KR20140007640A - Process for cultivation of culture materials disintegrable organic sludge and culture materials - Google Patents

Abstract

The present invention relates to a method for culturing a culture medium capable of decomposing organic sludge and a culture medium obtained thereby. By the method, a first culture medium is obtained from native microbes in a colony of pine trees and a second culture medium is obtained from soils collected in the respective grounds where Leersia japonica Makino and water dropwort are growing, and then the first and second culture media are fermented and cultured. The culture medium obtained by the method has an ability to decompose various kinds of organic sludge, especially, the organic sludge such as milk sludge or sewage sludge can be decomposed within 20 days at the latest.

Description

Cultivation method of culture with organic sludge resolution and its culture {Process for Cultivation of culture materials disintegrable organic sludge and Culture materials}

The present invention relates to a method for culturing a culture having an organic sludge resolution, and a culture obtained therefrom, and more particularly to a method for culturing a culture having an organic sludge, such as milk sludge or sewage sludge.

Increases in organic waste, such as food waste, sludge from sewage treatment plants, waste from fish processing plants, waste from agricultural and livestock processing plants, garbage from food and paper mills, slaughterhouse slaughterhouses, livestock manure and other organic raw waste Is a major cause of environmental and water pollution.

Accordingly, efforts to compost organic waste have been made in various ways. In general, composting refers to the formation of semi-dry solid organics in a stable form by the biological action of various aerobic microorganisms under aerobic conditions. Composting has the advantages of high organic removal rate due to the reaction at high temperature, stable processing efficiency for organic load, and no nitrification at high temperature, so that efficient use of supplied oxygen can be obtained and stable treatment can be obtained. . Composting requires systematic research on the development of fermentation system and operation technology to improve process efficiency, and the separation of microorganisms including microorganisms and major factors necessary for composting and decomposition carriers, and microorganisms with high enzyme activity.

Various techniques are disclosed relating to the treatment of organic waste or slurries (sludge) by composting microorganisms or compositions comprising microorganisms.

As an example, Korean Patent Registration No. 10-581738 discloses a method for preventing odors caused by incineration or recycling of organic waste such as food waste, pig meal, phosphorus and sewage sludge (sludge) using microorganisms. Specifically, the organic waste storage container is sprayed with a microbial agent, and then the organic waste is collected, and then the microbial agent is added and transported before transporting the organic waste. The preparation is strain SJP6728AF1 deposited with accession no. KCCM-10675P, strain SJP6729AF2 deposited with accession no. KCCM-10677P, strain SJP6730AF3 deposited with accession no. KCCM-10679P, deposit No. SJP6722A5 strain deposited with No. 10676P, SJP6731B1 strain deposited with No. KCCM-10680P. It is material.

As another example, Korean Patent Registration No. 10-0822239 is a mixed microorganism having the ability to strongly decompose fish waste liquid, Bacillus subtilis, Bacillus liqueniformis, Bacillus agri, Bacillus coagulans, Bacillus circulans, Bacillus A mixed microorganism consisting of anthracis and Bacillus pushformis is disclosed.

As another example, Korean Patent Registration No. 10-1098520 describes a microbial agent for composting organic wastes and a method for preparing the same, wherein the activity of the degrading enzymes on starch, fat, protein, and cellulose is high and even at high temperatures. Aneurinibacillus thermophilus ( Aneurinibacillus) as a microbial agent with excellent composting and deodorizing ability thermoaerophilus ) KCCM 41604 microorganisms.

[Patent Literature]

Domestic Patent Registration No. 10-581738

Domestic Patent Registration No. 10-0822239

Domestic Patent Registration No. 10-1098520

In one embodiment of the present invention to provide a method for culturing a culture having excellent resolution for organic waste using bacteria and indigenous plants and extracts can be easily obtained from the surrounding.

One embodiment of the present invention is to provide a culture that has a good resolution for organic waste, in particular milk sludge or sewage sludge can be completely decomposed within at least 20 days.

