KR20190081204A - Method for producing organic fertilizer using blood of animal by-product - Google Patents

Abstract

The present invention relates to a method for manufacturing organic fertilizer using animal blood which is a byproduct of slaughter. According to the present invention, it is possible to remarkably reduce a large amount of blood waste generated during a slaughter process, and expect an effect of reducing costs for waste water treatment and a great economic effect by creating a compost sales profit. Also, a useful microorganism group (microbial agents) to be added to a byproduct includes a large amount of microorganism which performs a function of promoting decomposition of various organic materials and is capable of improving the quality of soil, and particularly, citric acid-cinnamon extract is injected after fermentation to remarkably reduce bad smell generated in a fermentation step, thereby causing no smell unlike excreta compost. Accordingly, a packaging unit is manufactured in various forms, and thus the organic fertilizer of the present invention can be used for various purposes such as outdoors, facility plantation, flower, home and the like.

Description

Technical Field [0001] The present invention relates to a method for producing an organic fertilizer using an animal blood, which is a by-product of slaughter,

The present invention relates to a method for producing an organic fertilizer using an animal blood, which is a by-product of slaughter.

Recently, contaminated rivers have been introduced into waters and deteriorated in water quality. Therefore, they are expected to show new trends in Korean environmental policy and environmental technology by showing red tides, green tides, and fishery populations in the coastal waters. In the livestock industry, the pig industry is expected to continue to grow along with the increase of the national income, and it is expected that the consumption of pork will continue to increase. Therefore, in the production sector of pork pork, international competitiveness should be improved by improving the productivity of livestock technology. In addition, environmental pollution caused by the excrement of livestock in the production process of pork and pork and waste Technologies that can reduce environmental pollution must also be developed at the same time.

The environmental pollution that occurred during the production process of pork and pork was developed in 1991 and many technologies such as composting of sewage and manure have been developed since the promulgation of the Act on Disposal of Sewage Manure and Livestock Wastewater, The countermeasures against contamination caused by livestock blood are relatively insignificant. Blood generated during the slaughter process is a source of pollution, but if it is used for other purposes, the environment can be solved through various methods of use and it can be used as a business with high economic profit.

The government also declares the integration of the environment and economy in the basic principles for the construction of the environmental community, and emphasizes the principle of precaution and polluter burden. Most of the livestock farmers are facing high costs due to pollution prevention and disposal costs due to the emphasis on this principle. Even if the livestock farmers solve the problem of wastewater pollution, they can not slaughter at the slaughterhouse. Slaughterhouse The seriousness of the environmental problem has been discussed for a long time, but no specific solution has been found. The best policy to solve these environmental and agricultural problems at the same time is to prevent pollution and not to dispose pollutants but to industrialize resources.

Generally, in order to treat 1 ton of livestock blood, more than 5 tons of groundwater or water sources are required. Most of them are discharged to the own wastewater treatment facility, and the cost of the treatment is high, or when the wastewater is treated by transporting it to the wastewater treatment plant Considering the fact that the cost of processing wastewater from 400 ~ 5 million won per month for small slaughterhouses that process 300 ~ 400 eggs a day, it is possible to reduce pollution of the environment by producing other useful materials by using livestock blood In addition, it is very desirable to expand the production base of domestic livestock in the future with the creation of demand for new goods, since it can be small but it can help.

Many organic composts are being developed and produced domestically and internationally. In particular, high-function hemp compost is produced and sold through various methods. However, since most of dried blobs are used for pretreatment costs, they are sold at about 35,000 ~ 40,000 won per 20kg. It has the disadvantage that the price is about twice the price. Blood compost is produced outside the country and it is imported and sold, but it is also high compared to domestic production of blood compost. Therefore, some companies are attempting to compost blood directly, but most of them are failing due to odor generated in composting process because blood itself contains high concentration protein.

The present invention is a revolutionary method for direct composting of animal blood which can be economical in terms of cost compared with existing blood compost. The odor generated during composting can be solved by using citric acid-cinnamon extract. Therefore, the present invention not only solves the environmental pollution problem of the slaughter blood, but also can significantly reduce the waste water treatment cost by utilizing the whole slaughter blood, and it is expected that additional profit can be generated by producing high-function compost.

