CN103540538A - Killer yeast Xiaoqu koji and preparation method thereof - Google Patents

Killer yeast Xiaoqu koji and preparation method thereof Download PDF

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CN103540538A
CN103540538A CN201310307805.6A CN201310307805A CN103540538A CN 103540538 A CN103540538 A CN 103540538A CN 201310307805 A CN201310307805 A CN 201310307805A CN 103540538 A CN103540538 A CN 103540538A
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killer yeast
culture
yeast
koji
killer
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CN103540538B (en
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张凤英
陈卫平
张涛
罗秋水
涂瑾
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Jiangxi Agricultural University
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Abstract

The invention discloses killer yeast Xiaoqu koji and a preparation method thereof. The killer yeast Xiaoqu koji is obtained by uniformly mixing 87-94 parts by weight of rhizopus Q303 rice flour or wheat bran culture and 6-13 parts by weight of mixed grain culture of killer yeast Sa2 and packaging. According to the preparation method disclosed by the invention, the killer yeast Sa2 which can effectively inhibit the pollution of hybrid bacteria and rhizopus Q303 which can well utilize rice starch are selected as functional bacteria of the killer yeast Xiaoqu koji. The hybrid bacteria in an environment can be effectively prevented from polluting koji material and liquor-brewing raw materials, a culture or fermentation system is purified, and the smooth progress of koji-making and liquor-brewing process can be further ensured. The alcohol degree of mash formed by using the killer yeast Sa2 Xiaoqu koji to ferment polished long-grained non-glutinous rice can achieve 15.2 degrees, while the highest alcohol degree by using the ordinary alcohol yeast Xiaoqu koji is only about 13 degrees. The killer yeast Sa2 Xiaoqu koji has an obvious effect of improving liquor yield and can prevent reduction of the quality of liquor. In both the production of Xiaoqu and the liquor-brewing process, the energy can be saved, and the using efficiency of equipment can be improved, and koji burning and acid liquor can be further prevented in summer.

Description

Little sort of quyi of killer yeast and preparation method thereof
Technical field
The present invention relates to white wine and brewing yellow rice wine field, particularly relate to a kind of little sort of quyi of killer yeast for gently aromatic white spirit and brewing yellow rice wine, little bent production and preparation method thereof.
Background technology
Chinese wine has the concentrated and accumulation of culture in 5,000 years, the medium of linking up as a kind of interpersonal relation, and it still plays irreplaceable effect in actual life.Develop into now, China white wine has become output maximum in six large liquors in the world, liquor that kind is maximum.Its medium and small bent method white wine is one of main methodology of the light aromatic white spirit production of China, and its output accounts for the more than 70% of ultimate production; Yellow rice wine, as the Chinese quintessence of Chinese culture, also has the huge consumer group, is the main wine kind of Chinese's health care, is the main base wine of medicinal liquor, and another kind-cooking wine of yellow rice wine has been come into restaurant, public house, huge numbers of families especially, becomes the Chinese common people's cooking and must use wine kind.And the production of yellow rice wine and little bent method white wine is all to use little koji fermentation to brewage to form.In provinces such as China Guangxi, Guangdong, Fujian, Hainan and the some areas in Hunan, Hubei, Guizhou, Zhejiang, Jiangsu, especially distilled liquor consumption in rural area accounts for greater advantage in all kinds of white wine.
Killer yeast refers to that those, can be to the yeast strain of a kind of toxin protein of cell exocrine in its growth and breeding process, and this toxin protein can kill the sensitive strain of same genus, in less situation, also can kill the sensitive strain not belonging to together.The toxin protein of killer yeast secretion is called killer toxin, and killer yeast has immunizing power to the killer toxin of self secretion.The sanguinariness characteristic of killer yeast can prevent the pollution of wild miscellaneous bacteria, prevents the poor product quality causing due to living contaminants or the problem yielding poorly, and reaches purification fermentation system, guarantees the object that fermentation is normally carried out.
