KR20160091611A - Method for preparing fermented extract of Oyster Shell by using of microorganism - Google Patents

Abstract

The present invention relates to a method for producing a fermented product of oystershells using microbes. To this end, the method comprises the following steps: a first step of washing oystershells and drying the same thereafter; a second step of processing the dried oystershells into powder by pulverization and then sterilizing the same; a third step of producing fermentation microbes using a bioreactor; a fourth step of mixing the powder in the second step with the fermentation mibrobes and adding a nitrogen source so as to produce a primary fermented product; a fifth step of sterilizing and drying the primary fermented product; a sixth step of mixing the primary fermented product in the fifth step with the fermentation microbe and adding the nitrogen source so as to produce a secondary fermented product; a seventh steps of sterilizing and drying the secondary fermented product in the sixth step; an eighth step of mixing the secondary fermented product in the seventh step with fermentation microbe and adding the nitrogen source so as to produce a tertiary fermented product; ninth step of sterilizing and drying the fermentation microbe and the tertiary fermented product in the eighth step; and a tenth step of producing a fermented product by pulverizing the fermented product in the ninth step.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a fermented oyster shell fermented by using microorganisms,

The present invention relates to a method for producing oyster shell fermentation products through a multiple fermentation bioprocess using microorganisms, and more particularly, to a method for producing oyster shell fermentation products using a fermentation microorganism, Fermentation products obtained through fermentation can be modified to various forms such as feed additives for livestock, raw materials for fermentation organic fertilizer for agriculture including fermented feed additives, and microorganisms capable of mass production of high-cost fermented products at low cost The present invention relates to a method for producing an oyster shell fermentation product.

Usually, about 500,000 tons of oyster shells are produced annually from the oyster farming industry in the south coast, but their use is limited, and the oyster shells are being processed in a way that they are buried on the shore or buried in the sea. In the case of landfill, it causes various environmental problems such as coastal pollution and damage to natural scenery. Secondary environmental pollution caused by landfill and marine pollution by securing landfill site or leachate is becoming a problem in landfilling. Therefore, the government has been promoting policies to recycle oyster shell waste, and many researchers have been conducting extensive research on the recycling of fossil fuels.

On the other hand, calcium contained in the oyster shell is one of the inorganic salts necessary for livestock, 99% is contained in the bones of the livestock, and 1% is involved in the physiology of the livestock. Lack of calcium slows and weakens the bone growth rate. There is a calcium-based shell recycle feed that improves productivity by providing a full complement of essential nutrients with a small amount of this formulation.

As a prior art related thereto, Korean Patent Laid-Open No. 10-2010-0037296 entitled " Method and Apparatus for Producing High-purity Calcium Powder Using Shellfish ", Korean Patent Registration No. 10-0993223 " , Korean Patent Registration No. 10-1328671 entitled " Method for producing a calcium-reinforced livestock feed additive including crustacean and shell, and calcium-supplemented livestock feed additive obtained therefrom, and livestock feed containing the same " .

However, the above-mentioned prior art document does not disclose that there is no patent registration and publication document on the oyster shell fermentation product and the microbial secondary metabolite composition after the fermentation process using the fermentation microorganism on the oyster shell, and in particular, In case of ingestion of livestock due to salt remaining in the shell, digestive disturbance is caused, so absorption power is lowered, resulting in poor growth and development, and the quality of livestock is lowered.

Korean Patent Laid-Open No. 10-2010-0037296 "Method and apparatus for manufacturing high-purity calcium powder using shellfish" (Published date: Apr. 09, 2010) Korean Registered Patent No. 10-0993223 entitled " Method for manufacturing shell recycled feed "(registered on Nov. 03, 2010) Korean Patent Registration No. 10-1328671 entitled " Method for producing calcium-fortified livestock feed additive including crustacean and shell, and calcium-supplemented livestock feed additive obtained therefrom and livestock feed containing the same " 11. 06)

The object of the present invention to fundamentally improve the above problems is to obtain a fermented product through a bioprocess through multiple fermentation over 1, 2, and 3 stages using an oyster shell by using a fermenting microorganism, The present invention provides a method for producing an oyster shell fermentation product using a microorganism capable of mass production of a substance capable of changing formulations from a variety of raw materials including agricultural animal organic fertilizer raw materials including livestock feed additives, have.

