CN110438031A - A kind of microbial deoderizer and preparation method thereof - Google Patents
A kind of microbial deoderizer and preparation method thereof Download PDFInfo
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- CN110438031A CN110438031A CN201910571160.4A CN201910571160A CN110438031A CN 110438031 A CN110438031 A CN 110438031A CN 201910571160 A CN201910571160 A CN 201910571160A CN 110438031 A CN110438031 A CN 110438031A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention belongs to microorganism formulation technical fields, and in particular to a kind of microbial deoderizer and preparation method thereof.Microbial deoderizer of the invention includes saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid, it is seeded in fluid nutrient medium and cultivates after saccharomycete, acetic acid bacteria and sulfur oxidizing bacterium are activated respectively, it inoculates to fermentation cylinder for fermentation, by 2-4:1:1-2 is mixed by volume after the dilution of respective fermentation liquid to obtain the final product.There is good synergistic effect between the various bacterial strains of microbial deoderizer selection of the invention, it efficiently can synergistically absorb and remove the ammonia in foul smell and hydrogen sulfide, rubbish surface is directly sprayed on when use, 90% and 80% or more is averagely respectively reached to the removal rate of ammonia, hydrogen sulfide in foul smell in rigid application, removal rate still averagely reaches 50% and 40% or more after application 6h, instantaneous good deodorization effect, the deodorization duration is long, and environmentally friendly.
Description
Technical field
The invention belongs to microorganism formulation technical fields, and in particular to a kind of microbial deoderizer and preparation method thereof.
Background technique
House refuse generally can be divided into four major class: recyclable rubbish, kitchen garbage, Harmful Waste and other rubbish.Resident
The soot that house refuse is stacked in cell or campus can generate the summer of many foul gas, especially high temperature and humidity.Life
Rubbish can ferment in stacking process generate its main ingredient of foul smell be hydrogen sulfide and ammonia, in addition there are methyl mercaptan, methylamine,
The organic gas such as methyl sulphur.When amount of oxygen is enough, organic substance such as protein in rubbish generates under aerobic bacteria effect
Ammonia；In oxygen deficiency, organic matter is decomposed into halfway oxidation product hydrogen sulfide, sulfur dioxide, mercaptan by anaerobic bacteria
The compounds such as class, amine, these gaseous volatiles are larger, easily spread in an atmosphere, and portion gas is toxic, penetrating odor
Greatly.It is mostly organic matter outside the vulcanisation hydrogen of these foul gas and ammonia, not only pathophorous insect, bacterium can be promoted a large amount of
Procreation and also sucking human body after to respiratory system, nervous system, the circulatory system, endocrine system generate strong impulse make
With.The symptoms such as be sick in the stomach, vomit can be made one in short time, a large amount of sucking may result in endocrine disorder, the heart for a long time
The serious conditions such as cranial vascular disease.
The Study on treatment technology of foul gas is very fast with the development of the states such as Japan, Holland, the U.S..Currently, being gone for stink
Except method mainly has: sense organ deodorization process, chemical deodorization method, physical deodorization method and biological odor removal method.Feel deodorization and method with strongly
Fragrance ingredient make foul gas not be perceived out, the stink odor being harmful to the human body can not be effectively eliminated, foul smell is practical
On still remain.Chemical deodorizing agent significant effect but higher cost, and used chemical raw material has human body and animal
Certain toxicity.Plant deodorant extraction process is complicated, and production cost is high, therefore in intensive manufacture and promotes the use of process
In receive restriction.
