CN105754888B - Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation - Google Patents
Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation Download PDFInfo
- Publication number
- CN105754888B CN105754888B CN201510886295.1A CN201510886295A CN105754888B CN 105754888 B CN105754888 B CN 105754888B CN 201510886295 A CN201510886295 A CN 201510886295A CN 105754888 B CN105754888 B CN 105754888B
- Authority
- CN
- China
- Prior art keywords
- bacillus licheniformis
- bacterial agent
- microbial bacterial
- padding
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The present invention provides a kind of bacillus licheniformis (Bacillus licheniformis), the deposit number of the bacillus licheniformis is CGMCC No.11235.The present invention also provides a kind of microbial bacterial agent, which contains bacillus licheniformis as described above as effective component.The present invention also provides the application of bacillus licheniformis as described above and microbial bacterial agent as described above in fermentation bed cultivation.Through the above technical solutions, the present invention can more efficiently remove the stink of livestock culture.
Description
Technical field
The present invention relates to agricultural biological technical fields, and in particular, to a kind of bacillus licheniformis, a kind of microbial bacterial agent
With their applications in fermentation bed cultivation.
Background technique
In recent years, the development of China's livestock culture industry is swift and violent, and gradually tends to scale, intensive, but due to domestic livestock and poultry
The sewage disposal facility and fecaluria treatment measures of farm all lag far behind developed country from technology and scale, are discharged from farm
Waste water, exhaust gas, rubbish etc. serious pollution is all caused to the air, soil and water body of surrounding area, wherein especially with poultry
Farm's foul gas is the most significant to the sense organ and health effect of poultry and surrounding population.Livestock and poultry farm foul gas ingredient
Complexity, the method for conventional process foul gas are diligent cleanings, and multi-pass wind is rushed with water, and this method wastes a large amount of water resource,
Simultaneously increase humidity in farm, easily leads to poultry illness.
It is to solve the new method of livestock culture fecal pollution using fermentation bed cultivation technology.The principle of fermentation bed cultivation technology
It is to be mixed by a certain percentage with paddings such as sawdust, stalk, rice husks, microorganism is using fowl and animal excrement as nutrition using microbial bacterial agent
It is bred, the organic matter in fowl and animal excrement is made adequately to be decomposed and be converted, so that stink is made to disappear, and the life of microorganism
Long and breeding provides the nutriments such as mycoprotein to poultry simultaneously, thus formed one it is pollution-free, without discharge, without foul smell
Cultivating system.
Country's fermentation bed cultivation mode promotes and applies in the breeding productions such as pig at present, but existing fermentation bed strain
There is also excrement low efficiency is decomposed, the problems such as strain working life is short, easy to be dead, for main ammonia in farm's foul gas
The decomposition removal effect of gas and hydrogen sulfide gas is poor, and deodorizing effect is unobvious.
Summary of the invention
The defects of in order to overcome decomposition excrement low efficiency present in existing fermentation bed strain, the present invention provides one kind point
Solve the more efficient bacillus licheniformis of excrement.
The present inventor has separated a bacillus licheniformis, it is found that it efficiently can convert Asia for ammonium nitrogen
Nitrate nitrogen also has excellent product acid activity to reduce the generation of ammonia nitrogen, can maintain strain own growth environment
Acid condition results in the present invention.
The present invention provides a kind of bacillus licheniformis, the guarantor of the bacillus licheniformis (Bacillus licheniformis)
Hiding number is CGMCC No.11235.
The present invention also provides a kind of microbial bacterial agent, which contains thallus and culture medium, and the thallus contains
Having deposit number is the bacillus licheniformis of CGMCC No.11235.
The present invention also provides bacillus licheniformis as described above and microbial bacterial agent as described above to support in fermentation bed
Application in growing.
Through the above technical solutions, the present invention can maintain the low ph conditions of growth, extend the working life of strain,
While the ammonia nitrogen in fowl and animal excrement of more efficiently degrading, to mitigate farm's foul smell.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Biomaterial preservation
Bacillus licheniformis of the invention is the cow dung Chicken Manure Compost product that the present inventor acquires from Yanqing County of Beijing
The pure culture separated in sample, deposit number is CGMCC No.11235, the deposit date is on August 13rd, 2015, preservation
Unit is China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is located at BeiChen West Road, Chaoyang District, BeiJing City
No. 3 Institute of Microorganism, Academia Sinica of No. 1 institute, classification naming are bacillus licheniformis (Bacillus
licheniformis)。
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The present invention provides a kind of bacillus licheniformis, the bacillus licheniformis (Bacillus licheniformis)
Deposit number is CGMCC No.11235.
