CN101988040A - Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof - Google Patents
Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof Download PDFInfo
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- CN101988040A CN101988040A CN2009100700737A CN200910070073A CN101988040A CN 101988040 A CN101988040 A CN 101988040A CN 2009100700737 A CN2009100700737 A CN 2009100700737A CN 200910070073 A CN200910070073 A CN 200910070073A CN 101988040 A CN101988040 A CN 101988040A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention relates to a compound bactericide for efficient degradation of landscaping garbage and a preparation method thereof. The compound bactericide mainly consists of strains and a carrier, wherein the strains comprise the following components in parts by weight: 15-30 parts of fibre porphyromonas gingivalis, 10-25 parts of basidiomycetes, 5-15 parts of bacillus, 15-35 parts of aspergillus, 5-35 parts of neurospora, 8-20 parts of trichoderma, 15-20 parts of actinomycetes and 5-15 parts of saccharomycetes. The compound bactericide is fully mixed with the landscaping garbage in a fermentation device and is synergized through different bactericides under the aerobic condition, thereby the landscaping garbage degradation efficiency is improved, and the organic matter is rapidly corrupted and converted into green organic fertilizer capable of improving soil. The application of the compound bactericide for efficient degradation of landscaping garbage can effectively solve the problems of land occupation caused by land filling or pollution to the atmospheric environment caused by burning, and the like.
Description
Technical field
The present invention relates to the microbial preparation field, especially a kind of afforestation rubbish efficient degradation composite fungus agent and preparation method thereof that can be used for.
Background technology
Since ancient times, the treatment process for the treatment of fallen leaves, withered grass etc. bank up exactly, landfill or burning.The method of banking up is the simplest treatment process, and this method not only takies a large amount of land resourcess, and fallen leaves, withered grass etc. are rotted gradually in the process of banking up, and produces stench, influences the healthy of surrounding resident.Though the application of landfill method has solved land seizure and malodorous problem, because slowly degradeds in soil such as fallen leaves, withered grass, the nutrient and the degradation speed that consume in the soil are slow.Burning method is that fallen leaves, withered grass etc. are reached the purpose of minimizing by burning, and the ashes after will burning are as fertilizer.Though this incinerating method is used as fertilizer with ashes, pollutant atmosphere, it is healthy to influence people.
Continuous development along with biotechnology it is found that afforestation rubbish such as fallen leaves, withered grass are different with domestic waste, and it is made up of xylogen, Mierocrystalline cellulose etc., and these " foods " of certain micro-organisms bacterium just.These microorganisms can metabolism produce small organic molecule by " foods " such as picked-up xylogen, Mierocrystalline celluloses.This small organic molecule can be absorbed by crop in the soil, promotes plant growth, therefore can produce fertilizer by afforestation rubbish such as microbiological deterioration fallen leaves, withered grass.
Also there is a big difference in microorganism field and developed country in China, and the biological inoculum of domestic development fertilizer aspect is few, and the bacterial classification dependence on import that producer uses mostly costs an arm and a leg, and bacterial classification mortality ratio height.At present, do not seen both at home and abroad and utilized directly the degrade report of afforestation rubbish of complex microbial inoculum.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of afforestation rubbish efficient degradation composite fungus agent and preparation method thereof is provided, problems such as present afforestation rubbish microbial starter culture degradation speed is slow to solve, microbial inoculum price height, weak effect.
The present invention is achieved through the following technical solutions:
A kind of afforestation rubbish efficient degradation composite fungus agent is made of bacterial classification component and carrier, and the bacterial classification component is formed by 4 kinds in following 8 kinds of microorganisms at least, and its parts by weight are:
Cellulomonas cartae 15-30 part;
Basidiomycetes 10-25 part;
Genus bacillus 5-15 part;
Aspergillus 15-35 part;
Neurospora 5-35 part;
The mould 8-20 part of wood;
Actinomycetes 15-20 part;
Yeast 5-15 part;
Carrier comprises one or both in chitosan, rice bran, wood chip, the soil.
And described cellulomonas cartae is the dinitrogen cellulomonas cartae or produces the yellowish fiber Zymomonas mobilis;
Basidiomycetes is oyster cap fungus or flat mushroom;
Genus bacillus is Bacillus licheniformis or subtilis or bacillus natto or cured shape genus bacillus or bacillus megaterium;
Aspergillus is aspergillus niger;
Neurospora is that the mould or middle intercadence spore of coarse arteries and veins spore is mould;
Wood is mould to be that viride or healthy and free from worry wood are mould;
Actinomycetes are Nocardia bacteria or small single-cell bacteria;
Yeast is candida tropicalis or produces Ruan's candiyeast or yeast saccharomyces cerevisiae.