In one embodiment of the present invention is mixed with indigenous microorganisms collected from the pine colony and fermented rice and bread powder, fermented for 5 to 7 days, here, grass, reeds, dandelion, buttercup leaves, plantain, bellflower, pine needles, pine powder, pine powder , Indongcho, hamcho, mugwort, persimmon grass, deodeok, purslane, pine sulphate fermentation broth, fermented broth and fermented soil were fermented at 20-25 ° C. for 5 days to 7 days, and then added vegetable amino acids and 5 days at 35-55 ° C. Fermenting for 7 to 7 days and incubating at 55 to 60 ° C. for 18 to 21 days to obtain a first culture; The soil collected from the land where the leaflets grow, the soil collected from the land where the buttercups grow, and the soil collected from the rice paddy are mixed with gourd rice and bread flour and fermented for 5 to 7 days. , Fermented makgeolli, auditory, lactic acid bacteria fermented milk, buttercups and napa sashimi, fermented at 20 to 25 ℃ for 5 to 7 days, then added animal amino acids and fermented at 35 to 55 ℃ for 5 to 7 days and 55 to 60 ℃ Incubating for 18 days to 21 days to obtain a second culture; Fermentation of the mixed culture to mix the first culture and the second culture fermentation for 10 to 15 ℃ 5 to 7 days and then fermentation for 5 to 7 days at 55 to 60 ℃; And it provides a method of culturing a culture having an organic sludge decomposition, including a final culture step of culturing the fermentation of the mixed culture for 5 to 7 days.

In the culturing method according to an embodiment of the present invention, 0.9-1 kg of indigenous microorganisms collected from pine communities in the process of obtaining the first culture is mixed with 1.6 to 2.4 kg of soybean rice and 1.6 to 2.4 kg of bread flour, and fermented. 0.8 to 1.2kg grass, reed 0.8 to 1.2kg, dandelion 0.8 to 1.2kg, buttercup 0.8 to 1.2kg, diameter 0.8 to 1.2kg, bellflower 0.8 to 1.2kg, pine needles 1.6 to 2.4kg, pine powder 1.6 to 2.4kg , Pine needles 1.6 to 2.4kg, Indongcho 0.8 to 1.2kg, seaweed 0.8 to 1.2kg, mugwort 0.8 to 1.2kg, green grass 0.8 to 1.2kg, deodeok 0.8 to 1.2kg, purslane, pine needle fermentation 1.6 to 2.4kg, pipe brush extract It may be desirable to mix 1.6-2.4 kg and 0.8-1.2 kg of foliar soil to ferment, then add 400-600 mg of vegetable amino acids.

In the culture method according to an embodiment of the present invention, in the process of obtaining a second culture 0.9 ~ 1kg of soil collected from the land where the leaflets grow, 0.9 ~ 1kg of soil collected from the land where the buttercups grow and collected in paddy fields Ferment by mixing 0.9-1kg of soil with 1.6-2.4kg of soybean rice and 1.6-2.4kg of breadcrumbs, and add 400-600mg of soybean paste, 400-600mg of meju, 400-600mg of soybean soup, 400-600mg of soy sauce, 0.8-1.2kg of kimchi fermentation solution. Makgeolli 1.6-2.4L, hearing 0.8-1.2kg, lactic acid bacterium fermented milk 800-1,200mL, buttercup 0.8-1.2kg and fermentation 0.8 ~ 1.2kg fermented, and then it is preferable to add 900 to 1,000mg of animal amino acid can do.

Another embodiment of the present invention provides a culture having an organic sludge resolution obtained according to the culture method according to the above-described embodiments.

In one embodiment of the present invention, the organic sludge may be milk sludge or sewage sludge.

In one exemplary embodiment of the present invention provides a compost comprising a culture having an organic sludge resolution according to the above embodiments.