The present invention also provides a kind of microbial bacterial agent, which contains thallus and culture medium, and the thallus contains
Having deposit number is the bacillus licheniformis of CGMCC No.11235.
Wherein, the amount of the thallus contained in microbial bacterial agent can change in very large range, such as every gram of microbial bacteria
In agent, deposit number is that the viable count of the bacillus licheniformis of CGMCC No.11235 can be 108-1011Cfu, preferable case
Under, in every gram of microbial bacterial agent, deposit number is that the viable count of the bacillus licheniformis of CGMCC No.11235 can be 109-
1010cfu。
According to the present invention, the type of the culture medium can change in very large range, can be used in training to be various
The culture medium for supporting bacillus licheniformis, for example, can be beef-protein medium, nutrient broth medium, LB culture medium
Making above-mentioned culture medium etc. common culture medium can be commercially available or according to " microbiological culture media handbook " (Microbiology
Culture Media Manual) record be prepared.For example, culture medium can be beef-protein medium, contain
There is the sodium chloride of the beef extract of 1-5g/L, the peptone of 5-20g/L and 3-8g/L.
Wherein, above-mentioned various culture mediums are spare after being sterilized according to conventional sterilizing methods, such as in 115-125
DEG C and 1.5-2 standard atmospheric pressure under conditions of sterilize 10-30 minutes.
Wherein, the preparation method of the microbial bacterial agent may include: the ground for being CGMCC No.11235 by deposit number
Clothing bacillus, which is inoculated in culture medium, to be cultivated.Under preferable case, by cultivating the every gram of microbial bacteria made
In agent, deposit number is that the viable count of the bacillus licheniformis of CGMCC No.11235 can be 107-1011Cfu, more preferably
109-1010cfu.During culture, it can be obtained by conventional method, such as blood cell plate counting method or OD value observation
The concentration of viable bacteria.
Bacterium solution after culture can be used directly as microbial bacterial agent, and under preferable case, bacterium solution is by including sterile mistake
Filter, freeze-drying and etc. be further processed into more convenient storage the microbial bacterial agent of dosage form use.Wherein, the culture of strain
Condition is not particularly limited, and can be typical conditions in bacillus licheniformis incubation, for example, by using shaking table shake culture,
Cultivation temperature can be 28-37 DEG C, and incubation time can be 1-3 days.
The present invention also provides bacillus licheniformis as described above and microbial bacterial agent as described above to support in fermentation bed
It is applied to padding pre fermentation in growing.
According to the present invention, the application method of bacillus licheniformis and microbial bacterial agent in fermentation bed cultivation technology is without spy
Other requirement can be normally applied method for microbial inoculum in fermentation bed cultivation, for example, padding is mixed with microbial inoculum in proportion equal
It is even, adjust the padding heap fermentation that will be stirred evenly after moisture.
According to the present invention, fermentation bed contains padding and microbial bacterial agent, wherein the weight ratio of padding and microbial bacterial agent can
To change in very large range, under preferable case, the weight of microbial bacterial agent and padding can be than for 1:(900-1200).
According to the present invention, the hybrid mode of padding and microbial inoculum does not require particularly, padding can be accumulated in proportion,
Microbial inoculum is equably sprinkling upon on padding again, is sufficiently mixed until mixing completely.Padding adjusts the mode of moisture also without special
It is required that for example, can be during padding and microbial inoculum mix plus water, while being sufficiently mixed uniformly.
According to the present invention, the moisture content of padding does not require particularly, can be padding in normal fermentation bed cultural technique
Moisture content.Under preferable case, the water content of padding can be 40-50 weight %, the survey of the padding of water content within this range
Method for testing can grab padding for hand can be agglomerating, looses one's grip and dissipates, there is the feeling of water but the anhydrous exudation of webs on hand.
According to the present invention, padding type does not require particularly, can be padding kind common in fermentation bed cultivation technology
Class, under preferable case, padding can be for selected from one or more of sawdust, rice husk and wheat bran.
Wherein, as a kind of preferred embodiment of the invention, padding can be sawdust, the mixture of rice husk and wheat bran,
Relative to the sawdust of 100 parts by weight, the dosage of rice husk can be 60-70 parts by weight, and the dosage of wheat bran can be 5-10 parts by weight.
