CN106635902A - Bacillus coagulans and application thereof - Google Patents
Bacillus coagulans and application thereof Download PDFInfo
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Abstract
The invention relates to bacillus coagulans which is preserved in China Center For Type Culture Collection (CCTCC) and has the preservation number of CCTCC M2016440, and application of the bacillus coagulans to a digestion aspect of livestock. The bacillus coagulans has a high lactic acid production capability, promotes digestion on feed of the livestock, and can be applied to the aspect of feeding the livestock. The bacillus coagulans can secrete a lot of lactic acid, thereby effectively inhibiting the growth of harmful bacteria, improving an intestine microenvironment, promoting intestine development, enhancing intestine functions and promoting digestion and absorption on the feed of the livestock. Meanwhile, the bacillus coagulans has good environment stress resistance, can be used for effectively inhibiting the growth of the harmful bacteria, rapidly reducing ammonia gas in the environment and improving a breeding environment, and can be used for carrying out harmless treatment on corpses of the livestock, and deodorization and bacterium inhibition of a livestock farm. The invention further provides a composition for harmlessly treating the corpses of the livestock and a composition for deodorizing and inhibiting bacteria of the livestock farm.
Description
Technical field
The present invention relates to microbe application field, more particularly to a kind of Bacillus coagulans and its application.
Background technology
Bacillus coagulans (Bacillus coagulans), Grain-positive belongs to hard (or thick) wall bacterium door.Condense spore
Belong to bacillus on bacillus taxonomy, cell is in shaft-like, gram positive bacteria, terminal spore, atrichia.Decompose saccharide life
It is homofermentative lactic bacterium into Pfansteihl.Optimum growth temperature is 45-50 DEG C, and optimum pH is 6.6-7.0.Bacillus coagulans
Be facultative anaerobic bacteria, all can grow in the environment of aerobic and anaerobic, adapt to the intestinal environment of hypoxia, to acid and bile have compared with
High toleration, can carry out lactate fermentation, and the Pfansteihl of generation can reduce gut pH, suppress harmful bacteria, and can promote double
The growth and breeding of the probioticss such as discrimination bacillus.Bacillus coagulans can form spore, the bacillus cereuss with other not lactic acid producings
Compare and be conducive to recovering gastrointestinal microecological balance.
At present, additive for microbe feedstuff be using Ministry of Agriculture's accreditation beneficial microbe it is fermented made by biological system
Agent, is the best substitute of antibiotic.It is adjustable for improving poultry, the artificial culture bacterium of the health level of fowl and its metabolite
Microbial state treatment in whole poultry, carcass, keeps microecological balance, is to excite animal own beneficial culture propagation to increase, while supporting
It is formed with the microbial product of harmful fungus strain growth.Additive for microbe feedstuff exist subject matter be excellent species selection and by
Additive for microbe feedstuff activity is reduced caused by strain inactivation.
China is domestic animal cultivation big country, while animal husbandry development, daily because of natural death due to animal feeding-stuff radix greatly
Dead livestock number is quite huge with disease.According to animal dead reason, the source of spoil can be divided into natural selection
Or natural death, the dead two kinds of forms of disease.Natural death is eliminated and endangers less, because of the improper process of spoil that epidemic disease is dead
It is not only serious to water source, soil, atmospheric pollution, it is also easy to cause the propagation of epidemic disease and spreads.With foot and mouth disease, swine fever, high cause
Characteristic of disease bird flu etc. has strong communicable epidemic-infected animal corpse, or even the life and health for seriously threatening the mankind.Traditional
Animal corpse process mainly has landfill method, sterilization, burning method, change preparation method, biological degradation method (fermentation method).Wherein, using life
Thing edman degradation Edman processes spoil, but processes the overlong time or foul smelling generation of corpse.
The content of the invention
The present inventor is by the research to Bacillus coagulans, screening and verifies, there is provided a kind of lactic acid producing ability is strong
Bacillus coagulans, the Bacillus coagulans strain and the compositionss containing its composition, can effectively apply to the life of feed additive
Produce, plant's deodorization is antibacterial, dead livestock and poultry corpse harmless treatment.
