CN108285879A - A kind of drug and preparation method thereof of fast degradation fallen leaves - Google Patents
A kind of drug and preparation method thereof of fast degradation fallen leaves Download PDFInfo
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- CN108285879A CN108285879A CN201711319320.3A CN201711319320A CN108285879A CN 108285879 A CN108285879 A CN 108285879A CN 201711319320 A CN201711319320 A CN 201711319320A CN 108285879 A CN108285879 A CN 108285879A
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Abstract
The present invention relates to technical field of biological fermentation, a kind of drug and preparation method thereof of fast degradation fallen leaves, ingredient includes：Produce the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch bacillus, fixed nitrogen orphan bacterium, bacillus subtilis, cellulase, pectase, additive and culture medium；Fallen leaves prepared by present invention degradation drug to fall leaves, and degradation speed is fast, good degrading effect, saves a large amount of man power and materials in degradation process so that cost reduction of entirely degrading uses convenient for large scale investment；Cellulase and pectase are added in raw material so that leaf degradation speed is accelerated in culture medium preparation process, and various strains is promoted to survive on culture medium procreation；Being used as additive by peat, diatom and winnofil makes various strain dispersions be arranged in culture medium, contributes to strain to multiply and spread so that leaf degradation speed is accelerated.
Description
Technical field
The present invention relates to technical field of biological fermentation, and in particular to a kind of drug of fast degradation fallen leaves and its preparation side
Method.
Background technology
In recent years, environmental consciousness deepens continuously the popular feeling, and for afforestation, the understanding of shared status in city has people
Further distillation.With the development of science and technology, the raising of social progress and living standards of the people, China is by city garden
The development of woods greening and level mention the height of protecting ecology balance to recognize.With China's rapid development of economy, give birth to per capita
Running water is flat to be continuously improved, and the requirement to " livable property " is also continuously increased.Since 2010, multiple provinces and cities of China are to real estate greenery patches
Rate, which proposes, to be clearly required.Afforestation maintenance management obtains in recent years as very important link in Landscape Industry
Higher and higher attention, only Beijing's city's green areas are just up to 63,540 hectares.
Tree deciduous leaf in garden greenland does not have no the junk of purposes instead of, there is the treasured that value is widely used
Your resource.If being scientifically used, unexpected all Multi benefits can be generated.Burning disposal is carried out after being collected to fallen leaves,
Or transport to soot and filled, can all increase paying for human and material resources and financial resources, cause pollution to ecological environment and
The destruction of Natural Circulation.
Current country vigorously advocates low-carbon life and the development and utilization of new energy.With national urbanization process
The needs of accelerated development, afforestation and urban beautification, every year because fall leaves burn caused by environmental pollution and carbon dioxide
Discharge capacity present rapid growth situation.
Therefore, in view of the above problems, occurring many resolving ideas now, wherein biodegradable effect is also possible that still now
Having biodegradation technique to fall leaves deformed limb, degradation speed is slow, degradation effect is undesirable, consumes a large amount of human and material resources so that
Cost is more than advantageous effect.
Invention content
In view of the deficiencies of the prior art, the present invention provides the drugs fallen leaves with a kind of fast degradation.Meanwhile the present invention
Additionally provide kind of a preparation method for the drug of fast degradation fallen leaves.
In order to achieve the above object, the present invention is achieved by the following technical programs：
A kind of drug of fast degradation fallen leaves, ingredient is by weight：It is bacterium 1.5-2.2 parts unicellular, long to produce yellowish fiber
0.6-1.1 parts of handle wood fungus, 0.5-0.8 parts of aspergillus niger, 1.1-1.6 parts of bacillus licheniformis, 0.6-1.2 parts of fiber orphan bacterium, arch
0.9-1.5 parts of bacillus, 2.1-2.8 parts of fixed nitrogen orphan bacterium, 2.6-3.3 parts of bacillus subtilis, 2.8-3.6 parts of cellulase, pectin
72-80 parts of 2.3-2.6 parts of enzyme, 6.8-8.2 parts of additive and culture medium.