Microbial bacterial agent as described above can be first uniformly mixed with wheat bran, and the two mixture is sufficiently mixed with the mixture of sawdust and rice husk again
It is even, while water being added to adjust padding water content to 40-50 weight %.In the preferred embodiment, lichens gemma bar on the one hand can be allowed
Bacterium fast-growth during the fermentation, the fermenting bed padding on the other hand obtained for cultivate poultry can more effectively remove it is feeding
Grow stink.
According to the present invention, be uniformly mixed and adjust can be with heap fermentation after water content for padding, wherein the size of padding heap does not have
There is special requirement, as long as microbial bacterial agent normal fermentation can be made by meeting its internal temperature, under preferable case, padding heap
Volume can be in 10m3More than, the high 1.5m or more of padding heap.In order to increase the heat insulation effect in padding heap fermentation process, Ke Yi
Straw mattress or woven bag are covered on padding heap.
According to the present invention, above-mentioned fermentation bed cultivation may include fermentation bed to raise pig, fermentation bed poultry, fermentation bed duck culturing and hair
At least one of ferment bed sheep raising.
Present invention be described in more detail with reference to embodiments:
Embodiment 1
The present embodiment is for illustrating the culture of bacillus licheniformis of the invention and the preparation of microbial bacterial agent.
The bacillus licheniformis (Bacillus licheniformis) that deposit number is CGMCC No.11235 is inoculated with
To in LB culture medium (yeast extract of tryptone, 5g/L containing 10g/L and the NaCl of 10g/L), 30 DEG C and 200 turns/
Divide oscillation lower culture.Resulting bacterium solution is the microbial bacterial agent of the present embodiment, and in every gram of microbial bacterial agent, viable count is
109cfu。
Embodiment 2
The present embodiment is for illustrating the culture of bacillus licheniformis of the invention and the preparation of microbial bacterial agent.
The bacillus licheniformis (Bacillus licheniformis) that deposit number is CGMCC No.11235 is inoculated with
To in beef-protein medium (beef extract of peptone, 5g/L containing 20g/L and the NaCl of 5g/L), at 37 DEG C and 180
Rev/min lower culture of oscillation obtains bacterium solution.Resulting bacterium solution obtains the microorganism of the present embodiment through being sterile filtered after freeze-drying
Microbial inoculum, in every gram of microbial bacterial agent, viable count 1010cfu。
Embodiment 3
The present embodiment is for illustrating the culture of bacillus licheniformis of the invention and the preparation of microbial bacterial agent.
The bacillus licheniformis (Bacillus licheniformis) that deposit number is CGMCC No.11235 is inoculated with
To in nutrient broth medium (the beef extract powder of peptone, 3g/L containing 10g/L and the NaCl of 5g/L), 35 DEG C and 160 turns/
The lower culture of oscillation is divided to obtain bacterium solution.Resulting bacterium solution obtains the microbial bacteria of the present embodiment through being sterile filtered after freeze-drying
Agent, in every gram of microbial bacterial agent, viable count is 6 × 109cfu。
Comparative example 1
It is by the article number purchased from ATCC14580TMBacillus licheniformis (Bacillus
Licheniformis) it is inoculated into LB culture medium (yeast extract of tryptone, 5g/L containing 10g/L and the NaCl of 10g/L)
In, culture obtains bacterium solution under 35 DEG C and 120 revs/min oscillations.Resulting bacterium solution obtains this after freeze-drying through being sterile filtered
The microbial bacterial agent of embodiment, in every gram of microbial bacterial agent, viable count is 5 × 109cfu。
Comparative example 2
This comparative example is used for the microbial bacterial agent and preparation method thereof for illustrating to be different from the present invention.(1) in 100 parts by weight
Nutrient culture medium (content of peptone be the content of 0.8 weight %, NaCl be 0.4 weight %, the content of beef extract is
0.45 weight %, surplus be sterile water, pH 7.0) in inoculation 4 parts by weight bacillus licheniformis bacterial strain (ACCC 10146,
Purchased from Chinese agriculture Culture Collection), 37 DEG C of fermented and cultureds are sampled during the fermentation and pass through microscope
Direct counting method is observed, until the viable count of bacillus licheniformis is 0.8 × 1010A/gram；(2) in the battalion of 100 parts by weight