Korean Patent Laid-Open Publication No. 2002-0022416 discloses a method for producing organic fertilizer based on blood, and Korean Patent Laid-Open Publication No. 2015-0143903 discloses a method for producing liquid fertilizer using slaughter blood However, as in the present invention, there is no known method for producing an organic fertilizer using animal blood, which is a slaughter byproduct.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a method of disinfecting animal blood, which is generated during slaughtering, together with washing water. In addition to causing costs and environmental problems, additional costs are incurred due to the expansion of waste treatment facilities. In addition, when food and feed are used, only pure blood must be collected separately. Therefore, I realized that I needed to spend money.

Therefore, in the present invention, the blood generated in the slaughtering process is collected in a state containing washing water without any other pretreatment, and the microorganism preparation is blended with sawdust, rice bran, rice husk, In order to remove the citric acid-cinnamon extract, it is firstly composted, and after the composting process, the functional organic fertilizer with high quality and amino acid-rich as composted compost is developed. The present inventors have completed the present invention by confirming that it is possible to expect the effect of the income gain when the income is guaranteed.

In order to achieve the object of the present invention,

(a) blending animal blood, sawdust, rice husks, rice bran, and coffee by-products at a weight ratio of 3.5 to 4.5: 2.5 to 3.5: 1 to 2: 1.5 to 2.5: 0.5 to 1.5 to prepare a compounding material;

(b) adding 1 to 5% by weight of a microorganism preparation based on the total weight to the compounding material of step (a) and mixing the mixture;

(c) adjusting the moisture content of the compounding material mixed with the microbial agent of step (b) to 60 to 70% for initial fermentation for 3 to 4 weeks;

(d) subjecting the initial fermentation product of step (c) to primary compaction for 8 to 9 weeks; And

(e) post-boiling the first composted water of step (d) for 11 to 12 weeks. The method for producing an organic fertilizer using animal blood as a by-product of slaughter is provided.

The present invention also provides an organic fertilizer using animal blood, which is a slaughter by-product produced by the above method.

The present invention is expected to greatly reduce the amount of blood waste water generated during the slaughter process and to reduce the cost of wastewater treatment. In addition, the useful microorganism group (microorganism preparation) to be added to the by-product contains many microorganisms capable of improving and improving the quality of the soil as well as the function of accelerating the decomposition of various organic matters. In particular, citric acid- It is possible to dramatically reduce the odor generated in the fermentation stage, and it is possible to manufacture the packaging unit in various forms because there is no odor unlike the manure compost, etc., so that it can be utilized for various purposes such as the open field, the facility cultivation field, .

1 shows a process of manufacturing a compost according to the present invention.
FIG. 2 shows changes in microorganisms (A is a bacterium, B is a mold), temperature (C) and pH (D) during composting progress of the sample D.
Fig. 3 shows a chromogram for the amino acid analysis of sample D. Fig.

In order to achieve the object of the present invention,

(a) blending animal blood, sawdust, rice husks, rice bran, and coffee by-products at a weight ratio of 3.5 to 4.5: 2.5 to 3.5: 1 to 2: 1.5 to 2.5: 0.5 to 1.5 to prepare a compounding material;

(b) adding 1 to 5% by weight of a microorganism preparation based on the total weight to the compounding material of step (a) and mixing the mixture;

(c) adjusting the moisture content of the compounding material mixed with the microbial agent of step (b) to 60 to 70% for initial fermentation for 3 to 4 weeks;

(d) subjecting the initial fermentation product of step (c) to primary compaction for 8 to 9 weeks; And

and (e) post-humidifying the first compost of the step (d) for 11 to 12 weeks. The method for producing an organic fertilizer using animal blood, which is a by-product of slaughter,

(a) blending animal blood, sawdust, rice husks, rice bran, and coffee by-products at a weight ratio of 4: 3: 1.5: 2: 1 to prepare a compounding material;

(b) adding 1 to 5% by weight of a microorganism preparation based on the total weight to the compounding material of step (a) and mixing the mixture;

(c) adjusting the moisture content of the compounding material mixed with the microbial agent of step (b) to 60 to 70% for initial fermentation for 3 weeks;

(d) first fermenting the initial fermentation of step (c) for 8 weeks; And

(e) disinfection of the first compost of the step (d) for 12 weeks. The method of manufacturing an organic fertilizer using animal blood as a by-product of slaughter, however, is not limited thereto.

