CN102765972B - Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent - Google Patents
Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent Download PDFInfo
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- CN102765972B CN102765972B CN201210246276.9A CN201210246276A CN102765972B CN 102765972 B CN102765972 B CN 102765972B CN 201210246276 A CN201210246276 A CN 201210246276A CN 102765972 B CN102765972 B CN 102765972B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses an organic garbage decomposing bacterium agent, as well as a preparation method and an application of the organic garbage decomposing bacterium agent. The bacterium agent is composed of 55-60% by weight of I compound bacterium agent and 40-45% by weight of II compound bacterium agent, wherein the I compound bacterium agent as well as the II compound bacterium agent are composed of bacterium liquid and a carrier, and respectively adsorbed on the carrier by the bacterium liquid; and the ratio of the bacterium liquid to the carrier is 2-3 hundred million bacteria to per gram of the carrier. The organic garbage decomposing bacterium agent is uniformly added according to 0.5-1% of the weight of an organic garbage heap; and the synergistic effect of various bacteria is capable of effectively accelerating the decomposition speed of the organic garbage and improving the quality of produced organic fertilizer. Due to use of the organic garbage decomposing bacterium agent, the pollution problems such as wastewater overflow, odor smell and mosquitoes flying around caused by the organic garbage stacking are effectively solved, and high-quality organic fertilizer can be produced.
Description
Technical field
The present invention relates to environmental technology field, especially a kind of organic waste decomposing microbial inoculum and its preparation method and application.
Background technology
Organic waste, as the agricultural such as stalk, leaf refuse, the daily rubbish such as the remaining hogwash in kitchen, pericarp, dish leaf and feces of livestock and poultry, food waste residue etc., if directly bank up, not only can cause the pollution problems such as sewage crossing current, breath malodor and mosquito disorderly fly, and people's orthobiosis is produced to greatly impact, affect city image.And in organic waste, conventionally contain a large amount of Mierocrystalline celluloses, organic matter and N, P, K and other trace elements, and there is the features such as easy decomposition, calorific value be low, if it is not recycled, can cause the wasting of resources.
China's organic waste has that kind is many, source is wide, output large and the feature of complicated component, and the annual production of organic waste is with the speed increase of 8-10%, and therefore how effectively processing organic waste becomes urgent problem; On the other hand, China's cultivated land resource is in short supply, and the fundamental realities of the country of having a large population and a few land causes soil nutrient to be exhausted, this just need to apply a large amount of fertilizer in ploughing.But life-time service chemical fertilizer can cause the problems such as soil compaction, disease and pest aggravation and water pollution.Because fertilizer has improvement soil, strengthen the ability of crop opposing disease and pest and environmental protection, the advantage such as pollution-free, the demand of fertilizer is sharply increased.
To sum up, at present in the urgent need to a kind of organic waste application technology as the second resource, to alleviate the harm of organic waste to environment, and realize the recycle of organic waste, unmanageable organic waste is turned waste into wealth, realize energy-saving and emission-reduction and low-carbon economy developing goal.
Summary of the invention
In order to overcome the above-mentioned deficiency of prior art, the invention provides a kind of organic waste decomposing microbial inoculum and its preparation method and application, use this microbial inoculum and method greatly to improve organic fertilizer fermentation production rate, shorten fermentation period, realize waste Rapid Circulation recycling, solve the organic waste pollution problem producing in process of banking up, and used the fertilizer of its generation can to improve soil.
Technical scheme of the present invention is as follows:
A kind of organic waste decomposing microbial inoculum, this microbial inoculum is made up of I composite fungus agent and II composite fungus agent, wherein I composite fungus agent accounts for 55-60% weight fraction, II composite fungus agent accounts for 40-45% weight fraction, I composite fungus agent and II composite fungus agent are made up of bacterium liquid and carrier, be adsorbed on carrier and formed by bacterium liquid respectively, the ratio of bacterium liquid and carrier is 2-3 hundred million bacterium number/g carriers;
Photosynthetic bacterium, genus bacillus, series bacillus, product yellowish fiber Zymomonas mobilis, blown-ball Azotobacter and Pseudomonas fluorescens that wherein I composite fungus agent is 2:2:1:1:1:1 by the ratio of colony-forming unit form; Wood that II composite fungus agent is 4:4:2:1 by the ratio of colony-forming unit is mould, Candida utilis, mould and purple variant streptomycete form.