Support the bacterial strain (ACCC 10629, purchased from Chinese agriculture microbial bacteria that the bacillus subtilis of 2 parts by weight is inoculated in bouillon media
Kind collection), 37 DEG C of fermented and cultureds are sampled during the fermentation and are observed by ascites method,
Until the viable count of bacillus subtilis is 0.8 × 1010It is a/gram culture medium, wherein in the bouillon media, peptone
Content be 1 weight %, NaCl content be 0.25 weight %, the content of beef extract is 0.45 weight %, surplus is sterile water,
PH is 7.0.(3) in the bacterial strain (ACCC of the bacillus pumilus of 3 parts by weight of nutrient inoculation of medium of 100 parts by weight
10113, it is purchased from Chinese agriculture Culture Collection), 37 DEG C of fermented and cultureds are sampled during the fermentation and pass through
Ascites method is observed, until the viable count of bacillus pumilus is 0.8 × 1010A/gram, wherein the meat
In juice culture medium, the content of peptone be the content of 0.5 weight %, NaCl be 0.5 weight %, the content of beef extract is 0.3 weight
Measure %, surplus is sterile water, pH 7.0.(4) in the condensation gemma bar of 4 parts by weight of YPG inoculation of medium of 100 parts by weight
The bacterial strain (ACCC 10229 is purchased from Chinese agriculture Culture Collection) of bacterium, 37 DEG C of fermented and cultureds, during the fermentation
It is sampled and passes through ascites method and observed, until the viable count of bacillus coagulans is 0.8 × 1010A/
Gram, wherein in the YPG culture medium, the content of peptone is 0.8 weight %, the content of glucose is 0.3 weight %, yeast
The content of cream is 0.8 weight %, surplus is sterile water, and the pH of the culture medium is 7.2.(5) in the brewer's wort culture of 100 parts by weight
(it is micro- to be purchased from Chinese agriculture to the Wine brewing yeast strain of 3 parts by weight of inoculation by ACCC 20237 in base (Shanghai crystalline substance pure reagent Co., Ltd)
Biological inoculum collection), 30 DEG C of fermented and cultureds are sampled during the fermentation and are carried out by ascites method
Observation, until the viable count of saccharomyces cerevisiae is 0.8 × 1010It is a/gram culture medium.(6) No.1 is synthesized in the Gao Shi of 100 parts by weight
Bacterial strain (the ACCC 40126, purchased from Chinese agriculture Microbiological Culture Collection of the Streptomyces jingyangensis of 4 parts by weight of inoculation of medium
The heart), 30 DEG C of fermentations are sampled and are observed by ascites method, during the fermentation until Jingyang strepto-
The viable count of bacterium is 0.8 × 1010A/gram, wherein in the Gause I culture medium, on the basis of the total weight of the culture medium,
The content of soluble starch is 2 weight %, KNO3Content be 0.2 weight %, K2HPO4Content be 0.7 weight %, NaCl
Content is 0.03 weight %, surplus is sterile water, and the pH of the culture medium is 7.2.(7) by lichens obtained in step (1) to (6)
Bacillus, bacillus subtilis, bacillus pumilus, bacillus coagulans, saccharomyces cerevisiae and Streptomyces jingyangensis are proportionally
It is mixed, in the microbial bacterial agent made, on the basis of total viable count of obtained microbial bacterial agent, bacillus licheniformis
Viable count be 15 weight % of total viable count, the viable count of bacillus subtilis is the 15% of total viable count, short and small gemma bar
The work that the viable count of bacterium is the 20% of total viable count, the viable count of bacillus coagulans is the 20% of total viable count, saccharomyces cerevisiae
Bacterium number is the 15% of total viable count, the viable count of Streptomyces jingyangensis is the 15% of total viable count.
Embodiment 4
The present embodiment is for illustrating microbial bacterial agent of the present invention for the pre-fermented side of padding in fermentation bed cultivation
Method.The present embodiment uses microbial bacterial agent same as Example 3.
The pine wood sawdust of 100 parts by weight and the rice husk of 66 parts by weight are taken, 20 parts by weight water are spilled into, is uniformly mixed and heap is good,
After mixing by the wheat bran of 6 parts by weight and the microbial inoculum of 0.17 parts by weight, it is uniformly sprinkling upon on the sawdust and rice husk accumulated, by institute
There is raw material to be fully mixed to substantially uniformity, while adjusting moisture to padding water content is 45%, mixed padding presses every heap 10m3,
High 1.5m accumulates, and straw mattress heat-preservation fermentation is covered on padding, as 55 DEG C of padding temperature >, can be used after spreading out.
