CN110813077A - Environment-friendly deodorant enzyme, and preparation method and application thereof - Google Patents
Environment-friendly deodorant enzyme, and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an environment-friendly deodorant enzyme, a preparation method and application thereof, wherein the preparation method comprises the following steps: adding natural plants, strains and nutrient substances into a fermentation tank, adding drinking water, sealing, and activating in a shade place at 15-40 ℃ for 1-15 days to obtain a bacterial liquid; and filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme. The environment-friendly deodorant enzyme prepared by the preparation method disclosed by the invention has the effects of improving the concentration of the environment-friendly deodorant enzyme, enabling the effective dilution multiple to reach 1000 times, greatly reducing the using amount in a large range, reducing the cost on transportation and storage and finally greatly improving the cost performance of the product through the physiological metabolism of microbial strains and natural plant enzymes.
Description
Technical Field
The invention relates to the field of preparation of microbial preparations, and in particular relates to an environment-friendly deodorant enzyme, and a preparation method and application thereof.
Background
In recent years, the enzyme and the preparation product thereof are increasingly widely applied in the fields of planting, breeding, environmental protection, health care and the like. The production of the enzyme deodorant reduces the air pollution problem of odor source odor areas such as garbage disposal plants, garbage transfer stations, garbage landfills, sewage disposal plants, farms, public toilets, toilets and the like, improves the life quality of people living in the odor problem range, and brings great contribution to the ecological environmental protection industry. However, the biological deodorant in the current market is generally higher in price, lower in concentration and larger in dosage, and brings inconvenience to users.
Disclosure of Invention
Based on the above problems, the present invention aims to provide a method for preparing an environment-friendly deodorant enzyme.
Another objective of the present invention is to provide an environment-friendly deodorant enzyme.
The invention further aims to provide application of the environment-friendly deodorant enzyme in preparation of deodorants for garbage disposal sites, garbage transfer stations, garbage burial sites, sewage disposal sites, breeding farms and toilets.
The technical scheme of the invention is as follows:
a preparation method of an environment-friendly deodorant enzyme comprises the following steps:
adding natural plants, strains and nutrient substances into a fermentation tank, adding drinking water, sealing, and activating in a shade place at 15-40 ℃ for 1-15 days to obtain a bacterial liquid; wherein the natural plant comprises one or more of herba Menthae, lignum Cinnamomi Camphorae, YOUJIELI tree and tea tree; the strain comprises more than two of lactobacillus, lactobacillus plantarum, bacillus subtilis and saccharomycetes; the nutrient substances comprise more than two of glucose, lactose, brown sugar, inorganic salt, starch, beef extract, yeast powder, yeast extract, peptone, agar, water, citric acid, lactic acid, acetic acid, sodium hydroxide, potassium hydroxide and phosphate buffer salt;
and filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme.
In one embodiment, in the preparation method of the environmentally friendly deodorant enzyme, the weight ratio of the natural plant to the nutrient is 1: 10-1: 100000; preferably, the weight ratio of the natural plants to the nutrient substances is 1: 5-1: 50000.
In one embodiment, in the preparation method of the environment-friendly deodorant enzyme, the ratio of the strain to the nutrient is 1:10 to 1: 100000; preferably, the weight ratio of the strain to the nutrient is 1: 5-1: 50000. In one embodiment, in the preparation method of the environment-friendly deodorant enzyme, the amount of the drinking water is 1 to 1000 times of the total weight of the natural plants, the strains and the nutrients; preferably, the adding amount of the drinking water is 2-500 times of the total weight of the natural plants, the strains and the nutrient substances.
In one embodiment, in the preparation method of the environment-friendly deodorant enzyme, the activation temperature is 20-35 ℃, and the activation time is 5-10 days.
The invention also provides the environment-friendly deodorant enzyme prepared by the preparation method.
The invention further provides application of the environment-friendly deodorant enzyme in preparation of deodorants for garbage disposal sites, garbage transfer stations, garbage landfills, sewage disposal sites, farms and toilets.
The environment-friendly deodorant enzyme prepared by the preparation method disclosed by the invention has the effects of improving the concentration of the environment-friendly deodorant enzyme, enabling the effective dilution multiple to reach 1000 times, greatly reducing the using amount in a large range, reducing the cost on transportation and storage and finally greatly improving the cost performance of the product through the physiological metabolism of microbial strains and natural plant enzymes.