The culture method of the culture according to the present invention has the effect of producing a culture having an excellent resolution for organic waste through fermentation techniques using indigenous strains, indigenous plants and extracts can be easily obtained in the vicinity. . The culture obtained therefrom exhibits the effect of degrading organic wastes, especially under aerobic or anaerobic environments, and has the effect of completely degrading milk sludge or sewage sludge within at least 20 days.

Organic waste in the above and the following descriptions means food waste, sludge from sewage treatment plant, waste from fish processing plant, waste from agricultural and livestock processing plant, garbage from food and paper plant, slaughterhouse slaughterhouse, livestock manure and It contains wastes containing other organic substances and can be classified in particular as milk sludge or sewage sludge.

In the method of culturing a culture having an organic sludge resolution according to an embodiment of the present invention, the step of obtaining the first culture is a mixture of indigenous microorganisms collected from pine communities with fermented rice and bread flour and fermented for 5 to 7 days. Pampas grass, reeds, dandelion, buttercups, plantain, bellflower, pine needles, pine pollen, pine needles, Indongcho, hamcho, mugwort, perilla seedlings, deodeok, purslane, pine needles, fermented broth, and pine needles at 20 to 25 ℃ After fermentation for one to seven days, vegetable amino acids are added, fermented at 35 to 55 ° C. for 5 to 7 days, and cultured at 55 to 60 ° C. for 18 to 21 days.

When indigenous microorganisms collected from pine communities are mixed with gourd rice and bread crumbs and fermented for 5 to 7 days, white mold is formed and it turns to blue when it turns blue.

Various plant and soil components are mixed and fermented thereto, and the plant adopted in one embodiment of the present invention mainly includes pine-derived ones, such as pine needles, pine pollen, rosin powder, pine needle fermentation broth, and gwansol extract. .

Pine contains high amounts of amino acids, which are said to be life's best gifts on earth. Pine contains aspartic acid, valine, threonine, methionine, serine, sothocin, glutamic acid, leucine, pcholine, tyrosine, glutin, phenylalanine, and alanine. It contains a large amount of amino acids, such as lysine, cystine, chitinin, and arginine.

Calendula powder contains a large amount of calcium, protein, choline, carbohydrates, and calcium is 6,800 times larger than bamboo shoots and contains a large amount of oil.

Rosin powder is obtained by crushing dried pine rosin from shade.

The pineal fermentation broth is a fermentation broth obtained by taking early spring shoots, blending them with sugar in the same amount, and fermenting them for about 20 to 25 days.

In addition, the gwansol extract is harvested by harvesting the roots and decoction until 100% of the amount of water to the root of 100g root until sticky, then diluted with water to a concentration of 20 to 30% by volume.

Purslane contains tannins, glutins, essential fatty acids omega 3, saponins, calcium and the like, and reeds, dandelions, buttercups, plantains, honeysuckle, seaweed, mugwort and wormwood are hard to die by herbicides. Considering the properties, the first culture is prepared including these plants.

The foliar soil is added in consideration of the multiplication of microorganisms.

Specifically, the preferred blending amount of each component in the process for obtaining the first culture is fermented by mixing 0.9 to 1 kg of native microorganisms collected from pine communities with 1.6 to 2.4 kg of soybean rice and 1.6 to 2.4 kg of breadcrumbs, and 0.8 to 0.8 billion buckwheat grass. 1.2kg, reed 0.8-1.2kg, dandelion 0.8-1.2kg, buttercup leaf 0.8-1.2kg, plantain 0.8-1.2kg, bellflower 0.8-1.2kg, pine needle 1.6-2.4kg, pine powder 1.6-2.4kg, pine powder 1.6 To 2.4kg, Indongcho 0.8 to 1.2kg, seaweed 0.8 to 1.2kg, mugwort 0.8 to 1.2kg, perilla 0.8 to 1.2kg, deodeok 0.8 to 1.2kg, purslane, pine needle fermentation 1.6 to 2.4kg, pipe brush extract 1.6 to 2.4kg And it is preferable in terms of fermentation efficiency to ferment by mixing 0.8 ~ 1.2kg of the foliar soil, it may be desirable to add 400 to 600mg of vegetable amino acids in terms of the multiplication of microorganisms.