Summary of the invention
Against the above technical problems, the object of the present invention is to provide a kind of microbial deoderizers and preparation method thereof, should
Microbial deoderizer selection saccharomycete, acetic acid bacteria and sulfur oxidizing bacterium are formulated by fermentation, can be used for removing for house refuse
It is smelly, it can significantly absorb and remove the NH in foul smell3And H2S can reduce odor concentration for a long time.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of microbial deoderizer, the microbial deoderizer are to include saccharomycetes to make fermentation liquid, acetic acid fermented liquid
With the mixed bacteria liquid of sulfur oxidizing bacterium fermentation liquid, the saccharomycete is saccharomyces cerevisiae, and bacterial strain deposit number is CGMCC No.5449,
The saccharomycetes to make fermentation liquid, which passes through activation culture for saccharomyces cerevisiae and is seeded to fermentation cylinder for fermentation, to be made, and the acetic acid bacteria is vinegar
Acidfast bacilli or gluconacetobacter, wherein the deposit number of acetobacter is CCTCC AB 2016191, the preservation of gluconacetobacter
Number is CCTCC AB 204052, and the acetic acid fermented liquid is acetobacter or gluconacetobacter by activation culture and connects
Kind to fermentation cylinder for fermentation is made, and the sulfur oxidizing bacterium is happiness temperature acid sulphur rod bacterium, and bacterial strain deposit number is CGMCC
No.14008, the sulfur oxidizing bacterium fermentation liquid are the sour sulphur rod bacterium of happiness temperature by activation culture and are seeded to fermentation cylinder for fermentation system
, the volume ratio of the saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 2-4:1:1-2, the mixing
The dense bacterium of saccharomycete, acetic acid bacteria and sulfur oxidizing bacterium in bacterium solution is 1 × 107- 1 × 108CFU/mL。
Preferably, the volume ratio of the saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 4:1:2.
A kind of preparation method of microbial deoderizer, comprising the following steps:
(1) it prepares saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium and cultivate after saccharomycete is activated, then connect
It plants to fermentation cylinder for fermentation；
(2) it prepares acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium and cultivate after acetic acid bacteria is activated, then connect
It plants to fermentation cylinder for fermentation；
(3) it prepares sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium and cultivate after sulfur oxidizing bacterium is activated,
Then it is seeded to fermentation cylinder for fermentation；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by bacterium
Concentration is adjusted to 1 × 107- 1 × 108CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are pressed to the volume of 2-4:1:1-2
Than be uniformly mixed to get arrive the microbial deoderizer.
Further, in the step (1) saccharomycetes to make fermentation liquid preparation method specifically: be inoculated with after activating saccharomycete
Into saccharomycete fluid nutrient medium, at 30 DEG C, 24-36h is cultivated in 170r/min shaking table, the level-one of saccharomycete is prepared
Then first order seed is inoculated into fermentation cylinder for fermentation by the inoculum concentration of 5-10%, wherein fermentation are as follows: temperature by seed
28-35 DEG C, speed of agitator 300-500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferments 36-60h to get the ferment is arrived
Female fermented liquid.
Further, the ingredient of the saccharomycete fluid nutrient medium includes the glucose that mass fraction is 1.0%, quality point
The yeast extract that number is 1.0%, the sodium chloride that mass fraction is 0.1%, remaining is water, adjustment pH value to 5.5.
Further, in the step (2) acetic acid fermented liquid preparation method specifically: be inoculated with after activating acetic acid bacteria
Into acetic acid bacteria fluid nutrient medium, at 30 DEG C, 24-36h is cultivated in 170r/min shaking table, the level-one of acetic acid bacteria is prepared
Then first order seed is inoculated into fermentation cylinder for fermentation by the inoculum concentration of 5-10%, wherein fermentation are as follows: temperature by seed
30-35 DEG C, speed of agitator 300-500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferments 48-60h to get the vinegar is arrived
Acid bacteria fermentation liquid.
Further, the ingredient of the acetic acid bacteria fluid nutrient medium includes the glucose that mass fraction is 1.0%, quality point
The yeast extract that number is 1.0%, the sodium chloride that mass fraction is 0.1%, remaining is water, adjustment pH value to 5.5.
Further, when the acetic acid bacteria is inoculated into fermentation cylinder for fermentation, it is 3.0% that mass fraction, which is added, in the fermentation later period
Dehydrated alcohol.
Further, in the step (3) sulfur oxidizing bacterium fermentation liquid preparation method specifically: after sulfur oxidizing bacterium is activated
It is inoculated into sulfur oxidizing bacterium fluid nutrient medium, at 45 DEG C, cultivates 24-36h in 170r/min shaking table, acetic acid bacteria is prepared
First order seed, first order seed is then inoculated into fermentation cylinder for fermentation by the inoculum concentration of 5-10%, wherein fermentation
Are as follows: 30-35 DEG C of temperature, speed of agitator 300-500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferments 48-60h to get arriving
The sulfur oxidizing bacterium fermentation liquid.