In the method according to one embodiment of the present invention, citric acid-cinnamon extract obtained by extracting cinnamon with a citric acid solution in step (c) is sprayed at 1 to 2 liters per 100 kg of the compounding material once every 1.8 to 2.2 weeks after the start of fermentation, Preferably, the citric acid-cinnamon extract obtained by extracting cinnamon with a citric acid solution in step (c) is sprayed at 1 to 2 L per 100 kg of the compounding material once every two weeks after the start of fermentation, and the initial fermentation can be performed But is not limited thereto.

In the method according to one embodiment of the present invention, the citric acid-cinnamon extract is prepared by adding 0.015 to 0.025% (w / v) cinnamon slices to 0.8-1.2 M citric acid solution and heating at 70-80 ° C for 48-72 hours And preferably 0.02% (w / v) cinnamon slices are added to a 1 M citric acid solution, and the mixture is heat-extracted at 70 to 80 ° C for 48 hours. However, the present invention is not limited thereto.

The injection of the citric acid-cinnamon extract obtained by extracting the cinnamon with the citric acid solution of the present invention is sprayed to the initial fermentation stage during the manufacturing process of the organic fertilizer using the animal blood of the present invention to neutralize the ammonia and amine-based gases generated during the fermentation process The odor can be reduced.

In the method according to an embodiment of the present invention, the compounding material of step (a) may additionally include humus, but is not limited thereto. The compounding material to which the humus is added is selected from the group consisting of animal blood, sawdust, rice husk, rice bran, coffee by-products and humus in a weight ratio of 3.5 to 4.5: 2.5 to 3.5: 1 to 2: 1.5 to 2.5: 0.5 to 1.5: 0.2 to 0.3, May be formulated in a weight ratio of 4: 3: 1.5: 2: 1: 0.25, but is not limited thereto.

In the method according to one embodiment of the present invention, the microorganism preparation may be one in which the same amount of sawdust is added to the dried product of 17 kinds of microbial culture solutions added in the same amount of 10 ⁹ to 10 ¹² cfu / ml, Do not. Preferably, the 17 kinds of strains of bacteria is Bacillus Lee Kenny Po Ms (Bacillus licheniformis) and 11 species, the fungus Aspergillus or this (Aspegilus niger) and other 2 species, the yeast is Saccharomyces as MY process three Levy jiae (Saccharomyces cerevisae ) consists of one kind other, more preferably Bacillus Lee Kenny Po Ms (Bacillus licheniformis), Bacillus standing subtilis (Bacillus subtilus), Bacillus amyl Lowry Quebec Pacific Enschede (Bacillus amyloliquefaciens), Cellulofine Pseudomonas UDA (Cellulomonas uda) , Cellulomonas cellulans cellulans , Clostridium acetobutylicum , Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus bulgaricus , Micrococcus luteus, leuteus), Pseudomonas fluoro lesson's (Pseudomonas fluorescence), by jelriah cibaria (Weisselia cibaria), moving teuken Escherichia Oriental less (Issatchenkia orientalis), Celebi as Saccharomyces My jiae access (Saccharomyces cerevisae , Arspegilus niger , Rhizopus oryzae , and Rhizomucor pusillus , but are not limited thereto.

The present invention also provides an organic fertilizer using animal blood, which is a slaughter by-product produced by the above method. The organic fertilizer includes animal blood as a slaughter by-product produced by the above method as a main component, and can be used according to a method commonly used in the art.

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example  1. Preparation of useful microbial agents used in the present invention

In order to prepare the useful microorganism preparation, the compost-related strains were pre-cultured from the strains isolated from the present invention and the Korean Microorganism Conservation Center (KCCM) and stored at -4 ° C to be used as a large-scale culture. Bacteria Bacillus Lee Kenny Po Ms (Bacillus licheniformis) and 11 species, the fungus Aspergillus or this (Aspegilus niger) and other 2 species, the yeast is a Saccharomyces three Levy MY process jiae (Saccharomyces cerevisae ) and 17 species (Table 1). Species were selected considering various substrate degradation characteristics, efficacy, culture conditions and odor removal characteristics.

The culture of the microorganism was carried out by using the culture conditions (temperature, pH, stirring) of the microorganism and the microorganism medium as shown in Table 1. 5 ~ 10 ml of pre - cultured seedlings were inoculated into 10 ~ 15 L of medium and cultured for 24 ~ 72 hours according to temperature and medium conditions. To measure the number of cultured microorganisms, the culture was diluted to 10 ^-1 to 10 ^-9 by serial dilution, and 0.1 ml of each dilution was inoculated into each of the three plate media containing each medium (tri-plate method) The colonies (cfu / ml) formed after culturing for 72 hours were counted. As a result, 10 ⁹ to 10 ¹² cfu / ml populations were analyzed depending on the type of microorganism.