A kind of preparation method of afforestation rubbish efficient degradation composite fungus agent, this method may further comprise the steps:
(1) behind the inoculation of medium bacterial classification, put in 35 ℃ of shaking tables 120 rev/mins, shaking culture 48-72 hour, cultivate suitably to dilute after finishing and form bacterium liquid, making every milliliter of bacterium number is 500,000,000, i.e. 500,000,000/milliliter;
(2) the bacterium number of bacterium liquid by every gram carrier 200,000,000 is adsorbed onto on the carrier, makes the efficient degradation composite fungus agent of afforestation rubbish.
Advantage of the present invention and beneficial effect are:
1, afforestation rubbish efficient degradation composite fungus agent of the present invention is a kind of microbial inoculum that is made of many bacterial classifications, utilize conventional fermentation equipment, make itself and afforestation rubbish thorough mixing, under aerobic condition, act synergistically by different strain, directly divide the fallen leaves that are deconstructed into afforestation rubbish, withered grass etc., and will decompose after the rotten slaking of these afforestation rubbish, produce a large amount of soil ulmin, organic matter and trace element etc., constituted the fertilizer of useful plants growth, realize the transformation of changing waste into resources, really realized zero pollution, zero release.
2, the efficient degradation composite fungus agent of the prepared afforestation rubbish of the present invention, the microbial inoculum cost is low, and degradation speed is fast, can efficient degradation afforestation rubbish, make its fast rotting be degraded into fertilizer, have profound significance and realistic price.
Embodiment
The present invention is described in further detail by following examples, but the technology contents that present embodiment is narrated is illustrative, rather than determinate, should not limit to protection scope of the present invention according to this.
Embodiment 1:
A kind of afforestation rubbish efficient degradation composite fungus agent, this efficient degradation composite fungus agent mainly is made of bacterial classification and carrier, and its bacterial classification is formed and parts by weight are:
15% part of dinitrogen cellulomonas cartae, 20 parts of oyster cap fungus, 8 parts of subtilises, 15 parts of aspergillus nigers, mould 8 parts of coarse arteries and veins spore, mould 10 parts of healthy and free from worry wood, 15 parts of Nocardia bacterias, 9 parts of candida tropicalises;
Carrier is chitosan and wood chip.
The preparation method of afforestation rubbish efficient degradation composite fungus agent of the present invention may further comprise the steps:
(1) behind the inoculation of medium bacterial classification, put in 35 ℃ of shaking tables 120 rev/mins, shaking culture 48-72 hour, cultivate suitably to dilute after finishing and form bacterium liquid, making every milliliter of bacterium number is 500,000,000, i.e. 500,000,000/milliliter;
(2) the bacterium number of bacterium liquid by every gram carrier 200,000,000 is adsorbed onto on the carrier, makes the efficient degradation composite fungus agent of afforestation rubbish.
Embodiment 2:
A kind of afforestation rubbish efficient degradation composite fungus agent, this efficient degradation composite fungus agent mainly is made of bacterial classification and carrier, and its bacterial classification is formed and parts by weight are:
Produce 15 parts of yellowish fiber Zymomonas mobiliss, 20 parts of flat mushrooms, 8 parts of bacillus nattos, 15 parts of aspergillus nigers, mould 8 parts of coarse arteries and veins spore, mould 10 parts of healthy and free from worry wood, 15 parts of Nocardia bacterias, 9 parts of yeast saccharomyces cerevisiaes;
Carrier is rice bran and wood chip.
Preparation method's step is same as embodiment 1.
Embodiment 3:
A kind of afforestation rubbish efficient degradation composite fungus agent, this efficient degradation composite fungus agent mainly is made of bacterial classification and carrier, and its bacterial classification is formed and parts by weight are:
20 parts of dinitrogen cellulomonas cartaes, 15 parts of oyster cap fungus, 25 parts of aspergillus nigers, 20 parts of virides, 15 parts of Nocardia bacterias produce 5 parts of Ruan's candiyeasts;
Carrier is a rice bran.
Preparation method's step is same as embodiment 1.
Embodiment 4:
A kind of afforestation rubbish efficient degradation composite fungus agent, this efficient degradation composite fungus agent mainly is made of bacterial classification and carrier, and its bacterial classification is formed and parts by weight are:
Produce 25 parts of yellowish fiber Zymomonas mobiliss, 5 parts of bacillus megateriums, mould 30 parts of middle intercadence spore, 10 parts of virides, 15 parts of small single-cell bacterias, 15 parts of yeast saccharomyces cerevisiaes;
Carrier is a wood chip.
Preparation method's step is same as embodiment 1.