Preferably, described carrier is straw powder.
The preparation method who the invention also discloses a kind of above-mentioned organic waste decomposing microbial inoculum, is characterized in that:
(1) in different culture media, inoculate respectively the corresponding bacterium of I composite fungus agent and II composite fungus agent, in 30 ℃ of shaking tables, 1-2 days is cultivated in 180r/min concussion afterwards, and after cultivation, dilution forms the bacterium liquid of 2-3 hundred million/ml;
(2) in the ratio of 2-3 hundred million/g, different bacterium liquid is adsorbed onto respectively on carrier, mix respectively according to the inoculating proportion of I composite fungus agent and II composite fungus agent afterwards, obtain I composite fungus agent and II composite fungus agent;
(3) be that 55-60% and 40-45% mix according to weight fraction respectively by I composite fungus agent and II composite fungus agent, obtain organic waste decomposing microbial inoculum.
The present invention also provides the application of a kind of above-mentioned organic waste decomposing microbial inoculum aspect processing organic waste and manufacture fertilizer.
The present invention also provides a kind of above-mentioned organic waste decomposing microbial inoculum processing organic waste and manufactures the method for fertilizer, evenly add this organic waste decomposing microbial inoculum by the 0.5-1% of organic waste heap body weight, make heap, keep heap body water-content at 55-65%, every 6-7 days turning 1 time, turning 3-4 time altogether, waiting to have fermented just obtains efficient biologic-organic fertilizer.
Compared with prior art, beneficial effect of the present invention is as follows:
1, the present invention adopts complex micro organism fungicide fermentative degradation organic waste.In this microbial inoculum, contain a large amount of cellulose degradation microorganisms, the enzyme that these microorganisms secrete in degradation process has complementarity and synergy obviously, can be by organic waste fast degradation, thus improve organic fertilizer production efficiency;
2, this fertilizer has also added other beneficial microorganism.Purple variant streptomycete has the function of P and K decomposing, can improve soil nutrient status; Blown-ball Azotobacter can strengthen soil nitrogen fixing capacity, in volume increase, reduces fertilizer amount; Yeast can reduce the generation of composting fermentation process stink, improves fertilizer quality;
3, microbial inoculum of the present invention can obviously shorten degradation time.Add during the fermentation microbial inoculum of the present invention, make a fermentation period of compost shorten to 6-7 days, compare control fermentation and shortened 2-3 days, fermentation period is shortened to 30% left and right, can improve largely the production rate of fertilizer, reduction production cost, is convenient to the use of actual production;
4, technique of the present invention is simple, has realized the harmless treatment of organic waste, has solved a difficult problem for current organic waste difficult degradation, produces the fertilizer of improvement soil simultaneously, has far-reaching social effect and economic worth.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of temperature variation in organic waste compost maturity process in the embodiment of the present invention 1.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be appreciated that, these embodiment are only for the present invention is described, and are not intended to limit the scope of the invention.Those skilled in the art make according to the present invention in actual applications improvement and adjustment, still belong to protection scope of the present invention.