Further, the ingredient of the sulfur oxidizing bacterium fluid nutrient medium includes the sulphur powder that mass fraction is 0.2%, K2HPO4
0.5g/L, Ca (NO3)2·4H2O 0.01g/L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L steams
Distilled water 1000ml, adjustment pH value to 2.5.
Further, the microbial deoderizer that the present invention is prepared can directly be sprayed on house refuse surface.
Compared with prior art, the invention has the following beneficial effects:
There is good synergistic effect between the various bacterial strains selected in microbial deoderizer of the invention, will not generate
Antagonism, compounding together can good symbiosis, efficiently can synergistically absorb and remove the NH in foul smell3And H2S.Microorganism is removed
When smelly microbial inoculum is sprayed on house refuse surface, the saccharomycete in microbial deoderizer contains higher than 3-10 times of other microorganisms
Superoxide dismutase, can ultra-oxygen anion free radical (O effectively in catalytic body2-) it is converted into harmless H2O2And O2, release O2-
Toxic action to body.Saccharomycete available amino acid, carbohydrate and various organic substances are generated by fermentation and promote cell point
The production of the active material split, matrix needed for being proliferated for other effective microbes provides nutrition assurance, promotes other beneficial to micro- life
The growth of object, and fragrance can also be generated.Acetic acid bacteria can produce a large amount of organic acids, formed and be unfavorable for putrefactive microorganisms life
Acidic environment effectively inhibits harmful microbe activity, and then inhibits the release of foul gas, produces when fundamentally degradation is decomposed
The substance of raw foul gas；Sulfur oxidizing bacterium energy rapid oxidation decomposing hydrogen sulfide, reduces the volatilization and release of hydrogen sulfide gas, in turn
Reduce the generation of foul gas.Microbial deoderizer preparation prepared by the present invention is simple, and good deodorization effect, the duration is long,
And it is environmentally protective, it is environmentally friendly.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with the embodiment of the present invention, to this hair
Technical solution in bright embodiment is clearly and completely described, it is clear that described embodiment is that a part of the invention is implemented
Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creativeness
Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of labour.
Glucose used in following embodiment, yeast extract, sodium chloride, sulphur powder, K2HPO4、Ca(NO3)2·4H2O、
KCl、MgSO4·7H2O and (NH4)2SO4It is commercially available.The saccharomycete is saccharomyces cerevisiae, and bacterial strain deposit number is CGMCC
No.5449, the acetic acid bacteria are acetobacter or gluconacetobacter, and wherein the deposit number of acetobacter is CCTCC AB
2016191, the deposit number of gluconacetobacter is CCTCC AB204052, and the sulfur oxidizing bacterium is the sour sulphur rod bacterium of happiness temperature, bacterium
Strain deposit number is CGMCC No.14008.
Embodiment 1
A kind of microbial deoderizer, including saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid, it is described
Saccharomycete is saccharomyces cerevisiae, and the acetic acid bacteria is acetobacter, and the sulfur oxidizing bacterium is the sour sulphur rod bacterium of happiness temperature, the saccharomycete
The volume ratio of fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 2:1:1.
A kind of preparation method of microbial deoderizer, comprising the following steps:
(1) preparation of saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium after saccharomycete is activated, the yeast
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates in 170r/min shaking table for 24 hours,
The first order seed of saccharomycete is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein sending out
Ferment condition control are as follows: 28 DEG C of temperature, speed of agitator 500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferments 36h to get arriving
The saccharomycetes to make fermentation liquid；
(2) preparation of acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium after acetic acid bacteria is activated, the acetic acid
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates 36h in 170r/min shaking table,
The first order seed of acetic acid bacteria is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein
Fermentation are as follows: 30 DEG C of temperature, speed of agitator 300r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 48h, fermentation
The dehydrated alcohol that mass fraction is 3.0% is added to get the acetic acid fermented liquid is arrived in later period；
(3) preparation of sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium after sulfur oxidizing bacterium is activated, institute
State sulfur oxidizing bacterium fluid nutrient medium ingredient include mass fraction be 0.2% sulphur powder, K2HPO40.5g/L, Ca (NO3)2·4H2O
0.01g/L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L, distilled water 1000ml, adjustment pH value is extremely
2.5, at 45 DEG C, the first order seed that sulfur oxidizing bacterium is prepared for 24 hours is cultivated in 170r/min shaking table, then by level-one kind
Son is inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein fermentation are as follows: and 30 DEG C of temperature, speed of agitator 300r/
Min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 60h to get the sulfur oxidizing bacterium fermentation liquid is arrived；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by bacterium
Concentration is adjusted to 1 × 107CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are mixed by the volume ratio of 2:1:1
Close uniformly to get arrive the microbial deoderizer.