The used substratum of each bacterial classification of the present invention is:
Cellulomonas cartae, genus bacillus are cultivated and use substratum: peptone 10g, and beef leaching thing 3g, sodium-chlor 5g, distilled water 1L, pH value 7.0 places the 250ml Erlenmeyer flask respectively, and every bottle of 80ml uses behind the high pressure steam sterilization;
Basidiomycetes, yeast are cultivated and used the PDA substratum: potato is cleaned peeling be cut into small pieces, take by weighing potato 200g, distilled water 1000ml boiled 20 minutes, nine layers of filtered through gauze to 1000ml, add glucose 20g with the filtrate moisturizing that obtains, potassium primary phosphate 3g, MgSO
47H
2O 1.5 grams, vitamins B
1Trace is regulated pH value 6.0, places the 250ml Erlenmeyer flask respectively, and every bottle of 80ml uses behind the high pressure steam sterilization;
Aspergillus, wooden mould cultivation malt extract medium: wort 20g, peptone 1g, glucose 20g, distilled water 1000ml regulates pH value 5.0, places the 250ml Erlenmeyer flask respectively, and every bottle of 80ml uses behind the high pressure steam sterilization;
Neurospora is cultivated and is used minimum medium: citric acid 3.0g, potassium primary phosphate 5.0g, ammonium nitrate 2.0g, MgSO
47H
2O 0.2g, calcium chloride 0.1g, sucrose 20.0g, micro-1.0ml, distilled water 1000ml, pH value nature places the 250ml Erlenmeyer flask respectively, and every bottle of 80ml uses behind the high pressure steam sterilization;
Actinomycetes are cultivated and use substratum: Zulkovsky starch 20g, saltpetre 1g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, ferrous sulfate 0.01g, distilled water 1000mL regulates pH value 7.2-7.4, place the 250ml Erlenmeyer flask respectively, every bottle of 80ml uses behind the high pressure steam sterilization;
Trace element: citric acid H
2O 500mg, ZnSO
47H
2O 500mg, Fe (NH
4)
2(SO
4)
26H
2O100mg, CuSO
45H
2O 25mg, MnSO
44H
2O 5mg, H
3BO
35mg, Na
2MoO
42H
2O5mg, adding distil water is standby to 100ml.
Above-mentioned substratum adds culture volume than 10% afforestation rubbish in culturing process.
Claims (3)
1. afforestation rubbish efficient degradation composite fungus agent, it is characterized in that: be made of bacterial classification component and carrier, the bacterial classification component is formed by 4 kinds in following 8 kinds of microorganisms at least, and its parts by weight are:
Cellulomonas cartae 15-30 part;
Basidiomycetes 10-25 part;
Genus bacillus 5-15 part;
Aspergillus 15-35 part;
Neurospora 5-35 part;
The mould 8-20 part of wood;
Actinomycetes 15-20 part;
Yeast 5-15 part;
Carrier comprises one or both in chitosan, rice bran, wood chip, the soil.
2. afforestation rubbish efficient degradation composite fungus agent according to claim 1 is characterized in that: described cellulomonas cartae is the dinitrogen cellulomonas cartae or produces the yellowish fiber Zymomonas mobilis;
Basidiomycetes is oyster cap fungus or flat mushroom;
Genus bacillus is Bacillus licheniformis or subtilis or bacillus natto or cured shape genus bacillus or bacillus megaterium;
Aspergillus is aspergillus niger;
Neurospora is that the mould or middle intercadence spore of coarse arteries and veins spore is mould;
Wood is mould to be that viride or healthy and free from worry wood are mould;
Actinomycetes are Nocardia bacteria or small single-cell bacteria;
Yeast is candida tropicalis or produces Ruan's candiyeast or yeast saccharomyces cerevisiae.
