CN105754888B - Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation - Google Patents

Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation Download PDF

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CN105754888B
CN105754888B CN201510886295.1A CN201510886295A CN105754888B CN 105754888 B CN105754888 B CN 105754888B CN 201510886295 A CN201510886295 A CN 201510886295A CN 105754888 B CN105754888 B CN 105754888B
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bacillus licheniformis
bacterial agent
microbial bacterial
padding
weight
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CN105754888A (en
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张丽
耿兵
朱昌雄
田云龙
叶婧
郭萍
李红娜
刘雪
李峰
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Institute of Environment and Sustainable Development in Agriculturem of CAAS
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Institute of Environment and Sustainable Development in Agriculturem of CAAS
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Abstract

The present invention provides a kind of bacillus licheniformis (Bacillus licheniformis), the deposit number of the bacillus licheniformis is CGMCC No.11235.The present invention also provides a kind of microbial bacterial agent, which contains bacillus licheniformis as described above as effective component.The present invention also provides the application of bacillus licheniformis as described above and microbial bacterial agent as described above in fermentation bed cultivation.Through the above technical solutions, the present invention can more efficiently remove the stink of livestock culture.

Description

Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation
Technical field
The present invention relates to agricultural biological technical fields, and in particular, to a kind of bacillus licheniformis, a kind of microbial bacterial agent With their applications in fermentation bed cultivation.
Background technique
In recent years, the development of China's livestock culture industry is swift and violent, and gradually tends to scale, intensive, but due to domestic livestock and poultry The sewage disposal facility and fecaluria treatment measures of farm all lag far behind developed country from technology and scale, are discharged from farm Waste water, exhaust gas, rubbish etc. serious pollution is all caused to the air, soil and water body of surrounding area, wherein especially with poultry Farm's foul gas is the most significant to the sense organ and health effect of poultry and surrounding population.Livestock and poultry farm foul gas ingredient Complexity, the method for conventional process foul gas are diligent cleanings, and multi-pass wind is rushed with water, and this method wastes a large amount of water resource, Simultaneously increase humidity in farm, easily leads to poultry illness.
It is to solve the new method of livestock culture fecal pollution using fermentation bed cultivation technology.The principle of fermentation bed cultivation technology It is to be mixed by a certain percentage with paddings such as sawdust, stalk, rice husks, microorganism is using fowl and animal excrement as nutrition using microbial bacterial agent It is bred, the organic matter in fowl and animal excrement is made adequately to be decomposed and be converted, so that stink is made to disappear, and the life of microorganism Long and breeding provides the nutriments such as mycoprotein to poultry simultaneously, thus formed one it is pollution-free, without discharge, without foul smell Cultivating system.
Country's fermentation bed cultivation mode promotes and applies in the breeding productions such as pig at present, but existing fermentation bed strain There is also excrement low efficiency is decomposed, the problems such as strain working life is short, easy to be dead, for main ammonia in farm's foul gas The decomposition removal effect of gas and hydrogen sulfide gas is poor, and deodorizing effect is unobvious.
Summary of the invention
The defects of in order to overcome decomposition excrement low efficiency present in existing fermentation bed strain, the present invention provides one kind point Solve the more efficient bacillus licheniformis of excrement.
The present inventor has separated a bacillus licheniformis, it is found that it efficiently can convert Asia for ammonium nitrogen Nitrate nitrogen also has excellent product acid activity to reduce the generation of ammonia nitrogen, can maintain strain own growth environment Acid condition results in the present invention.
The present invention provides a kind of bacillus licheniformis, the guarantor of the bacillus licheniformis (Bacillus licheniformis) Hiding number is CGMCC No.11235.
The present invention also provides a kind of microbial bacterial agent, which contains thallus and culture medium, and the thallus contains Having deposit number is the bacillus licheniformis of CGMCC No.11235.
The present invention also provides bacillus licheniformis as described above and microbial bacterial agent as described above to support in fermentation bed Application in growing.