In order to accomplish the above object, the present invention provides a method for treating oysters, comprising: a first step of washing and drying oyster shells as a raw material; A second step of crushing the dried material of the first step to produce powder, and sterilizing the bacterium using a rapid sterilization apparatus (Autoclave); A third step of culturing the fermentation microorganism using the bioreactor in the second step; A fourth step of mixing the powder of the second step with a fermenting microorganism using an incubator, adding a nitrogen source necessary for fermentation to produce a first fermentation product through a first biological reaction; A fifth step of sterilizing and drying the fermenting microorganism using a rapid sterilization apparatus (Autoclave) in the fourth step of fermentation; A sixth step of mixing the primary fermentation product with the fermentation microorganism using an incubator and adding a nitrogen source necessary for fermentation to produce a secondary fermentation product through a secondary biological reaction; A seventh step of sterilizing and drying the fermentation microorganism using a rapid sterilization apparatus (Autoclave) in the sixth fermentation step; An eighth step of mixing the secondary fermentation product of step 7 with a fermenting microorganism using an incubator, adding a nitrogen source necessary for fermentation to produce a tertiary fermentation product through a tertiary bioreaction; A ninth step of drying the third fermentation product of step 8 at a moisture content of 18% using a drier; And a 10th step of pulverizing the fermented product of step 9 with a powder of 60 to 80 mesh using a pulverizer to produce a fermented product.

According to the present invention, in the second step, the oyster shell dried product is formed into a powder having a size of 0.5 to 1 cm with a crusher, and sterilized by using a rapid sterilization apparatus at a temperature of 100 to 130 ° C for 15 to 25 minutes .

In addition, according to the present invention, the third step is a step wherein the fermenting microorganisms Saccharomyces cerevisias, Bacillus natto, Aspergillus oryzea, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lentinula edodes, Phellinus linteus, Cordyceps militaris, Pleurotus ostreatus , Trametes versicolor is cultivated so that the number of populations per ml of culture is 10 ^{+ 1} to 10 ^{+ 7} , singly or mixed.

According to the present invention, the fermentation microorganisms are mixed in the fourth, sixth, and eighth steps, and 30 to 70% by weight of the nitrogen source is mixed with the incubator at a temperature of 25 to 40 DEG C and a humidity of 50 to 80% And fermenting the fermented product for 2 to 8 days under the condition of keeping the fermented product.

According to the present invention, in the fifth and seventh steps, the fermented material is sterilized using a rapid autoclave at a temperature of 120 ° C. to 130 ° C. for 15 to 30 minutes during the biological reaction, .

It should be understood, however, that the terminology or words of the present specification and claims should not be construed in an ordinary sense or in a dictionary, and that the inventors shall not be limited to the concept of a term It should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention based on the principle that it can be properly defined. Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention, and not all of the technical ideas of the present invention are described. Therefore, It is to be understood that equivalents and modifications are possible.

As described in the above constitution and operation, the oyster shell fermentation product obtained through the manufacturing method according to the present invention can obtain the fermentation product through the bioprocessing through the multiple fermentation over 1, 2, and 3 stages using the oyster shell by using the fermenting microorganism , It is possible to mass-produce mass-produced substances which can be formulated into various raw materials such as feed additives for livestock, raw materials for organic fertilizer for agriculture, and additives at low cost.

FIG. 1 is a block diagram sequentially showing a method for producing an oyster shell fermentation product by microbial fermentation according to the present invention. FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention relates to a method for producing an oyster shell fermentation product using an oyster shell as a raw material and briefly describes washing, drying, crushing, sterilization, fermenting microorganism culture, primary fermentation, sterilization, secondary fermentation, sterilization, The fermentation product is prepared through the fermentation, drying and pulverization steps.

In the first step of the present invention, the oyster shell is washed with a raw material and dried. The first step is a step of washing and drying the raw material, washing and drying the oyster shell as a raw material. It cleans the oyster shells in water, dries the remaining water at room temperature during washing, and introduces an automatic device to improve quality stability and productivity. Here, in this first step, the drying process provides the surface moisture of the washed oyster shell to dry.

In the second step of the present invention, the dried material of the first step is crushed to produce powder, and the bacteria are sterilized by using a rapid autoclave. The second step is a step of crushing and sterilizing the raw material (oyster shell) which has been subjected to the washing and drying steps. In particular, it is preferable to introduce a dedicated automation facility to improve the productivity in crushing the raw material to produce powder.