For the present invention first goal of the invention, the invention provides being preserved in China typical culture collection center
(CCTCC), preservation address is：Wuhan City, Hubei Province, Hongshan District Bayi Road, Wuhan University；Classification And Nomenclature is：Bacillus coagulans
No. 1, Bacillus Coagulans 1；Deposit number is：CCTCC M 2016440, preservation date is：On September 1st, 2016
Bacillus coagulans.The lactic acid producing ability of the Bacillus coagulans of the present invention is strong, can promote digestion of the poultry to feedstuff.
To reach second goal of the invention of the present invention, the present invention is provided and is preserved in CCTCC, and preserving number is CCTCC M
Application of 2016440 Bacillus coagulans in terms of livestock rearing.In feeding process, add in animal feed or drinking-water
CCTCC is preserved in, preserving number is the Bacillus coagulans of CCTCC M 2016440.
CCTCC is preserved in, preserving number can secrete a large amount of lactic acid for the Bacillus coagulans of CCTCC M2016440, effectively suppression
The growth of harmful bacteria processed, improves intestine microenvironment, promotes intestinal growth, strengthens function of intestinal canal, promotes poultry to feedstuff
Digest and assimilate.
To reach the 3rd goal of the invention of the present invention, the present invention provides a kind of additive for microbe feedstuff, micro- life
Containing being preserved in CCTCC in thing feed additive, preserving number for CCTCC M2016440 Bacillus coagulans as effectively into
Point.
CCTCC is preserved in, preserving number can secrete a large amount of lactic acid for the Bacillus coagulans of CCTCC M2016440, effectively suppression
The growth of harmful bacteria processed, improves intestine microenvironment, promotes intestinal growth, strengthens function of intestinal canal, promotes poultry to feedstuff
Digest and assimilate.
To reach the 4th goal of the invention of the present invention, the present invention provides a kind of plant's deodorization antibacterial compositionss, institute
State containing CCTCC is preserved in compositionss, preserving number is the Bacillus coagulans of CCTCC M2016440 as effective ingredient.
Further, the antibacterial compositionss of plant's deodorization, including 50 weight portion preserving numbers are for CCTCC M2016440's
Bacillus coagulans, 100 weight portion preserving numbers are the bacillus subtilises of CCTCC M 2016441,100 weight portion preserving numbers are
The bacillus subtilises of CCTCC M 2016443 and the montmorillonite powder of 750 weight portions.Wherein, preserving number is CCTCC
The viable count of the Bacillus coagulans of M2016440 is 1.0 × 109CFU/g～5.0 × 109CFU/g, preserving number is CCTCC M
The viable count of 2016441 bacillus subtilises is 2.0 × 109CFU/g～1.0 × 1010CFU/g, preserving number is CCTCC M
The viable count of 2016443 bacillus subtilises is 5.0 × 108CFU/g～5.0 × 109CFU/g。
The preserving number is the bacillus subtilises of CCTCC M 2016441, and Classification And Nomenclature is：Bacillus subtilises No. 1,
Bacillus Subtilis 1；China typical culture collection center (CCTCC) is preserved in, preservation address is：Hubei Province is military
Chinese city, Hongshan District Bayi Road, Wuhan University；Preservation date is：On September 1st, 2016.
The preserving number is the bacillus subtilises of CCTCC M 2016443, and Classification And Nomenclature is：Bacillus subtilises No. 3,
Bacillus Subtilis 3, are preserved in China typical culture collection center (CCTCC), and preservation address is：Hubei Province is military
Chinese city, Hongshan District Bayi Road, Wuhan University；Preservation date is：On September 1st, 2016.
CCTCC is preserved in, preserving number has excellent environment resistance for the Bacillus coagulans of CCTCC M2016440,
The growth of harmful bacteria, the quick ammonia reduced in environment is effectively suppressed to improve breeding environment.
To reach the 5th goal of the invention of the present invention, the present invention provides a kind of group for poultry corpse harmless treatment
Compound, containing CCTCC is preserved in the compositionss, preserving number is the Bacillus coagulans of CCTCC M2016440 as effective
Composition.