Drying sawdust was used as a substrate for microbial immobilization to prepare microbial agents. The mass cultured microorganism was mixed with the same amount of substrate (100 L of culture medium + 100 kg of substrate), and then naturally dried at room temperature (20 ~ 25 ° C) for 4 ~ 7 days. 1 g of the prepared microorganism preparation was added to 20 ml of sterilized water and mixed thoroughly. 1 ml of the diluted solution was added to a test tube containing 9 ml of sterilized water, followed by serial dilution (to 10 ^-8 ) The colonies were counted by inoculation with 0.1 ml of tri-plate method (3 plate culture medium) in nutrient agar medium and PDA (potato dextrose agar) medium and culturing at 30 ° C and 37 ° C for 24-48 hours. As a result, if the bacteria in 30 ℃ the 3.2 x 10 ¹¹ to cfu / g, 37 ℃ was more than 7.6 x ¹⁰ 10 cfu / g, when the fungus was cultured at 26 ℃ 48 ~ 72 hours, the resulting 4.6 x 10 ⁸ cfu / g. < / RTI > This indicated that more microorganisms were immobilized than expected.

List of microorganisms (bacteria, yeast and fungi) used in the manufacture of microorganism preparations Strain name Culture conditions Culture temperature (캜) Culture medium Bacillus licheniformis Aerobic bacteria 45 BHI Bacillus subtilus Flowable anaerobic bacteria 30 Nutrient Bacillus amyloliquefaciens Flowable anaerobic bacteria 37 Medium 211 Cellulomonas uda Flowable anaerobic bacteria 30 PTYG Cellulomonas cellulans Flowable anaerobic bacteria 30 Nutrient Clostridium acetobutylicum Flowable anaerobic bacteria 30 RCM Lactobacillus acidophilus Flowable anaerobic bacteria 37 MRS Lactobacillus casei Flowable anaerobic bacteria 37 MRS Lactobacillus bulgaricus Flowable anaerobic bacteria 37 MRS Micrococcus leuteus Flowable anaerobic bacteria 30 Nutrient Pseudomonas  fluorescence, Flowable anaerobic bacteria 30 Nutrient Weisselia cibaria Flowable anaerobic bacteria 39 MRS Issatchenkia orientalis Aerobic bacteria 25 YM Saccharomyces cerevisae Flowable anaerobic bacteria 25 YM Aspegilus  niger Flowable anaerobic bacteria 30 PDA or YM Rhizopus oryzae Flowable anaerobic bacteria 30 PDA or YM Rhizomucor pusillus Flowable anaerobic bacteria 30 PDA or YM

The prepared microorganism preparation was stored at room temperature and 1 g of each sample was collected at intervals of 15 days to measure the number of microorganisms. The survival rate of the microorganisms in the preparation was analyzed for about 105 days. The results are shown in Table 2. In the case of bacteria, 10 ⁸ to 10 ⁹ cfu / g populations were maintained for 1 month, and 10 ⁵ to 10 ⁶ cfu / g populations survived after about 3 months. If mold even after one month of about 10 ⁷ was judged to cfu / g, it exhibited a relatively gentle mortality rate by maintaining the about three months at the point in time at 10 ⁵ cfu / g populations as better survival.

Microbial survival rate of microbial agent (unit: cfu / g) date Bacteria Fungi 30 ℃ 37 ℃ 26 ~ 30 ℃ 0 3.2 x 10 ¹¹ 7.6 x 10 ¹⁰ 4.6 x 10 ⁸ 15 3.9 x 10 ¹⁰ 1.4 x 10 ¹⁰ 3.2 x 10 ⁸ 30 5.2 x 10 ⁹ 8.4 x 10 ⁸ 8.8 x 10 ⁷ 45 1.3 x 10 ⁸ 6.7 x 10 ⁷ 3.6 x 10 ⁷ 60 2.2 x 10 ⁷ 1.2 x 10 ⁷ 2.5 x 10 ⁷ 75 6.4 x 10 ⁶ 4.8 x 10 ⁶ 3.0 x 10 ⁶ 105 1.8 x 10 ⁶ 8.0 x 10 ⁵ 2.0 x 10 ⁵