At this time, in the second step according to the present invention, the oyster shell dried material is produced as a powder of 0.5-1 cm with a crusher, and sterilized by using a rapid sterilization apparatus at a temperature of 100-130 ° C for 15-25 minutes. This produces dried material, that is, oyster shells, as a powder of 0.5 to 1 cm using a crusher. Next, for sterilization of the microorganisms contained in the powder, sterilization is carried out at a temperature of 100 to 130 ° C for 15 to 25 minutes using an autoclave, which is a high-pressure sterilization apparatus, to remove the germs.

The third step of the present invention is produced by culturing a fermenting microorganism using a bioreactor. The third step is the step of producing the fermenting microorganisms necessary for the biological reaction. In order to cultivate the fermenting microorganisms, it is preferable to introduce the dedicated automatic facilities to improve the quality stability and productivity.

At this time, the third step according to the present invention is characterized in that the fermenting microorganisms Saccharomyces cerevisiae, Bacillus natto, Aspergillus oryzea, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lentinula edodes, Phellinus linteus, Cordyceps militaris, Pleurotus ostreatus , Trametes versicolor is cultured to a population of 10 ⁺¹ to 10 ⁺⁷ per ml of culture, and these are cultured singly or mixed. In order to ferment the rapidly sterilized oyster shell powder, soil microorganisms, mushroom strains, lactic acid bacteria, and fungi, which have proven useful among the fermenting microorganisms, are collected on a bioreactor in a population of 10 ⁺¹ to 10 ⁺⁷ per ml on a bioreactor Cultivated in large quantities as much as possible, and then fermented microorganisms are produced singly or in combination.

Among the fermenting microorganisms to be used in the present invention, Saccharomyces cerevisias ( Saccharomyces cerevisiae ), Bacillus natto ( Bacillus natto ), Aspergillus oryzea (koji mold) and Lactobacillus acidophilus ( Lactobacillus acidophilus ) And Lactobacillus bulgaricus (Lactobacillus bulgaricus ) are recognized as the most important microbes in industry, especially due to the variety of chemical structures and abundance of species in secondary metabolites. Metabolites are classified into primary metabolites and secondary metabolites. Primary metabolites are substances that play a fundamental role in living organisms such as processed amino acids, saccharides, lipids, and nucleic acids that are commonly found in plants, plants, and microorganisms , Secondary metabolites are various compounds such as alkaloids, terpenes, flavonoids and antibiotics, which are mainly found in plants and microorganisms, and they are becoming useful as medicines, fragrances, raw materials for flavor and functional foods and the like. In the future, soil microorganisms occupy an important position in the biomaterial industry through separation of new strains and gene functions.

The secondary metabolites produced by the mushroom strain used as a fermenting microorganism have characteristics not found in other fungi. That is, the fermenting microorganisms Lentinula edodes , Phellinus linteus , Cordyceps militaris , Pleurotus ostreatus and Trametes versicolor have many metabolites. This means that the first metabolite is essential for the survival of the organism, but the significance of the second metabolite is still unclear. Antimicrobials, antimicrobials, enzymatic inhibitors, nucleic acid-related substances, amino acid-related substances, organic acids, phytohormones, and the like. Other physiologically active substances are peptides and protein-based physiologically active substances, which are still unknown, such as substances acting on insects, nematodes, germs, plant growth inhibitors, vitamin B1 breakdown substances and pharmacologically active substances .

In the fourth step of the present invention, the powder of the second step is mixed with a fermenting microorganism using an incubator, and a nitrogen source necessary for fermentation is added to produce a primary fermentation through a first biological reaction. In the fourth step, oyster shell powder, fermentation microorganism and nitrogen source necessary for fermentation are put on the incubator for the first fermentation, and the process is automated to stabilize the quality. The incubator referred to here is an aseptic system, and the temperature and humidity are automatically adjusted so that the oyster shell powder, the fermenting microorganism, and the nitrogen source necessary for fermentation are the incubator in which the proliferation and fermentation of the microorganism are simultaneously carried out.

At this time, in the fourth step according to the present invention, the fermenting microorganisms are mixed, and 30 to 70% by weight of nitrogen causing microorganisms are mixed and heated to a temperature of 25 to 40 ° C and a humidity of 50 to 80% Day fermentation to produce a fermented product. In order to produce the first fermentation product, the oyster shell powder, the fermentation microorganism and the nitrogen source were added to the incubator in an amount of 30 to 70% by weight to prepare an admixture. The mixture was then incubated at a temperature of 25 to 40 ° C and a humidity of 50 to 80% Under a condition that is maintained automatically, a primary fermentation product is produced so that a useful substance can be produced through a smooth primary biological reaction for 2 to 8 days.

