KR102210145B1 - Method for preparing calcium-fortified heat-resistant feed additive and feed additive prepared thereby - Google Patents

Abstract

The present invention relates to a calcium-enhanced heat-resistant feed additive and a method for manufacturing the same, and more particularly, to a feed with a function of promoting growth through reduction of mortality rate due to increase of immunity of livestock and improvement of meat quality and digestion activity by adding calcium It relates to additives and methods of preparing them.
The present invention is after culturing microorganisms including Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae, microorganisms including molasses and water , Microbial culture solution preparation step of filtering a liquid substance in the pH range of 3.5 to 4.5; Fermenting the microbial culture liquid at a temperature of 35 to 45° C. to prepare a microbial culture liquid with enhanced heat resistance; Preparing a microbial probiotic by adding rice bran, ox flour, and molasses to the heat-resistance-enhanced microbial culture broth, mixing and fermentation culture; Hot-air drying the microbial probiotic to prepare a powder probiotic; And 100 parts by weight of the powdered probiotic, and 0.1 to 0.5 parts by weight of the shell powder are added based on 100 parts by weight of the powdered probiotic, and at the same time as stirring, drying and sterilizing the shell at 80 to 120°C, to prepare a calcium-enhanced powder probiotic Step; characterized in that it comprises, calcium-enhanced heat-resistant feed additive and a method of manufacturing the same as the technical gist.

Description

Method for preparing calcium-fortified heat-resistant feed additive and feed additive prepared thereby {Method for preparing calcium-fortified heat-resistant feed additive and feed additive prepared thereby}

The present invention relates to a method of manufacturing a calcium-enhanced heat-resistant feed additive, and to a feed additive prepared thereby, and specifically, a method of manufacturing a calcium-enhanced heat-resistant feed additive using a microbial culture solution and shell powder with enhanced heat resistance, and manufactured thereby It relates to feed additives.

The most important factor in the livestock industry is resistance to various diseases and promotion of growth through nutrients.

Among them, antibiotics can be used as a substitute for antibiotics as the preparation and addition of antibiotics as feed additives has been prohibited in Korea since 2011, and the production and use of microbial and functional probiotics using microorganisms such as probiotics is increasing significantly. to be. In addition, excellent microorganisms are being developed as essential agents for strengthening human immunity and are being developed for various uses in livestock and agriculture.

In particular, in the case of microorganisms used in the livestock field, in general, it helps to promote growth by enhancing the digestive power of the feed consumed through the improvement of resistance and treatment of intestinal abnormalities and the activity of microorganisms in the body. It helps to improve the environment of livestock and improve the productivity of livestock by suppressing odor and hydrogen sulfide (NH ₃ ) generation in the farm.

However, commercially available products are not as effective as conventional antibiotics, and this is because they are easily killed at a temperature of 35°C or higher due to the nature of microorganisms, and are unable to adapt to various environments and mutations occur.

On the other hand, the content of calcium and phosphorus in grains, their by-products, and grasses, etc., which are used as main feed ingredients, is lower than that of animal feed. In particular, calcium is a nutrient that maintains healthy bones and teeth of livestock and regulates muscle contraction and cell division. Especially, in pregnant and lactating livestock, a large amount of calcium is required through fetal development and fermentation. As it is one, the demand is on the rise.

However, grains and grasses grown in the same acidic soil conditions as in Korea, especially low-quality forage, are largely lacking in calcium and phosphorus, so livestock may suffer from deficiency symptoms, so it is necessary to supplement calcium through other factors.

In this regard, representative raw materials for calcium include shells, egg shells, and bones of livestock. Among these, in the case of shells, if the size of the pulverized particles is large when used in feed for livestock, not only does it affect indigestion, but also causes component deformation when heated directly, and may cause problems with storage properties.

In addition, as the production capacity of livestock according to the nutrients of feed has been improved recently, the demand for heat-resistant feed additives with enhanced nutrients is increasing, and technology development to solve this problem is urgently required.

Korean Patent Publication No. 10-2010-0137887

The present invention was invented to solve the above problems, and by using pulverized shell powder and maximizing heat resistance, the feed additive does not die early after being absorbed into the body of livestock, and the unique intestinal activity of the microbial agent is enhanced. The challenge is to provide a method of manufacturing a calcium-enhanced heat-resistant feed additive that not only improves immunity and digestive activity, but also allows long-term storage.