Example  2. Preparation of citric acid-cinnamon extract and deodorization test

Citric acid (C ₆ H ₇ O ₈ H ₂ O) is an acidic substance that has been used as an additive for livestock disinfectants, detergents and soft drinks, and is known to be harmless to humans. Cinnamomum cassia ( Cinamomum cassia ) is a plant belonging to the dicotyledonous plants of the dicotyledonous plant, which is called cinnamon. Cinnamon aldehyde is a typical component of cinnamon and has been used as a main additive for Chinese medicine because of its various effects in oriental medicine. Cinnamon is also used as an insect repellent (mosquito, flies, etc.).

In the present invention, a 1 M solution of citric acid was prepared for use as a deodorant and insect repellent in combination with 200 g of dry cinnamon, and the mixture was subjected to hot water extraction at 70 to 80 ° C for 48 to 72 hours to prepare citric acid-cinnamon extract Respectively. In order to confirm the deodorizing effect of the material, a deodorizing effect was confirmed by asking KCL (Korea Environment & Construction Research Institute) for a dilution of 100 times. Therefore, this material is used in the initial fermentation step during the compost manufacturing process of the present invention to neutralize the ammonia and amine-based gases generated during the fermentation process to reduce odor. In order to confirm the deodorizing effect of compost, 100g of the initial fermented compost (2 nd week) in active fermentation is taken, 10ml of citric acid-cinnamon extract is sprayed in 10ml, 1g of 100g of compost is taken and 20ml of distilled water is added As a result of pH measurement, it was found that the compost without added extract retained its alkalinity at pH 8.6, but the compost from which the extract was sprayed showed a neutral pH of 7.5, which was excellent in neutralizing the alkali and had almost no odor .

Trimethylamine and ammonia deodorization test (KCL) Test Items unit Test result Exam conditions Blank concentration
(umol / mol) Sample concentration
(umol / mol) Concentration reduction rate
(%) Trimethyl
Amine,
ammonia 0 minutes % 50 50 0.0 24.9 DEG C 30 minutes 49 One 98.0 60 minutes 49 <0.2 99.6 90 minutes 49 <0.2 99.6 120 minutes 49 <0.2 99.6

※ detection limit 0.2 μmol / mol

Example  3. By-product of slaughter Animal blood  Manufacture of compost using

C: Carbon ratio of material N: Nitrogen content of the material R: Carbonitride to be calibrated) The ratio of the carbon content of the compost is 30% of the corrected carbon content M = C / R-N.

For example, when sawdust is used as a carbon source, M = 46 / 30-0.05 = 1,48% when adjusted to 46% of carbon (C), 0.05% of nitrogen (N) Therefore, since the amount of nitrogen to be added to 1,000 kg is 14.8 kg, it was adjusted by adding rice bran and blood containing a large amount of nitrogen component. When the rice husk was used as the carbon source, M = 36.3 / 30-0.48 = 0.73% when adjusted to 36.3% of the carbon (C) component, 0.48% of the nitrogen component and 30% of the corrected carbonitride ratio, The amount is 7.3 kg. Using the above calculation method, composting was carried out at a total of five blending ratios (Table 4).

Compost composition by sample (Unit: kg) Sample byproduct Sample A ( % ) Sample B ( % ) Sample C ( % ) Sample D ( % ) Sample E ( % ) Animal blood 160 (27) 80 (30) 60 (25) 80 (32) 80 (35) sawdust 200 (34) 120 (40) - 60 (25) 60 (30) chaff 100 (17) - 80 (32) 30 (12) 30 (15) Rice bran 60 (10) 40 (15) 40 (18) 40 (16) 40 (18) Coffee byproduct 20 (3) 10 (5) 10 (5) 20 (10) - Humus 10 (1.5) 5 (3) 10 (5) 5 (2.5) 5 (3) Microbial agent 20 (3) 10 (5) 10 (5) 10 (5) - Humidity(%) 60 to 70 60 to 70 60 to 70 60 to 70 60 to 70 Total amount 600 kg 300 kg 250 kg 250 kg 220 kg

Sample A was prepared by adding the sawdust and rice hulls as a carbon source at a ratio of about 2: 1 and adjusting the blood weight ratio to 27%. The initial fermentation stage (about 3 weeks), the primary composting stage (about 8 weeks) After 6 months, the composting was carried out after the straw. Sample B was prepared by adding sawdust as a carbon source, adjusting the blood weight ratio to 30% and composting in the same manner. Sample C used chaff as a main carbon source and adjusted the blood weight ratio to 25% for composting. Samples D and E were added with sawdust and rice hulls as a carbon source at a ratio of 2: 1, and the weight ratio of blood was adjusted to 32% and 35%, respectively. Sample E was composted without adding microbial agents and coffee byproducts And to compare the effects of microbial agents and coffee byproducts. These two composts were composted indoors.

