CN102746999A - LH microbes and their preparation method and use - Google Patents

LH microbes and their preparation method and use Download PDF

Info

Publication number
CN102746999A
CN102746999A CN2011100977713A CN201110097771A CN102746999A CN 102746999 A CN102746999 A CN 102746999A CN 2011100977713 A CN2011100977713 A CN 2011100977713A CN 201110097771 A CN201110097771 A CN 201110097771A CN 102746999 A CN102746999 A CN 102746999A
Authority
CN
China
Prior art keywords
bacteria
preparation
mikrobe
bacillus
bacterial classification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011100977713A
Other languages
Chinese (zh)
Inventor
张金松
王全喜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN2011100977713A priority Critical patent/CN102746999A/en
Publication of CN102746999A publication Critical patent/CN102746999A/en
Pending legal-status Critical Current

Links

Landscapes

  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Sludge (AREA)

Abstract

The invention relates to LH microbes and their preparation method and use. The LH microbes are novel microbes which can effective treat sewage and reduce a discharge amount of sludge. The invention provides the preparation method and the use of the LH microbes. The LH microbes are combined multi-bacterium microbes. The combined multi-bacterium microbes comprise bacillus natto, bifidobacteria, bacilli, actinomycetes, photosynthetic bacteria, acetic acid bacteria, saccharomycetes, lactic acid bacteria and nitrifying bacteria.