Embodiment 5
The present embodiment is for illustrating microbial bacterial agent of the present invention for the pre-fermented side of padding in fermentation bed cultivation
Method.The present embodiment uses microbial bacterial agent same as Example 2.
The pine wood sawdust of 100 parts by weight and the rice husk of 60 parts by weight are taken, 20 parts by weight water are spilled into, is uniformly mixed and heap is good,
After mixing by the wheat bran of 10 parts by weight and the microbial inoculum of 0.14 parts by weight, it is uniformly sprinkling upon on the sawdust and rice husk accumulated, it will
All raw materials are fully mixed to substantially uniformity, while adjusting moisture to padding water content is 40%, and mixed padding presses every heap
10m3, high 1.5m accumulation covers straw mattress heat-preservation fermentation on padding, i.e. usable after spreading out as 55 DEG C of padding temperature >.
Embodiment 6
The present embodiment is for illustrating microbial bacterial agent of the present invention for the pre-fermented side of padding in fermentation bed cultivation
Method.The present embodiment uses microbial bacterial agent same as Example 1.
The pine wood sawdust of 100 parts by weight and the rice husk of 70 parts by weight are taken, 20 parts by weight water are spilled into, is uniformly mixed and heap is good,
After mixing by the microbial inoculum of 0.21 parts by weight, it is uniformly sprinkling upon on the sawdust and rice husk accumulated, all raw materials is sufficiently mixed
To substantially uniformity, while adjusting moisture to padding water content is 50%, and mixed padding presses every heap 10m3, high 1.5m accumulation, pad
Straw mattress heat-preservation fermentation is covered on material, as 55 DEG C of padding temperature >, can be used after spreading out.
Comparative example 3
Using the padding pre fermentation method in embodiment 4, the difference is that using microbial bacteria identical with comparative example 1
Agent.
Comparative example 4
This comparative example is used for the pad material fermentation method for illustrating to be different from the present invention, and uses microorganism identical with comparative example 2
Microbial inoculum.
(1) prepare padding according to following weight ratio: the dosage of rice straw is 20 weight %, and the dosage of rice husk is 30 weights
% is measured, the dosage of sawdust is 40 weight %, and the dosage of rice bran is 10 weight %.(2) using microbial bacterial agent made from comparative example 2
The padding is inoculated with, relative to the base-material of 100 parts by weight, the inoculum concentration of microbial bacterial agent is 3 parts by weight.(3) it docks
Padding after kind ferments, and fermentation temperature is 50 DEG C, and fermentation time is 2 days.(4) padding after fermentation is carried out in pig house
Windrow, the height of windrow are 55 centimeters, and temperature maintains 35-60 DEG C in windrow, which is 150 days using the time, pig house
Temperature maintain 15-35 DEG C.
Testing example 1
This testing example measures the ability of the degradation of ammonia nitrogen of the microbial bacterial agent of embodiment 1-3 and comparative example 1-2.
Prepare sterile beef-protein medium respectively, 100mL culture medium be packed into the conical flask of 250mL, respectively plus
The microbial bacterial agent 0.05g for entering embodiment 1-3 and comparative example 1 is placed in 37 DEG C of cultures for 24 hours.
Prepare ammonia oxidation bacteria culture medium ((NH4)2SO42g/L, NaCl 0.3g/L, FeSO4·7H2O 0.03g/L,
MgSO4·7H2O 0.03g/L, K2HPO41g/L, NaHCO31.6g/L, agar powder 18g/L use 1N sodium hydroxide solution tune
For whole pH to 7.2), every bottle of 100mL accesses the above-mentioned each bacterium solution of 1mL.After culture 4 days, the concentration of ammonia nitrogen in culture medium is detected, as a result
It is listed in table 1.
Table 1
According to the data of table 1 as it can be seen that deposit number of the invention is the bacillus licheniformis of CGMCC No.11235
(Bacillus licheniformis) and its microbial inoculum have the energy of degradation of ammonia nitrogen outstanding relative to other microorganisms and microbial inoculum
Power.
Testing example 2
This testing example measures the acid producing ability of the microbial bacterial agent of embodiment 1-3 and comparative example 1-2.