Through the above technical solutions, the present invention can maintain the low ph conditions of growth, extend the working life of strain, While the ammonia nitrogen in fowl and animal excrement of more efficiently degrading, to mitigate farm's foul smell.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Biomaterial preservation
Bacillus licheniformis of the invention is the cow dung Chicken Manure Compost product that the present inventor acquires from Yanqing County of Beijing The pure culture separated in sample, deposit number is CGMCC No.11235, the deposit date is on August 13rd, 2015, preservation Unit is China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is located at BeiChen West Road, Chaoyang District, BeiJing City No. 3 Institute of Microorganism, Academia Sinica of No. 1 institute, classification naming are bacillus licheniformis (Bacillus licheniformis)。
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The present invention provides a kind of bacillus licheniformis, the bacillus licheniformis (Bacillus licheniformis) Deposit number is CGMCC No.11235.
The present invention also provides a kind of microbial bacterial agent, which contains thallus and culture medium, and the thallus contains Having deposit number is the bacillus licheniformis of CGMCC No.11235.
Wherein, the amount of the thallus contained in microbial bacterial agent can change in very large range, such as every gram of microbial bacteria In agent, deposit number is that the viable count of the bacillus licheniformis of CGMCC No.11235 can be 108-1011Cfu, preferable case Under, in every gram of microbial bacterial agent, deposit number is that the viable count of the bacillus licheniformis of CGMCC No.11235 can be 109- 1010cfu。
According to the present invention, the type of the culture medium can change in very large range, can be used in training to be various The culture medium for supporting bacillus licheniformis, for example, can be beef-protein medium, nutrient broth medium, LB culture medium Making above-mentioned culture medium etc. common culture medium can be commercially available or according to " microbiological culture media handbook " (Microbiology Culture Media Manual) record be prepared.For example, culture medium can be beef-protein medium, contain There is the sodium chloride of the beef extract of 1-5g/L, the peptone of 5-20g/L and 3-8g/L.
Wherein, above-mentioned various culture mediums are spare after being sterilized according to conventional sterilizing methods, such as in 115-125 DEG C and 1.5-2 standard atmospheric pressure under conditions of sterilize 10-30 minutes.
Wherein, the preparation method of the microbial bacterial agent may include: the ground for being CGMCC No.11235 by deposit number Clothing bacillus, which is inoculated in culture medium, to be cultivated.Under preferable case, by cultivating the every gram of microbial bacteria made In agent, deposit number is that the viable count of the bacillus licheniformis of CGMCC No.11235 can be 107-1011Cfu, more preferably 109-1010cfu.During culture, it can be obtained by conventional method, such as blood cell plate counting method or OD value observation The concentration of viable bacteria.
Bacterium solution after culture can be used directly as microbial bacterial agent, and under preferable case, bacterium solution is by including sterile mistake Filter, freeze-drying and etc. be further processed into more convenient storage the microbial bacterial agent of dosage form use.Wherein, the culture of strain Condition is not particularly limited, and can be typical conditions in bacillus licheniformis incubation, for example, by using shaking table shake culture, Cultivation temperature can be 28-37 DEG C, and incubation time can be 1-3 days.
The present invention also provides bacillus licheniformis as described above and microbial bacterial agent as described above to support in fermentation bed It is applied to padding pre fermentation in growing.
According to the present invention, the application method of bacillus licheniformis and microbial bacterial agent in fermentation bed cultivation technology is without spy Other requirement can be normally applied method for microbial inoculum in fermentation bed cultivation, for example, padding is mixed with microbial inoculum in proportion equal It is even, adjust the padding heap fermentation that will be stirred evenly after moisture.
According to the present invention, fermentation bed contains padding and microbial bacterial agent, wherein the weight ratio of padding and microbial bacterial agent can To change in very large range, under preferable case, the weight of microbial bacterial agent and padding can be than for 1:(900-1200).
According to the present invention, the hybrid mode of padding and microbial inoculum does not require particularly, padding can be accumulated in proportion, Microbial inoculum is equably sprinkling upon on padding again, is sufficiently mixed until mixing completely.Padding adjusts the mode of moisture also without special It is required that for example, can be during padding and microbial inoculum mix plus water, while being sufficiently mixed uniformly.
According to the present invention, the moisture content of padding does not require particularly, can be padding in normal fermentation bed cultural technique Moisture content.Under preferable case, the water content of padding can be 40-50 weight %, the survey of the padding of water content within this range Method for testing can grab padding for hand can be agglomerating, looses one's grip and dissipates, there is the feeling of water but the anhydrous exudation of webs on hand.
According to the present invention, padding type does not require particularly, can be padding kind common in fermentation bed cultivation technology Class, under preferable case, padding can be for selected from one or more of sawdust, rice husk and wheat bran.