Preferably, a kind of drug of fast degradation fallen leaves, preparation method includes the following steps：
A, will produce the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch bacillus,
Fixed nitrogen orphan bacterium and bacillus subtilis are placed in 28 DEG C of culture 48h or more in PDA culture medium, and total bacteria count is 109 in every milliliter of microbial inoculum
More than a, mixed bacteria liquid is obtained, it is spare；
B, leaf is pre-processed：By leaf and dilute hydrochloric acid with mass ratio 1：2 mixing, are placed in high pressure sterilization；Wait for temperature liter
To 140-180 DEG C, then high pressure sterilization 100-120min washs leaf with deionized water, and the PH of washing to cleaning solution is
6.5-7.5, and be filtered, leaf is placed in dryer and is dried, 55-65 DEG C of drying temperature obtains sterile dry leaf,
It is spare；
C, by sterile dry leaf and the culture medium in step b according to leaf 0.6-1.2g is added in every milliliter of culture medium,
Then cellulase, pectase and additive are added in culture medium, control culture medium temperature is 42-50 DEG C, cultivates 18-
For 24 hours, leaf degradation culture medium is obtained；
D, mixed bacteria liquid is added in leaf degradation culture medium, wherein the Mixed Microbes of 1.3-1.8ml are added in every gram of leaf
Liquid is placed in the desinfection chamber that temperature is 46-52 DEG C, and 36-48h is to get finished product for fermentation.
Preferably, the additive selects peat, diatom and winnofil peace according to mass ratio 1： 1：1 mixes.
Preferably, the culture medium includes 5g/L peptones, 1g/L yeast extract powders, 5g/L sodium chloride, 5g/L corn stalks
Stalk, 2.0g/L CaCO3, 10mL/L soil extractions, remaining ingredient be sterile water.
Preferably, pressure when sterilizing in the step b in high-pressure sterilizing pot is 0.5-0.7MPa.
Preferably, the pH value of dilute hydrochloric acid is 5.5-6.2 in the step b.
Advantageous effect：
The present invention is to produce the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch
Bacillus, fixed nitrogen orphan bacterium, bacillus subtilis, cellulase, pectase, additive and culture medium are as preparation fallen leaves degradation medicine
The raw material of object, prepared fallen leaves degradation drug to fall leaves, and degradation speed is fast, good degrading effect, is saved in degradation process big
Measure man power and material so that cost reduction of entirely degrading uses convenient for large scale investment.Cellulase is added in raw material
And pectase so that leaf degradation speed is accelerated in culture medium preparation process, promotes various strains to survive on culture medium numerous
Spread out.Being used as additive by peat, diatom and winnofil makes various strain dispersions be arranged in culture medium, contributes to bacterium
Kind procreation and diffusion so that leaf degradation speed is accelerated.In addition, the present invention is by the culture for adding mixed bacteria liquid in step b
Base, which is placed in desinfection chamber, to ferment so that other bacteriums is not present in drug so that drug strain is pure, while bacterium in drug
Growth and multiplying speed are accelerated.
Specific implementation mode
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, implement below in conjunction with the present invention
Example, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention
A part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
There is the every other embodiment obtained under the premise of making creative work, shall fall within the protection scope of the present invention.
Embodiment 1：
A kind of drug of fast degradation fallen leaves, ingredient is by weight：Produce unicellular 1.5 parts of the bacterium of yellowish fiber, long handle wood
0.9 part of bacterium, 0.5 part of aspergillus niger, 1.1 parts of bacillus licheniformis, 0.6 part of fiber orphan bacterium, 1.3 parts of arch bacillus, fixed nitrogen orphan bacterium
80 parts of 2.1 parts, 2.6 parts of bacillus subtilis, 2.8 parts of cellulase, 2.5 parts of pectase, 6.8 parts of additive and culture medium.
A kind of drug of fast degradation fallen leaves, preparation method includes the following steps：
A, will produce the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch bacillus,
Fixed nitrogen orphan bacterium and bacillus subtilis are placed in 28 DEG C of culture 48h or more in PDA culture medium, and total bacteria count is 109 in every milliliter of microbial inoculum
More than a, it is spare to obtain mixed bacteria liquid；
B, leaf is pre-processed：By leaf and dilute hydrochloric acid with mass ratio 1：2 mixing, are placed in high pressure sterilization；Wait for temperature liter
To 140 DEG C, then high pressure sterilization 108min washs leaf with deionized water, and it is 6.5 to wash to the PH of cleaning solution, and is carried out
Filtering, leaf is placed in dryer and is dried, 62 DEG C of drying temperature obtains sterile dry leaf, spare；
C, by sterile dry leaf and the culture medium in step b according to leaf 0.6g is added in every milliliter of culture medium, then
Cellulase, pectase and additive are added in culture medium, control culture medium temperature is 42 DEG C, cultivates 18h, is set
Leaf degradation culture medium；
D, mixed bacteria liquid is added in leaf degradation culture medium, wherein the mixed bacteria liquid of 1.8ml is added in every gram of leaf,
It is placed in the desinfection chamber that temperature is 46 DEG C, 44h is to get finished product for fermentation.