Description

A kind of LH mikrobe, preparation method and application thereof
Technical field
The present invention relates to microbial technology field, specifically, the present invention relates to a kind of LH mikrobe, preparation method and application thereof.
Background technology
Along with the continuous progress of national economy and social development, the consequence that people bring serious day by day ecological environmental pollution has given increasing attention, and some river and lake foul because of the sewage discharge blackening of a large amount of lives, agricultural, industry, catering trade; The window that contiguous resident has all dare not be opened, and especially in summer, walks by the river and can both smell the stink that assails the nostrils; The municipal sewage plant though purified millions of ton sewage every day, has also produced the organic sludge of number with kiloton simultaneously; These not only black not only smelly but also malicious mud also can only be handled with the mode of transhipment landfill in way far away at present; Also often there is sewage work to steal the mud of falling the foul sewage on newspaper and the network and stops up the report that the river course causes a large amount of fish kills, large department store, star hotel; The underground sewage in large-scale restaurant holds the foul smell that the pond gives out; At present be that large, medium and small city management is difficult to resolve the headache problem of one of determining most, serious environmental is polluted the living and studying that has had influence on people, and an urgent demand government department carries out governance and protection to environment; Present administration way falls behind and is costly, often all is the old tradition mode of curing the symptoms, not the disease.The Along with people's growth in the living standard, people are also increasingly high to the demand of ecological nonstaple food, and people are able to eat free of contamination fishery products of clean health and poultry nonstaple food, are determining the healthy and long-lived of human body.
Summary of the invention
The purpose of this invention is to provide a kind of novel LH mikrobe, can effectively dispose of sewage, reduce the mud discharge capacity.
Another object of the present invention provides above-mentioned LH microorganism preparation method.
Another object of the present invention provides above-mentioned LH application of microorganism.
For achieving the above object; The invention provides a kind of LH mikrobe; Said LH mikrobe comprises Bacillus natto, bifidus bacillus, genus bacillus, actinomycetes, photosynthetic bacteria, acetic bacteria, purple thiobacterium, green sulphur bacteria, yeast, milk-acid bacteria and nitrifier for many bacterial classifications of associating mikrobe in said many bacterial classifications of associating mikrobe.
Wherein, the various viable bacteria total contents of said LH mikrobe are no less than 10,000,000,000/ml.
The present invention also provides a kind of LH microorganism preparation method; This preparation method is: bacterial classification stoste and fermented material substratum are put into fermentor tank, and at 25-38 ℃ of bottom fermentation 5-8 days, recording pH value was 3.5-8; When sending sour-sweet vinosity, make said LH mikrobe; Comprise in the said bacterial classification stoste: Bacillus natto, bifidus bacillus, genus bacillus, actinomycetes, photosynthetic bacteria, acetic bacteria, yeast, milk-acid bacteria, purple thiobacterium, green sulphur bacteria and nitrifier.
Wherein, the weight part of each component is in the said bacterial classification stoste:
Bacillus natto: 3-6% bifidus bacillus: 14% genus bacillus: 9-10%
Actinomycetes: 15-16% photosynthetic bacteria: 11-13% acetic bacteria: 6%
Yeast: purple thiobacterium: the 5-6% of 5-6% milk-acid bacteria: 6-7%
Green sulphur bacteria: 5-6% nitrifier: 13-16%
Further; Comprise in the said fermented material substratum: sugar, vitamins C, potassium primary phosphate, peptide urea and water, the weight quota of said each component is respectively: 1000 parts of bacterial classification stoste 2-6 parts, sugared 1-9 part, vitamins C 0.05-0.15 part, potassium primary phosphate 0.2-0.5 part, peptide urea 0.3-1 part, water.
Wherein, said sugar is one or more in brown sugar, honey or the ponding (sugar refinery's tankage).
Further, also comprise in fish slurry, light calcium carbonate, agar, peptone, salt or the rock salt one or more in the said fermented material substratum.
As another preferred version; Comprise in the substratum of fermented material described in the present invention: sugar, vitamins C, peptone, salt and water, the weight quota of said each component is respectively: bacterial classification stoste 2-6 part, sugared 1-9 part, vitamins C 0.05-0.15 part, 3 parts of peptones, salt 0.1-0.5 part, purify waste water 1000 parts.
The prepared LH mikrobe of the inventive method can be used for sewage treatment area.
Adopt LH microbiological treatment of the present invention to deceive smelly river regulation, need not change water, do not need desilting, it is black smelly to eliminate the river course, and effect is got instant result, and maintenance time is long, not pollutent river course once more; In the sludge of sewage treatment plant decrement process, need not change equipment, drop into LH mikrobe of the present invention, the sludge of sewage treatment plants discharge capacity is significantly reduced, LH mikrobe of the present invention can make a large amount of mud discharging decrements of sewage work become a reality.Can prevent that with LH mikrobe new technology breed poultry and aquatic products fish and shrimp Animal diseases from increasing output; Make the pig farm; The poulty house is destitute of smell, and makes plant-animal more delicious, makes citizen can be able to eat not contaminated healthy agricultural byproducts; Solve the breed at family, vast rural culture and the problem of sale, effectively supported the vegetable basket of urban market.