Wherein, as a kind of preferred embodiment of the invention, padding can be sawdust, the mixture of rice husk and wheat bran, Relative to the sawdust of 100 parts by weight, the dosage of rice husk can be 60-70 parts by weight, and the dosage of wheat bran can be 5-10 parts by weight. Microbial bacterial agent as described above can be first uniformly mixed with wheat bran, and the two mixture is sufficiently mixed with the mixture of sawdust and rice husk again It is even, while water being added to adjust padding water content to 40-50 weight %.In the preferred embodiment, lichens gemma bar on the one hand can be allowed Bacterium fast-growth during the fermentation, the fermenting bed padding on the other hand obtained for cultivate poultry can more effectively remove it is feeding Grow stink.
According to the present invention, be uniformly mixed and adjust can be with heap fermentation after water content for padding, wherein the size of padding heap does not have There is special requirement, as long as microbial bacterial agent normal fermentation can be made by meeting its internal temperature, under preferable case, padding heap Volume can be in 10m3More than, the high 1.5m or more of padding heap.In order to increase the heat insulation effect in padding heap fermentation process, Ke Yi Straw mattress or woven bag are covered on padding heap.
According to the present invention, above-mentioned fermentation bed cultivation may include fermentation bed to raise pig, fermentation bed poultry, fermentation bed duck culturing and hair At least one of ferment bed sheep raising.
Present invention be described in more detail with reference to embodiments:
Embodiment 1
The present embodiment is for illustrating the culture of bacillus licheniformis of the invention and the preparation of microbial bacterial agent.
The bacillus licheniformis (Bacillus licheniformis) that deposit number is CGMCC No.11235 is inoculated with To in LB culture medium (yeast extract of tryptone, 5g/L containing 10g/L and the NaCl of 10g/L), 30 DEG C and 200 turns/ Divide oscillation lower culture.Resulting bacterium solution is the microbial bacterial agent of the present embodiment, and in every gram of microbial bacterial agent, viable count is 109cfu。
Embodiment 2
The present embodiment is for illustrating the culture of bacillus licheniformis of the invention and the preparation of microbial bacterial agent.
The bacillus licheniformis (Bacillus licheniformis) that deposit number is CGMCC No.11235 is inoculated with To in beef-protein medium (beef extract of peptone, 5g/L containing 20g/L and the NaCl of 5g/L), at 37 DEG C and 180 Rev/min lower culture of oscillation obtains bacterium solution.Resulting bacterium solution obtains the microorganism of the present embodiment through being sterile filtered after freeze-drying Microbial inoculum, in every gram of microbial bacterial agent, viable count 1010cfu。
Embodiment 3
The present embodiment is for illustrating the culture of bacillus licheniformis of the invention and the preparation of microbial bacterial agent.
The bacillus licheniformis (Bacillus licheniformis) that deposit number is CGMCC No.11235 is inoculated with To in nutrient broth medium (the beef extract powder of peptone, 3g/L containing 10g/L and the NaCl of 5g/L), 35 DEG C and 160 turns/ The lower culture of oscillation is divided to obtain bacterium solution.Resulting bacterium solution obtains the microbial bacteria of the present embodiment through being sterile filtered after freeze-drying Agent, in every gram of microbial bacterial agent, viable count is 6 × 109cfu。
Comparative example 1
It is by the article number purchased from ATCC14580TMBacillus licheniformis (Bacillus Licheniformis) it is inoculated into LB culture medium (yeast extract of tryptone, 5g/L containing 10g/L and the NaCl of 10g/L) In, culture obtains bacterium solution under 35 DEG C and 120 revs/min oscillations.Resulting bacterium solution obtains this after freeze-drying through being sterile filtered The microbial bacterial agent of embodiment, in every gram of microbial bacterial agent, viable count is 5 × 109cfu。
Comparative example 2