In addition, the present invention is a solution to provide a calcium-enhanced heat-resistant feed additive prepared by the above method.

In order to solve the above problems, the present invention,

0.5 to 1 parts by weight of microorganisms including Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae based on 100 parts by weight of water and 100 parts by weight of the water, and After culturing a mixture containing microorganisms containing 4 to 6 parts by weight of molasses, a microbial culture solution preparation step of filtering a liquid substance having a pH of 3.5 to 4.5;

Fermenting the microbial culture liquid at a temperature of 35 to 45° C. to prepare a microbial culture liquid with enhanced heat resistance;

Adding 2 to 5 parts by weight of rice bran, 0.5 to 2 parts by weight of ox flour, and 1 to 3 parts by weight of molasses to 100 parts by weight of the heat-resistance-enhanced microbial culture solution, mixing and fermenting to prepare a microbial probiotic;

Hot-air drying the microbial probiotic to prepare a powder probiotic; And

Characterized in that it comprises; adding 0.1 to 0.5 parts by weight of shell powder based on 100 parts by weight of the prepared powder probiotic, drying and sterilizing at 80 to 120°C while stirring, to prepare a calcium-enhanced powder probiotic; , The manufacturing method of calcium-enhanced heat-resistant feed additive and the feed additive manufactured thereby are the technical gist.

Preferably, the shell powder is pulverized into a particle size of 50 to 200 μm, and then foreign substances are removed through a filter with 50 to 100 mesh, and for 4 to 5 minutes at a temperature of 110 to 120°C in a sterilizer. It is characterized by being sterilized.

Preferably, in the microbial culture production step, after culturing a mixture containing microorganisms including 0.5 to 1 parts by weight of microorganisms and 4 to 6 parts by weight of molasses in 100 parts by weight of water, the liquid substance is filtered, and the heat resistance-enhanced microorganisms In the step of preparing the culture medium, the microbial culture medium is fermented and cultured at a temperature of 35 to 45° C. for 45 to 50 hours.

Preferably, in the step of preparing the calcium-enhanced powder probiotic, the shell is dried and sterilized for 6 to 8 hours at a temperature of 80 to 120°C and a humidity of 15 to 18%.

In order to solve the above other problems, the present invention also makes a technical gist of the calcium-enhanced heat-resistant feed additive, characterized in that it is manufactured by the above-described manufacturing method.

As described above, according to the manufacturing method of the present invention, by enhancing the heat resistance of microorganisms through the step of preparing a microbial culture solution with enhanced heat resistance and a step of preparing a microorganism probiotic, a heat-resistant feed additive that excellent bacteria do not kill even at 40°C You can do it. And by adding the sterilized and pulverized shell powder to the heat-resistant feed additive, it is possible to prepare an additive with enhanced calcium.

In addition, the feed additive prepared according to the present invention has the effect of strengthening calcium, strengthening bones and teeth of livestock, controlling muscle contraction and cell division, and solving the symptoms of calcium deficiency in livestock during pregnancy. In addition, mortality is significantly reduced due to increased immunity due to enhanced heat resistance. Compared to conventional meat, it can exhibit excellent effects in improving meat quality and removing odors. It is prevented from spoiling even at high temperatures, so it has a shelf life of 6 months to 1 year. , It has excellent safety effect by maintaining organic composition without mutation in various environments.

1 is a flow chart showing a method of manufacturing a calcium-enhanced heat-resistant feed additive of the present invention.
Figure 2 is a flow chart showing a method for producing a shell powder additive of the present invention.
Figure 3 is a feed verification certificate of the calcium-enhanced heat-resistant feed additive of the present invention.

Hereinafter, the present invention will be described in detail with reference to the drawings.

1 is a flow chart showing a method of manufacturing a calcium-enhanced heat-resistant feed additive of the present invention. Referring to this, the calcium-enhanced heat-resistant feed additive of the present invention is a microbial culture solution manufacturing step (S10), a heat resistance enhanced microorganism culture solution manufacturing step (S20). ), a microbial probiotic manufacturing step (S30), a powder probiotic manufacturing step (S40), and a calcium-enhanced powder probiotic manufacturing step (S50).

First, the microbial culture solution preparation step (S10) with respect to 100 parts by weight of water and 100 parts by weight of water is Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae. After culturing a mixture containing microorganisms including 0.5 to 1 parts by weight of microorganisms and 4 to 6 parts by weight of molasses, a liquid substance having a pH in the range of 3.5 to 4.5 is filtered and separated into a microorganism culture solution.