Drawings
FIG. 1 is a flow chart of a process for preparing an environmentally friendly deodorant enzyme according to an embodiment of the present invention.
Detailed Description
The preferred embodiments of the present invention will be described in further detail with reference to the accompanying drawings.
In order to overcome the defects of low concentration, large using amount, high use cost and the like of the biological deodorant in the market, the invention provides a preparation method of an environment-friendly deodorant enzyme, which is prepared by adopting a modern biological fermentation engineering technology, taking natural plants with a deodorization function as a culture medium, screening special beneficial microbial strains and performing fermentation treatment, wherein the environment-friendly deodorant enzyme comprises the natural plants with a deodorization function, breeding compound microbial strains, a special culture medium, a flora coordination and growth promotion factor and the like. The deodorizing ferment can reduce odor by decomposing odor substances such as ammonia, hydrogen sulfide, methyl mercaptan, trimethylamine, etc.
As shown in fig. 1, the preparation method of the environment-friendly deodorant enzyme provided by the invention comprises the following process flows:
adding natural plants, strains and nutrient substances into a fermentation tank, adding drinking water, sealing, and activating in a shade place at 15-40 ℃ for 1-15 days to obtain a bacterial liquid; wherein the natural plant comprises one or more of herba Menthae, lignum Cinnamomi Camphorae, YOUJIELI tree and tea tree; the strain comprises more than two of lactobacillus, lactobacillus plantarum, bacillus subtilis and saccharomycetes; the nutrient substances comprise more than two of glucose, lactose, brown sugar, inorganic salt, starch, beef extract, yeast powder, yeast extract, peptone, agar, water, citric acid, lactic acid, acetic acid, sodium hydroxide, potassium hydroxide and phosphate buffer salt;
and filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme.
The environment-friendly deodorant enzyme prepared by the preparation method has the advantages that the concentration of the environment-friendly deodorant enzyme is improved through the physiological metabolism of microbial strains and natural plant enzymes, the effective dilution multiple reaches 1000 times, the using amount in a large range is greatly reduced, the cost in transportation and storage can be reduced, and the cost performance of the product is finally greatly improved.
In one embodiment, the natural plants, preferably those capable of emitting natural fragrance, include one or more of herba Menthae, lignum Cinnamomi Camphorae, Equisetum Chinense L, and tea tree; wherein, the stem leaves of the mint have special fragrance and can emit strong mint special smell; the camphorwood contains organic components such as camphor, alkane, phenol, alkene and safrole in aromatic volatile oil (camphor oil), and can emit the smell of the camphorwood; tea trees, especially tea flowers, can give off a faint scent.
The natural plant can be whole tree of herba Menthae, lignum Cinnamomi Camphorae, YOUJIELI tree and tea tree; or the natural plants can be leaves of plants such as herba Menthae, radix Cinnamomi Camphorae, YOUJIELI tree and tea tree; or the natural plants can be bark of plants such as herba Menthae, radix Cinnamomi Camphorae, YOUJIELI tree and tea tree; or the natural plants can be petals of herba Menthae, radix Cinnamomi Camphorae, YOUJIELI tree and tea tree; or the natural plants can also be fruits of mint, camphor, eucalyptus, tea tree, etc.; or the natural plants can be roots of mint, camphor, eucalyptus, tea tree and other tree species; or the natural plants can be mixture of various parts of plants such as herba Menthae, radix Cinnamomi Camphorae, YOUJIELI tree and tea tree.
The natural plants can be one or more of plants, leaves, barks, petals or fruits of the corresponding species of the above-mentioned mint, camphor, eucalyptus, tea tree and the like.
In another embodiment, the natural plants may further include one or more of the corresponding natural plant enzymes of peppermint, camphor, eucalyptus, and tea.
Preferably, in one embodiment, the natural plants include peppermint, camphor, and tea tree; alternatively, in one embodiment, the natural plant comprises cinnamomum camphora and tea tree; alternatively, in one embodiment, the natural plant comprises peppermint and tea tree; or in one embodiment, the natural plant comprises cinnamomum camphora, eucalyptus, and tea tree; or in one embodiment, the natural plant is Cinnamomum camphora alone.