Further, for the compositionss of poultry corpse harmless treatment, including 100 weight portion preserving numbers are CCTCC
The Bacillus coagulans of M2016440,100 weight portion preserving numbers are bacillus subtilises, 100 weight of CCTCC M 2016441
Part preserving number is the bacillus subtilises of CCTCC M 2016443 and the zeolite powder of 700 weight portions.Wherein, preserving number is CCTCC
The viable count of the Bacillus coagulans of M2016440 is 2.0 × 108CFU/g～1.0 × 109CFU/g, preserving number is CCTCC M
The viable count of 2016441 bacillus subtilises is 3.0 × 109CFU/g～1.0 × 1010CFU/g, preserving number is CCTCC M
The viable count of 2016443 bacillus subtilises is 1.0 × 109CFU/g～1.0 × 1010CFU/g.It is preserved in CCTCC, preservation
Number there is excellent environment resistance for the Bacillus coagulans of CCTCC M2016440, effectively suppress the growth of harmful bacteria, can
Organic acid reaction is efficiently produced, the quick ammonia reduced in environment improves environment.
Specific embodiment
To describe the technology contents of technical scheme, structural features in detail, purpose and effect being realized, below in conjunction with concrete reality
Apply example to be explained in detail.
CCTCC is preserved in, preserving number is that inventor trains in solid fermentation for the Bacillus coagulans of CCTCC M2016440
When foster, Bacillus coagulans strain produce variation, the separation screening from culture and go out, the bacterial strain is than ATCC (American Type cultures
Collection) 7050 type strains more than 1 times of fast growth, lactic acid producing ability is strong.
The strain cell is shaft-like, single, paired, the micro- moistening in bacterium colony surface, tarnish, opaque without projection, flat, is in
White；Middle micro- thick rim is relatively thin, edge expansion, circular or spread waviness, irregular shape；In culture medium nexine：Bacterium colony
Form is in point-like, around there is transparent circle；Examine under a microscope：Cell is shaft-like, two ends blunt circle, there is spore, and the life of spore end is without whip
Hair；Endospore is formed, Gram-positive (G+), amphimicrobian can ferment and produce a large amount of antibacterial coaguins, lactic acid, ammonia
Base acid, vitamin and various digestive enzyme.The Bacillus coagulans produce lactic acid ability by force, can promote digestion of the poultry to feedstuff.
In separation screening, sample used is oneself of the type strains of ATCC (American type culture collection) 7050
Variant, used medium is selected to press：1000ml distilled water, yeast extract powder 2.5g, peptone 5.0g, glucose 1.0g, tween
80 1.0g, L-Cysteine 0.1g, bromocresol purple 0.06g, the recipe configuration of agar 15.0g, pH is controlled 7.0.
Dilution is configured in the distilled water of 1000ml with quality percent by volume 0.85%NaCl, 0.05% Tween 80 molten
Liquid.Dilute solution dilute sample is used, sample liquid is obtained.
Sample liquid is coated on after above culture medium, in 37 DEG C of anaerobic culture box, after cultivating 72 hours, growing way is selected good
The good, bacterium colony that colonial morphology is similar and number is most, isolates and purifies until the consistent single bacterium of colonial morphology.
The single bacterium isolated is carried out being tested in lactate detection test, lactic acid production highest list bacterium is filtered out.Should
Under micro- aerobic condition, when glucose content is 5.00g/L concentration, it is 1.50g/L to produce acid amount to bacterial strain.The strain for filtering out is guarantor
CCTCC is hidden in, preserving number is the Bacillus coagulans of CCTCC M 2016440.
The fungi preservation for filtering out, will in a dormant state in sand pipe, lyophilization pipe.Need to be lived when using
Change, cultivate, obtain a number of purebred bacterium.
The cultural method of Bacillus coagulans, comprises the steps：
A, actication of culture：Production strain in a dormant state is accessed into test tube slant activation
B, strain expanded culture：Strain is sequentially passed through into first order seed shaking table culture, secondary seed tank culture, three-level seed
Tank culture, fermentation tank are enlarged culture.
The strain expanded culture includes following component with culture medium：Corn starch, bean cake powder, Semen Maydis pulp, yeast extract,
K2HPO4, peptone, MgSO4.7H2O；The pH value of the culture medium is 7.0.
Test in the chicken farm of embodiment 1
This test is randomly divided into 3 groups, matched group 1, matched group 2 and test group from the extra large blue brown laying hen 720 of 31 week old
Each 240, experimental period is 21 days.Test daily ration：Semen Maydiss 61.5%, bean cake 23.4%, Testa Tritici 2%, stone powder 8.1% is combined pre-
Batch mixing 5%, does not feed health-related antibiotic during test.