Prior to this, I will describe the microorganisms applied in the microbial culture solution manufacturing step.

First, Bacillus subtilis is called Bacillus subtilis (a type of aerobic bacteria, non-toxic, and spore-forming bacteria), and is a representative type of bacteria belonging to the genus Bacillus, widely distributed in soil, hay, and dust. It is an aerobic microorganism.

These Bacillus subtilis produce proteolytic enzymes, increase beneficial bacteria in the intestine, reduce odor and pests, and are excellent in feed efficiency and weight gain, so that healthy growth of livestock can be achieved.

Second, Lactobacillus plantarum is a salt-resistant lactic acid bacteria (bacteria, lactic acid bacteria that produce lactic acid by decomposing sugars such as glucose), and corresponds to aerobic anaerobicity.

In other words, Lactobacillus plantarum produces organic acids as a by-product, prevents bacterial enteritis, inhibits the growth of harmful bacteria in the body, or keeps the intestines clean by inhibiting the growth of harmful bacteria in the intestine and other bacteria from outside through food. It serves to prevent diarrhea and constipation. It also plays a role in removing odor by suppressing the generation of ammonia and hydrogen sulfide present in livestock manure.

Third, Saccharomyces cerevisiae is a representative yeast belonging to associa fungi (a fungus that forms associa spores by sexual reproduction among fungi), and is a yeast used in brewing including baker's yeast and brewer's yeast. Most of these can be said to be related to this species.

In particular, Saccharomyces cerevisiae supplies useful physiologically active substances such as amino acids, vitamins, minerals and digestive enzymes, and improves the productivity of livestock by enhancing the metabolic activity of yeast itself. In addition, by promoting fermentation, it promotes the decomposition of organic matter using microorganisms and improves palatability.

On the other hand, if these microorganisms are less than 0.5 parts by weight, the microorganisms required for cultivation are not sufficiently supplied, so that the number of microorganisms does not effectively proliferate, thereby causing a problem of reducing the number of beneficial bacteria of the feed additive. On the other hand, when the amount of microorganisms exceeds 1 part by weight, contamination of the microorganism culture solution may occur because the microorganisms grow through sufficient feeding activities. Therefore, the microorganism is preferably added in the range of 0.5 to 1 parts by weight based on 100 parts by weight of water.

At this time, it is preferable that 4 to 6 parts by weight of molasses are additionally added based on 100 parts by weight of water. The molasses is a thick syrup that is incidental when sugar cane or sugar beets are processed into sugar, and generally contains 20 to 30% of water, and the main component is sugar, and various nutrients such as dietary fiber, folic acid, protein, vitamins and potassium. As a material having, in the present invention, it is added to be used as food for cultivation of microorganisms.

More preferably, the molasses is added to 4 to 6 parts by weight based on 100 parts by weight of water. If it is added in less than 4 parts by weight, the growth efficiency of microorganisms is deteriorated because it is not sufficiently supplied as food for the culture of microorganisms, and if it is added in more than 6 parts by weight, the space in which microorganisms in the microbial culture solution live or propagate becomes insufficient, which also reduces the growth efficiency of microorganisms. This is because there is a problem that the product properties are deteriorated due to the decrease and the viscosity of the microbial culture solution is too high.

After culturing the microorganism in this way, the remaining microorganism culture solution can be obtained by filtering the liquid substance in the pH range of 3.5 to 4.5. In other words, it is desirable to remove the remaining molasses and residual by-products in the liquid substance in the pH range of 3.5 to 4.5, which deteriorates the feed additive.

Next, the heat resistance-enhanced microbial culture solution manufacturing step (S20) is a fermentation-culture for 45-50 hours at 35-45°C where common microorganisms cannot survive the microbial culture solution mixed with molasses supplied as food to enhance heat resistance. This is the step of preparing the microbial culture solution.

In detail, it is good to ferment culture while stirring for 45-50 hours at 35-45℃. If fermentation culture is less than 35℃ or less than 45 hours, unfermented residual material is generated, which does not extend the preservation period of the final product. , It is insignificant to impart heat resistance to the strain, and if the fermentation culture exceeds 45°C or exceeds 50 hours, excellent strains may be killed before the microbial probiotic production step, so 45 to 50 hours under 35 to 45°C. It is preferable to go through the heat resistance strengthening process while stirring. As a result of the experiment, it could be confirmed that this process is the most optimal condition for fermentation culture at 40°C for 48 hours.