Embodiment 2
A kind of microbial deoderizer, including saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid, it is described
Saccharomycete is saccharomyces cerevisiae, and the acetic acid bacteria is gluconacetobacter, and the sulfur oxidizing bacterium is the sour sulphur rod bacterium of happiness temperature, the yeast
The volume ratio of fermented liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 2:1:2.
A kind of preparation method of microbial deoderizer, comprising the following steps:
(1) preparation of saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium after saccharomycete is activated, the yeast
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates 36h in 170r/min shaking table,
The first order seed of saccharomycete is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein
Fermentation are as follows: 35 DEG C of temperature, speed of agitator 400r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 48h to get
To the saccharomycetes to make fermentation liquid；
(2) preparation of acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium after acetic acid bacteria is activated, the acetic acid
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates in 170r/min shaking table for 24 hours,
The first order seed of acetic acid bacteria is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein sending out
Ferment condition control are as follows: 35 DEG C of temperature, speed of agitator 400r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 60h, after fermentation
The dehydrated alcohol that mass fraction is 3.0% is added to get the acetic acid fermented liquid is arrived in phase；
(3) preparation of sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium after sulfur oxidizing bacterium is activated, institute
State sulfur oxidizing bacterium fluid nutrient medium ingredient include mass fraction be 0.2% sulphur powder, K2HPO40.5g/L, Ca (NO3)2·4H2O
0.01g/L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L, distilled water 1000ml, adjustment pH value is extremely
2.5, at 45 DEG C, 36h is cultivated in 170r/min shaking table, the first order seed of sulfur oxidizing bacterium is prepared, then by level-one kind
Son is inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein fermentation are as follows: 35 DEG C of temperature, speed of agitator is
400r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 48h to get the sulfur oxidizing bacterium fermentation liquid is arrived；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by bacterium
Concentration is adjusted to 1 × 108CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are mixed by the volume ratio of 2:1:2
Close uniformly to get arrive the microbial deoderizer.
Embodiment 3
A kind of microbial deoderizer, including saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid, it is described
Saccharomycete is saccharomyces cerevisiae, and the acetic acid bacteria is acetobacter, and the sulfur oxidizing bacterium is the sour sulphur rod bacterium of happiness temperature, the saccharomycete
The volume ratio of fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 3:1:1.
A kind of preparation method of microbial deoderizer, comprising the following steps:
(1) preparation of saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium after saccharomycete is activated, the yeast
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates in 170r/min shaking table for 24 hours,
The first order seed of saccharomycete is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein sending out
Ferment condition control are as follows: 35 DEG C of temperature, speed of agitator 300r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferments 60h to get arriving
The saccharomycetes to make fermentation liquid；
(2) preparation of acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium after acetic acid bacteria is activated, the acetic acid
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates 36h in 170r/min shaking table,
The first order seed of acetic acid bacteria is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein
Fermentation are as follows: 30 DEG C of temperature, speed of agitator 500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 60h, fermentation
The dehydrated alcohol that mass fraction is 3.0% is added to get the acetic acid fermented liquid is arrived in later period；
(3) preparation of sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium after sulfur oxidizing bacterium is activated, institute
State sulfur oxidizing bacterium fluid nutrient medium ingredient include mass fraction be 0.2% sulphur powder, K2HPO40.5g/L, Ca (NO3)2·4H2O
0.01g/L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L, distilled water 1000ml, adjustment pH value is extremely
2.5, at 45 DEG C, the first order seed that sulfur oxidizing bacterium is prepared for 24 hours is cultivated in 170r/min shaking table, then by level-one kind
Son is inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein fermentation are as follows: 30 DEG C of temperature, speed of agitator is
500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 48h to get the sulfur oxidizing bacterium fermentation liquid is arrived；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by bacterium
Concentration is adjusted to 1 × 107CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are mixed by the volume ratio of 3:1:1
Close uniformly to get arrive the microbial deoderizer.