In one embodiment of the present invention, a second culture is prepared separately from the preparation of the first culture. The second culture is fermented for 5 to 7 days by mixing the soil collected from the land where the larvae grow, the soil collected from the land where the buttercups grow, and the soil collected from the rice paddy, with soybean rice and bread crumbs, and doenjang, meju, Cheonggukjang, soy sauce, kimchi fermentation broth, makgeolli, auditory, lactic acid bacteria fermented milk, buttercups and naples to ferment for 5 to 7 days at 20 to 25 ℃, and then added animal amino acids and 5 to 7 days at 35 to 55 ℃ It can be obtained by fermentation and incubating for 18 to 21 days at 55 to 60 ℃.

The soil collected from the land where the grass grows, the soil collected from the land where the buttercups grow, and the soil collected from the rice fields may contain various microorganisms, especially anaerobic microorganisms. This is because taking three kinds of soil and fermentation is preferable in terms of obtaining a large amount of microorganisms at once.

These three soils are mixed with gourd rice and breadcrumbs and fermented for 5-7 days.

Doenjang, meju, Cheonggukjang, soy sauce, kimchi fermentation broth, makgeolli, auditory, lactobacillus fermented milk, buttercup, and nachosae are fermented at 20 to 25 ° C. for 5 to 7 days.

The plants of the genus Ibana and Buttercup are hard to die by the spraying of herbicides that have been used conventionally and have been applied to the production of the second culture.

Doenjang, Meju, Cheonggukjang, and soy sauce contain detoxifying ingredients and are traditional Korean berries containing Bacillus bacteria.

Kimchi fermentation liquid may be defined as a liquid in the kimchi fermentation obtained by soaking kimchi and fermentation at a temperature of 20 to 25 ℃ for at least 20 days.

The lactic acid bacteria fermented milk may be a lactic acid bacteria fermented milk which is roughly classified as a commercial yoghurt, and there is no limitation, and may be obtained by fermentation by adding lactic acid bacteria commonly known to milk.

Makgeolli's main ingredient is water, and it also contains carbohydrates, fats, vitamins, 10 kinds of essential amino acids, lactic acid bacteria, and yeast.

Hearing, called seaweed, is the prince of the sea. It catches parasites, eliminates odors, prevents food decay, and contains large amounts of preservatives.

Specifically, in the process of obtaining the second culture, the preferred blending amount of each component is 0.9 to 1 kg of soil collected from the land where the mulberry grows, 0.9 to 1 kg of soil collected from the land where buttercups grow, and 0.9 to 1 kg of soil collected from the paddy field. Fermented by mixing 1.6 to 2.4 kg of soybean rice and 1.6 to 2.4 kg of breadcrumbs, here 400 to 600 mg of soybean paste, 400 to 600 mg of meju, 400 to 600 mg of soybean paste, 400 to 600 mg of soy sauce, 0.8 to 1.2 kg of kimchi fermentation solution, 1.6 to 1.6 rice wine. It is preferable to ferment by mixing 2.4 L, hearing 0.8 to 1.2 kg, lactic acid bacterium fermented milk 800 to 1,200 mL, buttercup 0.8 to 1.2 kg and 0.8 to 1.2 kg of Namiaceae, which is added to the amount of 900 to 1,000 mg of animal amino acids. It is preferable.

In the above and the following description, vegetable amino acids and animal amino acids include all fertilizers or nutrients for amino acid supply known in plant cultivation.

The first culture and the second culture obtained as described above are mixed and fermented at 10 to 15 ° C. for 5 to 7 days and then fermented at 55 to 60 ° C. for 5 to 7 days (for convenience, 'mixed culture of Fermentation process').