Matched group 1 is not added with bacterium, and other feeding conditions are identical；Matched group 2 and test group are added during testing in feedstuff
The additive for microbe feedstuff of weight portion 0.1 ‰.Wherein the additive for microbe feedstuff of matched group 2 includes that 1% preserving number is CCTCC
(viable count is 1.0 × 10 to the bacillus subtilises of M20164429Cfu/g) and 99% glucose, test group microbiological feed adds
Plus agent by 1% preserving number for CCTCC M2016442 bacillus subtilises (viable count be 1.0 × 109Cfu/g), 0.1% protect
(viable count is 1.0 × 10 to hide number Bacillus coagulans for being CCTCC M20164408Cfu/g), 98.9% glucose group into.
Result of the test is as follows：
Result of the test shows, single egg size of test group, laying rate and matched group no significant difference, but its standard deviation is more steady
It is fixed, show that adding preserving number can improve the stability that chicken is laid eggs for the Bacillus coagulans of CCTCC M2016440.In feedstuff profit
With aspect, than the reduction of matched group 1 5.2%, feedstuff-egg ratio reduces by 4.9% to test group daily material consumption, and its effect is more brighter than matched group
It is aobvious.As a result show, on the basis of preserving number is for the bacillus subtilises of CCTCC M2016442, addition preserving number is CCTCC
The Bacillus coagulans of M2016440, can strengthen stability of laying eggs, and improve efficiency of feed utilization.
The preserving number is the bacillus subtilises of CCTCC M 2016442, and Classification And Nomenclature is：Bacillus subtilises No. 2,
Bacillus Subtilis 2；China typical culture collection center (CCTCC) is preserved in, preservation address is：Hubei Province is military
Chinese city, Hongshan District Bayi Road, Wuhan University；Preservation date is：On September 1st, 2016.
The nursery pig feeding experiment of embodiment 2
On pig farm, nursery house randomly selects 3 hurdle nursery pig, and per 20, hurdle, wherein 2 hurdles are matched group, 1 hurdle is used as test group.
Matched group 1 feeds chlortetracycline for health care, acidulant and sweet using the feedstuff without microbe additive during test
Taste agent.
Matched group 2 adds 0.1% additive for microbe feedstuff in feedstuff, and additive for microbe feedstuff is by 10g hays
(preserving number is the bacillus subtilises of CCTCC M2016442 to bacillus cereuss, and viable count is 1.0 × 109Cfu/g), the Portugal of 990g
Grape sugar is mixed to prepare.Chlortetracycline for health care, acidulant and sweeting agent are not used during test.
Test group adds the additive for microbe feedstuff of weight portion 0.1% in feedstuff, and additive for microbe feedstuff is by 1g
Preserving number for CCTCC M2016440 Bacillus coagulans (viable count be 1.0 × 108Cfu/g), 10g preserving numbers are CCTCC
(viable count is 1.0 × 10 to the bacillus subtilises of M20164429Cfu/g), the glucose of 989g is mixed to prepare.During test not
Using chlortetracycline for health care, acidulant and sweeting agent.
After feeding nursery pig 31 days, nursery pig is detected, each group is as a result as shown in the table without death：
Result of the test shows that the average daily gain amount of test group is higher than matched group 1 and matched group 2, the average day of test group
, without significant difference, less than matched group 1, test group feedstuff-meat ratio is less than matched group 1 and matched group 2 for feed intake and matched group.And in examination
During testing, the stool odor that test group is discharged is substantially low than matched group 1 and matched group 2, it is possible to decrease in breeding process in colony house
Stink.
A kind of antibacterial compositionss of the plant's deodorization of embodiment 3
With the Bacillus coagulans that 50 weight portion preserving numbers are CCTCC M2016440, (viable count is 2.0 × 109cfu/
G), 100 weight portion preserving numbers for CCTCC M2016441 bacillus subtilises (viable count be 1 × 1010Cfu/g), 100 weight
(viable count is 1 × 10 for the bacillus subtilises of CCTCC M2016443 for part preserving number9Cfu/g), take off with the illiteracy of 750 weight portions
Stone powder mix homogeneously, is configured to the antibacterial compositionss of plant's deodorization.