Embodiment 4
A kind of microbial deoderizer, including saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid, it is described
Saccharomycete is saccharomyces cerevisiae, and the acetic acid bacteria is gluconacetobacter, and the sulfur oxidizing bacterium is the sour sulphur rod bacterium of happiness temperature, the yeast
The volume ratio of fermented liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 3:1:2.
A kind of preparation method of microbial deoderizer, comprising the following steps:
(1) preparation of saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium after saccharomycete is activated, the yeast
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates 36h in 170r/min shaking table,
The first order seed of saccharomycete is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein
Fermentation are as follows: 28 DEG C of temperature, speed of agitator 300r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 48h to get
To the saccharomycetes to make fermentation liquid；
(2) preparation of acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium after acetic acid bacteria is activated, the acetic acid
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates in 170r/min shaking table for 24 hours,
The first order seed of acetic acid bacteria is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein sending out
Ferment condition control are as follows: 35 DEG C of temperature, speed of agitator 300r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 48h, after fermentation
The dehydrated alcohol that mass fraction is 3.0% is added to get the acetic acid fermented liquid is arrived in phase；
(3) preparation of sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium after sulfur oxidizing bacterium is activated, institute
State sulfur oxidizing bacterium fluid nutrient medium ingredient include mass fraction be 0.2% sulphur powder, K2HPO40.5g/L, Ca (NO3)2·4H2O
0.01g/L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L, distilled water 1000ml, adjustment pH value is extremely
2.5, at 45 DEG C, 36h is cultivated in 170r/min shaking table, the first order seed of sulfur oxidizing bacterium is prepared, then by level-one kind
Son is inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein fermentation are as follows: and 35 DEG C of temperature, speed of agitator 300r/
Min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 60h to get the sulfur oxidizing bacterium fermentation liquid is arrived；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by bacterium
Concentration is adjusted to 1 × 108CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are mixed by the volume ratio of 3:1:2
Close uniformly to get arrive the microbial deoderizer.
Embodiment 5
A kind of microbial deoderizer, including saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid, it is described
Saccharomycete is saccharomyces cerevisiae, and the acetic acid bacteria is acetobacter, and the sulfur oxidizing bacterium is the sour sulphur rod bacterium of happiness temperature, the saccharomycete
The volume ratio of fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 4:1:1.
A kind of preparation method of microbial deoderizer, comprising the following steps:
(1) preparation of saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium after saccharomycete is activated, the yeast
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates in 170r/min shaking table for 24 hours,
The first order seed of saccharomycete is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein
Fermentation are as follows: 28 DEG C of temperature, speed of agitator 400r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 60h to get
To the saccharomycetes to make fermentation liquid；
(2) preparation of acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium after acetic acid bacteria is activated, the acetic acid
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates 36h in 170r/min shaking table,
The first order seed of acetic acid bacteria is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein sending out
Ferment condition control are as follows: 30 DEG C of temperature, speed of agitator 400r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 48h, after fermentation
The dehydrated alcohol that mass fraction is 3.0% is added to get the acetic acid fermented liquid is arrived in phase；
(3) preparation of sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium after sulfur oxidizing bacterium is activated, institute
State sulfur oxidizing bacterium fluid nutrient medium ingredient include mass fraction be 0.2% sulphur powder, K2H PO40.5g/L, Ca (NO3)2·
4H2O 0.01g/L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L, distilled water 1000ml adjust pH
Value at 45 DEG C, cultivates the first order seed that sulfur oxidizing bacterium is prepared for 24 hours, then by one to 2.5 in 170r/min shaking table
Grade seed by 5% inoculum concentration is inoculated into fermentation cylinder for fermentation, wherein fermentation are as follows: 30 DEG C of temperature, speed of agitator is
400r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 48h to get the sulfur oxidizing bacterium fermentation liquid is arrived；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by bacterium
Concentration is adjusted to 1 × 107CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are mixed by the volume ratio of 4:1:1
Close uniformly to get arrive the microbial deoderizer.