Singularity in the fermentation process of the mixed culture is that the fermentation at a low temperature and the fermentation at a high temperature, thereby allowing the culture obtained from an embodiment of the present invention to express the degradation effect in any environment.

Finally, by culturing the fermentation of the mixed culture for 5 to 7 days (for convenience, referred to as the 'final culture process') it can be prepared a culture having an organic sludge decomposition according to one embodiment of the present invention. At this time, the temperature condition of the culture is preferably 40 to 55 ℃.

The culture obtained through such a series of processes is excellent in organic sludge resolution, it was confirmed in one embodiment is particularly excellent in the resolution of milk sludge or sewage sludge.

Therefore, the culture of the present invention can be expected to be useful in the production of compost containing organic waste.

Hereinafter, the present invention will be described in detail with reference to the following examples, which are not to be construed as limiting the present invention thereto.

Example

(1) Preparation of the First Culture

Indigenous microorganisms were collected from deep mountain pine communities. 1kg of the collected indigenous microorganisms were mixed with 2kg of gourd rice and 2kg of bread crumbs and left for 1 week at room temperature to form white mold. If white mold turns blue, here, 1 kg of pampas grass, 1 kg of reeds, 1 kg of dandelion, 1 kg of buttercup leaves, 1 kg of plantain, 1 kg of bellflower, 2 kg of pine needles, 2 kg of pine pollen, 2 kg of pine needles, 1 kg of indongcho, 1 kg of seaweed, 1 kg of mugwort , 1kg perilla, 1kg deodeok, 1kg of purslane, 2kg of pineal fermentation broth, 2kg of tubular extract and fermented at room temperature for 1 week.

500 mg of vegetable amino acids were mixed in the fermentation, and fermented at 35 to 55 ° C. for 1 week, and then cultured at 55 to 60 ° C. for 3 weeks to obtain a first culture.

(2) Preparation of the Second Culture

1 kg of soil in the land where the leaflets grow, 1 kg of soil in which buttercups grow, and 1 kg of paddy soil were mixed with 2 kg of soybean rice and 2 kg of breadcrumbs and fermented at room temperature for 1 week.

Fermented soybean paste 500mg, Meju 500mg, Cheonggukjang 500mg, Soy sauce 500mL, Kimchi fermentation solution 1kg, Makgeolli 2L, hearing 1kg, lactic acid bacteria fermented milk 1,000mL, buttercup 1kg, Nagyeopsal 1kg and fermented at room temperature for 1 week. 1,000 mg of the animal amino acid was added to the fermentation, and fermented for one week at 35 to 55 ° C., which was incubated at 55 to 60 ° C. for three weeks to obtain a second culture.

(3) Preparation of fermented product of mixed culture

The first culture and the second culture were mixed and fermented at 10 to 15 ° C. for 1 week, which was again fermented at 55 to 60 ° C.

(4) final culture

The fermented product of the mixed culture obtained in the above (3) was incubated for 1 week at 40 to 55 ℃ to obtain a final culture.

The final culture thus obtained was tested as follows to confirm that the organic sludge had a resolution.

Experiment 1: Milk Sludge Decomposition

50kg of milk sludge which is a wastewater treatment sludge obtained from Maeil Dairy Co., Ltd. Gwangju factory, 5kg of culture obtained from the above example, 6kg of mushroom waste medium, 5kg of bovine soil, 25kg of chaff, 8kg of sawdust, 1kg of charcoal powder 500g Fermentation in the state.

On the first day of the fermentation, the sludge temperature rose to 40 ° C., rose to 50 ° C. on day 2, and to 55 ° C. on day 3. On the 5th day, the 1st turn over and after that, the temperature rise was confirmed, and from the 9th day, the temperature began to fall, and on the 10th, the 2nd flipped, and on the 12th day, the temperature rose to 65 ℃, It was confirmed that it was completely removed.