Additive selects peat, diatom and winnofil peace according to mass ratio 1：1：1 mixes.
Culture medium includes 5g/L peptones, 1g/L yeast extract powders, 5g/L sodium chloride, 5g/L maize straws, 2.0g/L
CaCO3, 10mL/L soil extractions, remaining ingredient be sterile water.
Pressure when sterilizing in step b in high-pressure sterilizing pot is 0.5MPa.
The pH value of dilute hydrochloric acid is 5.5 in step b.
Embodiment 2：
A kind of drug of fast degradation fallen leaves, ingredient is by weight：Produce unicellular 1.7 parts of the bacterium of yellowish fiber, long handle wood
0.8 part of bacterium, 0.6 part of aspergillus niger, 1.3 parts of bacillus licheniformis, 0.8 part of fiber orphan bacterium, 0.9 part of arch bacillus, fixed nitrogen orphan bacterium
72 parts of 2.3 parts, 3.1 parts of bacillus subtilis, 3.1 parts of cellulase, 2.3 parts of pectase, 7.3 parts of additive and culture medium.
A kind of drug of fast degradation fallen leaves, preparation method includes the following steps：
A, will produce the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch bacillus,
Fixed nitrogen orphan bacterium and bacillus subtilis are placed in 28 DEG C of culture 48h or more in PDA culture medium, and total bacteria count is 109 in every milliliter of microbial inoculum
More than a, it is spare to obtain mixed bacteria liquid；
B, leaf is pre-processed：By leaf and dilute hydrochloric acid with mass ratio 1：2 mixing, are placed in high pressure sterilization；Wait for temperature liter
To 155 DEG C, then high pressure sterilization 100min washs leaf with deionized water, and it is 6.8 to wash to the PH of cleaning solution, and is carried out
Filtering, leaf is placed in dryer and is dried, 55 DEG C of drying temperature obtains sterile dry leaf, spare；
C, by sterile dry leaf and the culture medium in step b according to leaf 0.8g is added in every milliliter of culture medium, then
Cellulase, pectase and additive are added in culture medium, control culture medium temperature is 48 DEG C, cultivates 20h, is set
Leaf degradation culture medium；
D, mixed bacteria liquid is added in leaf degradation culture medium, wherein the mixed bacteria liquid of 1.3ml is added in every gram of leaf,
It is placed in the desinfection chamber that temperature is 48 DEG C, 40h is to get finished product for fermentation.
Additive selects peat, diatom and winnofil peace according to mass ratio 1：1：1 mixes.
Culture medium includes 5g/L peptones, 1g/L yeast extract powders, 5g/L sodium chloride, 5g/L maize straws, 2.0g/L
CaCO3, 10mL/L soil extractions, remaining ingredient be sterile water.
Pressure when sterilizing in step b in high-pressure sterilizing pot is 0.58MPa.
The pH value of dilute hydrochloric acid is 5.7 in step b.
Embodiment 3：
A kind of drug of fast degradation fallen leaves, ingredient is by weight：Produce unicellular 1.9 parts of the bacterium of yellowish fiber, long handle wood
1.1 parts of bacterium, 0.7 part of aspergillus niger, 1.6 parts of bacillus licheniformis, 1.0 parts of fiber orphan bacterium, 1.5 parts of arch bacillus, fixed nitrogen orphan bacterium
77 parts of 2.5 parts, 2.8 parts of bacillus subtilis, 3.4 parts of cellulase, 2.4 parts of pectase, 7.7 parts of additive and culture medium.
A kind of drug of fast degradation fallen leaves, preparation method includes the following steps：
A, will produce the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch bacillus,
Fixed nitrogen orphan bacterium and bacillus subtilis are placed in 28 DEG C of culture 48h or more in PDA culture medium, and total bacteria count is 109 in every milliliter of microbial inoculum
More than a, it is spare to obtain mixed bacteria liquid；
B, leaf is pre-processed：By leaf and dilute hydrochloric acid with mass ratio 1：2 mixing, are placed in high pressure sterilization；Wait for temperature liter
To 170 DEG C, then high pressure sterilization 114min washs leaf with deionized water, and it is 7.2 to wash to the PH of cleaning solution, and is carried out
Filtering, leaf is placed in dryer and is dried, 58 DEG C of drying temperature obtains sterile dry leaf, spare；
C, by sterile dry leaf and the culture medium in step b according to leaf 1.0g is added in every milliliter of culture medium, then
Cellulase, pectase and additive are added in culture medium, control culture medium temperature is 45 DEG C, cultivates 22h, is set
Leaf degradation culture medium；
D, mixed bacteria liquid is added in leaf degradation culture medium, wherein the mixed bacteria liquid of 1.6ml is added in every gram of leaf,
It is placed in the desinfection chamber that temperature is 50 DEG C, 48h is to get finished product for fermentation.