Humidity is adjusted to 60 to 70% in all combinations. Humus added in addition to sawdust, rice bran, blood, coffee by-products and microbial agents further promotes compost composting by addition of various microorganisms in addition to the microorganisms contained in the added microorganism preparation .

The composting process to be accomplished in the present invention was carried out with a slaughterhouse-linked system (Fig. 1). A step of mixing a blood, a microorganism preparation, a compost source (sawdust, rice husk, rice bran, coffee by-products, humus, etc.) of an animal blood collecting tank including washing water generated in a slaughterhouse at an appropriate ratio, The citric acid-cinnamon extract was added to the mixture at a rate of 1 per 100 kg of the compounding material during the flipping process. The mixture was adjusted to a moisture content of 60 to 70% by using blood and washing water and the mixed ingredients (initial fermentation step) &Lt; / RTI &gt; The composting step 1 was carried out with at least 8 weeks and 2 weeks once with flipping (stirring). The post - maturation stage was continued for about 12 weeks. During this period, flipping was performed once a month. The time of composting was judged by measuring the temperature and pH of compost and compost. Microbial dynamics, temperature, and pH were measured at the interval of 2 weeks until the composting stage and once a month at the composting stage. For reference, the compost of the present invention means a compounding material.

Example  4. Slaughter by-product Animal blood  Of compost produced using Degree of fermentation  analysis

The composting progress of each compost can be judged by comprehending the microbial dynamics during compost composting period. Microbial population analysis was performed by collecting 10 g of each of the compost surface and core, mixing 1 g of the mixture, adding 20 ml of sterilized water, and mixing thoroughly. One ml of each sample was serially diluted (10 ³ to 10 ⁵ ) in a test tube and 0.1 ml of each dilution was inoculated into three nutrient agar plates (tri-plate method) at 30 ° C and 37 ° C After culturing in an incubator for 24 to 48 hours, colonies were counted and expressed as a mean value. Samples were also prepared by the same method, and 0.1 ml of each sample was diluted in a PDA (potato dextrose agar plate), and the colonies were cultured for 48 to 72 hours at 25 to 30 ° C in an incubator.

In the initial mixing stage of each sample, the number of bacteria was in the range of 2.0 × ^{10 6} to 6.4 × ^{10 8} cfu / g and 3.4 × ^{10 6} to 9.2 × ^{10 8} cfu / g at 30 ° C and 37 ° C, respectively. From 1 week after the active composting Showed the highest individual density (10 ⁸ to 10 ¹⁰ cfu / g) within three weeks. After about 6 weeks, it tended to stabilize at an individual density of 10 ⁵ to 10 ⁶ cfu / g. In the case of fungi, the highest individual density (10 ⁶ to 10 ⁸ cfu / g) was observed between 2 and 4 weeks, and relatively stable individual densities after 5 weeks. Fig. 2 shows the change in the number of microorganisms in the sample D. Fig.

In addition, samples A, B, C and E showed a similar pattern to that of sample D in bacterial population changes, and the fungal population density was smaller overall than bacteria. In particular, samples D and E contained less than one-third of composts than sample A, and the highest density of microbial populations did not exceed 10 ⁸ cfu / g. In addition, sample E was composted without addition of the microbial agent, but there was no significant difference in the number of microorganisms compared to the sample D. This suggests that the microorganisms present in the compost source and humus have a great effect on the composting, and the microorganisms in the microorganism preparation can not be detected.