This confirmed that the milk sludge was completely decomposed on day 12.

A summary of the specific milk sludge decomposition process is shown in Table 1 below.

Elapsed days Contents 0 Sludge and Material Mixture One Start of fermentation, sludge temperature 40 ℃ 2 Temperature increase 50 ℃ 3 Temperature rise 55 ℃ 4 Slightly lack of moisture due to temperature rise 5 1st turn over, water and sawdust supply, more than 60% decomposed 6 Temperature zoom 60 ℃ 7 Temperature rise 65 ℃ 8 Temperature rise 65 ℃ 9 Temperature drop 55 ℃ 10 45 ° C, 90% Degraded, 2nd flip 11 Temperature rise 65 ℃, odor completely removed 12 100% decomposition

Experiment 2: Milk Sludge Decomposition

100 kg of milk sludge which is a wastewater treatment sludge obtained from Maeil Dairy Co., Ltd. Gwangju factory, 10 kg of culture obtained from the above example, 12 kg of mushroom waste medium, 10 kg of foliar soil, 10 kg of humus, 50 kg of chaff, 16 kg of sawdust, 1 kg of bean curd and 1 kg of charcoal flour were mixed and fermented at room temperature.

On the first day of the fermentation, the sludge temperature rose to 65 ℃ and the first inversion was performed on the fourth day, and after that, the temperature was increased and the temperature began to fall from the 9th day, and the second inversion on the tenth day, On day 19, it was confirmed that milk sludge was completely decomposed. Check the temperature change every day to check the degree of fermentation.

The specific milk sludge decomposition process is summarized in Table 2 below.

Elapsed days Contents 0 Sludge and Material Mixture One Temperature increase 65 ℃ 2 Temperature 65 ℃ 3 Slightly lowered to 60 ℃ 4 1st flip 5 Slight moisture, 63 ℃ 6 Temperature 65 ℃ 7 Slightly lowered to 60 ℃ 8 Temperature rise to 63 ℃ 9 Temperature rise to 65 ℃ 10 2nd flip, add moisture and sawdust 11 Temperature 55 ℃ 12 Temperature rise to 64 ℃ 13 Temperature rise to 65 ℃ 14 3rd inverted water and sawdust added 15 Temperature 55 ℃ 16 Temperature 55 ℃ 17 Temperature drops to 50 ℃ 18 Lowered to 45 ℃ 19 100% decomposition

Experiment 3: Sewage Sludge Decomposition

2 tons of sewage sludge obtained from Haenam-gun Office, culture 200kg obtained from the above example, 240kg mushroom waste medium, 200kg of foliar soil, Chaff 10kg, sawdust 320kg, bean curd 40kg charcoal powder 20kg was mixed and fermented at room temperature.

On the first day of the fermentation, the sludge temperature rose 32 ° C and the temperature gradually increased, inverting the first one on the sixth day, inverting the second on the eleventh day, and inverting the third on the 16th day. On day 21, the temperature was stabilized to 30 ° C., and the sludge was completely decomposed as leaven. Check the temperature change every day to check the degree of fermentation.

To summarize the specific sewage sludge decomposition process is shown in Table 3.

Elapsed days Contents 0 Sludge and Material Mixture One Temperature 32 ℃ 2 Temperature 35 ℃ 3 Temperature 40 ° C 4 Temperature 50 ℃ 5 Temperature 55 ℃ 6 1st flip 7 Temperature 60 ℃ 8 Temperature 55 ℃ 9 Temperature 50 ℃ 10 Temperature 50 ℃ 11 Temperature 43 ℃, 2nd flip 12 Temperature 45 ℃ 13 Temperature 45 ℃ 14 Temperature 45 ℃ 15 Temperature 40 ° C 16 3rd flip, mix mushroom waste medium and sawdust 17 Temperature 45 ℃ 18 Temperature 50 ℃ 19 Temperature 48 ℃ 20 Temperature 40 ° C 21 Temperature 30 ℃, fermentation complete