LH microorganism preparation method of the present invention has adopted new fermentation process, has increased the effect of fermentation greatly, has practiced thrift a large amount of electric energy; Adopted complex microorganism harmless in the Nature, made it can reduce cost of manufacture; Be fit to the present national conditions of China; The pollutent of the black smelly river and lake of effectively degrading and breed water water body is removed the peculiar smell in the water, has guaranteed the water quality of river course and aquaculture water; The a large amount of black foul sewage mud that the municipal sewage plant is produced effectively reduces, and effects of energy saving and emission reduction is remarkable.
Prepare that each component can both can buy at home in the fermented material substratum that LH of the present invention Institute of Micro-biology adopts, preparation is convenient economical.
Embodiment
Below through embodiment the present invention is described further, but be not limited thereto.
Embodiment 1
Get 1000 kilograms of pure water, 3 kilograms of LH bacterial classification stostes, 1 kilogram in honey, 6 kilograms in ponding; 3 kilograms of peptones, 2 kilograms in fish slurry, 0.1 kilogram of salt; Vitamins C 50 grams add in the mash-back, and the cover that will ferment after stirring covers and makes its temperature remain on 25-38 ℃; Stir once every day, each three minutes, stirs the back stop and cover cover again after 5 minutes; The 5th day or the 6th day its pH value reach more than 4 and can smell and send the mashed sour-sweet peculiar smell of sugarcane and promptly process, and it is enclosed within preserves in the lighttight plastic containers or directly use.Used bacterial classification stoste comprises Bacillus natto 6%, bifidus bacillus 14%, genus bacillus 9%, actinomycetes 15%, photosynthetic bacteria 11%, acetic bacteria 6%, yeast 6%, milk-acid bacteria 7% and nitrifier 16%, purple thiobacterium 5%, green sulphur bacteria 5% in the present embodiment.Total colony count viable bacteria content in cultivating the every 1ml in back by fermentation can reach 3 * 10 8-2.8 * 10 9Between individual, has good deodorizing and to the sludge degradation function.
In the mud decrement engineering of municipal sewage plant, need not add any equipment; Being used for the sludge from wastewater treatment plant decrement discharge handles; Can effectively reduce the mud amount of sending reaches more than the 50%-70%; Handle the back change of water quality, it is as clear as crystal to reach national water treatment emission standard water quality, make original dissolved oxygen be zero black smelly river in a short time dissolved oxygen rise to 4-6.5.
Embodiment 2
Get in 4 kilograms of LH bacterial classification stostes and purify waste water 1000 kilograms, 8 kilograms in brown sugar, vitamins C 100 grams, potassium primary phosphate 200 grams, 0.3 kilogram of adding fermentor tank of peptide urea; The cover that will ferment after stirring covers and makes its temperature remain on 30-36 ℃ of bottom fermentation eight days; PH value is 8 and smell and have sour-sweet vinosity promptly to process, and it is enclosed within preserves in the lighttight plastic containers or directly use.Comprise Bacillus natto 5%, bifidus bacillus 14%, genus bacillus 10%, actinomycetes 16%, photosynthetic bacteria 13%, acetic bacteria 6%, yeast 5%, milk-acid bacteria 6% and nitrifier 13%, purple thiobacterium 6%, green sulphur bacteria 6% in the present embodiment in the bacterial classification stoste.Total colony count viable bacteria content of every 1ml can reach 3 * 10 after the fermentative prodn 8-2.8 * 10 9Between individual.Be used for high-density raising fish and shrimp industry, raise pigs, raise chickens the advantage that has other breeding way not replace; Be used for black smelly river course water, make original dissolved oxygen almost nil, fishes and shrimps disappearance in the river, not only black but also smelly river is removed stink in a short time rapidly, and dissolved oxygen rises to 4-6.5, and the ammonia nitrogen index reduces rapidly, and it is clear that river becomes, and can cultured fishes.
Embodiment 3
Get 1000 kilograms of pure water, 3 kilograms of LH bacterial classification stostes, 1 kilogram in honey, 6 kilograms in ponding; 3 kilograms of peptones, 2 kilograms in fish slurry, 0.1 kilogram of salt; Vitamins C 50 grams add in the mash-back, and the cover that will ferment after stirring covers and makes its temperature remain on 30-36 ℃; Stir once every day, each three minutes, stirs the back stop and cover cover again after 5 minutes; The 5th day or the 6th day its pH value reach 4.5 and can smell and send the mashed sour-sweet peculiar smell of sugarcane and promptly process, and it is enclosed within preserves in the lighttight plastic containers or directly use.Used bacterial classification stoste comprises Bacillus natto 6%, bifidus bacillus 14%, genus bacillus 9%, actinomycetes 15%, photosynthetic bacteria 11%, acetic bacteria 6%, yeast 6%, milk-acid bacteria 7% and nitrifier 16%, purple thiobacterium 5%, green sulphur bacteria 5% in the present embodiment.Total colony count viable bacteria content in cultivating the every 1ml in back by fermentation can reach 3 * 10 8-2.8 * 10 9Between individual, has good deodorizing and to the sludge degradation function.
In the mud decrement engineering of municipal sewage plant, need not add any equipment; Being used for the sludge from wastewater treatment plant decrement discharge handles; Can effectively reduce the mud amount of sending reaches more than the 50%-70%; Handle the back change of water quality, it is as clear as crystal to reach national water treatment emission standard water quality, make original dissolved oxygen be zero black smelly river in a short time dissolved oxygen rise to 4-6.5.