In the fifth step of the present invention, the fermentation microorganism is sterilized and dried using the autoclave of the first fermentation product of the fourth step. The fifth step is a step of sterilizing and drying the primary fermentation product. In order to sterilize the primary fermentation product, it is desirable to introduce a dedicated automatic facility to improve quality stability and productivity.

At this time, in the fifth step according to the present invention, the primary fermentation product is sterilized and dried using a rapid autoclave at a temperature of 120 ° C. to 130 ° C. for 15 to 30 minutes during the biological reaction. The fermentation broth which has undergone the first bioreaction is reacted with a fermenting microorganism , Bacillus cereus, Bacillus natto, Aspergillus oryzae, Lactobacillus acidophilus, Lactobacillus bulgaricus , Lentinula edodes, Phellinus linteus, Cordyceps militaris, Pleurotus ostreatus and Trametes versicolor .

Meanwhile, in order to remove the fermenting microorganisms contained in the fermentation product in the fermentation step, the microorganism is rapidly sterilized and dried through an autoclave. If the fermentation microorganism is continuously fermented without sterilization, the quality of the product is lowered due to the characteristic odor of fermentation and at the same time the addition property is lowered.

In the case of rapid sterilization, it is generally used at 121 ° C for 10 to 30 minutes and at 132 ° C for 10 to 30 minutes. In the present invention, however, the fermentation microorganisms are effectively sterilized by drying at a treatment temperature of 120 to 130 ° C for 20 minutes .

In a sixth step of the present invention, the primary fermentation product of the fifth step is mixed with a fermenting microorganism using an incubator, and a nitrogen source necessary for fermentation is added to produce a secondary fermentation product through a secondary biological reaction. In the sixth step, the primary fermentation product, the fermentation microorganism, and the nitrogen source necessary for fermentation are put on the incubator to perform secondary fermentation, and the process is automated to stabilize the quality. The incubator referred to herein is an aseptic system, and refers to an incubator in which temperature and humidity are automatically adjusted so that primary fermentation products, fermenting microorganisms, and nitrogen sources necessary for fermentation simultaneously perform microbial growth and fermentation.

At this time, in the sixth step according to the present invention, the fermenting microorganisms are mixed and 30 to 70% by weight of nitrogen is caused to mix, and the mixture is stirred at a temperature of 25 to 40 ° C and a humidity of 50 to 80% Day fermentation to produce a fermented product. This is the same as the fourth step. That is, 30 to 70% by weight of the primary fermentation product, the fermentation microorganism and the nitrogen source were added to the incubator so as to produce the second fermentation product, Next, a secondary fermentation product is produced so that a useful substance can be produced while undergoing a smooth secondary biological reaction for 2 to 8 days at a temperature of 25 to 40 ° C and a humidity of 50 to 80% automatically maintained.

In the seventh step of the present invention, the fermentation microorganism is sterilized and dried using a rapid sterilization apparatus (Autoclave) in the sixth step. The seventh step is to perform the same operation as in step 5, that is, to sterilize and dry the secondary fermentation product. In particular, in order to sterilize the secondary fermentation product, a dedicated automatic facility is introduced It is good to do.

At this time, the seventh step according to the present invention sterilize and dry the fermenting microorganisms which are added during the biological reaction for 15 to 30 minutes at a temperature of 120 ° C to 130 ° C by using a autoclave. This is because the fermentation product subjected to the secondary bioreaction is reacted with a fermenting microorganism which has been put into a bioreactor at a temperature of 120 ° C to 130 ° C for 15-30 minutes in a rapid autoclave, Saccharomyces cerevisias, Bacillus natto, Aspergillus oryzae, Lactobacillus acidophilus, Lactobacillus bulgaricus , Lentinula edodes, Phellinus linteus, Cordyceps militaris, Pleurotus ostreatus and Trametes versicolor .

In the eighth step of the present invention, the secondary fermentation product of the seventh step is mixed with the fermentation microorganism using an incubator, and a nitrogen source necessary for fermentation is added to the fermentation product to produce a tertiary fermentation product through a tertiary bioreaction. The eighth step is a third fermentation step in which the secondary fermentation product, fermentation microorganism and nitrogen source necessary for fermentation are put on the incubator, thereby automating the process for quality stabilization. The incubator referred to here is an aseptic system, and the temperature and humidity are automatically adjusted to refer to an incubator in which secondary fermentation products, fermentation microorganisms, and nitrogen sources necessary for fermentation simultaneously perform microbial growth and fermentation.