Claims (6)

Indigenous microorganisms collected from pine communities are mixed with gourd rice and bread powder and fermented for 5 to 7 days, followed by silver grass, reeds, dandelions, buttercups, plantains, bellflowers, pine needles, pine pollen, pine needles, indongcho, hamcho, mugwort, Perilla seedlings, deodeok, purslane, sol-fermented fermentation broth, pipe brush extract, and foliar soil are fermented at 20 to 25 ° C. for 5 days to 7 days, and then the vegetable amino acid is added and fermented at 35 to 55 ° C. for 5 days to 7 days, and 55 to Culturing at 60 ° C. for 18 to 21 days to obtain a first culture;
The soil collected from the land where the leaflets grow, the soil collected from the land where the buttercups grow, and the soil collected from the rice paddy are mixed with gourd rice and bread flour and fermented for 5 to 7 days, and here, doenjang, meju, cheonggukjang, soy sauce and kimchi. , Fermented makgeolli, auditory, lactic acid bacteria fermented milk, buttercups and napa sashimi, fermented at 20 to 25 ℃ for 5 to 7 days, then added animal amino acids and fermented at 35 to 55 ℃ for 5 to 7 days and 55 to 60 ℃ Incubating for 18 days to 21 days to obtain a second culture;
Fermentation of the mixed culture to mix the first culture and the second culture fermentation for 10 to 15 ℃ 5 to 7 days and then fermentation for 5 to 7 days at 55 to 60 ℃; And
A method of culturing a culture having an organic sludge resolution, including a final culture step of culturing the fermented product of the mixed culture for 5 to 7 days. The method according to claim 1, wherein 0.9 to 1 kg of native microorganisms collected from pine communities in the process of obtaining the first culture is fermented by mixing 1.6 to 2.4 kg of soybean rice and 1.6 to 2.4 kg of breadcrumbs, 0.8 to 1.2 kg of silver grass grass, Reed 0.8-1.2kg, Dandelion 0.8-1.2kg, Buttercup 0.8-1.2kg, Plantain 0.8-1.2kg, Bellflower 0.8-1.2kg, Pine needles 1.6-2.4kg, Pine powder 1.6-2.4kg, Pine powder 1.6-2.4kg , Indong vinegar 0.8 to 1.2kg, seaweed 0.8 to 1.2kg, mugwort 0.8 to 1.2kg, green grass 0.8 to 1.2kg, deodeok 0.8 to 1.2kg, purslane, pine needle fermentation broth 1.6-2.4kg, pipe brush extract 1.6-2.4kg and leafy soil 0.8 To 1.2 kg of mixed fermentation, and then 400 to 600 mg of vegetable amino acids. The method according to claim 1, wherein 0.9 to 1 kg of soil collected from the land where the mulberry grows in the process of obtaining the second culture, 0.9 to 1 kg of soil collected from the land where buttercups grow, and 0.9 to 1 kg of soil collected from the rice paddy Fermented by mixing with 2.4 to 2.4 kg and 1.6 to 2.4 kg of breadcrumbs, including 400 to 600 mg of soybean paste, 400 to 600 mg of meju, 400 to 600 mg of soybean soup, 400 to 600 mg of soy sauce, 0.8 to 1.2 kg of kimchi, 1.6 to 2.4 L of rice wine, and hearing 0.8 to 1.2 kg, lactic acid bacteria fermented milk 800 to 1,200 mL, buttercup 0.8 to 1.2 kg and Naples 0.8 to 1.2 kg of fermentation and fermentation, and then cultured with organic sludge resolution, characterized in that the addition of 900 to 1,000 mg of animal amino acids Cultivation method of water. A culture having an organic sludge resolution obtained according to the culture method according to any one of claims 1 to 3. The culture according to claim 4, wherein the organic sludge is milk sludge or sewage sludge. Compost comprising the culture having the organic sludge resolution of claim 4.