In one embodiment, in the preparation process of the environment-friendly deodorant enzyme, the selected strains are microbial strains which survive in culture media of natural plants with different contents and deodorization effects through continuous screening and cultivation, and the microbial strains with the most stable genetic character and the best deodorization effect are finally screened out to serve as the production strains. The strain comprises two or more of lactobacillus, lactobacillus plantarum, bacillus subtilis and yeast. Wherein, the lactobacillus is an anaerobic bacterium, which can keep living by the sugar in the plant, has the function of reducing the pH value and can inhibit the generation of nitrite; the lactobacillus plantarum belongs to fermentation lactic acid bacteria, can produce a large amount of acid, and the produced acidic substances can degrade heavy metals; the bacillus subtilis can secrete a large amount of chitinase, and the chitinase can decompose toxic and harmful substances in afternoon nap to play a role in purifying water quality; the yeast plays a role in expanding fibrous tissues in the process of rotting and fermenting animals, plants and the like.
Preferably, in one embodiment, the bacterial species include lactic acid bacteria, lactobacillus plantarum, and bacillus subtilis; or in one embodiment, the strains comprise lactobacillus plantarum and bacillus subtilis; or in one embodiment, the bacterial species comprises lactic acid bacteria and bacillus subtilis; or in one embodiment, the bacterial species comprises lactic acid bacteria and lactobacillus plantarum; or in one embodiment, the strains comprise lactic acid bacteria, bacillus subtilis and yeast; or in one embodiment, the bacterial species comprises lactic acid bacteria and yeast.
In one embodiment, the nutrient is a nutrient essential for the proliferation and growth of bacteria and/or microorganisms; in the application of the invention, the nutrient substances are used as main components of the culture medium and comprise more than two of glucose, lactose, brown sugar, inorganic salt, starch, beef extract, yeast powder, yeast extract, peptone, agar, water, citric acid, lactic acid, acetic acid, sodium hydroxide, potassium hydroxide and phosphate buffer salt. Preferably, the nutrients include glucose, starch, yeast powder, yeast water, citric acid, sodium hydroxide, and phosphate buffer salts.
In yet another embodiment, the added drinking water is activated, preferably water free of disinfectants, antibiotics, synthetic antibacterial drugs. Preferably, original ecological water such as well water, mountain stream water, mineral water and the like which are subjected to impurity removal and sterilization or tap water which is subjected to impurity removal and sterilization is adopted.
In one embodiment, in the preparation method of the environmentally friendly deodorant enzyme, the weight ratio of the natural plant to the nutrient is 1: 10-1: 100000; preferably, the weight ratio of the natural plants to the nutrient substances is 1: 5-1: 50000. Preferably, in one embodiment, the weight ratio of the natural plant to the nutrient is 1: 4-5: 3. Further, in one embodiment, the weight ratio of the natural plants to the nutrient substances is 1: 1-1: 6.
In one embodiment, in the preparation method of the environment-friendly deodorant enzyme, the ratio of the strain to the nutrient is 1:10 to 1: 100000; preferably, the weight ratio of the strain to the nutrient is 1: 5-1: 50000. Preferably, in one embodiment, the ratio of the strain to the nutrient is 1: 3-1: 60 by weight. Further, in one embodiment, the ratio of the strain to the nutrient is 1: 10-1: 30 by weight.
In one embodiment, in the preparation method of the environment-friendly deodorant enzyme, the amount of the drinking water is 1 to 1000 times of the total weight of the natural plants, the strains and the nutrients; preferably, the adding amount of the drinking water is 2-500 times of the total weight of the natural plants, the strains and the nutrient substances. Preferably, in one embodiment, the amount of the drinking water is 1-9.5 times of the total weight of the natural plants, the strains and the nutrients.
As a preferred embodiment of the present invention, the preparation method of the environment-friendly deodorant enzyme comprises the following process steps:
adding natural plants, strains and nutrient substances into a fermentation tank, adding drinking water, sealing, and activating in a shade place at the temperature of 20-35 ℃ for 5-10 days to obtain a bacterial liquid; filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme; wherein the natural plant comprises peppermint, camphor and tea eucalyptus; the strain comprises lactobacillus plantarum and bacillus subtilis; the nutrient substances comprise glucose, starch, beef extract, yeast water, peptone, agar, citric acid, sodium hydroxide and phosphate buffer salt. Furthermore, the natural plant can be selected from a whole tree such as mint, camphor and tea eucalyptus, including leaves, branches, trunks, barks, roots, petals (if any), fruits (if any), and the like, or can be a part of the natural plant such as camphor trunks, mint leaves, tea eucalyptus, and the like; the drinking water is well water with impurity removal, sand removal and sterilization; the weight ratio of the natural plant to the nutrient substance is 1: 5-1: 50000; the weight ratio of the strain to the nutrient is 1: 5-1: 50000; the adding amount of the drinking water is 2-500 times of the total weight of the natural plants, the strains and the nutrient substances.