Embodiment 6
A kind of microbial deoderizer, including saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid, it is described
Saccharomycete is saccharomyces cerevisiae, and the acetic acid bacteria is gluconacetobacter, and the sulfur oxidizing bacterium is the sour sulphur rod bacterium of happiness temperature, the yeast
The volume ratio of fermented liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 4:1:2.
A kind of preparation method of microbial deoderizer, comprising the following steps:
(1) preparation of saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium after saccharomycete is activated, the yeast
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates 36h in 170r/min shaking table,
The first order seed of saccharomycete is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 5% inoculum concentration, wherein sending out
Ferment condition control are as follows: 35 DEG C of temperature, speed of agitator 500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferments 36h to get arriving
The saccharomycetes to make fermentation liquid；
(2) preparation of acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium after acetic acid bacteria is activated, the acetic acid
The ingredient of bacteria liquid culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, quality
The sodium chloride that score is 0.1%, remaining is water, adjusts pH value to 5.5, at 30 DEG C, cultivates in 170r/min shaking table for 24 hours,
The first order seed of acetic acid bacteria is prepared, first order seed is then inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein
Fermentation are as follows: 35 DEG C of temperature, speed of agitator 500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa, ferment 60h, fermentation
The dehydrated alcohol that mass fraction is 3.0% is added to get the acetic acid fermented liquid is arrived in later period；
(3) preparation of sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium after sulfur oxidizing bacterium is activated, institute
State sulfur oxidizing bacterium fluid nutrient medium ingredient include mass fraction be 0.2% sulphur powder, K2HPO40.5g/L, Ca (NO3)2·4H2O
0.01g/L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L, distilled water 1000ml, adjustment pH value is extremely
2.5, at 45 DEG C, 36h is cultivated in 170r/min shaking table, the first order seed of sulfur oxidizing bacterium is prepared, then by level-one kind
Son is inoculated into fermentation cylinder for fermentation by 10% inoculum concentration, wherein fermentation are as follows: 35 DEG C of temperature, speed of agitator is
500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 60h to get the sulfur oxidizing bacterium fermentation liquid is arrived；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by bacterium
Concentration is adjusted to 1 × 108CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are mixed by the volume ratio of 4:1:2
Close uniformly to get arrive the microbial deoderizer.
Comparative example 1 is the difference from embodiment 1 is that only include saccharomycetes to make fermentation liquid in deodorizing microorganism.
Comparative example 2 is the difference from embodiment 1 is that only include acetic acid fermented liquid in deodorizing microorganism.
Comparative example 3 is the difference from embodiment 1 is that only include sulfur oxidizing bacterium fermentation liquid in deodorizing microorganism.
Comparative example 4 is the difference from embodiment 1 is that only include saccharomycetes to make fermentation liquid and acetic acid fermented liquid in deodorizing microorganism.
Comparative example 5 in deodorizing microorganism comprising saccharomycetes to make fermentation liquid and sulfur oxidizing bacterium the difference from embodiment 1 is that only ferment
Liquid.
Comparative example 6 in deodorizing microorganism comprising acetic acid fermented liquid and sulfur oxidizing bacterium the difference from embodiment 1 is that only ferment
Liquid.
In Changsha Yuelu District, Hunan Normal University tree carries out live Deodorization Experiment, experimental day temperature up to institute soot
It is 36 DEG C, 3 grades of wind speed is hereinafter, monitoring project is mainly refuse odor source NH3And H2S.By 1-6 of the embodiment of the present invention and comparison
Example 1-3 dilutes 10 times, sprays in soot by 1L/m3.Every two hours with Ke An labour protection New Tech S. R. L., Beijing after sprinkling
Air sampler instrument QC-3 and atmospheric sampling bag sampling, measure NH3And H2S concentration.