The pig house deodorization bacteriostatic test of embodiment 4
It is test object to choose four pig houses on pig farm, and pig house 1 is matched group 1, and pig house 2 is matched group 2, and pig house 3 is right
According to group 3, pig house 4 is respectively have 1000 or so growing and fattening pigs in test group, four pig house areas and space size all same, pig house.
Matched group 1 is sprayed using clear water；
Matched group 2 is that (viable count is 1 × 10 to CCTCC M2016441 bacillus subtilises using 100g preserving numbers10cfu/
G) with 900g montmorillonite powder mix homogeneously, it is dissolved in the control solvent 2 of 100L clear water preparation；
Matched group 3 adopt 100g preserving numbers for CCTCC M2016441 bacillus subtilises (viable count be 1 ×
1010Cfu/g), 100g preserving numbers for CCTCC M2016443 bacillus subtilises (viable count be 1 × 109Cfu/g), with
800g montmorillonite powder mix homogeneously, is dissolved in the control solvent 3 of 100L clear water preparation.
Test group is dissolved in the survey of 100L clear water preparation using antibacterial compositionss 1kg of plant's deodorization that embodiment 3 is configured
Examination solvent.
With aerosol apparatus deodorized solvent is uniformly sprayed on the ground of pig house, wall, foul smell, ammonia gas are carried out after 5 days
Concentration Testing, it is as a result as shown in the table：
| Stink clearance | Ammonia removal rate | |
| Matched group 1 | 0 | 0 |
| Matched group 2 | 80% | 50% |
| Matched group 3 | 80% | 50% |
| Test group | 90% | 80% |
Wherein, odor concentration adopts triangle odor bag method (GB/T14675), ammonia to adopt sodium hypochlorite-salicylic acid
Spectrophotography (GB/T14679).
Test carries out coliform plate count, Sampling Area after 5 days to pig house feeding area, defecation area and swinery wall
25cm2.As a result such as following table：
| Detection zone | Matched group 1 | Matched group 2 | Matched group 3 | Test group |
| Feeding area | 1.5×106CFU | 3.3×105CFU | 1.6×103CFU | 1.3×103CFU |
| Defecation area | 6.1×106CFU | 9.7×105CFU | 3.2×104CFU | 4.7×104CFU |
| Swinery wall | 3.9×105CFU | 5.2×104CFU | 8.7×102CFU | 6.4×102CFU |
Result of the test shows：Test group antibacterial addition preserving number is the Bacillus coagulans of CCTCC M2016440, with
Matched group compares the ammonia that can obviously reduce environment, meanwhile, add the bacterium has certain suppression to make to the coliform in environment
With.
A kind of compositionss for poultry corpse harmless treatment of embodiment 5
100 weight portion preserving numbers for CCTCC M2016440 Bacillus coagulans (viable count be 1.0 × 109Cfu/g),
100 weight portion preserving numbers for CCTCC M2016441 bacillus subtilises (viable count be 1.0 × 1010Cfu/g), 100 weight
Part preserving number for CCTCC M2016443 bacillus subtilises (viable count be 2.0 × 109Cfu/g), with 700 weight portions
Zeolite powder mix homogeneously, makes a kind of compositionss for poultry corpse harmless treatment.
The poultry corpse harmless treatment of embodiment 6
40 die of illness chicken and 20 dead pig corpse are chosen, is divided into 4 parts of phase homogenous quantities, wherein 3 parts is matched group, 1 part is
Test group, per part adds by sawdust, the substrate composed 5 cubic metres of bedding and padding of rice husk.
Matched group 1 adds 1000g zeolite powders in bedding and padding；
Matched group 2 adds the bacillus subtilises that 100g preserving numbers are CCTCC M2016441 in bedding and padding, and (viable count is
1.0×1010Cfu/g) and 900g zeolite powder；
Matched group 3 adds the bacillus subtilises that 100g preserving numbers are CCTCC M2016441 in bedding and padding, and (viable count is
1.0×1010Cfu/g) and 100g preserving numbers for CCTCC M2016443 bacillus subtilises (viable count be 2.0 × 109cfu/
G) with 800g zeolite powders；
Test group adds a kind of combination for poultry corpse harmless treatment that reality 1000g applies the preparation of example 5 in bedding and padding
Thing.