The fermentation microorganisms are mixed and 30 to 70% by weight of nitrogen sources are mixed. The fermentation microorganisms are mixed at a temperature of 25 to 40 ° C and a humidity of 50 to 80% Day fermentation to produce a fermented product. This is the same as steps 4 and 6 above. That is, 30 to 70% by weight of a secondary fermentation product, a fermentation microorganism and a nitrogen source are added to an incubator to produce a third fermentation product. , And then a third fermentation product is produced so that a useful substance can be produced while undergoing a smooth third biological reaction for 2 to 8 days at a temperature of 25 to 40 ° C and a humidity of 50 to 80% automatically maintained.

In the ninth step of the present invention, the tertiary fermentation product of the eighth step is dried at a moisture content of 18% by using a drier. Step 9 is a step of drying the fermented product subjected to the primary, secondary, and tertiary bioreaction. It is preferable to introduce a dedicated automatic facility to improve the productivity in drying the fermented product. The fermented fermented product is dried at a moisture content of 18% by using a dryer to increase the concentration of the soluble component by reducing the volume of the liquid, thereby increasing the application range and widening the application range and improving the storage stability.

Step 10, which is the final step of the present invention, is a step of pulverizing the fermented product of step 9 with a powder of 60 to 80 mesh using a pulverizer to produce a fermented product. Step 10 is a step of pulverizing the fermentation product that has undergone the first, second and third bio-reactions. It is preferable to introduce a dedicated automated facility for improving the productivity in pulverizing fermentation products. This is accomplished by primary, secondary, and tertiary bioreactors, and the finished fermentation products are pulverized to produce 60 to 80 mesh powders. Powdered oyster shell fermentation products are prepared as powders to reduce the cost of storage, packaging and transportation of concentrated fermentation products, to widen the application range, to diversify applications, and to improve storage stability.

As described above, the omentophilic fermented product produced by the production method of the present invention has the effect of suppressing the chain of molecules of the effective substance contained in the oyster shell to a small unit , The active ingredient can be more effectively absorbed, and it can be used as a raw material for a variety of raw materials such as agricultural fertilizer raw materials including livestock bacteria, yeast, livestock feed additives using oyster shell fermentation products containing a large amount of calcium, etc. through multi- It is possible to mass-produce high-value-added materials in a large amount at low cost.

Hereinafter, the effect of the present invention will be described by examining functional components of the oyster shell fermentation product, that is, the number of viable cells and an enzyme experiment, a general component analysis, an inorganic analysis, an amino acid analysis and an aflatoxin analysis. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

[Experiments and results 1] Experiments on the number of live bacteria in oyster shell fermented products

Cell counting using a hemocytometer was performed as follows.

1. Inspection Principle

- Calculate the number of cells per unit volume by measuring the number of cells with a microscope using a hemocytometer with a space for a certain amount of cell suspension.

2. Appliances and equipment

1) Hemocytometer

2) CoverGlass

3) Pasteur pipette

4) Phase contrast microscope

3. Inspection method

1) Cover the cover glass with a clean, dry hemocytometer.

2) Take the washed cell solution with a Pasteur pipette and place in the chamber for about 3 minutes. At this time, all graduation areas are filled and not overflowed.

3) Place the chamber on a microscope and focus on X100 magnification at low magnification, and calculate the average value (X) by counting 4 large squares of 1 mm2 (WBC square). If the number of cells per square millimeter is 50-100, it is appropriate to dilute the cell solution more than 100 cells.

WBC No. (/ ㎣) = X (number of cells / mm 2) × 0

WBC No. (/ mL) = X (number of cells / mm 2) × 10 × 000

(For example, X is 100 → 1000 / ㎣ = 1 × 10 6 / mL)

* Or calculate the number of cells at × 400 times in RBC calculation square.

Through the above calculation method, oyster shell powder was analyzed by cell counting method using hemocytometer, and the following results were obtained.

[Experiments and results 2] Enzyme experiments on oyster shell fermented products

According to the Korea Food and Drug Administration, the following results were obtained.