This comparative example is used for the microbial bacterial agent and preparation method thereof for illustrating to be different from the present invention.(1) in 100 parts by weight Nutrient culture medium (content of peptone be the content of 0.8 weight %, NaCl be 0.4 weight %, the content of beef extract is 0.45 weight %, surplus be sterile water, pH 7.0) in inoculation 4 parts by weight bacillus licheniformis bacterial strain (ACCC 10146, Purchased from Chinese agriculture Culture Collection), 37 DEG C of fermented and cultureds are sampled during the fermentation and pass through microscope Direct counting method is observed, until the viable count of bacillus licheniformis is 0.8 × 1010A/gram;(2) in the battalion of 100 parts by weight Support the bacterial strain (ACCC 10629, purchased from Chinese agriculture microbial bacteria that the bacillus subtilis of 2 parts by weight is inoculated in bouillon media Kind collection), 37 DEG C of fermented and cultureds are sampled during the fermentation and are observed by ascites method, Until the viable count of bacillus subtilis is 0.8 × 1010It is a/gram culture medium, wherein in the bouillon media, peptone Content be 1 weight %, NaCl content be 0.25 weight %, the content of beef extract is 0.45 weight %, surplus is sterile water, PH is 7.0.(3) in the bacterial strain (ACCC of the bacillus pumilus of 3 parts by weight of nutrient inoculation of medium of 100 parts by weight 10113, it is purchased from Chinese agriculture Culture Collection), 37 DEG C of fermented and cultureds are sampled during the fermentation and pass through Ascites method is observed, until the viable count of bacillus pumilus is 0.8 × 1010A/gram, wherein the meat In juice culture medium, the content of peptone be the content of 0.5 weight %, NaCl be 0.5 weight %, the content of beef extract is 0.3 weight Measure %, surplus is sterile water, pH 7.0.(4) in the condensation gemma bar of 4 parts by weight of YPG inoculation of medium of 100 parts by weight The bacterial strain (ACCC 10229 is purchased from Chinese agriculture Culture Collection) of bacterium, 37 DEG C of fermented and cultureds, during the fermentation It is sampled and passes through ascites method and observed, until the viable count of bacillus coagulans is 0.8 × 1010A/ Gram, wherein in the YPG culture medium, the content of peptone is 0.8 weight %, the content of glucose is 0.3 weight %, yeast The content of cream is 0.8 weight %, surplus is sterile water, and the pH of the culture medium is 7.2.(5) in the brewer's wort culture of 100 parts by weight (it is micro- to be purchased from Chinese agriculture to the Wine brewing yeast strain of 3 parts by weight of inoculation by ACCC 20237 in base (Shanghai crystalline substance pure reagent Co., Ltd) Biological inoculum collection), 30 DEG C of fermented and cultureds are sampled during the fermentation and are carried out by ascites method Observation, until the viable count of saccharomyces cerevisiae is 0.8 × 1010It is a/gram culture medium.(6) No.1 is synthesized in the Gao Shi of 100 parts by weight Bacterial strain (the ACCC 40126, purchased from Chinese agriculture Microbiological Culture Collection of the Streptomyces jingyangensis of 4 parts by weight of inoculation of medium The heart), 30 DEG C of fermentations are sampled and are observed by ascites method, during the fermentation until Jingyang strepto- The viable count of bacterium is 0.8 × 1010A/gram, wherein in the Gause I culture medium, on the basis of the total weight of the culture medium, The content of soluble starch is 2 weight %, KNO3Content be 0.2 weight %, K2HPO4Content be 0.7 weight %, NaCl Content is 0.03 weight %, surplus is sterile water, and the pH of the culture medium is 7.2.(7) by lichens obtained in step (1) to (6) Bacillus, bacillus subtilis, bacillus pumilus, bacillus coagulans, saccharomyces cerevisiae and Streptomyces jingyangensis are proportionally It is mixed, in the microbial bacterial agent made, on the basis of total viable count of obtained microbial bacterial agent, bacillus licheniformis Viable count be 15 weight % of total viable count, the viable count of bacillus subtilis is the 15% of total viable count, short and small gemma bar The work that the viable count of bacterium is the 20% of total viable count, the viable count of bacillus coagulans is the 20% of total viable count, saccharomyces cerevisiae Bacterium number is the 15% of total viable count, the viable count of Streptomyces jingyangensis is the 15% of total viable count.
Embodiment 4
The present embodiment is for illustrating microbial bacterial agent of the present invention for the pre-fermented side of padding in fermentation bed cultivation Method.The present embodiment uses microbial bacterial agent same as Example 3.