Test method: according to the GB/T14668-93:NH of Detection of Air Quality3Detection use reagent colorimetric method；
GB11060.2-89:H2The detection of S uses methylene-blue colorimetric method.Hydrogen sulfide and ammonia rate of descent are detected, to detect deodorization
Effect.
Deodorizing microorganism compares the removal rate of foul smell and lasting deodorizing effect under 1 different disposal of table
From the data in table 1, it can be seen that the deodorizing microorganism prepared in 1-6 of the embodiment of the present invention is sprayed at after being diluted with water 10 times
Rubbish surface averagely respectively reaches 90% and 80% or more to the removal rate of ammonia, hydrogen sulfide in foul smell in rigid application, applies
With after 6h 50% and 40% or more is still averagely reached to the removal rate of ammonia, hydrogen sulfide in foul smell, wherein embodiment 6 is removed
Smelly effect is best.
Single bacterial strain fermentation liquor is used only in deodorizing microorganism in comparative example 1-3, wherein best to ammonia removal effect
For the comparative example 2 for only including acetic acid fermented liquid, removal rate 38.4%；Best to hydrogen sulfide removal effect is only comprising sulphur
To ammonia and sulphur after the deodorizing microorganism application 6h of the comparative example 3 of oxidation fermented liquid, removal rate 31.3%, and comparative example 1-3
The removal rate highest for changing hydrogen only has 11.6% and 12.5%, to the removal rate of foul smell and lasting deodorization effect compared with embodiment 1-6
Fruit is obviously poor.
Deodorizing microorganism in comparative example 4-6 is mixed with using the fermentation liquid of two kinds of bacterial strains, wherein going to ammonia
It is the comparative example 4 for only including saccharomycetes to make fermentation liquid and acetic acid fermented liquid, removal rate 51.2% except effect is best；To vulcanization
Best hydrogen removal effect is the comparative example 5 for only including sulfur oxidizing bacterium fermentation liquid, removal rate 35.7%, and comparative example 4-6
Only has 12.4% and 11.7% to the removal rate highest of ammonia and hydrogen sulfide after deodorizing microorganism application 6h, compared with embodiment 1-6
Removal rate and lasting deodorizing effect to foul smell is obviously poor.
The above result shows that between each microbial bacteria there is good collaboration to make in the deodorizing microorganism of embodiment 1-6 preparation
With the removal rate to ammonia and hydrogen sulfide, and the instantaneous good deodorization effect of microbial bacterial agent of the invention, deodorization can be significantly improved
Duration is long, and environmentally friendly.
Claims (10)
1. a kind of microbial deoderizer, which is characterized in that the microbial deoderizer is to include saccharomycetes to make fermentation liquid, acetic acid
The mixed bacteria liquid of fermented liquid and sulfur oxidizing bacterium fermentation liquid, the saccharomycetes to make fermentation liquid are saccharomyces cerevisiae by activation culture and connect
Kind to fermentation cylinder for fermentation is made, and the acetic acid fermented liquid is acetobacter or gluconacetobacter by activation culture and is inoculated with
It is made to fermentation cylinder for fermentation, the sulfur oxidizing bacterium fermentation liquid is the sour sulphur rod bacterium of happiness temperature by activation culture and is seeded to fermentation
It ferments and is made in tank, the volume ratio of the saccharomycetes to make fermentation liquid, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid is 2-4:1:1-
2, the dense bacterium of saccharomycete, acetic acid bacteria and sulfur oxidizing bacterium in the mixed bacteria liquid is 1 × 107- 1 × 108CFU/mL。
2. a kind of microbial deoderizer according to claim 1, which is characterized in that the saccharomycetes to make fermentation liquid, acetic acid
The volume ratio of fermented liquid and sulfur oxidizing bacterium fermentation liquid is 4:1:2.