Abroad, people, at grape wine, have used sanguinariness bacterial strain to purify fermentation system in beer production.Domestic, also carried out in recent years the research work of killer yeast aspect.But the research of relevant killer yeast, only has the killer yeast for beer and wine fermentation.The killer yeast that the present invention selects is compared with general alcohol active dried yeast, can improve fermenting power, improves the yield of liquor, stablizes vinosity; Both wild yeast can have been suppressed to belong to together, the pollution of the miscellaneous bacteria that can suppress not belong to together again.The killer yeast of having reported only has killing effect to responsive yeast and wild yeast.
Up to the present,, there is no the report of producing for distiller's yeast about killer yeast at home and abroad.
Little song in the market, by ethyl alcoh(ol) yeast or active dry yeast and pure head mold Qu Peicheng, does not have restraining effect to the miscellaneous bacteria in song material, and the sterilization of bent material is had relatively high expectations, and sterilization energy consumption is high, otherwise easily occurs to burn bent and sour wine phenomenon, especially in summer.
Summary of the invention
First object of the present invention is to provide a kind of killer yeast the little sort of quyi, the little sort of quyi of this killer yeast can effectively prevent the bent material of the living contaminants in environment, thereby purified fermentation system, guaranteed carrying out smoothly of koji, wine brewing process, thereby and on the basis that does not reduce vinosity, can effectively suppress varied bacteria growing and not only can prevent sour wine, improve the yield of liquor, also can reduce sterilization energy consumption.
Second object of the present invention is to provide the preparation method of the little sort of quyi of a kind of killer yeast.
First object of the present invention is achieved in that
A little sort of quyi, prepare bacterial classification and be yeast saccharomyces cerevisiae ( saccharomyces cerevisiae)be preserved in (address: 100001 No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institutes of microbiology institute of the Chinese Academy of Sciences), China Microbial Culture Preservation Commission's common micro-organisms center, preservation date is on 04 16th, 2012, preserving number: CGMCC No.6015.
Killer yeast Sa2 ( sacchaormyces cerevisiaesa2 )culture condition be: optimal medium for improvement bean sprout juice; PH nature; Culture temperature 28-32 ℃; Incubation time 16-24hr.
A little sort of quyi, feature is: formula is counted with parts by weight: head mold Q303 ground rice or wheat bran culture: 87-94 part, the blended grain culture of killer yeast Sa2: 6-13 part.
Second object of the present invention is achieved in that
A preparation method for the little sort of quyi of killer yeast, feature is:
The preparation of A, head mold Q303 ground rice or wheat bran culture: first pack the 80-160 gram of wheat bran that water content is 40-60% by weight percentage or water content is 30-40% by weight percentage ground rice in the triangular flask of 500-1000ml into, after sterilization is cooling, the spore suspension of the slant tube kind of access 6-10ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 40-48 hour, obtain head mold Q303 ground rice or wheat bran culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 100-200g, put into pot and boil 20-30 minute, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add again glucose 80-120g, dipotassium hydrogen phosphate 1g, sal epsom 0.3-0.5 g, Repone K 0.2-0.4g, sodium-chlor 1-2g, peptone 10-15g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack maslin that 70-130 gram is 35-50% containing the peptone water yield by weight percentage in the triangular flask of 500--1000ml into (wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%), after sterilizing is cooling, by in the spore suspension access triangular flask of the slant tube kind of 4-7ml head mold Q303, cultivate 16-24 hour for 28-32 ℃; Access again the improvement bean sprout juice nutrient solution test tube kind 8-20ml of killer yeast, cultivate 22-28 hour for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively ground rice or the wheat bran culture of the head mold Q303 of meter: 87-94 part, the blended grain culture of killer yeast: 6-13 part, the blended grain culture of the ground rice of head mold Q303 or wheat bran culture and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.
The killer yeast sort of quyi of the present invention can effectively prevent the bent material of the living contaminants in environment or liquor-making raw material, thereby purified fermentation system, guarantee carrying out smoothly of koji, wine brewing process, and on the basis that does not reduce vinosity, can effectively suppress varied bacteria growing, prevent sour wine, improved the yield of liquor.