Additive selects peat, diatom and winnofil peace according to mass ratio 1：1：1 mixes.
Culture medium includes 5g/L peptones, 1g/L yeast extract powders, 5g/L sodium chloride, 5g/L maize straws, 2.0g/L
CaCO3, 10mL/L soil extractions, remaining ingredient be sterile water.
Pressure when sterilizing in step b in high-pressure sterilizing pot is 0.54MPa.
The pH value of dilute hydrochloric acid is 5.9 in step b.
Embodiment 4：
A kind of drug of fast degradation fallen leaves, ingredient is by weight：Produce unicellular 2.2 parts of the bacterium of yellowish fiber, long handle wood
0.6 part of bacterium, 0.8 part of aspergillus niger, 1.4 parts of bacillus licheniformis, 1.2 parts of fiber orphan bacterium, 1.1 parts of arch bacillus, fixed nitrogen orphan bacterium
73 parts of 2.8 parts, 3.3 parts of bacillus subtilis, 3.6 parts of cellulase, 2.6 parts of pectase, 8.2 parts of additive and culture medium.
A kind of drug of fast degradation fallen leaves, preparation method includes the following steps：
A, will produce the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch bacillus,
Fixed nitrogen orphan bacterium and bacillus subtilis are placed in 28 DEG C of culture 48h or more in PDA culture medium, and total bacteria count is 109 in every milliliter of microbial inoculum
More than a, it is spare to obtain mixed bacteria liquid；
B, leaf is pre-processed：By leaf and dilute hydrochloric acid with mass ratio 1：2 mixing, are placed in high pressure sterilization；Wait for temperature liter
To 180 DEG C, then high pressure sterilization 120min washs leaf with deionized water, and it is 7.5 to wash to the PH of cleaning solution, and is carried out
Filtering, leaf is placed in dryer and is dried, 65 DEG C of drying temperature obtains sterile dry leaf, spare；
C, by sterile dry leaf and the culture medium in step b according to leaf 1.2g is added in every milliliter of culture medium, then
Cellulase, pectase and additive are added in culture medium, control culture medium temperature is 50 DEG C, and culture for 24 hours, is set
Leaf degradation culture medium；
D, mixed bacteria liquid is added in leaf degradation culture medium, wherein the mixed bacteria liquid of 1.5ml is added in every gram of leaf,
It is placed in the desinfection chamber that temperature is 52 DEG C, 36h is to get finished product for fermentation.
Additive selects peat, diatom and winnofil peace according to mass ratio 1：1：1 mixes.
Culture medium includes 5g/L peptones, 1g/L yeast extract powders, 5g/L sodium chloride, 5g/L maize straws, 2.0g/L
CaCO3, 10mL/L soil extractions, remaining ingredient be sterile water.
Pressure when sterilizing in step b in high-pressure sterilizing pot is 0.7MPa.
The pH value of dilute hydrochloric acid is 6.2 in step b.
It should be noted that herein, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, without necessarily requiring or implying between these entities or operation
There are any actual relationship or orders.Moreover, the terms "include", "comprise" or its any other variant are intended to
Cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements includes not only that
A little elements, but also include other elements that are not explicitly listed, or further include for this process, method, article or
The intrinsic element of equipment.In the absence of more restrictions, the element limited by sentence "including a ...", not
There is also other identical elements in the process, method, article or apparatus that includes the element for exclusion.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, it will be understood by those of ordinary skill in the art that：It still can be to aforementioned each implementation
Technical solution recorded in example is modified or equivalent replacement of some of the technical features；And these modification or
It replaces, the spirit and scope for various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution.