The pine wood sawdust of 100 parts by weight and the rice husk of 66 parts by weight are taken, 20 parts by weight water are spilled into, is uniformly mixed and heap is good, After mixing by the wheat bran of 6 parts by weight and the microbial inoculum of 0.17 parts by weight, it is uniformly sprinkling upon on the sawdust and rice husk accumulated, by institute There is raw material to be fully mixed to substantially uniformity, while adjusting moisture to padding water content is 45%, mixed padding presses every heap 10m3, High 1.5m accumulates, and straw mattress heat-preservation fermentation is covered on padding, as 55 DEG C of padding temperature >, can be used after spreading out.
Embodiment 5
The present embodiment is for illustrating microbial bacterial agent of the present invention for the pre-fermented side of padding in fermentation bed cultivation Method.The present embodiment uses microbial bacterial agent same as Example 2.
The pine wood sawdust of 100 parts by weight and the rice husk of 60 parts by weight are taken, 20 parts by weight water are spilled into, is uniformly mixed and heap is good, After mixing by the wheat bran of 10 parts by weight and the microbial inoculum of 0.14 parts by weight, it is uniformly sprinkling upon on the sawdust and rice husk accumulated, it will All raw materials are fully mixed to substantially uniformity, while adjusting moisture to padding water content is 40%, and mixed padding presses every heap 10m3, high 1.5m accumulation covers straw mattress heat-preservation fermentation on padding, i.e. usable after spreading out as 55 DEG C of padding temperature >.
Embodiment 6
The present embodiment is for illustrating microbial bacterial agent of the present invention for the pre-fermented side of padding in fermentation bed cultivation Method.The present embodiment uses microbial bacterial agent same as Example 1.
The pine wood sawdust of 100 parts by weight and the rice husk of 70 parts by weight are taken, 20 parts by weight water are spilled into, is uniformly mixed and heap is good, After mixing by the microbial inoculum of 0.21 parts by weight, it is uniformly sprinkling upon on the sawdust and rice husk accumulated, all raw materials is sufficiently mixed To substantially uniformity, while adjusting moisture to padding water content is 50%, and mixed padding presses every heap 10m3, high 1.5m accumulation, pad Straw mattress heat-preservation fermentation is covered on material, as 55 DEG C of padding temperature >, can be used after spreading out.
Comparative example 3
Using the padding pre fermentation method in embodiment 4, the difference is that using microbial bacteria identical with comparative example 1 Agent.
Comparative example 4
This comparative example is used for the pad material fermentation method for illustrating to be different from the present invention, and uses microorganism identical with comparative example 2 Microbial inoculum.
(1) prepare padding according to following weight ratio: the dosage of rice straw is 20 weight %, and the dosage of rice husk is 30 weights % is measured, the dosage of sawdust is 40 weight %, and the dosage of rice bran is 10 weight %.(2) using microbial bacterial agent made from comparative example 2 The padding is inoculated with, relative to the base-material of 100 parts by weight, the inoculum concentration of microbial bacterial agent is 3 parts by weight.(3) it docks Padding after kind ferments, and fermentation temperature is 50 DEG C, and fermentation time is 2 days.(4) padding after fermentation is carried out in pig house Windrow, the height of windrow are 55 centimeters, and temperature maintains 35-60 DEG C in windrow, which is 150 days using the time, pig house Temperature maintain 15-35 DEG C.
Testing example 1
This testing example measures the ability of the degradation of ammonia nitrogen of the microbial bacterial agent of embodiment 1-3 and comparative example 1-2.
Prepare sterile beef-protein medium respectively, 100mL culture medium be packed into the conical flask of 250mL, respectively plus The microbial bacterial agent 0.05g for entering embodiment 1-3 and comparative example 1 is placed in 37 DEG C of cultures for 24 hours.
Prepare ammonia oxidation bacteria culture medium ((NH4)2SO42g/L, NaCl 0.3g/L, FeSO4·7H2O 0.03g/L, MgSO4·7H2O 0.03g/L, K2HPO41g/L, NaHCO31.6g/L, agar powder 18g/L use 1N sodium hydroxide solution tune For whole pH to 7.2), every bottle of 100mL accesses the above-mentioned each bacterium solution of 1mL.After culture 4 days, the concentration of ammonia nitrogen in culture medium is detected, as a result It is listed in table 1.
Table 1
According to the data of table 1 as it can be seen that deposit number of the invention is the bacillus licheniformis of CGMCC No.11235 (Bacillus licheniformis) and its microbial inoculum have the energy of degradation of ammonia nitrogen outstanding relative to other microorganisms and microbial inoculum Power.