As a further preferred embodiment of the present application, the preparation method of the environment-friendly deodorant enzyme comprises the following process steps:
adding natural plants, strains and nutrients into a fermentation tank, adding drinking water, sealing, and activating in shade at 25 deg.C for 7 days to obtain bacterial liquid;
filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme; wherein the natural plants include herba Menthae, Cinnamomum camphora, and tea tree; the strain comprises lactobacillus, lactobacillus plantarum, bacillus subtilis and saccharomycetes; the nutrient substances comprise glucose, starch, yeast powder, fermented water, citric acid, sodium hydroxide and phosphate buffer salt. Furthermore, the natural plant can be selected from a whole tree such as mint, camphor, tea tree and the like, wherein the whole tree comprises leaves, branches, trunks, barks, roots, petals (if any), fruits (if any) and the like, and can also be a part of the natural plants, such as mint leaves, camphor barks and trunks, tea branches, petals and the like; the drinking water adopts mountain stream water with impurity removal, sand removal and sterilization; the weight ratio of the natural plants to the nutrient substances is 1: 5000; the weight ratio of the strain to the nutrient is 1: 5000; the adding amount of the drinking water is 20 times of the total weight of the natural plants, the strains and the nutrient substances.
The invention further provides application of the environment-friendly deodorant enzyme in preparation of deodorants for garbage disposal sites, garbage transfer stations, garbage landfills, sewage disposal sites, farms and toilets.
When the deodorant is used, a certain amount of environment-friendly enzyme is taken according to needs, deionized water is added for dilution, then the diluted enzyme is splashed into sewage, and odor substances are decomposed by the strains, so that the deodorant effect is achieved. Wherein the weight ratio of the environment-friendly deodorizing enzyme to the deionized water is 1: 50-1: 400. In one embodiment, the weight ratio of the environmentally-friendly deodorizing ferment to the deionized water is 1: 50; for example, 1g of the environment-friendly deodorant enzyme is taken, 50g of deionized water is taken to dilute 1g of the environment-friendly deodorant enzyme, and a corresponding environment-friendly deodorant enzyme solution is prepared. In one embodiment, the weight ratio of the environmentally friendly deodorant enzyme to the deionized water is 1:400, for example, if 1g of the environmentally friendly deodorant enzyme is taken, 400g of the deionized water is required to dilute 1g of the environmentally friendly deodorant enzyme to prepare the corresponding environmentally friendly deodorant enzyme solution. In one embodiment, the weight ratio of the environmentally friendly deodorant enzyme to the deionized water is 1:200, for example, if 1g of the environmentally friendly deodorant enzyme is taken, 200g of the deionized water is required to dilute 1g of the environmentally friendly deodorant enzyme to prepare the corresponding environmentally friendly deodorant enzyme solution.
The following examples are given to illustrate the present invention in detail with respect to the effect of the test
Example 1
Adding 100g of natural plants, 20g of strains and 60g of nutrient substances into a 5-liter fermentation tank;
adding 1500g of well water subjected to impurity removal, sand removal and sterilization into the fermentation tank, and then sealing the fermentation tank;
placing the fermentation tank in a shade place at 15 ℃, and performing activation treatment for 1 day to obtain a bacterial liquid;
filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme;
wherein:
the natural plants comprise 1:1 weight ratio of mint leaves and eucalyptus leaves and branches; cutting natural plants into small sections, and adding the small sections into a fermentation tank;
the strain comprises lactobacillus plantarum, bacillus subtilis and saccharomycetes in a weight ratio of 1:1: 1;
the nutrient substances comprise lactose, brown sugar, inorganic salt, starch, yeast powder, peptone, citric acid, lactic acid and potassium hydroxide, namely phosphate buffer salt, in a weight ratio of 1:1:1:1:1:1:1:1:1: 1.