Therefore, tool of the present invention has the following advantages: 1, killer yeast Sa2 can not only kill and belong to responsive yeast together, also can kill the sensitive strain not belonging to together, thereby in yeast making process, main miscellaneous bacteria in song material is had to stronger restraining effect, can effectively prevent the bent material of living contaminants in environment; In when wine brewing, also can purify fermentation system, prevent from rising acid too high, avoid mash to produce different assorted taste, reduction potato spirit content; 2, killer yeast Sa2 does not affect growth and breeding and the saccharogenic power of amylomyces head mold in koji or wine brewing process, has guaranteed carrying out smoothly of koji or wine brewing process; 3, the fermentation condition of killer yeast Sa2 the most suitable growth is close with conventional distillery yeast; 4, the distillery yeast that killer yeast Sa2 is conventional with current koji is compared and is shown better high temperature resistant, resistance to high alcohol and Nai Gao sugar characteristic, can save energy in the production of Xiaoqu wine and yellow rice wine, improve the utilising efficiency of equipment; 5, with 15.2 ° of the mash wine Du Keda of killer yeast Sa2 fermentation long-grained nonglutinous rice, with ethyl alcoh(ol) yeast is the highest, only have 13 ° of left and right, killer yeast Sa2 has obvious effect to improving the yield of liquor, also can anti-burnings song and sour wine in summer, raising vinosity and the yield of liquor; 6, killer yeast Sa2 bacterial classification genetic stability is good, easily cultivation, easily preservation.
If implement with killer yeast Sa2 the advantage that raw grog mixed culture technology has traditional koji and modern starter making method concurrently: implementing raw grog mixed culture with killer yeast Sa2 can be on the basis of effective reduction energy consumption, and do not increase and on the basis of production cost, produce the little song that diastatic fermentation power and well done material song are equally high.In addition, killer yeast Sa2 mixes and produces little song for raw grog, to improving raw grog, mixes the quality of producing little song, reduces production energy consumption and improves little bent production technique and can play a key effect.
Figure 457725DEST_PATH_IMAGE001
Be proven, with 100 jin of rice of killer yeast Sa2 sort of quyi fermentation, can obtain 100 jin of 40-43 degree white wine, and with general distillery yeast wine degree the highest only have 100 jin of 36-40 degree white wine.And by the killer yeast Sa2 sort of quyi of the present invention, produce the vinosity of wine equally good with the vinosity of commonly using at present distillery yeast production, and stable yield.The present invention can also can produce the good distilled liquor of quality, liquor bran koji for the production of liquor for the production of brewing wine, and yellow rice wine, can improve raw material availability and equipment service efficiency, can reduce sterilization energy consumption, has good promotion prospect.
Adopt the blended grain culture of killer yeast than directly using commercially available active dry yeast, to mouthfeel, improve also helpful.
Embodiment
Below in conjunction with embodiment, the present invention is further detailed.
Embodiment 1:
A preparation method for the little sort of quyi of killer yeast, concrete steps are as follows:
The preparation of A, head mold Q303 ground rice culture: first pack 90 grams of ground rice that water content is 38% by weight percentage in the triangular flask of 500ml into, after sterilization is cooling, the slant tube kind spore suspension of access 7ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 40 hours, obtain head mold Q303 ground rice culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 100g, boil 20 minutes, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add glucose 80 g, dipotassium hydrogen phosphate 1g, sal epsom 0.3 g, Repone K 0.2g, sodium-chlor 1g, peptone 11g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack maslin that 90 grams is 40% containing the peptone water yield by weight percentage in the triangular flask of 500ml into (wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%), after sterilizing is cooling, by the spore suspension access triangular flask of the slant tube kind of 5ml head mold Q303 28-32 ℃ cultivate 16 hours; Access again the improvement bean sprout juice nutrient solution test tube kind 10ml of killer yeast, cultivate 23 hours for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively the ground rice culture of the head mold Q303 of meter: 88 parts, the blended grain culture of killer yeast: 12 parts, the blended grain culture of the ground rice culture of head mold Q303 and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.