Testing example 2
This testing example measures the acid producing ability of the microbial bacterial agent of embodiment 1-3 and comparative example 1-2.
Prepare sterile beef-protein medium respectively, 100mL culture medium be packed into the conical flask of 250mL, respectively plus The microbial bacterial agent 0.05g for entering embodiment 1-3 and comparative example 1 is placed in 37 DEG C of cultures for 24 hours.
Prepare acid-producing bacteria culture medium (glucose 6g/L, yeast extract 1g/L, peptone 1g/L, MgSO40.05g/L, CaCO3 1g/L, bromocresol purple 0.04g/L), acid-producing bacteria culture medium 8mL is placed in teat glass (18mm*180mm), sterilizing is good spare. The above-mentioned each bacterium solution of 1mL is accessed, 37 DEG C, 170r/min shake culture are placed in.It cultivates and sees whether that bromocresol purple is made to change colour for 24 hours afterwards, And the pH value in each sample tube is measured, as a result it is listed in table 2.
Table 2
Microbial inoculum Whether change colour pH
Embodiment 1 It is 5.12
Embodiment 2 It is 5.01
Embodiment 3 It is 4.93
Comparative example 1 It is no 7.09
Comparative example 2 It is no 6.83
According to the data of table 2 as it can be seen that deposit number of the invention is the bacillus licheniformis of CGMCC No.11235 (Bacillus licheniformis) and its microbial inoculum have stronger acid producing ability relative to other microorganisms and microbial inoculum, can be with Acidic environment needed for maintaining microorganism growth.
Testing example 3
This testing example measures the performance of fermentation bed in embodiment 4-6 and comparative example 3-4 padding pre fermentation method.Test PH value, temperature and the total number of bacteria of padding, are as a result listed in table 3 respectively, in table 4 and table 5.The test method of pH value are as follows: take quite In the air-dry sample of the fresh sample of 2g, add 50mL distilled water, vibrated in shaking table and mix 20min, stands 2h, measure the pH value of supernatant; The measuring method of temperature is thermometer to be inserted perpendicularly into from ramped surfaces to material heap center, in depth apart from 1 meter of ground eminence in material heap Degree is that long handle metallic thermometer measuring temperature is used at 30cm, 60cm and 90cm, is averaged;The measuring method of total number of bacteria is dilute Release spread plate method.
Table 3
According to the data of table 3 as it can be seen that microbial inoculum of the invention can buffer the pH value for adjusting fermentation padding, it is made to maintain phase To neutral condition, this is conducive to the flourish of probiotics, is conducive to quickly disappearing for feces of livestock and poultry when using in poultry house Solution.
Table 4
It can be seen that microbial inoculum of the invention according to the data of table 4 to be added in fermenting bed padding, fermentation calefaction speed is fast, Fermenting third day can be into the megathermal period, this illustrates that the bacteria fermentation speed is fast, high-efficient.From embodiment 4-5 and embodiment 6 Data comparison can be seen that preferred fermenting bed padding be sawdust, rice husk and wheat bran be used in conjunction with preparation fermentation bed in the case where, The fermenting speed of microbial inoculum is faster.
Table 5
According to the data of table 5 as it can be seen that compared with comparative example 3-4, it can be cultivated in microbial bacterial agent used in embodiment 4-6 Total number of bacteria is higher, and the deposit number wherein contained in microbial inoculum is the bacillus licheniformis of CGMCC No.11235 The predominant number of (Bacillus licheniformis), and the growth rate of the bacillus licheniformis in microbial inoculum is quickly, Miscellaneous bacteria in padding gradually tails off.This is because most der Pilz is killed at 55 DEG C of >, in addition there are many bacteriums miscellaneous Bacterium also non-refractory, but the bacillus licheniformis in microbial inoculum under the high temperature conditions can suspend mode become when after the megathermal period as padding In main bacteria seed.Can be seen that preferred fermenting bed padding from the data comparison of embodiment 4-5 and embodiment 6 is sawdust, rice In the case that shell and wheat bran are used in conjunction with preparation fermentation bed, the microbial inoculum bacterium in padding is more, and growth rate is also faster.
Testing example 4
The padding pre fermentation method that this testing example measures embodiment 4-6 and comparative example 3-4 is used for fermentation bed cultivation skill The effect of art.