Claims (6)
1. a kind of drug of fast degradation fallen leaves, which is characterized in that its ingredient is by weight：Produce the unicellular bacterium 1.5- of yellowish fiber
2.2 parts, 0.6-1.1 parts of long handle wood fungus, 0.5-0.8 parts of aspergillus niger, 1.1-1.6 parts of bacillus licheniformis, fiber orphan bacterium 0.6-1.2
Part, 0.9-1.5 parts of arch bacillus, 2.1-2.8 parts of fixed nitrogen orphan bacterium, 2.6-3.3 parts of bacillus subtilis, cellulase 2.8-3.6
Part, 2.3-2.6 parts of pectase, 6.8-8.2 parts of additive and 72-80 parts of culture medium.
2. a kind of drug of fast degradation fallen leaves according to claim 1, which is characterized in that preparation method includes following
Step：
A, the unicellular bacterium of yellowish fiber, long handle wood fungus, aspergillus niger, bacillus licheniformis, fiber orphan bacterium, arch bacillus, fixed nitrogen will be produced
Lonely bacterium and bacillus subtilis are placed in PDA culture medium 28 DEG C of culture 48h or more, in every milliliter of microbial inoculum total bacteria count be 109 with
On, mixed bacteria liquid is obtained, it is spare；
B, leaf is pre-processed：By leaf and dilute hydrochloric acid with mass ratio 1：2 mixing, are placed in high pressure sterilization；Wait for that temperature rises to
140-180 DEG C, then high pressure sterilization 100-120min washs leaf with deionized water, and it is 6.5- to wash to the PH of cleaning solution
7.5, and be filtered, leaf is placed in dryer and is dried, 55-65 DEG C of drying temperature obtains sterile dry leaf, spare；
C, by sterile dry leaf and the culture medium in step b according to leaf 0.6-1.2g is added in every milliliter of culture medium, then
Cellulase, pectase and additive are added in culture medium, control culture medium temperature is 42-50 DEG C, cultivates 18-24h, obtains
To leaf degradation culture medium；
D, mixed bacteria liquid is added in leaf degradation culture medium, wherein the mixed bacteria liquid of 1.3-1.8ml is added in every gram of leaf, is set
In the desinfection chamber that temperature is 46-52 DEG C, 36-48h is to get finished product for fermentation.
3. a kind of drug of fast degradation fallen leaves according to claim 1, it is characterised in that：The additive selects mud
Charcoal, diatom and winnofil peace are according to mass ratio 1：1：1 mixes.
4. a kind of drug of fast degradation fallen leaves according to claim 1, it is characterised in that：The culture medium includes 5g/L
Peptone, 1g/L yeast extract powders, 5g/L sodium chloride, 5g/L maize straws, 2.0g/L CaCO3, 10mL/L soil extractions,
Remaining ingredient is sterile water.
5. a kind of preparation method of the drug of fast degradation fallen leaves according to claim 2, it is characterised in that：The step
Pressure when sterilizing in b in high-pressure sterilizing pot is 0.5-0.7MPa.
6. a kind of preparation method of the drug of fast degradation fallen leaves according to claim 2, it is characterised in that：The step
The pH value of dilute hydrochloric acid is 5.5-6.2 in b.
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| CN201711319320.3A CN108285879A (en) | 2017-12-12 | 2017-12-12 | A kind of drug and preparation method thereof of fast degradation fallen leaves |
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| CN201711319320.3A CN108285879A (en) | 2017-12-12 | 2017-12-12 | A kind of drug and preparation method thereof of fast degradation fallen leaves |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109626555A (en) * | 2019-01-21 | 2019-04-16 | 四川清和科技有限公司 | A kind of method of fast degradation water body withered fallen leaf |
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| WO2002014540A1 (en) * | 2000-08-11 | 2002-02-21 | Cornell Research Foundation, Inc. | Production and use of inducible enzymes from trichoderma and bacteria for control of plant pests and for industrial processes |
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- 2017-12-12 CN CN201711319320.3A patent/CN108285879A/en active Pending
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|---|---|---|---|---|
| WO2002014540A1 (en) * | 2000-08-11 | 2002-02-21 | Cornell Research Foundation, Inc. | Production and use of inducible enzymes from trichoderma and bacteria for control of plant pests and for industrial processes |
| CN101988040A (en) * | 2009-08-06 | 2011-03-23 | 南开大学 | Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof |
| CN104004694A (en) * | 2014-06-19 | 2014-08-27 | 内蒙古农业大学 | Low temperature efficient corn stalk degradation composite microbial system and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109626555A (en) * | 2019-01-21 | 2019-04-16 | 四川清和科技有限公司 | A kind of method of fast degradation water body withered fallen leaf |
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