Changes in temperature during the composting process seem to be highly correlated with microbial population density. In all the samples, the maximum value was shown at 50 ~ 60 ℃ within 1 ~ 3 weeks after mixing the compost. From 6 weeks, it decreased below 50 ℃, and after 8 weeks, it remained at room temperature (Figure 2C). The pH of the compost was maintained at alkaline pH (above 8.0) for 2 ~ 7 weeks and then maintained at neutral pH (7.0 ~ 7.5) (Fig. 2D). These results indicate that the fermentation of the compost is most active between 1 week and 3 weeks, and the fermentation takes place gradually until 7 weeks. After 8 weeks, it persists for 8 ~ 12 weeks. Therefore, composting completion time is expected to be at least 16 weeks and up to 24 weeks. It is reported that when the compost fermentation temperature is maintained at 50 ℃ ~ 60 ℃ for 2 weeks, the microorganisms, parasites, eggs and weeds in the compost are killed. Therefore, it is judged that all of these conditions are satisfied in the present invention.

The type of compost is divided into several according to raw materials. In Korea, composting of livestock is dominant. Recently, earthworms and composts are classified as by-product compost when they are used as raw materials, which is also referred to as organic fertilizer. Normally, composting standards are unified based on composting of livestock including blood.

In the present invention, analysis of compost quality was commissioned and analyzed by the Agricultural Research Institute. The method of analysis was based on quality inspection of fertilizer according to Notification No. 2017-1 of RDA. Sixteen items including moisture were analyzed. The contents of phosphoric acid and carly were analyzed in addition to nitrogen, which is the three major elements of fertilizer, and the content of goto was further analyzed. The results are shown in Table 4. In all items, Korea met the criteria. In the case of sample D, it was found to meet the Dutch "green compost" criteria, which set the ideal standard as compost. In addition, when the contents of the three major components of compost for domestic animals are more than 1%, the organic fertilizer is defined as the organic fertilizer.

Analysis of compost specifications and other ingredients for each sample Analysis item Specification standard Sample A Sample B Sample C Sample D Sample E moisture(%) In the spot 55 or less 51.3 54.8 52.9 42.7 49.2 Organic matter (%) 30 or more 31.1 32.2 31.4 43.7 33.5 nitrogen(%) - 1.77 1.63 1.95 2.07 1.95 Organics / Nitrogen 45 or less 17.6 19.7 16.1 21.1 17.2 Arsenic (mg / kg) In building 45 or less Non-detection Non-detection Non-detection 0.2 0.1 Cadmium (mg / kg) 5 or less Non-detection 0.2 Non-detection Non-detection 0.4 Mercury (mg / kg) 2 or less 0.02 0.01 0.01 0.01 0.01 Lead (mg / kg) 130 or less 13.8 13.5 10.8 11.9 11.2 Chromium (mg / kg) 200 or less 0.9 0.6 1.5 1.9 1.1 Copper (mg / kg) Less than 360 6.1 5.4 7.4 Non-detection 5.8 Nickel (mg / kg) 45 or less 0.5 0.7 1.1 0.8 0.6 Zinc (mg / kg) 900 or less 30.7 30.6 46.7 41.5 39.5 salt(%) 2.0 or less 0.1 0.1 0.1 0.3 0.2 Mechanical compaction Completed (Sorbita) Compartmentalized Compartmentalized Compartmentalized Compartmentalized Compartmentalized Escherichia coli (0157: H7) Non-detection Non-detection Non-detection Non-detection Non-detection Non-detection Salmonella Non-detection Non-detection Non-detection Non-detection Non-detection Non-detection Hydrochloric acid insoluble seawater 25 or less 5.8 1.1 6.7 9.9 14.9 Phosphoric acid (%) - 1.48 2.37 2.82 2.85 2.90 Carly (%) - 0.77 0.94 1.16 2.11 1.77 Goto (%) - 0.42 0.59 0.88 1.15 0.94

For the analysis of amino acids in the samples, 1 g of each sample was sampled and 20 ml of sterilized water was mixed thoroughly and centrifuged (10,000 rpm). 1 ml of the sample was taken to longobio (logobio.com) Respectively. The instrument used was a free amino acid analysis by HPLC 1100 series (Hewlett Packard, USA). For the analysis, 40 ~ 100ul of each sample is dried and dissolved in 20: 1 mixture of methanol: water: triethylaminine: phenylisothiocyanate = 7: 1: 1: 1 and allowed to react at room temperature for 30 minutes. The reaction solution was dried and dissolved in 400 μl of HPLC A solvent, filtered through a 0.45 μm filter, and analyzed by injecting 10 μl into HPLC. The results of the analysis are shown in Table 6 and FIG. Only 7 ~ 8 kinds of amino acids were detected in all specimens. In particular, proline showed a specific pattern occupying more than 70% of the whole. Total amino acid content was relatively high, 7 ~ 70 mg / 100 ml. However, only 7 ~ 8 kinds of amino acids were detected and the imbalance was severe. It is considered that this is the result of measuring only free amino acid except structural amino acid. It is considered that amino acid containing more various amino acids and more concentration will be included in the case of including the whole structural amino acid of solid compost.