A kind of organic waste decomposing microbial inoculum, formed by I composite fungus agent and II composite fungus agent, wherein I composite fungus agent accounts for 55-60% weight fraction, II composite fungus agent accounts for 40-45% weight fraction, I composite fungus agent and II composite fungus agent are made up of bacterium liquid and carrier, be adsorbed on carrier and formed by bacterium liquid respectively, the ratio of bacterium liquid and carrier is 2-3 hundred million bacterium number/g carriers;
Photosynthetic bacterium, genus bacillus, series bacillus, product yellowish fiber Zymomonas mobilis, blown-ball Azotobacter and Pseudomonas fluorescens that wherein I composite fungus agent is 2:2:1:1:1:1 by the ratio of colony-forming unit form; Wood that II composite fungus agent is 4:4:2:1 by the ratio of colony-forming unit is mould, Candida utilis, mould and purple variant streptomycete form.Be elaborated as an example of a specific embodiment example below.
Production Example
In the present embodiment, the preparation method of organic waste decomposing microbial inoculum is as follows:
(1) in different culture media, inoculate respectively the corresponding bacterium of I composite fungus agent and II composite fungus agent, in 30 ℃ of shaking tables, 180r/min concussion is cultivated 1 day afterwards, after cultivation, dilution forms the bacterium liquid of appropriate concentration, and every milliliter of bacterium number is about 200,000,000, i.e. 200,000,000/ml;
Photosynthetic bacteria culture medium is: NH
4cl 1.0g/L, K
2hPO
40.5g/L, MgCl
20.2g/L, NaCl 2.0g/L, yeast extract paste 0.1g/L, pH 7.0.
Pseudomonas fluorescens substratum is: extractum carnis 3.0g/L, peptone 10.0g/L, NaCl 5.0g/L, pH 7.0-7.2.
Genus bacillus, purple variant streptomycete substratum are: yeast extract paste 2.0g/L, glucose 8.0g/L, K
2hPO
46.5g/L, NaNO
33.0g/L, NH
4cl 1.0g/L, NaCl 3.0g/L, MgSO
40.05g/L, MnSO
40.01g/L, FeSO
47H
2o 0.005g/L, pH 7.5.
Series bacillus substratum: Tryptones 15.0g/L, soya peptone 5.0g/L, NaCl 5.0g/L, pH7.3 ± 0.2.
Mould, the mould substratum of wood: wheat bran 10.0g/L, sucrose 10.0g/L, KH
2pO
44.0g/L, MgSO
40.3g/L, natural pH.
Candida utilis substratum: malt extract powder 130g/L, paraxin 0.1g/L, pH 6.0 ± 0.2.
Blown-ball Azotobacter substratum: yeast extract paste 0.5g/L, N.F,USP MANNITOL 20g/L, KH
2pO
40.2g/L, K
2hPO
40.8g/L, MgSO
47H
2o 0.2g/L, CaSO
42H
2o 0.1g/L, pH 7.2.
(2) in the ratio of 200,000,000/g, different bacterium liquid is adsorbed onto to carrier as in straw powder, mix respectively according to each inoculating proportion of I composite fungus agent and II composite fungus agent, obtain I composite fungus agent and II composite fungus agent;
(3) I composite fungus agent and II composite fungus agent are mixed according to weight fraction 55% and 45%, obtain organic waste decomposing microbial inoculum.
Above used carrier is straw powder, and in addition, carrier also can use any this area such as wheat bran, wood chip to commonly use and available carrier; In addition, I composite fungus agent and II composite fungus agent also can be adsorbed on different carriers.
Application examples
The application of decomposing microbial inoculum in organic waste compost fermentation
Organic waste is air-dry, be crushed to 5cm left and right through disintegrating apparatus, form broken section.The heap body volume of pressing 2m × 2m × 1.5m, evenly adds above-mentioned organic waste decomposing microbial inoculum with 1% of organic waste weight, makes heap.Keep organic junk-heap body water-content in 65% left and right, turning 1 time in every 7 days, turning 3 times altogether, waiting to have fermented just obtains efficient biologic-organic fertilizer.When concrete enforcement, weight fraction, heap body water content, turning cycle and the number of times of the organic waste size of the pulverizing of above-mentioned fermenting process, heap body volume, organic waste decomposing microbial inoculum all can change with concrete environment for use, do not limit herein.