Claims (9)

1. LH mikrobe; It is characterized in that: said LH mikrobe comprises Bacillus natto, bifidus bacillus, genus bacillus, actinomycetes, photosynthetic bacteria, acetic bacteria, purple thiobacterium, green sulphur bacteria, yeast, milk-acid bacteria and nitrifier for many bacterial classifications of associating mikrobe in said many bacterial classifications of associating mikrobe.
2. LH mikrobe as claimed in claim 1 is characterized in that: the various viable bacteria total contents of said LH mikrobe are no less than 10,000,000,000/ml.
3. LH microorganism preparation method, it is characterized in that this preparation method is: bacterial classification stoste and fermented material substratum are put into fermentor tank, and at 25-38 ℃ of bottom fermentation 5-8 days, recording pH value was 3.5-8, when sending sour-sweet vinosity, made said LH mikrobe; Comprise in the said bacterial classification stoste: Bacillus natto, bifidus bacillus, genus bacillus, actinomycetes, photosynthetic bacteria, acetic bacteria, yeast, milk-acid bacteria, purple thiobacterium, green sulphur bacteria and nitrifier.
4. preparation method as claimed in claim 3 is characterized in that: the weight part of each component is in the said bacterial classification stoste:
Bacillus natto: 3-6% bifidus bacillus: 14% genus bacillus: 9-10%
Actinomycetes: 15-16% photosynthetic bacteria: 11-13% acetic bacteria: 6%
Yeast: purple thiobacterium: the 5-6% of 5-6% milk-acid bacteria: 6-7%
Green sulphur bacteria: 5-6% nitrifier: 13-16%
5. preparation method as claimed in claim 3; It is characterized in that: comprise in the said fermented material substratum: sugar, vitamins C, potassium primary phosphate, peptide urea and water, the weight quota of said each component is respectively: 1000 parts of bacterial classification stoste 2-6 parts, sugared 1-9 part, vitamins C 0.05-0.15 part, potassium primary phosphate 0.2-0.5 part, peptide urea 0.3-1 part, water.
6. preparation method as claimed in claim 5 is characterized in that: said sugar is one or more in brown sugar, honey or the ponding (sugar refinery's tankage).
7. preparation method as claimed in claim 5 is characterized in that: also comprise in fish slurry, light calcium carbonate, agar, peptone, salt or the rock salt one or more in the said fermented material substratum.
8. preparation method as claimed in claim 3; It is characterized in that: comprise in the said fermented material substratum: sugar, vitamins C, peptone, salt and water, the weight quota of said each component is respectively: bacterial classification stoste 2-6 part, sugared 1-9 part, vitamins C 0.05-0.15 part, 3 parts of peptones, salt 0.1-0.5 part, purify waste water 1000 parts.
9. the LH mikrobe that preparation method as claimed in claim 3 obtains is in the application of sewage treatment area.
CN2011100977713A 2011-04-19 2011-04-19 LH microbes and their preparation method and use Pending CN102746999A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011100977713A CN102746999A (en) 2011-04-19 2011-04-19 LH microbes and their preparation method and use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011100977713A CN102746999A (en) 2011-04-19 2011-04-19 LH microbes and their preparation method and use

Publications (1)

Publication Number Publication Date
CN102746999A true CN102746999A (en) 2012-10-24

Family

ID=47027495

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011100977713A Pending CN102746999A (en) 2011-04-19 2011-04-19 LH microbes and their preparation method and use

Country Status (1)