Amino acid content of each sample Sample amino acid Sample A
(mg / 100 ml ) Sample B
(mg / 100 ml ) Sample C
(mg / 100 ml ) Sample D
(mg / 100 ml ) Sample E
(mg / 100 ml ) Asp 0.00 0.00 0.00 0.04 0.14 Glu 0.00 0.00 0.00 0.00 0.00 Asn 1.86 1.78 1.74 1.76 1.70 Ser 0.00 0.00 0.00 0.00 0.00 Gln 0.04 0.14 0.12 0.19 0.38 Gly 0.15 0.11 0.10 0.08 0.17 His 0.00 0.00 0.00 0.00 0.00 Arg 0.00 0.00 0.00 0.00 0.00 Thr 0.00 0.00 0.00 0.00 0.00 Ala 0.00 0.00 0.00 0.00 0.00 Pro 9.64 5.75 5.10 32.74 68.64 Tyr 0.17 0.30 0.35 0.67 0.92 Val 0.00 0.00 0.00 0.00 0.00 Met 0.02 0.04 0.30 0.04 0.07 Cys 0.00 0.00 0.00 0.00 0.00 Ilu 0.04 0.16 0.90 0.19 0.32 Leu 0.00 0.00 0.00 0.00 0.00 Phe 0.00 0.00 0.00 0.00 0.00 Trp 0.00 0.00 0.00 0.00 0.00 Lys 0.00 0.00 0.00 0.00 0.00 Total 11.9 8.3 7.5 35.7 72.3

Claims (6)

(a) blending animal blood, sawdust, rice husks, rice bran, and coffee by-products at a weight ratio of 3.5 to 4.5: 2.5 to 3.5: 1 to 2: 1.5 to 2.5: 0.5 to 1.5 to prepare a compounding material;
(b) adding 1 to 5% by weight of a microorganism preparation based on the total weight to the compounding material of step (a) and mixing the mixture;
(c) adjusting the moisture content of the compounding material mixed with the microbial agent of step (b) to 60 to 70% for initial fermentation for 3 to 4 weeks;
(d) subjecting the initial fermentation product of step (c) to primary compaction for 8 to 9 weeks; And
(e) further refluxing the first composted water of step (d) for 11 to 12 weeks. The method according to claim 1, wherein in step (c), citric acid-cinnamon extract obtained by extracting cinnamon with citric acid solution is injected at 1 to 2 liters per 100 kg of the compounding material once every 1.8 to 2.2 weeks after the start of fermentation, Lt; / RTI &gt; The citric acid-cinnamon extract according to claim 2, wherein the citric acid-cinnamon extract is prepared by adding 0.015 to 0.025% (w / v) cinnamon slices to a 0.8-1.2 M citric acid solution and extracting the mixture at 70-80 ° C for 48-72 hours Lt; / RTI &gt; The method according to claim 1, wherein the compounding material of step (a) further comprises humus. The microorganism preparation according to claim 4, wherein the microorganism preparation is selected from the group consisting of Bacillus licheniformis , Bacillus subtilus , Bacillus amyloliquefaciens , Cellulomonas uda ), Cellulomonas cellulans cellulans), Clostridium acetonitrile unit Tilikum (Clostridium acetobutylicum , Lactobacillus acidophilus , Lactobacillus &lt; RTI ID = 0.0 &gt; casei ), Lactobacillus bulgaricus , Micrococcus leuteus , Pseudomonas fluorescence , Weisselia cibaria , Issatchenkia &lt; RTI ID = 0.0 &gt; orientalis), Celebi as Saccharomyces My jiae access (Saccharomyces cerevisae , Arspegilus niger , Rhizopus oryzae and Rhizomucor pusillus ) is added to the mixed strains and fixed with sawdust. An organic fertilizer using animal blood, which is a slaughter by-product produced by the method of any one of claims 1 to 5.

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