[Experiments and results 3] Analysis of general components of oyster shell fermentation products

General component analysis was performed according to the method of Association of Official Analytical Chemists (AOAC). Moisture was analyzed by 105 ℃ atmospheric heating drying method, crude protein was analyzed by Kjeldahl method, and questioned by 550 ℃.

[Experiments and results 4] Mineral analysis of oyster shell fermentation products

Calcium and phosphorus were added to 10 g of the sample and allowed to stand at room temperature for 12 hours or more. The mixture was heated at 100 ° C. for 24 hours or more until the solution became a clear yellow solution. Nitric acid was added to the reaction solution and the reaction was continued until the acid completely evaporated to remove the organic matter. Thereafter, 20 mL of 0.2 N nitric acid was added to the sample solution, and the solution was eluted for 24 hours. The solution was filtered with a 0.45 μm membrane filter, and analyzed by ICP (Inductively Coupled Plasma, Varian 720-ES, Varian Medical Systems, Inc., USA).

[Experiments and results 5] Amino acid analysis of oyster shell fermentation products

Amino acid components were sampled at 1 g, placed in a test tube, 15 mL of 6N HCl solution was added, and the mixture was subjected to acid hydrolysis in a dry oven at 110 ° C for 24 hours. The hydrolyzed lysate was filtered with a glass filter and the filtrate obtained for complete evaporation of hydrochloric acid and water was concentrated under reduced pressure at 55 ° C. Concentrated samples were filtered with a 0.45 μm membrane filter using sodium citrate buffer (pH 2.2), and the constituent amino acid content was determined using an amino acid automatic analyzer (Biochrom 20, Pharmacia Biotech., Ltd., U.K.).

[Experiments and results 6] Aflatoxin analysis of oyster shell fermented products

Aflatoxin, a fungal toxin, was analyzed using an ELISA assay.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. It is therefore intended that such variations and modifications fall within the scope of the appended claims.

Claims (5)

A first step of washing with oyster shell as a raw material and then drying;
A second step of crushing the dried material of the first step to produce powder, and sterilizing the bacterium using a rapid sterilization apparatus (Autoclave);
A third step of culturing the fermentation microorganism using the bioreactor in the second step;
A fourth step of mixing the powder of the second step with a fermenting microorganism using an incubator, adding a nitrogen source necessary for fermentation to produce a first fermentation product through a first biological reaction;
A fifth step of sterilizing and drying the fermenting microorganism using a rapid sterilization apparatus (Autoclave) in the fourth step of fermentation;
A sixth step of mixing the primary fermentation product with the fermentation microorganism using an incubator and adding a nitrogen source necessary for fermentation to produce a secondary fermentation product through a secondary biological reaction;
A seventh step of sterilizing and drying the fermentation microorganism using a rapid sterilization apparatus (Autoclave) in the sixth fermentation step;
An eighth step of mixing the secondary fermentation product of step 7 with a fermenting microorganism using an incubator, adding a nitrogen source necessary for fermentation to produce a tertiary fermentation product through a tertiary bioreaction;
A ninth step of drying the third fermentation product of step 8 at a moisture content of 18% using a drier; And
The method of claim 10, wherein the fermented product is pulverized using a pulverizer to a size of 60 to 80 mesh to produce a fermented product. The method according to claim 1,
Wherein the second step is to produce oyster shell dried material as a powder of 0.5 to 1 cm with a crusher and sterilize the microorganism at a temperature of 100 to 130 ° C for 15 to 25 minutes using a rapid sterilization apparatus. ≪ / RTI > The method according to claim 1,
Wherein the third step is a step of isolating the fermenting microorganisms Saccharomyces cerevisis, Bacillus natto, Aspergillus oryzae, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lentinula edodes, Phellinus linteus, Cordyceps militaris, Pleurotus ostreatus, Trametes versicolor is cultured to a population of 10 ^{+ 1} to 10 ^{+ 7} per ml of the culture medium, and the mixture is prepared singly or as a mixture, thereby producing a fermented oyster product using the microorganism. The method according to claim 1,
The fermentation microorganisms are mixed with 30 to 70% by weight of nitrogen sources and incubated at a temperature of 25 to 40 ° C and a humidity of 50 to 80% And fermenting the fermented product for 8 days to produce each fermented product. The method according to claim 1,
Wherein the fermentation product is sterilized and dried using a rapid autoclave at a temperature of 120 ° C. to 130 ° C. for 15 to 30 minutes during the biological reaction. A method for producing an oyster shell fermentation product.

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