Select identical age in days, Du × length × Dasanyuan of weight close (about 10kg) hybridizes porkling 100, according to weight and Principle similar in sex ratio is equally divided into 5 groups, and microbial bacterial agent identical with embodiment 4-6 and comparative example 3-4 is respectively adopted Fermentation bed is prepared with padding pre fermentation method.
Above-mentioned 5 groups of porklings feed 70 days, the growth performance of pig house environment situation and pig are tested and count, as a result such as 6 institute of table Show.
Table 6
Group Colony house situation Pig growing state
Embodiment 4 Without obvious stink Hair color is bright, and pig body is clean
Embodiment 5 Without obvious stink Hair color is bright, and pig body is clean
Embodiment 6 Without obvious stink Hair color is bright, and pig body is clean
Comparative example 3 Stench Dull, pig body are dirtier
Comparative example 4 There is stink Dull, pig body are dirtier
The colony house environment that can be seen that embodiment 4-6 according to the data of table 6 is obviously better than comparative example 3-4, illustrates using this The microbial bacterial agent of invention can efficiently eliminate cultivation stench, improve the environment of cultivation colony house, reduce stink to environmental emission.
Table 7
Group First weight/kg Last weight/kg Daily gain/kg Feedstuff-meat ratio
Embodiment 4 10.56±1.45 52.47±5.09 0.60±0.03 2.34
Embodiment 5 10.37±1.53 51.67±4.15 0.59±0.04 2.38
Embodiment 6 10.41±1.48 51.01±4.15 0.58±0.04 2.41
Comparative example 3 10.44±1.22 44.57±4.23 0.49±0.04 2.88
Comparative example 4 10.48±1.22 47.70±4.23 0.53±0.04 2.64
It can be seen that the various aspects growths such as daily gain and the feedstuff-meat ratio that pig is cultivated in embodiment 4-6 according to the data of table 7 The cultivation pig that can be substantially better than in comparative example 3-4, this is because disappearing after microbial bacterial agent of the invention is applied to fermentation bed cultivation In addition to cultivating colony house foul smell, so that the growing environment for cultivating pig is greatly improved, promote the growth of cultivation pig, improve the effect of cultivation Benefit.Can be seen that preferred fermenting bed padding from the data comparison of embodiment 4-5 and embodiment 6 is that sawdust, rice husk and wheat bran are total In the case where using preparation fermentation bed, the growth performance for cultivating pig is more excellent.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally The thought of invention, it should also be regarded as the disclosure of the present invention.

Claims (10)

1. a kind of bacillus licheniformis, it is characterised in that: the guarantor of the bacillus licheniformis (Bacillus licheniformis) Hiding number is CGMCC No.11235.
2. a kind of microbial bacterial agent, which contains thallus and culture medium, it is characterised in that: the thallus is preservation volume Number be CGMCC No.11235 bacillus licheniformis.
3. microbial bacterial agent according to claim 2, it is characterised in that: in every gram of microbial bacterial agent, deposit number Viable count for the bacillus licheniformis of CGMCC No.11235 is 109-1010cfu。
4. microbial bacterial agent according to claim 2 or 3, it is characterised in that: the culture medium is beef extract-peptone training Support at least one of base, nutrient broth medium and LB culture medium.
5. microbial bacterial agent described in any one of bacillus licheniformis described in claim 1 and claim 2-4 is being sent out Application in the cultivation of ferment bed.
6. 5 application according to claim, it is characterised in that: the fermentation bed contains padding and the microbial bacterial agent, Wherein, the weight ratio of microbial bacterial agent and padding is 1:(900-1200).
7. application according to claim 5 or 6, it is characterised in that: the moisture content of the padding is 40-50 weight %.
8. application according to claim 5 or 6, it is characterised in that: the padding is in sawdust, rice husk and wheat bran It is one or more of.
9. application according to claim 8, it is characterised in that: relative to the sawdust of 100 parts by weight, the dosage of rice husk is 60-70 parts by weight, the dosage of wheat bran are 5-10 parts by weight.
10. application according to claim 5, it is characterised in that: the fermentation bed cultivation includes fermentation bed to raise pig, fermentation bed At least one of poultry, fermentation bed duck culturing and fermentation bed sheep raising.
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CN107502572B (en) * 2017-09-08 2020-08-21 中国农业科学院农业环境与可持续发展研究所 Application of bacillus licheniformis in straw degradation, microbial agent containing bacillus licheniformis and application of bacillus licheniformis

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