Prepare sterile beef-protein medium respectively, 100mL culture medium be packed into the conical flask of 250mL, respectively plus
The microbial bacterial agent 0.05g for entering embodiment 1-3 and comparative example 1 is placed in 37 DEG C of cultures for 24 hours.
Prepare acid-producing bacteria culture medium (glucose 6g/L, yeast extract 1g/L, peptone 1g/L, MgSO40.05g/L, CaCO3
1g/L, bromocresol purple 0.04g/L), acid-producing bacteria culture medium 8mL is placed in teat glass (18mm*180mm), sterilizing is good spare.
The above-mentioned each bacterium solution of 1mL is accessed, 37 DEG C, 170r/min shake culture are placed in.It cultivates and sees whether that bromocresol purple is made to change colour for 24 hours afterwards,
And the pH value in each sample tube is measured, as a result it is listed in table 2.
Table 2
| Microbial inoculum | Whether change colour | pH |
| Embodiment 1 | It is | 5.12 |
| Embodiment 2 | It is | 5.01 |
| Embodiment 3 | It is | 4.93 |
| Comparative example 1 | It is no | 7.09 |
| Comparative example 2 | It is no | 6.83 |
According to the data of table 2 as it can be seen that deposit number of the invention is the bacillus licheniformis of CGMCC No.11235
(Bacillus licheniformis) and its microbial inoculum have stronger acid producing ability relative to other microorganisms and microbial inoculum, can be with
Acidic environment needed for maintaining microorganism growth.
Testing example 3
This testing example measures the performance of fermentation bed in embodiment 4-6 and comparative example 3-4 padding pre fermentation method.Test
PH value, temperature and the total number of bacteria of padding, are as a result listed in table 3 respectively, in table 4 and table 5.The test method of pH value are as follows: take quite
In the air-dry sample of the fresh sample of 2g, add 50mL distilled water, vibrated in shaking table and mix 20min, stands 2h, measure the pH value of supernatant；
The measuring method of temperature is thermometer to be inserted perpendicularly into from ramped surfaces to material heap center, in depth apart from 1 meter of ground eminence in material heap
Degree is that long handle metallic thermometer measuring temperature is used at 30cm, 60cm and 90cm, is averaged；The measuring method of total number of bacteria is dilute
Release spread plate method.
Table 3
According to the data of table 3 as it can be seen that microbial inoculum of the invention can buffer the pH value for adjusting fermentation padding, it is made to maintain phase
To neutral condition, this is conducive to the flourish of probiotics, is conducive to quickly disappearing for feces of livestock and poultry when using in poultry house
Solution.
Table 4
It can be seen that microbial inoculum of the invention according to the data of table 4 to be added in fermenting bed padding, fermentation calefaction speed is fast,
Fermenting third day can be into the megathermal period, this illustrates that the bacteria fermentation speed is fast, high-efficient.From embodiment 4-5 and embodiment 6
Data comparison can be seen that preferred fermenting bed padding be sawdust, rice husk and wheat bran be used in conjunction with preparation fermentation bed in the case where,
The fermenting speed of microbial inoculum is faster.
Table 5
According to the data of table 5 as it can be seen that compared with comparative example 3-4, it can be cultivated in microbial bacterial agent used in embodiment 4-6
Total number of bacteria is higher, and the deposit number wherein contained in microbial inoculum is the bacillus licheniformis of CGMCC No.11235
The predominant number of (Bacillus licheniformis), and the growth rate of the bacillus licheniformis in microbial inoculum is quickly,
Miscellaneous bacteria in padding gradually tails off.This is because most der Pilz is killed at 55 DEG C of >, in addition there are many bacteriums miscellaneous
Bacterium also non-refractory, but the bacillus licheniformis in microbial inoculum under the high temperature conditions can suspend mode become when after the megathermal period as padding
In main bacteria seed.Can be seen that preferred fermenting bed padding from the data comparison of embodiment 4-5 and embodiment 6 is sawdust, rice
In the case that shell and wheat bran are used in conjunction with preparation fermentation bed, the microbial inoculum bacterium in padding is more, and growth rate is also faster.
Testing example 4
The padding pre fermentation method that this testing example measures embodiment 4-6 and comparative example 3-4 is used for fermentation bed cultivation skill
The effect of art.
Select identical age in days, Du × length × Dasanyuan of weight close (about 10kg) hybridizes porkling 100, according to weight and
Principle similar in sex ratio is equally divided into 5 groups, and microbial bacterial agent identical with embodiment 4-6 and comparative example 3-4 is respectively adopted
Fermentation bed is prepared with padding pre fermentation method.