Sampling and testing after the completion of compost fermentation, the detection of matched group 1 contains Salmonella, fowl plague, swine fever virus testing result
For feminine gender.Test group, matched group 3 and matched group 2 do not detect Salmonella, and fowl plague, swine fever virus testing result are feminine gender.
Matched group 1 needs to complete within 26 days fermentation altogether, and sweat produces foul smell；Matched group 2 needs to complete within 10 days fermentation, but pads
Oils and fatss present in material are only a small amount of to be decomposed, and has ammonia stink and a small amount of foul smell to produce in sweat；Matched group 3 needs to complete for 10 days
Fermentation, oils and fatss decompose in bedding and padding after fermentation, non-greasy, and sweat has ammonia stink and a small amount of foul smell to produce；And test group 10
It completes fermentation, and bedding and padding non-greasy after fermentation, sweat is nearly free from foul smell and ammonia.
As a result show to add Bacillus coagulans of the preserving number for CCTCC M2016440, significantly reduce the row of ammonia
Put, improve the comfort level in artificial operating process.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposit between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant are intended to
Nonexcludability is included, so that a series of process, method, article or terminal unit including key elements not only includes those
Key element, but also including other key elements being not expressly set out, or also include for this process, method, article or end
The intrinsic key element of end equipment.In the absence of more restrictions, limited by sentence " including ... " or " including ... "
Key element, it is not excluded that also there is other key element in the process including the key element, method, article or terminal unit.This
Outward, herein, " it is more than ", " being less than ", " exceeding " etc. are interpreted as not including this number；" more than ", " below ", " within " etc. understand
It is to include this number.
Although being described to the various embodiments described above, those skilled in the art once know basic wound
The property made concept, then can make other change and modification to these embodiments, so embodiments of the invention are the foregoing is only,
Not thereby the scope of patent protection of the present invention, equivalent structure or equivalent process that every utilization description of the invention is made are limited
Conversion, or is directly or indirectly used in other related technical fields, be included in the same manner the present invention scope of patent protection it
It is interior.
Claims (7)
1. CCTCC is preserved in, and preserving number is the Bacillus coagulans of CCTCC M 2016440.
2. CCTCC is preserved in, and preserving number is application of the Bacillus coagulans of CCTCC M2016440 in terms of livestock rearing.
3. a kind of additive for microbe feedstuff, it is characterised in that containing described in claim 1 in the additive for microbe feedstuff
Bacillus coagulans as effective ingredient.
4. a kind of compositionss antibacterial for plant's deodorization, it is characterised in that containing described in claim 1 in the compositionss
Bacillus coagulans as effective ingredient.
5. compositionss antibacterial for plant's deodorization according to claim 4, it is characterised in that including claim 1
Described Bacillus coagulans, preserving number for CCTCC M 2016441 bacillus subtilises and preserving number be CCTCC M
2016443 bacillus subtilises.
6. a kind of compositionss for poultry corpse harmless treatment, it is characterised in that contain claim 1 in the compositionss
Described Bacillus coagulans are as effective ingredient.
7. compositionss for poultry corpse harmless treatment according to claim 6, it is characterised in that will including right
The Bacillus coagulans described in 1, the bacillus subtilises that preserving number is CCTCC M 2016441 and preserving number is asked to be CCTCC M
2016443 bacillus subtilises.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107400637A (en) * | 2017-02-24 | 2017-11-28 | 上海道多生物科技有限公司 | A kind of bacillus coagulans and its microorganism formulation, preparation method and application |