Example 2
Adding 150g of natural plants, 15g of strains and 800g of nutrient substances into a 20L fermentation tank;
6000g of mountain stream water subjected to impurity removal, sand removal and sterilization is added into the fermentation tank, and then the fermentation tank is sealed;
placing the fermentation tank in a shade place at 40 ℃, and performing activation treatment for 15 days to obtain a bacterial liquid;
filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme;
wherein:
the natural plants comprise mint leaves and stems, camphor trunks and barks, eucalyptus enzymes, and tea tree leaves and branches in a weight ratio of 1:2:0.5: 1; cutting natural plants into small sections, and adding the small sections into a fermentation tank;
the strains comprise lactobacillus, lactobacillus plantarum, bacillus subtilis and saccharomycetes in a weight ratio of 1:2:2: 1;
the nutrient substances comprise glucose, inorganic salt, starch, beef extract, yeast powder, peptone, citric acid, sodium hydroxide and phosphate buffer salt in a weight ratio of 1:0.5:1:2:0.5:0.5:1:1.5: 1.
Example 3
Adding 100g of natural plants, 15g of strains and 350g of nutrient substances into a 5-liter fermentation tank;
adding 3000g of well water subjected to impurity removal, sand removal and sterilization into the fermentation tank, and then sealing the fermentation tank;
placing the fermentation tank in a shade place at 25 ℃, and performing activation treatment for 7 days to obtain a bacterial liquid;
filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme;
wherein:
the natural plants comprise mint whole trees, camphor fruits and barks, eucalyptus barks and tea tree branches in a weight ratio of 2:1:1: 1; cutting natural plants into small sections, and adding the small sections into a fermentation tank;
the strains comprise lactobacillus, lactobacillus plantarum and bacillus subtilis in a weight ratio of 1:2: 1;
the nutrient substances comprise lactose, brown sugar, inorganic salt, starch, beef extract, agar, water, acetic acid and sodium hydroxide in a weight ratio of 2:1:1:2:1.5:0.5:1:2: 1.
Example 4
Adding 200g of natural plants, 30g of strains and 800g of nutrient substances into a 20-liter fermentation tank;
10300g of mountain stream water which is subjected to impurity removal, sand removal and sterilization is added into a fermentation tank, and then the fermentation tank is sealed;
placing the fermentation tank in a shade place at 35 ℃, and performing activation treatment for 10 days to obtain a bacterial liquid;
filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme;
wherein:
the natural plant is the whole mint tree; cutting natural plants into small sections, and adding the small sections into a fermentation tank;
the strains comprise lactobacillus, lactobacillus plantarum, bacillus subtilis and saccharomycetes in a weight ratio of 1:1:0.5: 1;
the nutrient substances comprise brown sugar, inorganic salt, starch, beef extract, yeast extract, agar, citric acid, sodium hydroxide and phosphate buffer salt in a weight ratio of 2:1:1:2:1:0.5:1:2: 1.
Example 5
Adding 100g of natural plants, 10g of strains and 100g of nutrient substances into a 5-liter fermentation tank;
adding 2000g of mineral water subjected to impurity removal, sand removal and sterilization into the fermentation tank, and then sealing the fermentation tank;
placing the fermentation tank in a shade place at 20 ℃, and performing activation treatment for 5 days to obtain a bacterial liquid;
filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme;
wherein:
the natural plants comprise mint branches, camphor barks, eucalyptus leaves, tea tree leaves and tea tree enzymes in a weight ratio of 1:1:2: 1; cutting natural plants into small sections, and adding the small sections into a fermentation tank;
the strain comprises lactobacillus and lactobacillus plantarum with the weight ratio of 1: 2;
the nutrient substances comprise glucose, brown sugar, starch, beef extract, yeast extract, agar, lactic acid, acetic acid, sodium hydroxide and phosphate buffer salt in a weight ratio of 1:2:1:2:1:0.5:1:1:2: 1.
Comparative example
Adding 5g of leaven, 10g of fresh fruits and vegetables, 100g of water and 5g of brown sugar into a 2-liter fermentation tank, placing the fermentation tank in a shade place at room temperature for fermentation for 100 days, and filtering the fermentation liquor to obtain fruit and vegetable ferment liquid; wherein the fresh fruits and vegetables include spinach leaf, celery leaf and stem, apple peel and fruit pulp.