3. the preparation method of an afforestation rubbish efficient degradation composite fungus agent as claimed in claim 1, it is characterized in that: this method may further comprise the steps:
(1) behind the inoculation of medium bacterial classification, put in 35 ℃ of shaking tables 120 rev/mins, shaking culture 48-72 hour, cultivate suitably to dilute after finishing and form bacterium liquid, making every milliliter of bacterium number is 500,000,000, i.e. 500,000,000/milliliter;
(2) the bacterium number of bacterium liquid by every gram carrier 200,000,000 is adsorbed onto on the carrier, makes the efficient degradation composite fungus agent of afforestation rubbish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009100700737A CN101988040A (en) | 2009-08-06 | 2009-08-06 | Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof |
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| CN2009100700737A CN101988040A (en) | 2009-08-06 | 2009-08-06 | Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof |
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| CN101988040A true CN101988040A (en) | 2011-03-23 |
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| CN2009100700737A Pending CN101988040A (en) | 2009-08-06 | 2009-08-06 | Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391949A (en) * | 2011-11-17 | 2012-03-28 | 苏柯汉（潍坊）生物工程有限公司 | Food waste fermentation composite bacteria and preparation method thereof |
| CN102533553A (en) * | 2011-12-29 | 2012-07-04 | 湖南省微生物研究所 | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof |
| CN102559414A (en) * | 2012-01-09 | 2012-07-11 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Biological degreasing agent |
| CN102586145A (en) * | 2012-02-13 | 2012-07-18 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Garbage fermentation agent |
| CN102850098A (en) * | 2012-09-29 | 2013-01-02 | 南开大学 | Production method of efficient odorless organic fertilizer |
| CN103898032A (en) * | 2014-04-25 | 2014-07-02 | 江苏联海生物科技有限公司 | Complex microbial inoculant and method for preparing organic fertilizer by using same |
| CN104403971A (en) * | 2014-11-28 | 2015-03-11 | 蒋小春 | Straw decomposing agent and preparation method thereof |
| CN104973896A (en) * | 2015-06-18 | 2015-10-14 | 滁州市瑞景园林建设有限公司 | Nuisanceless processing method of gardening and greening garbage |
| CN105969699A (en) * | 2016-07-12 | 2016-09-28 | 佛山杰致信息科技有限公司 | Agricultural ecological probiotic composite microbial inoculant |
| CN107081328A (en) * | 2017-06-06 | 2017-08-22 | 芜湖华盈自动化设备有限公司 | One kind removes consumer waste incineration Wei gas Zhong bioxin methods |
| CN108285879A (en) * | 2017-12-12 | 2018-07-17 | 昆山合纵生态科技有限公司 | A kind of drug and preparation method thereof of fast degradation fallen leaves |
| CN109626555A (en) * | 2019-01-21 | 2019-04-16 | 四川清和科技有限公司 | A kind of method of fast degradation water body withered fallen leaf |
| CN109971677A (en) * | 2019-03-26 | 2019-07-05 | 陕西省西安植物园 | A kind of microbial germ powder and preparation method thereof |
| WO2022141141A1 (en) * | 2020-12-30 | 2022-07-07 | 义乌市绳墨新材料有限公司 | Method for degrading and treating garbage using microorganism aerobic fermentation |
-
2009
- 2009-08-06 CN CN2009100700737A patent/CN101988040A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391949A (en) * | 2011-11-17 | 2012-03-28 | 苏柯汉（潍坊）生物工程有限公司 | Food waste fermentation composite bacteria and preparation method thereof |
| CN102391949B (en) * | 2011-11-17 | 2013-07-31 | 苏柯汉（潍坊）生物工程有限公司 | Food waste fermentation composite bacteria and preparation method thereof |
| CN102533553A (en) * | 2011-12-29 | 2012-07-04 | 湖南省微生物研究所 | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof |
| CN102559414A (en) * | 2012-01-09 | 2012-07-11 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Biological degreasing agent |
| CN102586145A (en) * | 2012-02-13 | 2012-07-18 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Garbage fermentation agent |
| CN102850098A (en) * | 2012-09-29 | 2013-01-02 | 南开大学 | Production method of efficient odorless organic fertilizer |
| CN103898032A (en) * | 2014-04-25 | 2014-07-02 | 江苏联海生物科技有限公司 | Complex microbial inoculant and method for preparing organic fertilizer by using same |
| CN104403971A (en) * | 2014-11-28 | 2015-03-11 | 蒋小春 | Straw decomposing agent and preparation method thereof |
| CN104973896A (en) * | 2015-06-18 | 2015-10-14 | 滁州市瑞景园林建设有限公司 | Nuisanceless processing method of gardening and greening garbage |
| CN105969699A (en) * | 2016-07-12 | 2016-09-28 | 佛山杰致信息科技有限公司 | Agricultural ecological probiotic composite microbial inoculant |
| CN107081328A (en) * | 2017-06-06 | 2017-08-22 | 芜湖华盈自动化设备有限公司 | One kind removes consumer waste incineration Wei gas Zhong bioxin methods |
| CN108285879A (en) * | 2017-12-12 | 2018-07-17 | 昆山合纵生态科技有限公司 | A kind of drug and preparation method thereof of fast degradation fallen leaves |
| CN109626555A (en) * | 2019-01-21 | 2019-04-16 | 四川清和科技有限公司 | A kind of method of fast degradation water body withered fallen leaf |
| CN109971677A (en) * | 2019-03-26 | 2019-07-05 | 陕西省西安植物园 | A kind of microbial germ powder and preparation method thereof |
| WO2022141141A1 (en) * | 2020-12-30 | 2022-07-07 | 义乌市绳墨新材料有限公司 | Method for degrading and treating garbage using microorganism aerobic fermentation |
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Application publication date: 20110323 |