| CN107868763A (en) * | 2017-11-21 | 2018-04-03 | 青岛根源生物技术集团有限公司 | One plant is improved and attacks the bacillus coagulans E21 of the anti-oxidation stress ability of livestock and poultry animal and its application after poison |
| CN108102967A (en) * | 2018-01-11 | 2018-06-01 | 天津生机集团股份有限公司 | One breeder source coagulating bacillus strain and its production spore method |
| CN110699298A (en) * | 2019-11-14 | 2020-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus coagulans P1-678 strain for producing urease inhibitor and application thereof |
| CN111826323A (en) * | 2020-08-05 | 2020-10-27 | 福建洛东生物技术有限公司 | Bacillus subtilis, preparation and application thereof |
| CN112048448A (en) * | 2020-07-27 | 2020-12-08 | 天津开发区坤禾生物技术有限公司 | Complex microbial inoculant for reducing ammonia odor of henhouse and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101638626A (en) * | 2009-09-10 | 2010-02-03 | 惠飞 | Bacillus subtilis and application thereof in biological deodorant |
| CN101665773A (en) * | 2008-12-23 | 2010-03-10 | 中国农业科学院农业环境与可持续发展研究所 | Microbial bacterial agent, preparation method thereof and method for treating animal excrements |
| CN103695331A (en) * | 2013-06-27 | 2014-04-02 | 深圳市新宝环保能源科技有限公司 | Bacillus coagulans and preparation method and application in biological deodorant thereof |
| CN105062932A (en) * | 2015-09-09 | 2015-11-18 | 肇庆大华农生物药品有限公司 | Microbial flora preparation applied to harmless dead poultry body disposal and preparation method and application thereof |
| CN106191178A (en) * | 2016-08-25 | 2016-12-07 | 江南大学 | Method for producing bacteriostatic active substance by using bacillus coagulans |
-
2016
- 2016-12-22 CN CN201611196824.6A patent/CN106635902B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101665773A (en) * | 2008-12-23 | 2010-03-10 | 中国农业科学院农业环境与可持续发展研究所 | Microbial bacterial agent, preparation method thereof and method for treating animal excrements |
| CN101638626A (en) * | 2009-09-10 | 2010-02-03 | 惠飞 | Bacillus subtilis and application thereof in biological deodorant |
| CN103695331A (en) * | 2013-06-27 | 2014-04-02 | 深圳市新宝环保能源科技有限公司 | Bacillus coagulans and preparation method and application in biological deodorant thereof |
| CN105062932A (en) * | 2015-09-09 | 2015-11-18 | 肇庆大华农生物药品有限公司 | Microbial flora preparation applied to harmless dead poultry body disposal and preparation method and application thereof |
| CN106191178A (en) * | 2016-08-25 | 2016-12-07 | 江南大学 | Method for producing bacteriostatic active substance by using bacillus coagulans |
Non-Patent Citations (6)
| Title |
|---|
| 姜锡瑞等: "《生物发酵产业技术》", 31 May 2016 * |
| 潘春梅: "《微生态制剂生产及应用》", 30 September 2014, 中国农业大学出版社 * |
| 赵必迁等: "凝结芽孢杆菌对蛋鸡生产性能的影响试验", 《科学种养》 * |
| 郭鹏等: "芽孢杆菌在畜禽废弃物污染治理中的研究进展", 《饲料研究》 * |
| 高书锋等: "凝结芽孢杆菌制剂对断奶仔猪临床应用效果的研究", 《饲料研究》 * |
| 黄遵锡等: "饲用凝结芽孢杆菌对蛋鸡生产性能及血液生化指标的影响", 《饲料工业》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107400637A (en) * | 2017-02-24 | 2017-11-28 | 上海道多生物科技有限公司 | A kind of bacillus coagulans and its microorganism formulation, preparation method and application |
| CN107400637B (en) * | 2017-02-24 | 2020-09-22 | 上海道多生物科技有限公司 | Bacillus coagulans and microbial preparation, preparation method and application thereof |
| CN107868763A (en) * | 2017-11-21 | 2018-04-03 | 青岛根源生物技术集团有限公司 | One plant is improved and attacks the bacillus coagulans E21 of the anti-oxidation stress ability of livestock and poultry animal and its application after poison |
| CN107868763B (en) * | 2017-11-21 | 2021-01-26 | 青岛根源生物技术集团有限公司 | Bacillus coagulans E21 for improving antioxidant stress capability of livestock and poultry after challenge and application thereof |
| CN108102967A (en) * | 2018-01-11 | 2018-06-01 | 天津生机集团股份有限公司 | One breeder source coagulating bacillus strain and its production spore method |
| CN110699298A (en) * | 2019-11-14 | 2020-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus coagulans P1-678 strain for producing urease inhibitor and application thereof |
| CN112048448A (en) * | 2020-07-27 | 2020-12-08 | 天津开发区坤禾生物技术有限公司 | Complex microbial inoculant for reducing ammonia odor of henhouse and preparation method and application thereof |
| CN111826323A (en) * | 2020-08-05 | 2020-10-27 | 福建洛东生物技术有限公司 | Bacillus subtilis, preparation and application thereof |
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