Comparative Examples 1 and 2 Respectively.

On the other hand, it was confirmed that the weights of the honey bees ingesting the compositions of Comparative Examples 1 and 2 were not substantially increased.

Test Example  3. Experiments to increase the number of bees

The feeds containing supplementary feed for bees prepared in Examples and Comparative Examples were fed to 100 bees from the 15th day until three months after the feeding of queen bees and the bees And the average number of individuals was shown in Table 3 below.

division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Population 118 125 131 127 84 80

As shown in Table 3, although the number of bees after feeding for 15 months from the feed containing the supplementary feed composition prepared according to Examples 1 to 4 of the present invention significantly increased after 3 months, Comparative Examples 1 and 2 It was confirmed that the number of dead bees decreased and the number of the bees decreased.

In addition, although the color of the larvae of the queen bees fed with the feed containing the supplementary feed composition prepared in Examples 1 to 4 of the present invention was yellow, Of the queen bees fed diets containing supplementary feed composition were brown.

The yellowing of the stool plate means that the state of the larvae is good, and the thing that is brown means that the state of the larvae is not good.

Test Example 4. Increase in Honey Production

The feeds containing the supplementary feed for bees prepared in Examples and Comparative Examples were fed to 100 bees and the yield of honey was measured after 3 months. The results are shown in Table 4 below.

division Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Total feed production (kg) 10.0 9.9 10.1 9.9 9.9 10.1 Production after feeding (kg) 11.4 12.1 13.0 12.7 8.5 8.3

As shown in the above Table 3, the feedstuff containing the supplementary feed composition prepared according to Examples 1 to 4 of the present invention showed an increase of about 10 to 30% in honey production after feeding for 3 months, 2 showed a decrease in honey production due to the decrease of population.

Therefore, it can be seen that the increase in the health status and population of bees is directly related to the increase of honey production.

Claims (12)

(a) mixing the rice bran and water at a weight ratio of 1: 0.05 to 0.3;
(b) mixing molasses and at least one bacterium selected from the group consisting of Bacillus sp. , Saccharomyces sp. , Lactobacillus sp. and Streptomyces sp . ;
(c) mixing molasses and water mixed with the bacteria at a weight ratio of 1: 0.1 to 0.5;
(d) adding the molasses mixture prepared in step (c) to the rice gruel produced in step (a), stirring the mixture in a rotary fermenter set at 35 to 38 ° C for 5 to 30 minutes, &Lt; / RTI &gt; and fermenting for 36 to 54 hours to produce a culture; And
(e) drying the culture. &lt; Desc / Clms Page number 20 &gt; The method according to claim 1, wherein the rice bran is steamed at 100 to 150 ° C in step (a). The method according to claim 1, wherein the squid cartilage powder is added in step (a). The method according to claim 3, wherein the content of squid cartilage powder is 20 to 50 parts by weight based on 100 parts by weight of rice bran. [6] The method of claim 1, wherein the content of the molasses in the step (b) is 30 to 60 parts by weight based on 100 parts by weight of the rice bran. The method according to claim 1, wherein the saccharide is added as a carbon source in the step (b). 7. The method according to claim 6, wherein the scavenging soil is prepared by suspending the scavenging soil in water to a concentration of 2 to 30%, stirring the slurry at 60 to 100 DEG C for 30 minutes to 2 hours while adjusting the pH of the suspension to 6 to 9 &Lt; / RTI &gt; wherein the method comprises the steps of: [6] The method of claim 1, wherein in step (d), the mixture of rice bran and molasses is mixed at a weight ratio of 1: 0.02 to 0.1. The method according to claim 1, wherein the egg white peptide is added during fermentation in step (d). [Claim 11] The method of claim 9, wherein the content of the egg white peptide is 5 to 20 parts by weight based on 100 parts by weight of rice bran. 2. The method according to claim 1, wherein in the step (e), a Bacillus subtilis My process by Sergio subtilis (KCTC 3239)) and non-saccharide Jia (Saccharomyces cerevisiae (KCTC 7915)) is inoculated and dried for 48 hours. delete