In composting fermentation process, from the 1st day, measured the temperature of heap body every day, obtains the temperature variation curve of a complete decomposed period (1-22 days) as Fig. 1.In figure, can find out, add the experimental group (JFB-2) of the organic waste decomposing microbial inoculum of the present embodiment, in fermenting process, pile the temperature of body apparently higher than control group, and can obviously shorten fermentation period, thereby make fermentation more abundant, output fertilizer quality is higher.
Production Example
In the present embodiment, the preparation method of organic waste decomposing microbial inoculum is as follows:
(1) in different culture media, inoculate respectively the corresponding bacterium of I composite fungus agent and II composite fungus agent, in 30 ℃ of shaking tables, 180r/min concussion is cultivated 2 days afterwards, after cultivation, dilution forms the bacterium liquid of appropriate concentration, and every milliliter of bacterium number is about 300,000,000, i.e. 300,000,000/ml;
Photosynthetic bacteria culture medium is: NH
4cl 1.0g/L, K
2hPO
40.5g/L, MgCl
20.2g/L, NaCl 2.0g/L, yeast extract paste 0.1g/L, pH 7.0.
Pseudomonas fluorescens substratum is: extractum carnis 3.0g/L, peptone 10.0g/L, NaCl 5.0g/L, pH 7.0-7.2.
Genus bacillus, purple variant streptomycete substratum are: yeast extract paste 2.0g/L, glucose 8.0g/L, K
2hPO
46.5g/L, NaNO
33.0g/L, NH
4cl 1.0g/L, NaCl 3.0g/L, MgSO
40.05g/L, MnSO
40.01g/L, FeSO
47H
2o 0.005g/L, pH 7.5.
Series bacillus substratum: Tryptones 15.0g/L, soya peptone 5.0g/L, NaCl 5.0g/L, pH7.3 ± 0.2.
Mould, the mould substratum of wood: wheat bran 10.0g/L, sucrose 10.0g/L, KH
2pO
44.0g/L, MgSO
40.3g/L, natural pH.
Candida utilis substratum: malt extract powder 130g/L, paraxin 0.1g/L, pH 6.0 ± 0.2.
Blown-ball Azotobacter substratum: yeast extract paste 0.5g/L, N.F,USP MANNITOL 20g/L, KH
2pO
40.2g/L, K
2hPO
40.8g/L, MgSO
47H
2o 0.2g/L, CaSO
42H
2o 0.1g/L, pH 7.2.
(2) in the ratio of 300,000,000/g, different bacterium liquid is adsorbed onto to carrier as in straw powder, mix respectively according to each inoculating proportion of I composite fungus agent and II composite fungus agent, obtain I composite fungus agent and II composite fungus agent;
(3) I composite fungus agent and II composite fungus agent are mixed according to weight fraction 60% and 40%, obtain organic waste decomposing microbial inoculum.
Above used carrier is straw powder, and in addition, carrier also can use any this area such as wheat bran, wood chip to commonly use and available carrier.
Application examples
The application of decomposing microbial inoculum in organic waste compost fermentation
Organic waste is air-dry, be crushed to 5cm left and right through disintegrating apparatus, form broken section.The heap body volume of pressing 2m × 2m × 1.5m, evenly adds above-mentioned organic waste decomposing microbial inoculum with 0.5% of organic waste weight, makes heap.Keep organic junk-heap body water-content in 55% left and right, turning 1 time in every 6 days, turning 4 times altogether, waiting to have fermented just obtains efficient biologic-organic fertilizer.When concrete enforcement, weight fraction, heap body water content, turning cycle and the number of times of the organic waste size of the pulverizing of above-mentioned fermenting process, heap body volume, organic waste decomposing microbial inoculum all can change with concrete environment for use, do not limit herein.