Above-mentioned 5 groups of porklings feed 70 days, the growth performance of pig house environment situation and pig are tested and count, as a result such as 6 institute of table
Show.
Table 6
| Group | Colony house situation | Pig growing state |
| Embodiment 4 | Without obvious stink | Hair color is bright, and pig body is clean |
| Embodiment 5 | Without obvious stink | Hair color is bright, and pig body is clean |
| Embodiment 6 | Without obvious stink | Hair color is bright, and pig body is clean |
| Comparative example 3 | Stench | Dull, pig body are dirtier |
| Comparative example 4 | There is stink | Dull, pig body are dirtier |
The colony house environment that can be seen that embodiment 4-6 according to the data of table 6 is obviously better than comparative example 3-4, illustrates using this
The microbial bacterial agent of invention can efficiently eliminate cultivation stench, improve the environment of cultivation colony house, reduce stink to environmental emission.
Table 7
| Group | First weight/kg | Last weight/kg | Daily gain/kg | Feedstuff-meat ratio |
| Embodiment 4 | 10.56±1.45 | 52.47±5.09 | 0.60±0.03 | 2.34 |
| Embodiment 5 | 10.37±1.53 | 51.67±4.15 | 0.59±0.04 | 2.38 |
| Embodiment 6 | 10.41±1.48 | 51.01±4.15 | 0.58±0.04 | 2.41 |
| Comparative example 3 | 10.44±1.22 | 44.57±4.23 | 0.49±0.04 | 2.88 |
| Comparative example 4 | 10.48±1.22 | 47.70±4.23 | 0.53±0.04 | 2.64 |
It can be seen that the various aspects growths such as daily gain and the feedstuff-meat ratio that pig is cultivated in embodiment 4-6 according to the data of table 7
The cultivation pig that can be substantially better than in comparative example 3-4, this is because disappearing after microbial bacterial agent of the invention is applied to fermentation bed cultivation
In addition to cultivating colony house foul smell, so that the growing environment for cultivating pig is greatly improved, promote the growth of cultivation pig, improve the effect of cultivation
Benefit.Can be seen that preferred fermenting bed padding from the data comparison of embodiment 4-5 and embodiment 6 is that sawdust, rice husk and wheat bran are total
In the case where using preparation fermentation bed, the growth performance for cultivating pig is more excellent.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of bacillus licheniformis, it is characterised in that: the guarantor of the bacillus licheniformis (Bacillus licheniformis)
Hiding number is CGMCC No.11235.
2. a kind of microbial bacterial agent, which contains thallus and culture medium, it is characterised in that: the thallus is preservation volume
Number be CGMCC No.11235 bacillus licheniformis.
3. microbial bacterial agent according to claim 2, it is characterised in that: in every gram of microbial bacterial agent, deposit number
Viable count for the bacillus licheniformis of CGMCC No.11235 is 109-1010cfu。
4. microbial bacterial agent according to claim 2 or 3, it is characterised in that: the culture medium is beef extract-peptone training
Support at least one of base, nutrient broth medium and LB culture medium.
5. microbial bacterial agent described in any one of bacillus licheniformis described in claim 1 and claim 2-4 is being sent out
Application in the cultivation of ferment bed.
6. 5 application according to claim, it is characterised in that: the fermentation bed contains padding and the microbial bacterial agent,
Wherein, the weight ratio of microbial bacterial agent and padding is 1:(900-1200).
7. application according to claim 5 or 6, it is characterised in that: the moisture content of the padding is 40-50 weight %.
8. application according to claim 5 or 6, it is characterised in that: the padding is in sawdust, rice husk and wheat bran
It is one or more of.
9. application according to claim 8, it is characterised in that: relative to the sawdust of 100 parts by weight, the dosage of rice husk is
60-70 parts by weight, the dosage of wheat bran are 5-10 parts by weight.