Here, the general strain is fermented and cultured under 25 to 35°C, which is very important because the strains start to die when it exceeds 40°C, and most die when it exceeds 80°C.

Subsequently, in the microbial probiotic manufacturing step (S30), 2 to 5 parts by weight of purified rice bran, 0.5 to 2 parts by weight of Oktae powder, and 1 to 3 parts by weight of molasses are added to 100 parts by weight of the heat resistance-enhanced microbial culture solution and mixed, and 45 to 50 It is a step of fermenting and culturing for a period of time to prepare a microbial probiotic.

Rice bran is a flesh bran composed of embryos, fruit skin, and algal layer. It contains a lot of minerals such as vitamins B1, B2, niacin, phosphorus, potassium, calcium, and fiber, and contains 15 to 21% of fat such as γ-orizanol. It was applied to the present invention because it is contained and rich in nutrition.

If less than 2 parts by weight of rice bran is added to 100 parts by weight of the microbial culture, the efficiency decreases as a feed additive, and if it is added in excess of 5 parts by weight, it becomes more viscous and becomes entangled, making it difficult to mix with the feed. Since feeding is not easy, rice bran is preferably added in the range of 2 to 5 parts by weight.

Oktae powder is a powder of fine particles and granules by breaking the outer shell and embryo of corn grains with a lot of carbohydrates. It is rich in essential fatty acids linoleic acid and dietary fiber, and nutrients such as beta-carotene, potassium, calcium, zinc, iron, niacin, and vitamin oil. Was applied to the present invention.

At this time, since rice bran alone is insufficient in the subsequent fermentation culture, 0.5 to 2 parts by weight of ox powder is additionally added to 100 parts by weight of the microbial culture so that the fermentation culture is made more efficient.

As a result of the experiment, if the jade flour is less than 0.5 parts by weight, it is insignificant to assist the role of the rice bran, and if it exceeds 2 parts by weight, the miscibility with the rice bran is not good, so it is preferably added in the range of 0.5 to 2 parts by weight.

On the other hand, the addition of molasses further enhances the performance of the product because molasses is added in the previously described microbial culture solution manufacturing step to complete the liquid microbial culture, but in the process of powdering it again, the growth of microorganisms is more active. An additional 1 to 3 parts by weight of molasses is mixed with respect to 100 parts by weight of microorganisms.

Here, if the additional molasses is added in an amount of less than 1 part by weight, it may not play an auxiliary role in the growth of microorganisms, and if it exceeds 3 parts by weight, the microorganism may be overgrown and deteriorate during the heat resistance enhancement process.

As can be seen here, it is preferable to add the additional molasses relatively less than the initial molasses input amount.This is because if the additional molasses is added relatively more than the initial molasses, the amount of added molasses increases and the viscosity is too high. This is because the castle is significantly lowered.

Next, the powder probiotic manufacturing step (S40) is a step of preparing a powder probiotic by hot air drying the microorganism probiotic.

This is to assist in maintaining long-term storage of feed additives, and conventional feed additives cannot preserve moisture and are oxidized within a short period of time, or plastic or paper packaging materials such as polyethylene that are easy to contact with air are often used. Or, if you look at the moisture content, the strains are killed.

In this case, it is preferable to dry with hot air for 40 to 48 hours at a temperature of 90 to 100°C so that the water content of the probiotic powder is 12 to 14%.

If hot air drying so that the moisture content is less than 12%, the function of the feed additive will not function properly. On the other hand, if the moisture content exceeds 14%, additional fermentation proceeds during the storage process, causing spoilage easily.

Finally, in the calcium-enhanced powder probiotic manufacturing step (S50), 100 parts by weight of the powder probiotic and 0.1 to 0.5 parts by weight of the shell powder are added based on 100 parts by weight of the powder probiotic, and the shell is dried at 80 to 120°C while stirring. And sterilizing, producing a calcium-enhanced powder probiotic.

In detail, the shell powder used in the manufacturing step of the calcium-enhanced powder probiotics is oyster (petrol), scallops, clams (reformed clams), dongjuk clams, cockles, ramie clams (autumn clams), marinated clams, clams, clams, sackcloth, abalone, clams Use dried shells of at least one of, cockle or mussel.