Embodiment 2:
A preparation method for the little sort of quyi of killer yeast, concrete steps are as follows:
The preparation of A, head mold Q303 ground rice culture: first pack 110 grams of ground rice that water content is 35% by weight percentage in the triangular flask of 750ml into, after sterilization is cooling, the slant tube kind spore suspension of access 8ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 44 hours, obtain head mold Q303 ground rice culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 150g, boil 25 minutes, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add glucose 100 g, dipotassium hydrogen phosphate 1g, sal epsom 0.4 g, Repone K 0.3g, sodium-chlor 1.5g, peptone 13g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack maslin that 110 grams is 45% containing the peptone water yield by weight percentage in the triangular flask of 750ml into (wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%), after sterilizing is cooling, by the spore suspension access triangular flask of the slant tube kind of 6ml head mold Q303 28-32 ℃ cultivate 18 hours; Access again the improvement bean sprout juice nutrient solution test tube kind 15ml of killer yeast, cultivate 25 hours for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively the ground rice culture of the head mold Q303 of meter: 90 parts, the blended grain culture of killer yeast: 10 parts, the blended grain culture of the ground rice culture of head mold Q303 and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.
Embodiment 3:
A preparation method for the little sort of quyi of killer yeast, concrete steps are as follows:
The preparation of A, head mold Q303 ground rice culture: first pack 130 grams of ground rice that water content is 32% by weight percentage in the triangular flask of 1000ml into, after sterilization is cooling, the slant tube kind spore suspension of access 9ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 48 hours, obtain head mold Q303 ground rice culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 200g, boil 30 minutes, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add glucose 120 g, dipotassium hydrogen phosphate 1g, sal epsom 0.5 g, Repone K 0.4g, sodium-chlor 2g, peptone 15g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack maslin that 130 grams is 50% containing the peptone water yield by weight percentage in the triangular flask of 1000ml into (wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%), after sterilizing is cooling, by the spore suspension access triangular flask of the slant tube kind of 7ml head mold Q303 28-32 ℃ cultivate 20 hours; Access again the improvement bean sprout juice nutrient solution test tube kind 20ml of killer yeast, cultivate 28 hours for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively the ground rice culture of the head mold Q303 of meter: 93 parts, the blended grain culture of killer yeast: 7 parts, the blended grain culture of the ground rice culture of head mold Q303 and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.
Embodiment 4:
A preparation method for the little sort of quyi of killer yeast, concrete steps are as follows:
The preparation of A, head mold Q303 wheat bran culture: first packing 80 grams in the triangular flask of 500ml is the wheat bran of water content 55% by weight percentage, after sterilization is cooling, the slant tube kind spore suspension of access 8ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 44 hours, obtain head mold Q303 wheat bran culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 100g, boil 20 minutes, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add glucose 80 g, dipotassium hydrogen phosphate 1g, sal epsom 0.3 g, Repone K 0.2g, sodium-chlor 1g, peptone 11g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack maslin that 90 grams is 40% containing the peptone water yield by weight percentage in the triangular flask of 500ml into (wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%), after sterilizing is cooling, by the spore suspension access triangular flask of the slant tube kind of 5ml head mold Q303 28-32 ℃ cultivate 16 hours; Access again the improvement bean sprout juice nutrient solution test tube kind 10ml of killer yeast, cultivate 23 hours for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively the head mold Q303 wheat bran culture of meter: 90 parts, the blended grain culture of killer yeast: 10 parts, the blended grain culture of the wheat bran culture of head mold Q303 and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.
Embodiment 5:
A preparation method for the little sort of quyi of killer yeast, concrete steps are as follows:
The preparation of A, head mold Q303 wheat bran culture: first packing 100 grams in the triangular flask of 750ml is the wheat bran of water content 50% by weight percentage, after sterilization is cooling, the slant tube kind spore suspension of access 9ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 46 hours, obtain head mold Q303 wheat bran culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 150g, boil 25 minutes, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add glucose 100 g, dipotassium hydrogen phosphate 1g, sal epsom 0.4 g, Repone K 0.3g, sodium-chlor 1.5g, peptone 13g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack maslin that 110 grams is 45% containing the peptone water yield by weight percentage in the triangular flask of 1000ml into (wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%), after sterilizing is cooling, by the spore suspension access triangular flask of the slant tube kind of 6ml head mold Q303 28-32 ℃ cultivate 18 hours; Access again the improvement bean sprout juice nutrient solution test tube kind 15ml of killer yeast, cultivate 25 hours for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively the head mold Q303 wheat bran culture of meter: 92 parts, the blended grain culture of killer yeast: 8 parts, the blended grain culture of the wheat bran culture of head mold Q303 and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.