The following are experimental comparative tests
In examples 1 to 5, 100ml of the eco-deodorant enzyme was placed in 5 500ml volumetric flasks and diluted to 500ml with deionized water, respectively.
In the comparative example, 100ml of fruit and vegetable ferment liquid is taken and placed in a 500ml volumetric flask and diluted to 500ml with deionized water.
500ml of treated sewage of a Shenzhen city front sea sewage treatment plant is taken and placed in a volumetric flask of 2500ml, the treated sewage is diluted to 2500ml by deionized water, then 6 measuring cups of 500ml are taken, and 200ml of diluted sewage is respectively taken and placed in 6 measuring cups. Then, the environmental protection deodorization enzymes and the fruit and vegetable enzyme solutions in examples 1 to 5 are respectively used for treating the sewage from water quality indexes such as smell, turbidity, alkalinity, total phosphorus, pH value and the like, and after standing for 15 days, the detection results are as follows in table 1:
table 1: urban sewage treatment water quality index detection result
From the above table 1, it can be seen that the detection indexes of examples 1 to 5 are all better than the water quality detection indexes of the comparative examples, for example, ① water quality odor still exists in the comparative examples, that is, the fruit and vegetable ferment liquid does not undergo micro-chemical decomposition or is not ideal enough to decompose ammonia nitrogen, hydrogen sulfide and the like in the sewage during the sewage treatment, ② water turbidity of the examples of the present invention is also better than the comparative examples, that is, the sewage treated by the environmental protection deodorizing ferment of examples 1 to 5 has clear water quality, ③ sewage treated by the environmental protection deodorizing ferment of examples 1 to 5 has total alkalinity and total phosphorus degree which are also obviously better than the comparative examples.
It should be understood that the above description is illustrative of the preferred embodiment of the present invention and is not to be construed as limiting the scope of the invention, which is defined by the appended claims.
Claims (10)
1. The preparation method of the environment-friendly deodorant enzyme is characterized by comprising the following steps of:
adding natural plants, strains and nutrient substances into a fermentation tank, adding drinking water, sealing, and activating in a shade place at 15-40 ℃ for 1-15 days to obtain a bacterial liquid; wherein the natural plant comprises one or more of herba Menthae, lignum Cinnamomi Camphorae, YOUJIELI tree and tea tree; the strain comprises more than two of lactobacillus, lactobacillus plantarum, bacillus subtilis and saccharomycetes; the nutrient substances comprise more than two of glucose, lactose, brown sugar, inorganic salt, starch, beef extract, yeast powder, yeast extract, peptone, agar, water, citric acid, lactic acid, acetic acid, sodium hydroxide, potassium hydroxide and phosphate buffer salt;
and filtering and extracting the activated bacterial liquid to obtain the environment-friendly deodorant enzyme.
2. The preparation method according to claim 1, wherein the weight ratio of the natural plant to the nutrient is 1:10 to 1: 100000.
3. The preparation method according to claim 2, wherein the weight ratio of the natural plant to the nutrient is 1:5 to 1: 50000.
4. The method according to claim 1, wherein the ratio of the strain to the nutrient is 1:10 to 1:100000 by weight.
5. The preparation method according to claim 4, wherein the ratio of the strain to the nutrient is 1:5 to 1: 50000.
6. The method according to claim 1, wherein the amount of the drinking water added is 1 to 1000 times of the total weight of the natural plants, strains and nutrients.
7. The preparation method according to claim 6, wherein the amount of the drinking water added is 2 to 500 times of the total weight of the natural plants, strains and nutrients.
8. The method according to claim 1, wherein the activation temperature is 20 to 35 ℃ and the activation time is 5 to 10 days.
9. An environmentally friendly deodorant enzyme prepared by the preparation method according to any one of claims 1 to 8.
10. Use of the environmentally friendly deodorant enzyme according to claim 9 for preparing a deodorant for garbage disposal sites, garbage transfer stations, landfill sites, sewage disposal sites, livestock farms, and toilets.
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| CN112156644A (en) * | 2020-10-27 | 2021-01-01 | 深圳市淳睿科技发展有限公司 | Environment-friendly enzyme deodorant for organic fertilizer production workshop and using method thereof |
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