The preferred embodiment of the present invention is just for helping to set forth the present invention.Preferred embodiment does not have all details of detailed descriptionthe, and also not limiting this invention is only described embodiment.Obviously,, according to the content of this specification sheets, can make many modifications and variations.These embodiment are chosen and specifically described to this specification sheets, is in order to explain better principle of the present invention and practical application, thereby under making, technical field technician can utilize the present invention well.The present invention is only subject to the restriction of claims and four corner and equivalent.
Claims (4)
1. an organic waste decomposing microbial inoculum, it is characterized in that, this microbial inoculum is made up of I composite fungus agent and II composite fungus agent, wherein I composite fungus agent accounts for 55-60% weight fraction, II composite fungus agent accounts for 40-45% weight fraction, I composite fungus agent and II composite fungus agent are made up of bacterium liquid and carrier, are adsorbed on carrier and are formed respectively by bacterium liquid, and the ratio of bacterium liquid and carrier is 2-3 hundred million bacterium number/g carriers;
Photosynthetic bacterium, genus bacillus, series bacillus, product yellowish fiber Zymomonas mobilis, blown-ball Azotobacter and Pseudomonas fluorescens that wherein I composite fungus agent is 2:2:1:1:1:1 by the ratio of colony-forming unit form; Wood that II composite fungus agent is 4:4:2:1 by the ratio of colony-forming unit is mould, Candida utilis, mould and purple variant streptomycete form; Wherein, described organic waste decomposing microbial inoculum is prepared by following methods:
(1) in different culture media, inoculate respectively the corresponding bacterium of I composite fungus agent and II composite fungus agent, in 30 ℃ of shaking tables, 1-2 days is cultivated in 180r/min concussion afterwards, and after cultivation, dilution forms the bacterium liquid of 2-3 hundred million/ml;
(2) in the ratio of 2-3 hundred million/g, different bacterium liquid is adsorbed onto respectively on carrier, mix respectively according to the inoculating proportion of I composite fungus agent and II composite fungus agent afterwards, obtain I composite fungus agent and II composite fungus agent;
(3) be that 55-60% and 40-45% mix according to weight fraction respectively by I composite fungus agent and II composite fungus agent, obtain organic waste decomposing microbial inoculum.
2. organic waste decomposing microbial inoculum as claimed in claim 1, is characterized in that, described carrier is straw powder.
3. an organic waste decomposing microbial inoculum claimed in claim 1 is in the application of processing aspect organic waste and manufacture fertilizer.
4. an organic waste decomposing microbial inoculum claimed in claim 1 is processed organic waste and is manufactured the method for fertilizer, it is characterized in that, evenly add this organic waste decomposing microbial inoculum by the 0.5-1% of organic waste heap body weight, make heap, keep heap body water-content at 55-65%, every 6-7 days turning 1 time, turning 3-4 time altogether, waiting to have fermented just obtains efficient biologic-organic fertilizer.
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CN103964908A (en) * | 2013-01-29 | 2014-08-06 | 都江堰惠农生物技术有限责任公司 | Compound microorganism agent for compound microorganism fermentation of organic solid wastes |
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CN105016888A (en) * | 2015-07-28 | 2015-11-04 | 上海交通大学 | Microbe based on straw waste and preparation method of fertilizer by fermentation by using microbe |
CN106282048A (en) * | 2016-08-04 | 2017-01-04 | 华侨大学 | A kind of complex microorganism preparations and its preparation method and application |
CN107244750A (en) * | 2017-08-11 | 2017-10-13 | 中国科学院地球化学研究所 | Red mud leachate processing method and three color moulds are used for the application for handling red mud percolate |
CN108178668A (en) * | 2018-01-30 | 2018-06-19 | 河南博联农业研究院有限公司 | A kind of composite microbiological fertilizer and preparation method thereof |
SG10201807584UA (en) * | 2018-09-04 | 2020-04-29 | Westcom Solutions Pte Ltd | Methods and products for biodegradation of waste |
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CN110317763B (en) * | 2019-08-02 | 2022-10-04 | 贵州师范大学 | Efficient microbial compound microbial inoculum |
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