3. a kind of preparation method of microbial deoderizer as described in claim 1, which comprises the following steps:
(1) it prepares saccharomycetes to make fermentation liquid: being inoculated into saccharomycete fluid nutrient medium and cultivate after saccharomycete is activated, be then seeded to
Fermentation cylinder for fermentation；
(2) it prepares acetic acid fermented liquid: being inoculated into acetic acid bacteria fluid nutrient medium and cultivate after acetic acid bacteria is activated, be then seeded to
Fermentation cylinder for fermentation；
(3) it prepares sulfur oxidizing bacterium fermentation liquid: being inoculated into sulfur oxidizing bacterium fluid nutrient medium and cultivate after sulfur oxidizing bacterium is activated, then
It is seeded to fermentation cylinder for fermentation；
(4) by yeast fermentation broth, acetic acid fermented liquid and sulfur oxidizing bacterium fermentation liquid after counting with sterile water by the concentration of bacterium
It adjusts to 1 × 107- 1 × 108CFU/mL；
(5) yeast fermentation broth, acetic acid fermented liquid and the sulfur oxidizing bacterium fermentation liquid after dilution are mixed by the volume ratio of 2-4:1:1-2
Close uniformly to get arrive the microbial deoderizer.
4. a kind of preparation method of microbial deoderizer according to claim 3, which is characterized in that the step (1)
The preparation method of middle saccharomycetes to make fermentation liquid specifically: be inoculated into after activating saccharomycete in saccharomycete fluid nutrient medium, at 30 DEG C
Under, 24-36h is cultivated in 170r/min shaking table, the first order seed of saccharomycete is prepared, first order seed is then pressed into 5-10%
Inoculum concentration be inoculated into fermentation cylinder for fermentation, wherein fermentation are as follows: 28-35 DEG C of temperature, speed of agitator 300-500r/
Min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 36-60h to get the saccharomycetes to make fermentation liquid is arrived.
5. a kind of preparation method of microbial deoderizer according to claim 4, which is characterized in that the yeast liquid
The ingredient of body culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, mass fraction
For 0.1% sodium chloride, remaining is water, adjustment pH value to 5.5.
6. a kind of preparation method of microbial deoderizer according to claim 3, which is characterized in that the step (2)
The preparation method of middle acetic acid fermented liquid specifically: be inoculated into after activating acetic acid bacteria in acetic acid bacteria fluid nutrient medium, at 30 DEG C
Under, 24-36h is cultivated in 170r/min shaking table, the first order seed of acetic acid bacteria is prepared, first order seed is then pressed into 5-10%
Inoculum concentration be inoculated into fermentation cylinder for fermentation, wherein fermentation are as follows: 30-35 DEG C of temperature, speed of agitator 300-500r/
Min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 48-60h to get the acetic acid fermented liquid is arrived.
7. a kind of preparation method of microbial deoderizer according to claim 6, which is characterized in that the acetic acid bacterium solution
The ingredient of body culture medium includes the glucose that mass fraction is 1.0%, the yeast extract that mass fraction is 1.0%, mass fraction
For 0.1% sodium chloride, remaining is water, adjustment pH value to 5.5.
8. a kind of preparation method of microbial deoderizer according to claim 6, which is characterized in that the acetic acid bacteria connects
When kind arrives fermentation cylinder for fermentation, the dehydrated alcohol that mass fraction is 3.0% is added in the fermentation later period.
9. a kind of preparation method of microbial deoderizer according to claim 3, which is characterized in that the step (3)
The preparation method of middle sulfur oxidizing bacterium fermentation liquid specifically: it is inoculated into after activating sulfur oxidizing bacterium in sulfur oxidizing bacterium fluid nutrient medium,
At 45 DEG C, 24-36h is cultivated in 170r/min shaking table, the first order seed of acetic acid bacteria is prepared, then presses first order seed
The inoculum concentration of 5-10% is inoculated into fermentation cylinder for fermentation, wherein fermentation are as follows: 30-35 DEG C of temperature, speed of agitator is
300-500r/min, dissolved oxygen amount 80%, pressure 0.1Mpa ferment 48-60h to get the sulfur oxidizing bacterium fermentation liquid is arrived.
10. a kind of preparation method of microbial deoderizer according to claim 9, which is characterized in that the sulphur oxidation
The ingredient of bacteria liquid culture medium includes the sulphur powder that mass fraction is 0.2%, K2HPO40.5g/L, Ca (NO3)2·4H2O 0.01g/
L, KCl 0.1g/L, MgSO4·7H2O 0.5g/L, (NH4)2SO43.0g/L, distilled water 1000ml, adjustment pH value to 2.5.
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