Embodiment 6:
A preparation method for the little sort of quyi of killer yeast, concrete steps are as follows:
The preparation of A, head mold Q303 wheat bran culture: first packing 120 grams in the triangular flask of 1000ml is the wheat bran of water content 45% by weight percentage, after sterilization is cooling, the slant tube kind spore suspension of access 10ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 48 hours, obtain head mold Q303 wheat bran culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 200g, boil 30 minutes, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add glucose 120 g, dipotassium hydrogen phosphate 1g, sal epsom 0.5 g, Repone K 0.4g, sodium-chlor 2g, peptone 15g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack maslin that 130 grams is 50% containing the peptone water yield by weight percentage in the triangular flask of 1000ml into (wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%), after sterilizing is cooling, by the spore suspension access triangular flask of the slant tube kind of 7ml head mold Q303 28-32 ℃ cultivate 20 hours; Access again the improvement bean sprout juice nutrient solution test tube kind 20ml of killer yeast, cultivate 28 hours for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively the head mold Q303 wheat bran culture of meter: 94 parts, the blended grain culture of killer yeast: 6 parts, the blended grain culture of the wheat bran culture of head mold Q303 and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.

Claims (4)

1. the little sort of quyi of killer yeast, is characterized in that: for killer yeast Sa2 ( sacchaormyces cerevisiaesa2 ), being preserved in China Microbial Culture Preservation Commission's common micro-organisms center, preservation date is on 04 16th, 2012, preserving number: CGMCC No.6015.
2. the little sort of quyi of killer yeast according to claim 1, is characterized in that: killer yeast Sa2 ( sacchaormyces cerevisiaesa2) culture condition is: optimal medium is improvement bean sprout juice; PH nature; Culture temperature 28-32 ℃; Incubation time 16-24hr.
3. the little sort of quyi of killer yeast, is characterized in that: formula is counted with parts by weight: head mold Q303 ground rice or wheat bran culture: 87-94 part, the blended grain culture of killer yeast Sa2: 6-13 part.
4. a preparation method for the little sort of quyi of killer yeast, is characterized in that:
The preparation of A, head mold Q303 ground rice or wheat bran culture:
First in the triangular flask of 500-1000ml, pack the 80-160 gram of wheat bran that water content is 40-60% by weight percentage or water content is 30-40% by weight percentage ground rice into, after sterilization is cooling, the spore suspension of the slant tube kind of access 6-10ml head mold Q303, after shaking up, be placed in thermostat container 28-32 ℃ and cultivate 40-48 hour, obtain head mold Q303 ground rice or wheat bran culture after digging out oven dry;
The preparation of the blended grain culture of B, killer yeast Sa2:
A, preparation improvement bean sprout juice nutrient solution: get Semen Phaseoli radiati Germinatus 100-200g, put into pot and boil 20-30 minute, cooling rear by filtered through gauze, in the filtrate obtaining, add distilled water to 1000mL, add again glucose 80-120g, dipotassium hydrogen phosphate 1g, sal epsom 0.3-0.5 g, Repone K 0.2-0.4g, sodium-chlor 1-2g, peptone 10-15g, pH nature, obtains improveing bean sprout juice nutrient solution after mixed dissolution;
B, pack the maslin that 70-130 gram is 35-50% containing the peptone water yield by weight percentage in the triangular flask of 500--1000ml into, wherein: millet powder 6%, Semen Maydis powder 8%, wheat bran 86%, after sterilizing is cooling, by in the spore suspension access triangular flask of the slant tube kind of 4-7ml head mold Q303, cultivate 16-24 hour for 28-32 ℃; Access again the improvement bean sprout juice nutrient solution test tube kind 8-20ml of killer yeast, cultivate 22-28 hour for 28-30 ℃, dig out and dry the blended grain culture that obtains killer yeast Sa2;
C, take by weight respectively ground rice or the wheat bran culture of the head mold Q303 of meter: 87-94 part, the blended grain culture of killer yeast: 6-13 part, the blended grain culture of the ground rice of head mold Q303 or wheat bran culture and killer yeast is mixed, stir, after packing, obtain the little sort of quyi of killer yeast.
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