Country Link
CN (1) CN102746999A (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103468608A (en) * 2013-08-27 2013-12-25 重庆瑞宝环保工程有限公司 Complex flora for sewage treatment and culture method of complex flora
CN103880194A (en) * 2014-03-20 2014-06-25 广西大学 Microorganism water purification agent and preparation method thereof
CN104120085A (en) * 2013-04-24 2014-10-29 刘智云 Composite microbe deodorizing and degrading inoculant, and preparation method and application thereof
CN105399218A (en) * 2015-12-18 2016-03-16 仝荣 Sewage treatment method of biological bacteria
CN105399213A (en) * 2015-12-12 2016-03-16 常州大学 Microbial degradation method for phenol-containing waste water
CN105441327A (en) * 2015-12-27 2016-03-30 陈景河 Hydrolyzed fish soluble microbial culture medium
CN105536508A (en) * 2015-12-22 2016-05-04 湖南普泰尔微科环保科技有限公司 Deodorization method by using microbial deodorant cooperating with high-pressure spray machine
CN105536507A (en) * 2015-12-22 2016-05-04 湖南普泰尔微科环保科技有限公司 Washing deodorization tower and deodorization method by using washing deodorization tower cooperating with microbial deodorant
CN110382721A (en) * 2017-01-13 2019-10-25 Scd益生菌持续社区发展有限责任公司 Biodegradable composition and/or probiotics biochemicals and its application method during leather tanning
CN110438031A (en) * 2019-06-28 2019-11-12 中南大学 A kind of microbial deoderizer and preparation method thereof

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1462728A (en) * 2003-05-30 2003-12-24 成都海发(集团)股份有限公司 Technique for treating wastewater by using microorganism
CN1718546A (en) * 2004-07-08 2006-01-11 李新生 Device for treating and utilizing domestic wastewater in public places
CN1952107A (en) * 2006-11-16 2007-04-25 任宪君 Natto slime hypha and its preparing method and application
CN101037655A (en) * 2006-11-29 2007-09-19 金明记 High-effective microorganism complex agent and method of using the same for treating city sewage
CN101219844A (en) * 2008-01-23 2008-07-16 太原理工大学 Technique for biological treatment of mine acidic wastewater
CN101323488A (en) * 2007-06-12 2008-12-17 同济大学 Method for improving sewerage dephosphorization and denitrification effect
CN101503664A (en) * 2009-03-12 2009-08-12 沈阳惠泽生物制品有限公司 Method for producing efficient composite microbial preparation by livestock and poultry liquid dung
CN101591069A (en) * 2009-05-21 2009-12-02 东莞圣源环保科技有限公司 The treatment process of polluted lake water body
CN101600762A (en) * 2007-02-05 2009-12-09 株式会社日本触媒 Granular water absorber and manufacture method thereof

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1462728A (en) * 2003-05-30 2003-12-24 成都海发(集团)股份有限公司 Technique for treating wastewater by using microorganism
CN1718546A (en) * 2004-07-08 2006-01-11 李新生 Device for treating and utilizing domestic wastewater in public places
CN1952107A (en) * 2006-11-16 2007-04-25 任宪君 Natto slime hypha and its preparing method and application
CN101037655A (en) * 2006-11-29 2007-09-19 金明记 High-effective microorganism complex agent and method of using the same for treating city sewage
CN101600762A (en) * 2007-02-05 2009-12-09 株式会社日本触媒 Granular water absorber and manufacture method thereof
CN101323488A (en) * 2007-06-12 2008-12-17 同济大学 Method for improving sewerage dephosphorization and denitrification effect
CN101219844A (en) * 2008-01-23 2008-07-16 太原理工大学 Technique for biological treatment of mine acidic wastewater
CN101503664A (en) * 2009-03-12 2009-08-12 沈阳惠泽生物制品有限公司 Method for producing efficient composite microbial preparation by livestock and poultry liquid dung
CN101591069A (en) * 2009-05-21 2009-12-02 东莞圣源环保科技有限公司 The treatment process of polluted lake water body