10. application according to claim 5, it is characterised in that: the fermentation bed cultivation includes fermentation bed to raise pig, fermentation bed
At least one of poultry, fermentation bed duck culturing and fermentation bed sheep raising.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510886295.1A CN105754888B (en) | 2015-12-04 | 2015-12-04 | Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510886295.1A CN105754888B (en) | 2015-12-04 | 2015-12-04 | Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105754888A CN105754888A (en) | 2016-07-13 |
| CN105754888B true CN105754888B (en) | 2019-01-25 |
Family
ID=56342109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510886295.1A Active CN105754888B (en) | 2015-12-04 | 2015-12-04 | Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105754888B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330078B (en) * | 2017-08-14 | 2021-10-08 | 北京瓜尔润科技股份有限公司 | Bacillus licheniformis for improving crude protein yield in guar meal fermentation and application thereof |
| CN107502572B (en) * | 2017-09-08 | 2020-08-21 | 中国农业科学院农业环境与可持续发展研究所 | Application of bacillus licheniformis in straw degradation, microbial agent containing bacillus licheniformis and application of bacillus licheniformis |
| CN110699299B (en) * | 2019-11-14 | 2023-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof |
| CN110964639A (en) * | 2019-12-09 | 2020-04-07 | 湖南金泥生物科技有限公司 | Strain screening method applied to pig raising fermentation bed and application |
| CN114806936B (en) * | 2022-04-14 | 2023-06-09 | 上海市农业科学院 | Bacillus licheniformis with antibacterial and simple substance selenium synthesizing capabilities and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286376A (en) * | 2011-06-21 | 2011-12-21 | 湖南省微生物研究所 | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof |
| CN104126515A (en) * | 2014-07-21 | 2014-11-05 | 上海金泥生物科技有限公司 | Livestock and poultry biodegradable pad |
-
2015
- 2015-12-04 CN CN201510886295.1A patent/CN105754888B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286376A (en) * | 2011-06-21 | 2011-12-21 | 湖南省微生物研究所 | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof |
| CN104126515A (en) * | 2014-07-21 | 2014-11-05 | 上海金泥生物科技有限公司 | Livestock and poultry biodegradable pad |
Non-Patent Citations (2)
| Title |
|---|
| 发酵床中耐高温地衣芽孢的分离鉴定及产酶分析;尹红梅等;《家畜生态学报》;20121115;第33卷(第6期);97-102 * |
| 尹红梅等.发酵床中耐高温地衣芽孢的分离鉴定及产酶分析.《家畜生态学报》.2012,第33卷(第6期),97-102. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105754888A (en) | 2016-07-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104293694B (en) | A kind of preparation method of sludge aerobic compost composite bacteria agent | |
| US8778048B2 (en) | Biochemical humic acid product prepared from kitchen waste and the method of preparing the same | |
| CN105754888B (en) | Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation | |
| CN102286376B (en) | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof | |
| CN109022327B (en) | Preparation method of microbial mixed inoculant and application of microbial mixed inoculant in high-temperature composting | |
| CN102491802A (en) | Tea seed cake biological organic fertilizer and production method thereof | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN108048344B (en) | Two plants of deodorization bacterial strains and its application in preparation composite biological deodorant | |
| CN104193516A (en) | Environment-friendly fertilizer for strawberries and preparation method thereof | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN109762765B (en) | Decomposed solid fermentation microbial inoculum and application thereof in agricultural wastes | |
| CN105112311B (en) | Pichia pastoris YPD-YL2 and its application in biological deodorizing | |
| CN106635902A (en) | Bacillus coagulans and application thereof | |
| CN107177533A (en) | A kind of Thermophilic Bacteria compounding microbial inoculum and preparation method and application | |
| CN107177521A (en) | A kind of composite bacteria agent for the organic fertilizer that ferments and its preparation method and application | |
| CN106305487A (en) | Biological active padding material of fermentation bed for poultry, preparation method and application | |
| CN105255777A (en) | Method for producing bio-organic fertilizer through pig manure and mushroom residues | |
| CN109279748A (en) | A kind of pig farm microbial deodorant and preparation method | |
| CN110438031A (en) | A kind of microbial deoderizer and preparation method thereof | |
| CN105255761B (en) | A kind of deodorization microbial inoculum and its application method for organic waste treatment | |
| CN102432146B (en) | Complex microbial inoculum for efficiently converting poultry culture excrement and preparation method thereof | |
| CN108251331A (en) | Water balsamine Bacillus alcaligenes and microbial bacterial agent and their applications in animal waste recycling | |
| CN105543149A (en) | Novel bacillus megaterium and application thereof | |
| CN109957527A (en) | A kind of direct decomposing and fermenting crops straws produce special microorganism composite bacterial agent preparation and its application method of biogas | |
| CN110066748A (en) | A kind of complex microorganism and microbial inoculum and its application comprising the complex microorganism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |