CN112322522A - Environment-friendly enzyme resistant to alkaline environment of pig farm liquid dung and preparation method thereof - Google Patents
Environment-friendly enzyme resistant to alkaline environment of pig farm liquid dung and preparation method thereof Download PDFInfo
- Publication number
- CN112322522A CN112322522A CN202011155223.7A CN202011155223A CN112322522A CN 112322522 A CN112322522 A CN 112322522A CN 202011155223 A CN202011155223 A CN 202011155223A CN 112322522 A CN112322522 A CN 112322522A
- Authority
- CN
- China
- Prior art keywords
- environment
- friendly
- pig farm
- ferment
- alkaline environment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000003608 fece Anatomy 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 title abstract description 27
- 102000004190 Enzymes Human genes 0.000 title abstract description 27
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 7
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 7
- 239000004310 lactic acid Substances 0.000 claims abstract description 7
- 239000010871 livestock manure Substances 0.000 claims description 13
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 235000015278 beef Nutrition 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 150000007524 organic acids Chemical class 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000013543 active substance Substances 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 37
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 11
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 7
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 241000235342 Saccharomycetes Species 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000004332 deodorization Methods 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 4
- 229910021529 ammonia Inorganic materials 0.000 description 11
- 241000282887 Suidae Species 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 150000003278 haem Chemical class 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- BMUDPLZKKRQECS-UHFFFAOYSA-K 3-[18-(2-carboxyethyl)-8,13-bis(ethenyl)-3,7,12,17-tetramethylporphyrin-21,24-diid-2-yl]propanoic acid iron(3+) hydroxide Chemical compound [OH-].[Fe+3].[N-]1C2=C(C)C(CCC(O)=O)=C1C=C([N-]1)C(CCC(O)=O)=C(C)C1=CC(C(C)=C1C=C)=NC1=CC(C(C)=C1C=C)=NC1=C2 BMUDPLZKKRQECS-UHFFFAOYSA-K 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241000237536 Mytilus edulis Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000021051 daily weight gain Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 229940109738 hematin Drugs 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000002973 irritant agent Substances 0.000 description 1
- HPIGCVXMBGOWTF-UHFFFAOYSA-N isomaltol Natural products CC(=O)C=1OC=CC=1O HPIGCVXMBGOWTF-UHFFFAOYSA-N 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000020638 mussel Nutrition 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/0047—Air-conditioning, e.g. ventilation, of animal housings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/77—Liquid phase processes
- B01D53/78—Liquid phase processes with gas-liquid contact
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/40—Nitrogen compounds
- B01D2257/406—Ammonia
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2258/00—Sources of waste gases
- B01D2258/02—Other waste gases
- B01D2258/0266—Other waste gases from animal farms
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Genetics & Genomics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses an environment-friendly enzyme resistant to an alkaline environment of pig farm liquid dung and a preparation method thereof. The bacillus licheniformis obtained by the invention can grow and proliferate by taking the pig farm liquid dung as a main nutrient through on-site enrichment screening, and can obviously reduce ammonia nitrogen and total nitrogen. The invention takes the bacillus subtilis, the saccharomycetes, the lactic acid bacteria and the like as main materials and auxiliary materials to produce the environment-friendly enzyme, the product can be directly diluted by 10-30 times and then sprayed into a pigsty, and the environment-friendly enzyme has the effects of deodorization and ammonia gas reduction, improves the breeding environment and has the effect superior to similar products on the market.
Description
Technical Field
The invention relates to the technical field of environment-friendly enzymes, and particularly relates to an environment-friendly enzyme capable of resisting an alkaline environment of pig farm liquid dung and a preparation method thereof.
Background
Ammonia gas is a strong oneAn irritating gas. According to research reports, the average emission of ammonia gas of each fattening pig is 107.18-424.42mg/h, and the average emission of nitrogen per day of ten thousand pig farms that are slaughtered in one year reaches 105kg, wherein most of the nitrogen is ammonia gas (NH)3) The form is released to the atmosphere. The excessive concentration of ammonia gas in the piggery not only influences the growth and development of pigs, but also reduces the resistance of organisms, thereby inducing various diseases, causing serious economic loss to the production of the pigs, and simultaneously causing harm to feeders and the surrounding environment of a pig farm.
The pigs live in a low-concentration ammonia environment for a long time, generally do not produce obvious pathological reaction, but can cause the reduction of average daily feed intake, the reduction of the digestibility of feed and the reduction of the disease resistance. The ammonia concentration in the house is 25mg/m3When the piglet with the weight of 20kg grows, the growth speed is reduced by 5 percent; when the ammonia concentration rises to 50mg/m3In time, the growth rate of the piglets is reduced by 12 percent; the ammonia concentration is 100-150mg/m3The growth rate of piglets will be reduced by 30% at all times. The results of relevant studies on the influence of environmental conditions in the barn on the production performance show that as the ammonia concentration in the environment in the barn increases, the daily weight gain of pigs decreases, the feed-to-weight ratio increases as the ammonia concentration increases, and meanwhile, the higher ammonia concentration can also induce other diseases. After being sucked through a respiratory tract, ammonia gas in the pigsty enters blood through lung bubbles and is combined with hemoglobin, so that heme is changed into hematin, the oxygen carrying capacity of the hemoglobin and the oxidation performance of blood alkali storage and the heme are reduced, anemia and hypoxia appear, and the resistance of an organism to diseases is reduced. In addition, the ammonia gas in the pigsty can bring environmental protection problems, has strong pungent odor, influences the odor of surrounding air and influences the life of surrounding residents.
At present, the method for reducing ammonia gas mainly uses environment-friendly enzymes to degrade the ammonia gas. The environment-friendly ferment products used in the market mainly comprise bacillus, saccharomycetes, lactic acid bacteria, photosynthetic bacteria and the like, but no bacillus licheniformis products are used at present. At present, researches on the application of bacillus licheniformis in environment-friendly microbial agents are few, and domestic reports are basically not related.
Disclosure of Invention
The invention aims to provide an environment-friendly enzyme resistant to an alkaline environment of pig farm liquid manure and a preparation method thereof, through on-site enrichment screening, a bacillus licheniformis strain is obtained, the bacillus licheniformis strain can grow and proliferate by taking the pig farm liquid manure as a main nutrient and can obviously reduce ammonia nitrogen and total nitrogen, the bacillus licheniformis strain is taken as a main auxiliary material and bacillus subtilis, saccharomycetes, lactobacillus and the like are used for producing the environment-friendly enzyme, the product can be directly diluted by 10-30 times and then sprayed into a pig house, and the environment-friendly enzyme has deodorization and ammonia gas reduction effects, improves the breeding environment, has an effect superior to that of similar products on the market, and solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
an environment-friendly ferment resistant to an alkaline environment of pig farm liquid manure is prepared by inoculating bacillus licheniformis into a beef extract peptone culture medium for fermentation culture.
Further, the beef extract peptone medium comprises: 3g of beef extract, 10g of peptone, 5g of NaCl and 1000ml of distilled water, adjusting the pH value to 7.4-7.6, and sterilizing for 20min by high-pressure steam at 121 ℃.
The invention provides another technical scheme: a preparation method of an environment-friendly enzyme resistant to an alkaline environment of pig farm liquid manure comprises the steps of taking a bacillus licheniformis strain, inoculating the bacillus licheniformis strain into a beef extract peptone culture medium, carrying out shake culture at 37 ℃ for 12-18h to form a seed solution, carrying out large-tank fermentation for 24-48h, and adding fermented bacillus subtilis and lactic acid bacteria before subpackaging to obtain the environment-friendly enzyme.
Furthermore, the prepared environment-friendly ferment is rich in organic acid and peptide active substances, and the viable count of the ferment is 5.0-10.0x108cfu/ml。
Further, the prepared environment-friendly ferment can grow in the alkaline environment of liquid dung.
Compared with the prior art, the invention has the beneficial effects that:
according to the environment-friendly enzyme capable of resisting the alkaline environment of the pig farm liquid manure and the preparation method thereof, the bacillus licheniformis can grow and proliferate by taking the pig farm liquid manure as a main nutrient through on-site enrichment screening, and can obviously reduce ammonia nitrogen and total nitrogen. The bacillus subtilis, the saccharomycetes, the lactic acid bacteria and the like are taken as main materials and auxiliary materials to produce the environment-friendly enzyme, the product can be directly diluted by 10-30 times and then sprayed into a pigsty, the deodorization and ammonia gas reduction effects are achieved, the breeding environment is improved, and the effect is superior to that of similar products on the market.
Detailed Description
The following examples will explain the present invention in detail, however, the present invention is not limited thereto. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention provides an environment-friendly ferment resistant to an alkaline environment of pig farm liquid dung, which is prepared by inoculating bacillus licheniformis into a beef extract peptone culture medium for fermentation culture.
Wherein, the beef extract peptone culture medium comprises: 3g of beef extract, 10g of peptone, 5g of NaCl and 1000ml of distilled water, adjusting the pH value to 7.4-7.6, and sterilizing for 20min by high-pressure steam at 121 ℃.
In order to better explain the invention, the invention also provides a preparation method of the environment-friendly enzyme resistant to the alkaline environment of the pig farm liquid manure, which comprises the steps of taking a bacillus licheniformis strain, inoculating the bacillus licheniformis strain into a beef extract peptone culture medium, carrying out shake culture at 37 ℃ for 12-18h to form a seed solution, carrying out large-tank fermentation for 24-48h, and adding the fermented bacillus subtilis and lactic acid bacteria before subpackaging to obtain the environment-friendly enzyme.