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
苏本森: "微生物在污水处理中的应用", 《山东环境》 *
郭丹钊 等: "一株内生真菌的原生质体制备及再生过程研究", 《河南师范大学学报(自然科学版)》 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104120085A (en) * 2013-04-24 2014-10-29 刘智云 Composite microbe deodorizing and degrading inoculant, and preparation method and application thereof
CN103468608A (en) * 2013-08-27 2013-12-25 重庆瑞宝环保工程有限公司 Complex flora for sewage treatment and culture method of complex flora
CN103880194A (en) * 2014-03-20 2014-06-25 广西大学 Microorganism water purification agent and preparation method thereof
CN103880194B (en) * 2014-03-20 2016-08-17 广西大学 The preparation technology of a kind of microbiological water purification agent and the use of this water purification microbial inoculum
CN105399213A (en) * 2015-12-12 2016-03-16 常州大学 Microbial degradation method for phenol-containing waste water
CN105399218A (en) * 2015-12-18 2016-03-16 仝荣 Sewage treatment method of biological bacteria
CN105536508A (en) * 2015-12-22 2016-05-04 湖南普泰尔微科环保科技有限公司 Deodorization method by using microbial deodorant cooperating with high-pressure spray machine
CN105536507A (en) * 2015-12-22 2016-05-04 湖南普泰尔微科环保科技有限公司 Washing deodorization tower and deodorization method by using washing deodorization tower cooperating with microbial deodorant
CN105536508B (en) * 2015-12-22 2018-06-29 湖南普泰尔环境股份有限公司 A kind of deodorizing method of microbial deodorant cooperation high pressure spraying machine
CN105536507B (en) * 2015-12-22 2018-08-21 湖南普泰尔环境股份有限公司 A kind of deodorizing method for washing deodorizing tower and its coordinating microbial deodorant
CN105441327A (en) * 2015-12-27 2016-03-30 陈景河 Hydrolyzed fish soluble microbial culture medium
CN110382721A (en) * 2017-01-13 2019-10-25 Scd益生菌持续社区发展有限责任公司 Biodegradable composition and/or probiotics biochemicals and its application method during leather tanning
CN110438031A (en) * 2019-06-28 2019-11-12 中南大学 A kind of microbial deoderizer and preparation method thereof

Similar Documents

Publication Publication Date Title
CN102746999A (en) LH microbes and their preparation method and use
Zhou et al. Role and functions of beneficial microorganisms in sustainable aquaculture
CN101760431B (en) A kind of compound microbial culture starter and application thereof
Chuntapa et al. Water quality control using Spirulina platensis in shrimp culture tanks
CN101074144B (en) Ecological treatment and recovering utilization for farm
CN102286376B (en) Microbial inoculum for high-efficiency fermenting bed and preparation method thereof
CN103937695B (en) A kind of composite bacteria agent and manufacture method thereof of processing livestock and poultry cultivation sewage
CN101356950B (en) Preparation method of composite bacteria fermentation bed
CN101082028A (en) Preparation of water-adjusting bacterium and method for restoring aquaculture environment
CN105132307B (en) It is a kind of applied to the composite bacillus microbial water-purifying agent of ornamental fish and its application
CN108793587B (en) Rapid treatment method for pig farm urine-soaked manure waste liquid
CN105886440A (en) Bacillus licheniformis and microbial agent and application and method of microbial agent in treatment of agricultural wastes
CN101921710B (en) Repairing agent for microbes in water bodies of excessive culture zones
CN100480373C (en) Microbe for degrading nitrite, separating and bring up method, and application
CN102899277B (en) Bacillus cereus and application thereof
CN104787899A (en) Bacterial inhibition type water purifier for marine aquaculture and preparation method thereof
CN106854627A (en) A kind of composite bacteria agent for promoting the flat angle stratiomyiid larval growth of speck and application
Kasan et al. Application of biofloc technology (BFT) in shrimp aquaculture industry
CN105754903A (en) Method for cultivating photosynthetic bacteria in Rhodopseudomonas on large scale
CN105600942A (en) Method for formation of bioflocs by cyanobacterial bloom
CN100556828C (en) The biological treating method of eutrophication water
CN110054302A (en) Inhibit the processing material and preparation method thereof of algal grown
CN103145460A (en) Bio-organic fertilizer for preventing disease for aquaculture and preparation method thereof
CN101082033A (en) Preparation of living-benefit bacterium and method for restoring aquaculture environment
CN102726634A (en) Microecology feed for sea cucumbers, and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20121024