2 is a flow chart showing the shell powder production process of the present invention. If the shell is washed and dried, and then used by crushing the shell into coarse particles in the pulverization process, it is not suitable for livestock such as cattle and pigs to absorb nutrients, and it can be dangerous. need. Therefore, in the case of the shell powder used in the present invention, after crushing to a particle size of 50 to 200 μm, foreign substances are removed through a filter with 50 to 100 mesh, and sterilized for 4 to 5 minutes at a temperature of 110 to 120°C in a sterilizer. Was applied.

Here, when the shell powder is directly heated or fired, the composition may change, so care must be taken in the process.

On the other hand, it is irrelevant to substitute the powdered shell powder after sterilization instead of the process of directly pulverizing the shell powder, but a process of confirming the particle size and powder state of the powder is essential.

In addition, the addition ratio of the shell powder was applied not more than 0.5 parts by weight, because when the mixture ratio of the shell powder exceeds 0.5% parts by weight, the inherent effect of the probiotic is irrelevant, and the fragrance of the shell affects the unique flavor. .

Hereinafter, the present invention will be described in detail by way of examples.

<Example 1> Preparation of heat-resistant feed additive

First, a microorganism containing 0.5 parts by weight of microorganisms including Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae and 100 parts by weight of water and 4 parts by weight of molasses The mixture containing this was cultured, and the liquid material was filtered in a pH range of 4, and this was fermented and cultured in an environment at a temperature of 40°C.

To 100 parts by weight of the culture broth, 3 parts by weight of rice bran, 1 part by weight of ox powder, and 2 parts by weight of molasses were added and mixed, followed by fermentation and culture, followed by drying with hot air to powder.

<Example 2> Calcium-fortified heat-resistant feed additive manufacturing

First, a microorganism containing 0.5 parts by weight of microorganisms including Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae and 100 parts by weight of water and 4 parts by weight of molasses The mixture containing this was cultured, and the liquid material was filtered in a pH range of 4, and this was fermented and cultured in an environment at a temperature of 40°C.

To 100 parts by weight of the culture broth, 3 parts by weight of rice bran, 1 part by weight of ox powder, and 2 parts by weight of molasses were added and mixed, followed by fermentation culture, followed by hot air drying and powdered, and 0.3 parts by weight of shell powder was added based on 100 parts by weight of the prepared powder. And through a stirring process to prepare a calcium heat-resistant feed additive.

At this time, the shell powder was prepared by drying and sterilizing treatment for 7 hours at a temperature of 100°C and 18% humidity.

For the prepared feed additive, a feed verification test was requested to the Korea Sweet Feed Association. As shown in FIG. 3, the results were analyzed for calcium 7.46%, Bacillus subtilis 1.4×10 ⁶ (cfu/g) Lactobacillus plantarum 1.1×10 ⁷ (cfu/g) and Saccharomyces cerevisiae 4.8×10 ⁷ (cfu/g). .

<Experimental Example 1> Results of increasing heat-resistant feed additive

Pig farms located in the so-called-gun, Gyeongsangbuk-do were selected, and the heat-resistant feed additive prepared in Example 1 was fed 0.2% of the daily feed amount for 8 months. The results are summarized in Tables 3 to 5, from which the conclusions shown in Table 2 could be obtained.

'A' farmhouse Shipment was advanced by 10 to 15 days and weight gained.
Digestion efficiency and weight gain rate improved by more than 10%. 'B' farmhouse Usually, about 64 teas (6 tons) of feed come in, but with 55 teas, the amount of feed declined.
With an average shipment weight of 118 to 120 kg, feed efficiency improved by 15%.

Table 3 below shows the trend and shipment status of the number of pigs reared in control pig farms. Table 4 shows the trend and shipment status of the number of pigs reared in the experimental zone. In addition, Table 5 can be seen that, based on the number of days of fattening of the control, the average increase was 1.5, and the experimental group was increased by 12%.