In the above embodiment, the prepared enzyme is rich in organic acids and peptides, and has viable count of 5.0-10.0x108cfu/ml。
In the embodiment, the prepared environment-friendly enzyme can grow in an alkaline environment of liquid dung, the concentration of ammonia is obviously reduced, the culture environment is improved, and the effect is superior to that of similar products in the market.
In order to further verify the functionality of the environmental-friendly ferment of the invention, the following specific embodiments are also provided:
example 1:
purpose and meaning: the influence of the environment-friendly enzyme disclosed by the invention on the concentration of the ammonia gas in the pigsty is investigated.
The test site comprises: hanshi Wei Jiang pig farm with mussels and Wei Zhuang.
2 piggeries in total, and 1-40 columns in the No. 1 piggery, which are experimental groups; column 41-80 in colony house 2, control group; diluting 40kg of environment-friendly enzyme by 10 times, and uniformly spraying the environment-friendly enzyme on the experimental group floor; the control group was not treated; during the experiment, the doors, windows and fans of 2 houses are all closed to achieve the purpose of separation; the ammonia gas measuring point is set at the center of the corresponding colony house, 1.2m away from the ground, and a Hima ammonia gas detector is used for detecting.
The results of the experiment are as follows:
from the above table, it can be seen that: compared with a control group, the ammonia concentration of the experimental group is obviously reduced, and the ammonia reduction effect of the environment-friendly ferment is shown.
Example 2:
purpose and meaning: the influence of the environment-friendly enzyme on the ammonia concentration of the environment-friendly sewage of the pig farm is inspected.
The test site comprises: the Hanshi Weihua pig farm is an environment-friendly area.
The control group is the average number of 10 detections in the normal process, the experimental group adds 0.5% of the addition amount of the environment-friendly enzyme lactobacillus into the anaerobic reactor, the sewage is fermented in the anaerobic reactor for one week, and the ammonia nitrogen and COD content in the anaerobic sedimentation tank is detected; according to a normal flow, when sewage enters two levels of A/O tanks, bacillus is added according to the addition amount of 0.5 percent, aeration is carried out for 6-7 days, and ammonia nitrogen and COD are detected.
The results of the experiment are as follows:
from the above table, it can be seen that: compared with a control group, the concentrations of ammonia nitrogen and COD in the experimental group are obviously reduced, and the effect of the environment-friendly enzyme is shown.
In summary, the following steps: according to the environment-friendly enzyme capable of resisting the alkaline environment of the pig farm liquid manure and the preparation method thereof, the bacillus licheniformis can grow and proliferate by taking the pig farm liquid manure as a main nutrient through on-site enrichment screening, and can obviously reduce ammonia nitrogen and total nitrogen. The bacillus subtilis, the saccharomycetes, the lactic acid bacteria and the like are taken as main materials and auxiliary materials to produce the environment-friendly enzyme, the product can be directly diluted by 10-30 times and then sprayed into a pigsty, the deodorization and ammonia gas reduction effects are achieved, the breeding environment is improved, and the effect is superior to that of similar products on the market.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (5)
1. An environment-friendly ferment resistant to an alkaline environment of pig farm liquid manure is characterized in that the ferment is prepared by inoculating bacillus licheniformis into a beef extract peptone culture medium for fermentation culture.
2. The environmentally friendly ferment of claim 1, wherein the beef extract peptone medium comprises: 3g of beef extract, 10g of peptone, 5g of NaCl and 1000ml of distilled water, adjusting the pH value to 7.4-7.6, and sterilizing for 20min by high-pressure steam at 121 ℃.
3. The preparation method of the environment-friendly ferment resistant to the alkaline environment of the pig farm manure as claimed in claim 1, wherein a bacillus licheniformis strain is taken and inoculated in a beef extract peptone medium, shaking culture is carried out at 37 ℃ for 12-18h to form a seed solution, then large tank fermentation is carried out for 24-48h, and fermented bacillus subtilis and lactic acid bacteria are added before subpackaging to obtain the environment-friendly ferment.
4. The method for preparing the environmental-friendly ferment resisting the alkaline environment of the pig farm manure as claimed in claim 3, wherein the prepared environmental-friendly ferment is rich in organic acid and peptide active substances,the viable count is 5.0-10.0x108cfu/ml。
5. The method of claim 3, wherein the prepared green ferment can grow in the alkaline environment of the liquid manure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011155223.7A CN112322522A (en) | 2020-10-26 | 2020-10-26 | Environment-friendly enzyme resistant to alkaline environment of pig farm liquid dung and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011155223.7A CN112322522A (en) | 2020-10-26 | 2020-10-26 | Environment-friendly enzyme resistant to alkaline environment of pig farm liquid dung and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112322522A true CN112322522A (en) | 2021-02-05 |
Family
ID=74311739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011155223.7A Pending CN112322522A (en) | 2020-10-26 | 2020-10-26 | Environment-friendly enzyme resistant to alkaline environment of pig farm liquid dung and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112322522A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408569A (en) * | 2019-08-12 | 2019-11-05 | 安徽天邦生物技术有限公司 | Utilize the environmental protection ferment and preparation method thereof of class bacillus licheniformis BP17 production |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009209222A (en) * | 2008-03-03 | 2009-09-17 | Iichiro Yamakawa | Manufacturing method and usage of enzyme nutritive solution, and organic resource circulation method |
| CN110408569A (en) * | 2019-08-12 | 2019-11-05 | 安徽天邦生物技术有限公司 | Utilize the environmental protection ferment and preparation method thereof of class bacillus licheniformis BP17 production |
-
2020
- 2020-10-26 CN CN202011155223.7A patent/CN112322522A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009209222A (en) * | 2008-03-03 | 2009-09-17 | Iichiro Yamakawa | Manufacturing method and usage of enzyme nutritive solution, and organic resource circulation method |
| CN110408569A (en) * | 2019-08-12 | 2019-11-05 | 安徽天邦生物技术有限公司 | Utilize the environmental protection ferment and preparation method thereof of class bacillus licheniformis BP17 production |
Non-Patent Citations (3)
| Title |
|---|
| S. CHRIS FELSHIA 等: ""Elucidation of 2, 4-Dichlorophenol degradation by Bacillus licheniformis strain SL10"", 《ENVIRONMENTAL TECHNOLOGY》 * |
| 南京农业大学自然资源与环境科学系等: "《农业持续发展的资源环境研究与管理》", 31 October 1994, 中国农业科技出版社 * |
| 印遇龙等: "《改革开放40年中国猪业发展与进步 猪场废弃物处理和资源化利用》", 31 October 2018, 中国农业大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408569A (en) * | 2019-08-12 | 2019-11-05 | 安徽天邦生物技术有限公司 | Utilize the environmental protection ferment and preparation method thereof of class bacillus licheniformis BP17 production |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103875893B (en) | Many bacterial classifications composite microbial feed additive and preparation method thereof | |
| CN105002116B (en) | A kind of microbial straw feed microbial inoculum and preparation method thereof | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN109607818A (en) | A kind of microorganism improver of water quality and preparation method thereof | |
| CN103088010A (en) | Biological health padding and preparation method thereof | |
| CN102286376A (en) | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof | |
| CN107568071A (en) | Biological deodorant for livestock and poultry farm | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN110129235B (en) | Heat-resistant lactobacillus plantarum and application thereof in agricultural production | |
| CN108913626B (en) | Livestock and poultry manure deodorizing composite microbial inoculum and preparation method thereof | |
| CN107801837A (en) | A kind of fodder utilizes the bio-fermentation process of chicken manure | |
| CN107176891A (en) | It is a kind of to promote the biological agent and its production technology of stalk fast degradation | |
| CN110257292A (en) | A kind of microbial bacterial agent used for aquiculture and preparation method thereof | |
| CN106854627A (en) | A kind of composite bacteria agent for promoting the flat angle stratiomyiid larval growth of speck and application | |
| CN101836688B (en) | Preparation method of antibiotic-free microbial fermentation feed | |
| CN113215051A (en) | Method for preparing feed probiotics by using lactobacillus through rice flour wastewater and passion fruit peel | |
| CN109666597A (en) | A kind of complex microorganism inorganic agent and preparation method thereof of fermentation ensiling Huang storage feed | |
| CN112322522A (en) | Environment-friendly enzyme resistant to alkaline environment of pig farm liquid dung and preparation method thereof | |
| CN107760612A (en) | A kind of aspergillus niger yy07 bacterial strains and its application in solid fermentation produces feeding acid protease | |
| CN103497901A (en) | Novel composite probiotic preparation for pig-raising fermentation bed | |
| CN109627064A (en) | Straw manures preparation method for material and straw organic fertilizer | |
| CN101411319B (en) | Ecological method for feeding pigs with low discharging | |
| CN107955798A (en) | A kind of industrial solid castoff Feedstock treating fermenting microbial inoculum | |
| CN102994427A (en) | Bacillus mucilaginosus and preparation method of crude polysaccharide of bacillus mucilaginosus beneficial to growth of poultry | |
| CN110408569A (en) | Utilize the environmental protection ferment and preparation method thereof of class bacillus licheniformis BP17 production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210205 |
|
| RJ01 | Rejection of invention patent application after publication |