Date Base head Number of standing Number of shipments Money man
Number of breeding heads Number of shipments
weight Shipping grade Fattening day
exchange Fattening days
standard
Daily increase Total weight Average weight A B C D E Stocking day Fattening
Number of days August
10 days 232 115 - 347 August
30 days 347 65 - 412 September
6 days 412 66 - 478 September
28 days 478 83 - 561 October
23 days 561 - 60 501 6830 113.83 31 18 7 4 August
10 days 74 1.54 November
6 days 501 - 60 441 7185 119.75 32 18 4 6 August
10 days 88 1.36 November
13th 441 - 60 381 7135 118.92 30 21 7 2 August
30 days 75 1.59 November
20 days 381 - 60 321 6870 114.50 24 16 11 9 September
6 days 75 1.53 Shipping grade
ratio 49% 30% 12% 9% 0% Fattening days
Standard daily wage
Gain average 1.50

Date Foundation
Head Standing
Head Shipment
Head Money man
Number of breeding heads Number of shipments
weight Shipping grade Fattening day
exchange Fattening days
standard
Daily weight gun
weight Average
weight A B C D E Stocking day Fattening
Number of days In July
6 days 199 79 - 278 In July
14 days 278 80 - 358 In July
18th 358 72 - 430 In July
28 days 430 86 - 516 August
19th 516 35 - 551 August
28 days 551 - 35 516 In July
6 days September
11th 516 - 35 481 In July
6 days September
18th 481 - 70 411 8055 115.07 31 18 15 6 In July
14 days 66 1.74 September
28 days 411 - 70 341 8540 122.00 28 24 7 11 In July
18th 72 1.69 October
5 days 341 80 - 421 October
10 days 421 - 70 351 8310 118.71 42 21 4 3 In July
28 days 74 1.60 October
13th 351 83 - 434 October
26th 434 79 - 513 October
31 days 513 - 38 475 4600 121.05 19 9 3 7 August
19th 73 1.66 Shipping grade
ratio 48% 29% 12% 11% 0% Fattening days
Standard daily wage
Gain average 1.68

division Daily increase ratio(%) Control 1.50 100 Experiment 1.68 112

From the results of Tables 3 and 4, the feed additive of the present invention has enhanced heat resistance, prevents spoilage even at high temperatures, has an effect of having a shelf life of 6 months to 1 year, and has excellent storage stability in various environments. I could confirm that.

From the above results, it can be seen that according to the present invention, it is possible to prepare a feed additive capable of enhancing storage stability by helping to increase the immunity of livestock by enhancing heat resistance and strengthening calcium, and increasing the shelf life.

As the specific parts of the present invention have been described in detail above, various modifications and variations will be possible for those of ordinary skill in the art without departing from the intrinsic characteristics of the present invention. Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but are intended to be described, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (5)

0.5 to 1 parts by weight of microorganisms including Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae based on 100 parts by weight of water and 100 parts by weight of the water, and After fermentation culture by adding 4-6 parts by weight of molasses, a microbial culture solution manufacturing step of filtering a liquid substance in a pH range of 3.5 to 4.5;
Fermenting the microbial culture liquid at a temperature of 35 to 45° C. to prepare a microbial culture liquid with enhanced heat resistance;
Adding 2 to 5 parts by weight of rice bran, 0.5 to 2 parts by weight of ox flour, and 1 to 3 parts by weight of molasses to 100 parts by weight of the heat-resistance-enhanced microbial culture solution, mixing and fermenting to prepare a microbial probiotic;
Hot-air drying the microbial probiotic to prepare a powder probiotic; And
Including; adding 0.1 to 0.5 parts by weight of shell powder based on 100 parts by weight of the prepared powder probiotic, drying and sterilizing at 80 to 120° C. while stirring, to prepare a calcium-enhanced powder probiotic, and
In the heat resistance enhanced microbial culture solution manufacturing step,
The microbial culture medium is fermented and cultured for 45 to 50 hours in an environment of 35 to 45°C,
Calcium, characterized in that the Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae contained in the calcium-enhanced powder probiotic do not die even at 80°C Method of manufacturing enhanced heat-resistant feed additive The method of claim 1,
The shell powder is obtained by pulverizing the dried shell into a particle size of 50 to 200 μm, removing foreign substances through a filter with 50 to 100 mesh, and sterilizing for 4 to 5 minutes at a temperature of 110 to 120°C in a sterilizer. Characterized in, the method for producing a calcium-enhanced heat-resistant feed additive. delete The method of claim 1,
In the step of preparing the calcium-enhanced powder probiotic, the shell is prepared by drying and sterilizing the shell for 6 to 8 hours at a temperature of 80 to 120°C and a humidity of 15 to 18%. Method for producing additives. Claims 1, 3, and 4, characterized in that produced by the manufacturing method of any one of claims, calcium-enhanced heat-resistant feed additive.

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